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1.
Adv Mater ; : e1908214, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32009282

RESUMO

Recently, electronic skin and smart textiles have attracted considerable attention. Flexible sensors, as a kind of indispensable components of flexible electronics, have been extensively studied. However, wearable airflow sensors capable of monitoring the environment airflow in real time are rarely reported. Herein, by mimicking the spider's fluff, an ultrasensitive and flexible all-textile airflow sensor based on fabric with in situ grown carbon nanotubes (CNTs) is developed. The fabric decorated with fluffy-like CNTs possesses exceptionally large contact area, endowing the airflow sensor with superior properties including ultralow detection limit (≈0.05 m s-1 ), multiangle airflow differential response (0°-90°), and fast response time (≈1.3 s). Besides, the fluffy fabric airflow sensor can be combined with a pristine fabric airflow sensor to realize highly sensitive detection in a wide airflow range (0.05-7.0 m s-1 ). Its potential applications including transmitting information according to Morse code by blowing the sensors, monitoring increasing and decreasing airflow velocity, and alerting blind people walking outside about potential hazard induced by nearby fast-moving objects are demonstrated. Furthermore, the airflow sensor can be directly integrated into clothing as stylish designs without sacrificing comfortness. It is believed that the ultrasensitive all-textile airflow sensor holds great promise for applications in smart textiles and wearable electronics.

2.
ACS Nano ; 2020 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-32083839

RESUMO

Protective clothing plays a vital role in safety and security. Traditional protective clothing can protect human body from physical injure. It is highly desired if modern wearable electronics can be integrated into traditional protection suit to endow it with versatile smart functions. However, it is still challenging to integrate electronics into clothing through a practical approach while keeping the intrinsic flexibility and breathability of textiles. In this work, we realized the direct writing of laser-induced graphene (LIG) on Kevlar textile in air and demonstrated the applications the as-prepared Janus graphene/Kevlar textile in intelligent protective clothing. The C=O and N-C bonds in Kevlar were broken and the remaining carbon atoms were reorganized into graphene, which can be ascribed to photothermal effect induced by the laser irradiation. Proof-of-concept devices based on the prepared graphene/Kevlar textile, including flexible Zn-air batteries, electrocardiogram electrodes, and NO2 sensors, were demonstrated. Furtherly, we fabricated a self-powered and intelligent protective clothing based on the graphene/Kevlar textile. The laser induced direct writing of graphene from commercial textiles in air conditions provides a versatile and rapid route for the fabrication of textile electronics.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32054269

RESUMO

Flexible electronics have gained considerable research concern due to their wide prospect for health monitoring, soft robotics and artificial intelligence, wherein flexible pressure sensors are necessary components of wearable devices. It is well known that the synergistic functions and multiscale structures of hybrid materials exert tremendous effects on the performance of flexible devices. Herein, inspired by the unique structure of the faceplate of sunflowers, we construct a hierarchical structure by in-situ grown vertically aligned molybdenum disulfide (MoS2) nanosheets on carbonized silk fabric (MoS2/CSilk), which is applied as the sensing material in flexible pressure sensors. The MoS2/CSilk pressure sen-sor showed high sensitivity and good stability. We demonstrated its applications in monitoring subtle physiology sig-nals, such as pulse wave and voice vibrations. In addition, they were used as electrodes in lithium ion batteries. The MoS2/CSilk electrode delivered ultra-high first-cycle discharge and charge capacities of 2895 and 1594 mA h g-1. The MoS2/CSilk electrode exhibited a high capacity of 810 mA h g-1 with a CE approaching 100% even after 300 cycles, sug-gesting a good high-rate stability. The excellent overall performances are attributed to the unique structure the MoS2/CSilk and the synergistic effect of CSilk and MoS2. The concept and strategy of this work can be extended to the design and fabrication of other multifunctional devices.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32028712

