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1.
Chemosphere ; 256: 127002, 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32445997

RESUMO

Pharmaceuticals and personal care products (PPCPs) has been of concerns for their potential threats to ecosystems and human's health for decades. PPCPs have been detected in water environments worldwide and have been identified in water sources and finished water. To elucidate the potential exposure of PPCPs in drinking water, this study assessed the occurrences and treatment efficiencies of PPCPs in the drinking water of Taiwan. Raw and finished water samples collected from five main drinking water treatment plants (DWTPs) in February, June, and November 2018 were analyzed. Furthermore, laboratory-scale water treatment processes were conducted to evaluate the treatment efficiencies of these chemicals. Most of the water samples from the DWTPs had a low concentration (<30 ng/L) of PPCPs. Only samples from a DWTP was observed to have higher concentration of ibuprofen (55.6 ng/L), benzophenone (92.5 ng/L), caffeine (390.5 ng/L), and diethyltoluamide (DEET) (434.9 ng/L) in raw water than others. The results of laboratory simulations indicated that the pre-chlorination process was the key step responsible for the removal of PPCPs in conventional water treatment processes, which can remove most of the hormone treatment products, parabens, oxybenzone, and acetaminophen in water sources. However, the filtration process with anthracite as a medium could remove some of the parabens (approximately 11.9%-41.2%), hormones (approximately 18.2%-44.8%), suntan lotions (37.5%-68.8%), and naproxen (30.1%) from Milli-Q water. The removal efficiencies of the aforementioned chemicals were marginally lower in raw water. However, analgesics, caffeine, and DEET cannot be removed effectively through conventional drinking water treatment.

2.
Andrologia ; : e13600, 2020 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-32364254

RESUMO

Lead (Pb) is an environmental toxicant reported to impair male reproductive system. Betaine is a natural product which has promising beneficial effects against oxidative stress. In this experimental study, we evaluated the ameliorative effect of betaine on sperm quality and oxidative stress induced by lead (Pb) in the testis of adult male mice. Sixty male Kunming mice were divided equally into four groups: control group, betaine group (1% in drinking water), lead group (100 mg kg-1  bw-1  day-1 ) and betaine + lead group. In the last group, mice were supplemented with betaine for two weeks prior to the initiation of lead treatment and concurrently during lead treatment for 3 weeks until sacrificed. Our results indicated that in the lead-administrated group, body weights together with sperm count were significantly decreased (p < .05). The numbers of abnormal sperms were found to be higher in lead-treated mice. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (Cat) were significantly reduced, while the level of malondialdehyde (MDA) content was increased in the testis tissue following lead treatment. The mRNA levels of antioxidant-related genes (SOD1, GPX1 and CAT) were significantly decreased in the lead group. Betaine enhanced these parameters in betaine + lead group. In testis histology span, Johnson score was decreased (p < .05) in lead group and co-treatment with betaine increased Johnson score significantly in betaine + lead group. These results indicate that betaine improves sperm quality and ameliorate oxidative damage in testis of mice exposed to lead.

3.
Neoplasia ; 22(5): 220-230, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32315812

RESUMO

PURPOSE: miR-5100 participates in the proliferation of lung cancer and pancreatic cancer cells, and participates in the differentiation of osteoblasts. However, the regulation of gastric cancer cells in gastric cancer cells remains unclear. EXPERIMENTAL DESIGN: The blood of patients was collected to detect the expression level of miR-5100, and the apoptosis and autophagy levels of cells were detected using western blot, flow cytometry, and confocal. At the same time, in vitro tumor formation experiments in nude mice were used to verify the results of in vitro experiments. RESULTS: The expression of miR-5100 is related to the prognosis of gastric cancer, miR-5100 can enhance the apoptosis level of gastric cancer cells and inhibit the occurrence of autophagy by targeting CAAP1. MKL1 can inhibit the apoptosis of gastric cancer cells and promote the occurrence of autophagy by targeting CAAP1. At the same time, MKL1 can also increase the expression of miR-5100. CONCLUSIONS: Our research reveals the mechanism by which the MKL1/miR-5100/CAAP1 loop regulates apoptosis and autophagy levels in gastric cancer cells, and miR-5100 is expected to become a new potential target for gastric cancer treatment.

