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1.
J Hazard Mater ; 387: 122017, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31927259

RESUMO

Metal oxides with the hollow microstructure by the facile synthetic strategy are hopeful in applications for photocatalysis, supercapacitor, and gas sensor owing to their large surface areas, porosity ratio and rich active sites. In this work, indium oxide porous hollow rods (In2O3 PHRs) are successfully prepared using metal-organic frameworks (MOFs) as the template. The morphology of In2O3 PHRs is hexagonal hollow micro-rods with a porous structure. The investigation on the gas-sensing performance reveals that the In2O3 PHRs sensor displays outstanding sensitivity and selectivity toward 10 ppm chlorine gas (Cl2) at low operational temperature (160 °C). Furthermore, the In2O3 PHRs sensor displays a low detection limit (3.2 ppb) and short response and recovery time (38/13 s). The unique morphology and abundant oxygen vacancies are conduced to the excellent gas-sensing activities, which is benefited from the utilization and decomposition of In-MOFs precursor. In addition, the gas sensing mechanism of reducing gases and oxidizing gases is deduced in detail for the In2O3 PHRs sensor.

2.
Sci Total Environ ; 712: 136502, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31931197

RESUMO

What the extent of meteorological drought could trigger the corresponding hydrological drought with different levels? This question is an important topic in the field of drought propagation, which however has not been resolved. Therefore, a novel model based on a Bayesian network was proposed to address this issue in this study. In this model, the drought pooling and excluding methods were applied to eliminate minor drought events. A drought matching approach based on drought propagation time was proposed to achieve the one by one matching between different types of drought. Moreover, based on the matched drought events and the copula-based conditional probability model, the drought propagation thresholds of meteorological drought for triggering hydrological drought at various levels were determined. In addition, the interval conditional probability was calculated to further explore the sensitivity of hydrological drought response to different meteorological drought conditions. Furthermore, the propagation ratio was proposed to characterize the differences of drought propagation threshold among various regions. The Wei River Basin was selected as a case study. Results indicated that the results of drought propagation threshold were reliable and accurate. The increase of interval conditional probability showed a typical S-curve, which can intuitively obtain the probability of hydrological drought occurrence at different levels under specific meteorological drought condition, so as to effectively guide drought preparedness and mitigation. The propagation ratio can describe the overall resistance of the basin to meteorological drought, and it mainly depended on the meteorological and underlying surface conditions as well as groundwater supply.

3.
Sci Total Environ ; 713: 136583, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31955091

RESUMO

Polycarbonate (PC) and polyethylene terephthalate (PET) as the package materials have been widely used for Chinese bottled water, from which estrogenic bisphenol analogues might migrate into bottled water. Therefore, there is a strong need to investigate the occurrence and potential risk of such estrogenic bisphenol analogues in Chinese bottled waters. In this study, a GC-MS method was first established and validated for determination of trace-level ten kinds of bisphenol analogues, including bisphenol A (BPA), bisphenol B (BPB), bisphenol C (BPC), bisphenol E (BPE), bisphenol F (BPF), bisphenol P (BPP), bisphenol S (BPS), bisphenol Z (BPZ), bisphenol AP (BPAP), and bisphenol AF (BPAF). BPA was detected in all eleven brands of PET bottled waters with concentrations of 12.4-44.9 ng/L. Some bisphenol analogues were detected in PET bottled waters, and the average concentrations of BPA, BPE, and BPAF in PET bottled waters were found to be 20.8, 1.8, and 2.2 ng/L, respectively. The other eight bisphenol analogues were not detected in PET bottled waters. On the other hand, BPA was detected with high concentrations of 111.8 to 6452.8 ng/L in ten brands of PC bottled water. The average concentrations of BPA, BPS, BPAP, and BPAF were determined to be 1394.3, 1.9, 1.4 and 1.0 ng/L, respectively, while the other seven bisphenol analogues were not detected. High BPA concentration detected in PC bottled waters would remarkably increase human BPA daily intake through daily consumption of such bottled waters. Meanwhile, high estrogen equivalence (EEQ) in PC bottled waters of China is mainly due to the presence of BPA, which may imply adverse effect on human. Therefore, further investigation should be dedicated to assess PC bottled water-associated BPA risks in a more holistic manner.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31957946

RESUMO

Lead halide perovskites have attracted tremendous attention to find their promising potential in various applications as optoelectronics. Unfortunately, this materials with mixed cations/anions often suffer from phase segregation, which is detrimental to the device efficiency and long-term stability. During perovskite film growth, the gel stage (in between liquid and crystalline) correlates to the phase segregation, which has been rarely explored. Herein, we systematically investigate the cation diffusion kinetics at the gel stage to develop a diffusion model obeying Fick's Second Law. Take 2D layered perovskite as an example, we combine theoretical and experimental results to reveal the impacts of diffusion coefficient, temperature, and gel duration on the film growth and phase formation. In light of this finding, we successfully fabricate a homogenous 2D perovskite thin film without significant phase segregation. This in-depth understanding of gel stage and relevant cation diffusion kinetics would further guide the design and processing of halide perovskites with mixed composition to meet requirements for optoelectronic applications.

5.
Nat Prod Res ; : 1-6, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31951483

RESUMO

During the course of searching for structurally interesting and bioactive compounds, a further chemical investigation of the leaves of Heynea trijuga Roxburgh was performed, which led to the isolation of a new ergostane derivative, named 3ß, 4ß, 20S-trihydroxyergosta-5, 24(28)-dien-16-one (1), together with five known sterides (3ß, 23S)-ergosta-5, 24(28)-diene-3, 23-diol (2), ergosta-5, 24(28)-diene-3ß-diol (3), stigmast-5-ene-3ß, 7α-diol (4), sitoindoside I (5) and stigmast-3ß, 5α, 6ß-triol (6). The structure of the new compound was elucidated using a combination of 1 D, 2 D NMR techniques and HR-EI-MS analyses. All the compounds were evaluated for cytotoxic activity against tumor cell line BEL-7402 by MTT method.

6.
Nano Lett ; 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31940203

RESUMO

The in situ construction of nano-assembly has been demonstrated to improve the performance of bioactive molecules, but the control of the morphology of nanomaterials in vivo still remains a tremendous challenge. Herein, a photothermal-promoted morphology transformation (PMT) strategy is developed to accelerate the formation of nanomaterials for improving biological performance of drug molecules. Compared with the spontaneous process, the rate of transformation increases by ~4 times in PMT process. Owing to the raise of the assembly rate, the tumor accumulation of drugs is ~2-fold than that without photo irradiation, which inhibits the tumor growth effectively. More importantly, the chemical re-assembly process in vitro and in vivo is monitored by the advanced ratiometric photoacoustic image, confirming the photo-induced transformation acceleration. Through the noninvasively artificial control on assembly dynamics in vivo, the PMT strategy provides a new insight for developing the intelligent theranostics.

7.
Pest Manag Sci ; 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31944525

RESUMO

BACKGROUND: Apolygus lucorum (Meyer-Dür) is a serious worldwide agricultural pest, especially for Bt cotton in China. Pyrethroids, neonicotinoids and organophosphates are the most effective insecticides to control piercing and sucking insects including A.lucorum. The voltage-gated sodium channel (VGSC) is major target site of pyrethroids. Extensive alternative splicing and RNA editing, two major post-transcriptional mechanisms, contribute to generate different functional sodium channel variants. In our research, we characterized the sodium channel varients of A. lucorum. RESULTS: In this study, we isolated numerous sodium channel variants that cover the entire coding region of the VGSC gene from A. lucorum. All clones could be grouped into 47 splice types based on the presence of 9 alternative exons (exons j, n, o, a, p, b, s, q and t). Exon j, b and t were located independently, while Exon n, o, a and p were located adjacently, it 's same with exon s and q. We also found 35 nucleotide changes in different positions in individual variants, of which, 18 nucleotide changes were A-to-I RNA editing, 11 nucleotide changes were likely due to U-to-C or C-to-U editing, the others were likely natural sequence polymorphisms in the population. Futhermore, we expressed all of the variants in Xenopus oocytes, 18 of them were functional expressed in oocytes and sensitive to TTX. CONCLUSION: Our results provide a functional basis for understanding how A.lucorum sodium channel variants work in regulating channel expression, pharmacology and gating properties for agricultural insects. This article is protected by copyright. All rights reserved.

8.
Theranostics ; 10(1): 281-299, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31903120

RESUMO

RNA molecules (e.g., siRNA, microRNA, and mRNA) have shown tremendous potential for immunomodulation and cancer immunotherapy. They can activate both innate and adaptive immune system responses by silencing or upregulating immune-relevant genes. In addition, mRNA-based vaccines have recently been actively pursued and tested in cancer patients, as a form of treatment. Meanwhile, various nanomaterials have been developed to enhance RNA delivery to the tumor and immune cells. In this review article, we summarize recent advances in the development of RNA-based therapeutics and their applications in cancer immunotherapy. We also highlight the variety of nanoparticle platforms that have been used for RNA delivery to elicit anti-tumor immune responses. Finally, we provide our perspectives of potential challenges and opportunities of RNA-based nanotherapeutics in clinical translation towards cancer immunotherapy.

9.
ACS Nano ; 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31904225

RESUMO

Compared with a single nanowire (NW) or NW array, the simpler preparation process of an NW network (NWN) enables it to be fabricated in large-scale, flexible, and wearable applications of photodetectors (PDs). However, the NWN behaves many microinterfaces (MIs) between NWs, seriously limiting the device performance and stability. Here, we demonstrate a welding strategy for an MAPbI3 NWN, which enhances the crystallinity of the NWN and enhances the radial transmission of photogenerated carriers, leading to a better device performance with ultrahigh stability. Our NWN PDs fabricated by using the welding strategy showed ultrahigh performance with an on/off ratio and detectivity of 2.8 × 104 and 4.16 × 1012 Jones, respectively, which are the best performance for reported metal-semiconductor-metal (MSM) perovskite NWN PDs and are comparable to those of single-NW or NW array PDs. More importantly, our unpackaged NWN PDs show ultrahigh storage stability in air with a humidity of 55-65%, and the flexible NWN PDs can enable 250 bending cycles at different bending radii and 1000 bending cycles at fixed bending radii with no performance degradation being observed. These results indicate our welding strategy is very powerful for improving the performance of the NW device with applications in the wearable field.

10.
New Phytol ; 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31917864

RESUMO

The key process of sexual reproduction is the successful fusion of the sperm and egg cell. Distinct from dynamic and flagellated animal sperm cells, higher flowering plant sperm cells are immotile. Therefore, plants have evolved a novel reproductive system to achieve fertilization and generate progenies. Plant sexual reproduction consists of multiple steps mainly including gametophyte development, pollen-pistil recognition, pollen germination, double fertilization and post-fertilization. During reproduction, active production, consumption and recycling of cellular components and energy are critically required to achieve fertilization. However, the underlying machinery of cellular degradation and turnover remains largely unexplored. Autophagy, the major catabolic pathway in eukaryotic cells, participates in regulating multiple aspects of plant activities including abiotic and biotic stress resistance, pathogen response, senescence, nutrient remobilization and plant development. Nevertheless, a key unanswered question is how autophagy regulates plant fertilization and reproduction. Here, we focus on comparing and contrasting autophagy in several key reproductive processes of plant and animal systems to feature important distinctions and highlight future research directions of autophagy in angiosperm reproduction. We further discuss the potential crosstalk between autophagy and programmed cell death that are often considered as two disconnected events in plant sexual reproduction.

11.
Oncol Rep ; 43(2): 491-502, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31894343

RESUMO

MicroRNA­708­5p (miR­708­5p) and epithelial­â€‹to­mesenchymal transition (EMT) have been widely identified to contribute to the pathogenesis and progression of multiple cancers. However, the connection between miR­708­5p and EMT has not been sufficiently clarified. Therefore, our research aimed to investigate the impact of miR­708­5p on EMT and the metastasis of osteosarcoma (OS). We first analyzed the differentially expressed microRNAs (DEmiRNAs) from the GSE70367 dataset. We found that the expression of miR­708­5p was lower in OS cells. Overexpression of miR­708­5p was able to impair the migration and invasion of OS cells. Moreover, miR­708­5p inhibited EMT of OS cells MG63 and SaOS­2, wherein E­cadherin was increased, and N­cadherin, vimentin, and Snail were decreased. Semaphorin 4C (SEMA4C), mitogen­activated protein kinase kinase kinase 3 (MAP3K3), and zinc finger E­box­binding homeobox 1 (ZEB1) were predicted as target genes of miR­708­5p by bioinformatics method. Only ZEB1, one of the EMT­inducing transcription factors, was validated as the direct target gene of miR­708­5p in OS cells through dual­luciferase reporter assay and Western blot analysis. Knockdown of ZEB1 was found to inhibit the metastasis of MG63 and SaOS­2 cells, whereas ZEB1 over-expression promoted their metastasis. In summary, miR­708­5p impaired the metastasis and EMT of OS, which was found to be mediated by inhibition of ZEB1.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31895540

RESUMO

Organic-inorganic hybrid lead halide perovskites have attracted much attention in the photoelectric field due to their excellent characteristics, such as a tunable band gap, simple fabrication process, and high photoelectric conversion efficiency. However, the commercialization of the perovskite-based devices still faces many challenges, one of which is the inclusion of the toxic lead. Herein, we demonstrated a two-step solution method for synthesizing tin-based perovskite nanowires (NWs) with their application in photodetectors (PDs). By changing the halide exchange time and the Sn content in the precursor, the dark current of the CsPbxSn1-x(BryI1-y)3 perovskite NW PDs increased with increasing content of tin and decreased with increasing Br concentration, and the lowest dark current with a value of 0.672 nA at 1 V was achieved for the perovskite alloy NW PDs synthesized with 0.5 mg mL-1 SnI2. Our optimized perovskite alloy NW PDs showed high performance with a linear dynamic range of up to 120 dB, a rising/falling time of 4.25/4.82 ms, and a detectivity of 2 × 1010 Jones. In addition, our Sn-based perovskite NW devices could maintain good performance after storing in air for 30 days. These results demonstrated good practical application for the Sn-based perovskite NW devices.

13.
Zhongguo Zhen Jiu ; 40(1): 89-95, 2020 Jan 12.
Artigo em Chinês | MEDLINE | ID: mdl-31930906

RESUMO

Acupuncture has remarkable effects on treating functional gastrointestinal diseases, but its central mechanism is not clear. At present, the research has mainly focused on several central nuclei, such as the dorsal vagus complex (DVC), nucleus raphe magnus (NRM), locus coeruleus (LC), subnucleus reticularis dorsalis (SRD), hypothalamic paraventricular nucleus (PVN), cerebellar fastigial nucleus (FN), central amygdala (CeA), etc. It is not clear whether the nuclei are involved in acupuncture regulation of gastric function through certain interrelation. A further summary of related literature indicates that many brain regions or nuclei in the central nervous system are closely related to gastric function, such as DVC, NRM, parabrachial nuclei (PBN), LC, periaqueductal gray (PAG), cerebellum, PVN, arcuate nucleus (Arc), hippocampus, CeA, etc. Most of these nuclei have certain fiber connections with each other, in which DVC is the basic center, and other nuclei are directly or indirectly involved in the regulation of gastric function through DVC. Is DVC the key target in acupuncture regulation of gastric function? Does other nuclei have direct or indirect neural circuit with DVC to participate in the regulation of gastric function by acupuncture, such as the possibility of CeA-DVC neural loop in acupuncture regulating gastric function. Therefore, more advanced techniques such as photogenetics, chemical genetics should be introduced and the central mechanism of acupuncture on regulating gastric function with DVC as center, from the view of nerve loop, will become the focus of further research, which could explain the central integration mechanism of acupoint compatibility by modern neuroscience technology.

14.
Neurosci Lett ; 718: 134745, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31923521

RESUMO

BACKGROUND: Most studies assessing brain-personality mechanisms have used Western personality questionnaires. However, Western personality questionnaires may not objectively reflect the personality characteristics of individuals in Eastern cultures such as China. Hence, we adopted the functional magnetic resonance imaging (fMRI) and the Chinese localized scale, FPPI, to explore the brain mechanisms differences of typical yin and typical yang personalities of individuals in China. METHODS: 30 typical yin personality participants (TYI) and 34 typical yang personality participants (TYA) were enrolled according to the FPPI. The group differences of the functional brain networks among 90 specific brain regions were mapped using fMRI data and then analyzed by the conventional network metrics (CNM) and frequency subgraph mining (FSM). RESULTS: The CNM and FSM differences between two typical personality groups were traced to the frontal, temporal, and parietal cortices. The yin group, reflecting the rich emotions and feelings of individuals, showed higher betweenness centrality (BCi) and nodal efficiency (Ei) values in putamen and middle frontal gyrus. The yang group, reflecting active behaviors and tendency to adapting to the changing surroundings, showed higher BCi and Ei values in precuneus, posterior cingulate gyrus, and inferior parietal lobule, brain areas in the default mode network (DMN). CONCLUSION: These results supplied evidence for the neurobiological differences between typical yin and typical yang personality participants based on Chinese culture. These results also provide a new perspective to help researchers understand brain mechanism differences between yin and yang personality groups in the Chinese culture.

15.
Artigo em Inglês | MEDLINE | ID: mdl-31935182

RESUMO

Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.) G. Don, was taxonomically characterized based on a polyphasic investigation. It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species. The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species. The menaquinone was MK-8(H4). The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI) and unidentified lipids (ULs). The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0. The cell wall peptidoglycan contains l-ornithine as diagnostic diamino acid and an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp. The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cerasi sp. nov. is proposed, with CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain. The DNA G+C composition is 72.3 mol%. The availability of new data allows for an emended description of the genus Ornithinimicrobium.

16.
JCI Insight ; 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31895697

RESUMO

Ultrasound-induced microbubble (USMB) cavitation is widely used to promote drug delivery. Our previous study investigated USMB targeting round window membrane by applying the ultrasound transducer to tympanic bulla. In the present study we further extended the use of this technology to enhance drug delivery to inner ear by introducing the ultrasound transducer into external auditory canal (EAC) or applying it to skull. Using a three-dimensional-printed diffusion apparatus mimicking the pathway for ultrasound passing through and reaching middle ear cavity in vitro, both models simulating the transcanal and transcranial approach demonstrated 4.8-fold and 3.7-fold higher delivery efficiencies, respectively. In vivo model of guinea pigs, by filling tympanic bulla with microbubbles and biotin-fluorescein (biotin-FITC), USMB applied transcanally and transcranially induced 2.8-fold and 1.5-fold increases in biotin-FITC delivery efficiencies, respectively. In addition, the gentamicin uptake by cochlear and vestibular hair cells and gentamicin-induced hair cell loss were significantly enhanced following transcanal application of USMB. On the 28th day after transcanal USMB, safety assessment showed no significant changes in the hearing thresholds and the integrity of cochlea. These are the first results to demonstrate the feasibility and support the potential clinical application of applying USMB via EAC to facilitate drug delivery into inner ear.

17.
Cancer Med ; 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31902138

RESUMO

BACKGROUND: Acute myeloid leukemia (AML) is a common hematologic neoplasm with high incidence and mortality in the elderly. Our aims were to explore risk factors for early mortality in elderly AML patients and develop a new prognostic score. METHODS: We enrolled newly diagnosed AML patients age 60 and above at Taipei Veterans General Hospital between July 2008 and May 2017. The primary endpoint was early mortality, defined as death within two months after AML diagnosis. A multivariate Cox proportional hazards model was used to build a risk-scoring system incorporating significant risk factors for AML. RESULTS: The final cohort included 277 elderly AML patients. The median age was 74 (range 60-96), and 61.7% were male. The two-month mortality rate was 29.9%. Age ≥ 80 (adjusted HR 1.88), myocardial infarction (adjusted HR 1.87), ECOG ≥ 2 (adjusted HR 2.10), complex karyotype (adjusted HR 3.21), bone marrow blasts ≥ 70% (adjusted HR 1.88), WBC ≥ 100 × 109 /L (adjusted HR 3.31), and estimated glomerular filtration rate (eGFR) < 45 (adjusted HR 2.60) were identified as independent predictors for early mortality in the multivariate analysis. A simplified score incorporating the seven factors was developed with good predictive ability measured by Harrell's C statistic [0.72 (95% CI 0.66-0.78)]. CONCLUSIONS: We identified seven potential risk factors for early mortality and built up a new prognostic score for elderly AML patients. The new score may help clinicians stratify patients and initiate appropriate management. Further validation of our findings on other cohorts is warranted.

18.
Cell Biol Int ; 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31903696

RESUMO

The role of long non-coding RNAs (lncRNAs) in tumorigenesis and development of ovarian cancer (OC) has caught the attention of scientists. UNC5B antisense RNA 1 (UNC5B-AS1) is a newly identified carcinogenic lncRNA in thyroid papillary carcinoma, but its role in OC remains unclear. This study is proposed to investigate function and mechanism of UNC5B-AS1 in OC. UNC5B-AS1 expression in OC samples was obtained from GEPIA based on TCGA data. Gene expressions were detected by RT-qPCR and western blot. Biological functions of UNC5B-AS1 were assessed by CCK-8, colony formation, and caspase-3 analysis. GEPIA revealed the UNC5B-AS1 upregulation in OC samples. RT-qPCR assay confirmed the upregulation of UNC5B-AS1 in OC cells. Functionally, depletion of UCN5B-AS1 hindered proliferation and prompted apoptosis in OC cells. Mechanistically, we found that UNC5B-AS1 interacted with zeste 2 polycomb repressive complex 2 subunit (EZH2) to trigger trimethylation of histone H3 at lysine 27 (H3K27me3) on N-myc downstream regulated gene-2 (NDRG2) promoter and epigenetically repress NDRG2. Rescue assay indicated the participation of NDRG2 in the regulation of UNC5B-AS1 on OC progression. Together, we firstly illustrated that UNC5B-AS1 promoted ovarian cancer progression by regulating the H3K27me on NDRG2 via EZH2, indicating UNC5B-AS1 as a potential molecular targets for OC treatment. This article is protected by copyright. All rights reserved.

19.
J Chin Med Assoc ; 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31904659

RESUMO

BACKGROUND: Acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) are hematological diseases predominantly occurring in older patients. Although allogeneic hematopoietic stem cell transplantation (allo-HSCT) remains the curative therapy for refractory AML or high-risk MDS, old age is often a hurdle to the procedure. We conducted a retrospective study to analyze the prognostic factors predicting outcomes of older patients undergoing allo-HSCT for acute leukemia and MDS. METHODS: We collected data from patients diagnosed with acute leukemia or MDS, who underwent allo-HSCT at age more than 50 years and reviewed clinical characteristics including age, sex, underlying disease, European Group for Blood and Bone Marrow Transplantation (EBMT) risk score, and presence of acute graft-versus-host disease (aGVHD) or chronic GVHD (cGVHD). The Cox proportional hazard model was adopted to explore the independent prognostic factors for overall survival (OS), progression-free survival (PFS), and non-relapse mortality (NRM). RESULTS: A total of 85 older patients were included, with the median age at allo-HSCT being 55 years. The significant prognostic factors for worse OS or PFS were an EBMT risk score > 3 and grade III-IV aGVHD, while patients with moderate to severe cGVHD would have better OS or PFS. Interestingly, it's not chronic GVHD but grade III-IV aGVHD that significantly correlated with NRM. CONCLUSION: This cohort study suggests that an EBMT risk score > 3 and grade III-IV aGVHD predict poor outcomes, and careful management of GVHD may allow better survival for older patients undergoing allo-HSCT.

20.
J Am Chem Soc ; 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31927989

RESUMO

Grafting-from (GF) is an important yet underdeveloped strategy toward protein-polymer conjugates. Here, we report a simple cryopolymerization method that enables highly efficient GF synthesis of cell-penetrating protein-polydisulfide conjugates. Rapid and controlled ring-opening polymerization of 1,2-dithiolanes under cryo-conditions can be initiated by proteins bearing a reactive cysteine, owing to both favored thermodynamics and augmented kinetics arising from frozen-induced high local concentration of substrates. This method is applicable to various wild-type or genetically engineered proteins without the need of chemical installation of an initiation group. The resulting conjugates can be reversibly degrafted under mild conditions to regenerate functional "native" proteins in a traceless fashion. These unique features make such conjugates highly useful in applications such as a dynamic switch of protein functions, cytosolic delivery of protein therapeutics, and protein purification. The method is also potentially useful for the in situ growth of other types of polymers from protein surface.

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