RESUMO

The initial concept of flood control has gradually shifted to flood risk management which emphasizes more public participation. Therefore, understanding the public's protective coping behavioral patterns to floods is significant, and can help improve the effectiveness of public participation and implementation of flood-mitigation measures. However, the quantitative effect of socio-demographic factors on flood risk perception and behaviors is not clear. In this study, the socio-demographic factors are included to explore the quantitative relationship with and the affect path to flood protective coping behaviors with socio-demographic factors are studied. Shenzhen City in China is chosen as the study area, which suffers frequent urban floods every year. Questionnaire surveys are conducted in five flood-prone communities there, and 339 valid questionnaires were collected. The correlations between flood risk perception, flood risk knowledge, flood risk attitude, socio-demographic factors, and protective coping behaviors are analyzed firstly. A structural equation model (SEM) about these factors is then established to verify the correctness of hypothetical paths and discover new paths. The results indicates that socio-demographic factors and flood risk perception do not have impacts on protective coping behaviors directly, but are mediated by flood risk knowledge and flood risk attitude. Flood risk attitude is an important factor that affects protective coping behaviors directly. Moreover, two affect paths to flood protective coping behaviors are proposed. The findings of Shenzhen city in this study can be extended to other cities with similar characteristics, providing support for conducting effective flood mitigation measures.

6.
Top Curr Chem (Cham) ; 378(1): 20, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32016608

RESUMO

Nucleic acids are considered not only extraordinary carriers of genetic information but also are perceived as the perfect elemental materials of molecular recognition and signal transduction/amplification for assembling programmable artificial reaction networks or circuits, which are similar to conventional electronic logic devices. Among these sophisticated DNA-based reaction networks, catalytic hairpin assembly (CHA), hybridization chain reaction (HCR), and DNAzyme represent the typical nonenzymatic amplification methods with high robustness and efficiency. Furthermore, their extensive hierarchically cascade integration into multi-layered autonomous DNA circuits establishes novel paradigms for constructing more different catalytic DNA nanostructures and for regenerating or replicating diverse molecular components with specific functions. Various DNA and inorganic nanoscaffolds have been used to realize the surface-confined DNA reaction networks with significant biomolecular sensing and signal-regulating functions in living cells. Especially, the specific aptamers and metal-ion-bridged duplex DNA nanostructures could extend their paradigms for detecting small molecules and proteins in even living entities. Herein, the varied enzyme-free DNA circuits are introduced in general with an extensive explanation of their underlying molecular reaction mechanisms. Challenges and outlook of the autonomous enzyme-free DNA circuits will also be discussed at the end of this chapter.


Assuntos
Técnicas Biossensoriais/métodos , DNA/química , DNA/metabolismo , DNA Catalítico/química , DNA Catalítico/metabolismo , Entropia , Transferência Ressonante de Energia de Fluorescência , Humanos , Microscopia de Fluorescência , Nanoestruturas/química , Hibridização de Ácido Nucleico
7.
J Cell Mol Med ; 2020 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-32073741

RESUMO

Early diagnosis of lung adenocarcinoma requires effective risk predictors. TNFRII was reported to be related to tumorigenesis, but remained unclear in lung cancer. This research set out to investigate the relationship between the sTNFRII (serum TNFRII) level and the risk of lung adenocarcinoma less than 1 cm in diameter. Seventy-one pairs of subcentimetre lung adenocarcinoma patients and healthy controls were analysed through multiplex bead-based Luminex assay and found a significantly lower expression of sTNFRII in patients with subcentimetre lung adenocarcinoma than that in the healthy controls (P < .001), which was further verified through ONCOMINE database analysis. Increased levels of sTNFRII reduced the risk of subcentimetre lung adenocarcinoma by 89% (P < .001). Patients with a higher level of BLC had a 2.70-fold (P < .01) higher risk of subcentimetre adenocarcinoma. Furthermore, a higher BLC/TNFRII ratio was related to a 35-fold higher risk of subcentimetre adenocarcinoma. TNFRII showed good specificity, sensitivity and accuracy (0.72, 0.75 and 0.73, respectively), with an AUC of 0.73 (P < .001). In conclusion, the present study assessed the value of sTNFRII as a potential biomarker to predict the risk of subcentimetre lung adenocarcinoma and provided evidence for the further use of TNFRII as an auxiliary marker in the diagnosis of subcentimetre lung adenocarcinoma.

