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1.
Acta Pharmacol Sin ; 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31949292

RESUMO

Metrnl is a newly identified secreted protein highly expressed in the intestinal epithelium. This study aimed to explore the role and mechanism of intestinal epithelial Metrnl in ulcerative colitis. Metrnl-/- (intestinal epithelial cell-specific Metrnl knockout) mice did not display any phenotypes of colitis under basal conditions. However, under administration of 3% dextran sodium sulfate (DSS) drinking water, colitis was more severe in Metrnl-/- mice than in WT mice, as indicated by comparisons of body weight loss, the presence of occult or gross blood per rectum, stool consistency, shrinkage in the colon, intestinal damage, and serum levels of inflammatory factors. DSS-induced colitis activated autophagy in the colon. This activation was partially inhibited by intestinal epithelial Metrnl deficiency, as indicated by a decrease in Beclin-1 and LC3-II/I and an increase in p62 in DSS-treated Metrnl-/- mice compared with WT mice. These phenomena were further confirmed by observation of autophagosomes and immunofluorescence staining for LC3 in epithelial cells. The autophagy-related AMPK-mTOR-p70S6K pathway was also activated in DSS-induced colitis, and this pathway was partially blocked by intestinal epithelial Metrnl deficiency, as indicated by a decrease in AMPK phosphorylation and an increase in mTOR and p70S6K phosphorylation in DSS-treated Metrnl-/- mice compared with WT mice. Therefore, Metrnl deficiency deteriorated ulcerative colitis at least partially through inhibition of autophagy via the AMPK-mTOR-p70S6K pathway, suggesting that Metrnl is a therapeutic target for ulcerative colitis.

2.
Biomolecules ; 9(11)2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31690058

RESUMO

In the current study, corn steep liquor (CSL) is evaluated as an ideal raw agro-material for efficient lipid and docosahexaenoic acid DHA production by Aurantiochytrium sp. Low CSL level in medium (nitrogen deficiency) stimulated the biosynthesis of lipids and DHA while inhibiting cellular growth. The transcriptomic profiles of the Aurantiochytrium sp. cells are analyzed and compared when cultured under high (H group), normal (N group), and low (L group) levels of CSL in the medium. The discriminated transcriptomic profiles from the three groups indicates that changes in CSL level in medium result in a global change in transcriptome of Aurantiochytrium sp. The overall de novo assembly of cDNA sequence data generated 61,163 unigenes, and 18,129 of them were annotated in at least one database. A total of 5105 differently expressed (DE) genes were found in the N group versus the H group, with 2218 downregulated and 2887 upregulated. A total of 3625 DE genes were found in the N group versus the L group, with 1904 downregulated and 1721 upregulated. The analysis and categorization of the DE genes indicates that the regulation mechanism of CSL involved in the perception and transduction of the limited nitrogen signal, the interactions between the transcription factors (TFs) and multiple downstream genes, and the variations in downstream genes and metabolites, in sequence, are illuminated for the first time in the current study.

