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1.
Talanta ; 266(Pt 1): 124955, 2024 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-37478763

RESUMO

Herein, a self on electrochemiluminescence (ECL) biosensor was constructed by pH stimuli response controlled release strategy, in which SiO2-PEI as the carrier, BSA/luminol-Ab2 as the encapsulated substance, gold nanoparticles (Au NPs) as the blocking cap, glucose as the inducer. In addition, CeO2-Au was used as catalyst, which generated more O2•- to increase the ECL signal. Under the action of voltage, the glucose was oxidized to gluconic acid, which induced the pH to decrease accordingly. Therefore, Au NPs were stimulated to fall from the surface of SiO2-PEI, releasing the BSA/luminol-Ab2 to realize self on mode. With such design, the constructed self on ECL biosensor owned an ultrasensitive detection capacity of CYFRA 21-1, showing an excellent linear relationship in the range of 0.001-100000 ng/L and 0.4 fg/mL low limit of detection (LOD). It provided an innovative idea for the biosensor construction to clinical detection of lung cancer.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Luminol/química , Nanopartículas Metálicas/química , Ouro/química , Glucose , Dióxido de Silício , Preparações de Ação Retardada , Técnicas Eletroquímicas , Medições Luminescentes , Limite de Detecção , Concentração de Íons de Hidrogênio
2.
Talanta ; 266(Pt 1): 124961, 2024 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-37480821

RESUMO

Here we put forward an innovative "one master and two servants" strategy for enhancing the ECL performance. A novel ECL luminophore named Zr-TCPP/NH2-BDC (TCPP@UiO-66-NH2) was synthesized by self-assembly of meso-tetra(4-carboxyphenyl)porphine (TCPP) and 4-aminobenzoic acid (NH2-BDC) with Zr clusters. TCPP@UiO-66-NH2 has a porous structure and a highly ordered structure, which allows the molecular motion of TCPP to be effectively confined, thereby inhibiting nonradiative energy transfer. Importantly, TCPP@UiO-66-NH2 has a higher and more stable ECL signal. To further improve the sensitivity of the sensor, we use polydopamine-coated manganese dioxide (PDA@MnO2), which has a double quenching effect, as the quencher. The nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2-N) is one of the ideal markers for the early diagnosis of COVID-19, and its sensitivity detection is of great significance for the prevention and treatment of COVID-19. Thus, we constructed a quenching-type ECL sensor for the ultrasensitive detection of the SARS-CoV-2-N. Its linear range is 10 fg/mL∼1 µg/mL and the calculated detection limit is 1.4 fg/mL (S/N = 3). The spiked recoveries are 97.40-103.8%, with the relative standard deviations (RSD) under 3.0%. More importantly, the technique offers a viable way to identify and diagnose viral infections early.


Assuntos
COVID-19 , Humanos , COVID-19/diagnóstico , Ligantes , Compostos de Manganês , Óxidos , SARS-CoV-2
3.
J Hazard Mater ; 464: 133015, 2024 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-37988942

RESUMO

In the face of diversified analytes, it is a great challenge and infeasible task to design and synthesize corresponding macrocyclic hosts to realize the ideal supramolecular sensing. Herein, we proposed a novel supramolecular sensing strategy, guest adaptative assay (GAA), in which analyte was quantitatively transformed under mild conditions to perfectly adapt to macrocyclic host. As a health-threatening "landmine" in cereals, aflatoxins were converted by the aid of alkali hydrolysis to satisfactorily obtain aflatoxins transformants in ionic state, resulting in sensitive response by the guanidinocalix[5]arene•fluorescein reporter pair. Surprisingly, the established strategy not only exhibited effective practicality in screening out high-risk cereals contaminated with aflatoxins, but also relieved the laborious task of macrocycle design and screening in supramolecular sensing.

4.
J Colloid Interface Sci ; 653(Pt B): 1348-1357, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-37801845

RESUMO

Nitrogen-coordinated manganese atoms on carbon materials denoted as MnNC, serve as the highly active non-precious metal electrocatalysts for oxygen reduction reaction (ORR) in zinc-air batteries (ZABs). Nonetheless, a significant challenge arises from the tendency of Mn atoms to aggregate during heat treatment, thereby compromising ORR performance in ZABs. In this work, the molecular assembly strategy based on the hydrogen bond interaction was employed to fabricate the MnNC electrocatalyst. This approach promotes the dispersion of Mn atoms, creating abundant Mn-Nx active sites. Furthermore, the resulting three-dimensional porous nanostructure, formed by molecular assembly, significantly enhances accessibility to the Mn-Nx active sites. The porous nanostructure not only shortens the diffusion path of reactants and charges but also improves mass transfer. The MnNC exhibits impressive ORR catalytic performance with a half-wave potential of 0.90 V (vs. RHE). The liquid-type ZAB based on MnNC displays a high specific capacity of 816.6 mAh/g and an extended charge-discharge cycle life of 1000 h. Quasi-solid-state ZAB based on MnNC can operate stably for 24 h. This work presents an effective strategy to synthesize transition metal-nitrogen-carbon (MNC) electrocatalysts tailored for long-life zinc-air battery.

5.
Neural Regen Res ; 19(5): 1126-1133, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-37862218

RESUMO

Traumatic spinal cord injury is potentially catastrophic and can lead to permanent disability or even death. China has the largest population of patients with traumatic spinal cord injury. Previous studies of traumatic spinal cord injury in China have mostly been regional in scope; national-level studies have been rare. To the best of our knowledge, no national-level study of treatment status and economic burden has been performed. This retrospective study aimed to examine the epidemiological and clinical features, treatment status, and economic burden of traumatic spinal cord injury in China at the national level. We included 13,465 traumatic spinal cord injury patients who were injured between January 2013 and December 2018 and treated in 30 hospitals in 11 provinces/municipalities representing all geographical divisions of China. Patient epidemiological and clinical features, treatment status, and total and daily costs were recorded. Trends in the percentage of traumatic spinal cord injuries among all hospitalized patients and among patients hospitalized in the orthopedic department and cost of care were assessed by annual percentage change using the Joinpoint Regression Program. The percentage of traumatic spinal cord injuries among all hospitalized patients and among patients hospitalized in the orthopedic department did not significantly change overall (annual percentage change, -0.5% and 2.1%, respectively). A total of 10,053 (74.7%) patients underwent surgery. Only 2.8% of patients who underwent surgery did so within 24 hours of injury. A total of 2005 (14.9%) patients were treated with high-dose (≥ 500 mg) methylprednisolone sodium succinate/methylprednisolone (MPSS/MP); 615 (4.6%) received it within 8 hours. The total cost for acute traumatic spinal cord injury decreased over the study period (-4.7%), while daily cost did not significantly change (1.0% increase). Our findings indicate that public health initiatives should aim at improving hospitals' ability to complete early surgery within 24 hours, which is associated with improved sensorimotor recovery, increasing the awareness rate of clinical guidelines related to high-dose MPSS/MP to reduce the use of the treatment with insufficient evidence.

6.
J Ethnopharmacol ; 319(Pt 2): 117270, 2024 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-37832810

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Huaier (Trametes robiniophila Murr), a traditional Chinese medicinal fungus, possesses potent anticancer efficacy and has been used as an adjuvant medication for liver, breast, gastric, intestinal, and non-small cell lung cancer (NSCLC). However, the potential regulatory functions and underlying molecular mechanisms of Huaier in cisplatin resistance of NSCLC remain unknown. AIM: To evaluate the potential regulatory functions and underlying molecular mechanisms of Huaier in cisplatin resistance of NSCLC. MATERIALS AND METHODS: In vitro and in vivo experiments were employed to evaluate the regulatory functions of Huaier in cisplatin-resistant NSCLC cells. Transcriptome sequencing and validation analyses was undertaken to identify the downstream targets of Huaier. Network pharmacology, ultra-performance liquid chromatography-mass spectroscopy, and in vitro and in vivo experiments were performed to identify key small molecule drug candidates in Huaier and the regulatory mechanisms these employ to suppress cisplatin resistance in NSCLC. RESULTS: Huaier suppressed cisplatin resistance and cancer cell stemness in cisplatin-resistant NSCLC cells, both in vitro and in vivo. Mechanistically, Huaier could suppress expression of interleuken-8 (IL-8) through inhibition of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and activator protein-1 (AP-1), two key transcription factors responsible for the activation of IL-8 transcription. Kaempferol was identified as one of the key small molecule compounds in Huaier that could suppress cisplatin resistance by inhibiting the phosphorylation and nuclear translocation of proto-oncogene c-Jun (JUN) by binding and inhibiting the kinase activity of c-Jun N-terminal protein kinase (JNK). CONCLUSIONS: Huaier suppressed cisplatin resistance of NSCLC cells by inhibiting the JNK/JUN/IL-8 signaling pathway.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Humanos , Cisplatino/farmacologia , Cisplatino/uso terapêutico , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Trametes/química , Trametes/metabolismo , Fator de Transcrição AP-1/metabolismo , Interleucina-8 , Transdução de Sinais , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/metabolismo , Linhagem Celular Tumoral
7.
CNS Neurol Disord Drug Targets ; 23(1): 88-101, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-36573059

RESUMO

The pathophysiological importance of T helper 1 (Th1) and Th2 cell cytokines in pathological pain has been highly debated in recent decades. However, the analgesic strategy targeting individual cytokines still has a long way to go for clinical application. In this review, we focus on the contributions of Th1 cytokines (TNF-α, IFN-γ, and IL-2) and Th2 cytokines (IL-4, IL-5, IL-10, and IL-13) in rodent pain models and human pain-related diseases. A large number of studies have shown that Th1 and Th2 cytokines have opposing effects on pain modulation. The imbalance of Th1 and Th2 cytokines might determine the final effect of pain generation or inhibition. However, increasing evidence indicates that targeting the individual cytokine is not sufficient for the treatment of pathological pain. It is practical to suggest a promising therapeutic strategy against the combined effects of Th1 and Th2 cytokines. We summarize the current advances in stem cell therapy for pain-related diseases. Preclinical and clinical studies show that stem cells inhibit proinflammatory cytokines and release enormous Th2 cytokines that exhibit a strong analgesic effect. Therefore, a shift of the imbalance of Th1 and Th2 cytokines induced by stem cells will provide a novel therapeutic strategy against intractable pain. It is extremely important to reveal the cellular and molecular mechanisms of stem cell-mediated analgesia. The efficiency and safety of stem cell therapy should be carefully evaluated in animal models and patients with pathological pain.

8.
Talanta ; 267: 125160, 2024 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-37678001

RESUMO

A novel competitive ECL immunosensor for detection of 17ß-Estradiol (E2) has been fabricated successfully. CdSe-ZnSe nanocomposites (CdSe-ZnSe NCs) with high catalytic properties, large surface area and good conductivity were used synergistically as the ECL nanocarriers of Pt nanoparticles (PtNPs). The ECL intensity of CdSe-ZnSe NCs increased and stabilized with luminol-PtNPs (luminol-PtNPs@CdSe-ZnSe NCs) because of electron transfer. To achieve the successful assembling of competitive ultrasensitive ECL immunosensor with high sensitivity and synergistic effect, Ag@TiO2 core-shell was introduced as label. Ag@TiO2 acted as a signal amplifier and also exhibited the high catalytic activity towards H2O2. This firmly anchored the E2 Antigen with covalent bond and converted the longer wavelength radiations to shorter wavelength. Under optimized conditions, our proposed strategy quantify the selective and reliable analysis of E2 with detection limit of 2.51 fg/mL (S/N = 3) within the linear range of 0.0001-30 ng/mL. The assembled synergistic strategy-based ECL immunosensor manifested the promising sensitivity, selectability along with high level of repeatability. Thus, the fabricated ECL immunosensor has potential valuable application for E2 detection along with many other environmental pollutants.


Assuntos
Técnicas Biossensoriais , Compostos de Cádmio , Nanopartículas Metálicas , Nanocompostos , Compostos de Selênio , Luminol/química , Compostos de Cádmio/química , Peróxido de Hidrogênio , Imunoensaio , Nanocompostos/química , Estradiol , Medições Luminescentes , Limite de Detecção , Técnicas Eletroquímicas , Nanopartículas Metálicas/química , Ouro/química
9.
Spectrochim Acta A Mol Biomol Spectrosc ; 305: 123460, 2024 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-37778177

RESUMO

In this paper, a novel nitrogen and sulfur co-doped carbon quantum dots (NS-CQDs) were successfully prepared by a dehydration exothermic carbonization method. The NS-CQDs exhibited uniform size distribution, splendid photostability, and bright fluorescence emission with a fluorescence quantum yield of 24.1 %. It was found that Cu2+ could quench the fluorescence at 467 nm based on the static quenching effect when Cu2+ was added to the NS-CQDs. At this time, the fluorescence sensor changed from the "ON" state to the "OFF" state. When glutathione (GSH) was further introduced into the NS-CQDs/Cu2+ system, the fluorescence intensity of NS-CQDs was amazingly restored through the coordination reaction between GSH and Cu2+. The fluorescence sensor changed from the "OFF" state to the "ON" state. Therefore, NS-CQDs as an "ON-OFF-ON" fluorescence sensor was designed for sequential detection of Cu2+ and GSH. Furthermore, this study successfully demonstrated the sensor's ability to selectively detect Cu2+ and GSH within a wide concentration range. Specifically, the detection range for Cu2+ was 0.1 µM-200.0 µM with a detection limit of 0.07 µM, while the range for GSH was 0.6 µM-180.0 µM with a detection limit of 0.1 µM. Most importantly, the NS-CQDs nanosensor could reliably monitor Cu2+ and GSH levels in human serum samples, with significant potential for practical applications.


Assuntos
Carbono , Pontos Quânticos , Humanos , Limite de Detecção , Corantes Fluorescentes , Glutationa , Nitrogênio
10.
J Colloid Interface Sci ; 653(Pt A): 730-740, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-37742432

RESUMO

Transition metal oxides / hydroxides, which have the advantages of wide distribution, low price, low toxicity, and stable chemical properties, have attracted much attention from researchers. Therefore, this work reports the construction of the unique CoMn2O4 nanocages assisted by the Kirkendall effect, and worm-like Ni(OH)2 nanoparticles were grown on the surface via hydrothermal method, the final product CoMn2O4@Ni(OH)2 nanocages were applied to construct efficient and sensitive non-enzymatic glucose electrochemical sensing. The stable three-dimensional hollow CoMn2O4 nanocages structure, not only can provide a wider specific surface area and more abundant active sites, its porous structure also can effectively inhibit the aggregation of nanoparticles, increase the ion diffusion path, shorten the electron transport distance, and improve the electrical conductivity. Loading Ni(OH)2 nanoparticles on the CoMn2O4 nanocages can increase catalytic sites, and further strengthen the electrocatalytic performance. Due to the good synergistic effect between CoMn2O4 and Ni(OH)2, CoMn2O4@Ni(OH)2 nanocages electrochemical sensor can achieve sensitive and rapid detection of trace glucose, with excellent linear range (8.5-1830.5 µM), low limit of detection (0.264 µM), high sensitivity of 0.00646 µA mM-1 cm-2, and outstanding repeatability. More importantly, the sensor has been successfully applied to the determination of blood glucose in human serum with good recoveries (95.64-104.3 %). This work provides a novel scheme for blood glucose detection and expands the application of transition metal oxides / hydroxides in the field of electrochemical sensing.

11.
Genes Dis ; 11(2): 861-873, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-37692502

RESUMO

Microrchidia CW-type zinc finger 2 (MORC2) is a member of the MORC superfamily of nuclear proteins. Growing evidence has shown that MORC2 not only participates in gene transcription and chromatin remodeling but also plays a key in human disease and tumor development by regulating the expression of downstream oncogenes or tumor suppressors. The present review provides an updated overview of MORC2 in the aspect of cancer hallmark and therapeutic resistance and summarizes its upstream regulators and downstream target genes. This systematic review may provide a favorable theoretical basis for emerging players of MORC2 in tumor development and new insight into the potential clinical application of basic science discoveries in the future.

12.
BMC Public Health ; 23(1): 2378, 2023 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-38037037

RESUMO

BACKGROUND: Evidence on the role of physical activity (PA) on healthcare utilization and expenditure is limited in China. We aimed to examine the association between the total physical activity (TPA) per week, healthcare service use and expenditure. METHODS: We extracted the data from China Health and Retirement Longitudinal Study (CHARLS) 2011, 2013, and 2015. Participants more than 50 years old who completed the follow-up for the three waves were enrolled. We converted the volume of vigorous physical activity (VPA) into an equivalent volume of moderate physical activity (MPA) and calculated the TPA per week for each participant. 12,927 of the 17,708 participants in CHARLS were included in our analysis. More than one-third of participants over 50 years old never participate in any moderate or intensity activity, and the median of self-reported moderate or intensity PA was about 525 (IQR 0-1680) MET-minutes per week in 2015. RESULTS: Compared to inactive subjects, the highest level of TPA was significantly related to the decreased risk number of inpatient visits (IRR: 0.58; 95% CI:0.50-0.67, p < 0.001), inpatient hospital days (IRR: 0.60; 95% CI: 0.42-0.84, p < 0.01), healthcare expenditure (IRR: 0.71; 95% CI: 0.65-0.79, p < 0.001) and catastrophic health expenditures (OR: 0.57; 95% CI: 0.45-0.72, p < 0.001) after adjusting for covariates. CONCLUSIONS: Engaging in moderate-to-vigorous PA may drive a potential decrease in healthcare utilization, healthcare expenditure and household financial risk with a dose-response relationship in China, and some possible policy implications in public health may be considered to promote exercise in the middle-aged and elderly to reduce the medical burden on individuals and healthcare systems.


Assuntos
Gastos em Saúde , Aceitação pelo Paciente de Cuidados de Saúde , Idoso , Pessoa de Meia-Idade , Humanos , Estudos Longitudinais , Aposentadoria , Exercício Físico , China
13.
PeerJ ; 11: e16352, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-38025709

RESUMO

Background: Inflammatory and hemostatic disorders in diabetic microangiopathy (DMA) can be linked to thrombin-activatable fibrinolysis inhibitor (TAFI) and its own gene polymorphisms. Thus, the study aimed to investigate the associations of plasma TAFI and gene polymorphisms with DMA in Chinese patients with type 2 diabetes (T2D). Methods: Plasma TAFI of 223 patients with T2D was measured, and the genotypes and alleles of the 1040C/T, 438G/A, and 505G/A polymorphisms of the TAFI gene were analyzed. A ROC curve was constructed to evaluate the identifying power of TAFI between patients with T2D and DMA, and logistic regression analysis was used to observe the correlation of plasma TAFI and gene polymorphisms with the risk for DMA. Results: Plasma TAFI was higher in patients with DMA than in patients with only T2D (p < 0.05). TAFI exhibited the largest area under ROC in identifying diabetic kidney disease (DKD) from only T2D (0.763, 95% CI [0.674-0.853], p < 0.01), and adjusted multivariate analysis showed a high odds ratio (OR: 15.72, 95% CI [4.573-53.987], p < 0.001) for DKD. Higher frequencies of the CT genotype and T allele of the 1040C/T polymorphism were found in DKD compared with only T2D (respectively p < 0.05), and the CT genotype exhibited a high OR (1.623, 95% CI [1.173-2.710], p < 0.05) for DKD. DKD patients with the CT genotype had higher plasma TAFI levels, while T2D and DKD patients with CC/TT genotypes had lower plasma TAFI levels. Conclusion: Plasma TAFI and the CT genotype and T allele of the 1040C/T polymorphism are independent risk factors for DKD in Chinese T2D patients.


Assuntos
Carboxipeptidase B2 , Diabetes Mellitus Tipo 2 , Nefropatias Diabéticas , Humanos , Carboxipeptidase B2/genética , Diabetes Mellitus Tipo 2/complicações , Polimorfismo de Nucleotídeo Único/genética , Nefropatias Diabéticas/genética , População do Leste Asiático , Fatores de Risco
14.
Front Endocrinol (Lausanne) ; 14: 1186257, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-38027205

RESUMO

Objective: To investigate clinical and singleton newborn outcomes in fresh cycles of embryo transfer after intracytoplasmic sperm injection (ICSI-ET) with diverse sperm sources (ejaculate, epididymis, and testis) in patients with Oligoasthenospermia, obstructive azoospermia (OA) or non-obstructive azoospermia (NOA). Methods: Patients who received fresh ICSI-ET for the first time at the First Affiliated Hospital of Zhengzhou University Reproductive Medicine Center between June 2011 and June 2021 were selected for this 10-year retrospective cohort analysis. After propensity score matching, only 1630 cycles were included in the investigation of ICS-ET clinical and singleton newborn outcomes in patients with Oligoasthenospermia, OA, and NOA using sperm from diverse sperm sources. Results: After propensity score matching, our data revealed a negligible difference in baseline and cycle parameters among groups. In patients with Oligoasthenospermia and OA, different sperm sources do not appear to influence clinical pregnancy rates and live birth rates, nor do they influence newborn outcomes, such as newborn weight, premature birth rate, and neonatal sex ratio in singleton births, except for OA patients who use epididymal sperm having higher low birth weight (LBW) rates in singleton pregnancies than those who use testicular sperm. In addition, clinical pregnancy rates, live birth rates, singleton gestation birth weights, premature birth rates, and neonatal sex ratios were similar between patients with Oligoasthenospermia, OA, and NOA using testicular sperm. Conclusions: Regardless of the type of male infertility (Oligoasthenospermia, OA, NOA) or sperm sources (ejaculate, epididymis, testis), a successful ICSI-ET procedure can result in similar clinical and neonatal outcomes, such as clinical pregnancy rate, live birth rate, abortion rate, neonatal birth weight and sex ratio of singleton pregnancies.


Assuntos
Azoospermia , Infertilidade Masculina , Nascimento Prematuro , Gravidez , Recém-Nascido , Feminino , Humanos , Masculino , Injeções de Esperma Intracitoplásmicas/métodos , Estudos Retrospectivos , Nascimento Prematuro/etiologia , Sêmen , Infertilidade Masculina/terapia , Infertilidade Masculina/etiologia , Espermatozoides , Peso ao Nascer
15.
Adv Healthc Mater ; : e2303068, 2023 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-37972286

RESUMO

Premature ovarian failure (POF) features an upward incidence nowadays, and the human umbilical cord mesenchymal stem cells (hUC-MSCs)-derived exosomes (MSC-Exos) have shown applied values in the recovery of ovarian function. Here, a novel exosome-encapsulated microcarrier prepared by microfluidic technology for ovarian repair after chemotherapy damage is presented. The exosomes derived from lipopolysaccharide (LPS)-preconditioned hUC-MSCs are encapsulated with hyaluronic acid methacryloyl (HAMA) via microfluidic electrospray, which is named HAMA/MSC-Exos. Attributing to the biocompatibility and semipermeable property of HAMA, the encapsulated exosomes show great viability and controllable release behavior from HAMA. It is demonstrated that in situ transplantation of HAMA/MSC-Exos can rescue ovarian functions of cyclophosphamide-induced ovarian failure in mice by increasing ovarian volume, improving the number of antral follicles and restoring fertility. It is believed that the transplantation of HAMA/MSC-Exos will provide a new concept for the treatment of POF in clinical practice.

16.
Pharmacol Res ; 198: 107017, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-38006979

RESUMO

Sorafenib, a multi-targeted tyrosine kinase inhibitor, is a first-line treatment for advanced solid tumors, but it induces many adverse cardiovascular events, including myocardial infarction and heart failure. These cardiac defects can be mediated by alternative splicing of genes critical for heart function. Whether alternative splicing plays a role in sorafenib-induced cardiotoxicity remains unclear. Transcriptome of rat hearts or human cardiomyocytes treated with sorafenib was analyzed and validated to define alternatively spliced genes and their impact on cardiotoxicity. In rats, sorafenib caused severe cardiotoxicity with decreased left ventricular systolic pressure, elongated sarcomere, enlarged mitochondria and decreased ATP. This was associated with alternative splicing of hundreds of genes in the hearts, many of which were targets of a cardiac specific splicing factor, RBM20. Sorafenib inhibited RBM20 expression in both rat hearts and human cardiomyocytes. The splicing of RBM20's targets, SLC25A3 and FHOD3, was altered into fetal isoforms with decreased function. Upregulation of RBM20 during sorafenib treatment reversed the pathogenic splicing of SLC25A3 and FHOD3, and enhanced the phosphate transport into mitochondria by SLC25A3, ATP synthesis and cell survival.We envision this regulation may happen in many drug-induced cardiotoxicity, and represent a potential druggable pathway for mitigating sorafenib-induced cardiotoxicity.

17.
Artigo em Inglês | MEDLINE | ID: mdl-38030032

RESUMO

OBJECTIVE: The suicide risk in bipolar disorder (BD) is the highest among psychiatric disorders, and the neurobiological mechanism of suicide in BD remains unclear. The study aimed to investigate the underlying relevance between the implicated abnormalities of dynamic functional connectivity (FC) and suicide attempt (SA) in BD. METHODS: We used the sliding window method to analyze the dynamic FC patterns from resting-state functional MRI data in 81 healthy controls (HC) and 114 BD patients (50 with SA and 64 with none SA). Then, the temporal properties of dynamic FC and the relationship between altered measures and clinical variables were explored. RESULTS: We found that one of the five captured brain functional states was more associated with SA. The SA patients showed significantly increased fractional window and dwell time in the suicide-related state, along with increased number of state transitions compared with none SA (NSA). In addition, the connections within subcortical network-subcortical network (SubC-SubC), default mode network-subcortical network (DMN-SubC), and attention network-subcortical network (AN-SubC) were significantly changed in SA patients relative to NSA and HC in the suicide-related state. Crucially, the above-altered measures were significantly correlated with suicide risk. CONCLUSIONS: Our findings suggested that the impaired dynamic FC within SubC-SubC, DMN-SubC, and AN-SubC were the important underlying mechanism in understanding SA for BD patients. It highlights the temporal properties of whole-brain dynamic FC could serve as the valuable biomarker for suicide risk assessment in BD.

18.
Phys Chem Chem Phys ; 25(44): 30145-30171, 2023 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-37916298

RESUMO

Metamaterials are a kind of artificial material with special properties, showing huge potential for applications in fields such as infrared measurement, solar cells, optical sensors, and optical stealth. A metamaterial perfect absorber (MPA) is designed based on a metamaterial, featuring strong absorption, small volume, light weight, ultra-bandwidth, tunability and other characteristics. This paper introduces the absorption mechanism of MPAs from microwave to optical wave band, and four directions of absorber design are elaborated. Equivalent impedance matching, plasma resonance and interference effect are the main absorption mechanisms of MPA. Multiband perfect absorption, ultra-wideband and ultra-narrowband perfect absorption, polarization and angle insensitive absorption, and dynamically controllable tunable absorption are the main design aspects. Among them, the proposal of a dynamically tunable absorber realizes the dynamic absorption. Finally, the problems and challenges of metamaterial perfect absorber design are discussed.

19.
PLoS One ; 18(11): e0294521, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37972203

RESUMO

The cabbage white butterfly (Pieris rapae), a major agricultural pest, has become one of the most abundant and destructive butterflies in the world. It is widely distributed in a large variety of climates and terrains of China due to its strong adaptability. To gain insight into the population genetic characteristics of P. rapae in China, we resequenced the genome of 51 individuals from 19 areas throughout China. Using population genomics approaches, a dense variant map of P. rapae was observed, indicating a high level of polymorphism that could result in adaptation to a changing environment. The feature of the genetic structure suggested considerable genetic admixture in different geographical groups. Additionally, our analyses suggest that physical barriers may have played a more important role than geographic distance in driving genetic differentiation. Population history showed the effective population size of P. rapae was greatly affected by global temperature changes, with mild periods (i.e., temperatures warmer than those during glaciation but not excessively hot) leading to an increase in population size. Furthermore, by comparing populations from south and north China, we have identified selected genes related to sensing temperature, growth, neuromodulation and immune response, which may reveal the genetic basis of adaptation to different environments. Our study is the first to illustrate the genetic signatures of P. rapae in China at the population genomic level, providing fundamental knowledge of the genetic diversity and adaptation of P. rapae.


Assuntos
Brassica , Borboletas , Humanos , Animais , Borboletas/genética , Metagenômica , Biodiversidade , Temperatura , Variação Genética
20.
Hum Vaccin Immunother ; 19(3): 2277567, 2023 12 15.
Artigo em Inglês | MEDLINE | ID: mdl-37953301

RESUMO

Atherosclerosis (AS) is the main underlying cause of cardiovascular disease, and B cells are considered a key immune cell type to regulate AS. So far, there is no bibliometric study on B cell and AS. This study aims to comprehensively analyze the scientific output about B cell and AS, summarize the literature characteristics, explore research hotspots, and point out emerging trends. We searched the literature from 2003 to 2022 from the Web of Science Core Collection (WoSCC) database. CiteSpace, VOSviewer, and the R package "Bibliometrix" were used for literature analysis and visualization. A total of 1,062 articles and reviews were identified. The number of annual publications generally showed an upward trend. The United States and China were the most productive countries. Medical University of Vienna was the most productive research institution, and Binder Christoph J. was the most productive author, who was also from Medical University of Vienna. "Arteriosclerosis Thrombosis and Vascular Biology" was the most published journal and the most frequently cited journal. The most cited reference was written by Caligiuri G (2002) in "Journal of Clinical Investigation." The most frequent keywords were "inflammation," "macrophages," "cardiovascular disease," "T cells," "apoptosis," "immunity," "cytokines," "lymphocytes," etc. The trend topics were mainly focused on "immune infiltration," "immunoglobulins," and "biomarkers." The complex role of B cell subtypes and a variety of B cell mediators is the main research direction at present. In-depth analysis of B cell-specific targets can provide new ideas and methods for the prevention and treatment of AS.


Assuntos
Aterosclerose , Doenças Cardiovasculares , Humanos , Linfócitos B , Apoptose , Bibliometria
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