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1.
Phytomedicine ; 62: 152948, 2019 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-31129431

RESUMO

BACKGROUND: Huangqi decoction (HQD), a classic traditional herbal medicine, has been used for liver fibrosis, but its effect on intrahepatic chronic cholestatic liver injury remains unknown. PURPOSE: In the present study, we investigated the hepatoprotective effect of HQD and the underlying molecular mechanisms in 3, 5-diethoxycarbonyl-1, 4-dihydroxychollidine (DDC)-induced chronic cholestatic mice. METHODS: The DDC-induced cholestatic mice were administrated HQD for 4 or 8 weeks. Serum biochemistry and morphology were investigated. The serum and liver bile acid (BA) levels were detected by ultra performance liquid chromatography-tandem mass spectrometry. The liver expression of BA metabolizing enzymes and transporters, and inflammatory and fibrotic markers was measured by real-time polymerase chain reaction, western blotting, and immunohistochemistry. RESULTS: HQD treatment for 4 or 8 weeks ameliorated DDC-induced liver injury by improving impaired hepatic function and tissue damage. HQD treatment for 8 weeks further decreased the liver expression of cytokeratin 19, tumor growth factor (TGF)-ß, collagen I, and α-smooth muscle actin, and ameliorated ductular reaction and liver fibrosis. HQD markedly decreased the accumulation of serum and liver BA. The expression of BA-metabolizing enzymes, cytochrome P450 2b10 and UDP glucuronosyltransferase 1 A1, and multidrug resistance-associated protein 2, Mrp3, and Mrp4 involved in BA homeostasis was increased by 4 weeks of HQD treatment. The expression of BA uptake transporter Na+-taurocholate cotransporting polypeptide was decreased and that of Mrp4 was increased after 8 weeks of HQD treatment. Nuclear factor-E2-related factor-2 (Nrf2) was remarkably induced by HQD treatment. Additionally, HQD treatment for 8 weeks decreased the liver expression of inflammatory factors, interleukin (IL)-6, IL-1ß, tumor necrosis factor-α, monocyte chemoattractant protein-1, and intracellular adhesion molecule-1. HQD suppressed the nuclear factor (NF)-κB pathway. CONCLUSION: HQD protected mice against chronic cholestatic liver injury and biliary fibrosis, which may be associated with the induction of the Nrf2 pathway and inhibition of the NF-κB pathway, ameliorating BA-stimulated inflammation.

2.
Biomed Pharmacother ; 112: 108701, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30818137

RESUMO

Natural bear bile has been used for liver disease in East Asia for thousands of years. However, its use has restrictions. In the current study, the therapeutic effects and potential mechanisms of cultured bear bile powder (CBBP) against hepatic fibrosis were evaluated in a dimethylnitrosamine (DMN)-induced rat model. CBBP treatment significantly improved DMN-induced hepatic necrosis and inflammatory infiltration. Additionally, CBBP remarkably alleviated the increased hepatic collagen content and expression of alpha-smooth muscle actin. Serum metabolomics revealed that 14 serum metabolites, including docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were decreased in DMN-treated rats, which was reversed by CBBP. Pathway analyses revealed that the main metabolic pathways affected by CBBP were related to fatty acid biosynthesis and metabolism, and biosynthesis of unsaturated fatty acids. EPA and DHA are ligands of peroxisome proliferator activated receptors (PPARs). CBBP treatment significantly stimulated liver mRNA and protein expression of PPARα and PPARγ. CBBP also markedly increased liver expression of PPARα target genes, which are involved in fatty acid ß-oxidation, and down-regulated IL-6, a downstream inflammatory gene of PPARγ. In conclusion, CBBP has the potential to attenuate liver fibrosis and its mechanism involves the promotion of the liver expression of PPARα and PPARγ. Our results may help in the development of a novel substitute for bear bile and therapeutic strategies for fibrotic liver diseases.


Assuntos
Bile/metabolismo , Citoproteção/efeitos dos fármacos , Dimetilnitrosamina/toxicidade , Cirrose Hepática Experimental/induzido quimicamente , Cirrose Hepática Experimental/metabolismo , Animais , Bile/química , Citoproteção/fisiologia , Relação Dose-Resposta a Droga , Cirrose Hepática/induzido quimicamente , Cirrose Hepática/metabolismo , Cirrose Hepática/prevenção & controle , Cirrose Hepática Experimental/prevenção & controle , Distribuição Aleatória , Ratos , Ratos Wistar , Ursidae
3.
Res Microbiol ; 169(2): 115-126, 2018 Feb - Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29122672

RESUMO

Soil microorganisms play a crucial role in cycling soil nutrients and providing organic nutrients for plant growth and development. Fertilisation balances soil fertility and quality, and affects soil microbial communities. Fertilisation is a frontier subject in agricultural and environmental sciences. Here we showed that the application of high-carbon basal fertiliser treatment could improve the tobacco yield and quality when compared to chemical fertiliser, high-carbon basal fertiliser and mixed high-carbon chemical fertiliser. The potential reason is that different fertiliser treatments influence soil fertility, such as nitrogen, phosphorus, and other contents, besides soil organic matter. Further experiments revealed that populations of bacteria, fungi and actinomycetes fluctuated during tobacco development under different fertilisation treatments. Then we performed high-throughput sequencing of the 16S rRNA gene, and the results showed that the fertilisation treatments had significant effects on the microbial community, particularly within the finer taxonomic divisions or non-dominant taxa. Moreover, proteobacteria and fungal genera had significantly different relative abundances during tobacco growth under various tobacco developmental stages and fertilisation treatments. These results indicated that mixed high-carbon chemical fertiliser could improve soil fertility by influencing the soil microorganism, and that the fertilisation treatments impacted on the structure and composition of the microbial community, and especially the diversity of non-dominant taxa. However, more studies are needed to confirm their reliability.


Assuntos
Bactérias/isolamento & purificação , Carbono/análise , Fertilizantes/análise , Fungos/isolamento & purificação , Microbiologia do Solo , Tabaco/crescimento & desenvolvimento , Bactérias/classificação , Bactérias/genética , Bactérias/metabolismo , Biodiversidade , Carbono/metabolismo , Fungos/classificação , Fungos/genética , Fungos/metabolismo , Fósforo/análise , Fósforo/metabolismo , Solo/química , Tabaco/microbiologia
4.
Oncotarget ; 8(57): 97137-97152, 2017 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-29228599

RESUMO

This study explored the effects of chicken bile powder (CBP), a 2000-year-old Chinese medicine, on α-naphthyl isothiocyanate (ANIT)-induced intrahepatic cholestasis in mice. CBP treatment for 14 days significantly ameliorated ANIT-induced changes in serum alanine aminotransferase, aspartate aminotransferase, bile acids, bilirubin, γ-glutamyl transpeptidase, alkaline phosphatase, and liver tissue morphology. Serum metabolomics showed changes in 24 metabolites in ANIT-exposed mice; 16 of these metabolites were reversed by CBP treatment via two main pathways (bile acid biosynthesis and arachidonic acid metabolism). Additionally, CBP administration markedly increased fecal and biliary bile acid excretion, and reduced total and hydrophobic bile acid levels in the livers of cholestatic mice. Moreover, CBP increased liver expression of bile acid efflux transporters and metabolic enzymes. It also attenuated ANIT-induced increases in hepatic nuclear factor-κB-mediated inflammatory signaling, and increased liver expression of the nuclear farnesoid X receptor (FXR) in cholestatic mice. CBP also activated FXR in vitro in HEK293T cells expressing mouse Na+-taurocholate cotransporting polypeptide. It did not ameliorate the ANIT-induced liver injuries in FXR-knockout mice. These results suggested that CBP provided protection from cholestatic liver injury by restoring bile acid homeostasis and reducing inflammation in a FXR-dependent manner.