3.
World J Gastroenterol ; 25(36): 5515-5529, 2019 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-31576097

RESUMO

BACKGROUND: Researchers have investigated the diagnostic value of protein induced by vitamin K absence or antagonist II (PIVKA-II) and alpha-fetoprotein (AFP) in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), and obtained abundant clinical diagnostic data. However, PIVKA-II and AFP have unsatisfactory specificity and sensitivity in the diagnosis of early-stage HBV-related HCC. Gamma-glutamyltransferase (γ-GT) and aspartate aminotransferase (AST) are common biomarkers for evaluating liver function, and we hypothesized that the γ-GT/AST ratio in combination with PIVKA-II and AFP would improve the diagnosis of early-stage HBV-related HCC. AIM: To evaluate the diagnostic value of γ-GT/AST ratio alone or in combination with PIVKA-II and AFP in HBV-related HCC. METHODS: Serum levels of γ-GT, AST, PIVKA-II, and AFP were detected and analysed in 176 patients with HBV-related HCC and in 359 patients with chronic hepatitis B. According to tumour size and serum level of HBV DNA, HBV-related HCC patients were divided into the following categories: Early-stage HCC patients, HCC patients, HBV DNA positive (HBV DNA+) HCC patients, and HBV DNA negative (HBV DNA-) HCC patients. Receiver-operating characteristic (ROC) curves were used to analyse and compare the diagnostic value of the single and combined detection of various biomarkers in different types of HBV-related HCC. RESULTS: Tumour size was positively correlated with serum levels of PIVKA-II and AFP in HCC patients (r = 0.529, a P < 0.001 and r = 0.270, b P < 0.001, respectively), but there was no correlation between tumour size and the γ-GT/AST ratio (r = 0.073, P = 0.336). The areas under the receiver-operating characteristic curves (AUROCs) of the γ-GT/AST ratio in early-stage HCC patients, HBV DNA+ HCC patients and HBV DNA- HCC patients were not significantly different from that in the total HCC patients (0.754, 0.802, and 0.705 vs 0.779, respectively; P > 0.05). When PIVKA-II was combined with the γ-GT/AST ratio in the diagnosis of early-stage HCC, HCC, and HBV DNA+ HCC, the AUROCs of PIVKA-II increased, with values of 0.857 vs 0.835, 0.925 vs 0.913, and 0.958 vs 0.954, respectively. When AFP was combined with the γ-GT/AST ratio in the diagnosis of early-stage HCC, HCC, HBV DNA+ HCC, and HBV DNA- HCC, the AUROCs of AFP increased, with values of 0.757 vs 0.621, 0.837 vs 0.744, 0.868 vs 0.757, and 0.840 vs 0.828, respectively. CONCLUSION: The γ-GT/AST ratio may be better than PIVKA-II and AFP in the diagnosis of early-stage HBV-related HCC, and its combination with PIVKA-II and AFP can improve the diagnostic value for HBV-related HCC.


Assuntos
Aspartato Aminotransferases/sangue , Biomarcadores/sangue , Carcinoma Hepatocelular/diagnóstico , Hepatite B Crônica/complicações , Neoplasias Hepáticas/diagnóstico , Precursores de Proteínas/sangue , alfa-Fetoproteínas/análise , gama-Glutamiltransferase/sangue , Adulto , Carcinoma Hepatocelular/sangue , Carcinoma Hepatocelular/virologia , Feminino , Vírus da Hepatite B/isolamento & purificação , Hepatite B Crônica/patologia , Hepatite B Crônica/virologia , Humanos , Fígado/patologia , Neoplasias Hepáticas/sangue , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade , Protrombina , Curva ROC , Estudos Retrospectivos
4.
Front Physiol ; 10: 1109, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31551804

RESUMO

Background: Coronary heart disease (CHD) remains highly prevalent and is one of the largest causes of death worldwide. Blood stasis syndrome (BSS) is the main syndrome associated with CHD. However, the underlying biological basis of BSS with CHD is not yet been fully understood. Materials and Methods: We proposed a metabolomics method based on 1H-NMR and random forest (RF) models to elucidate the underlying biological basis of BSS with CHD. Firstly, 58 cases of CHD patients, including 27 BSS and 31 phlegm syndrome (PS), and 26 volunteers were recruited from Xiangya Hospital affiliated to Central South University. A 1 mL venous blood sample was collected for NMR analysis. Secondly, principal component analysis (PCA), partial least squares discrimination analysis (PLS-DA) and RF was applied to observe the classification of each group, respectively. Finally, RF and multidimensional scaling (MDS) were utilized to discover the plasma potential biomarkers in CHD patients and CHD-BSS patients. Results: The models constructed by RF could visually discriminate BSS from PS in CHD patients. Simultaneously, we obtained 12 characteristic metabolites, including lysine, glutamine, taurine, tyrosine, phenylalanine, histidine, lipid, citrate, choline, lactate, α-glucose, ß-glucose related to the CHD patients, and Choline, ß-glucose, α-glucose and tyrosine were considered as potential biomarkers of CHD-BSS. Conclusion: The combining of 1H-NMR profiling with RF models was a useful approach to analyze complex metabolomics data (should be deleted). Choline, ß-glucose, α-glucose and tyrosine were considered as potential biomarkers of CHD-BSS.

5.
Environ Sci Pollut Res Int ; 26(28): 29003-29011, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31388952

RESUMO

Cadmium (Cd) re-mobilize by phosphate-solubilizing bacteria (PSB) from immobilization contaminated soil has drawn great attention due to its serious threat to human health through food chain. However, Cd binding with weathered coal (WC), an effective Cd immobilization material, will be re-mobilized by PSB or not is still unclear. In this study, the soil and sand pots with Cd were respectively mixed with the weight fractions of 0‰, 2‰, and 3‰ WC, inoculated with or without PSB, and planted with Amaranthus mangostanus L. The experimental results indicated that: (i) Cd in soil was transformed into organic fraction with WC, which has been led to the Cd accumulation concentrations in roots and shoots reduced by 38.8% and 20.5%, respectively; (ii) PSB could promote the concentration of exchangeable-Cd fraction and soil Cd uptake by amaranth in all treatments; and (iii) WC application in sand pot respectively reduced the Cd accumulation by 47.5% in roots and 24.1% in shoots, but PSB inoculation showed no significant effect on Cd accumulation in plants under WC application. SEM, zeta potential, and FT-IR results showed that PSB inoculation after Cd immobilized by WC had no influence on the microstructure, amount of negative charge, type, and content of functional groups in WC, indicating that organic fraction Cd in WC was not re-mobilized by PSB. Therefore, the application of WC in contaminated soil was conducive to transforming Cd in organic-bound forms and intensifying Cd immobilization effects.


Assuntos
Amaranthus/metabolismo , Bactérias/metabolismo , Cádmio/análise , Carvão Mineral , Fosfatos/química , Solo/química , Poluição Ambiental , Raízes de Plantas/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier , Tempo (Meteorologia)
6.
J Environ Manage ; 244: 453-461, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31154108

RESUMO

Biogas slurry (BS) is a main byproduct of biogas production that is commonly used for agricultural irrigation because of its abundant nutrients and microelements. However, direct application of BS may cause quality decline and nitrate and heavy metal accumulation in crops. To address this issue, a microalgae culture experiment and an irrigation experiment were performed to evaluate the removal efficiencies of nutrients and heavy metals from diluted BS by microalgae Scenedesmus sp. and to investigate the effects of irrigation with microalgae-treated BS (MBS-25, MBS-50, MBS-75, and MBS-100) on nutritional quality, oxidation resistance, and nitrate and heavy metal residues in Chinese cabbage. After 8 days of continuous culture, a ratio of 1/1 for BS/tap water mixture (BS-50) was the optimal proportion for microalgal growth (3.73 g dry cell L-1) and efficient removal of total nitrogen (86.1%), total phosphorus (94.3%), COD (87.5%), Cr (50%), Pb (60.7%), and Cd (59.7%). The pH in MBS-50 medium recovered to the highest level in a shorter period of time and accelerated the gas stripping of ammonia nitrogen and the formation of insoluble phosphate and metals, which partly contributed to the high removal efficiencies. MBS irrigation significantly promoted crop growth; improved nutritional quality, edible taste, and oxidation resistance; and reduced nitrate and heavy metal residues in Chinese cabbage at a large scale. Therefore, microalgae culture was beneficial to reduce negative impacts of BS irrigation in crop growth and agricultural product safety. This study may provide a theoretical basis for the safe utilization of BS waste in agricultural irrigation.


Assuntos
Brassica , Metais Pesados , Microalgas , Biocombustíveis , Valor Nutritivo
7.
Medicine (Baltimore) ; 98(21): e15591, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31124935

RESUMO

RATIONALE: The aim of the present study was to assess the efficacy and safety of percutaneous cannulated screw (PCS) implantation assisted by screw view model of navigation (SVMN) to treat femoral neck fracture (FNF). PATIENT CONCERNS: A 42-year-old male patient suffered from a high falling injury, causing pain, swelling, deformity, and limited mobility on his right hip. DIAGNOSES: He was diagnosed with Garden type I of FNF. INTERVENTIONS: PCS implantation assisted by SVMN was used to treat fracture of femoral neck in this patient. OUTCOMES: The follow up lasted for 48 months. A total of 3 screws were inserted into femoral neck, all exhibiting excellent position. The mean screw deviation was 0.43° and 5.73° of femoral neck-shaft and anteversion angle, respectively. The guide wire drilling attempt of each screw was one-time. The fluoroscopic time lasted 6.3 minutes, the Harris hip scores improved from 67 to 88, and the blood loss was 35 mL. It took 11.7 minutes for designing the screws, 13.9 minutes for implanting the guide wires, and 37.3 minutes for placing the screws. No clinical complications were found during 48-month follow-up visit, including head penetration, implant failure, fracture nonunion, and femoral head osteonecrosis. LESSONS: The study revealed that SVMN is conducive to the PCS insertion for FNF. Our lesson is that the FNF must be well reduction before SVMN assisted PCS placement.


Assuntos
Parafusos Ósseos , Fraturas do Colo Femoral/cirurgia , Fixação Interna de Fraturas/instrumentação , Neuronavegação/métodos , Adulto , Cânula , Colo do Fêmur/lesões , Colo do Fêmur/cirurgia , Fixação Interna de Fraturas/métodos , Consolidação da Fratura , Humanos , Masculino , Resultado do Tratamento
8.
Huan Jing Ke Xue ; 40(4): 1990-1998, 2019 Apr 08.
Artigo em Chinês | MEDLINE | ID: mdl-31087946

RESUMO

Volatile organic compounds (VOCs) and odors, which pose potential hazards to human health and the ecosystem, are two of the most important pollutants emitted from the pharmaceutical fermentation industry. Currently, basic research on the characteristics of the pollution and effective prevention technology for VOCs and odors emitted from the pharmaceutical fermentation industry are limited. Specifically, the pharmaceutical fermentation industry lacks adequate theoretical guidance on the supervision and control of VOCs and odors, and some companies even face relocations. Using the pharmaceutical fermentation industry as the study object, the pollution characteristics of VOCs and odors emitted from different production workshops, sewage treatment stations, and the disposal of pharmaceutical residues were assessed. Based on the studies above, the progress of research into representative control technologies were also reviewed systematically. For VOCs and odors control in the pharmaceutical fermentation industry, four suggestions for future research were proposed:① The production processes should be optimized, and the discharge of pollution should be reduced throughout the entire processes; ② Basic research should be carried out on the pollution characteristics of the VOCs and odors emitted from the pharmaceutical fermentation industry, and a rapid and effective method to trace the sources of VOCs and odors should be established; ③ A comprehensive evaluation of control technologies should be conducted, taking cost and efficiency into account; ④ Emission standards and technical orders for VOCs and odors in the pharmaceutical fermentation industry should be formulated and implemented immediately.


Assuntos
Poluentes Atmosféricos/análise , Poluição do Ar/prevenção & controle , Indústria Farmacêutica , Fermentação , Odorantes/prevenção & controle , Compostos Orgânicos Voláteis/análise
9.
Molecules ; 24(8)2019 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-31013676

RESUMO

The traditional biochemical methods for analyzing cellular composition of oleaginous microorganisms are time-consuming, polluting, and expensive. In the present study, an FT-IR method was used to analyze the cellular composition of the marine oleaginous protist Aurantiochytrium sp. during various research processes, such as strains screening, medium optimization, and fermentation, and was evaluated as a green, low-cost, high throughput, and accurate method compared with the traditional methods. A total of 109 Aurantiochytrium sp. strains were screened for lipid and carbohydrate production and the best results were found for the strains No. 6 and No. 32. The yields and productivities could reach up to 47.2 g/L and 0.72 g/L/h for lipid, 21.6 g/L and 0.33 g/L/h for docosahexaenoic acid (DHA) in the strain No. 6, and 15.4 g/L and 0.18 g/L/h for carbohydrate in the strain No. 32, under the optimal conditions, respectively. These results confirmed potentials of the two Aurantiochytrium sp. strains for lipid, DHA, and carbohydrate productions at industrial scales. The FT-IR method in this study will facilitate research on the oleaginous Aurantiochytrium sp., and the obtained two strains for lipid and carbohydrate productions will provide the foundations for their applications in medical, food, and feed industries.


Assuntos
Carboidratos/biossíntese , Ácidos Docosa-Hexaenoicos/biossíntese , Estramenópilas/metabolismo , Carboidratos/análise , Ácidos Docosa-Hexaenoicos/análise , Espectroscopia de Infravermelho com Transformada de Fourier , Estramenópilas/química
10.
Phys Rev Lett ; 122(9): 090501, 2019 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-30932542

RESUMO

We provide the first example of a symmetry protected quantum phase that has universal computational power. This two-dimensional phase is protected by one-dimensional linelike symmetries that can be understood in terms of the local symmetries of a tensor network. These local symmetries imply that every ground state in the phase is a universal resource for measurement-based quantum computation.

11.
Clin Chim Acta ; 495: 77-81, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30928572

RESUMO

OBJECTIVE: To produce 60-kDa recombinant Sjoren's syndrome antigen A (SSA-60) by gene engineering and establish and evaluate the performance of a magnetic microparticle chemiluminescence quantitative method for detecting anti-SSA-60 antibody in sera. METHODS: Recombinant antigen was prepared by gene recombination technology and purified by high affinity Ni2+ resin. The immunogenicity of the recombinant antigen was verified in immunized BALB/c female mice, and the immune reactivity of our recombinant antigen was assessed using the enzyme-linked immunosorbent assay (ELISA) method. With this recombinant antigen, a specific magnetic microparticles chemiluminescence assay (MMC assay) was developed and performed using various parameters. RESULTS: The inner-group difference among high, medium and low density sera mixtures was 7.65%, 2.24%, and 2.47%, respectively, and the inter-group precision rate was 8.25%, 6.26%, and 4.87%, respectively, using the MMC assay. The low detection limit was 1.36 relative unit per milliliter (Ru/mL), and the quantitative limit was 4.48 Ru/mL. The linear range of this method was 2-400 Ru/mL, which is wider than that of ELISA. The standard error (SE) of the differences was 31.3 between the two methods. Good correlation (y = 1.04x - 7.86, R2 = 0.979, P < 0.05) and high agreement (Kappa = 0.95) were noted between the two methods. CONCLUSIONS: These data show that the MMC assay provides high sensitivity and specificity and a wider linear range in anti-SSA-60 aab detection and fairly good agreement and correlation between the MMC assay and ELISA. The MMC assay has potential in rapid, high-throughput, quantitative and automated autoimmune antibody testing.


Assuntos
Autoanticorpos/sangue , Autoantígenos/imunologia , Medições Luminescentes/métodos , Imãs/química , Microesferas , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Autoanticorpos/imunologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
12.
J Agric Food Chem ; 67(19): 5560-5570, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30901205

RESUMO

The phytohormone 6-benzylaminopurine (6-BAP) significantly improves lipid synthesis of oleaginous microorganisms with the great potential applied in lipid production. In the current study, the lipid and DHA productions in oleaginous Aurantiochytrium sp. were found to be improved by 48.7% and 55.3%, respectively, induced by 6-BAP treatments. Then, using high-throughput RNA-seq technology, the overall de novo assembly of the cDNA sequence data generated 53871 unigenes, and 15902 of these were annotated in at least one database. The comparative transcriptomic profiles of cells with and without 6-BAP treatments revealed that a total of 717 were differently expressed genes (DE), with 472 upregulated and 245 downregulated. Further annotation and categorization indicated that some DE genes were involved in pathways crucial to lipid and DHA productions, such as fatty acid synthesis, central carbon metabolism, transcriptional factor, signal transduction, and mevalonate pathway. A regulation mode of 6-BAP, in turn, perception and transduction of 6-BAP signal, transcription factor, expression regulations of the downstream genes, and metabolic changes, respectively, was put forward for the first time in the present study. This research illuminates the transcriptomic mechanism of phytohormone stimulation of lipid and DHA production in an oleaginous microorganism and provides the potential targets modified using genetic engineering for improving lipid and DHA productivity.


Assuntos
Compostos de Benzil/farmacologia , Ácidos Docosa-Hexaenoicos/biossíntese , Metabolismo dos Lipídeos/efeitos dos fármacos , Reguladores de Crescimento de Planta/farmacologia , Purinas/farmacologia , Estramenópilas/efeitos dos fármacos , Estramenópilas/genética , Compostos de Benzil/química , Perfilação da Expressão Gênica , Regulação da Expressão Gênica/efeitos dos fármacos , Reguladores de Crescimento de Planta/química , Purinas/química , Estramenópilas/metabolismo
13.
Ther Adv Endocrinol Metab ; 10: 2042018818821296, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30728939

RESUMO

The laminin subunit alpha 2 (LAMA2) gene encodes an alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). In the current study, we investigated the relationship between LAMA2 promoter methylation status and the invasiveness of clinically nonfunctioning pituitary adenomas (PitNETs). Specimens from patients with nonfunctioning PitNET were classified into three groups according to preoperative computed tomography (CT)/magnetic resonance imaging findings: a normal group (n = 6), non-invasive group (n = 11) and invasive group (n = 6). LAMA2 expression was assessed using quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting, and the methylation status of the LAMA2 promoter region was observed using sodium bisulfite sequencing. Furthermore, 5-aza-2-deoxycytidine was used to explore the relationship between decreased LAMA expression and methylation in PitNET cells. According to the RT-qPCR and western blotting results, LAMA2 expression was downregulated in invasive PitNET, while the methylation of the LAMA2 promoter was increased. Methylation of the LAMA2 promoter decreased the expression of LAMA2. Thus, changes in LAMA2 expression due to promoter methylation were inversely correlated with the invasiveness of PitNET and the protein functions as a tumor suppressor. In addition, overexpression and demethylation of LAMA2 suppressed the invasion of PitNET cells, partially by exerting effects on the PTEN-PI3K/AKT signaling pathway and matrix metalloproteinase-9 (MMP-9). Furthermore, a xenograft model was also generated, and LAMA2 overexpression significantly suppressed tumor growth in vivo. Thus, LAMA2 expression and methylation patterns might be used as biomarkers to predict the prognosis of patients with PitNET.

14.
Huan Jing Ke Xue ; 40(1): 263-272, 2019 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-30628283

RESUMO

To improve the coagulation performances, new composite coagulants were used to treat different water samples. The results indicate that Ca2+ has no significant effects on the removal efficiency for turbidity in the kaolin system. The residual aluminum decreased from 0.15 mg·L-1 to 0.10 mg·L-1 (AlCl3 was used as coagulant and the coagulant dosage was 0.10 mmol·L-1). The presence of Ca2+ led to the decrease of the amount of negative charges in the HA system and the residual aluminum decreased due to the decrease of the complexation between the HA molecules and Al-based coagulants. When the raw water contained BSA molecules and the coagulant dosage was 0.16 mmol·L-1, the flocs formed by PACl with Ca2+ were larger (~50 µm) than the flocs generated by PACl and the settleability also improved. Under alkaline conditions (pH=8.5), the DOC concentration decreased after coagulation process by~0.2-0.6 mg·L-1 and the residual aluminum decreased by~0.4-0.7 mg·L-1 using composite coagulants. Under acidic conditions (pH=5.5), the concentrations of DOC and residual aluminum did not significantly differ.

