Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 99
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Integr Cancer Ther ; 18: 1534735419880275, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31595795

RESUMO

Background: Ganoderma lucidum has been used in Chinese medicine for thousands years to improve health and to promote longevity. One important function of G lucidum is to modulate the immune system. However, the underlying mechanism is not well understood. Programmed cell death protein 1 (PD-1) is a cell surface protein present in certain immune cells (eg, B- and Tcells) and plays an important role in modulating the immune response. The role of PD-1 protein in G lucidum-mediated immunomodulation is unknown. Methods: Cultured human Blymphocytes and extract prepared from G lucidum spores (GLE) were used to determine PD-1 protein in G lucidum-mediated immunomodulation. Both western blotting and immunofluorescence (IF) microscopy assays were used to determine the effect of GLE treatment on PD-1 protein expression. A reverse transcription-based quantitative polymerase chain reaction (real-time PCR) assay was used to determine the effect of GLE on transcription of pdcd-1 gene. Results: Both our western blotting and IF staining results demonstrated great reduction in PD-1 protein and in proportion of PD-1+ cells in these B-lymphocytes. Our real-time PCR results indicated that this PD-1 protein reduction was not caused by a transcriptional inhibition of the gene. In addition, our western blotting study further revealed that the GLE treatment caused an increase in expression of CCL5 chemokine in the cultured B-lymphocytes. Conclusions: PD-1 protein is an important target of G lucidum-mediated immunomodulation. G lucidum and its bioactive compounds can be developed into novel immunomodulators for prevention and treatment of cancer and many other diseases.

2.
Angew Chem Int Ed Engl ; 58(41): 14534-14538, 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31373130

RESUMO

Adsorption plays a critical role in surface and interface processes. Fractional surface coverage and adsorption free energy are two essential parameters of molecular adsorption. However, although adsorption at the solid-gas interface has been well-studied, and some adsorption models were proposed more than a century ago, challenges remain for the experimental investigation of molecular adsorption at the solid-liquid interface. Herein, we report the statistical and quantitative single-molecule measurement of adsorption at the solid-liquid interface by using the single-molecule break junction technique. The fractional surface coverage was extracted from the analysis of junction formation probability so that the adsorption free energy could be calculated by referring to the Langmuir isotherm. In the case of three prototypical molecules with terminal methylthio, pyridyl, and amino groups, the adsorption free energies were found to be 32.5, 33.9, and 28.3 kJ mol-1 , respectively, which are consistent with DFT calculations.

3.
Nano Lett ; 19(9): 6144-6151, 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31438678

RESUMO

The heterostructures of the ferromagnet (Cr2Te3) and topological insulator (Bi2Te3) have been grown by molecular beam epitaxy. The topological Hall effect as evidence of the existence of magnetic skyrmions has been observed in the samples in which Cr2Te3 was grown on top of Bi2Te3. Detailed structural characterizations have unambiguously revealed the presence of intercalated Bi bilayer nanosheets right at the interface of those samples. The atomistic spin-dynamics simulations have further confirmed the existence of magnetic skyrmions in such systems. The heterostructures of ferromagnet and topological insulator that host magnetic skyrmions may provide an important building block for next generation of spintronics devices.

4.
Chin J Nat Med ; 17(6): 413-423, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31262454

RESUMO

Isoflavones are widely consumed by people around the world in the form of soy products, dietary supplements and drugs. Many isoflavones or related crude extracts have been reported to exert pain-relief activities, but the mechanism remains unclear. Voltage-gated sodium channels (VGSCs) play important roles in excitability of pain sensing neurons and many of them are important nociceptors. Here, we report that several isoflavones including 3'-methoxydaidzein (3MOD), genistein (GEN) and daidzein (DAI) show abilities to block VGSCs and thus to attenuate chemicals and heat induced acute pain or chronic constriction injury (CCI) induced pain hypersensitivity in mice. Especially, 3MOD shows strong analgesic potential without inducing addiction through inhibiting subtypes NaV1.7, NaV1.8 and NaV1.3 with the IC50 of 181 ± 14, 397 ± 26, and 505 ± 46 nmol·L-1, respectively, providing a promising compound or parent structure for the treatment of pain pathologies. This study reveals a pain-alleviating mechanism of dietary isoflavones and may provide a convenient avenue to alleviate pain.

