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1.
J Hazard Mater ; 423(Pt A): 127104, 2021 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-34523482

RESUMO

Soils contamination with Cd result in detriment to the environmental quality. In-situ immobilization methods by applying clay minerals have been gaining prominence. The effects on sepiolite of thermal activation at different temperatures (300-750 °C), for removing Cd from aqueous solutions were evaluated, in order to consider their further application for soil remediation. The influence of activation temperature was investigated using XRD, SEM, and N2 adsorption-desorption measurements. The S-600 exhibited the maximum adsorption capacity (21.28 mg/g), despite its lower SSA, and Langmuir model described the adsorption isotherms better than the Freundlich equation. TCLP was used to quantify the remediation effects of thermal-activated sepiolite on simulated soils artificially polluted with Cd. The results indicated that the mobility of Cd in soil was effectively reduced after treating with thermal-activated sepiolite and the use of S-600 was the most efficient, reducing the TCLP-Cd by approximately 73% compared with the control test. The main remediation mechanism was considered as the cation exchange of Cd by Mg at the edges of octahedral sheet. This study showed that thermal-activated sepiolite could be promising amendments for remediation of Cd-contaminated soil.

2.
EBioMedicine ; 72: 103591, 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34563924

RESUMO

BACKGROUND: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease with high mortality, however with no effective therapy available. METHODS: The effect of favipiravir (FPV) in treating SFTS was evaluated by an integrated analysis on data collected from a single-arm study (n=428), a surveillance study (n=2350) and published data from a randomized controlled trial study (n=145). A 1:1 propensity score matching was performed to include 780 patients: 390 received FPV and 390 received supportive therapy only. Case fatality rates (CFRs), clinical progress, and adverse effects were compared. FINDINGS: FPV treatment had significantly reduced CFR from 20.0% to 9.0% (odds ratio 0.38, 95% confidence interval 0.23-0.65), however showing heterogeneity when patients were grouped by age, onset-to-admission interval, initial viral load and therapy duration. The effect of FPV was significant only among patients aged ≤70 years, with onset-to-admission interval ≤5 days, therapy duration ≥5 days or baseline viral load ≤1 × 106 copies/mL. Age-stratified analysis revealed no benefit in the aging group >70 years, regardless of their sex, onset-to-admission interval, therapy duration or baseline viral load. However, for both ≤60 and 60-70 years groups, therapy duration and baseline viral load differentially affected FPV therapy efficiency. Hyperuricemia and thrombocytopenia, as the major adverse response of FPV usage, were observed in >70 years patients. INTERPRETATION: FPV was safe in treating SFTS patients but showed no benefit for those aged >70 years. Instant FPV therapy could highly benefit SFTS patients aged 60-70 years. FUNDING: China Natural Science Foundation (No. 81825019, 82073617 and 81722041) and China Mega-project for Infectious Diseases (2018ZX10713002 and 2015ZX09102022).

3.
Oncol Lett ; 22(3): 657, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34386079

RESUMO

Melanoma, the most aggressive skin cancer, is mainly treated with BRAF inhibitors or immunotheareapy. However, most patients who initially responded to BRAF inhibitors or immunotheareapy become resistant following relapse. Ferroptosis is a form of regulated cell death characterized by its dependence on iron ions and the accumulation of lipid reactive oxygen species (ROS). Recent studies have demonstrated that ferroptosis is a good method for tumor treatment, and iron homeostasis is closely associated with ferroptosis. Iron regulatory protein (IRP)1 and 2 play important roles in maintaining iron homeostasis, but their functions in ferroptosis have not been investigated. The present study reported that the expression of IRP1 and IRP2 was increased by the ferroptosis inducers erastin and RSL3 in melanoma cells. Depletion of IRP1 significantly suppressed erastin- and RSL3-induced ferroptosis. IRP2 had a weak effect but could enhance the promoting function of IRP1 on ferroptosis. Further, erastin and RSL3 promoted the transition of aconitase 1 to IRP1, which regulated downstream iron metabolism proteins, including transferrin receptor (TFRC), ferroportin (FPN) and ferritin heavy chain 1 (FTH1). Moreover, overexpression of TFRC and knockdown of FPN and FTH1 significantly promoted erastin- and RSL3-induced ferroptosis in IRP1 knockdown melanoma cells. Collectively, the present findings indicate that IRP1 plays an essential role in erastin- and RSL3-induced ferroptosis by regulating iron homeostasis.

