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1.
Front Neurol ; 12: 735201, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34630306

RESUMO

Objectives: To explore the trend of sudden unexpected death in epilepsy (SUDEP) incidence rate over time in rural west China. Methods: We scanned probable SUDEP patients from the epilepsy program between 2010 and 2019 in rural West China and performed a verbal autopsy for each eligible patient. We calculated the crude and sex-adjusted incidence rate of SUDEP per person-year over a calendar year and the year of follow-up. We calculated the incidence rate ratio with the Poisson model in STATA 12.0 and calculated the annual percentage change (APC) and average annual percentage change in Joinpoint Trend Analysis Software 4.8.0.1 to analyze the trend of SUDEP incidence rate. Results: In 2010-2019, 44 probable SUDEPs were identified from 10,128 patients with a total person-year of 31,347. The crude and sex-adjusted incidence rates of SUDEP were 1.40 and 1.45%0. Twenty-five (56.8%) of the 44 probable SUDEPs had no generalized tonic-clonic seizure 3 months before their death. The incidence of probable SUDEP decreased significantly in the calendar year [APC = -11.7, 95% confidence interval (CI): -21.7 to -0.3] and in time of follow-up (average annual percentage change = -21.2, 95% CI: -34.3 to -5.4). Comparing the first 5 years in follow-up with the subsequent 3 years, the incidence rate of SUDEP decreased significantly (estimated incidence rate ratio = 0.4, 95% CI: 0.2 to 0.8). Significance: SUDEP happened to 1.4 cases per thousand patient-years in convulsive epilepsy in rural west China between 2010 and 2019. The incidence rate of SUDEP presented a downward trend over the time of follow-up.

2.
Epilepsia ; 62(10): 2463-2473, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34342885

RESUMO

OBJECTIVE: To characterize the features of thalamocortical functional connectivity during seizure recurrence at the time of antiseizure medication (ASM) withdrawal. METHODS: Patients with chronic epilepsy who attempted to discontinue medications were prospectively registered and followed up; 19 patients remained seizure-free (SF-group), 18 patients had seizure relapses (SR-group) after ASM withdrawal, and 28 healthy controls were recruited. Resting-state functional magnetic resonance imaging was performed before ASM withdrawal. Thalamus subdivisions were set as seeds to calculate voxelwise functional connectivity. Partial correlation analysis between functional connectivity and clinical variables was performed. A support vector machine was used to assess the predictive ability of the specific functional connectivity for seizure relapse. RESULTS: The within-group comparison indicated that the SR-group had more extensive functional connectivity than the SF-group; the left inferior pulvinar, left medial pulvinar, and right anterior pulvinar showed a significantly stronger functional connection with the precuneus in the SR-group than in the SF-group (Gaussian random field correction, voxel-level p < .001 and cluster-level p < .05). In the SR-group, a positive correlation was found between the left inferior pulvinar-precuneus connectivity and the active period (r = .46, p = .05), seizure-free period (r = .67, p = .002), and disease duration (r = .53, p = .02), and between the left medial pulvinar-precuneus connectivity and the seizure-free period (r = .58, p = .01). The combination of these thalamocortical connections showed a high predictive ability, with an area under the curve of .92 and accuracy of .90 (p = .01). SIGNIFICANCE: This study determined distinct features of thalamocortical functional connectivity at the time of ASM withdrawal in patients with and without seizure relapse, showing a potential for predicting seizure outcomes following ASM withdrawal.

3.
Dalton Trans ; 50(35): 12188-12196, 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34382986

RESUMO

Because of direct π-π interactions and excessive energy resonance transfer, it is very challenging to prepare carbon dots (CDs) with a high fluorescence quantum yield (QY) in the solid state. In this study, novel CDs which gave solid-state fluorescence (SSF) with high brightness were successfully prepared via a simple microwave-assisted method. The prepared ScCDs can emit strong blue fluorescence in the solid state, and the absolute QY of this ScCDs powder reaches 51.7%. Such a high QY means that the ScCDs powder could be successfully applied in rapid latent fingerprint (LFP) detection. The LFP detection performance of this ScCDs powder was studied in detail, and the results show that the LFPs developed using the ScCDs powder can be visualized with high definition and contrast under different conditions. This research not only developed a new type of SSF-emitting CDs, but it also proved that the developed CDs have great potential for applications in LFP detection, and this research may also provide inspiration and ideas for the design of new SSF-emitting CDs.

4.
Epilepsy Res ; 176: 106740, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34419771

RESUMO

OBJECTIVES: A considerable proportion of epilepsy patients who achieved long-term seizure freedom with standardized treatment of antiseizure medication will attempt to withdraw medications. Epilepsy is currently considered as a network disease, however, the characteristics of brain function and neural network before and after medication withdrawal remain to be discovered. METHODS: Resting-state functional magnetic resonance imaging was obtained for 32 healthy controls, 32 seizure-free patients initiating medication tapering (PG1 group), and 16 seizure-free patients that had completely discontinued medications (PG2 group). Amplitude of low-frequency fluctuation and regional homogeneity were calculated to measure local functional activity. Global and nodal metrics of small-world network were calculated based on Graph theory. One-way analysis of variance was applied to analyze intergroup difference, withpost hoc analysis being conducted for each pair of groups. RESULTS: Sex, age at scanning and other clinical variables showed no significant difference between groups. As compared to control, the amplitude of low-frequency fluctuation, regional homogeneity or nodal metrics of neural network in some brain areas were abnormal in the PG1 or PG2 group; when compared between patient groups, significant between-group differences were also found in the amplitude of low-frequency fluctuation, regional homogeneity or nodal metrics. But, the global metrics of neural network showed no differences among groups. CONCLUSIONS: The global metrics of patients with long-term seizure freedom were normal either before or after antiseizure medication withdrawal, while the local functional activity and nodal metrics in some brain areas were abnormal and differed between before and after antiseizure medication withdrawal.

5.
Seizure ; 91: 491-498, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34358846

RESUMO

Purpose To evaluate the incidence rate and risk factors for status epilepticus (SE) after stroke (PSSE), including ischaemic stroke (IS), intracerebral hemorrhage (ICH), and subarachnoid hemorrhage (SAH). Methods A meta-analysis was performed using relevant research from databases such as PubMed, Embase, Cochrane Online Library, and Clinicaltrials.gov. The quality of the studies was evaluated by using the quality evaluation criteria of the Agency for Healthcare Research and Quality (AHRQ). All data were pooled by STATA 12.0 software for meta-analysis. Results The review considered 1650 articles, and 17 articles with 2821 instances of SE among 1088087 instances of stroke were included. The incidence rate of SE after stroke was 6.90 per 1000 total strokes (95% CI: 5.58-8.22). By subgroup analysis of SE, the rates were 33.85‰ (95% CI: 13.77-53.94) for non-convulsive status epilepticus (NCSE) and 2.42‰ (95%CI: 1.66-3.19) for generalized convulsive status epilepticus (GCSE). Age, sex, and presence of atrial fibrillation showed no significant difference between the SE group and the non-SE group after stroke. Hypertension and diabetes are associated with a decreased rate of SE. However, African American race, alcohol abuse, and renal disease are associated with an increased rate of SE. Significance There were approximately 6.9 patients with status epilepticus per 1000 strokes. NCSE is more common after stroke and needs more attention. African American race, alcohol abuse and renal disease may be risk factors for PSSE.


Assuntos
Isquemia Encefálica , Estado Epiléptico , Acidente Vascular Cerebral , Humanos , Incidência , Fatores de Risco , Estado Epiléptico/epidemiologia , Estado Epiléptico/etiologia , Acidente Vascular Cerebral/complicações , Acidente Vascular Cerebral/epidemiologia
6.
J Med Internet Res ; 23(8): e25090, 2021 08 19.
Artigo em Inglês | MEDLINE | ID: mdl-34420931

RESUMO

BACKGROUND: Posttraumatic epilepsy (PTE) is a common sequela after traumatic brain injury (TBI), and identifying high-risk patients with PTE is necessary for their better treatment. Although artificial neural network (ANN) prediction models have been reported and are superior to traditional models, the ANN prediction model for PTE is lacking. OBJECTIVE: We aim to train and validate an ANN model to anticipate the risks of PTE. METHODS: The training cohort was TBI patients registered at West China Hospital. We used a 5-fold cross-validation approach to train and test the ANN model to avoid overfitting; 21 independent variables were used as input neurons in the ANN models, using a back-propagation algorithm to minimize the loss function. Finally, we obtained sensitivity, specificity, and accuracy of each ANN model from the 5 rounds of cross-validation and compared the accuracy with a nomogram prediction model built in our previous work based on the same population. In addition, we evaluated the performance of the model using patients registered at Chengdu Shang Jin Nan Fu Hospital (testing cohort 1) and Sichuan Provincial People's Hospital (testing cohort 2) between January 1, 2013, and March 1, 2015. RESULTS: For the training cohort, we enrolled 1301 TBI patients from January 1, 2011, to December 31, 2017. The prevalence of PTE was 12.8% (166/1301, 95% CI 10.9%-14.6%). Of the TBI patients registered in testing cohort 1, PTE prevalence was 10.5% (44/421, 95% CI 7.5%-13.4%). Of the TBI patients registered in testing cohort 2, PTE prevalence was 6.1% (25/413, 95% CI 3.7%-8.4%). The results of the ANN model show that, the area under the receiver operating characteristic curve in the training cohort was 0.907 (95% CI 0.889-0.924), testing cohort 1 was 0.867 (95% CI 0.842-0.893), and testing cohort 2 was 0.859 (95% CI 0.826-0.890). Second, the average accuracy of the training cohort was 0.557 (95% CI 0.510-0.620), with 0.470 (95% CI 0.414-0.526) in testing cohort 1 and 0.344 (95% CI 0.287-0.401) in testing cohort 2. In addition, sensitivity, specificity, positive predictive values and negative predictors in the training cohort (testing cohort 1 and testing cohort 2) were 0.80 (0.83 and 0.80), 0.86 (0.80 and 0.84), 91% (85% and 78%), and 86% (80% and 83%), respectively. When calibrating this ANN model, Brier scored 0.121 in testing cohort 1 and 0.127 in testing cohort 2. Compared with the nomogram model, the ANN prediction model had a higher accuracy (P=.01). CONCLUSIONS: This study shows that the ANN model can predict the risk of PTE and is superior to the risk estimated based on traditional statistical methods. However, the calibration of the model is a bit poor, and we need to calibrate it on a large sample size set and further improve the model.

7.
Mol Carcinog ; 60(10): 661-670, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34197655

RESUMO

Forkhead box class O (FOXO) transcription factors play a pivotal role in regulating a variety of biological processes, including organismal development, cell signaling, cell metabolism, and tumorigenesis. Therefore, we hypothesize that genetic variants in FOXO pathway genes are associated with breast cancer (BC) risk. To test this hypothesis, we conducted a large meta-analysis using 14 published genome-wide association study (GWAS) data sets in the Discovery, Biology, and Risk of Inherited Variants in Breast Cancer (DRIVE) study. We assessed associations between 5214 (365 genotyped in DRIVE and 4849 imputed) common single-nucleotide polymorphisms (SNPs) in 55 FOXO pathway genes and BC risk. After multiple comparison corrections by the Bayesian false-discovery probability method, we found five SNPs to be significantly associated with BC risk. In stepwise multivariate logistic regression analysis with adjustment for age, principal components, and previously published SNPs in the same data set, three independent SNPs (i.e., FBXO32 rs10093411 A>G, FOXO6 rs61229336 C>T, and FBXO32 rs62521280 C>T) remained to be significantly associated with BC risk (p = 0.0008, 0.0011, and 0.0017, respectively). Additional expression quantitative trait loci analysis revealed that the FBXO32 rs62521280 T allele was associated with decreased messenger RNA (mRNA) expression levels in breast tissue, while the FOXO6 rs61229336 T allele was found to be associated with decreased mRNA expression levels in the whole blood cells. Once replicated by other investigators, these genetic variants may serve as new biomarkers for BC risk.


Assuntos
Neoplasias da Mama/genética , Neoplasias da Mama/metabolismo , Fatores de Transcrição Forkhead/genética , Variação Genética , Proteínas Musculares/genética , Proteínas Ligases SKP Culina F-Box/genética , Transdução de Sinais , Alelos , Feminino , Fatores de Transcrição Forkhead/metabolismo , Expressão Gênica , Predisposição Genética para Doença , Estudo de Associação Genômica Ampla , Genótipo , Humanos , Proteínas Musculares/metabolismo , Polimorfismo de Nucleotídeo Único , Medição de Risco , Proteínas Ligases SKP Culina F-Box/metabolismo
8.
Neuroradiology ; 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34195875

RESUMO

PURPOSE: We aimed to analyze the characteristics of brain function and microstructure linked to epilepsy relapse after drug withdrawal in patients with focal epilepsy. METHODS: Resting-state functional magnetic resonance imaging and high-resolution T1-weighted images were acquired within 1 month prior to drug withdrawal from 15 patients who did not have epilepsy relapse (PER - group) and 16 patients who subsequently had epilepsy relapse (PER + group). Additionally, 23 healthy participants undergoing the same scanning protocol were included as controls. Fractional amplitude of low-frequency fluctuation (fALFF) and gray matter density (GMD) were compared among groups. Subgroup and correlation analyses were also performed. RESULTS: There were no significant differences in fALFF between patient groups, but the PER + group showed lower GMD in the bilateral calcarine, left precuneus, and right superior temporal gyrus than the PER - group (Gaussian random field correction, voxel-level P < 0.001 and cluster-level P < 0.05). Both increased seizure number and polytherapy were associated with lower GMD; also, patients using other antiseizure medications showed lower GMD than those using only levetiracetam (Gaussian random field correction, voxel-level P < 0.001, and cluster-level P < 0.05). The active period and disease duration showed both positive and negative correlations with GMD, while the seizure-free period mainly showed positive correlations with GMD (uncorrected, P < 0.001). CONCLUSION: Gray matter microstructure, but not local functional activity, showed distinct characteristics between patients with and without epilepsy relapse and may serve as a potential biomarker for predicting seizure recurrence upon drug withdrawal.

9.
Nat Commun ; 12(1): 4368, 2021 07 16.
Artigo em Inglês | MEDLINE | ID: mdl-34272383

RESUMO

Bioproduction of renewable chemicals is considered as an urgent solution for fossil energy crisis. However, despite tremendous efforts, it is still challenging to generate microbial strains that can produce target biochemical to high levels. Here, we report an example of biosynthesis of high-value and easy-recoverable derivatives built upon natural microbial pathways, leading to improvement in bioproduction efficiency. By leveraging pathways in solventogenic clostridia for co-producing acyl-CoAs, acids and alcohols as precursors, through rational screening for host strains and enzymes, systematic metabolic engineering-including elimination of putative prophages, we develop strains that can produce 20.3 g/L butyl acetate and 1.6 g/L butyl butyrate. Techno-economic analysis results suggest the economic competitiveness of our developed bioprocess. Our principles of selecting the most appropriate host for specific bioproduction and engineering microbial chassis to produce high-value and easy-separable end products may be applicable to other bioprocesses.


Assuntos
Acetatos/metabolismo , Butiratos/química , Clostridium/metabolismo , Ácidos Graxos/metabolismo , Fermentação/genética , Engenharia Metabólica/métodos , Acetilcoenzima A/metabolismo , Biocombustíveis/microbiologia , Biomassa , Clostridium/enzimologia , Clostridium/genética , Ésteres/metabolismo , Redes e Vias Metabólicas/genética , NAD/metabolismo , Proteínas/genética , Proteínas/metabolismo , Proteínas Recombinantes
10.
Front Psychol ; 12: 632461, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34262501

RESUMO

Perceived stress, Internet addiction and procrastination are common issues among college students. Based on the Compensatory Internet Use (CIU) model and emotion regulation theory, this study aims to explore two possible mechanisms for the connection between perceived stress and Internet addiction: mediating effect of procrastination and moderating effect of flow experience on the Internet. Cross-sectional design and questionnaire survey were used in this study. Data were collected from 446 college students who voluntarily completed self-reporting of perceived stress, internet addiction, procrastination and flow. Potential relationship structure and moderation model between variables was calculated during the process. The results revealed that there were significant associations among perceived stress, Internet addiction, procrastination and flow. The results also showed that procrastination plays a mediating role between perceptual stress and Internet addiction, flow plays a moderating role between them. The results emphasized the importance of the intention behind college students' overuse of the Internet. These results also provided a perspective of finding the possible causes of Internet addiction in college students, that is, individuals use the Internet to avoid stress and procrastinate, and the mobile experience on the Internet also affects the process.

11.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 35(4): 458-463, 2021 Apr 15.
Artigo em Chinês | MEDLINE | ID: mdl-33855830

RESUMO

Objective: To investigate the efficacy and safety of over-bending rod reduction and fixation technique via posterior approach in the treatment of unstable fresh thoracolumbar burst fracture. Methods: A clinical data of 27 patients with unstable fresh thoracolumbar burst fracture, who were met the inclusive criteria and admitted between January 2018 and October 2019, was retrospectively analyzed. There were 15 males and 12 females with an average age of 41.8 years (range, 26-64 years). The fractures were caused by falling from height in 14 cases, traffic accident in 8 cases, and crushing by a heavy objective in 5 cases. The interval between injury and operation was 1-7 days (mean, 3.2 days). The injured fracture was located at T 10 in 1 case, T 11 in 3 cases, T 12 in 6 cases, L 1 in 7 cases, L 2 in 7 cases, and L 3 in 3 cases. According to AO classification, there were 11 cases of type A3, 7 cases of type B, and 9 cases of type C. Neurological function was rated as grade A in 3 cases, grade B in 7 cases, grade C in 5 cases, and grade D in 12 cases according to the American Spinal Injury Association (ASIA) grading. All cases were treated by over-bending rod reduction and fixation technique via posterior approach, and 16 cases were combined with limited fenestration decompression. The evaluation indicators consisted of operation time, intraoperative blood loss, the compression ratio of the anterior vertebral height, the invasion rate of the injured vertebra into the spinal canal, the Cobb angle of segmental kyphosis, visual analogue scale (VAS) score, and Oswestry Disability Index (ODI). Results: The operation time was 67-128 minutes (mean, 81.6 minutes), and the intraoperative blood loss was 105-295 mL (mean, 210 mL). All patients were followed up 12-23 months (mean, 17.2 months). A total of 178 pedicle screws were implanted during operation, and the accuracy of the implantation was 98.9% (176/178). The compression ratios of the anterior vertebral height at the early postoperatively and last follow-up were significantly increased when compared with preoperative one ( P<0.05), and the invasion rate of the injured vertebra into the spinal canal, Cobb angle, VAS score, and ODI were significantly lower than those preoperatively ( P<0.05). Except that the ODI at last follow-up was significantly lower than that of the early postoperative period ( P<0.05), there was no significant difference between the last follow-up and the early postoperative period for other indicators ( P>0.05). At last follow-up, the neurological function was rated as grade A in 1 case, grade B in 2 cases, grade C in 4 cases, grade D in 9 cases, and grade E in 11 cases according to the ASIA grading, showing significant difference when compared with that before operation ( Z=-3.446, P=0.001). Conclusion: Over-bending rod reduction and fixation technique can effectively restore vertebral height, reset the invaded vertebral block, and selectively perform limited decompression and posterolateral bone grafting to ensure the completeness of intravertebral decompression and stability, which is one of the effective methods to treat unstable fresh thoracolumbar burst vertebral fracture.


Assuntos
Fraturas da Coluna Vertebral , Vértebras Torácicas , Adulto , Descompressão Cirúrgica , Feminino , Fixação Interna de Fraturas , Humanos , Vértebras Lombares/lesões , Vértebras Lombares/cirurgia , Masculino , Estudos Retrospectivos , Fraturas da Coluna Vertebral/cirurgia , Vértebras Torácicas/lesões , Vértebras Torácicas/cirurgia , Resultado do Tratamento
12.
Ann Transl Med ; 9(5): 396, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33842617

RESUMO

Background: Peroxisomes are ubiquitous and dynamic organelles that are involved in the metabolism of reactive oxygen species (ROS) and lipids. However, whether genetic variants in the peroxisome pathway genes are associated with survival in patients with melanoma has not been established. Therefore, our aim was to identify additional genetic variants in the peroxisome pathway that may provide new prognostic biomarkers for cutaneous melanoma (CM). Methods: We assessed the associations between 8,397 common single-nucleotide polymorphisms (SNPs) in 88 peroxisome pathway genes and CM disease-specific survival (CMSS) in a two-stage analysis. For the discovery, we extracted the data from a published genome-wide association study from The University of Texas MD Anderson Cancer Center (MDACC). We then replicated the results in another dataset from the Nurse Health Study (NHS)/Health Professionals Follow-up Study (HPFS). Results: Overall, 95 (11.1%) patients in the MDACC dataset and 48 (11.7%) patients in the NHS/HPFS dataset died of CM. We found 27 significant SNPs in the peroxisome pathway genes to be associated with CMSS in both datasets after multiple comparison correction using the Bayesian false-discovery probability method. In stepwise Cox proportional hazards regression analysis, with adjustment for other covariates and previously published SNPs in the MDACC dataset, we identified 2 independent SNPs (TMEM135 rs567403 C>G and PEX5 rs7969508 A>G) that predicted CMSS (P=0.003 and 0.031, respectively, in an additive genetic model). The expression quantitative trait loci analysis further revealed that the TMEM135 rs567403 GG and PEX5 rs7969508 GG genotypes were associated with increased and decreased levels of mRNA expression of their genes, respectively. Conclusions: Once our findings are replicated by other investigators, these genetic variants may serve as novel biomarkers for the prediction of survival in patients with CM.

13.
Seizure ; 88: 36-44, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33812306

RESUMO

OBJECTIVE: We aimed to develop and validate a predictive model of posttraumatic epilepsy (PTE). METHODS: The training cohort was patients registered at West China Hospital and diagnosed as traumatic brain injury (TBI) between January 1, 2011, and December 31, 2017. On the basis of multivariable cox proportional hazards model using a forward stepwise method, the nomogram was generated. We externally validated this instrument in 834 participants from two independent cohorts to assess its performance. RESULTS: The nomogram was built based on the results of multivariable cox proportional hazards regression analysis of 1301patients from West China Hospital. The prevalence of PTE was 12.8% (95% confidence interval [CI], 10.9-14.6%) in training cohort, 10.5% (95% CI, 7.5-13.4%) in the testing 1 cohort, and 6.1% (95% CI, 3.7-8.4%) in the testing 2 cohort. 7 independent predictors of PTE composed the nomogram (sex, time of loss of consciousness, subdural hemorrhage, contusion sites, early posttraumatic seizures, TBI severity, and treatment). The C-index was 0.846 (95% CI, 0.817-0.876), and the corresponding sensitivity and specificity were 0.867 and 0.738. External validations showed good discrimination in overall testing cohorts with a C-index of 0.895 (95% CI, 0.859-0.930), in the testing 1 cohort (C-index 0.897, 95% CI, 0.855-0.938) and testing 2 cohort (C-index, 0.883, 95% CI, 0.814-0.952). Calibration of this model was also good since the calibration plots were close to the ideal line. CONCLUSIONS: This nomogram was developed and validated in a large cohort for individualized prediction of PTE, which can identify individuals at high risk of epilepsy and help us find preventive drugs based on these targeted population.


Assuntos
Epilepsia Pós-Traumática , Epilepsia , China/epidemiologia , Epilepsia/diagnóstico , Epilepsia/epidemiologia , Epilepsia/etiologia , Humanos , Nomogramas , Estudos Retrospectivos
15.
Spectrochim Acta A Mol Biomol Spectrosc ; 252: 119492, 2021 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-33517216

RESUMO

Phthalic acid eaters (PAEs) play the role of plasticizer and have been widely used in the industrial and plastic production process. But due to not chemically bound in the polymeric matrix, PAEs can be easily released directly and/or indirectly into the environment, and pose a threat the ecosystem and human health. Small-molecule self-assembled nanoparticles have drawn more and more attention due to advantages of precise molecular structure, biocompatibility, great diversity, and tunability in optical properties and functionalities. Here we report the use of disaggregation-induced emission (DIE) based supramolecular assembly to design organic nanoprobe for detection PAEs. In the water solution, the designed small organic fluorophore AJ-1 was aggregated via noncovalent forces to form fluorescence off nanoparticles, but in the presence of PAEs, they disaggregated and produced a clear light-up fluorescent signal. The detection of PAEs with selectivity, sensitivity and rapid response were further achieved. The experiment of recovery of PAEs in real-water sample illustrated the practicability of probe AJ-1 in real-world applications. Besides, cellular uptake assay suggested that AJ-1 could pass through membrane and gather in the cytoplasm.


Assuntos
Corantes Fluorescentes , Nanopartículas , Ácidos Ftálicos , Compostos de Boro , Ecossistema , Humanos
16.
Nat Plants ; 7(1): 73-86, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33452487

RESUMO

Symbiosis between soybean (Glycine max) and rhizobia is essential for efficient nitrogen fixation. Rhizobial effectors secreted through the type-III secretion system are key for mediating the interactions between plants and rhizobia, but the molecular mechanism remains largely unknown. Here, our genome-wide association study for nodule number identified G. max Nodule Number Locus 1 (GmNNL1), which encodes a new R protein. GmNNL1 directly interacts with the nodulation outer protein P (NopP) effector from Bradyrhizobium USDA110 to trigger immunity and inhibit nodulation through root hair infection. The insertion of a 179 bp short interspersed nuclear element (SINE)-like transposon into GmNNL1 leads to the loss of function of GmNNL1, enabling bradyrhizobia to successfully nodulate soybeans through the root hair infection route and enhancing nitrogen fixation. Our findings provide important insights into the coevolution of soybean-bradyrhizobia compatibility and offer a way to design new legume-rhizobia interactions for efficient symbiotic nitrogen fixation.


Assuntos
Bradyrhizobium/metabolismo , Proteínas de Plantas/fisiologia , Raízes de Plantas/microbiologia , Soja/fisiologia , Simbiose/fisiologia , Bradyrhizobium/fisiologia , Estudo de Associação Genômica Ampla , Haplótipos/genética , Fixação de Nitrogênio , Proteínas de Plantas/genética , Raízes de Plantas/fisiologia , Polimorfismo de Nucleotídeo Único/genética , Nódulos Radiculares de Plantas/microbiologia , Nódulos Radiculares de Plantas/fisiologia , Soja/genética , Soja/microbiologia , Sequenciamento Completo do Genoma
17.
Spectrochim Acta A Mol Biomol Spectrosc ; 250: 119340, 2021 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-33422881

RESUMO

Fluorescence quenching of carbon dots (CDs) occurs in their aggregated state ascribed to direct π-π interactions or excessive resonance energy transfer (RET). Thus, CDs have been severely restricted for applications requiring phosphors that emit in the solid state, such as the fabrication of white light-emitting diodes (WLEDs). In this report, novel CDs with bright solid-state fluorescence (SSF) were synthesized by simple microwave-assisted synthesis method, using 1,4,7,10-tetraazacyclododecane (cyclen) and citric acid as precursors. Under 365 nm UV light, these CDs emit bright yellow SSF, indicating they successfully overcome the aggregation-induced fluorescence quenching (ACQ) effect. When the excitation wavelength (λex) is fixed at 450 nm, the emission peak of the CDs is centered at 546 nm with the Commission Internationale de l'Eclairage chromaticity (CIE) coordinates of (0.43, 0.55), which means that they can be combined with a blue-emitting chip in order to fabricate WLEDs. More importantly, the absolute quantum yield (QY) of these CDs powder reached 48% at λex of 450 nm, which was much higher than many previously reported SSF-emitting CDs and indicating their high light conversion ability in solid-state. Thanks to the excellent optical property of these CDs powder, they were successfully used in the preparation of high-performance WLEDs. This study not only enriches SSF-emitting CD-based nanomaterials with good prospects for application, but also provides valuable reference for subsequent research on the synthesis of solid-state fluorescent CDs.

18.
Biol Trace Elem Res ; 199(7): 2573-2581, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32959337

RESUMO

Relationships of lead exposure with blood pressure and blood lead with inflammation have been previously established yet, but the conclusions are still controversial. The objective of our study was to investigate the role of systemic inflammation in the relationships between blood lead concentration and blood pressure. We quantified the levels of blood lead and white blood cell count in 505 lead-exposed workers with 842 observations. Associations between blood lead, white blood cell count, and blood pressure were evaluated by using linear mixed models. We further performed mediation analysis to investigate the role of white blood cell count in the associations between blood lead concentration and blood pressure. We observed that each 1% increase of blood lead levels was significantly positively associated with a 0.9%, 1.7%, and 1.1% increases in systolic blood pressure, white blood cell count, and blood platelet count, respectively. Also, we found that the levels of white blood cell count were positively correlated with diastolic blood pressure and systolic blood pressure in a dose-response manner. Mediation analysis showed that the levels of white blood cell significantly mediated the associations between concentration of blood lead and systolic blood pressure. Collectively, our findings suggest that blood lead was positively associated with systolic blood pressure and that systemic inflammation might play a key role in this association.


Assuntos
Inflamação , Chumbo , Pressão Sanguínea , Humanos , Inflamação/induzido quimicamente , Chumbo/toxicidade , Contagem de Leucócitos
19.
Brain Res ; 1751: 147200, 2021 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-33166509

RESUMO

A considerable portion of patients with well-controlled seizures and visually normal brain structures experience seizure recurrence after anti-seizure medication is withdrawn. Microstructural abnormalities of the cortex may play an essential role in epilepsy relapse. Patients with idiopathic/cryptogenic epilepsy were registered. At the follow-up endpoint, 18 patients with relapse (PR+ group), 20 patients without relapse (PR- group), and 30 healthy controls were included. High-resolution T1-weighted images were obtained at the time of drug withdrawal. Microstructural features including cortical thickness, surface area, cortical volume and mean curvature in 68 cortical areas were calculated. A general linear model was applied to investigate intergroup differences, and then post hoc analysis was performed. Additionally, factor analysis was conducted to extract components from imaging measures showing a difference between PR- and PR+ groups, and independent associations between components and epilepsy relapse were assessed using a logistic regression model. Cortical thickness of the left paracentral lobule, left temporal pole and right superior frontal gyrus; surface area of the bilateral lingual gyrus and bilateral pericalcarine cortex; and cortical volume of the bilateral pericalcarine cortex had significant intergroup differences (false discovery rate correction, P < 0.05). All measures, except for cortical thickness of the left temporal pole, showed differences between PR- and PR+ groups. Two dominant components were extracted from these measures, and both were independently associated with epilepsy relapse. In conclusion, epilepsy patients with relapse presented distinct microstructural features of cortex at the time of drug withdrawal, which may serve as a potential biomarker for predicting seizure recurrence.

20.
Mol Plant ; 14(3): 488-502, 2021 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-33359013

RESUMO

Legumes establish symbiotic associations with rhizobia for biological nitrogen fixation. This process is highly regulated by various abiotic stresses, but the underlying genetic and molecular mechanisms remain largely unknown. In this study, we discovered that the glycogen synthase kinase 3 (GSK3)-like kinase, GmSK2-8, plays an important role in inhibiting symbiotic signaling and nodule formation in soybean (Glycine max) under salt stress. We found that GmSK2-8 is strongly induced in soybean under high-salt conditions, while GmSK2-8 could interact with two G. max Nodulation Signaling Pathway 1 (GmNSP1) proteins, GmNSP1a and GmNSP1b; these key transcription factors are essential for rhizobial infection, nodule initiation, and symbiotic gene expression in soybean. Furthermore, we demonstrated that GmSK2-8 phosphorylates the LHRI domain of GmNSP1a, inhibits its binding to the promoters of symbiotic genes, and thus suppresses nodule formation under salt stress. Knockdown of GmSK2-8 and its close homologs also resulted in reduced plant sensitivity to salt stress during nodule formation. Taken together, our findings indicate that GSK3-like kinases directly regulate the activities of GmNSP1s to mediate salt-inhibited legume-rhizobium symbiosis, providing novel targets for improving symbiotic nitrogen fixation under environmental stress conditions in soybean and possibly other legumes.


Assuntos
Fabaceae/enzimologia , Fabaceae/metabolismo , Quinase 3 da Glicogênio Sintase/metabolismo , Rhizobium/fisiologia , Soja/enzimologia , Soja/metabolismo , Regulação da Expressão Gênica de Plantas/genética , Regulação da Expressão Gênica de Plantas/fisiologia , Quinase 3 da Glicogênio Sintase/genética , Fixação de Nitrogênio/genética , Fixação de Nitrogênio/fisiologia , Fosforilação/genética , Fosforilação/fisiologia , Nodulação/genética , Nodulação/fisiologia , Regiões Promotoras Genéticas/genética , Transdução de Sinais/genética , Transdução de Sinais/fisiologia , Simbiose/genética , Simbiose/fisiologia
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