5.
J Alzheimers Dis ; 68(4): 1687-1697, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30958378

RESUMO

Traumatic brain injury (TBI), a brain disorder that causes death and long-term disability in humans, is increasing in prevalence, though there is a lack of protective or therapeutic strategies for mitigating the damage after TBI and for preserving neurological functionality. Microglia cells play a key role in neuroinflammation following TBI, but their regulation and polarization by a member of the vascular endothelial growth factor (VEGF) family, VEGF-C, is unknown. Here, we show that VEGF-C induced M2 polarization in a murine microglia cell line, BV-2, in vitro, by a mechanism that required signaling from its unique receptor, VEGF receptor 3 (VEGFR3). Moreover, in a TBI model in rats, VEGF-C administration induced M2 polarization of microglia cells, significantly improved motor deficits after experimental TBI, and significantly improved neurological function following TBI, likely through a reduction in cell apoptosis. Together, our data reveal a previously unknown role of VEGF-C/VEGFR3 signaling in the regulation of post-TBI microglia cell polarization, which appears to be crucial for recovery from TBI.

6.
Artigo em Inglês | MEDLINE | ID: mdl-30398850

RESUMO

Development of highly efficient catalysts based on transition metal oxides (TMOs) is desirable and remains a big challenge for lithium-oxygen (Li-O2) batteries. In the present work, atomic-thick TiO2(B) nanosheets decorated with ultrafine Co3O4 nanocrystals (Co3O4-TiO2(B)) were synthesized and utilized as cathode catalyst in Li-O2 batteries by designing a hybrid and inducing oxygen vacancies. The XPS characterization results suggested that the introduction of Co3O4 nanocrystals could induce numerous oxygen vacancies in the TiO2(B) nanosheets through Co doping in the hybrid catalyst. The subsequent electrochemical experiments indicated that the Li-O2 batteries with the prepared hybrid catalysts showed high specific capacity (11000 mAhg-1), and good cycling stability (200 cycles at a limited capacity of 1000 mAhg-1) with low polarization (above 2.7 V for discharge medium voltage and below 4.0 V for charge medium voltage within 80 cycles). Furthermore, a possible working mechanism was proposed for a better understanding of the high performance of Co3O4-TiO2(B) catalysts for the Li-O2 batteries. This work also provided new insights into designing efficient catalysts through interface engineering between 2D (two-dimensional) TMOs and 0D (zero-dimensional) TMOs for Li-O2 batteries or other catalysis-related fields.

8.
ACS Nano ; 12(4): 4010-4018, 2018 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-29658703

RESUMO

Two-dimensional (2D) layered transition-metal dichalcogenides (LTMDs) display various crystal phases with distinct symmetries, structures, and physical properties. Exploring and designing different structural phases in two dimensions could provide an avenue for switching material properties, aiming at practical applications for potential fields. Here we demonstrate a conceptually designed approach to narrow the band gap of MoSe2 and obtain a conductive red MoSe2 nanosheet. By introducing the high valence state of Mo species and constructing the Mo-O bonding on the surface of the MoSe2 nanosheets, the electronic properties can be modified and the conductivity is accordingly improved, an effect that significantly improves their lithium storage capacity and high-rate capability. We anticipate that the exploration of the conductive red MoSe2 with tunable band gap could help us unlock more potential crystal structures of LTMD-based and even other 2D materials for further applications.

9.
J Chin Med Assoc ; 81(7): 599-604, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29703517

RESUMO

BACKGROUND: Recent studies suggested that the gray-white matter ratio (GWR) determined from brain computed tomography (CT) scans may be a reliable predictor of poor neurological outcomes. The aim of study was to evaluate the association between the GWR and the outcomes in adult comatose cardiac arrest (CA) survivors in Chinese. METHODS: A total of 58 CA patients who had CT scans within 72 h of resuscitation between January 2011 and December 2015 were included in this single-center retrospective study. Gray and white matter attenuations (Hounsfield units) were measured, and the GWRs were calculated according to previous studies. The study analyzed the prognostic values of the GWRs in predicting poor outcomes (Cerebral Performance Category 3-5). RESULTS: The attenuation values of gray matter were significantly higher in the good outcome group than in the poor one. All GWRs were significantly higher in the good outcome group (p < 0.05). A GWR (basal ganglia) < 1.18 predicted poor outcomes with a sensitivity and specificity of 50.0% and 87.5%, respectively (p = 0.021). GWR (cerebrum) showed the best predictive performance when CT was performed within 24-72 h (p = 0.003). No significant differences were found between GWR and poor outcomes when CT was performed within the first 24 h. CONCLUSION: Low GWRs which were obtained from brain CT scans in comatose CA patients after restoration of spontaneous circulation were associated with poor neurological outcomes. GWR from brain CT can be a useful parameter for prognostic prediction aiding to an optimal clinical decision process in comatose CA survivors.

10.
Adv Mater ; 30(20): e1800515, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29603421

RESUMO

Both conductivity and mobility are essential to charge transfer by carrier transport layers (CTLs) in perovskite solar cells (PSCs). The defects derived from generally used ionic doping method lead to the degradation of carrier mobility and parasite recombinations. In this work, a novel molecular doping of NiOx hole transport layer (HTL) is realized successfully by 2,2'-(perfluoronaphthalene-2,6-diylidene)dimalononitrile (F6TCNNQ). Determined by X-ray photoelectron spectroscopy and ultraviolet photoelectron spectroscopy, the Fermi level (EF ) of NiOx HTLs is increased from -4.63 to -5.07 eV and valence band maximum (VBM)-EF declines from 0.58 to 0.29 eV after F6TCNNQ doping. The energy level offset between the VBMs of NiOx and perovskites declines from 0.18 to 0.04 eV. Combining with first-principle calculations, electrostatic force microscopy is applied for the first time to verify direct electron transfer from NiOx to F6TCNNQ. The average power conversion efficiency of CsFAMA mixed cation PSCs is boosted by ≈8% depending on F6TCNNQ-doped NiOx HTLs. Strikingly, the champion cell conversion efficiency of CsFAMA mixed cations and MAPbI3 -based devices gets to 20.86% and 19.75%, respectively. Different from passivation effect, the results offer an extremely promising molecular doping method for inorganic CTLs in PSCs. This methodology definitely paves a novel way to modulate the doping in hybrid electronics more than perovskite and organic solar cells.

11.
Front Pharmacol ; 9: 186, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29559913

RESUMO

Considering blood-sucking habits of leeches from surviving strategy of view, it can be hypothesized that leech saliva has analgesia or anesthesia functions for leeches to stay undetected by the host. However, no specific substance with analgesic function has been reported from leech saliva although clinical applications strongly indicated that leech therapy produces a strong and long lasting pain-reducing effect. Herein, a novel family of small peptides (HSTXs) including 11 members which show low similarity with known peptides was identified from salivary glands of the leech Haemadipsa sylvestris. A typical HSTX is composed of 22-25 amino acid residues including four half-cysteines, forming two intra-molecular disulfide bridges, and an amidated C-terminus. HSTX-I exerts significant analgesic function by specifically inhibiting voltage-gated sodium (NaV) channels (NaV1.8 and NaV1.9) which contribute to action potential electrogenesis in neurons and potential targets to develop analgesics. This study reveals that sodium channel inhibitors are analgesic substances in the leech. HSTXs are excellent candidates or templates for development of analgesics.

12.
J Cell Biochem ; 119(7): 5222-5232, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29240236

RESUMO

Mesoporous bioactive glass (MBG), a kind of porous materials with great osteoconductive and osteoinductive ability, shows promising application in bone tissue engineering due to its high specific surface area, orderly channel structure, and large pore volume. Here we reported that the proliferation, differentiation, and mineralization were promoted in MC3T3-E1 cells cultured on MBG which adsorbed with testosterone (MBG/T). We found that transcriptional activity of Runx2 which is a critical transcription factor is increased in MC3T3-E1 cells cultured on MBG/T. Intriguingly, we observed that ERK phosphorylation was enhanced in MC3T3-E1 cells cultured on MBG/T. We showed that activated Runx2 in MC3T3-E1 cells cultured on MBG/T is through Erk1/2 phosphorylation. Consistent with this result, we also found that the expression of osteoblastic marker genes were increased. Therefore, we concluded that osteoblast differentiation and mineralization was enhanced after cells cultured on MBG/T through Erk1/2-activated Runx2 pathway. Our findings provided that MBG/T is a potential material in the process of bone repair.

13.
Protein Sci ; 27(2): 431-440, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29076579

RESUMO

An efficient protein-folding pathway leading to target structure, and the avoidance of aggregation, is essential to protein evolution and de novo design; however, design details to achieve efficient folding and avoid aggregation are poorly understood. We report characterization of the thermally-induced aggregate of fibroblast growth factor-1 (FGF-1), a small globular protein, by solid-state NMR. NMR spectra are consistent with residual structure in the aggregate and provide evidence of a structured region that corresponds to the region of the folding nucleus. NMR data on aggregated FGF-1 also indicate the presence of unstructured regions that exhibit hydration-dependent dynamics and suggest that unstructured regions of aggregated FGF-1 lie outside the folding nucleus. Since it is known that regions outside the folding nucleus fold late in the folding pathway, we postulate that these regions unfold early in the unfolding pathway and that the partially folded state is more prone to intermolecular aggregation. This interpretation is further supported by comparison with a designed protein that shares the same FGF-1 folding nucleus sequence, but has different 1° structure outside the folding nucleus, and does not thermally aggregate. The results suggest that design of an efficient folding nucleus, and the avoidance of aggregation in the folding pathway, are potentially separable design criteria - the latter of which could principally focus upon the physicochemical properties of 1° structure outside the folding nucleus.

14.
J Org Chem ; 82(19): 10376-10387, 2017 10 06.
Artigo em Inglês | MEDLINE | ID: mdl-28877441

RESUMO

An efficient large-scale synthesis of acid 1, a penultimate precursor to the HCV NS5A inhibitor BMS-986097, along with the final API step are described. Three routes were devised for the synthesis of 1 at the various stages of the program. The third generation route, the one that proved scalable and is the main subject of this paper, features a one-step Michael addition of t-butyl 2-((diphenylmethylene)amino)acetate (24) to (E)-benzyl 4-(1-hydroxycyclopropyl)but-2-enoate (28) followed by cyclization and chiral separation to form 27c, the core skeleton of cap piece 1. The epimerization and chiral resolution of 27c followed by further synthetic manipulations involving the carbamate formation, lactone reduction and cyclization, afforded cyclopropyl pyran 1. A detailed study of diphenylmethane deprotection via acid hydrolysis as well as a key lactone to tetrahydropyran conversion, in order to avoid a side reaction that afforded an alternative cyclization product, are discussed. This synthesis was applied to the preparation of more than 100 g of the final API BMS-986097 for toxicology studies.


Assuntos
Antivirais/síntese química , Glicina/análogos & derivados , Imidazóis/síntese química , Piranos/farmacologia , Pirrolidinas/síntese química , Compostos de Espiro/síntese química , Proteínas não Estruturais Virais/antagonistas & inibidores , Antivirais/química , Antivirais/farmacologia , Imidazóis/química , Imidazóis/farmacologia , Estrutura Molecular , Piranos/síntese química , Piranos/química , Pirrolidinas/química , Pirrolidinas/farmacologia , Compostos de Espiro/química , Compostos de Espiro/farmacologia , Proteínas não Estruturais Virais/metabolismo
15.
Med Sci Monit ; 23: 4241-4251, 2017 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-28865235

RESUMO

BACKGROUND This study aimed to evaluate the effects of electro-acupuncture (EA) on neuroplasticity associated with the expressions of neurotrophic factors (NTFs) and their receptors in rats subjected to spinal cord transection (SCT). MATERIAL AND METHODS A total of 144 rats were randomly divided into 3 groups (n=48 per group): sham-operated group, SCT group, and EA (electro-acupuncture) group. Rats in SCT and EA groups received spinal cord transection at T10-T11 vertebral levels. Then, EA group rats received EA treatment. Reverse transcription polymerase chain reaction was used to detect NTFs and receptors at the mRNA level. In situ hybridization (ISH) and immunohistochemistry (IHC) were used to detect the expression of NTFs and their receptors. Basso, Beattie, Bresnahan (BBB) scores and cortical somato-sensory evoked potentials (CSEP) were evaluated to assess the recovery of motor and sensory functions. We also measured BDA (Biotinylated dextran amine) axonal tracing, CGRP (Calcitonin gene-related peptide), GAP-43 (Growth-associated protein), and synaptophysin immunohistochemistry (IHC). RESULTS EA treatment led to obvious improvement in hindlimb locomotor and sensory functions. CNTF, FGF-2, and TrkB mRNA were significantly upregulated, while NGF, PDGF, TGF-b1, IGF-1, TrkA, and TrkC mRNA were concomitantly downregulated in the caudal spinal segment (CSS) following EA. Immunohistochemistry demonstrated an increased number of CGRP fibers, GAP-43, and synaptophysin profiles in the CSS in the EA rats. CONCLUSIONS EA may promote the recovery of neuroplasticity in rats subjected to SCT. This could be attributed to the systematic regulation of NTFs and their receptors after EA.


Assuntos
Eletroacupuntura/métodos , Plasticidade Neuronal/efeitos dos fármacos , Traumatismos da Medula Espinal/terapia , Animais , Fatores de Crescimento Neural/análise , Fatores de Crescimento Neural/efeitos dos fármacos , Regeneração Nervosa/fisiologia , Plasticidade Neuronal/genética , Ratos , Ratos Sprague-Dawley , Recuperação de Função Fisiológica
16.
Nanotechnology ; 28(45): 455601, 2017 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-28891494

RESUMO

We have developed an incandescent Mo source to fabricate large-area single-crystalline MoSe2 thin films. The as-grown MoSe2 thin films were characterized using transmission electron microscopy, energy dispersive x-ray spectroscopy, atomic force microscopy, Raman spectroscopy, photoluminescence (PL), reflection high energy electron diffraction (RHEED) and angular resolved photoemission spectroscopy (ARPES). A new Raman characteristic peak at 1591 cm-1 was identified. Results from Raman spectroscopy, PL, RHEED and ARPES studies consistently reveal that large-area single crystalline mono-layer of MoSe2 could be achieved by this technique. This technique enjoys several advantages over conventional approaches and could be extended to the growth of other two-dimensional layered materials containing a low-vapor-pressure element.

17.
Phys Rev Lett ; 119(11): 116401, 2017 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-28949203

RESUMO

SnSe has emerged as an efficient thermoelectric material since a high value of the thermoelectric figure of merit (ZT) has been reported recently. Here we show with systematic angle resolved photoemission spectroscopy data that the low-lying electronic structures of undoped and hole-doped SnSe crystals exhibit noticeable temperature variation from 80 to 600 K. In particular, the hole effective masses for the two lowest lying valence band maxima are found to be very large and increase with decreasing temperature. Thermoelectric parameters derived from such hole-mass enhancement agree well with the transport values, indicating comprehensively a reduced impact of multivalley transport to the system's thermoelectric performance.

18.
Metab Brain Dis ; 32(5): 1427-1435, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28391551

RESUMO

Posttraumatic hydrocephalus (PTH) is a disorder of disturbed cerebrospinal fluid (CSF) dynamics after traumatic brain injury (TBI). It can lead to brain metabolic impairment and dysfunction and has a high risk of clinical deterioration and worse outcomes. The incidence and risk factors for the development of PTH after decompressive craniectomy (DC) has been assessed in previous studies, but rare studies identify patients with higher risk for PTH among all TBI patients. This study aimed to develop and validate a risk scoring system to predict PTH after TBI. Demographics, injury severity, duration of coma, radiologic findings, and DC were evaluated to determine the independent predictors of PTH during hospitalization until 6 months following TBI through logistic regression analysis. A risk stratification system was created by assigning a number of points for each predictor and validated in an independent cohort. The model accuracy was assessed by the area under the receiver operating characteristic curve (AUC). Of 526 patients in the derivation cohort, 57 (10.84%) developed PTH during 6 months follow up. Age > 50 yrs (Odd ratio [OR] = 1.91, 95% confidence interval [CI] 1.09-3.75, 4 points), duration of coma ≥1 w (OR = 5.68, 95% CI 2.57-13.47, 9 points), Fisher grade III (OR = 2.19, 95% CI 1.24-4.36, 5 points) or IV (OR = 3.87, 95% CI 1.93-8.43, 7 points), bilateral DC (OR = 6.13, 95% CI 2.82-18.14, 9 points), and extra herniation after DC (OR = 2.36, 95% CI 1.46-4.92, 5 points) were independently associated with PTH. Rates of PTH for the low- (0-12 points), intermediate- (13-22 points) and high-risk (23-34 points) groups were 1.16%, 35.19% and 78.57% (p < 0.0001). The corresponding rates in the validation cohort, where 17/175 (9.71%) developed PTH, were 1.35%, 37.50% and 81.82% (p < 0.0001). The risk score model exhibited good-excellent discrimination in both cohorts, with AUC of 0.839 versus 0.894 (derivation versus validation) and good calibration (Hosmer-Lemshow p = 0.56 versus 0.68). This model will be useful to identify patients at high risk for PTH who may be candidates for preventive interventions, and to improve their outcomes.