5.
J Clin Lab Anal ; 29(4): 289-93, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24849556

RESUMO

BACKGROUND: This study aimed to determine the relationship between hematocrit (HCT) levels and cardiovascular risk factors in a community-based population of middle-aged adults. METHODS: From April 2011 to February 2012, a total of 1,884 middle-aged adults were selected from a community-based population in China. Blood and urine samples were collected for routine blood and urine tests, and measurement of plasma glucose and lipid levels. Baseline information including traditional cardiovascular risk factors was obtained by standard questionnaire to analyze. We evaluated the distribution of the HCT values for middle-aged adults with or without cardiovascular risk factors. There were 548 males and 1,336 females in this study. The mean age of all subjects was 54.7 ± 6.7 years. There were 1,209 subjects with risk factors and 675 without risk factors. RESULTS: The HCT levels in subjects with risk factors were higher than those without risk factors (P = 0.005). According to a simplified tool for evaluation of the 10-year risk of ischemic cardiovascular diseases (CVDs) in Chinese populations, all subjects were divided into four groups: the ultralow-risk group (1,367, 72.6%), low-risk group (232, 12.3%), intermediate-risk group (201, 10.7%), and high-risk/ultrahigh-risk group (84, 4.4%). Compared with HCT levels in the ultralow-risk group, significant differences were found in the low-risk, intermediate-risk, and high-risk/ultrahigh-risk groups (all P < 0.05). CONCLUSION: Our results indicate that elevated HCT levels may be positively associated with cardiovascular risk factors. Thus, the combination of HCT values and cardiovascular risk factors may enable early diagnosis of CVDs.


Assuntos
Doenças Cardiovasculares/sangue , Hematócrito , Características de Residência , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Risco
6.
Spectrochim Acta A Mol Biomol Spectrosc ; 134: 406-12, 2015 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-25025313

RESUMO

A series of heteroleptic cyclometalated Ir (III) complexes for OLEDs application have been investigated theoretically to explore their electronic structures and spectroscopic properties. The geometries, electronic structures, and the lowest-lying singlet absorptions and triplet emissions of (piq)2Ir(acac) (labeled 1) and theoretically designed models (piq)2Ir(dpis) (labeled 2), (4Fpiq)2Ir(dpis) (labeled 3), (4F5M-piq)2Ir(dpis) (labeled 4), (4,5-2F-piq)2Ir(dpis) (labeled 5) and (5-F-piq)2Ir(dpis) (labeled 6) were investigated with density functional theory (DFT)-based approaches, where, piq=1-phenylisoquinolato, acac=acetylacetonate and dpis=diphenylimidodisilicate. Their structures in the ground and excited states have been optimized at the DFT/B3LYP/LANL2DZ and TDDFT/B3LYP/LANL2DZ levels, and the lowest absorptions and emissions were evaluated at B3LYP and M062X level of theory, respectively. Furthermore, the energy-transfer mechanism of these complexes also be analyzed here, and the result shown that the complexes 1-6 are having the low efficiency roll-off property. Except that, the oscillator strength analyze shown that the complexes 2-6, which were designed by theory, are suitable for OLED since their high oscillator strength property.


Assuntos
Complexos de Coordenação/química , Irídio/química , Elétrons , Modelos Moleculares , Teoria Quântica
7.
Obes Surg ; 25(2): 199-208, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25103403

RESUMO

BACKGROUND: Although a few studies have been reported on predictive factors of postoperative diabetes remission, the conclusions remain inconsistent. This meta-analysis aimed to assess the preoperative clinical factors for type 2 diabetes mellitus (T2DM) remission after bariatric surgery. METHODS: The Cochrane Library, PubMed, MEDLINE, Embase, and CINAHL databases were searched. All human studies published in English between 1 January 1992 and 1 September 2013 reporting on the parameters of interest were included. RESULTS: In total, 15 studies involving 1,753 bariatric surgery patients were selected. Analyses were performed separately for the parameters of interest. T2DM remission was observed to be negatively correlated with age, diabetes duration, insulin use, and HbA1c levels. Baseline body mass index (BMI) and C-peptide levels were positively associated with the remission rate in Asian patients. However, there was no significant association between gender and remission rate. CONCLUSIONS: Patients with younger age, short diabetes duration, better glucose control, and better ß cell function were more likely to achieve T2DM remission after bariatric surgery. However, further randomized controlled trials with uniform remission criteria should be conducted to provide more reliable evidence.


Assuntos
Diabetes Mellitus Tipo 2/cirurgia , Adulto , Fatores Etários , Cirurgia Bariátrica , Glicemia/análise , Diabetes Mellitus Tipo 2/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Indução de Remissão , Fatores de Tempo
8.
Mol Biol Rep ; 41(12): 7923-8, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25179225

RESUMO

This study aimed to investigate the effects of acetaldehyde (AA) and L-carnitine (LC) on morphology and enzyme activity of myocardial mitochondria in rats. Sixty-five Wistar rats were randomly divided into 4 groups: the control group (n = 20), the AA low-dose group (n = 15), the AA high-dose group (n = 15) and the AA + LC group (n = 15). Different doses (110 mg/kg and 220 mg/kg) AA was injected intraperitoneally once a day for 4 weeks. After 4 weeks administration, transmission electron microscope (TEM) observation of morphology of rat myocardial mitochondria was performed. Serum levels of succinate dehydrogenase (SDH), superoxide dismutase (SOD), malondialdehyde (MDA) and cardiac troponin I (cTnI) were detected to evaluate mitochondrial enzymes activities. Light micrograph of rat myocardiocytes in the control group showing normal architecture of myocytes. The numerical density and number of mitochondria in both low-dose and high-dose AA groups were lower than that of the control group. After administration of LC, the rats in the AA + LC group showed an obvious increase in the numerical density and number of mitochondria. TEM showed that both low-dose and high-dose AA could induce myocardial mitochondrial damage in rats in a dose-dependent manner, such as mitochondrial swelling, disruptions of crest and membrane, mitochondrial deficiency. The degree of mitochondrial damage of the AA + LC group was significantly decreased after administration of LC. Our results showed that serum levels of SDH and SOD in the AA + LC and control groups were also higher than those of the low-dose and high-dose AA groups; while the MDA level in the AA + LC and control groups were lower than that of the low-dose and high-dose AA groups. The low-dose AA, high-dose AA and AA + LC groups exhibited a higher level of serum cTnI than that of the control group. However, there was no significant difference in serum cTnI level among the low-dose AA, high-dose AA and AA + LC groups. Our findings indicate that AA may lead to myocardial mitochondrial damage and the induction of enzyme activity in rats, while administration of LC could alleviate AA-related damage of rat myocardial mitochondria.


Assuntos
Acetaldeído/toxicidade , Carnitina/farmacologia , Mitocôndrias Cardíacas/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Animais , Masculino , Malondialdeído/sangue , Mitocôndrias Cardíacas/enzimologia , Mitocôndrias Cardíacas/ultraestrutura , Ratos , Ratos Wistar , Succinato Desidrogenase/sangue , Superóxido Dismutase/sangue
9.
Obes Surg ; 24(11): 1862-9, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24831461

RESUMO

OBJECTIVE: This meta-analysis was conducted to determine the effect of percent excess weight loss (%EWL) after bariatric surgery on diabetes remission. METHODS: The Cochrane Library, PubMed, MEDLINE, Embase, and CINAHL were searched. All reports on %EWL involving humans and published in English between 1 January 1992 and 1 September 2013 were included in the analysis. RESULTS: Eight studies involving 1,247 patients who underwent bariatric surgery were selected. %EWL was positively associated with remission rate (WMD = 11.15, 95 % CI: 6.73-15.56, p < 0.01) in the Caucasian population. CONCLUSIONS: Patients with extensive weight loss were more likely to achieve T2DM remission after bariatric surgery. Further randomized controlled trials (RCTs) with uniform remission criterion should be performed to provide more reliable evidence.


Assuntos
Diabetes Mellitus Tipo 2/complicações , Obesidade Mórbida/cirurgia , Perda de Peso , Cirurgia Bariátrica/métodos , Diabetes Mellitus Tipo 2/sangue , Humanos , Obesidade Mórbida/complicações , Período Pós-Operatório
10.
Cardiovasc Drugs Ther ; 27(3): 199-210, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23371796

RESUMO

PURPOSE: We assessed whether tetramethylpyrazine (TMP), an active ingredient of Ligusticum wallichii Franchat, attenuates atherosclerosis (AS) development in rabbits and protects endothelial cells injured by ox-LDL. METHODS: In vivo, rabbits subjected to atherosclerosis were treated with TMP (75 and 150 mg/kg) by oral gavage for 12 weeks. In vitro, rat aortic endothelial cells (RAECs) were stimulated by ox-LDL. RESULTS: TMP treatment with 75 and 150 mg/kg significantly reduced the relative atherosclerosis area ratio in the aorta (0.41 ± 0.042, 0.27 ± 0.047 vs. 0.66 ± 0.058 in AS), the ratio of intimal/medial thickness (0.54 ± 0.09, 0.39 ± 0.07 vs. 1.1 ± 0.3 in AS) and the number of monocytes in intimal (10.1 ± 2.8, 8.2 ± 2.0 vs. 14.1 ± 4.9 counts/mm(2) in AS). TMP also decreased levels of TC (15 ± 4.2 to 6.1 ± 1.2 mmol/L), TG (1.8 ± 0.3 to 1.08 ± 0.24 mmol/L), LDL-C (20.1 ± 4.3 to 10.2 ± 1.6 mmol/L) and increased HDL-C levels (0.40 ± 0.08 to 0.85 ± 0.17 mmol/L) in atherosclerosis rabbit plasma. TMP decreased the MCP-1 (187.3 ± 38.4 to 86.1 ± 17.2 pg/ml) and ICAM-1 (350.6 ± 43.7 to 260.6 ± 46.1 pg/ml) levels in plasma and inhibited LOX-1 expression in the rabbit aortas. Moreover, our in vitro study revealed that TMP suppressed monocyte adhesion to RAECs, inhibited RAEC migration, and down-regulated MCP-1 and ICAM-1 expression in ox-LDL-injured RAECs. Likewise, TMP inhibited LOX-1 and 5-LOX expression, and prevented nuclear accumulation of RelA/p65 and IκB degradation in ox-LDL-injured RAECs. Furthermore, TMP suppressed ox-LDL-induced activations of p-ERK, p-p38, and p-JNK MAPK. CONCLUSION: TMP produces a tangible protection in atherosclerosis and endothelial cells. TMP might be a potential protective agent for atherosclerosis.


Assuntos
Aterosclerose/prevenção & controle , Células Endoteliais/efeitos dos fármacos , Lipoproteínas LDL/efeitos adversos , Placa Aterosclerótica/prevenção & controle , Pirazinas/uso terapêutico , Animais , Aorta Abdominal/efeitos dos fármacos , Aorta Abdominal/patologia , Aterosclerose/sangue , Aterosclerose/patologia , Adesão Celular/efeitos dos fármacos , Técnicas de Cultura de Células , Movimento Celular/efeitos dos fármacos , Células Cultivadas , Colesterol/sangue , Colesterol na Dieta/administração & dosagem , Modelos Animais de Doenças , Imuno-Histoquímica , Ligusticum/química , Masculino , Placa Aterosclerótica/sangue , Placa Aterosclerótica/patologia , Pirazinas/administração & dosagem , Pirazinas/isolamento & purificação , Coelhos , Ratos , Ratos Sprague-Dawley , Triglicerídeos/sangue
11.
PLoS One ; 7(11): e49701, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23166749

RESUMO

Paeoniflorin (PF), the principal component of Paeoniae Radix prescribed in traditional Chinese medicine, has been reported to exhibit many pharmacological effects including protection against ischemic injury. However, the mechanisms underlying the protective effects of PF on cerebral ischemia are still under investigation. The present study showed that PF treatment for 14 days could significantly inhibit transient middle cerebral artery occlusion (MCAO)-induced over-activation of astrocytes and microglia, and prevented up-regulations of pro-inflamamtory mediators (TNFα, IL-1ß, iNOS, COX(2) and 5-LOX) in plasma and brain. Further study demonstrated that chronic treatment with PF suppressed the activations of JNK and p38 MAPK, but enhanced ERK activation. And PF could reverse ischemia-induced activation of NF-κB signaling pathway. Moreover, our in vitro study revealed that PF treatment protected against TNFα-induced cell apoptosis and neuronal loss. Taken together, the present study demonstrates that PF produces a delayed protection in the ischemia-injured rats via inhibiting MAPKs/NF-κB mediated peripheral and cerebral inflammatory response. Our study reveals that PF might be a potential neuroprotective agent for stroke.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Benzoatos/farmacologia , Isquemia Encefálica/metabolismo , Hidrocarbonetos Aromáticos com Pontes/farmacologia , Glucosídeos/farmacologia , Inflamação/metabolismo , Proteínas Quinases Ativadas por Mitógeno/metabolismo , NF-kappa B/metabolismo , Animais , Anti-Inflamatórios não Esteroides/administração & dosagem , Astrócitos/efeitos dos fármacos , Astrócitos/metabolismo , Benzoatos/administração & dosagem , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Encéfalo/patologia , Isquemia Encefálica/tratamento farmacológico , Hidrocarbonetos Aromáticos com Pontes/administração & dosagem , Infarto Cerebral/tratamento farmacológico , Infarto Cerebral/metabolismo , Infarto Cerebral/patologia , Ciclo-Oxigenase 2/metabolismo , Citocromos c/genética , Citocromos c/metabolismo , Modelos Animais de Doenças , Regulação da Expressão Gênica/efeitos dos fármacos , Glucosídeos/administração & dosagem , Hipocampo/efeitos dos fármacos , Inflamação/tratamento farmacológico , Interleucina-1beta/sangue , Interleucina-1beta/genética , Lipoxigenase/metabolismo , Masculino , Microglia/efeitos dos fármacos , Microglia/metabolismo , Proteínas Quinases Ativadas por Mitógeno/antagonistas & inibidores , Monoterpenos , NF-kappa B/antagonistas & inibidores , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos , Transdução de Sinais/efeitos dos fármacos , Fator de Necrose Tumoral alfa/sangue , Fator de Necrose Tumoral alfa/genética , Proteína X Associada a bcl-2/genética , Proteína X Associada a bcl-2/metabolismo
13.
Phys Rev Lett ; 106(24): 248302, 2011 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-21770606

RESUMO

The atomic scale lithiation mechanism of individual SnO2 nanowires in a flooding geometry was revealed by in situ transmission electron microscopy. The lithiation was initiated by the formation of multiple stripes with a width of a few nanometers parallel to the (020) plane traversing the entire wires, serving as multiple reaction fronts for later stages of lithiation. Inside the stripes, we identified a high density of dislocations and enlarged interplanar spacing, which provided an effective path for lithium ion transport. The density of the stripes increased with further lithiation, and eventually they merged with one another, causing a large elongation, volume expansion, and the crystalline-to-amorphous phase transformation. This lithiation mechanism characterized by multiple stripes and multiple reaction fronts was unexpected and differed completely from the expected core-shell lithiation mechanism.

14.
J Biomed Biotechnol ; 2011: 793198, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21660143

RESUMO

The tetraploid plants of Catharanthus roseus (L.) G. Don was obtained by colchicine induction from seeds explants, and the ploidy of the plants was identified by flow cytometry. The optimal treatment is 0.2% colchicine solution treated for 24 hours, and the induction rate reaches up to 30%. Comparing with morphological characteristics and growth habits between tetraploids and the control, we found that tetraploids of C. roseus had larger stoma and more branches and leaves. HPLC analysis showed tetraploidization could increase the contents of terpenoid indole alkaloids in C. roseus. Thus, tetraploidization could be used to produce higher alkaloids lines for commercial use. QRT-PCR results showed that the expression of enzymes involved in terpenoid indole alkaloids biosynthesis pathway had increased in the tetraploid plants. To our knowledge, this was the first paper to explore the secondary metabolism in autotetraploid C. roseus induced by colchicine.


Assuntos
Catharanthus/efeitos dos fármacos , Catharanthus/genética , Colchicina/farmacologia , Citometria de Fluxo/métodos , Alcaloides de Triptamina e Secologanina/isolamento & purificação , Alcaloides de Triptamina e Secologanina/metabolismo , Sementes/genética , Análise de Variância , Catharanthus/metabolismo , Expressão Gênica , Fenótipo , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/metabolismo , Folhas de Planta/genética , Folhas de Planta/metabolismo , Sementes/metabolismo , Tetraploidia
15.
J Bacteriol ; 193(14): 3670-1, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21602328

RESUMO

Enterobacter mori is a plant-pathogenic enterobacterium responsible for the bacterial wilt of Morus alba L. Here we present the draft genome sequence of the type strain, LMG 25706. To the best of our knowledge, this is the first genome sequence of a plant-pathogenic bacterium in the genus Enterobacter.


Assuntos
Enterobacter/genética , Genoma Bacteriano , Morus/microbiologia , Doenças das Plantas/microbiologia , Sequência de Bases , Enterobacter/classificação , Enterobacter/isolamento & purificação , Dados de Sequência Molecular , Análise de Sequência de DNA
17.
J Nanosci Nanotechnol ; 10(3): 2028-31, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20355621

RESUMO

NaYF4:Yb3+,Tm3+ upconversion nanocrystals were synthesized with oleic acid as a capping ligand. The as-prepared samples were redispersed in cyclohexane, forming transparent and stable solutions which perform bright blue upconversion. Inducing TiO2 as the outer layer of the fluoride cores, we investigated the effect of TiO2 on the upconversion process. The transmission electron microscopy (TEM) analysis indicated that titania successfully formed a coating layer on the surface of NaYF4:Yb3+, Tm3+ nanoparticles, and the average diameters of the NaYF4:Yb3+,Tm3+ nanocrystals and the NaYF4:Yb3+, Tm3+/TiO2 nanoparticles are about 14 and 40 nm, respectively. Under the excitation of a 980 nm laser, NaYF4:Yb3+, Tm3+ nanocrystals and TiO2-coated NaYF4:Yb3+, Tm3 core/shell structure sample exhibited distinct upconversion luminescent properties.

18.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 34(5): 427-31, 2005 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-16216054

RESUMO

OBJECTIVE: To investigate the correlation of multiple primary lung cancer with bronchial epithelial dysplasia and atypical adenomatous hyperplasia of bronchiolo-alveolar epithelium. METHODS: Careful pathological examinations were performed on 114 surgical specimens of primary lung carcinoma. The correlation of multiple primary lung cancer with bronchial epithelial dysplasia and atypical adenomatous hyperplasia of bronchiolo-alveolar epithelium was analyzed. RESULTS: Of 114 cases of primary lung cancer,13 cases of multiple primary lung cancer (11.4 %) was identifiedìwhich consisted of 6 cases containing two primary bronchogenic carcinoma and 7 containing one bronchogenic carcinoma and one bronchiolo-alveolar carcinoma. The rate of multiple primary lung cancers was significantly higher in individuals with high grade bronchial epithelial dysplasia than in those with low grade dysplasia (r=0.238, P<0.05). CONCLUSION: Bronchial and alveolar epithelial cells may develop malignancy synchronously or metachronously. The probability of developing multiple primary lung cancer will increase in the lungs with extensive and severe bronchial epithelial dysplasia.


Assuntos
Brônquios/patologia , Carcinoma de Células Escamosas/patologia , Neoplasias Pulmonares/patologia , Neoplasias Primárias Múltiplas/patologia , Alvéolos Pulmonares/patologia , Adenocarcinoma/patologia , Adulto , Idoso , Carcinoma de Células Pequenas/patologia , Proliferação de Células , Feminino , Humanos , Hiperplasia , Masculino , Pessoa de Meia-Idade , Lesões Pré-Cancerosas/patologia , Mucosa Respiratória/patologia
19.
World J Gastroenterol ; 11(16): 2467-71, 2005 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-15832419

RESUMO

AIM: To evaluate the significance of extended radical operation and its indications. METHODS: Between January 1995 and December 1998, 56 inpatients with pancreatic head cancer received operation. Among them 35 patients (group 1) experienced the Whipple operation, and 21 patients (group 2) received the extended radical operation. The 1-, 2-, 3-year cumulative survival rates were used to evaluate the efficacy of the two operative procedures. Clinical stage (CS) was assessed retrospectively with the help of CT. The indications for extended radical operation were discussed. RESULTS: There was no difference in hospital mortality and morbidity rates. Whereas the 1-, 2-, 3-year cumulative survival rates were 84.8%, 62.8%, 39.9% in the extended radical operation group, and were 70.8%, 47.6%, 17.2% in the Whipple operation group, there was a significant difference between the two groups (P<0.001, P<0.001, P<0.001, respectively). Most of the deaths within 3 years after operation were due to recurrence in the two groups. However, the 1-, 2-, 3-year cumulative rates of death due to local recurrence were decreased from 37.4% in patients that received the Whipple procedure to 23.8% in those who received by extended radical operation. Patients who survived for more than 3 years were only noted in those with CS1 in the Whipple procedure group and were founded in cases with CS1, CS2 and part of CS3 in the extended radical operation group. CONCLUSION: The extended radical operation appears to benefit patients with pancreatic head carcinoma which was indicated in CS1, CS2 and part of CS3 without severe invasion.


Assuntos
Procedimentos Cirúrgicos do Sistema Digestório/mortalidade , Procedimentos Cirúrgicos do Sistema Digestório/métodos , Neoplasias Pancreáticas/mortalidade , Neoplasias Pancreáticas/cirurgia , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Neoplasias Pancreáticas/patologia , Estudos Retrospectivos , Taxa de Sobrevida , Resultado do Tratamento
20.
Zhonghua Zhong Liu Za Zhi ; 26(3): 173-6, 2004 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-15196442

RESUMO

OBJECTIVE: To evaluate the significance of extended radical resection in the treatment of pancreatic head cancer and its indication. METHODS: Between Jan. 1995 and Dec. 1998, 56 patients with pancreatic head cancer were retrospectively reviewed, among whom 35 were treated by the Whipple operation and 21 received the extended radical resection during the same interval. RESULTS: There was no significant difference between the mortality and morbidity rate of complication, though with more patients having higher clinical stages in the extended radical resection group. The 1-, 2- and 3-year survival rates were 84.8%, 62.8%, 39.9% in the extended radical resection group and 70.8%, 47.6%, 17.2% in the Whipple operation group with significant difference between the two groups. The total mortality rate was 51.4% in Whipple group and 42.9% in extended radical resection group with significant difference between the two. The 3-year cumulative rate of death from local recurrence decreased from 37.4% in the Whipple group to 23.8% in the extended radical operation group. Patients who survived for more than 3 years were only those in clinical stage (SC)1 in the Whipple group whereas they were found both in patients who had had CS1, CS2 lesions and also in some who had CS3 lesions in the extended radical resection group. CONCLUSION: The extended radical operation does benefit patients with pancreatic head carcinoma in CS1, CS2 and in a part of CS3 without too extensive exrtra-pancreatic invasion.


Assuntos
Neoplasias Pancreáticas/cirurgia , Idoso , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Neoplasias Pancreáticas/mortalidade , Neoplasias Pancreáticas/patologia , Estudos Retrospectivos , Taxa de Sobrevida
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