4.
Nanoscale ; 12(10): 6096-6103, 2020 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-32129393

RESUMO

Integration of diagnostic and therapeutic components into a single coordination polymer nanoparticle is desirable for theranostic applications, but still challenging. Herein, we report the synthesis of bimetal-phenolic coordination polymer nanoparticles using gadolinium nitrate and ferrous sulphate as a metal source, and plant polyphenols (i.e., tannic acid) as an organic ligand via a metal-catechol coordination assembly process. Such coordination polymers show a tunable molar ratio of Gd/Fe and high dispersibility and stability in aqueous solution. The coordination polymers reveal composition-dependent performance for longitudinal relaxivity and photothermal conversion. The longitudinal relaxivity is positively related to the molar ratio of Gd/Fe, while the photothermal performance is negatively related to the molar ratio of Gd/Fe in the coordination polymers. The coordination polymers with an optimized molar ratio of Gd/Fe exhibit an ultra-small hydrodynamic diameter (∼23 nm), a high r1 value (9.3 mM-1 s-1) with low r2/r1 (1.26) and high photothermal conversion efficiency (η = 37%). They can be used as a contrast agent for T1-weighted magnetic resonance imaging of EMT-6 tumor bearing mice, which can effectively enhance the signals of tumors. They can also effectively suppress tumor growth via photothermal therapy. This work brings new insights for the synthesis of multifunctional coordination polymer nanoparticles and extending their potential applications in theranostics.

5.
Water Res ; 170: 115296, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31760361

RESUMO

Increased frequency and severity of extreme weather events (i.e., floods and droughts) combined with higher temperatures can threaten surface water quality and downstream drinking water production. This study characterized the effects of extreme weather events on dissolved organic matter (DOM) washout from watershed soils and the corresponding contribution to disinfection by-product (DBP) precursors under simulated weather conditions. A laboratory simulation was performed to assess the effects of temperature, drought, rainfall intensity, sea level rise, and acid deposition on the amount of DOM released from soil samples. DBP formation potentials (DBPFPs) were obtained to assess the effect of extreme weather events on DBP formation and drinking water quality. The results demonstrated that the dissolved organic carbon (DOC) and carbonaceous DBP levels increased with increasing temperature in a dry (drought) scenario. Regardless of the watershed from which a soil sample was obtained and the incubation temperature during rewetting or chlorination processes, the DOC and carbonaceous DBP levels also increased with increasing temperature. Brominated DBP formation was increased when bromide was present during the rewetting of soil, indicating the effect of sea level rise. When bromide was present during the chlorination of water for DBPFP tests, only the level of brominated DBPs increased. Acid deposition had various effects under different weather conditions. The results of heavy rainfall simulations suggested that water quality deteriorates at the beginning of an extreme rainfall event. Abundant DOM was washed out of soil, leading to a peak in the DBPFP level. The level of DOM in seepage water was less than that of the surface runoff water during rainfall. The situation was more severe when the rainfall came after a long drought and the drought-rewetting cycle effect occurred.


Assuntos
Poluentes Químicos da Água , Purificação da Água , Desinfecção , Trialometanos , Qualidade da Água
6.
J Appl Toxicol ; 40(5): 600-618, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31884710

RESUMO

Tris(2-ethylhexyl) phosphate (TEHP, CAS no. 78-42-2) is a plasticizer and a flame retardant, while di(2-ethylhexyl) phosphoric acid (DEHPA, CAS no. 298-07-7) is an oil additive and extraction solvent. Publicly-available information on repeated exposure to these two related organophosphate compounds is fragmentary. Hence, adult male and female Fischer rats were exposed to TEHP (300, 1000 and 3000 mg/kg body weight [BW]/day) or DEHPA (20, 60 and 180 mg/kg BW/day) by gavage for 28 consecutive days, to assess and compare their toxicities. Although significantly impaired BW gains and evidence of TEHP enzymatic hydrolysis to DEHPA were observed only in males, exposures to the highest TEHP and DEHPA doses often resulted in similar alterations of hematology, serum clinical chemistry and liver enzymatic activities in both males and females. The squamous epithelial hyperplasia and hyperkeratosis observed in the non-glandular forestomach of rats exposed to the middle and high DEHPA doses were most likely caused by the slightly corrosive nature of this chemical. Although tubular degeneration and spermatid retention were observed only in the testes of males exposed to the highest TEHP dose, numerous periodic acid-Schiff stained crystalline inclusions were observed in testis interstitial cells at all TEHP dose levels. No-observed-adverse-effect levels for TEHP and DEHPA are proposed, but the lower serum pituitary hormone levels resulting from TEHP and DEHPA exposures and the perturbations of testicular histology observed in TEHP-treated males deserve further investigation. Improved characterization of the toxicity of flame retardants will contribute to better informed substitution choices for legacy flame retardants phased-out over health concerns.

