Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 10 de 10
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
BMC Genomics ; 21(1): 187, 2020 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-32111155

RESUMO

BACKGROUND: Aohan fine wool sheep (AFWS) is a historically bred fine wool sheep, cultivated in China. The wool has excellent quality and good textile performance. Investigating the molecular mechanisms that regulate wool growth is important to improve wool quality and yield. Circular RNAs (circRNAs) are widely expressed non-coding RNAs that can act as competitive endogenous RNAs (ceRNAs) to bind to miRNAs. Although circRNAs have been studied in many fields, research on their activity in sheep wool follicles is limited. To understand the regulation of circRNAs in the growth of fine wool in sheep, we used RNA-Seq to identify circRNAs in sheep shoulder skin samples at three developmental stages: embryonic day 90 (E90d), embryonic day 120 (E120d), and at birth (Birth). RESULTS: We identified 8753 circRNAs and found that 918 were differentially-expressed. We then analyzed the classification and characteristic of the circRNAs in sheep shoulder skin. Using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), we identified the source genes of circRNAs, which were mainly enriched in cellular component organization, regulation of primary metabolic processes, tight junctions, and the cGMP-PKG and AMPK signaling pathways. In addition, we predicted interactions between 17 circRNAs and eight miRNAs, using miRanda software. Based on the significant pathways, we speculate that circ_0005720, circ_0001754, circ_0008036, circ_0004032, circ_0005174, circ_0005519, and circ_0007826 might play an important role in regulating wool follicle growth in AFWS. Seven circRNAs were randomly selected to validate the RNA-Seq results, using qRT-PCR. CONCLUSION: Our results provide more information about circRNAs regulation of wool follicle development in AFWS, and establish a solid foundation for future research.

2.
Food Sci Nutr ; 7(4): 1295-1301, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31024702

RESUMO

Obesity has become one of the most important health problems worldwide requiring urgent need for efficient control. Pleurotus citrinopileatus (P. citrinopileatus)-a type of edible mushroom with abundant bioactive molecules-is a promising source for achieving this goal. In the present study, we evaluated the anti-obesity and hypolipidemic effect of P. citrinopileatus water extract (PWE) using a series of biochemical assays in randomized high-fat diet-induced obese (DIO) C57BL/6J mice, which were gavaged daily with low or high levels of PWE (400 or 800 mg/kg of body weight, respectively) in addition to high-fat diet for 12 weeks. Results showed that PWE significantly reduced the weight gain, fat accumulation, and food intake of DIO mice within 12 weeks. PWE also decreased the serum triglycerides, cholesterol and low-density lipoprotein, aspartate transaminase, nonesterified fatty acid, and creatinine, but increased high-density lipoprotein. Additionally, PWE improved the glucose tolerance of mice fed with high fat. From above, we conclude that PWE has great potential as functional foods for management of obesity and/or associated metabolic disorders.

3.
Int J Med Mushrooms ; 19(11): 1001-1008, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29345562

RESUMO

Pleurotus citrinopileatus is an edible medicinal mushroom rich in biomolecules and thus has a high potential for use in formulating pharmaceutical and nutraceutical products. To test its effect on body weight and glucose control, we generated diet-induced obese (DIO) C57BL/6J male mice by feeding the mice a high-fat diet (60% fat) for 8 weeks and treating them with an ethanolic P. citrinopileatus extract (PCE) at either 200 or 500 mg/kg body weight for 12 additional weeks. The results showed that PCE significantly inhibited high-fat diet-induced weight gain, fat accumulation, and glucose intolerance in the DIO mice. Moreover, the PCE had a beneficial effect on liver and kidney function. On the basis of these results, we conclude that PCE is effective in the treatment of metabolic syndrome and thus could be a good candidate for use in future pharmaceutical or nutraceutical applications.


Assuntos
Misturas Complexas/administração & dosagem , Misturas Complexas/isolamento & purificação , Síndrome Metabólica/tratamento farmacológico , Pleurotus/química , Tecido Adiposo/patologia , Animais , Peso Corporal , Intolerância à Glucose/prevenção & controle , Camundongos Endogâmicos C57BL , Camundongos Obesos , Obesidade/prevenção & controle , Resultado do Tratamento
4.
Sci Rep ; 6: 26845, 2016 05 27.
Artigo em Inglês | MEDLINE | ID: mdl-27228948

RESUMO

Most terrestrial ecosystems are nitrogen (N)-limited. The elucidation of the multivariate relationships among environmental drivers, leaf morphological traits, and foliar N of dominant species which are critical to the functioning of forests remains a critical challenge for ecologists. We sampled leaves of Quercus wutaishanica across a broad natural gradient in the Loess Plateau, China, and employed structural equation modelling to evaluate the causal pathways and the relative importance of drivers of the foliar N per unit area (Narea) and per unit mass (Nmass). We found that (1) Nmass and Narea were primarily affected by leaf morphological traits instead of environmental variables and that leaf morphological traits accounted for most of their variations; (2) the total soil potassium and phosphorus and mean annual precipitation had different effects on Nmass and Narea via different pathways and path coefficients, whereas the mean annual temperature and total soil N had non-significant effects on Nmass and Narea. Our results demonstrated that variations in Nmass and Narea within Quercus wutaishanica were strongly linked to their leaf morphological traits and that the leaf N was also influenced by mean annual precipitation and soil phosphorus and potassium instead of soil N in the Loess Plateau, China.