4.
J Neurol ; 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-34459986

RESUMO

The literature on cases of acute disseminated encephalomyelitis (ADEM) associated with SARS-CoV-2 infection has been rapidly increasing. However, the specific clinical features of ADEM associated with SARS-CoV-2 (SARS-CoV-2-ADEM) have not been previously evaluated. We screened all articles resulting from a search of PubMed and Web of Science databases looking for reports of ADEM published between December 01, 2019, and June 5, 2021. Of the 48 ADEM cases identified from 37 studies, 34 (71%) had ADEM while 14 (29%) were of AHLE. RT-PCR for SARS-CoV-2 was positive in 83% (n = 19) of patients. 26 patients (54%) were male, and 18 patients (38%) were female, with a male to female sex ratio of 1.4:1; median age was 44 (1.4-71) years. 9 patients (19%, 9/48) were children. Of the 9 children patients, their median age was 9 years (range 1.4-13 years), 6 patients (67%) were female, and 2 patients (22%) were male, with a female to male sex ratio of 3:1.39 patients (81%) was performed CSF analysis. PCR for SARS-CoV-2 tested positive in 3 patients (14%, 3/22) on CSF sample. 31 (64%) of patients had a poor outcome on discharge from hospital. Five (10%) patients died in hospital. Compared to classic ADEM, SARS-CoV-2-ADEM have a more longer duration between the onset of the antecedent infective symptoms and the start of ADEM symptoms, the older age distribution of the patients, relatively poor outcome, a lower full recovery rate, a more frequently brain lesions involved the periventricular white matter and corpus callosum, and less frequently affected the deep gray matter. Taken together, the present comprehensive review reveals that although rare, ADEM can be associated with SARS-CoV-2 infection. SARS-CoV-2-ADEM seems to share most features of classic ADEM, with moderate discrepancies from the classical ADEM.

6.
Front Neurosci ; 15: 647266, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34121985

RESUMO

Background: Aggregation and neurotoxicity of the presynaptic protein α-synuclein and the progressive loss of nigral dopaminergic neurons are believed to be the key hallmarks of Parkinson's disease (PD). A53T mutant α-synuclein causes early onset PD and more severe manifestations. A growing body of evidence shows that misfolding or deposition of α-synuclein is linked to the maintenance of mitochondrial dynamics, which has been proven to play an important role in the pathogenesis of PD. It has been observed that Dl-3-n-butylphthalide (NBP) may be safe and effective in improving the non-tremor-dominant PD. However, the potential mechanism remains unclear. This study aimed to investigate whether NBP could decrease the loss of dopaminergic neurons and α-synuclein deposition and explore its possible neuroprotective mechanisms. Methods: A total of 20 twelve-month-old human A53T α-synuclein transgenic mice and 10 matched adult C57BL/6 mice were included in the study; 10 adult C57BL/6 mice were selected as the control group and administered with saline (0.2 ml daily for 14 days); 20 human A53T α-synuclein transgenic mice were randomly divided into A53T group (treated in the same manner as in the control group) and A53T + NBP group (treated with NBP 0.2 ml daily for 14 days). Several markers of mitochondrial fission and fusion and mitophagy were determined, and the behavioral, olfactory, and cognitive symptoms were assessed as well. Results: In the present study, it was observed that the A53T-α-synuclein PD mice exhibited anxiety-like behavioral disturbance, impairment of coordination ability, memory deficits, and olfactory dysfunction, loss of dopaminergic neurons, and α-synuclein accumulation. Meanwhile, the mitofusin 1 expression was significantly decreased, and the mitochondrial number and dynamin-related protein 1, Parkin, and LC3 levels were increased. The detected levels of all markers were reversed by NBP treatment, and the mitochondrial morphology was partially recovered. Conclusion: In the present study, a valuable neuropharmacological role of NBP has been established in the A53T-α-synuclein PD mouse model. Possible neuroprotective mechanisms might be that NBP is involved in the maintenance of mitochondrial dynamics including mitochondrial fission and fusion and clearance of damaged mitochondria. It is essential to perform further experiments to shed light on the precise mechanisms of NBP on mitochondrial homeostasis.

8.
QJM ; 2021 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-34043803

RESUMO

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been linked to the Guillain-Barré syndrome (GBS). The objective of the present study is to identify specific clinical features of cases of GBS reported in the literature associated with SARS-CoV-2 infection. We searched Pubmed, and included single case reports and case series with full text in English, reporting original data of patients with GBS and a confirmed recent SARS-CoV-2 infection. Clinical data were extracted.We identified 28 articles (22 single case reports and 6 case series), reporting on a total of 44 GBS patients with confirmed SARS-CoV-2 infection. SARS-CoV-2 infection was confirmed through serum RT-PCR in 72.7% of cases. A total of 40 patients (91%) had symptoms compatible with SARS-CoV-2 infection before the onset of the GBS. The median period between the onset of symptoms of SARS-CoV-2 infection and symptoms of the GBS was 11.2 days (range, 2-23). The most common clinical features were: leg weakness (61.4%), leg parethesia (50%), arm weakness (50.4%), arm paresthesia (50.4%), hyporeflexia/areflexia (48%), and ataxia (22.7%). 38.6% (n = 17) were found to have facial paralysis. Among 37 patients in whom nerve-conduction studies and electromyography were performed, of which 14 patients (31.8%) were consistent with the acute inflammatory demyelinating polyneuropathy (AIDP) subtype of the GBS. The present retrospective analysis support the role of the SARS-CoV-2 infection in the development of the GBS, may trigger GBS as para-infectious disease, and lead to SARS-CoV-2-associated GBS.

9.
Signal Transduct Target Ther ; 6(1): 145, 2021 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-33859168

RESUMO

Severe fever with thrombocytopenia syndrome (SFTS) virus (SFTSV) is an emerging tick-borne virus with high fatality and an expanding endemic. Currently, effective anti-SFTSV intervention remains unavailable. Favipiravir (T-705) was recently reported to show in vitro and in animal model antiviral efficacy against SFTSV. Here, we conducted a single-blind, randomized controlled trial to assess the efficacy and safety of T-705 in treating SFTS (Chinese Clinical Trial Registry website, number ChiCTR1900023350). From May to August 2018, laboratory-confirmed SFTS patients were recruited from a designated hospital and randomly assigned to receive oral T-705 in combination with supportive care or supportive care only. Fatal outcome occurred in 9.5% (7/74) of T-705 treated patients and 18.3% (13/71) of controls (odds ratio, 0.466, 95% CI, 0.174-1.247). Cox regression showed a significant reduction in case fatality rate (CFR) with an adjusted hazard ratio of 0.366 (95% CI, 0.142-0.944). Among the low-viral load subgroup (RT-PCR cycle threshold ≥26), T-705 treatment significantly reduced CFR from 11.5 to 1.6% (P = 0.029), while no between-arm difference was observed in the high-viral load subgroup (RT-PCR cycle threshold <26). The T-705-treated group showed shorter viral clearance, lower incidence of hemorrhagic signs, and faster recovery of laboratory abnormities compared with the controls. The in vitro and animal experiments demonstrated that the antiviral efficacies of T-705 were proportionally induced by SFTSV mutation rates, particularly from two transition mutation types. The mutation analyses on T-705-treated serum samples disclosed a partially consistent mutagenesis pattern as those of the in vitro or animal experiments in reducing the SFTSV viral loads, further supporting the anti-SFTSV effect of T-705, especially for the low-viral loads.

10.
Biomed Mater ; 16(1): 015001, 2020 11 27.
Artigo em Inglês | MEDLINE | ID: mdl-33245056

RESUMO

It has been established that scar acellular matrices (AMs), which allow cell proliferation, have similar characteristics. The aim of this study was to investigate the repair effect of scar AMs on animals, thus providing a reference for clinical application. Selected mature and immature scar AMs were implanted into animals, and then a negative control group was set for comparison. The effect of scar AMs on wound healing was observed through tissue staining, RT-qPCR, and immunohistochemistry. The materials showed milder inflammation and faster extracellular matrix (ECM) deposition than the negative control group. The ECM deposition and new vessels increased over time. However, the arrangement of ECM in mature scar AM was more regular than in immature scar AM and the negative control group, and more new vessels grew in the mature scar AM group than in the immature scar AM group and negative control group over the same period. The transforming growth factor-ß level was elevated at one month, two months, and six months. COLA1 and vimentin levels all peaked at six months. Matrix metalloproteinase and TIMP1 were also elevated at different months. Collectively, scar AMs can effectively promote wound healing and vascularization. Mature scar AMs have a better regeneration effect.

