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1.
Chem Asian J ; 15(2): 319-324, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31825169

RESUMO

Determination of the absolute configuration (AC) of chiral molecules is a key issue in many fields related to chirality such as drug development, the asymmetric reaction screening, and the structure determination of natural compounds. Although various methods, such as X-ray crystallography and NMR spectroscopy, are used to determine the AC, a simple and cheap alternative method is always anticipated. So far, electronic circular dichroism (ECD) spectroscopy has been widely used to ascertain the AC and enantiomeric excess (ee) values by applying appropriate organic probes. Here, circularly polarized luminescence (CPL) spectroscopy was applied to determine the AC and ee values of a series of amino acid and amino alcohol. The measurements were conducted by mixing the amino acids or amino alcohols with an achiral 1-hydroxy-2-naphthaldehyde. Upon in situ formation of the Schiff base complexes, the system showed emission enhancement and CPL in the presence of Al3+ , whose intensity and sign can be used to assign the chiral sense of the amino acids and amino alcohols. The authenticity of the method was further compared with the established CD spectroscopy, revealing that CPL spectra of formed Al3+ complex were effective to determine the AC of chiral species.

2.
Medicine (Baltimore) ; 98(50): e18213, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31852078

RESUMO

We aimed to investigate the association between excess body mass index (BMI) and papillary thyroid cancer (PTC) in an operative population, and the impact of higher BMI on clinicopathological aggressiveness of PTC.Charts of 10,844 consecutive patients with thyroid nodules undergoing partial or total thyroidectomy between 1993 and 2015 were reviewed. Patients diagnosed with PTC were stratified in 4 groups: BMI < 18.5 (underweight), 18.5 ≤ BMI < 24 (normal-weight), 24 ≤ BMI < 28 (overweight) and BMI ≥ 28(obese). The impacts of high BMI on prevalence and clinicopathological parameters of PTC were retrospectively analyzed in both univariate and multivariate binary logistic regression analysis.For every 5-unit increase in body mass, the odds of risk-adjusted malignance increased by 36.6%. The individuals who were obese and overweight were associated with high risk of thyroid cancer [odds ratio (OR)= 1.982, P < .001; OR= 1.377, P < .001; respectively] compared to normal weight patients, and this positive association was found in both genders. Obesity was independent predictors for tumors larger than 1 cm (OR = 1.562, P < .001) and multifocality (OR = 1.616, P < .001). However, there was no difference in cervical lymph node (LN) metastasis among BMI groups. Crude analysis showed BMI was associated with advanced tumor-node-metastasis (TNM) stage (relative risk, approximately 1.23 per 5 BMI units, P < .001), but this association disappeared after adjusting for confounding factors.Obesity was significantly associated with the risk of PTC in a large, operative population. Higher BMI was significantly associated with larger tumor size and multifocal tumor.


Assuntos
Índice de Massa Corporal , Obesidade/complicações , Câncer Papilífero da Tireoide/diagnóstico , Neoplasias da Glândula Tireoide/diagnóstico , Tireoidectomia/métodos , Estudos de Casos e Controles , China/epidemiologia , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Obesidade/epidemiologia , Estudos Retrospectivos , Câncer Papilífero da Tireoide/etiologia , Câncer Papilífero da Tireoide/cirurgia , Neoplasias da Glândula Tireoide/etiologia , Neoplasias da Glândula Tireoide/cirurgia
3.
BMC Biol ; 17(1): 73, 2019 09 14.
Artigo em Inglês | MEDLINE | ID: mdl-31521166

RESUMO

BACKGROUND: Spindle microtubule organization, regulated by microtubule-associated proteins, is critical for cell division. Proper organization of kinetochore fiber (K-fiber), connecting spindle poles and kinetochores, is a prerequisite for precise chromosomal alignment and faithful genetic material transmission. However, the mechanisms of K-fiber organization and dynamic maintenance are still not fully understood. RESULTS: We reveal that two previously uncharacterized coiled-coil domain proteins CCDC74A and CCDC74B (CCDC74A/B) are spindle-localized proteins in mammalian cells. They bind directly to microtubules through two separate domains and bundle microtubules both in vivo and in vitro. These functions are required for K-fiber organization, bipolar spindle formation, and chromosomal alignment. Moreover, CCDC74A/B form homodimers in vivo, and their self-association activity is necessary for microtubule bundling and K-fiber formation. CONCLUSIONS: We characterize CCDC74A and CCDC74B as microtubule-associated proteins that localize to spindles and are important K-fiber crosslinkers required for bipolar spindle formation and chromosome alignment.