5.
Int J Med Mushrooms ; 17(7): 671-80, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26559701

RESUMO

Extra- (EPS) and intracellular polysaccharides (IPS) from Pholiota nameko SW-01 were extracted, and in vitro and in vivo antioxidant effects were processed. Briefly, the in vitro antioxidant results indicated that the inhibition effects of IPS at a dose of 500 mg/L on superoxide anions, hydroxyl radicals, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 63.24 ± 1.12%, 53.11 ± 2.92%, and 59.33 ± 9.37%, which were 7.70%, 14.84%, and 11.40% higher than those of EPS, and 9.79%, 20.32%, and 4.59% higher than those of butylated hydroxytoluene (BHT), respectively. The reducing power of IPS reached 0.593 ± 0.21 (absorbance at 700 nm), which was 11.30% higher than that of EPS and 15.51% higher than that of BHT. In vivo antioxidant results proved that both IPS and EPS significantly upregulated the enzyme activities of superoxide dismutase (SOD), GSH peroxide (GSH-Px), catalase (CAT), and alanine transaminase (ALT). In addition, glycosidic bond types of EPS and IPS were primarily characterized. The findings indicate the potential antioxidant effects of polysaccharide extract from Ph. Nameko. Thus, this species has potential applicability as an antioxidant agent and should be studied further.


Assuntos
Agaricales/química , Antioxidantes/farmacologia , Polissacarídeos/farmacologia , Animais , Antioxidantes/isolamento & purificação , Fermentação , Camundongos , Polissacarídeos/isolamento & purificação
6.
Zhonghua Zhong Liu Za Zhi ; 36(12): 897-902, 2014 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-25623762

RESUMO

OBJECTIVE: To investigate the reversal effect of targeted modulation of bcl-2 expression by miR-15a and miR-16 on drug resistance of human colon cancer cells. METHODS: Mimics or inhibitors of miR-15a and miR-16 were transfected into HCT8 or HCT8/VCR cells with the help of Lipofectamine 2000. The expressions of miR-15a and miR-16 mRNA were detected by RT-qPCR. The levels of bcl-2 and P-gp proteins were measured by Western blot. The inhibitory effects of VCR on growth of HCT8 and HCT8/VCR cells were detected by CCK8. RESULTS: After transfection of the mimics, the expression of miR-15a in the blank control group, negative control group and miR-15a mimic group was 1.00, 0.87 ± 0.24, and 223.44 ± 59.07, respectively, and miR-15a was increased significantly (P < 0.001). The expression of miR-16 in the blank control group, negative control group and miR-16 mimic group was 1.00, 0.66 ± 0.19, and 107.32 ± 22.58, respectively, and miR-16 expression was increased significantly (P < 0.001). The Western blot assay showed that the relative expressions of bcl-2 protein in the blank control group, negative control group, miR-15a mimic group and miR-16 mimic group were 1.00, 0.97 ± 0.02, 0.51 ± 0.06, and 0.65 ± 0.03, respectively, and the expression of bcl-2 protein was decreased significantly (P < 0.05), however, the expressions of P-gp protein showed no significant difference. The CCK8 test showed that at 1, 5, 25 and 125 µg/ml concentration of VCR, the survival rates of HCT8/VCR cells were basically the same in the blank control group, negative control group, miR-15a mimic group and miR-16 mimic group, but the survival rate of HCT8/VCR cells was significantly decreased after transfection of mimics (P < 0.05). After transfection of the inhibitors, the expressions of both miR-15a and miR-16 were decreased significantly (P < 0.001). The Western blot showed that the expression of bcl-2 protein was increased (P < 0.05), while the expression of P-gp protein showed no significant difference. The CCK8 test showed that the survival rate of HCT8 cells which were transfected with inhibitors was significantly higher than that of the blank control group (P < 0.05). CONCLUSIONS: miR-15a and miR-16 may reverse the drug resistance in human colon cancer cells. A possible mechanism is regulating the expression of bcl-2.


Assuntos
MicroRNAs/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Membro 1 da Subfamília B de Cassetes de Ligação de ATP , Apoptose , Proteínas Reguladoras de Apoptose/metabolismo , Linhagem Celular Tumoral , Neoplasias do Colo , Resistência a Medicamentos , Humanos , Proteínas Proto-Oncogênicas c-bcl-2/genética , RNA Mensageiro , Transfecção
7.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 31(6): 1394-9, 2014 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-25868266

RESUMO

Mammography imaging is one of the most demanding imaging modalities from the point of view of the bal- ance between image quality (the visibility of small size and/or low contrast structures) and dose (screening of many asymptomatic people). Therefore, since the introduction of the first dedicated mammographic units, many efforts have been directed to seek the best possible image quality while minimizing patient dose. The performance of auto- matic exposure control (AEC) is the manifestation of this demand. The theory of AEC includes exposure detection and optimization and also involves some accomplished methodology. This review presents the development and present situa- tion of spectrum optimization, detector evolution, and the way how to accomplish and evaluate AEC methods.


Assuntos
Mamografia/métodos , Humanos , Imagens de Fantasmas , Doses de Radiação , Interpretação de Imagem Radiográfica Assistida por Computador
8.
J Biosci Bioeng ; 111(1): 50-4, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20801714

RESUMO

Response surface methodology (RSM) was used to optimize the extraction parameters for Pholiota adiposa SX-02 intracellular polysaccharide (IPS) produced during submerged culture. The optimum conditions of IPS extraction were predicted to be, ultrasonic power at 564.93 W, precipitation time 30.34 h and pH 8.28, and IPS yield was estimated at 19.75%. The actual value of IPS under these conditions was 20.51%. The in vitro antioxidant results showed that the inhibition effects of IPS at a dosage of 250 mg/l on superoxide anion, hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were 74.66 ± 5.31%, 69.20 ± 5.13%, and 75.20 ± 6.73%, respectively, which were 27.51 ± 2.23%, 16.58 ± 1.33%, and 9.46 ± 0.72% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of IPS was 0.32 ± 0.02 (absorbance at 700 nm), 39.13 ± 3.47% higher than that of BHT. The results provide a reference for large-scale extraction of IPS by P. adiposa SX-02 in industrial fermentation and the IPS can be used as a potential antioxidant which enhances adaptive immune responses.


Assuntos
Depuradores de Radicais Livres/isolamento & purificação , Pholiota/metabolismo , Polissacarídeos/isolamento & purificação , Compostos de Bifenilo/metabolismo , Meios de Cultura , Depuradores de Radicais Livres/farmacologia , Concentração de Íons de Hidrogênio , Radical Hidroxila/metabolismo , Picratos/metabolismo , Polissacarídeos/farmacologia
9.
Int J Biol Macromol ; 47(2): 116-9, 2010 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-20580645

RESUMO

The extraction conditions of intracellular polysaccharide (IPS) from Pleurotus sp. mycelium in submerged culture were investigated. Four parameters affecting the IPS extraction, ultrasonic treatment time, extraction temperature, extraction time and ethanol concentration, were determined by single factor tests and then optimized by orthogonal experiments. Under the optimized conditions, the extraction rates of IPS of Pleurotus nebrodensis SJ-02, Pleurotus eryngii SI-01 and Pleurotus corncopiae SS-01 were 7.1+/-0.4%, 7.5+/-0.3%, and 8.2+/-0.5%, respectively. The in vitro hydroxyl radical inhibition percentages of IPS of three mushrooms were 32.2+/-2.8%, 16.1+/-1.5%, and 38.7+/-3.1%, respectively. The scavenging effects of IPS on superoxide anion radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were 19.1+/-1.5%, 16.3+/-1.3%, 20.3+/-1.8%, 17.9+/-1.6%, 16.8+/-1.4%, and 20.5+/-1.7%, respectively. The results provide a reference for large-scale production of IPS by Pleurotus sp. in industrial fermentation.


Assuntos
Depuradores de Radicais Livres/isolamento & purificação , Depuradores de Radicais Livres/farmacologia , Espaço Intracelular/química , Micélio/citologia , Pleurotus/citologia , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Compostos de Bifenilo/metabolismo , Depuradores de Radicais Livres/metabolismo , Radical Hidroxila/metabolismo , Picratos/metabolismo , Polissacarídeos/metabolismo , Superóxidos/metabolismo
10.
Bioresour Technol ; 101(12): 4564-9, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20153962

RESUMO

Response surface methodology was used to optimize extraction parameters for Morchella esculenta SO-01 exopolysaccharide (EPS) produced during submerged culture. The optimum conditions for EPS extraction were predicted to be, concentration at 84.07 degrees C, precipitation for 22.19 h and pH 8.44, and EPS production was estimated at 5.45 g/L. The actual yield of EPS under these conditions was 5.32 g/L. The antioxidant capacity of the EPS was measured in vivo after filling the stomach with different doses of EPS and results showed that the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of blood, spleen, liver, heart, and kidney were increased by 125%, 46.11%, 23.33%, 12.19%, 41.29%, and 63.24%, 63.12%, 166.54%, 98.01%, 57.68%, respectively, and that malonaldehyde (MDA) of blood, spleen, liver, heart, and kidney were decreased by 21.80%, 67.84%, 28.48%, 56.15%, 41.62%. The results provide a reference for large-scale extraction of EPS by M. esculenta SO-01 in industrial fermentation.


Assuntos
Agaricales/química , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Biotecnologia/métodos , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Análise de Variância , Animais , Glutationa Peroxidase/metabolismo , Malondialdeído/metabolismo , Camundongos , Especificidade de Órgãos/efeitos dos fármacos , Superóxido Dismutase/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA