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1.
Chemosphere ; 239: 124707, 2019 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-31479912

RESUMO

Site soils with persistent cyanide compounds (primarily iron-cyanide complex) pose potential hazards to the environment and require remediation before redevelopment. This study evaluated the possibility of thermal treatment on remediation of cyanide-contaminated soils via batch heating experiments spanning a wide temperature range (200-500 °C). The change with operation variables of total cyanide and some reaction intermediates (e.g. CN-) was analyzed in order to elucidate the optimal variables that guarantee cyanide removal while generating no hazardous byproducts. Temperature, heating time and cyanide species have been found to be important parameters influencing removal/destruction of cyanide in soils. For soils bearing K3[Fe(CN)6] and K4[Fe(CN)6], a removal efficiency of >99.9% can be obtained with temperatures over 350 °C at 1 h, while for samples bearing Fe4[Fe(CN)6]3, a higher temperature (>450 °C) is needed to obtain an equivalent efficiency. During heating, the iron-cyanide complexes decomposed, releasing highly toxic free cyanides, which will subsequently be oxidized. However, a small percentage of free cyanide can always be detected as a result of incomplete oxidation, thus caution should be taken to minimize the accumulation of free cyanide during thermal treatment.

2.
Curr Med Chem ; 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31480994

RESUMO

Drug repositioning is an important area of biomedical research. The drug repositioning studies have shifted to computational approaches. Large-scale perturbation databases, such as the Connectivity Map and the Library of Integrated Network-Based Cellular Signatures, contain a number of chemical-induced gene expression profiles and provide great opportunities for computational biology and drug repositioning. One reason is that the profiles provided by the Connectivity Map and the Library of Integrated Network-Based Cellular Signatures databases show an overall view of biological mechanism in drugs, diseases and genes. In this article, we provide a review of the two databases and their recent applications in drug repositioning.

3.
PLoS One ; 14(9): e0222003, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31483841

RESUMO

In this study, a new type of N2-inhibitor-water mist (NIWM) technology was proposed to resolve the problem of fire prevention and extinguishing in the goaf of coal mine. The corresponding equipment was designed and manufactured. Under the condition that both gas pressure and liquid pressure were 0.5-2MPa, the NIWM equipment produced the water mist with Sauter mean diameter (SMD) range of 166-265µm. The experimental results of the operating parameters of NIWM equipment were in agreement with the theoretical derivation. The theory of two-phase flow atomisation can be used as theoretical guide for this technology. After that, on the basis of the NIWM equipment, the experiments of inhibiting low temperature (30-100°C) oxidation and extinguishing high temperature combustion of large dosage of coal sample were carried out. Water mist with SMD = 188µm had good diffusivity in the container. The inhibiting effect of N2-inhibitor-water mist on low temperature oxidation of coal was obviously greater than that of single material. N2-water mist extinguished the burning coal completely in 20 minutes. The addition of water mist solved the shortcoming of poor cooling effect of N2. In different stages of coal-oxygen reaction, N2, inhibitor and water mist play very different role in controlling the process of coal-oxygen reaction, which was not simple accumulation of the three. The combination of N2, inhibitor and water mist should be determined according to the state of the coal mine goaf fire. On the basis of the research conclusions, the onsite arrangement diagram of the NIWM fire prevention and extinguishing equipment in the goaf was designed. The research results proved the feasibility and effectiveness of this technology, and it is of great significance to the prevention and control of coal spontaneous combustion in goaf.

4.
Int Heart J ; 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31484864

RESUMO

Occurring in about 1% of all live births, congenital heart defects (CHDs) represent the most frequent type of developmental abnormality and account for remarkably increased infant morbidity and mortality. Aggregating studies demonstrate that genetic components have a key role in the occurrence of CHDs. Nevertheless, due to pronounced genetic heterogeneity, the genetic causes of CHDs remain unclear in most patients. In this research, 114 unrelated patients affected with CHDs and 218 unrelated individuals without CHDs served as controls were recruited. The coding regions and splicing donors/acceptors of the ISL1 gene, which codes for a transcription factor required for proper cardiovascular development, were screened for mutations by sequencing in all study participants. The functional characteristics of an identified ISL1 mutation were delineated with a dual-luciferase reporter assay system. As a result, a new heterozygous ISL1 mutation, NM_002202.2: c.225C>G; p. (Tyr75*), was discovered in an index patient with double outlet right ventricle and ventricular septal defect. Analysis of the proband's family unveiled that the mutation co-segregated with the CHD phenotype. The nonsense mutation was absent in the 436 control chromosomes. Biological analysis showed that the mutant ISL1 protein had no transcriptional activity. Furthermore, the mutation nullified the synergistic activation between ISL1 and TBX20, another CHD-associated transcription factor. This research for the first time links an ISL1 loss-of-function mutation to double outlet right ventricle in humans, which adds insight to the molecular pathogenesis underpinning CHDs, suggesting potential implications for timely personalized management of CHD patients.

5.
Medicine (Baltimore) ; 98(36): e17073, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31490408

RESUMO

Extremely severe scoliosis patients, especially main thoracic Cobb' s angle >150°, often have severe thoracic deformity and pulmonary dysfunction, even the scoliosis is reduced by halo-pelvic traction, the improvement of pulmonary function is not satisfactory, the risk of spinal osteotomy in the next stage is still very high and left with obvious thoracic deformity. How to further improve the pulmonary function and appearance of these patients is a difficult problem to be solved.Twenty extremely severe scoliosis patients with severe pulmonary dysfunction who underwent concave-side thoracoplasty in our hospital from September 2014 to September 2017 were included, data of thoracic volume and pulmonary function were collected before and after operation. The pulmonary function value reported was predicted forced vital capacity (FVC%), T-test was used to analyze the changes of the data by the statistical software SPSS21.0.The 20 patient's averaged Cobb's angle of main thoracic was 163° ± 8° at admission and all of them with severe pulmonary dysfunction before concave-side thracoplasty. After operation, the thoracic volume of patients increased by 500.9 ±â€Š222.9 mL, FVC% increased by 8.9% ±â€Š7.5%. Both the difference has statistical significance (P < .01).Concave-side thoracoplasty based on the halo-pelvic traction cannot only enlarge the volume of the concave thoracic cavity, lighten the compression of lung and further improve the pulmonary function of extremely severe scoliosis, but also can strengthen the correction of scoliosis and spinal rotation. Therefore, it is a safe and effective surgical approach.

6.
BMC Musculoskelet Disord ; 20(1): 411, 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31488123

RESUMO

BACKGROUND: Developmental factors were assumed to be the key factors that influenced the morphology of femoral trochlea. This study investigated the effects of insufficient patellar stress after birth on the morphological development of the femoral trochlea. Effects of insufficient patellar stress on femoral trochlea were investigated using surgical induced patellectomy and patellar dislocation in growing rat model. METHODS: In this study, two experimental groups and one sham group (SG) were established. Thirty-six Wistar rats (female, 28 days of age) were randomly assigned to three groups. The patellectomy group (PG), rats underwent the patellectomy in this group. The dislocation group (DG), rats underwent the surgery-induced patellar dislocation. Histological staining (Safranin-O and fast green), Micro-computed tomographic (Micro-CT) analysis in two experimental endpoints (3, 12 weeks postoperatively) were selected to evaluate morphological changes of the femoral trochlea. RESULTS: Articular cartilage on the trochlear sulcus was remodeled at 3 weeks after the surgery, and degenerated at 12 weeks through the histological staining. The femoral trochlear angle (FTA) did not show a significant difference at 3 week between the experimental groups and the sham group (PG vs SG P = 0.38, DG vs SG p = 0.05), but the FTA was significantly increased in experimental groups at 12 weeks(PG vs SG P = 0.001, DG vs SG p = 0.005). The Bone volume density (BV/TV), Trabecular number (Tb.N) under the trochlea groove were significantly reduced at 3 weeks postoperatively in the experimental groups (PG vs SG p = 0.001, DG vs SG p = 0.002). No significant difference was found in BV/TV and Tb. N among the three groups at 12 weeks postoperatively. CONCLUSION: Surgical induced patellectomy and patellar dislocation leads to the dysplastic trochlear sulcus in growing rats. Besides the bone morphology of trochlear sulcus, the articular cartilage and subchondral trabecula under the trochlear sulcus were remodeled early stage after the surgery.

7.
Sci Total Environ ; 690: 1162-1169, 2019 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-31470479

RESUMO

Perfluorinated compounds (PFCs) have been detected at various concentrations in different environment compartments due to their widespread usage. Nowadays, soil environment has become a prominent sink of PFCs from surface runoff and penetration, but few researches have been conducted in the toxicity of PFCs to soil microorganisms. To address the issue, microcalorimetry was applied to investigate the toxicity of six PFCs with different carbon chain length (4, 8, and 10) and functional group (carboxylic and sulfonic) to microbial activities in three Chinese soils varying widely in soil properties. Adsorption of PFCs by soil matrix was a key factor in controlling the toxicity of PFCs to soil microorganisms. The differences of carbon chain length and functional groups of PFCs have different impacts on soil microbial activity while affecting adsorption progress. Particularly, the sulfonic PFCs expressed higher toxicity than the carboxylic. It is also identified that the longer the chain length, the greater the toxicity of PFCs. Soil pH was another relevant factor of soil adsorption, and with the increase of pH, adsorption capability increased. Soil available P, N and K were essential nutrients in soil, and suggested to improve microbial activity under PFCs stress.

8.
Aging (Albany NY) ; 112019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31477637

RESUMO

OBJECTIVES: Autophagy is an evolutionarily conserved intracellular degradation mechanism in which cell constituents are phagocytosed to maintain cellular homeostasis. Forkhead box O 3a (FoxO3a) promotes autophagy to protect cells from environmental stress. Methylated CpG binding protein 2 (MeCP2) is a nuclear protein that binds DNA and represses transcription. However, the mechanism and interplay between FoxO3a and MeCP2 underlying endothelial progenitor cell (EPC) function are not fully understood. RESULTS: In EPCs, MeCP2 overexpression attenuated autophagy and cell functionality, which were reversed by the autophagy activator rapamycin or co-transfection with FoxO3a. FoxO3a promoted cell function, which was reversed by the autophagy inhibitor chloroquine. Following MeCP2 overexpression, MeCP2 was found enriched on the FoxO3a promoter, resulting in promoter hypermethylation and enhanced H3K9 histone modification in nucleosomes of the FoxO3a promoter. CONCLUSIONS: MeCP2 attenuated cell functionality via DNA hypermethylation and histone modification of the FoxO3a promoter to inhibit FoxO3a transcription and autophagy. MATERIALS AND METHODS: EPCs were isolated from human umbilical cord blood and treated with adenoviral vectors containing interference sequences. The effects and mechanism of MeCP2 and FoxO3a were analyzed by utilizing western blotting, cell counting kit-8, transwell plates, Matrigel, matrix adhesion, transmission electron microscopy, and chromatin immunoprecipitation.

9.
Sci Rep ; 9(1): 12661, 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31477771

RESUMO

Cassava (Manihot esculenta) is a major staple food, animal feed and energy crop in the tropics and subtropics. It is one of the most drought-tolerant crops, however, the mechanisms of cassava drought tolerance remain unclear. Abscisic acid (ABA)-responsive element (ABRE)-binding factors (ABFs) are transcription factors that regulate expression of target genes involved in plant tolerance to drought, high salinity, and osmotic stress by binding ABRE cis-elements in the promoter regions of these genes. However, there is little information about ABF genes in cassava. A comprehensive analysis of Manihot esculenta ABFs (MeABFs) described the phylogeny, genome location, cis-acting elements, expression profiles, and regulatory relationship between these factors and Manihot esculenta betaine aldehyde dehydrogenase genes (MeBADHs). Here we conducted genome-wide searches and subsequent molecular cloning to identify seven MeABFs that are distributed unevenly across six chromosomes in cassava. These MeABFs can be clustered into three groups according to their phylogenetic relationships to their Arabidopsis (Arabidopsis thaliana) counterparts. Analysis of the 5'-upstream region of MeABFs revealed putative cis-acting elements related to hormone signaling, stress, light, and circadian clock. MeABF expression profiles displayed clear differences among leaf, stem, root, and tuberous root tissues under non-stress and drought, osmotic, or salt stress conditions. Drought stress in cassava leaves and roots, osmotic stress in tuberous roots, and salt stress in stems induced expression of the highest number of MeABFs showing significantly elevated expression. The glycine betaine (GB) content of cassava leaves also was elevated after drought, osmotic, or salt stress treatments. BADH1 is involved in GB synthesis. We show that MeBADH1 promoter sequences contained ABREs and that MeBADH1 expression correlated with MeABF expression profiles in cassava leaves after the three stress treatments. Taken together, these results suggest that in response to various dehydration stresses, MeABFs in cassava may activate transcriptional expression of MeBADH1 by binding the MeBADH1 promoter that in turn promotes GB biosynthesis and accumulation via an increase in MeBADH1 gene expression levels and MeBADH1 enzymatic activity. These responses protect cells against dehydration stresses by preserving an osmotic balance that enhances cassava tolerance to dehydration stresses.

10.
Dysphagia ; 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31463803

RESUMO

Denture impacted in the esophagus of adults has been a complex foreign body for otolaryngologists. We reviewed clinical characteristics, diagnosis, and treatment of these patients and evaluated computed tomography (CT) scans to identify a better method of dealing with such tricky situations. Twenty-nine patients who underwent rigid esophagoscopy were included in this retrospective study conducted at the University hospital. The patients underwent preoperative tests and examinations, including complete blood count, blood type and coagulation, electrocardiogram, and CT. The commonest symptoms were retrosternal pain, dysphagia, and odynophagia. Duration of the foreign body impacted within 24 h was 65.5%. CT findings revealed that 4 of 24 cases had complications in the upper esophagus, with 3 of the 4 cases in the mid-esophagus and 1 in the lower esophagus. Complications were related to the duration and location of the obstruction (P < 0.05). The location and complications based on CT findings were coherent with rigid esophagoscopy findings. Denture impaction in the esophagus can be fatal. Early intervention is crucial for prognosis. CT is used for diagnosing and guiding doctors in managing. The commonest location of impacted dentures was the upper esophagus with a lower incidence of complications. The incidence of an impacted denture in the mid-esophagus was low but with a high risk of complications. The incidence of an impacted denture in the lower esophagus was rare. Surgery and proper treatment ensure a good prognosis.

11.
Nano Lett ; 2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31433196

RESUMO

Because of its high theoretical volumetric capacity and dendrite-free stripping/plating of Mg, rechargeable magnesium batteries (RMBs) hold great promise for high energy density in consumer electronics. However, the lack of high-energy-density cathodes severely constrains their practical applications. Herein, for the first time, we report that a CuS cathode can fully reversibly work through a displacement reaction in CuS/Mg pouch cells at room temperature and provide a high capacity of ∼400 mA h/g in a MACC electrolyte, corresponding to the gravimetric and volumetric energy density of 608 W h/kg and1042 W h/L, respectively. Even after 80 cycles, CuS/Mg pouch cells can maintain a high capacity of 335 mA h/g. Detailed mechanistic studies reveal that CuS undergoes a displacement reaction route rather than a typical conversion mechanism. This work will provide a guide for more discovery of high-performance cathode candidates for RMBs.

12.
Insect Biochem Mol Biol ; : 103225, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31446032

RESUMO

Negative regulation of the immune signaling pathway involves diverse negative regulators that target different signaling molecules. One of the signaling molecules, DREDD, which activates the NF-κB transcription factor Relish in the IMD pathway, is a homolog of mammalian caspase-8. Some structural related proteins have been identified to regulate the activity of caspase-8 in signaling complex assembly. However, it is unknown in insects whether the IMD pathway undergoes such a down-regulation. In this study, we explored the regulatory role of a newly identified protein BmCaspase-8 like (BmCasp8L) in silkworm, which displays high sequence similarity with the N-terminus of BmDREDD to the IMD pathway, and investigated its mechanism. Domain prediction, phylogenic analysis and gene architecture suggests BmCasp8L acts as a potential inhibitor to BmDREDD. We then found it is highly expressed in the fat body and hemocytes, and suppresses the cleavage of BmRelish and BmIMD mediated by BmDREDD upon PGN stimulation, resulting in deficiency in antimicrobial peptides production. Besides the inhibitory role in the IMD pathway, it also suppresses the BmDREDD-induced apoptosis. By investigating the amyloidal activity of BmCasp8L and its interaction with BmDREDD and BmFADD, we demonstrated that BmCasp8L forms amyloid-like aggregates in vitro as well as in vivo, and it inactivates BmDREDD by blending into the amyloidal speck-like structure formed by BmDREDD and BmFADD that is required for BmDREDD activity. Taken together, our results demonstrate BmCasp8L inhibits the IMD signaling pathway via forming amyloidal aggregates with BmDREDD, suggesting an evolutionarily conserved regulatory mechanism of innate immune signaling pathway.

13.
Behav Brain Res ; 374: 112115, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31369775

RESUMO

Exercise has been considered for the treatment of depression, but the mechanism by which exercise improves depression is still unclear. To clarify the mechanism, rats were randomly divided into the control, chronic unpredictable stress (CUS)/standard and CUS/running groups. The rats in the CUS/running group ran for four weeks. In this study, a sucrose preference test (SPT) was used to evaluate the depression-like symptoms in the rats, and western blot, immunohistochemical and stereological analyses were performed to study the expression of synaptic-related proteins in the hippocampus and the changes in excitatory synapses in each sub-region. The results show that sucrose preference in the CUS/standard group was significantly lower than that in the control group, but in the CUS/running group, sucrose preference was higher than that in the CUS/standard group. Surprisingly, there was no difference in the synaptic-related proteins in the hippocampus among groups. The CUS/standard group exhibited fewer spinophilin+ (Sp+) dendritic spines representing excitatory synapses in CA1, CA3 and dentate gyrus (DG) of the hippocampus than the control group, whereas the CUS/running group exhibited significantly more Sp+ dendritic spines in these regions than the CUS/standard group, indicating that excitatory synapses were reduced in depressed rats and that running exercises could reverse this change. We hypothesize that the changes in the number of excitatory synapses better reflect the changes in depressive symptoms than the level of synaptic proteins and that the effect of exercise on excitatory synapses in the sub-regions of the hippocampus may be an important structural indicator of the improvement of depressive symptoms.

14.
Orthop Surg ; 11(4): 578-585, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31419069

RESUMO

OBJECTIVES: To report the clinical outcomes of combined femoral derotation osteotomy and medial retinaculum plasty for recurrent patellar dislocation in patients with excessive femoral anteversion. METHODS: From January 2015 to March 2018, 20 knees in 20 patients (18 female, 2 male) with a mean age of 21 ± 4.2 years (range, 16 to 28 years) were retrospectively reviewed. All patients had undergone femoral derotation osteotomy and medial retinaculum plasty for recurrent patellar dislocation and excessive femoral anteversion angle (FAA > 25°). CT and X-rays were used to assess the correction of the femoral anteversion angle, the tibia tuberosity-trochlear groove (TT-TG) distance, patellar tilt, and the congruence angle following the combinatory operations. Subjective scores, such as Kujala, International Knee Documentation Committee (IKDC), Tegner, and visual analogue scale (VAS) scores, were used to evaluate knee function preoperatively and postoperatively. RESULTS: No recurrence of patellar dislocation occurred in these patients during an average of 18 months (range, 12 to 23 months) of follow-up. The mean of the FAA was corrected to 15.80° ± 3.58° postoperatively compared with 31.42° ± 4.95° preoperatively (P < 0.001). The TT-TG distance was decreased from 22.17 ± 5.28 mm before surgery to 19.42 ± 4.57 mm after surgery (P = 0.03). The patellar tilt and congruence angle were improved from 30.43° ± 5.30°, 43.30° ± 11.04° to 15.80° ± 3.94°, 16.64° ± 9.98°, respectively (P < 0.001). The Kujala score was improved from 72.4 ± 19.90 before the surgery to 88.2 ± 12.25 after the surgery (P < 0.001). The IKDC score was improved from 70.56 ± 21.44 to 90.78 ± 14.32, and the VAS score was decreased from 4.23 ± 2.11 preoperatively to 1.27 ± 1.08 postoperatively (P < 0.001). No significant difference in Tegner score (5.46 ± 2.49 vs 5.79 ± 1.44) was found before and after the surgery (P = 0.2). Patients younger than 20 years old had lower Kujala (83.46 ± 14.56 vs. 90.84 ± 7.74, P = 0.02) and IKDC (83.49 ± 17.35 vs 92.46 ± 9.28, P = 0.04) scores than those older than 20 years. CONCLUSION: Good knee function, pain relief, and improved patellofemoral congruence were achieved with the combined femoral derotation osteotomy and medial retinaculum plasty. The combined operations serve as an ideal treatment for recurrent patellar dislocation and address the primary risk factors.

15.
FASEB J ; : fj201901089R, 2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31450968

RESUMO

Mumps virus (MuV) has high tropism to the testis and may lead to male infertility. Sertoli cells are the major targets of MuV infection. However, the mechanisms by which MuV infection impairs male fertility and Sertoli cell function remain unclear. The present study elucidated the effect of MuV infection on the blood-testis barrier (BTB). The transepithelial electrical resistance of MuV-infected mouse Sertoli cells was monitored, and the expression of major proteins of the BTB was examined. We demonstrated that MuV infection disrupted the BTB by reducing the levels of occludin and zonula occludens 1. Sertoli cells derived from Tlr2-/- and Tnfa-/- mice were analyzed for mediating MuV-induced impairment. TLR2-mediated TNF-α production by Sertoli cells in response to MuV infection impaired BTB integrity. MuV-impaired BTB was not observed in Tlr2-/- and Tnfa-/- Sertoli cells. Moreover, an inhibitor of TNF-α, pomalidomide, prevents the disruption of BTB in response to MuV infection. FITC-labeled biotin tracing assay confirmed that BTB permeability and spermatogenesis were transiently impaired by MuV infection in vivo. These findings suggest that the disruption of the BTB could be one of the mechanisms underlying MuV-impaired male fertility, in which TNF-α could play a critical role.-Wu, H., Jiang, X., Gao, Y., Liu, W., Wang, F., Gong, M., Chen, R., Yu, X., Zhang, W., Gao, B., Song, C., Han, D. Mumps virus infection disrupts blood-testis barrier through the induction of TNF-α in Sertoli cells.

16.
Stud Health Technol Inform ; 264: 462-466, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31437966

RESUMO

Acute Kidney Injury (AKI) is the most common cause of organ dysfunction in critically ill adults and prior studies have shown AKI is associated with a significant increase of the mortality risk. Early prediction of the mortality risk for AKI patients can help clinical decision makers better understand the patient condition in time and take appropriate actions. However, AKI is a heterogeneous disease and its cause is complex, which makes such predictions a challenging task. In this paper, we investigate machine learning models for predicting the mortality risk of AKI patients who are stratified according to their AKI stages. With this setup we demonstrate the stratified mortality prediction performance of patients with AKI is better than the results obtained on the mixed population.

18.
Photochem Photobiol ; 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31441061

RESUMO

UVA can penetrate dermis and cause functional damage of dermal fibroblasts leading photoaging. Ginseng is a widely used traditional Chinese medicine for skin aging. However, its effects on skin photoaging induced by UVA are not clear. In this study, we isolated ginseng proteins (GP), with molecular weights of 27 kDa and 13 kDa, and found that they alleviated the inhibitory effects of UVA on cell viability and increased percentage of NIH-3T3 fibroblasts in the S phase of cells cycle. GP also improved cell contraction ability, increased the expression and secretion of CoL-I, similar to MAPK phosphorylation inhibitors, and reduced expression and secretion of MMP-1, MMP-2 and MMP-9 as well as the enzyme activities of MMP-2 and MMP-9. They reduced ROS content, DNA damage, and 8-OHdG content, as well as the protein expression of p53, p21, and p16. The levels of p-ERK, p-p38 and p-JNK, p-c-Fos, and p-c-Jun proteins were decreased by GP. Inactivated GP did not inhibit the cellular activity and expression and secretion of CoL-I irradiated by UVA. The results showed that GP can improve cell viability and contractile function by inhibiting DNA damage and collagen degradation to inhibit the photoaging effects of skin dermal cells caused by UVA. This article is protected by copyright. All rights reserved.

19.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 31(7): 903-905, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31441419

RESUMO

OBJECTIVE: To observe the effect of early enteral nutrition (EEN) on nutritional indicators and clinical outcomes in patients with severe heart failure undergoing mechanical ventilation. METHODS: Thirty-four patients with severe heart failure (grade III-IV of cardiac function) and pulmonary infections undergoing mechanical ventilation admitted to intensive care unit (ICU) of Second Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine from May 2017 to April 2018 were enrolled. They were randomly divided into EEN group and delayed enteral nutrition (DEN) group. Both groups were given routine treatment, including mechanical ventilation, improvement of cardiac function, anti-infection, protection of vital organ function, regulation of blood sugar and adjustment of electrolyte and acid-base balance. The patients in EEN group received enteral nutrition (EN) within 48 hours after ICU admission, and in DEN group, EN was started after the patients had spent the early stage of stress and had stable vital signs (48 hours after ICU admission). The changes in serum B-type natriuretic peptide (BNP), C-reactive protein (CRP), nutritional indicators and liver function indicators at ICU admission and 7 days after treatment were compared between the two groups. The time needed for patients to reach EN target, duration of mechanical ventilation, length of ICU stay, and the 28-day mortality were recorded, and complications were observed. RESULTS: There was no significant difference in serum BNP, CRP, nutritional indicators or liver function indicators at ICU admission between the two groups. After treatment for 7 days, BNP and CRP in both groups were decreased significantly as compared with those at ICU admission [BNP (ng/L): 592.1±370.9 vs. 2 517.7±1 163.4 in EEN group, 621.9±418.8 vs. 2 251.5±1 006.8 in DEN group; CRP (mg/L): 46.0±19.6 vs. 59.8±22.5 in EEN group, 40.5±18.8 vs. 61.2±24.6 in DEN group, all P < 0.05], pre-albumin (PA) and transferrin (TF) were significantly increased [PA (g/L): 0.18±0.05 vs. 0.15±0.06 in EEN group, 0.17±0.04 vs. 0.12±0.06 in DEN group; TF (g/L): 1.6±0.4 vs. 1.5±0.4 in EEN group, 1.7±0.5 vs. 1.4±0.5 in DEN group, all P < 0.05]. However, there was no significant difference in the above indicators after treatment between the two groups (all P > 0.05). There was no significant change in liver function after treatment in both groups. The EN treatment was successfully completed in both groups. Some patients developed abdominal distension and diarrhea in varying degrees, which were alleviated by slowing down the infusion rate, supplemented by gastrointestinal motility drugs and intestinal flora adjustment drugs. The time needed to reach EN target in EEN group was significantly earlier than that in DEN group (hours: 42.4±10.2 vs. 53.8±17.1, P < 0.05), the duration of mechanical ventilation (days: 14.2±8.7 vs. 13.4±7.9), the length of ICU stay (days: 17.8±6.7 vs. 18.3±5.6) and 28-day mortality [5.9% (1/17) vs. 11.8% (2/17)] showed no significant difference as compared with those in DEN group (all P > 0.05), and it did not increase the incidence of aspiration pneumonia [23.5% (4/17) vs. 17.7% (3/17), P > 0.05]. CONCLUSIONS: EEN could help to achieve nutritional goals as soon as possible, improve the nutritional status of the body, and provide conditions and basis for further treatment of severe heart failure patients.

20.
Int J Oncol ; 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31432157

RESUMO

Reports on the roles of the secreted trefoil factor (TFF)1 and 3 in colorectal cancer (CRC) and their underlying mechanisms of action in tumorigenesis are not common and are controversial. In the present study, the mRNA expression and promoter methylation of TFF1 and TFF3 in cancer and adjacent normal tissues were investigated, and their association with other clinical factors and patient prognosis were evaluated. Moreover, the association between TFF3 and epithelial­mesenchymal transition (EMT) was explored by overexpressing or inhibiting TFF3 expression. The results revealed that the mRNA level of TFF1 and TFF3 in the cancer tissues was significantly higher than that in the matched adjacent normal tissues (P=0.034 and P=0.007, respectively), and a higher expression of TFF3, but not TFF1, was predominantly associated with clinicopathological factors and a poorer prognosis. No correlation was observed between promoter methylation and the expression of TFF1 or TFF3. The overexpression of TFF3 promoted the proliferation, migration and invasiveness of HT29 cells, and induced an increase in the expression of Twist1, Snail and Vimentin, while causing a decrease in E­cadherin expression. On the contrary, the knockdown of TFF3 resulted in opposite effects in the LoVo cells. On the whole, the findings of this study indicate that TFF3 may be a promising new factor for the estimation of the survival of patients with CRC, and may promote the malignant progression of CRC by activating the EMT process. Therefore, TFF3 may be a future potential therapeutic target for CRC.

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