4.
J Surg Case Rep ; 2019(8): rjz247, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31528329

RESUMO

Ciliated muconodular papillary tumor (CMPT) is a peripheral non-endobronchial lung nodule, consisting of ciliated columnar cells and goblet cells with basaloid cell proliferation. Only about 50 cases confirmed by surgery have been reported in English literature worldwide. We present two surgical cases of CMPT in this report. Two patients presented with abnormal computed tomography findings but no obvious symptoms. The first patient's intraoperative frozen examination was unable to distinguish benignity from malignancy, and he received lobectomy. The other patient's intraoperative frozen examination indicated adenocarcinoma, but she received wedge resection for her refusal to lobectomy. The two patients' postoperative pathological analysis finally confirmed the diagnosis of CMPT. We believe that our cases may be essential for pathologists and surgeons to improve their understanding.

5.
BMJ Open ; 9(1): e023334, 2019 01 25.
Artigo em Inglês | MEDLINE | ID: mdl-30782703

RESUMO

OBJECTIVES: This study aims to summarise the features and trends of thyroid carcinoma in the past two decades in China. DESIGN, SETTING AND PARTICIPANTS: Clinical data obtained from 10 798 patients treated by thyroidectomy from 1994 to 2015 at the Department of General Surgery of the People's Liberation Army General Hospital, Beijing, China were retrospectively analysed. OUTCOME MEASURES: Incidence and histopathological features of thyroid cancer were compared and the risk factors for local lymph node metastasis analysed. RESULTS: Our data indicated a significant increase in the detection of thyroid cancer (from 16.8% to 69.8%, p<0.01). Among the 5235 thyroid cancer cases, papillary thyroid carcinoma (PTC) was the most common histotype, accounting for 95.1% of all malignancies over the 22-year period. Among the 4979 PTCs, micro-PTCs (mPTC) with the largest diameter ≤10 mm has gradually become the dominant form, and its percentage in PTCs has increased from 13.3% in the biennial period of 1994-1995 to 51.2% in 2010-2011. Furthermore, the size of the tumour has decreased significantly from 2.3±1.1 cm in 1994 to 1.2±0.9 cm in the largest diameter (p<0.01), while the average age at diagnosis and female dominance remained unchanged during the period. Logistic regression showed that tumour nodules>1 cm and male gender were the main risk factors for local lymph node metastasis (LNM), whereas patients over 45 years had lower risk. CONCLUSIONS: During the 22-year period, an increased detection of thyroid cancer, particularly mPTC, was found while the occurrence of LNM decreased. Our results suggest that the current preoperative diagnosis and risk stratification are adequate, supporting the published guidelines for the diagnosis of thyroid cancer.

6.
Diagn Cytopathol ; 47(3): 187-193, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30499203

RESUMO

BACKGROUND: Thyroid nodules are very common. Ultrasound (US) and fine needle aspiration (FNA) are both integral in cancer screening. This study investigated the concordance between thyroid nodule sizes measured by US and gross pathologic examination and their relationship with malignancy. METHODS: A retrospective design was used to select consecutive patients with proven carcinoma of the thyroid. The number and maximum diameter of nodules, rates and types of malignancy, as well as predictors of malignancy were determined. RESULTS: The 10 944 patients examined had 15 283 thyroid nodules, 44.6% of which were malignant. Of the 4449 nodules sampled by FNA and the 8748 not sampled by FNA, 76.5% and 30.5% were malignant, respectively. The sensitivity, specificity, positive and negative predictive values (NPVs), and overall accuracy of FNA based on final pathology were 97.9%, 96.3%, 98.8%, 93.5%, and 97.5%, respectively. Nodule sizes determined by US were comparable with most nodules having either the same size range (n = 2959, 77.7%) or differing only by one size range (n = 770, 20.5%). CONCLUSIONS: Thyroid nodule size is inversely related to malignancy risk, as larger nodules have lower malignancy rates. Nodule size estimated by US shows relatively good correlation with final pathologic size. However, thyroid nodules should undergo FNA regardless of size. If the FNA is not benign, nodule size should influence therapeutic decision making.


Assuntos
Nódulo da Glândula Tireoide/diagnóstico por imagem , Nódulo da Glândula Tireoide/patologia , Adulto , Idoso , Biópsia por Agulha Fina , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Sensibilidade e Especificidade , Ultrassonografia
7.
Sci Rep ; 7(1): 15832, 2017 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-29158505

RESUMO

The study aimed to investigate the gender-related differences of disease onset, age distribution, blood type, clinical characteristics, and malignant behaviors of differentiated thyroid carcinoma (DTC) in Chinese patients. A total of 7385 consecutive thyroid cancer patients who underwent thyroidectomy were retrospectively reviewed. 4087 (55.3%) were diagnosed as benign and the other (3298, 44.7%) were as malignant. DTC accounted for 97.6% in the malignant tumor. More single nodules turned out to be DTC in male compared to multiple nodules (46.9% vs. 40.4%, P = 0.004). The proportion increased along with the increase of year during 2000-2013, which was from 7.5% to 68.1% in males and from 16.2% to 66.7% in females. The level of preoperative TSH was significantly higher in patients with DTC compared to the patients with benign (1.97 vs. 1.57 mIU/L, P < 0.001). The proportion of thyroid cancer was dominated in blood type B and the lowest incidence in blood type A in male, the difference was not statistically significant. The results showed that age, nodule number, BMI and serum TSH were the related factors for DTC. More aggressive behaviors of DTC were observed in male patients, and more attention should be focused on the timely diagnosis and treatment of these patients.


Assuntos
Adenocarcinoma/epidemiologia , Carcinoma/epidemiologia , Neoplasias da Glândula Tireoide/epidemiologia , Adenocarcinoma/sangue , Adenocarcinoma/patologia , Adenocarcinoma/cirurgia , Adulto , Fatores Etários , Idoso , Tipagem e Reações Cruzadas Sanguíneas , Carcinoma/sangue , Carcinoma/patologia , Carcinoma/cirurgia , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Caracteres Sexuais , Neoplasias da Glândula Tireoide/sangue , Neoplasias da Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/cirurgia , Nódulo da Glândula Tireoide/patologia , Nódulo da Glândula Tireoide/cirurgia , Tireoidectomia , Tireotropina/sangue , Adulto Jovem
8.
Plant Sci ; 263: 177-182, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28818373

RESUMO

In plants, the enzymes fatty acid dehydrogenase 2 (FAD2) and fatty acid elongase 1 (FAE1) have been shown in previous studies to play important roles in the de novo biosynthesis of fatty acids. However, the effects of depressed expression of FAD2 and FAE1 on seed storage compounds accumulation remains to be elucidated. In this study, we produced RNA interfering transgenic rapeseeds lines, BnFAD2-Ri, BnFAE1-Ri and BnFAD2/BnFAE1-Ri, which exhibited depressed expression of the BnFAD2 and BnFAE1 genes under the control of seed-specific napin A promoter. These transgenic rapeseeds showed normal growth and development as compared with the wild type (CY2). Depressed expression of BnFAD2 and BnFAE1 genes modified fatty acid profiles, leading to increased oleic acid and decreased erucic acid contents in transgenic seeds. Consistent with these results, the ratios of C18:1/C18:2 and C18:1/C18:3 in C18 unsaturated fatty acids were greatly increased due to increased oleic acid content in transgenic seeds. Moreover, depressed expression of BnFAD2 and BnFAE1 genes resulted in slightly decreased oil contents and increased protein contents in transgenic seeds. Our results demonstrated that depressed expression of BnFAD2 and BnFAE1 greatly improves seed nutritional quality by modulating the fatty acid metabolism and storage products accumulation and that BnFAD2 and BnFAE1 are reliable targets for genetic improvement of rapeseed in seed nutritional quality.


