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1.
Nat Commun ; 10(1): 3941, 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31477699

RESUMO

Liquid biopsies have the utility for detecting minimal residual disease in several cancer types. Here, we investigate if liquid biopsy tracking on-treatment informs on tumour phenotypes by longitudinally quantifying circulating Epstein-barr virus (EBV) DNA copy number in 673 nasopharyngeal carcinoma patients undergoing radical induction chemotherapy (IC) and chemo-radiotherapy (CRT). We observe significant inter-patient heterogeneity in viral copy number clearance that is classifiable into eight distinct patterns based on clearance kinetics and bounce occurrence, including a substantial proportion of complete responders (≈30%) to only one IC cycle. Using a supervised statistical clustering of disease relapse risks, we further bin these eight subgroups into four prognostic phenotypes (early responders, intermediate responders, late responders, and treatment resistant) that are correlated with efficacy of chemotherapy intensity. Taken together, we show that real-time monitoring of liquid biopsy response adds prognostic information, and has the potential utility for risk-adapted treatment de-intensification/intensification in nasopharyngeal carcinoma.

2.
Anim Cogn ; 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31506795

RESUMO

Species facing similar selection pressures should recognize heterospecific alarm signals. However, no study has so far examined heterospecific alarm-call recognition in response to parasitism by cuckoos. In this study, we tested whether two sympatric host species of the common cuckoo Cuculus canorus, Oriental reed warbler Acrocephalus orientalis (ORW, main host), and black-browed reed warbler Acrocephalus bistrigiceps (BRW, rare host), could recognize each other's alarm calls in response to cuckoos. Dummies of common cuckoo (parasite) and Eurasian sparrowhawk Accipiter nisus (predator) were used to induce and record alarm calls of the two warbler species, respectively. In the conspecific alarm-call playback experiments, ORW responded more strongly to cuckoo alarm calls than to sparrowhawk alarm calls, while BRW responded less strongly to cuckoo alarm calls than to sparrowhawk alarm calls. In the heterospecific alarm-call playback experiments, both ORW and BRW responded less strongly to cuckoo alarm calls than sparrowhawk alarm calls. BRW seemed to learn the association between parasite-related alarm calls of the ORW and the cuckoo by observing the process of ORW attacking cuckoos. In contrast, alarm calls of BRW to cuckoos were rarely recorded in most cases. BRW with low parasite pressure still developed recognition of heterospecific parasite-related alarm call. Unintended receivers in the same community should recognize heterospecific alarm calls precisely to extract valuable information.

3.
J Environ Manage ; 248: 109310, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31376615

RESUMO

Microbial fuel cell (MFC) was compared to conventional biological techniques for decolorization of anthraquinone dye, reactive blue 19 (RB19) with simultaneous electricity generation. With 50 mg/L of RB19 in the anode chamber as a fuel, the MFC achieved 89% decolorization efficiency of RB19 within 48 h, compared with 51 and 55% decolorization efficiency achieved by aerobic and anaerobic techniques, respectively. The cyclic voltammetry results showed that RB19 could promote the electron transfer and redox reaction on the surface of anode. The RB19 decolorization process can be described by first-order kinetics, and the decolorization rate decreased with the increase of RB19 concentration. The high-throughput 16S rRNA sequencing analysis indicated significant microbial community shift in the MFC. At phylum level, the majority of sequences belong to Proteobacteria, accounting from 23 to 84% of the total reads in each bacterium community. At genus level, the MFC contained two types of microorganisms in general such as electrochemically active and decolorization bacteria. Overall, MFC is an effective method for anthraquinone dye treatment with simultaneous energy recovery. The 16S rRNA revealed that there were two major functioning microbial communities in the MFC such as electricity-producing and RB19-degrading bacteria which synergistically worked on RB19 degradation.

4.
Br J Radiol ; : 20190271, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31453720

RESUMO

OBJECTIVE: Radiomics pipelines have been developed to extract novel information from radiological images, which may help in phenotypic profiling of tumours that would correlate to prognosis. Here, we compared two publicly available pipelines for radiomics analyses on head and neck CT and MRI in nasopharynx cancer (NPC). METHODS AND MATERIALS: 100 biopsy-proven NPC cases stratified by T- and N-categories were enrolled in this study. Two radiomics pipeline, Moddicom (v. 0.51) and Pyradiomics (v. 2.1.2) were used to extract radiomics features of CT and MRI. Segmentation of primary gross tumour volume was performed using Velocity v. 4.0 by consensus agreement between three radiation oncologists. Intraclass correlation between common features of the two pipelines was analysed by Spearman's rank correlation. Unsupervised hierarchical clustering was used to determine association between radiomics features and clinical parameters. RESULTS: We observed a high proportion of correlated features in the CT data set, but not for MRI; 76.1% (51 of 67 common between Moddicom and Pyradiomics) of CT features and 28.6% (20 of 70 common) of MRI features were significantly correlated. Of these, 100% were shape-related for both CT and MRI, 100 and 23.5% were first-order-related, 61.9 and 19.0% were texture-related, respectively. This interpipeline heterogeneity affected the downstream clustering with known prognostic clinical parameters of cTN-status and GTVp. Nonetheless, shape features were the most reproducible predictors of clinical parameters among the different radiomics modules. CONCLUSION: Here, we highlighted significant heterogeneity between two publicly available radiomics pipelines that could affect the downstream association with prognostic clinical factors in NPC. ADVANCES IN KNOWLEDGE: The present study emphasized the broader importance of selecting stable radiomics features for disease phenotyping, and it is necessary prior to any investigation of multicentre imaging datasets to validate the stability of CT-related radiomics features for clinical prognostication.

5.
Inorg Chem ; 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31441302

RESUMO

We present a combined strategy of experiments and theoretical modeling for understanding the evolution of the morphology and plasmonic properties of gold nanostars (GNSs) in the seed-mediated synthesis by changing the poly(vinylpyrrolidone) (PVP) molecular weight, PVP concentration, and synthesis temperature. A dramatic change of the morphology of GNSs as a function of these synthesis parameters is observed that is related to variations of the plasmonic properties and thus surface-enhanced Raman spectroscopy (SERS) enhancement. We observe the favorable growth of anisotropic GNS structures with sharp protruding tips using PVP of low molecular weight and of rounded GNSs with short protruding tips using PVP of high molecular weight. The PVP concentration has less influence on the core size than on the tip length of GNSs. The high synthesis temperature causes the rounding of the GNS structure. Finite-difference time-domain (FDTD) simulations reveal a remarkable correlation of the GNS morphology with the plasmonic properties as well as the SERS enhancement. The maximum local electric field enhancement occurs at the apex of the sharp protruding tips of the GNSs. The weak plasmonic coupling is observed between the protruding tips of GNSs because of their large separation distance, and increasing the number of protruding tips beyond two only increases the extinction cross section without further red-shifting the plasmon peak. A resonance overlap of the plasmon band with the incident laser wavelength is responsible for the morphology-dependent plasmonic properties and SERS enhancement. The present work demonstrates that a mechanistic understanding of the structural evolution of GNSs along with their morphology-plasmonic property correlation can be achieved through the combination of experimental investigations and FDTD-based theoretical modeling.

6.
J Histochem Cytochem ; : 22155419871550, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31424999

RESUMO

The aim of this study was to investigate the expression of the activating transcription factor 4 (ATF4) in odontogenic keratocysts (OKC), its association with hypoxia and M2-polarized macrophages infiltration, and its potential relationships with angiogenesis in OKC. The expression of ATF4, hypoxia-inducible factor 1α (HIF-1α), macrophage colony-stimulating factor (M-CSF), and receptor activator of nuclear factor κ-B ligand (RANKL) in OKC samples and normal oral mucosa (OM) was detected by immunohistochemistry. Meanwhile, microvessel density (MVD) was measured using antibody against CD31. M2-polarized macrophages were identified using double-staining for CD68+ and CD163+. The correlations of ATF4 with HIF-1α, M-CSF, and M2-polarized macrophages infiltration were determined by Spearman's rank correlation test and hierarchical clustering. Human immortalized oral epithelial cells (HIOECs) were used in in vitro experiments. Our data showed that the expression of HIF-1α, ATF4, and M-CSF was significantly upregulated in the epithelium of OKC when compared with the OM. The expression of ATF4 was positively correlated with that of HIF-1α, M-CSF, MVD, and M2-polarized macrophages infiltration. Elevated expression of ATF4 in the epithelial lining of OKC may facilitate the M2 macrophages infiltration in response to hypoxia, leading to the development of OKC.

7.
Nat Neurosci ; 2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31451801

RESUMO

Comorbid depressive symptoms (CDS) in chronic pain are a common health problem, but the neural circuit mechanisms underlying these symptoms remain unclear. Here we identify a novel pathway involving 5-hydroxytryptamine (5-HT) projections from the dorsal raphe nucleus (5-HTDRN) to somatostatin (SOM)-expressing and non-SOM interneurons in the central nucleus of the amygdala (CeA). The SOMCeA neurons project directly to the lateral habenula, an area known involved in depression. Inhibition of the 5-HTDRN→SOMCeA pathway produced depression-like behavior in a male mouse model of chronic pain. Activation of this pathway using pharmacological or optogenetic approaches reduced depression-like behavior in these mice. Human functional magnetic resonance imaging data showed that compared to healthy controls, functional connectivity between the CeA-containing centromedial amygdala and the DRN was reduced in patients with CDS but not in patients in chronic pain without depression. These findings indicate that a novel 5-HTDRN→SOMCeA→lateral habenula pathway may mediate at least some aspects of CDS.

8.
PLoS Biol ; 17(8): e3000417, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31469831

RESUMO

Threatening sounds can elicit a series of defensive behavioral reactions in animals for survival, but the underlying neural substrates are not fully understood. Here, we demonstrate a previously unexplored neural pathway in mice that projects directly from the auditory cortex (ACx) to the lateral periaqueductal gray (lPAG) and controls noise-evoked defensive behaviors. Electrophysiological recordings showed that the lPAG could be excited by a loud noise that induced an escape-like behavior. Trans-synaptic viral tracing showed that a great number of glutamatergic neurons, rather than GABAergic neurons, in the lPAG were directly innervated by those in layer V of the ACx. Activation of this pathway by optogenetic manipulations produced a behavior in mice that mimicked the noise-evoked escape, whereas inhibition of the pathway reduced this behavior. Therefore, our newly identified descending pathway is a novel neural substrate for noise-evoked escape and is involved in controlling the threat-related behavior.

9.
Soft Matter ; 15(33): 6690-6695, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31380555

RESUMO

An acylhydrazone-based colorimetric and fluorescent sensor (PAH-8) for the detection of fluoride ions (F-) and trifluoroacetic acid (TFA)/triethylamine (TEA) has been studied. PAH-8 solution and organogel are highly selective and sensitive to F- among various tested anions (F-, Cl-, Br-, AcO-, and H2PO4-) in DMSO. Upon addition of F-, the maximum absorption wavelength of PAH-8 in DMSO solution shows a big red shift from 377 nm to 464 nm with a marked color change from colorless to yellow, and the fluorescence emission also displays a red shift from 438 nm to 532 nm with its fluorescence emission switching from blue to yellow. Both Job's plot and the Benesi-Hildebrand plot confirm a 1 : 1 stoichiometric relationship between PAH-8 and F-. The detection limit of PAH-8 for the analysis of F- can reach 8.31 × 10-7 M. Very interestingly, an expeditious 'naked eye' detection of F- can be realized by the reversible gel-sol transition, along with a color change from slight yellow to bright red and fluorescence quenching. In addition, the cast film of PAH-8 (the solution was developed from the DMSO gel upon the addition of TBAF) can detect water in air by color switching or fluorescence on/off. In both solution and solid states, PAH-8 also exhibits obvious acid-base stimulated fluorescence conversion. The solution of PAH-8 can convert the blue emission into intensive sky blue emission after adding trifluoroacetic acid (TFA), and the solid film exhibits no fluorescence after fuming with TFA vapors, while fuming with triethylamine (TEA) vapors resulted in intensive green emission.

10.
Bioresour Technol ; 291: 121894, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31387839

RESUMO

Microalgae accumulate starch and lipid as storage metabolites under nutrient depletion, which can be used as sustainable feedstock for biorefinery. Omics analysis coupled with enzymatic and genetic verifications uncovered a partial picture of pathways and important enzymes or regulators related to starch and lipid biosynthesis as well as the carbon partitioning between them under nutrient depletion conditions. Depletion of macronutrients (N, P, and S) resulted in considerable enhancement of starch and/or lipid content in microalgae, but the accompanying declined photosynthesis hampered the achievements of high concentrations. This review summarized the current knowledge on the pathways and the committed steps as well as their carbon allocation involved in starch and lipid biosynthesis, and focused on the manipulation of different nutrients and the alleviation of oxidative stress for enhanced storage metabolites production. The biological and engineering approaches to cope with the conflict between biomass production and storage metabolites accumulation are proposed.


Assuntos
Metabolismo dos Lipídeos , Microalgas/metabolismo , Nutrientes , Amido/metabolismo , Biomassa , Lipídeos
11.
ACS Appl Mater Interfaces ; 11(33): 30384-30390, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31347825

RESUMO

Constructing high capacitance active materials and three-dimensional (3D) conductive networks inside textile yarn frames is a promising strategy to synthesize yarn supercapacitor electrodes. In this study, growing NiCo2S4@Ni-Co layered double hydroxide (LDH) nanotube arrays on Au-metalized cotton yarns yields a novel yarn supercapacitor electrode material. The resulting yarn electrode possesses numerous merits, including high electrical conductivity from NiCo2S4 and Au-metalized cotton yarns, high capacitance of Ni-Co LDH nanosheets, and the 3D hierarchical electrode structure. The unique electrode structure leads to excellent electrochemical properties including high capacitance (5680 mF cm-2), excellent rate performance, and stable cycling performance. A two-ply symmetric yarn supercapacitor assembled from the NiCo2S4/Ni-Co LDH/Au/cotton yarn electrode reaches an areal energy density of 3.5 µW h cm-2.

12.
Medicine (Baltimore) ; 98(26): e15946, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31261499

RESUMO

Studies investigating the association between transforming growth factor (TGF-ß-509C/T, rs1800469) promoter polymorphism and myocardial infarction (MI) risk reported inconsistent results. The aim of our study was to assess the association between the 509C/T polymorphism of the TGF-ß gene (rs1800469) and MI risk.A total of 5460 cases and 8413 controls in 7 case-control studies were incorporated in our current meta-analysis. The original studies were selected through searching the databases of the PubMed and EMBASE. The odds ratio (OR) and 95% confidence interval (95% CI) of TGF-ß 509C/T (rs1800469) for MI risk were applied to estimate the strength of the association.Our results showed that T allele carriers had a 13% increased risk of MI, when compared with the C allele carriers (OR = 1.13, 95% CI: 1.00-1.27). In the subset analysis by the type of MI, significantly elevated risk of MI was associated with the homozygote TT and heterozygote C/T in no-AMI subjects, when compared with the CC homozygote carriers (OR = 1.12, 95% CI:1.02-1.23).Our meta-analysis shows that the polymorphism with homozygote TT and heterozygote C/T of TGF-ß 509C/T (rs1800469) is significantly associated with the increased risk of MI.


Assuntos
Predisposição Genética para Doença , Infarto do Miocárdio/genética , Polimorfismo de Nucleotídeo Único , Fator de Crescimento Transformador beta/genética , Humanos
13.
Urol Int ; 103(2): 187-194, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31170710

RESUMO

OBJECTIVES: This meta-analysis was to assess solifenacin and tolterodine in patients with overactive bladder. METHODS: We searched PubMed, the Cochrane Library, EMBASE, CNKI, Wanfang, and ClinicalTrials.gov databases for randomized controlled trials (RCTs). The efficacy endpoint was daily micturition frequency, daily urgency episodes, daily incontinence episodes, and micturition volume per voiding. The safety endpoint was the incidence of the rate of major bleeding, intracranial bleeding, and gastrointestinal bleeding. RESULTS: Seven RCTs met the inclusion criteria and 1,318 patients were included. The meta-analysis showed that, compared with tolterodine, solifenacin was associated with similar daily micturition frequency, daily urgency episodes, daily incontinence episode, and micturition volume per voiding at 8 and 12 weeks of follow-up. Moreover, no significant difference was obtained in the incidence of dry mouth between solifenacin and tolterodine at 8 and 12 weeks of follow-up. However, tolterodine decreased the constipation rate at 12 weeks compared with solifenacin. CONCLUSION: Solifenacin and tolterodine yielded similar results on daily micturition frequency, daily urgency episodes, daily incontinence episodes, micturition volume per voiding, and the incidence of dry mouth. However, tolterodine can decrease the constipation rate at 12 weeks compared with solifenacin.

14.
J Colloid Interface Sci ; 553: 40-49, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31185382

RESUMO

HYPOTHESIS: Conventional strategies based on emulsion templates to produce porous materials are complicated and not environmental-friendly, thus inspiring a facile and novel approach via polymerization of aqueous foams. Graphene oxide (GO) has been proved as a very efficient Pickering stabilizer for oil-in-water or water-in-oil emulsions, however, it has rarely (if any) been used to stabilize aqueous foams. It is of high interest to prepare GO-stabilized aqueous foams and subsequent porous hydrogels after polymerization of the aqueous phase. EXPERIMENTS: GO was slightly hydrophobized with cetyltrimethylammonium bromide (CTAB) and then used to stabilize aqueous foams containing a water-soluble monomer, N-(3-Sulfopropyl)-N-methacroyloxyethyl-N,N-dimethylammonium betaine (SBMA). After polymerizing the aqueous phase, macroporous zwitterionic hydrogels (PSBMA) were prepared. The pore morphology of macroporous PSBMA were observed by a field-emission scanning electron microscope. FINDINGS: The wettability of modified GO by both water and air, indeed has a significant influence on the air bubble size and size distribution as well as the pore size of resulting macroporous PSBMA hydrogels. The pore size and pore interconnectivity of the hydrogels can be tailored by simply varying the surface property and concentration of GO in water. PSBMA hydrogels exhibit a pronounced uptake of aqueous NaCl solutions and efficient separation of oil-in-water emulsions.

15.
Environ Sci Technol ; 53(13): 7551-7563, 2019 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-31244058

RESUMO

Pd-catalyzed reduction has emerged as a promising treatment strategy to remove the recalcitrant disinfection byproduct N-nitrosodimethylamine (NDMA). However, the reaction pathways remain unexplored, and questions remain about how water solvent influences NDMA reduction mechanisms and selectivity. Here, we compute the energies and barriers of all relevant elementary steps in NDMA reduction by H2 on Pd(111) using density functional theory. We further calculate water-assisted H-shuttling for all hydrogenation reactions explicitly and include water solvation for all elementary reactions implicitly. We parametrize microkinetic models to predict product formation rates and selectivities over a wide range of NDMA concentrations. We show that H2O-mediated H-shuttling lowers the reaction barriers for all hydrogenation reactions involved in NDMA reduction while implicit solvation has negligible impact on the reaction and activation energies. We further conduct batch experiments with SiO2-supported Pd nanoparticles and compare them with the microkinetic models. The predicted rates, selectivity, and apparent activation energy from the model parametrized with both explicit H2O-mediated H-shuttling and implicit solvation correspond well with experimental observations. Models that ignore water as an H-shuttle or solvent fail to recover the experimental rates and apparent activation energy. We identified the rate-determining steps of the reaction and show the reaction flow pathways of the complicated reaction network. Finally, we demonstrate that water-mediated H-shuttling changes the rate-determining steps and reaction flows of elementary reactions.

16.
Food Chem ; 293: 387-395, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31151626

RESUMO

The endogenous carbon quantum dots (CQDs) produced during food processing have potential uncertainty to human health. The objective of this study was to investigate the formation, biodistribution and cytotoxicity of CQDs in roasted Atlantic salmon (Salmo salar Linnaeus). The X-ray photoelectron spectroscopy (XPS) analysis showed that the CQDs were mainly composed of carbon, oxygen and nitrogen. The morphology, functional groups and optical properties were highly dependent on the roasting time. In vivo experiments in mice demonstrated that the CQDs distributed in the digestive tract, kidney, liver, and even brain, which indicated that they could cross the blood-brain barrier. The cell imaging results indicated that the CQDs could readily gain access to the normal rat kidney (NRK) cells, and caused autophagosome formation. The proportion of live cells decreased to 34.62% at 6 mg mL-1 of CQDs, and the energy generation route was changed from aerobic to glycolytic metabolism.


Assuntos
Pontos Quânticos/química , Pontos Quânticos/toxicidade , Salmo salar , Animais , Apoptose/efeitos dos fármacos , Autofagossomos/efeitos dos fármacos , Autofagossomos/metabolismo , Barreira Hematoencefálica/efeitos dos fármacos , Carbono/análise , Ciclo Celular/efeitos dos fármacos , Linhagem Celular , Culinária , Masculino , Camundongos Endogâmicos BALB C , Nitrogênio/análise , Oxigênio/análise , Espectroscopia Fotoeletrônica , Pontos Quânticos/metabolismo , Ratos , Distribuição Tecidual
17.
Am J Med Genet A ; 179(9): 1764-1777, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31240838

RESUMO

Diffuse idiopathic skeletal hyperostosis (DISH) is a disorder principally characterized by calcification and ossification of spinal ligaments and entheses. Fibrodysplasia ossificans progressiva (FOP) is a rare autosomal dominant disabling disorder characterized by progressive ossification of skeletal muscle, fascia, tendons, and ligaments. These conditions manifest phenotypic overlap in the ossification of tendons and ligaments. We describe herein a patient with DISH, exhibiting heterotopic ossification of the posterior longitudinal ligament where clinical whole exome sequencing identified a variant within ACVR1, a gene implicated in FOP. This variant, p.K400E, is a novel variant, not identified previously, and occurs in a highly conserved region across orthologs. We used sequence-based predicative algorithms, molecular modeling, and molecular dynamics simulations, to test the potential for p.K400E to alter the structure and dynamics of ACVR1. We applied the same modeling and simulation methods to established FOP variants, to identify the detailed effects that they have on the ACVR1 protein, as well as to act as positive controls against which the effects of p.K400E could be evaluated. Our in silico molecular analyses support p.K400E as altering the behavior of ACVR1. In addition, functional testing to measure the effect of this variant on BMP-pSMAD 1/5/8 target genes was carried out which revealed this variant to cause increased ID1 and Msx2 expression compared with the wild-type receptor. This analysis supports the potential for the variant of uncertain significance to contribute to the patient's phenotype.

18.
J Cell Biochem ; 120(10): 17650-17661, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31190355

RESUMO

Small-cell lung cancer (SCLC) is an aggressive malignancy characterized by high cellular proliferation and early distant metastasis. Our study aimed to explore the effect of miR-22-3p (miR-22, for short) on SCLC radiosensitivity and its molecular mechanisms. The expression level of miR-22 was evaluated in a human normal lung epithelial cell line and a human SCLC cell line, and cell apoptosis and migration were detected. The expression of the miR-22 direct target WRNIP1 mRNA and protein were explored. Five differentially expressed genes were detected. The miR-22 expression in NCI-H446 was significantly decreased, and miR-22 overexpression significantly promoted cell apoptosis. miR-22 overexpression could significantly inhibit the cell migration of SCLC cells, and miR-22 had a negative regulatory effect on WRNIP1 mRNA and protein levels. KLK8 was downregulated, and the messenger RNA (mRNA) of four other genes (PC, SCUBE1, STC1, and GPM6A) was upregulated mRNA in cells overexpressing miR-22, which was in accordance with the bioinformatics analysis. miR-22 could enhance the radiosensitivity of SCLC by targeting WRNIP1.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31147708

RESUMO

The aim was to estimate the effective doses associated with different types of scanning protocols and how much the diagnostic computed tomography (DCT) scan contributed to the total dose of the dual-modality positron emission tomography/computed tomography (PET/CT) examinations. The results showed that an average radiation dose of 8.19 ± 0.83 mSv and 13.44 ± 5.14 mSv for the PET and CT components, respectively, resulting in a total dose of 21.64 ± 5.20 mSv. Approximately 92.7% (980 of 1057) of the patients underwent additional DCT protocols. The DCT protocols contributed 42% of the overall effective radiation doses, which was larger than the percentage contributed by the PET component (38%) and LCT protocols (20%). Reducing the diagnostic area of the DCT scans that patients undergo and decreasing the use of chest-abdomen-pelvis (CAP), abdomen-pelvis (AP) and chest DCT protocols, especially the CAP protocol, will be helpful in decreasing the effective radiation doses of PET/CT scan.

20.
J Cell Physiol ; 234(12): 22972-22984, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31134620

RESUMO

The function of microRNA-27a (miR-27a) on synovial angiogenesis and chondrocyte apoptosis in rats with knee osteoarthritis (KOA) by targeting PLK2 is explored in this present study. The rat model of KOA was conducted by anterior cruciate ligament transection. Rats were injected with miR-27a mimics, mimics NC, pcDNA3.1-PLK2 pcDNA3.1, or RLK2 RNAi plasmid via tail vein. A series of assays were used to figure out the functions of miR-27a and PLK2 in synovial angiogenesis and chondrocyte apoptosis in rats with KOA. Furthermore, the putative binding site between miR-27a and PLK2 was determined. Downregulated miR-27a was found in synovial tissues and cartilage tissues of KOA rats. Upregulated miR-27a and downregulated PLK2 inhibited synovial injury and promoted apoptosis of synovial cells, inhibited synovial angiogenesis, inhibited cartilage injury and chondrocyte apoptosis, inhibited cartilage collagen destruction, and alleviates inflammatory injury of synovial tissue and cartilage tissue in KOA rats. Overexpression of PLK2 reverses the effect of upregulation of miR-27a on synovial angiogenesis and chondrocyte injury in KOA rats. Our study suggests that upregulation of miR-27a inhibits synovial angiogenesis and chondrocyte apoptosis in KOA rats through the inhibition of PLK2.

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