11.
Comp Biochem Physiol B Biochem Mol Biol ; 243-244: 110429, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32097715

RESUMO

Growth hormone (ScGH) and growth hormone receptor (ScGHR) genes from the barbel chub (Squaliobarbus curriculus), in addition to their cDNAs, were cloned. The associations between their mRNA expression levels and growth-related traits were analysed, and the differences in the levels of expression of growth regulation-related genes between the largest and smallest individuals were compared. The full-length 1182-bp cDNA of ScGH contained a 633-bp open reading frame (ORF), and the length of the gene had 2492 bp. The full-length 2825-bp cDNA of ScGHRa contained a 1818-bp ORF, and the gene had 6970 bp. The full-length 2822-bp cDNA of ScGHRb contained a 1737-bp ORF, and the gene had 8149 bp. Quantitative real-time PCR revealed that ScGH was only expressed in the pituitary. ScGHRa was expressed predominantly in muscle, and the expression level of ScGHRb was the highest in the liver. The ScGHRa mRNA levels in the muscle were significantly negatively correlated with the caudal peduncle length. However, no correlation between growth-related traits and ScGH and ScGHRb expression levels were found. Pituitary ScGH, liver GHRb and liver insulin-like growth factor I (igf-1) expression levels were significantly higher in the largest individuals than those in the smallest S. curriculus individuals. Contrarily, the largest individuals had significantly lower expression levels of muscle igf-1 and liver myog than the smallest individuals. Overall, our results provide novel molecular information for growth-regulation study of S. curriculus.


Assuntos
Cyprinidae/genética , Proteínas de Peixes/genética , Regulação da Expressão Gênica/genética , Hormônio do Crescimento/genética , Receptores da Somatotropina/genética , Sequência de Aminoácidos , Animais , Clonagem Molecular , Cyprinidae/crescimento & desenvolvimento , Cyprinidae/metabolismo , DNA Complementar/genética , Proteínas de Peixes/metabolismo , Hormônio do Crescimento/química , Hormônio do Crescimento/metabolismo , Fases de Leitura Aberta , Especificidade de Órgãos , Filogenia , RNA Mensageiro/metabolismo , Receptores da Somatotropina/química , Receptores da Somatotropina/metabolismo
13.
Exp Ther Med ; 18(5): 3837-3844, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31616511

RESUMO

Brain-derived neurotrophic factor (BDNF) is a growth factor crucial for neuronal survival, while its role in subarachnoid hemorrhage (SAH)-induced neuronal apoptosis remains unclear. The aim of the present study was to investigate whether administering exogenous BDNF can protect against neuronal apoptosis and neurological deficits following SAH in a rat model. The BDNF level was found to be significantly decreased in the basal cortex at 6, 12, 24, 48 and 72 h following SAH. Exogenous BDNF significantly decreased the expression of Bax and reduced activation of caspase-3 and caspase-9 and the number of apoptotic neurons. Moreover, exogenous BDNF treatment significantly improved the neurological deficits at 72 h and long-term behavioral deficits (day 14) following SAH in a rat model. These findings indicate that exogenous BDNF attenuated SAH-induced neuronal injury in rats.

14.
Mol Carcinog ; 58(11): 2149-2160, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31448838

RESUMO

Autophagy is a self-proteolytic process that degrades intracellular material to maintain cellular homeostasis. Transcription factor EB (TFEB) is the master activator that regulates the transcription of genes involved in autophagy and lysosomal biogenesis. However, the cotranscriptional factors of TFEB are rarely identified. Here, we found that Yin Yang 1 (YY1) regulated autophagy and lysosome biogenesis in melanoma cells. YY1 cooperates with TFEB to regulate autophagy through controlling the transcription of autophagy and lysosome biogenesis related genes. Moreover, suppression of YY1 enhanced the antitumor efficiency of vemurafenib both in vitro and in vivo. Collectively, these studies identify YY1 as a novel cotranscription factor of TFEB in regulating autophagy and lysosomal functions and suggest YY1 could be a therapeutic target in cancer treatment.