Assuntos
Brassica napus/genética , Ácidos Graxos/metabolismo , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/metabolismo , Brassica napus/fisiologia , Brassica rapa/genética , Brassica rapa/fisiologia , Ácidos Erúcicos/metabolismo , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas , Regiões Promotoras Genéticas/genética , Interferência de RNA , Sementes/genética , Sementes/fisiologia
9.
G3 (Bethesda) ; 6(4): 1073-81, 2016 04 07.
Artigo em Inglês | MEDLINE | ID: mdl-26896439

RESUMO

Seed coat color is determined by the type of pigment deposited in the seed coat cells. It is related to important agronomic traits of seeds such as seed dormancy, longevity, oil content, protein content and fiber content. In Brassica napus, inheritance of seed coat color is related to maternal effects and pollen effects (xenia effects). In this research we isolated a mutation of yellow seeded B. napus controlled by a single Mendelian locus, which is named Embryonal Control of Yellow seed coat 1 (Ecy1). Microscopy of transverse sections of the mature seed show that pigment is deposited only in the outer layer of the seed coat. Using Illumina Hisequation 2000 sequencing technology, a total of 12 GB clean data, 116× coverage of coding sequences of B. napus, was achieved from seeds 26 d after pollination (DAP). It was assembled into 172,238 independent transcripts, and 55,637 unigenes. A total of 139 orthologous genes of Arabidopsis transparent testa (TT) genes were mapped in silico to 19 chromosomes of B. napus Only 49 of the TT orthologous genes are transcribed in seeds. However transcription of all orthologs was independent of embryonal control of seed coat color. Only 55 genes were found to be differentially expressed between brown seeds and the yellow mutant. Of these 55, 50 were upregulated and five were downregulated in yellow seeds as compared to their brown counterparts. By KEGG classification, 14 metabolic pathways were significantly enriched. Of these, five pathways: phenylpropanoid biosynthesis, cyanoamino acid metabolism, plant hormone signal transduction, metabolic pathways, and biosynthesis of secondary metabolites, were related with seed coat pigmentation. Free amino acid quantification showed that Ala and Phe were present at higher levels in the embryos of yellow seeds as compared to those of brown seeds. This increase was not observed in the seed coat. Moreover, the excess amount of free Ala was exactly twice that of Phe in the embryo. The pigment substrate chalcone is synthesized from two molecules of Ala and one molecule of Phe. The correlation between accumulation of Ala and Phe, and disappearance of pigment in the yellow seeded mutant, suggests that embryonal control of seed coat color is related with Phe and Ala metabolism in the embryo of B. napus.


Assuntos
Alanina/metabolismo , Brassica napus/genética , Brassica napus/metabolismo , Fenilalanina/metabolismo , Locos de Características Quantitativas , Sementes/genética , Sementes/metabolismo , Arabidopsis/genética , Mapeamento Cromossômico , Biologia Computacional/métodos , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Estudos de Associação Genética , Genótipo , Sequenciamento de Nucleotídeos em Larga Escala , Mutação , Pigmentação/genética , Reprodutibilidade dos Testes , Sementes/ultraestrutura , Transcriptoma
10.
Oncotarget ; 6(37): 40081-94, 2015 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-26472025

RESUMO

High expression of immunoglobulin G (IgG) in many non-B cell malignancies and its non-conventional roles in promoting proliferation and survival of cancer cells have been demonstrated. However, the precise function of non-B IgG remains incompletely understood. Here we define the antigen specificity of RP215, a monoclonal antibody that specifically recognizes the IgG in cancer cells. Using RP215, our study shows that IgG is overexpressed in cancer cells of epithelial lineage, especially cells with cancer stem/progenitor cell-like features. The RP215-recognized IgG is primarily localized on the cell surface, particularly lamellipodia-like structures. Cells with high IgG display higher migration, increased invasiveness and metastasis, and enhanced self-renewal and tumorgenecity ability in vitro and in vivo. Importantly, depletion of IgG in breast cancer leads to reduced adhesion, invasion and self-renewal and increased apoptosis of cancer cells. We conclude that high expression of IgG is a novel biomarker of tumor progression, metastasis and cancer stem cell maintenance and demonstrate the potential therapeutic benefits of RP215-recognized IgG targeted strategy.


Assuntos
Neoplasias da Mama/imunologia , Células Epiteliais/imunologia , Imunoglobulina G/imunologia , Células-Tronco Neoplásicas/imunologia , Animais , Anticorpos Monoclonais/imunologia , Western Blotting , Neoplasias da Mama/genética , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Movimento Celular/genética , Movimento Celular/imunologia , Proliferação de Células/genética , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Feminino , Expressão Gênica/imunologia , Humanos , Imunoglobulina G/biossíntese , Imunoglobulina G/genética , Imuno-Histoquímica , Células MCF-7 , Metaloproteinase 2 da Matriz/genética , Metaloproteinase 2 da Matriz/imunologia , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/genética , Metaloproteinase 9 da Matriz/imunologia , Metaloproteinase 9 da Matriz/metabolismo , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos NOD , Camundongos Nus , Camundongos SCID , Metástase Neoplásica , Células-Tronco Neoplásicas/metabolismo , Células-Tronco Neoplásicas/patologia , Interferência de RNA , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transplante Heterólogo
11.
Medicine (Baltimore) ; 94(21): e841, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-26020388

RESUMO

The diagnosis of malignant thyroid nodules is still a clinical challenge. This study aimed to determine the ultrasonographic characteristics of papillary thyroid carcinoma. The ultrasonographic and pathological data of 2453 thyroid nodules in a cohort of 1895 Chinese patients who underwent thyroidectomy from January 2010 to December 2012 were retrospectively reviewed. Anteroposterior and transversal (AP/TR) diameters ≥1, solid structure, infiltrative margins, hypoechoic appearance, and microcalcifications were more common in malignant nodules than in benign nodules (P < 0.01). These ultrasonographic features were independent risk factors of malignancy (P < 0.01) as determined by logistic regression analysis. Based on multivariate analysis, these characteristics were also present in large nodules (diameter >10 mm). However, in small nodules (diameter ≤10 mm), only AP/TR ≥1 and infiltrative margins were independent risk factors of malignancy (P < 0.01). Ultrasonography is of high diagnostic value for malignant thyroid nodules and may help to improve the differential diagnosis. Small and large nodules have distinct ultrasonographic features.


Assuntos
Carcinoma Papilar/diagnóstico por imagem , Carcinoma Papilar/diagnóstico , Carcinoma/diagnóstico por imagem , Carcinoma/diagnóstico , Neoplasias da Glândula Tireoide/diagnóstico por imagem , Neoplasias da Glândula Tireoide/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biópsia por Agulha Fina , Calcinose , Carcinoma/cirurgia , Carcinoma Papilar/cirurgia , Criança , China , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Câncer Papilífero da Tireoide , Neoplasias da Glândula Tireoide/cirurgia , Ultrassonografia , Adulto Jovem
12.
Oncol Lett ; 4(2): 245-246, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22844362

RESUMO

The occurrence of a solitary plasmacytoma in the conjunctiva is unusual. This relatively uncommon neoplastic disorder occurs more frequently in other organs and is derived from B-lymphocytes. In the present study the case of a 17-year-old female, who had a lesion of the lower fornix conjunctiva in the left eye, is presented. The lesion was resected and the patient underwent amniotic membrane transplantation. Pathological examination of the specimen showed a plasmacytoma. Bone marrow aspirate showed that the cellularity and number of plasma cells were normal, and no myeloma cells were observed. A complete skeletal survey failed to show bony masses or osteolytic events. Following surgery, the patient was provided with a course of radiotherapy. During the follow-up period of 12 months the lesion did not recur.

13.
Eur J Radiol ; 81(6): 1231-7, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21420817

RESUMO

BACKGROUND: Rosai-Dorfman disease (RDD) in sellar/suprasellar region is a rare intracranial disorder. The diagnostic evaluation of this condition using magnetic resonance imaging (MRI) has seldom been described previously. The purpose of our study was to describe MRI characteristics of sellar/suprasellar region RDD. METHODS: Five patients with proved sellar/suprasellar region RDD from May 2005 to March 2010 were retrospectively reviewed. All the patients had undergone magnetic resonance scanning. The number, location, signal intensity (SI), and enhancement pattern of the lesions on MRI were retrospectively evaluated. RESULTS: Pathological diagnosis of RDD was achieved in all 5 cases including 4 by surgery and 1 by biopsy. The most common presenting symptoms were headache (n = 4) and blurred vision (n = 3). On MRI, isolated suprasellar lesion was found in 2 cases. Suprasellar lesion combined with intrasellar, dural, intra-axial and orbital lesions was found in 3 cases. All lesions showed homogeneous isointense SI on T1-weighted images and hypointense to isointense SI on T2-weighted images except one lesion in the midbrain with slight hyperintense SI on T2-weighted images. All lesions showed homogeneous enhancement. CONCLUSION: Homogenously enhancing sellar/suprasellar masses of hypointense to isointense SI on T2-weighted images are suggestive of RDD, and central hypointensity on T2-weighted images may be a specific finding. Intra-axial and extra-axial involvements may coexist with sellar/suprasellar region RDD. Although radiological findings can provide some evidence for this rare entity, differential diagnosis is still needed.


Assuntos
Histiocitose Sinusal/patologia , Imagem por Ressonância Magnética/métodos , Sela Túrcica/patologia , Adolescente , Adulto , Idoso , Biópsia , Pré-Escolar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
14.
Neuropathology ; 31(4): 414-20, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21062362

RESUMO

Meningeal melanocytoma is an uncommon pigmented neoplasm that affects the CNS and develops in the cranial and spinal leptomeninges. Here we report on a case of malignant transformation of intracranial supratentorial meningeal melanocytoma which recurred after 3 years as malignant melanoma. This case demonstrates that the biological behavior of melanocytoma is uncertain and that these lesions may recur as malignant melanoma.


Assuntos
Transformação Celular Neoplásica/patologia , Melanócitos/patologia , Melanoma/patologia , Neoplasias Meníngeas/patologia , Lobo Temporal/patologia , Craniotomia , Humanos , Masculino , Melanoma/cirurgia , Neoplasias Meníngeas/cirurgia , Lobo Temporal/cirurgia , Resultado do Tratamento
15.
J Neurooncol ; 104(1): 387-94, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21190059

RESUMO

Primary spinal cord oligodendroglial tumor is very rare, and in only one patient with spinal cord oligodendroglioma has a deletion of chromosome 1p/19q been reported. We present the case of an 18-year-old girl, who had one-year lower back pain and one-month lower limb weakness. Magnetic resonance images of the spinal cord showed an intramedullary mass from level T8 to T10, which was then radically removed. Histology revealed an anaplastic oligodendroglioma. The patient was treated with radiotherapy postoperatively. Eight months after the treatment, follow-up magnetic resonance images disclosed an enhancing intramedullary mass at level T4-T8; recurrence of the tumor was therefore diagnosed. Maximum surgical removal of the recurrent tumor was performed, diagnosis of anaplastic oligodendroglioma was made, and a chromosome 1p deletion was determined by FISH. After treatment with temozolomide for six months, the patient had a remarkable improvement of her lower limb symptoms, and complete imaging regression of the residual tumor showed no evidence of recurrence at any other sites. The most recent MRI of brain and spinal cord showed postoperative changes without evidence of tumor recurrence of the spine and oligodendrogliomatosis along the cerebral-spinal axis. To our knowledge, this is the first report of a recurrent anaplastic oligodendroglioma with 1p deletion occurring in the spinal cord. It is also the first case of the patient with recurrent intramedullary anaplastic oligodendroglioma who had a significant clinical improvement and complete imaging remission after subtotal resection then treatment with temozolomide chemotherapy.


Assuntos
Antineoplásicos Alquilantes/uso terapêutico , Deleção Cromossômica , Cromossomos Humanos Par 1 , Dacarbazina/análogos & derivados , Oligodendroglioma/tratamento farmacológico , Neoplasias da Medula Espinal/tratamento farmacológico , Adolescente , Dacarbazina/uso terapêutico , Feminino , Humanos , Oligodendroglioma/genética , Neoplasias da Medula Espinal/genética , Temozolomida
16.
Acta Neurochir (Wien) ; 153(4): 859-67, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21161294

RESUMO

Rosai-Dorfman disease [RDD] is a condition of an unknown etiology, characterized by proliferation of histiocytes. Not uncommonly extranodal lesions occur, but sellar involvement is very rare. Treatment of this lesion is complicated, especially in cases of recurrences at multiple intracranial sites. We report two cases of RDD in the sellar region with intracranial recurrences to illustrate the clinicopathological features and considerations of the optimal management.


Assuntos
Encefalopatias/diagnóstico , Encefalopatias/cirurgia , Endoscopia , Histiocitose Sinusal/diagnóstico , Histiocitose Sinusal/cirurgia , Microcirurgia , Doenças da Hipófise/diagnóstico , Doenças da Hipófise/cirurgia , Sela Túrcica , Adulto , Criança , Feminino , Seguimentos , Humanos , Hipopituitarismo/diagnóstico , Hipopituitarismo/cirurgia , Processamento de Imagem Assistida por Computador , Imagem por Ressonância Magnética , Masculino , Recidiva , Reoperação , Tomografia Computadorizada por Raios X
17.
Zhonghua Xin Xue Guan Bing Za Zhi ; 39(11): 1021-3, 2011 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-22336455

RESUMO

OBJECTIVE: To analyze the clinic-pathologic features of elderly myocardial infarction patients (> 60 years) with and without left ventricular aneurysm formation. METHODS: Between January 1980 and October 2009, 107 myocardial infarction patients were divided into aneurysm group (n = 31) and non-aneurysm group (n = 76) according to autopsy results and the clinic-pathologic features of the two groups were compared. RESULTS: Previous angina pectoris history was significantly less in aneurysm group than in non-aneurysm group [45.2% (14/31) vs. 92.1% (70/76), P = 0.047]. Incidence of hypertension was significantly higher in aneurysm group than in non-aneurysm group [77.4% (24/31) vs. 36.8% (28/76), P = 0.033]. The percentage of single-vessel disease [54.8% (17/31) vs. 23.7% (18/76), P = 0.033] and the LAD disease [96.8% (30/31) vs. 51.3% (39/76), P = 0.048] were both significantly higher in aneurysm group than in non-aneurysm group. Heart failure and ventricular arrhythmias were more likely the cause of death in patients with aneurysm than patients without aneurysm [56.3% (18/31) vs. 19.7% (15/76), P = 0.007]. Aneurysm mostly located in left ventricular anterior wall and apex. CONCLUSIONS: Our results suggest that patients with left ventricular aneurysm formation are more likely to have hypertension, single-vessel disease and LAD disease, heart failure and ventricular arrhythmias but less previous angina pectoris than patients without left ventricular aneurysm formation. The common locations of ventricular aneurysm formation were left ventricular anterior wall and apex.


Assuntos
Aneurisma Cardíaco/patologia , Infarto do Miocárdio/patologia , Idoso , Idoso de 80 Anos ou mais , Feminino , Aneurisma Cardíaco/complicações , Ventrículos do Coração/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Infarto do Miocárdio/complicações
18.
Chin J Cancer Res ; 23(2): 118-22, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23483098

RESUMO

OBJECTIVE: To investigate the validity of CT perfusion in assessing angiogenic activity of lung cancer. METHODS: Fifty-six patients with lung cancer scheduled for elective surgical resection received 16-slice helical CT perfusion imaging. Time-density curve (TDC), blood flow (BF), blood volume (BV), mean transmit time (MTT) and permeability surface area product (PS) were calculated. 18F-deoxyglucose-positron emission tomography (FGD-PET) was carried out in 14 out of the 56 patients to calculate standardized uptake values (SUVs). Tumor microvessel density (MVD) was examined using CD34 immunohistochemical staining of the resected tumor tissue. Pearson's correlation analysis was used to evaluate potential correlation between CT perfusion parameters and MVD or SUV. RESULTS: Average time to peak height (TPH) of the TDCs (including two types of TDC) was 24.38±5.69 seconds. Average BF, BV, MTT and PS were 93.42±53.45 ml/100g/min,93.42±53.45 ml/100g,6.83±4.51 s and 31.92±18.73 ml/100g/min, respectively. Average MVD was 62.04±29.06/HPF. The mean SUV was 6.33±3.26. BF was positively correlated with MVD (r=0.620,P<0.01) and SUV (r=0.891, P<0.01). PS was also positively correlated with SUV (r=0.720, P<0.05). A positive correlation was also observed between tumor MVD and SUV (r=0.915, P<0.01). CONCLUSIONS: CT perfusion imaging is a reliable tool to evaluate the tumor neovascularity of lung cancer.

19.
Chemosphere ; 75(2): 187-92, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19157497

RESUMO

Ozone have been widely used as pre-oxidant before conventional water treatment processes, while some controversial results on the effects of preozonation on coagulation and particle stabilization, have been described. The performance of coagulated flocs can be substantially influenced by preozonation which can be ascribed to the variation of particles or organic substances in water. In order to elucidate the effects of preozonation as an aid in subsequent coagulation, the synthetic water composed of kaolin suspension and humic acid was coagulated after preozonation. The properties of floc, such as, size, fractal dimension and effective density, were investigated using laser light scattering analyzer and settling column measurement. It was found that the suspended particles had not been destabilized by preozonation as regarding the slight variation of zeta potential. As the ozone dose was less than 0.53mgO(3)mg(-1) C, floc size was not influenced apparently, while, as the ozone dose increased, the flocs were broken and the size decreased remarkably. Specifically, the fractal dimensions of flocs increased with increasing ozone dose, resulting in more densely packed flocs with low branch structure. Additionally, in terms of settling characteristics, the optimal pre-ozone dose was found to be 0.53mgO(3)mg(-1) C, which led to highest effective density of flocs with best liquid-solid separation efficiency.


Assuntos
Floculação , Ozônio/química , Purificação da Água/métodos , Monitoramento Ambiental
20.
J Colloid Interface Sci ; 328(2): 464-9, 2008 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-18848335

RESUMO

A method based on a simple linear regression fitting was proposed and used to determine the type, the chronological sequence, and the relative importance of individual fouling mechanisms in experiments on the dead-end filtration of colloidal suspensions with membranes ranging from loose ultrafiltration (UF) to nanofiltration (NF) to non-porous reverse osmosis (RO). For all membranes, flux decline was consistent with one or more pore blocking mechanisms during the earlier stages and with the cake filtration mechanism during the later stages of filtration. For ultrafiltration membranes, pore blocking was identified as the largest contributor to the observed flux decline. The chronological sequence of blocking mechanisms was interpreted to depend on the size distribution and surface density of membrane pores. For salt-rejecting membranes, the flux decline during the earlier stages of filtration was attributed to either intermediate blocking of relatively more permeable areas of the membrane skin, or to the cake filtration in its early transient stages, or a combination of these two mechanisms. The findings emphasize the practical importance of the clear identification of, and differentiation between mechanisms of pore blocking and cake formation as determining the potential for the irreversible fouling of membranes and the efficiency of membrane cleaning.

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