7.
J Hazard Mater ; 389: 121873, 2020 05 05.
Artigo em Inglês | MEDLINE | ID: mdl-31862351

RESUMO

Arbuscular mycorrhizal fungi (AMF) and earthworms independently enhance plant growth, heavy metal (HM) tolerance, and HM uptake, thus they are potential key factors in phytoremediation. However, few studies have investigated their interactions in HM phytoextraction by hyperaccumulators. This study highlights the independent and interactive effects of earthworms and AMF on Solanum nigrum. Plants inoculated with either AMF or earthworms exhibited ameliorated growth via enhancement of productivity, metal tolerance, and phosphorus (P) acquisition. Co-inoculation with both had more pronounced effects on plant biomass and P acquisition in shoots, but not in roots, and in Cd-polluted soils it significantly promoted (P < 0.05) shoot biomass (20.7-134.6 %) and P content (20.4-112.0 %). AMF and earthworms increased Cd accumulation in plant tissues, but only AMF affected Cd partitioning between shoots and roots. Although AMF decreased root-to-shoot translocation of Cd at high Cd levels, this was counterbalanced by earthworms. Both AMF and its combination with earthworms enhanced Cd phytoavailability by altering Cd chemical fractions and decreasing pH. Co-inoculation increased Cd removal amounts up to 149.3 % in 120 mg kg-1 Cd-spiked soils. Interactions between the two organisms were synergistic in Cd phytoextraction. Thus, earthworm-AMF-plant symbiosis potentially plays an essential role in phytoremediation of HM-polluted soils.

8.
Huan Jing Ke Xue ; 40(9): 3962-3972, 2019 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854858

RESUMO

Odor pollution has a high complaint rate with strong public concern, and industrial production is an important source of this type of pollution in China. To understand odor pollution in industrial parks and to protect the safety of work environments, samples were collected from 14 industrial odor sources and then were analyzed for odorous volatile organic compounds (VOCs) and odor concentration. Based on the field data, the main compounds causing odor were assessed and identified. The cancer and non-cancer risk of odor exposure were correspondingly estimated by the health risk model. These following results were noted. ①The substances discharged from 14 sources were the same, but the content varied greatly. Alkanes and alkenes are the major odorous compounds of fibers and batteries manufacturing and in the synthesis of hydrocarbons, anhydrides, esters, and solvents. Benzene and benzene series in waste gases from refineries, purified terephthalic acid (PTA), and latex sources were the highest. Esters are the main pollutants emitted from activated carbon processing, resin synthesis, and spraying. Carbonyl compounds and sulfides are the main exhaust gases from ceramic manufacturing and additive synthesis. ②Exhaust gases from 14 sources caused strong irritation. The synthesis of lubricating oil additives and latex sources result in severe olfactory stimulation. Ethyl mercaptan, ethyl sulfide, n-butanol, and toluene were the major odorous compounds of lubricating oil additives sources. Styrene, propylbenzene, cumene, butyl acrylate, and 1,3-butadiene were the major odorous compounds of latex sources. ③The carcinogenic risk levels for 14 sources ranged from 3.06×10-7 to 1.06×10-2, expressed as life cancer risk (LCR). Refinery, PTA, ester, and latex sources had the highest carcinogenic risk among the 14 emission sources. The non-carcinogenic risk levels for the 14 sources ranged from 0.02 to 51.66, expressed as hazard index (HI). The total HI of latex synthesis, ester synthesis, petroleum refining, PTA synthesis, and fiber manufacturing has certain non-carcinogenic health risks. Factory boundaries for latex, anhydrides synthesis, and resin synthesis sources have potential carcinogenic risk.


Assuntos
Poluentes Atmosféricos , Saúde Ambiental , Poluentes Ambientais , Odorantes , Compostos Orgânicos Voláteis , China , Monitoramento Ambiental , Medição de Risco
9.
World J Clin Cases ; 7(20): 3194-3201, 2019 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-31667169

RESUMO

BACKGROUND: Mesenchymal tumors such as perivascular epithelioid cell neoplasm (PEComa) and inflammatory pseudotumor-like follicular dendritic cell sarcoma (IPT-like FDC sarcoma) are relatively uncommon in the liver and are particularly rare in the caudate lobe. The clinical manifestations and available imaging tests lack specificity for hepatic mesenchymal tumors. To the best of our knowledge, no caudate PEComa or IPT-like FDC sarcoma has been completely resected by laparoscopy. The standard laparoscopic technique, surgical approaches, and tumor margins for potentially malignant or malignant caudate mesenchymal tumors are still being explored. AIM: To assess both the safety and feasibility of laparoscopic resection for rare caudate mesenchymal neoplasms. METHODS: Eleven patients who underwent isolated caudate lobe resection from 2003 to 2017 were identified from a prospective database. Three consecutive patients with rare caudate mesenchymal tumors underwent laparoscopic resection. Patient demographic data, intraoperative parameters, and postoperative outcomes were assessed and compared with the open surgery group. RESULTS: All procedures for the three resection patients with caudate mesenchymal tumors were completed using a total laparoscopic technique by two different approaches. The average operative time was 226 min, and the estimated blood loss was 133 mL. The average length of postoperative hospital stay was 6.3 ± 0.3 d for the laparoscopy group and 15.5 ± 2.3 d for the open surgery group (P < 0.05). There were no perioperative complications or patient deaths in this series. CONCLUSION: Laparoscopic isolated caudate lobe resection for rare mesenchymal neoplasms is a feasible and curative surgical option in selected patients.

11.
ACS Appl Mater Interfaces ; 11(38): 35060-35067, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31469272

RESUMO

Metal oxide hollow spheres (MOHSs) with multicomponent metal elements exhibit intriguing properties due to the synergistic effects of different components. However, it remains a great challenge to develop a general method to synthesize multicomponent MOHSs due to the different hydrolysis and condensation rates of precursors for different metal oxides. Herein, we demonstrate a general strategy for the controllable synthesis of MOHSs with up to five metal elements by decomposition of metal-phenolic coordination polymers (MPCPs), which are prepared by chelation of tannic acid with various metal ions. After calcination to burn out the organic component and induce heterogeneous contraction of MPCPs, a series of MOHSs with multishell structure, high specific surface area (55-171 m2/g), and crystalline mesoporous framework are synthesized, including binary (Fe-Co, Ni-Zn, and Ni-Co oxides), ternary (Ni-Co-Mn and Ni-Co-Zn oxides), and quinary (Ni-Co-Fe-Cu-Zn oxides) MOHSs. The gas sensing nanodevices based on quinary MOHSs show much higher response (10.91) than those based on single component toward 50 ppm of ethanol at 80 °C with the response/recovery time of 85/160 s. The quinary oxides sensor also displays high selectivity toward ethanol against other interfering gases (e.g., methanol, formadehyde, toluene, methane, and hydrogen) and long-term stability (∼94.0% after 4 weeks), which are extremely favorable for practical applications.

12.
Oxid Med Cell Longev ; 2019: 3187972, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31285783

RESUMO

Mastitis has severely affected the cattle industry worldwide and has resulted in decreased dairy production and cattle reproduction. Although prevention and treatment methods have been implemented for decades, cattle mastitis is still an intractable disease. Sirtuin 7 (SIRT7) is an NAD+-dependent deacetylase that is involved in various biological processes, including ribosomal RNA synthesis and protein synthesis, DNA damage response, metabolism, and tumorigenesis. However, whether SIRT7 participates in inflammation remains unknown. Our results revealed that SIRT7 is downregulated in tissue samples from mastitic cattle. Therefore, we isolated dairy cow mammary epithelial cells (DCMECs) from breast tissues and developed an in vitro model of lipopolysaccharide- (LPS-) induced inflammation to examine SIRT7 function and its potential role in inflammation. We showed that SIRT7 was significantly downregulated in LPS-treated DCMECs. SIRT7 knockdown significantly increased the LPS-stimulated production of inflammatory mediators, like reactive oxygen and nitric oxide, and upregulated TAB1 and TLR4. In addition, SIRT7 knockdown significantly increased the phosphorylation of TAK1 and NF-κBp65 in LPS-treated DCMECs. Moreover, SIRT7 knockdown promoted the translocation of NF-κBp-p65 to the cell nucleus and then increased the secretion of inflammatory cytokines (IL-1ß and IL-6). In contrast, SIRT7 overexpression had the opposite effects when compared to SIRT7 knockdown in LPS-treated DCMECs. In addition, SIRT7 overexpression attenuated LPS-induced DCMEC apoptosis. Taken together, our results indicate that SIRT7 can suppress LPS-induced inflammation and apoptosis via the NF-κB signaling pathway. Therefore, SIRT7 may be considered as a potential pharmacological target for clinical mastitis therapy.


Assuntos
Inflamação/induzido quimicamente , Inflamação/metabolismo , Lipopolissacarídeos/toxicidade , Sirtuínas/metabolismo , Animais , Bovinos , Células Cultivadas , Ensaio de Imunoadsorção Enzimática , Feminino , Interleucina-1beta/metabolismo , Interleucina-6/metabolismo , Glândulas Mamárias Animais/metabolismo , Mastite/metabolismo , NF-kappa B/metabolismo , Óxido Nítrico/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/genética , Transdução de Sinais/fisiologia , Sirtuínas/genética
13.
Onco Targets Ther ; 12: 3977-3989, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31190894

RESUMO

Purpose: HER2-positive breast cancer (BC) achieving pathological complete remission (pCR) after neoadjuvant therapy (NAT) had a superior disease outcome. Dysmetabolism and stimulation of insulin-like growth factor 1 (IGF-1)-axis would increase BC risk, but we are lacking data for their association with pCR in HER2-positive+ BC. We aim to evaluate the pCR predictive value of above factors in HER2-positive BC patients receiving NAT. Patients and methods: HER2-positive BC patients receiving NAT ± trastuzumab were retrospectively included between January 2013 and December 2016. Data were compared between baseline at biopsy and surgery. Median value of IGF-1 expression was used as cutoff value to classify patients into low or high group. pCR was defined as no residual invasive carcinoma in breast and axilla. Results: Overall, 101 patients were included. Metabolic syndrome was diagnosed in 29 (28.71%) with an average of 1.71±1.51 metabolic disorders at baseline, significantly increased after NAT (2.12±1.54, P<0.001). Lipid metabolism factors, including triglycerides, TC, HDL-C and LDL-C significantly worsened after NAT (all P<0.05). Average post-NAT IGF-1 was 196.14±86.03 ng/mL (vs preNAT 186.41±75.03 ng/mL, P=0.182). pCR was achieved in 29 (28.71%) patients. pCR rate was 40.00% and 17.65% for those with low or high preIGF-1 level (P=0.013). Multivariate analysis found that low IGF-1 expression, but not any other metabolic variable, was significantly associated with higher pCR rate in whole population (OR: 3.83, 95%CI: 1.32-11.11, P=0.014) or in patients receiving NAT + trastuzumab (OR: 3.93, 95%CI: 1.13-13.63, P=0.031). With a median follow-up of 29.03 (range: 10.42-56.98) months, IGF-1 level was not associated with overall survival (P=0.328) or disease-free survival (P=0.288). Conclusion: Low IGF-1 level was related with higher pCR rate in HER2-positive BC patients receiving NAT, which deserves further clinical evaluation.

14.
Can J Gastroenterol Hepatol ; 2019: 1208614, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31183338

RESUMO

Objective: Portal hypertension is a major complication of decompensated cirrhosis. In China, modified Hassab's and Sugiura procedure are the two major methods of nonshunting surgery. This study aims to compare the efficacy and safety of the two procedures for portal hypertension. Method: Between January 1994 and December 2009, 172 elective patients diagnosed with decompensated cirrhosis with significant hypersplenism adopted elective splenectomy for hypersplenism, and also modified Hassab's (n = 91) or Sugiura (n = 81) procedure was additionally performed to reduce the risk of variceal bleeding. Postoperative mortality and morbidity data were collected, and a retrospectively comparative analysis was conducted. Results: All of the patients were treated successfully without death during operation, and no variceal bleeding occurred during hospitalization. There were 4 (4.4%) deaths in Hassab's group and 3 (3.7%) deaths in Sugiura group postoperatively (P > 0.05). During follow-up, the survival rate was 90.2%, 82.42%, and 71.43% in Hassab's group and 96.29%, 81.48%, and 75.31% in Sugiura group in 1, 3, and 5 years (P > 0.05). There were 22/71 and 12/63 patients in each groups who suffered no deadly variceal bleeding (P = 0.11). Bleeding related death and no bleeding related death occurred in 7/23 and 3/13 patients in each group (P = 0.26 and 0.14, respectively). Conclusion: Elective splenectomy combined with modified Sugiura procedure seemed to be associated with a reduced trend of no deadly variceal bleeding compared with Hassab's procedure. As statistical significance was not found, further large scale and prospective study was warranted.

15.
Med Sci Monit ; 25: 3605-3616, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-31091223

RESUMO

BACKGROUND Based on the extensive biological effects of melatonin (MLT), it is beneficial to increase the MLT content in the bodies of animals at a specific physiological stage. This study was conducted to investigate the effect of a diet supplemented with rumen-protected (RP) 5-hydroxytryptophan (5-HTP) on the pineal gland and intestinal tract MLT synthesis of sheep. MATERIAL AND METHODS Eighteen Kazakh sheep were assigned randomly to 3 diet groups: control group (CT, corn-soybean meal basal diet), CT+111 group (111 mg/kg BW RP 5-HTP), and CT+222 group (222 mg/kg BW RP 5-HTP). The gene expressions of aromatic amino acid decarboxylase (AADC), arylalkylamine N-acetyltransferase (AA-NAT), hydroxyindole-O-methyltransferase (HIOMT), monoamine oxidase A (MAOA), and the intermediates of MLT synthesis were observed from the pineal gland and intestinal tract by the reverse transcription (RT)-PCR method. The 5-HTP, 5-HT, N-acetylserotonin (NAS), MLT, and 5-hydroxyindole acetic acid (5-HIAA) contents in the pineal gland and intestinal tract were analyzed by ultra-high-performance liquid chromatography-tandem mass spectrometry. RESULTS The study showed that the pineal gland HIOMT expression (P<0.05), MLT (P<0.05) and 5-HIAA (P<0.05) levels in the 222 mg/kg group significantly increased compared to those in the CT and CT+111 mg/kg groups. In addition, the AADC (P<0.01) and AA-NAT (P<0.05) gene expression levels in the duodenum and jejunum were increased by the supplementation of RP 5-HTP. CONCLUSIONS Rumen-protected 5-hydroxytryptophan promoted melatonin synthesis in the pineal gland and intestinal tract during the natural light period.


Assuntos
5-Hidroxitriptofano/farmacologia , Melatonina/metabolismo , 5-Hidroxitriptofano/metabolismo , Acetilserotonina O-Metiltransferasa , Animais , Descarboxilases de Aminoácido-L-Aromático , Arilalquilamina N-Acetiltransferase , Peso Corporal , Ritmo Circadiano , Suplementos Nutricionais , Ácido Hidroxi-Indolacético , Mucosa Intestinal/efeitos dos fármacos , Intestinos/efeitos dos fármacos , Melatonina/biossíntese , Melatonina/farmacologia , Glândula Pineal/efeitos dos fármacos , Rúmen/metabolismo , Ovinos
16.
J Pharm Biomed Anal ; 170: 285-294, 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-30951994

RESUMO

In the face of the many shortcomings of conventional organic solvents in the age of green chemistry, deep eutectic solvents (DESs) appear under the spotlight of natural product extraction because of its outstanding advantages. In this study, the extraction of six compounds from Flos Sophorae Immaturus (FSI) with DES-5 (choline chloride/1,4-butanediol) as its topgallant solvent was determined by screening nine DESs. After single factor test and BBD experiment, the optimum conditions of deep eutectic solvent-based microwave-assisted extraction (DES-MAE) were: choline chloride/1,4-butanediol (molar ratio of 1:2) and water content (25%, v/v), time 20 min, microwave power 600 W, temperature 62 ℃, liquid/solid ratio 26 mL/g. The extraction yields of rutin, nicotiflorin, narcissin, quercetin, kaempferol and isorhamnetin were 116.78, 15.01, 23.85, 27.59, 3.09 and 3.33 mg/g, respectively. The kinetic experiment results showed that DES-MAE has significant advantages in the extraction of six compounds. The experimental results showed that DES-MAE could obtain higher yields of target components in a short time than other methods (DES-HRE, DES-UAE and Ethanol-MAE). In addition, the target components were separated from the DES extraction solution, and the recoveries of the target compounds were in the range of 75.5%-84.1%. Therefore, this paper provides a strategy for extraction and separation, the aim of which is to obtain flavonoids from FSI efficiently. Meanwhile, this study can also be used as an alternative to the traditional methods for obtaining bioactive components from plant sources in biochemistry, food industry and pharmaceutical fields.


Assuntos
Flavonoides/química , Extratos Vegetais/química , Solventes/química , Flavonóis/química , Flores/química , Quempferóis/química , Micro-Ondas , Fenóis/química , Quercetina/análogos & derivados , Quercetina/química , Rutina/química , Água/química
17.
Breast Cancer Res Treat ; 175(3): 567-578, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30937657

RESUMO

PURPOSE: In early stage, ERα-positive breast cancer, concurrent use of endocrine therapy and chemotherapy has not been shown to be superior to sequential use. We hypothesized that genetic biomarkers can aid in selecting patients who would benefit from chemo-endocrine therapy. Our previous studies revealed that ZNF423 is a transcription factor for BRCA1 and an intronic single nucleotide polymorphism (SNP) in ZNF423, rs9940645, determines tamoxifen response. Here, we identified mitosis-related genes that are regulated by ZNF423 which led us to investigate taxane response in a rs9940645 SNP- and tamoxifen-dependent fashion. METHODS: The Cancer Genome Atlas (TCGA) breast cancer dataset was used to identify genes correlated with ZNF423. Quantitative reverse transcription PCR, chromatin immunoprecipitation, and luciferase reporter assays were used to validate the gene regulation. We used CRISPR/Cas9 to engineer paired ZR-75-1 cells which differ only in ZNF423 rs9940645 SNP genotype to test SNP-dependent phenotypes including cell cycle and cell viability. We validated our findings in an additional two breast cancer cell lines, Hs578T-ERα and HCC1500. RESULTS: Mitosis-related genes VRK1 and PBK, which encode histone H3 kinases, were experimentally validated to be regulated by ZNF423. ZNF423 knockdown decreased VRK1 and PBK expression and activity. Additionally, ZNF423 knockdown enhanced docetaxel-induced G2/M arrest and cytotoxicity through VRK1 or PBK regulation. Lastly, cells carrying the rs9940645 variant genotype had increased G2/M arrest and decreased cell viability when treated with docetaxel in combination with estradiol and 4-OH-TAM. CONCLUSIONS: We identified ZNF423 regulated genes involved in the G2/M phase of the cell cycle. 4-OH-TAM sensitized ERα-positive breast cancer cells to docetaxel in a ZNF423 SNP-dependent manner. Our findings suggest that patients with rs9940645 variant genotype may benefit from concurrent tamoxifen and docetaxel. This would impact a substantial proportion of patients because this SNP has a minor allele frequency of 0.47.


Assuntos
Antineoplásicos/farmacologia , Neoplasias da Mama/genética , Docetaxel/farmacologia , Estradiol/farmacologia , Receptor alfa de Estrogênio/genética , Proteínas/genética , Tamoxifeno/análogos & derivados , Neoplasias da Mama/tratamento farmacológico , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Sinergismo Farmacológico , Receptor alfa de Estrogênio/metabolismo , Feminino , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/genética , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Quinases de Proteína Quinase Ativadas por Mitógeno/genética , Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo , Polimorfismo de Nucleotídeo Único , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/metabolismo , Proteínas/metabolismo , Tamoxifeno/farmacologia
18.
Oncol Lett ; 17(5): 4514-4520, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30988817

RESUMO

Metadherin (MTDH) is a protein that is also named astrocyte elevated gene-1, and is highly expressed in a number of different tumor tissues. Although the expression of MTDH is associated with tumor invasion and recurrence, the expression of this protein in perihilar cholangiocarcinoma (PCCA) and its clinical use have not yet been investigated. In the present study, the expression of MTDH in patients with PCCA was investigated in order to determine its clinicopathological use. An immunohistochemical method was used to detect MTDH expression and the epithelial-mesenchymal transition markers E-cadherin and vimentin in 66 cases of PCCA. In addition to the expression of MTDH, the clinical and pathological data and the postoperative outcomes were analyzed. The MTDH positive expression rate was 48.5% (32/66) in PCCA. A significantly higher MTDH expression level was identified in the poor tumor differentiation group compared with the well differentiation group (P=0.007). In the positive lymph node metastasis group, a significantly higher MTDH expression level was revealed compared with the negative lymph node metastasis group (P=0.023). No association was noted with regard to the expression of MTDH and the variables age, sex, tumor diameter, tumor grade and tumor classification stage. Positive MTDH expression was significantly associated with high vimentin expression (P=0.037) compared with negative vimentin expression and inversely associated with positive E-cadherin expression compared with negative E-cadherin expression (P=0.030). Survival analysis suggested that the high MTDH expression group was associated with a worse overall survival (OS) rate and recurrence free survival (RFS) rate compared with the low MTDH expression group (P<0.001 and P=0.01, respectively). Cox regression analysis indicated that the Tumor-Node-Metastasis, surgery margin and high MTDH expression were independent OS and RFS factors for PCCA. MTDH expression may serve an important function in PCCA tumor growth and metastasis. Targeting MTDH may have important therapeutic applications for patients with PCCA.

19.
Molecules ; 24(8)2019 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-31027249

RESUMO

Fibrous hydrogel scaffolds have recently attracted increasing attention for tissue engineering applications. While a number of approaches have been proposed for fabricating microfibers, it remains difficult for current methods to produce materials that meet the essential requirements of being simple, flexible and bio-friendly. It is especially challenging to prepare cell-laden microfibers which have different structures to meet the needs of various applications using a simple device. In this study, we developed a facile two-flow microfluidic system, through which cell-laden hydrogel microfibers with various structures could be easily prepared in one step. Aiming to meet different tissue engineering needs, several types of microfibers with different structures, including single-layer, double-layer and hollow microfibers, have been prepared using an alginate-methacrylated gelatin composite hydrogel by merely changing the inner and outer fluids. Cell-laden single-layer microfibers were obtained by subsequently seeding mouse embryonic osteoblast precursor cells (MC3T3-E1) cells on the surface of the as-prepared microfibers. Cell-laden double-layer and hollow microfibers were prepared by directly encapsulating MC3T3-E1 cells or human umbilical vein endothelial cells (HUVECs) in the cores of microfibers upon their fabrication. Prominent proliferation of cells happened in all cell-laden single-layer, double-layer and hollow microfibers, implying potential applications for them in tissue engineering.


Assuntos
Hidrogéis/química , Microfluídica/métodos , Engenharia Tecidual/métodos , Animais , Células Endoteliais da Veia Umbilical Humana/citologia , Humanos , Camundongos
20.
J Colloid Interface Sci ; 544: 241-248, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30851685

RESUMO

The conversion of plant polyphenol to multi-functional nanoporous carbon spheres (NCSs) is desirable and challenging. Tannic acid (TA), as one kind of plant polyphenol, has been regarded as one of potential renewable carbon sources for nanoporous carbon materials. However, the nanoporous carbon materials derived from TA usually show low surface area and irregular morphology, which partially limit their broad applications. Herein, we demonstrate the successful synthesis of NCSs with high surface area (up to 2221 m2/g) and uniform diameter (∼120 nm) by direct carbonization of zinc-phenolic coordination polymer using TA as a carbon source. The zinc contents play an important role in tailoring the porosity of NCSs. The specific surface area of NCSs can be adjusted in the range of 527-2221 m2/g as the zinc contents in the coordination polymers changed from 0 to 8.6 wt%. To verify the multi-functions of NCSs, such carbon spheres are further used as a sensing platform for the analysis of nucleic acid variants with high selectivity and low limit of detection, and electrode material for supercapacitor with high specific capacitance, good capacitance retention, and excellent cyclic stability.


Assuntos
Carbono/química , Complexos de Coordenação/química , Nanoporos/ultraestrutura , Polímeros/química , Taninos/química , Zinco/química , Técnicas Biossensoriais/métodos , Capacitância Elétrica , Técnicas Eletroquímicas/métodos , Eletrodos , Limite de Detecção , Tamanho da Partícula , Porosidade , Propriedades de Superfície
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