Assuntos
Autofagia/genética , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/genética , Melanoma/genética , Fator de Transcrição YY1/genética , Animais , Regulação Neoplásica da Expressão Gênica , Células HEK293 , Xenoenxertos , Humanos , Lisossomos/genética , Melanoma/patologia , Camundongos , Plasmídeos/genética
15.
Sensors (Basel) ; 19(12)2019 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-31212964

RESUMO

The European Space Agency (ESA) Climate Change Initiative (CCI) project combines multi-sensors at different microwave frequencies to derive three harmonized soil moisture products using active, passive and combined approaches. These long-term soil moisture products assist in understanding the global water and carbon cycles. However, extensive validations are a prerequisite before applying the retrieved soil moisture into climatic or hydrological models. To fulfill this objective, we assess the performances of three CCI soil moisture products (active, passive and combined) with respect to in-situ soil moisture networks located in China, Spain and Canada. In order to compensate the scale differences between ground stations and the CCI product's coarse resolution, we adopted two upscaling approaches of Inverse Distance Weighting (IDW) interpolation and simple Arithmetic Mean (AM). The temporal agreements between the satellite retrieved and ground-measured soil moisture were quantified using the unbiased root mean square error (ubRMSE), RMSE, correlation coefficients (R) and bias. Furthermore, the temporal variability of the CCI soil moisture is interpreted and verified with respect to the Tropical Rainfall Measuring Mission (TRMM) precipitation observations. The results show that the temporal variations of CCI soil moisture agreed with the in-situ ground measurements and the precipitation observations over the China and Spain test sites. In contrast, a significant overestimation was observed over the Canada test sites, which may be due to the strong heterogeneity in soil and vegetation characteristics in accordance with the reported poor performance of soil moisture retrieval there. However, despite a retrieval bias, the relatively temporal variation of the CCI soil moisture also followed the ground measurements. For all the three test sites, the soil moisture retrieved from the combined approach outperformed the active-only and passive-only methods, with ubRMSE of 0.034, 0.050, and 0.050-0.054 m3/m3 over the test sites in China, Spain and Canada, respectively. Thus, the CCI combined soil moisture product is suggested to drive the climatic and hydrological studies.

16.
J Biomater Sci Polym Ed ; 30(9): 769-784, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30950313

RESUMO

INTRODUCTION: Acellular dermal matrix (ADM) is commonly used to treat burn injuries and wounds. In this study, we aimed to analyze the structural and biomechanical characteristics of an ADM from human scars. METHODS: We randomly selected human mature scars, human proliferative scars, and human normal skin as experimental specimens. Split-thickness dermal grafts were obtained using a free-hand graft knife. Samples were subjected to cell lysis to obtain ADMs. Structural analysis was performed via light microscopy, fluorescence microplate reader and scanning electron microscopy. Thereafter, human epithelial stem cells were incubated on these ADMs. Finally, the biomechanical characteristics of the ADMs were analyzed using a tensile machine. RESULTS: Normal skin ADM fibers were arranged in order, whereas proliferative scar ADM fibers were dense and disordered, and mature scar ADM fibers were porous and slightly disordered. The residual DNA of three ADM meet the residual DNA standard of biological material. After incubating human epithelial stem cells on ADMs, cells grew in an aggregated state in both normal skin ADMs and mature scar ADMs; however, cells adhered only on the surface of proliferative scar ADMs. No significant differences were observed in the Young's modulus, relaxation slope, creep slope, creep, or maximum tensile stress among the three ADMs, although significant differences in stress-strain elongation and relaxation were noted. CONCLUSIONS: Our findings showed that mature scar ADMs were similar to proliferative scar ADMs, showing a slight lag compared with normal skin ADMs, providing insights into the biomechanical features of these scar tissues. Abbreviations ADM Acellular dermal matrix H&E Hematoxylin and eosin PBS Phosphate-buffered saline SEM Scanning electron microscopy.


Assuntos
Cicatriz/patologia , Fenômenos Mecânicos , Pele/patologia , Fenômenos Biomecânicos , Humanos
17.
Brain Res ; 1718: 53-63, 2019 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-31026459

RESUMO

Hypertrophic olivary degeneration (HOD) is a rare form of trans-synaptic degeneration characterized by hypertrophy of the inferior olivary nucleus situated in the olivary body, part of the medulla oblongata, representing a major source of input to the cerebellum. HOD typically results from focal lesions interrupting connections from the inferior olive within the dentato-rubro-olivary pathway (DROP), a region also known as the Guillain-Mollaret triangle (GMT) (red nucleus, inferior olivary nucleus, and contralateral dentate nucleus). Clinically, HOD presents classically as palatal tremor and can include dentatorubral tremor and/or ocular myoclonus. Oppenheim first described the enlargement of the ION in his post-mortem study. Since then, a limited number of cases have been reported in the literatures. Thus, we intended to describe the definition, pathophysiology, clinical features, radiological features, diagnosis, and current management of HOD. We provide a comprehensive review of HOD focusing on etiology. The present review may lead to a better understanding of HOD clinical characteristic with the goal of contributing to existing knowledge of this rare disease.


Assuntos
Degeneração Neural/etiologia , Núcleo Olivar/metabolismo , Núcleo Olivar/patologia , Núcleos Cerebelares/patologia , Cerebelo/patologia , Feminino , Humanos , Hipertrofia/etiologia , Hipertrofia/patologia , Imageamento por Ressonância Magnética/métodos , Masculino , Bulbo/patologia , Degeneração Neural/patologia , Núcleo Rubro/patologia , Tremor/patologia
18.
Fish Shellfish Immunol ; 86: 794-804, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30557607

RESUMO

The grass carp reovirus (GCRV) has been shown to cause lethal infections in the grass carp Ctenopharyngodon idella (C. idella). In order to investigate the immune response to GCRV infection, the full-length cDNA sequences of coagulation factor VIII (CiFVIII) and plasminogen (CiPLG) from C. idella were cloned and their involvement in the immune response was studied. The immunity factor levels in C. idella with different GCRV resistances were also analyzed. The full-length 2478 bp cDNA of CiFVIII contained an open reading frame of 1965 bp and encoded a putative polypeptide of 654 amino acid residues. The full-length 2907 bp cDNA of CiPLG contained an open reading frame of 2133 bp and encoded a putative polypeptide of 710 amino acid residues. CiFVIII was closely clustered with that of Clupea harengus. CiPLG was first clustered with those of Cyprinus carpio and Danio rerio. CiFVIII transcripts were most abundant in the liver and least in the skin. The highest expression level of CiPLG was observed in liver and the lowest in muscle. Expression levels of CiFVIII in gill, head kidney and spleen, and expression levels of CiPLG in gill, intestine and liver all reached the maximum at 72 h post GCRV infection. In spleen, expression levels of CiFVIII and CiPLG were significantly positively correlated. The activities of T-AOC, LSZ and IgM in R♂ were significantly higher than those in O♂. Likewise, T-AOC and LSZ activities in F1 were significantly higher than f1 individuals (P < 0.01). These results indicated that CiFVIII and CiPLG may play important roles in the immune response to GCRV infection. In addition, antioxidant ability and serum immune factor activity may confer a different viral resistance to C. idella.


Assuntos
Carpas/genética , Carpas/imunologia , Doenças dos Peixes/imunologia , Proteínas de Peixes/genética , Proteínas de Peixes/imunologia , Regulação da Expressão Gênica/imunologia , Imunidade Inata/genética , Sequência de Aminoácidos , Animais , Clonagem Molecular , Fator VIII/química , Fator VIII/genética , Fator VIII/imunologia , Proteínas de Peixes/química , Perfilação da Expressão Gênica/veterinária , Filogenia , Plasminogênio/química , Plasminogênio/genética , Plasminogênio/imunologia , Reoviridae/fisiologia , Infecções por Reoviridae/imunologia , Alinhamento de Sequência/veterinária
19.
Fish Shellfish Immunol ; 83: 292-307, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30218823

RESUMO

The barbel chub (Squaliobarbus curriculus) is a kind of small size commercial fish species that is widely spread in Asia and has shown significant resistance to disease. In this study, the full-length cDNA sequences of Toll-like receptor (TLR) 7 and 8 from S. curriculus, designated as ScTLR7 and ScTLR8, were cloned, and their differences in the structure and the responses to the grass carp (GCRV) infection and lipopolysaccharide stimulation were investigated. The full-length 3715 base pair (bp) cDNA of ScTLR7 contained a complete open reading frame of 3162 bp and encoded a putative polypeptide of 1053 amino acid residues. The full-length 4624 base pair (bp) cDNA of ScTLR8 contained a complete open reading frame of 3072 bp and encoded a putative polypeptide of 1023 amino acid residues. ScTLR7 and ScTLR8 consisted of N-terminal signal peptide, leucine-rich repeats (LRRs), and Toll/IL-1 Receptors domain. LRR motifs of ScTLR7 and ScTLR8 bend into horseshoe-like solenoid structure, while the number of LRRs between the two genes is different. Phylogenetic analysis showed that both the ScTLR7 and ScTLR8 were closely clustered with Ctenopharyngodon idellus and Megalobrama amblycephala. Quantitative real-time polymerase chain reaction analysis showed that the expression levels of ScTLR7 in S. curriculus were most abundant in the brain followed by the spleen and heart, and the lowest in the intestine. The highest expression level of ScTLR8 was observed in spleen and the lowest in the liver. After LPS stimulation, the relative expression levels of both ScTLR7 and ScTLR8 exhibited an overall trend of up-regulation. The expression levels of type I-IFN showed an overall trend of down-regulation at time points of 12, 24, 72 and 168 h compared to that of 6 h after LPS stimulation. Compared to 6 h post GCRV infection, the transcription level of ScTLR7 was up-regulated from 12 to 168 h, and transcription levels of ScTLR8, MyD88, and type I-IFN were firstly up-regulated from 12 to 72 h, and then down-regulated from 72 to 168 h. Correlation analysis showed that expression level of ScTLR7 in the spleen was significantly positively correlated with that of MyD88 (Pearson correlation coefficient: 0.909, P: 0.033), and a significantly positive correlation was also observed between expression levels of MyD88 and type I IFN (Pearson correlation coefficient: 0.962, P: 0.009), after GCRV stimulation. These results indicate that ScTLR7 and ScTLR8 may play important roles in the responses to the grass carp (GCRV) infection and lipopolysaccharide stimulation and trigger different downstream immune signal pathways.


Assuntos
Cyprinidae/imunologia , Proteínas de Peixes/imunologia , Receptor 7 Toll-Like/imunologia , Receptor 8 Toll-Like/imunologia , Animais , Cyprinidae/genética , Doenças dos Peixes/imunologia , Proteínas de Peixes/genética , Lipopolissacarídeos/farmacologia , Reoviridae , Infecções por Reoviridae/imunologia , Infecções por Reoviridae/veterinária , Receptor 7 Toll-Like/genética , Receptor 8 Toll-Like/genética
20.
J Invertebr Pathol ; 156: 1-5, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29894727

RESUMO

Ascospheara apis is a widespread fungal pathogen that exclusively invades honeybee larvae. Thus far, non-coding RNA in A. apis has not yet been documented. In this study, we sequenced A. apis using strand specific cDNA library construction and Illumina RNA sequencing methods, and identified 379 lncRNAs, including antisense lncRNAs, lincRNAs, intronic lncRNAs and sense lncRNAs. Additionally, these lncRNAs were found to be shorter in length and have fewer exons and transcript isoforms than protein-coding genes, similar to those identified in mammals and plants. Furthermore, the existence of 15 predicted lncRNAs of A. apis was confirmed using RT-PCR and expression levels of 11 were lower than those of adjacent protein-coding genes. Our findings not only enlarge the lncRNA database for fungi, but also lay a foundation for further investigation of potential lncRNA-mediated regulation of genes in A. apis.


Assuntos
Fungos/genética , RNA Fúngico/genética , RNA Longo não Codificante/genética , Animais , Abelhas/parasitologia , RNA Fúngico/análise , RNA Longo não Codificante/análise
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