15.
Oncol Lett ; 17(2): 2057-2062, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30675272

RESUMO

The biological features of pancreatic cancer and the associated hypoxic environment around the cancer cells often lead to resistance to radiotherapy and chemotherapy. The present study was performed in order to explore the effect pancreatic stellate cells (PSCs) have on the proliferation of pancreatic cancer cells. In the present study, PSCs from human pancreatic cancer tissues were isolated, and the PSCs markers α-smooth muscle actin and desmin were overexpressed in the cytoplasm of PSCs. An MTT assay revealed that PSCs promoted the viability of pancreatic cancer cells. However, the viability of pancreatic cancer cells promoted by PSCs was partially blocked by SB525334. Cellular invasion analysis demonstrated that PSCs promoted the invasion ability of pancreatic cancer cells. An apoptosis assay indicated that PSCs decreased the level of apoptosis induced by gemcitabine. In vivo experiments consisting of mice bearing MIA-PaCa-2 and PSCs demonstrated an increase in the rate of tumor growth compared with MIA-PaCA-2 alone, whereas SB525334 may delay the tumor progression induced by PSCs. The present findings indicated that PSCs promoted the viability and invasion of pancreatic cancer cells, and decreased the apoptosis of pancreatic cancer cells induced by gemcitabine.

16.
Medicine (Baltimore) ; 97(49): e13316, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30544391

RESUMO

RATIONALE: The purpose of this study was to estimate the efficacy and safety of percutaneous periacetabular screw (PPS) insertion assisted by screw view model of navigation (SVMN) to treat fracture of acetabulum. PATIENT CONCERNS: A 61-year-old male patient was injured in a motorcycle accident, which caused pain, swelling, deformity and limited mobility on his right hip. DIAGNOSES: He was diagnosed with fracture of acetabulum. INTERVENTIONS: We used PPS insertion assisted by SVMN to treat fracture of acetabulum in this patient. OUTCOMES: The follow up lasted 24 months. Totally 2 screws were inserted into anterior and posterior column of acetabulum respectively and both of them displayed grade 0. Compared with the preoperative gap and step of fracture displacement, the postoperative ones were significantly reduced. It took 11.7 minutes for designing the screws, 6.7 minutes for implanting the guide wire, and 45.5 minutes for placing the screws. Intraoperative blood loss was 29 mL and total fluoroscopic time was 4.1 minutes. No clinical complications such as nerve vascular injury, infection and screw loosening were found after the operation. LESSONS: The study indicated that SVMN is favorable to the PPS insertion for acetabular fracture. Our lesson is that the relative position between the acetabular and the patient tracker must be static to ensure the accuracy of the entire system throughout the operation.


Assuntos
Acetábulo/lesões , Acetábulo/cirurgia , Parafusos Ósseos , Fixação Interna de Fraturas/métodos , Fraturas Ósseas/cirurgia , Acetábulo/diagnóstico por imagem , Fixação Interna de Fraturas/efeitos adversos , Fraturas Ósseas/diagnóstico por imagem , Humanos , Masculino , Pessoa de Meia-Idade
17.
ACS Appl Mater Interfaces ; 10(41): 35154-35163, 2018 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-30277387

RESUMO

Solar vapor generation has attracted tremendous attention as one of the most efficient ways of utilizing solar energy. It is highly desirable to develop low-cost, eco-friendly, and high-efficiency solar absorbers for practical applications of solar vapor generation. Herein, a three-dimensional plasmonic covellite CuS hierarchical nanostructure has been synthesized as the light-absorbing material via a facile one-pot hydrothermal method for structurally integrated solar absorbers with microporous poly(vinylidene fluoride) membrane (PVDFM) as the supporting material. A broadband and highly efficient light absorption has been achieved in the wavelength of 300-2500 nm, along with high water evaporation efficiencies of 90.4 ± 1.1 and 93.3 ± 2.0% under 1 and 4 sun irradiation, respectively. Meanwhile, stable performance has been demonstrated for over 20 consecutive runs without much performance degradation. To the best of our knowledge, this is the highest performance among the copper sulfide-based solar absorbers. With the additional features of low-cost and convenient fabrication, this plasmonic solar absorber exhibits a tremendous potential for practical solar vapor generation.

18.
Huan Jing Ke Xue ; 39(8): 3704-3712, 2018 Aug 08.
Artigo em Chinês | MEDLINE | ID: mdl-29998677

RESUMO

In this study, the effect of Cr(Ⅵ) in industrial wastewater on the coagulation of different aluminum forms under the influence of different particle concentrations was investigated. The coagulation efficiency was determined by examining the removal rate of turbidity, residual aluminum, and residual chromium and by using the residual pH value, zeta potential, and floc properties of the coagulation to reveal the coagulation mechanism of Cr(Ⅵ) in water under different turbidities. The experimental results show that under low turbidity conditions, Cr(Ⅵ) greatly influences the coagulation process of highly polymerized Alb, yet has no obvious effect on oligomeric Ala. Under high turbidity conditions, particulate matter will adsorb part of the Cr(Ⅵ) in high turbidity water, thus reducing its interaction with Alb. The main role Alb plays in coagulation is charge neutralization. It plays the same role in the stability of the particles and floc regeneration. The main role of Alc formed by hydrolysis of Ala is bridging effects and sweep flocculation, which plays an important role in floc production and strength factor. At the same time, the existence of Cr(Ⅵ) enhances the strength factor of Al13 flocs, but the consumption of some of the positive charge will lead to a reduction in the floc recovery factor.

19.
Phys Rev Lett ; 120(20): 200503, 2018 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-29864353

RESUMO

Topological qubits based on SU(N)-symmetric valence-bond solid models are constructed. A logical topological qubit is the ground subspace with twofold degeneracy, which is due to the spontaneous breaking of a global parity symmetry. A logical Z rotation by an angle 2π/N, for any integer N>2, is provided by a global twist operation, which is of a topological nature and protected by the energy gap. A general concatenation scheme with standard quantum error-correction codes is also proposed, which can lead to better codes. Generic error-correction properties of symmetry-protected topological order are also demonstrated.

20.
Artigo em Inglês | MEDLINE | ID: mdl-29707037

RESUMO

Background: The traditional Chinese medicine formula Jiu Wei Zhen Xin Granula (JWZXG) is prescribed to treat generalized anxiety disorder (GAD) in China. This study was to assess the efficacy and safety of JWZXG in patients with GAD. Method: Data were pooled from 14 randomized controlled trials involving the assessment of mean changes of Hamilton Anxiety Rating Scale (HAMA) total scores, response rates, adverse event rates, quality, publication bias, and risk of bias. Results: Pooled analysis showed no significant difference in response rate (risk ratio 1.01, 95% CI [0.93-1.08]; Z test = 0.17, P = 0.86) and no significant difference between JWZXG group and azapirones group (RR 0.69, 95% CI [0.45, 1.06]; Z test = 1.69, P = 0.09) in rate of adverse events. Though no difference exists between JWZXG group and azapirones group in HAMA total score from baseline, JWZXG group was inferior to selective serotonin reuptake inhibitors (SSRIs) group (WMD -0.93, 95% CI [-1.64, -0.23]; Z test = 2.6, P = 0.009) which had more adverse events than JWZXG group (RR 0.64, 95% CI [0.46, 0.89]; Z test = 2.63, P = 0.009). Conclusions: This meta-analysis preliminarily suggests that JWZXG is as effective as azapirones, though having the same possibility of suffering AEs. JWZXG was inferior to SSRIs but causes fewer AEs in the treatment of GAD.

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