8.
Biosens Bioelectron ; 151: 111985, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31999591

RESUMO

With the aggravation of sulfide pollution, more and more attention has been paid to the detection of sulfide in the environment. However, the detection of low-concentration sulfide is still a technical bottleneck to be solved urgently. In this study, a synergistic effect strategy that combines the co-catalysis of nanoporous gold (np-Au) and recombinant microbial cell with the excellent electrical conductivity of reduced graphene oxide (rGO) was proposed for the sensitive detection of low-concentration sulfide. A rGO/np-Au composite was fabricated and then used as an immobilization support for the bio-recognition element of recombinant Escherichia coli (E. coli) over-expressed sulfide: quinone oxidoreductase (SQR). A microbial biosensor (E. coliSQR/rGO/np-Au/GCE) was successfully constructed for the sensitive detection of low-concentration sulfide. Due to the synergistic effect of rGO, np-Au, and E. coliSQR cells, the sensitivity of the proposed microbial biosensor towards sulfide reached 400.42 µA mM-1 cm-2 with a wide linear response ranging from 100 nM to 7 mM, as well as a low detection limit of 98.5 nM using amperometric i-t curve method. Furthermore, the microbial biosensor was successfully applied to the detection of sulfide in wastewater with strong anti-interference ability, high reproducibility, and strong stability. These results confirmed that the proposed microbial biosensor was ideal for the detection of low-concentration sulfide in a reliable, specific, and sensitive way.

9.
Molecules ; 25(1)2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31906332

RESUMO

Astragalin, isolated from flowers of Rosa chinensis Jacq., is a kind of flavonoid, with anti-inflammatory, antioxidant, antiviral, analgesic, antibacterial, antiallergic, and antihepatotoxic effects. However, no studieson the procoagulant effect of astragalin have been reported. This study aimed to investigate the procoagulant activity of astragalin and its mechanism. Its procoagulant effect was investigated by activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and fibrinogen (FIB) in vitro, and a rat model established by heparin sodium was used to evaluate the mechanism for the procoagulant effect in vivo. The results showed that astragalin had good procoagulant effects compared with the control group in vitro. Compared with the model group in vivo, astragalin could shorten the coagulation time and significantly increase the number of platelets. Meanwhile, astragalin could significantly reduce the effectual time of PT and APTT and increase the content of FIB. The contents of 6-keto-PGF1α and eNOS significantly decreased. Astragalin could increase whole blood viscosity (WBV), plasma viscosity (PV), erythrocyte sedimentation rate (ESR) and packedcell volume (PCV). All of the above revealed that astragalin had good procoagulant effects by promoting the intrinsic and extrinsic coagulation system.

10.
Artigo em Inglês | MEDLINE | ID: mdl-31952331

RESUMO

Timely and secure evacuation of residents during flood disasters or other emergency events is an important issue in urban community flood risk management, especially in vulnerable communities. An agent-based modeling framework was proposed in order to indicate how the community properties (e.g., community density and percentage of vulnerable residents), residents' psychological attributes (e.g., flood risk tolerance threshold) and mutual aid mechanism affect the flood evacuation process. Results indicated that: (1) The community density negatively affected the flood evacuation efficiency. The greater the density of the community, the longer the evacuation time. (2) There was a negative correlation between the flood risk tolerance threshold of residents and evacuation efficiency. (3) The proportion of vulnerable resident agents had opposite effects on the evacuation efficiency of different types of communities, which was to negatively affect low-density communities and positively affect high-density communities. (4) Mutual aid mechanism can reduce evacuation time in low-density communities, and the effect was more pronounced with a higher proportion of vulnerable resident agents in the community. These findings can help managers to develop better emergency evacuation management for urban communities.

11.
Jpn J Clin Oncol ; 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31990345

RESUMO

BACKGROUND: This study aims to analyse the expression of human MOF in endometrial carcinoma cells and its relationship with estrogen and estrogen receptor and to investigate the effect of estrogen-human MOF on the malignant biological behaviours of endometrial carcinoma cells. METHODS: The expression of human MOF was detected in different endometrial tissues by immunohistochemistry. The effects of human MOF, human MOF combined with estrogen stimulation and estrogen plus anti-human MOF antibody blocking on the proliferation of endometrial carcinoma cells were evaluated by western blotting, real-time polymerase chain reaction, cell proliferation assay and cell cycle distribution. Bioinformatics was used to identify the correlations of human MOF and estrogen and involved pathways. RESULTS: The expression levels of human MOF in endometrial carcinoma tissues were significantly higher than that in atypical hyperplasia and normal endometrial tissues. High expression of human MOF was associated with late-stage cancer, lymph node metastasis and short survival time, and it was also an independent prognostic risk factor for endometrial carcinoma. After human MOF knockdown, the proliferation, migration and invasive capacity of Ishikawa cells decreased and cell apoptosis increased. After stimulation with estrogen, the PI3K/Akt and Ras-Raf-MEK-ERK signalling pathways were activated, and the expression of the human MOF protein was increased. human MOF (KAT8) expression showed a positive correlation with ESR1 expression, and KAT8-associated genes were enriched in the cell cycle pathways and splicing pathways. CONCLUSION: Human MOF was highly expressed in endometrial carcinoma and associated with proliferation. Estrogen/estrogen receptor enhanced human MOF expression; promoted the proliferation, migration and invasion of Ishikawa cells; and inhibited cell apoptosis by activating the PI3K/Akt and Ras-Raf-MEK-ERK signalling pathways.

12.
J Cardiovasc Electrophysiol ; 31(2): 560-569, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31919928

RESUMO

AIM: The aim of this study is to assess if left bundle branch pacing (LBBP) can preserve physiological cardiac synchrony and deliver favorable hemodynamic effects. METHODS: Consecutive patients undergoing dual chamber pacemaker implantation for sick sinus syndrome (SSS) and a normal cardiac function with a narrow QRS complex were recruited for the study. Electrocardiogram and echocardiographic examinations were performed during ventricular pacing-on and native-conduction modes. The QRS duration (QRSd), systolic dyssynchrony index (SDI), and the standard deviation of time-to-peak contraction velocity in left ventricular (LV) 12 segments (Tsd-12-LV) were measured to evaluate LV synchrony. The stroke volume (SV) and the degree of atrioventricular valvular regurgitation were also assessed. RESULTS: A total of 40 patients underwent LBBP, while another 38 patients underwent right ventricular septum pacing (RVSP) as control group. Baseline characteristics were similar between the two groups. With LBBP, the paced QRSd was slightly wider than the intrinsic QRSd (101.03 ± 8.79 ms vs 91.06 ± 14.17 ms, P < .0001) while the LV mechanical synchrony during LBBP pacing mode was similar to that of native-conduction mode (SDI, 3.14 ± 2.49 vs 2.70 ± 1.68, P = 0.129; Tsd-12-LV, 26.43 ± 15.55 vs 25.61 ± 16.07, P = .671) in the LBBP group. The LV synchrony in the LBBP group was superior to the RVSP group significantly. No significant differences in SV (64.08 ± 16.97 mL vs 65.45 ± 18.68 mL, P = .241) or the degree of atrioventricular valvular regurgitation were noted between LBBP capture and native-conduction modes. CONCLUSION: LBBP could preserve satisfactory LV synchrony and result in favorable hemodynamic effects.

13.
ACS Appl Mater Interfaces ; 12(5): 6112-6118, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31941273

RESUMO

Stretchable conductive fibers have gained significant attention in the field of wearable and flexible electronics because of their inherited unique properties. Up to now, there are few reports regarding the highly stretchable fibers with excellent electronic properties. In this work, a highly stretchable fiber with superior electrical conductivity is fabricated, which contains a core fiber, an intermediate modified layer, and an outer eutectic-gallium-indium liquid metal layer. The fiber demonstrates an excellent electrical conductivity of over 103 S cm-1 when stretched up to 500% strain, which is far superior to the existing stretchable conductive fiber. The stretchable conductive fiber shows excellent thermostability with a maximum operating temperature of nearly 250 °C. Such unique fibers can be applied as highly stretchable, deformable conductor to charge a mobile phone, and sensor to monitor human activities. This work offers promising application in the areas of flexible and wearable electronics.

14.
Anal Chim Acta ; 1097: 78-84, 2020 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-31910972

RESUMO

A biomimetic assembly of per-O-methylated-cyclodextrin dimer with cobalt proto-porphyrin (CoIII-PPIX@Py2CD) was achieved via covalent linkage between CoIII of CoIII-PPIX and pyridine N of Py2CD (primarily synthesized by the acyl chlorination reaction of two ß-CDs monomers with 3,5-bis (bromomethyl) pyridine). Ultraviolet-visible (UV-vis) and circular dichroism (CD) absorption spectroscopy, and NMR hydrogen spectroscopy (H1-NMR) were adopted to carefully characterize the structure of Py2CD and its functional assembly with CoIII-PPIX. X-ray photoelectron spectroscopy (XPS) was employed to affirm the binding of the as-obtained CoIII-PPIX@Py2CD, whose electrochemical kinetics were extensively studied to validate the feasibility in the catalytic reduction of hydrogen peroxide (H2O2). The developed sensor displayed the wide linear range for H2O2 detection and the low detection limit of 2.47 × 10-7 M. This work sheds some constructive lights on rational design and synthesis of preeminently biomimic carrier and high cost-effectiveness catalyst for (bio)analytical applications.

15.
Artigo em Inglês | MEDLINE | ID: mdl-31961985

RESUMO

DNAzymes have been recognized as promising transducing agents for visualizing endogenous biomarkers, but their inefficient intracellular delivery and limited amplification capacity (including insufficient cofactor supply) preclude their extensive biological application. Herein, an autocatalytic DNAzyme (ACD) biocircuit is constructed for amplified microRNA imaging in vivo based on a hybridization chain reaction (HCR) and DNAzyme biocatalysis, sustained by a honeycomb MnO2 nanosponge (hMNS). The hMNS not only delivers DNA probes, but also supplies Mn2+ as a DNAzyme cofactor and magnetic resonance imaging (MRI) agent. Through the subsequent cross-activation of HCR and DNAzyme amplicons, the ACD amplifies the limited signal resulting from miRNA recognition. The hMNS/ACD system was used to image microRNA in vivo, thus demonstrating its great promise in cancer diagnosis.

16.
Anal Chem ; 92(4): 3206-3212, 2020 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-31939299

RESUMO

Novel and distinct enhancement in electrochemiluminescence (ECL) signals of advanced organic luminophores are of importance for expanding their applications in early diagnosis. This work reported the construction of an ultrasensitive label-free ECL aptasensor for thrombin (TB) detection by grafting zinc proto-porphyrin IX (ZnP) onto an aminated zeolitic imidazole framework-8 (defined as ZnP-NH-ZIF-8 for clarity) as the luminophore. The structure and optical properties of the resulting ZnP-NH-ZIF-8 were carefully characterized. For that, there appeared to be weak ECL radiation for ZnP in dichloromethane (DCM) containing tetra-n-butylammonium perchlorate (TBAP) because of the as-formed singlet-state oxygen via the "reduction-oxidation" route. More notably, the ECL signals display 153-times enhancement for ZnP-NH-ZIF-8, thanks to the excellent catalytic kinetics for the oxygen reduction reaction (ORR). By virtue of the specific interactions of the TB aptamer (TBA) with the TB protein and the highly efficient catalysis of the ZnP-NH-ZIF-8 for ORR, the as-prepared aptasensor showed a wider linear range (0.1 fM∼1 pM) and a lower detection limit (ca. 58.6 aM). This work provides some useful guidelines for synthesis of an advanced organic luminophore with largely boosted ECL signals in ultrasensitive analysis and clinical diagnosis.

17.
Food Chem Toxicol ; 135: 110993, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31765702

RESUMO

3S, 3'S-Astaxanthin is the most powerful antioxidant to scavenge free radicals in the world. In this study, a 3S, 3'S-astaxanthin biosynthesis pathway was constructed in a probiotic yeast, Kluveromyces marxianus, denoted YEAST, and its bioactive metabolites were extracted for biofunctional assessments. The bio-safety examination was achieved by two animal models as following: First, no significant toxic effects on YEAST groups were found in zebrafish; Second, after feeding YEAST for 4 weeks, the rat-groups showed no visible abnormality, and no significant change of the body weight and blood biochemistry tests. The inhibition of lung metastasis of melanoma cells and the increment of the survival rate were demonstrated by feeding YEAST and injecting the intravenous commercial astaxanthin in vivo rodent model. Based on in vitro assays of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging analysis, ferrous ion chelating ability, reducing power assessment, and mushroom tyrosinase inhibition evaluation, YEAST-astaxanthin showed anti-oxidative and tyrosinase suppressive properties. Taken together, the 3S, 3'S-astaxanthin producing probiotic yeast is safe to be used in the bio-synthesis of functional and pharmaceutical compounds, which have broad industrial applications on cosmetic, food and feed additive and healthcare.

18.
J Hazard Mater ; 382: 120987, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31454609

RESUMO

Hyperspectral imaging, with the hundreds of bands and high spectral resolution, offers a promising approach for estimation of heavy metal concentration in agricultural soils. Using airborne imagery over a large-scale area for fast retrieval is of great importance for environmental monitoring and further decision support. However, few studies have focused on the estimation of soil heavy metal concentration by airborne hyperspectral imaging. In this study, we utilized the airborne hyperspectral data in LiuXin Mine of China obtained from HySpex VNIR-1600 and HySpex SWIR-384 sensor to establish the spectral-analysis-based model for retrieval of heavy metals concentration. Firstly, sixty soil samples were collected in situ, and their heavy metal concentrations (Cr, Cu, Pb) were determined by inductively coupled plasma-mass spectrometry analysis. Due to mixed pixels widespread in airborne hyperspectral images, spectral unmixing was conducted to obtain purer spectra of the soil and to improve the estimation accuracy. Ten of estimated models, including four different random forest models (RF)-standard random forest (SRF), regularized random forest (RRF), guided random forest (GRF), and guided regularized random forest (GRRF)-were introduced for hyperspectral estimated model in this paper. Compared with the estimation results, the best accuracy for Cr, Cu, and Pb is obtained by RF. It shows that RF can predict the three heavy metals better than other models in this area. For Cr, Cu, Pb, the best model of RF yields Rp2 values of 0.75,0.68 and 0.74 respectively, and the values of RMSEp are 5.62, 8.24, and 2.81 (mg/kg), respectively. The experiments show the average estimated values are close to the truth condition and the high estimated values concentrated near several industries, valifating the effectiveness of the presented method.

19.
Chemosphere ; 240: 124851, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31546187

RESUMO

Cadmium (Cd) is a toxic heavy metal and its uptake by living organisms causes adverse effect, further resulting in cycle pollution of the biosphere. The specific regulatory mechanism between clays and microbes under Cd stress remains unclear. In this study, interface interactions among clays, microbes and Cd were confirmed. Comparative transcriptome was conducted to investigate how it regulated gene expression patterns of microbes (Chryseobacterium sp. WAL2), which exposed to a series of gradient concentrations of Cd (16, 32, 64 and 128 µg mL-1) for 12 d in the presence and absence of clay montmorillonite (Mt) (16 g L-1). Cd was highly enriched by the unique interface interactions between Mt and bacteria (67.6-82.1%), leading to a more hostile environment for bacterial cells. However, Mt ultimately enhanced bacterial resistance to Cd stress by stimulating the mechanism of bacterial resistance; namely: (i) Mt increased genes expression connected with ion transport, enhancing the uptake of Cd; (ii) Mt stimulated genes expression related to efflux pump and positively regulated cellular oxidative stress (e.g., glutathione) and Cd accumulation (e.g., cysteine) processes. Further, genes expression related to intracellular metabolic processes was enforced, which supplied a driving force and accelerated electron transfer; (iii) Mt improved genes expression involved in DNA replication and other biological processes (e.g., terpenoid backbone biosynthesis) to maintain bacterial vitality. Therefore, the study not only optimized a unique Cd resistance mechanism of Mt on Chryseobacterium sp., but also provided a novel insight for environmental mitigation of heavy metals from the perspective of molecular biology.


Assuntos
Proteínas de Bactérias/genética , Bentonita/farmacologia , Cádmio/toxicidade , Chryseobacterium/efeitos dos fármacos , Chryseobacterium/fisiologia , Farmacorresistência Bacteriana/efeitos dos fármacos , Proteínas de Bactérias/metabolismo , Cádmio/farmacocinética , Chryseobacterium/química , Farmacorresistência Bacteriana/genética , Perfilação da Expressão Gênica , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Microbiologia do Solo , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
20.
Food Chem Toxicol ; 136: 111050, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31843533

RESUMO

The pickled radish can be kept at room temperature for years without spoilage. 2,6-dihydroxyacetophenone (DHAP), 4-hydroxybenzaldehyde (HBA), and 4-hydroxyphenethyl alcohol (4-HPEA) were first found from the pickled radish. The structures of three phenolic compounds were elucidated by analysis of their nuclear magnetic resonance and high-resolution electro-spray ionization mass spectrometry data. All these phenolic compounds showed good free radical scavenging capacity except HBA. Both DHAP and 4-HPEA also showed high ferric reducing ability. DHAP showed good antimicrobial activity against Escherichia coli, Bacillus subtilis, and Canidia albicans. HBA demonstrated antimicrobial activity against E. coli and C. albicans but not B. subtilis. Based on the results of MTT assay, these compounds did not show cytotoxicity to LO2 cell line. All results indicated the pickled radish had antioxidant and antimicrobial phenolic compounds. To the best of our knowledge, this report is the first to answer partially the question of why pickled foods can be kept at room temperature for years without spoilage based on the evidence of three phenolic compounds.

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