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Cell Physiol Biochem ; 50(5): 1617-1637, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30384356

RESUMO

BACKGROUND/AIMS: Heat stress could cause huge losses for Lentinula edodes in China and other Asian cultivation areas. Yet our understanding of mechanism how to defend to heat stress is incomplete. METHODS: Using heat-tolerant and heat-sensitive strains of L. edodes, we reported a combined proteome and transcriptome analysis of L. edodes response to 40 °C heat stress for 24 h. Meanwhile, the effect of LeDnaJ on the thermotolerance and IAA (indoleacetic acid) biosynthesis in L. edodes was analyzed via the over-expression method. RESULTS: The proteome results revealed that HSPs (heat shock proteins) such as Hsp40 (DnaJ), Hsp70, Hsp90 and key enzymes involved in tryptophan and IAA metabolism process LeTrpE, LeTrpD, LeTam-1, LeYUCCA were more highly expressed in S606 than in YS3357, demonstrating that HSPs and tryptophan as well as IAA metabolism pathway should play an important role in thermotolerance. Over-expression of LeDnaJ gene in S606 strains showed better tolerance to heat stress. It was also documented that intracellular IAA accumulation of S606 (8-fold up) was more than YS3357 (2-fold up), and exogenous IAA enhanced L. edodes tolerance to heat stress. CONCLUSION: Our data support the interest of LeTrpE, LeDnaJ, tryptophan and IAA could play a pivotal role in enhancing organism thermotolerance.


Assuntos
Agaricales/metabolismo , Proteínas de Choque Térmico/metabolismo , Ácidos Indolacéticos/metabolismo , Proteoma/metabolismo , Termotolerância , Transcriptoma , Agaricales/genética , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Perfilação da Expressão Gênica , Proteínas de Choque Térmico/genética , Temperatura Alta , Micélio/crescimento & desenvolvimento , Micélio/metabolismo , Isoformas de Proteínas/metabolismo , Proteoma/análise , Proteômica , Termotolerância/genética , Triptofano/metabolismo
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