Assuntos
Hidrocefalia/epidemiologia , Hidrocefalia/etiologia , Adulto , Fatores Etários , Área Sob a Curva , China/epidemiologia , Estudos de Coortes , Craniectomia Descompressiva , Feminino , Escala de Coma de Glasgow , Hérnia/complicações , Hérnia/etiologia , Humanos , Hidrocefalia/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Modelos Estruturais , Valor Preditivo dos Testes , Prognóstico , Curva ROC , Reprodutibilidade dos Testes , Estudos Retrospectivos , Medição de Risco , Fatores de Risco , Tomografia Computadorizada por Raios X
19.
Biomed Mater ; 12(2): 025020, 2017 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-28452332

RESUMO

Mesoporous bioactive glass (MBG) has been demonstrated to play a vital role in bone tissue engineering due to its bioactivity, biocompatibility, and osteoinduction properties. Here, we report that MBG grafted with an amino group (MBG-NH2) and MBG-NH2 adsorbed epidermal growth factor (EGF) (MBG-NH2/EGF) sustained-release EGF, and MBG-NH2/EGF could accelerate osteoblast differentiation and mineralization in MC3T3-E1 cells. We found that MBG-NH2 could promote bone-like deposit formation and Ca deposition in vitro. Intriguingly, we observed that MBG-NH2/EGF enhanced MC3T3-E1 cell adhesion. We also showed that extracellular signal-regulated kinase 1/2 (ERK1/2) was phosphorylated when MC3T3-E1 cells were cultured on MBG-NH2/EGF. Interestingly, the transcription factor Runx2, important for osteoblast differentiation, was also activated when MC3T3-E1 cells were cultured on MBG-NH2/EGF. We showed that MC3T3-E1 cells cultured on MBG-NH2/EGF activating Runx2 was through ERK1/2 phosphorylation. Consistent with this survey, we observed that MC3T3-E1 cells cultured on MBG-NH2/EGF accelerated osteoblastic marker gene expressions, including osteopontin (Opn) and osteocalcin (Ocn). Taken together, we conclude that the osteoblast differentiation and mineralization were accelerated in MC3T3-E1 cells cultured on MBG-NH2/EGF through ERK-activated Runx2 pathway. These findings support the idea that MBG-NH2/EGF is a potential biomaterial for bone tissue repair in bone defect-related diseases.


Assuntos
Materiais Biocompatíveis/farmacologia , Regeneração Óssea/efeitos dos fármacos , Fator de Crescimento Epidérmico/farmacologia , Vidro/química , Osteoblastos/citologia , Engenharia Tecidual/métodos , Tecidos Suporte/química , Animais , Calcificação Fisiológica/efeitos dos fármacos , Adesão Celular/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Camundongos , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Porosidade
20.
World J Emerg Med ; 8(1): 5-11, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28123613

RESUMO

BACKGROUND: This meta-analysis aimed to determine whether extracorporeal cardiopulmonary resuscitation (ECPR), compared with conventional cardiopulmonary resuscitation (CCPR), improves outcomes in adult patients with cardiac arrest (CA). DATA RESOURCES: PubMed, EMBASE, Web of Science, and China Biological Medicine Database were searched for relevant articles. The baseline information and outcome data (survival, good neurological outcome at discharge, at 3-6 months, and at 1 year after CA) were collected and extracted by two authors. Pooled risk ratios (RRs) and 95% confidence intervals (CIs) were calculated using Review Manager 5.3. RESULTS: In six studies 2 260 patients were enrolled to study the survival rate to discharge and long-term neurological outcome published since 2000. A significant effect of ECPR was observed on survival rate to discharge compared to CCPR in CA patients (RR 2.37, 95%CI 1.63-3.45, P<0.001), and patients who underwent ECPR had a better long-term neurological outcome than those who received CCPR (RR 2.79, 95%CI 1.96-3.97, P<0.001). In subgroup analysis, there was a significant difference in survival to discharge favoring ECPR over CCPR group in OHCA patients (RR 2.69, 95%CI 1.48-4.91, P=0.001). However, no significant difference was found in IHCA patients (RR 1.84, 95%CI 0.91-3.73, P=0.09). CONCLUSION: ECPR showed a beneficial effect on survival rate to discharge and long-term neurological outcome over CCPR in adult patients with CA.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA