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1.
Lab Med ; 2019 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-31150544

RESUMO

OBJECTIVE: To assess the rate of, and risk factors for, human cytomegalovirus viremia (HCMV) in donor+/recipient+ (HCMV serostatus matched) hematopoietic stem-cell transplantation (HSCT) recipients. METHODS: HCMV DNA from 144 donor+/recipient+ HSCT recipients was examined by quantitative polymerase chain reaction (qPCR). RESULTS: The cumulative incidence of HCMV viremia was 69.4% (100/144) during the 48 weeks after HSCT. In a multivariate analysis, acute graft-versus-host disease (aGVHD) was discovered to be a risk factor for the occurrence of HCMV viremia (P = .006). The cumulative incidence of HCMV viremia and increasing DNA loads were significantly associated with aGVHD occurrence (P = .001 for each). The occurrence of late-term HCMV viremia was associated with aGVHD (P = .001) and a higher DNA load during the first 12 weeks after HSCT (P = .04). CONCLUSIONS: aGVHD is a risk factor for HCMV viremia. Recipients with aGVHD who have a high HCMV DNA load should be strictly monitored to prevent HCMV activation.

2.
Biochem Biophys Res Commun ; 514(1): 329-335, 2019 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-31036319

RESUMO

Endothelial dysfunction plays an important role in the pathogenesis of acute lung injury (ALI). Tetramethylpyrazine (TMP) has been reported to attenuate harmful changes in ALI rats. However, the effects of TMP on endothelial cell injury and its underlying mechanisms remain unknown. In this study, human umbilical vein endothelial cells (HUVECs) induced by lipopolysaccharide (LPS) was used as an inflammatory injury model, also served as LPS group. HUVECs pretreated with TMP for 2 h before induced by LPS was served as LPS + TMP group. Untreated HUVECs was served as control group. After incubation with LPS for 12 h, cell viability and morphology, cell apoptosis rate, CD31-positive endothelial microparticles (EMPs) release, proinflammatory cytokines secretion, and ROCK II expression were evaluated. Compared with LPS group, TMP pretreatment improved cell viability and morphology. Besides, cell apoptosis rate, CD31-positive EMPs amount, TNF-α and IL-1ß concentrates, and ROCK II mRNA and protein levels in LPS + TMP group were significantly decreased when compared with LPS group. To further confirm the mechanism, HUVECs in all the above groups were pretreated with Y27632 (ROCK II inhibitor) for 30 min before grouping, then treated as above. No significant differences in cell apoptosis rate, CD31-positive EMPs amount, and ROCK II expression between Y27632 + LPS group and Y27632 + LPS + TMP group were found. To sum up, our study found that TMP alleviated LPS-induced inflammatory injury in HUVECs by inhibiting Rho/ROCK pathway.

3.
Nanoscale ; 11(15): 7247-7255, 2019 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-30931441

RESUMO

A simple and low-cost approach is developed, by which nitrogen-doped carbon dots (NCDs) with a negative potential are assembled inside the mesoporous channels of SBA-15 via capillary force. The unique confined microenvironment leads to a strong interaction between confined NCDs and the inner surface of SBA-15, thus effectively avoiding the aggregation of NCDs. The resultant composite (NCDs-in-SBA-15) exhibits blue fluorescence similar to the NCD aqueous solution, and shows excellent structural, thermal and photostability. Solid NCDs-in-SBA-15 still emits fluorescence even after heat treatment at 400 °C under ambient atmosphere. In addition, NCDs-in-SBA-15 possesses remarkable resistance to acid/alkali solvents. Furthermore, NCDs-in-SBA-15 shows superior selectivity and adsorption capacity to Fe3+. The facile approach and these advantageous performances could make CDs meet the requirements of fluorescent materials in the solid state and then have wider applications.

4.
Inorg Chem ; 58(9): 5746-5752, 2019 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-30950600

RESUMO

To better utilize carbon dots (CDs) as efficient photocatalysts, an excellent strategy of constructing CDs@MoS2 heterostructure is presented. Here a facile sonication-hydrothermal method is utilized to synthesize CDs@MoS2. Such heterostructure regulates the energy level configuration, and visible light absorption and the separation and transfer of photogenerated charges are enhanced remarkably, which is propitious for the production of more photoinduced charges and improvement of the heterogeneous photocatalytic activity. Meanwhile, the photocatalytic performance of CDs@MoS2 was obviously improved in methylene blue degradation. On the basis of a series of contrast experiments, the possible mechanism of the photocatalytic reaction is proposed. Therefore, this work offers a facile route for the design of a zero-dimensional/two-dimensional heterojunction for the adjustment of the energy level structure and the improvement in photocatalytic performance.

5.
J Agric Food Chem ; 67(6): 1749-1759, 2019 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-30657680

RESUMO

The aim of this work was to produce a series of phytosteryl amino acid ester hydrochlorides by a two-step method, which involved esterification of phytosterols with N- tert-butoxycarbonyl (BOC) amino acid and deprotection of the BOC group. The highest yield of over 95.0% was obtained when the catalysts were the mixtures of 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide hydrochlide, 4-dimethylaminopyridine, and triethylamine. It was found that batch charging of the reactants and catalysts was conducive to improving the yield. In addition, over 99.0% of the BOC group deprotection degree was achieved using the HCl/ethyl acetate deprotection method. All of the compounds were characterized by fourier transform infrared spectroscopy, mass spectroscopy, and nuclear magnetic resonance spectroscopy. The emulsifying properties of phytosterols and phytosteryl amino acid ester hydrochlorides were also investigated. The results showed higher emulsifying properties of phytosteryl amino acid ester hydrochlorides, which could favor its wide application in food systems.


Assuntos
Acetatos/química , Aminoácidos/química , Emulsificantes/química , Ésteres/química , Ácido Clorídrico/química , Fitosteróis/química , Catálise , Espectroscopia de Ressonância Magnética , Espectroscopia de Infravermelho com Transformada de Fourier
6.
Small ; 14(44): e1803447, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30286281

RESUMO

A photocatalytic reaction is always governed by energy band configuration of the catalyst, but its modulation is challenging. Here, the adjustment of band-edge positions of Ag3 PO4 through fluorescent carbon dots is reported for the first time. Both Ag3 PO4 and carbon dots which keep the similar sizes constitute a heterojunction. Such heterostructure not only promotes visible light absorption, photogenerated charge separation, and transfer, but it also transforms photocatalytic activity of Ag3 PO4 from photooxidation to photoreduction due to the huge changes of band-edge positions. In addition, the heterojunction structure of carbon dots and Ag3 PO4 exhibits unique temperature-responsive photocatalytic activities and higher photocatalytic stability than the pure Ag3 PO4 . According to the contrast experiments and related characterizations, the possible mechanism of photocatalytic reaction is proposed. Therefore, this work offers an alternative route for adjusting energy band positions or tuning photocatalytic performance as well as for preparing novel carbon-dot-based heterostructure.

7.
Dalton Trans ; 47(36): 12694-12701, 2018 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-30140823

RESUMO

Hybrid nanostructures comprising disparate materials and having intricate shapes and facets are highly desired in photocatalytic applications requiring high selectivity. However, to produce such structures requires precise morphological control, and this is largely dictated by the stringent requirements of the reaction conditions, in addition to intricate, expensive, or tedious preparation steps. We present a simple, inexpensive, and scalable method for the preparation of hybrid nanostructures that combines carbon dots with hexagonal nanoplates of nickel-based compounds. The resultant heterostructure not only improves visible light absorption, but also exhibits enhanced photocatalytic activity for the reduction of 4-nitrophenol to 4-aminophenol when compared to carbon dots or hexagonal nanoplates of nickel-based compounds alone. The enhanced photocatalytic activity is due to the higher separation and transfer efficiency of photoexcited charge carriers at the interface between the carbon dot and hexagonal nanoplate of nickel-based compounds. This work opens up a straightforward and effective route to yield inexpensive and efficient photocatalysts for improved solar energy capture and conversion.

8.
J Chromatogr A ; 1566: 23-31, 2018 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-29954610

RESUMO

In this study, a hybrid composite containing melamine-based covalent organic polymer (MCOP) and ionic liquid (IL) was designed and employed for the functionalization of polymer monolith. The obtained polymer monolithic material was confirmed by scanning electron microscopy, Fourier-transformed infrared spectroscopy, X-ray photoelectron spectroscopy, and thermal gravimetric analysis. A polymer monolith microextraction (PMME) strategy was established based on the monolith for the enrichment of synthetic phenolic antioxidants, exhibiting high sensitivity, good stability and repeatability. Intra-day and inter-day relative standard deviations were both less than 10%. The developed PMME-HPLC method was applied to the preconcentration and determination of synthetic phenolic antioxidants in cosmetic samples and assessed through recovery studies. The developed polymer monolithic material had great potential as a candidate in the preconcentration field for PMME.


Assuntos
Antioxidantes/isolamento & purificação , Técnicas de Química Analítica/métodos , Cosméticos/química , Líquidos Iônicos/química , Fenóis/isolamento & purificação , Polímeros/química , Triazinas/química , Cromatografia Líquida de Alta Pressão
9.
Front Microbiol ; 9: 957, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29867862

RESUMO

Bacterial ß-galactosidase is involved in lactose metabolism and acts as a prevalent reporter enzyme used in studying the activities of prokaryotic and eukaryotic promoters. Xanthomonas campestris pv. campestris (Xcc) is the pathogen of black rot disease in crucifers. ß-Galactosidase activity can be detected in Xcc culture, which makes Escherichia coli LacZ unable to be used as a reporter enzyme in Xcc. To systemically understand the ß-galactosidase in Xcc and construct a ß-galactosidase -deficient strain for promoter activity analysis using LacZ as a reporter, we here analyzed the putative ß-galactosidases in Xcc 8004. As glycosyl hydrolase (GH) family 2 (GH2) and 35 (GH35) family enzymes were reported to have beta-galactosidase activities, we studied all of them encoded by Xcc 8004. When expressed in E. coli, only two of the enzymes, XC1214 and XC2985, were found to have ß-galactosidase activity. When deleted from the Xcc 8004 genome, only the XC1214 mutant had no ß-galactosidase activity, and other GH2 and GH35 gene deletions resulted in no significant reduction in ß-galactosidase activity. Therefore, XC1214 is the main ß-galactosidase in Xcc 8004. Notably, we have constructed a ß-galactosidase-free strain that can be employed in gene traps using LacZ as a reporter in Xcc. The results reported herein should facilitate the development of high-capacity screening assays that utilize the LacZ reporter system in Xcc.

10.
Front Chem ; 6: 186, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29888223

RESUMO

A new class of freestanding cross-linked hybrid polymer electrolytes (HPEs) with POSS as the cross-linker was prepared by a one-step free radical polymerization reaction. Octavinyl octasilsesquioxane (OV-POSS) with eight functional corner groups was used to provide cross-linking sites for the connection of polymer segments and the required mechanical strength to separate the cathode and anode. The unique cross-linked structure offers additional free volume for the motion of EO chains and provides fast and continuously interconnected ion-conducting channels along the nanoparticles/polymer matrix interface. The HPE exhibits the highest ionic conductivity of 1.39 × 10-3 S cm-1, as well as excellent interfacial compatibility with the Li electrode at 80°C. In particular, LiFePO4/Li cells based on the HPE deliver good rate capability and long-term cycling performance with an initial discharge capacity of 152.1 mAh g-1 and a capacity retention ratio of 88% after 150 cycles with a current density of 0.5 C at 80°C, demonstrating great potential application in high-performance LIBs at elevated temperatures.

11.
Food Chem ; 263: 321-326, 2018 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-29784324

RESUMO

In this study, a one-step method for producing phytosteryl phenolates, namely phytosteryl cinnamate and ferulate, has been successfully developed and their chemical structures were confirmed by FT-IR, MS and NMR. The highest yield of phytosteryl ferulate (85.7%) was obtained at 100 °C for 2 h after optimization. A Lewis acid catalyst scandium triflate was selected as the catalyst, and it turned out that it could be reused for at least five times without significant loss of activity. Meanwhile, it has been demonstrated that the solubility of phytosteryl phenolates in soybean oil was much higher than in both phenolic acid and phytosterol, which was conducive to expand their applications in oil-based food. The research finding helps realize convenient, green and efficient synthesis of phytosteryl phenolates.


Assuntos
Hidroxibenzoatos/análise , Fitosteróis/química , Catálise , Cromatografia Líquida de Alta Pressão , Cobre/química , Ácidos Cumáricos/química , Hidroxibenzoatos/química , Espectroscopia de Ressonância Magnética , Espectrometria de Massas por Ionização por Electrospray , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura Ambiente
12.
Oncol Lett ; 15(1): 292-296, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29422960

RESUMO

Plant secondary metabolites including alkaloids, demonstrate a complex diversity in their molecular scaffolds and exhibit tremendous pharmacological potential as anti-cancerous therapeutics. The present study aimed to evaluate the anticancer activity of a natural alkaloid, mecambridine, against human oral squamous cell carcinoma (OSCC). An MTT assay was used to evaluate cytotoxic effects of mecambridine on HSC-3 oral squamous cell carcinoma cells. Effects of mecambridine on autophagy-associated proteins were analyzed by western blotting. Effects on reactive oxygen species (ROS) and mitochondrial membrane potential were assessed by flow cytometry. Results indicated that mecambridine exhibited an IC50 value of 50 µM and exerted its cytotoxic effects in a dose dependent manner on OSCC HSC-3 cells. Furthermore, it was observed that mecambridine decreases cell viability and induces autophagy in a dose-dependent manner. The underlying mechanism for the induction of autophagy was demonstrated to be associated with ROS-mediated alterations in mitochondrial membrane potential and modulation of the mechanistic target of rapamycin/phosphoinositide 3-kinase/protein kinase B (m-TOR/PI3K/Akt) signaling pathway in HSC-3 at the IC50. In conclusion, the present study suggests that mecambridine exhibits substantial anticancer activity against OSCC HSC-3 cells by induction of autophagy and modulates the expression of the mTOR/PI3K/Akt signaling cascade which is considered a potential target pathway for anti-cancer agents.

13.
Food Chem ; 240: 736-742, 2018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-28946336

RESUMO

In this work, an enzymatic route for synthesizing phytosteryl lipoate was successfully set up for the first time. The structure of final product phytosteryl lipoate was determined by Fourier Transform Infrared (FTIR), Mass Spectrometry (MS) and Nuclear Magnetic Resonance (NMR). The maximum conversion of 71.2% was achieved when the following conditions were employed: 150mmol/L phytosterol, 1: 2.5M ratio of phytosterol to lipoic acid, 10g/L of 4Å molecular sieves and 60g/L Candida rugosa in 2-methyl-2-butanol/n-hexane (1:1, v/v) at 55°C for 96h. The physicochemical properties including solubility and antioxidant ability of phytosteryl lipoate in oil were assessed. The results revealed that phytosteryl lipoate possessed over twice as much oil solubility as free phytosterol and also showed better antioxidant ability. Investigation on its biological functions will be the main object in the future work.


Assuntos
Antioxidantes/metabolismo , Hexanos , Pentanóis , Fitosteróis
14.
Mol Med Rep ; 15(6): 3898-3904, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28440401

RESUMO

In the present study, clonal amplifications of T-cell receptor ß variable (TCR BV) linked to human cytomegalovirus (HCMV) infection were detected in recipients of allogeneic hematopoietic stem cell transplants (HSCT), and certain relationships between them were identified. Furthermore, the relationship between TCR BV sequences and HCMV infections was investigated. The results indicated that the 3 recipients of HSCT had monoclonal expansion of specific TCR BV clones following HSCT. Among these recipients, 2 suffered from pp65 and immediate early (IE) antigenemia. These patients demonstrated preferential expansion of TCR BV9 (QVRGGTDTQ) and TCR BV11 (VATDFQ). The remaining recipient did not express TCR BV9 and TCR BV11, nor did this individual have pp65 and IE antigenemia. These results suggest that expression of TCR BV9 and TCR BV11 may be associated with HCMV antigenemia, and may be involved in the immune response. The amino acid sequences 'QVRGGTDTQ' and 'VATDFQ' may be involved in HCMV reactivation in patients who have undergone HSCT. Assessment of the TCR BV families may provide valuable insight into HCMV pathogenesis and may aid in the diagnosis and therapy for HSCT recipients infected with HCMV.


Assuntos
Infecções por Citomegalovirus/etiologia , Citomegalovirus , Suscetibilidade a Doenças , Transplante de Células-Tronco Hematopoéticas , Receptores de Antígenos de Linfócitos T alfa-beta/genética , Adulto , Anticorpos Antivirais/imunologia , Comorbidade , Regiões Determinantes de Complementaridade/química , Regiões Determinantes de Complementaridade/genética , Citomegalovirus/imunologia , Infecções por Citomegalovirus/imunologia , Infecções por Citomegalovirus/virologia , Feminino , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Humanos , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Imunoglobulina M/sangue , Imunoglobulina M/imunologia , Imunossupressores/efeitos adversos , Masculino , Fosfoproteínas/imunologia , Receptores de Antígenos de Linfócitos T alfa-beta/química , Receptores de Antígenos de Linfócitos T alfa-beta/metabolismo , Análise de Sequência de DNA , Condicionamento Pré-Transplante/efeitos adversos , Condicionamento Pré-Transplante/métodos , Transplante Homólogo , Proteínas da Matriz Viral/imunologia , Adulto Jovem
15.
Biomed Res Int ; 2017: 8314276, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29445744

RESUMO

P21 activated kinase 4 (PAK4), a key regulator of cytoskeletal rearrangement and endothelial microparticles (EMPs), is released after lipopolysaccharide (LPS) stimulation. In addition, it participates in LPS-induced lung injury. In this study, forty-eight Sprague Dawley (SD) rats were divided into two groups, including PAK4 inhibitor (P) and PAK4 inhibitor + simvastatin (P + S) treatment groups. All rats were given PAK4 inhibitor (15 mg/kg/d) orally. Immediately after PAK4 inhibitor administration, simvastatin was injected intraperitoneally to P + S group animals at 20 mg/kg/day. Then, treatment effects on the intestinal mucosal barrier and lung injury caused by PAK4 inhibitor and simvastatin were assessed. The results showed that gut Zonula Occludens- (ZO-) 1, PAK4, mitogen-activated protein kinase 4 (MPAK4), and CD11c protein levels were reduced, while plasma endotoxin levels were increased after administration of PAK4 inhibitor. Furthermore, compared with normal rats, wet-to-dry (W/D) values of lung tissues and circulating EMP levels were increased in the treatment group, while PAK4 and CD11c protein amounts were reduced. Therefore, in this lung injury process induced by PAK4 inhibitor, the protective effects of simvastatin were reflected by intestinal mucosal barrier protection, inflammatory response regulation via CD11c+ cells, and cytoskeleton stabilization. In summary, PAK4 is a key regulator in the pathophysiological process of acute lung injury (ALI) and can be a useful target for ALI treatment.


Assuntos
Lesão Pulmonar Aguda/tratamento farmacológico , Intestinos/efeitos dos fármacos , Sinvastatina/administração & dosagem , Quinases Ativadas por p21/antagonistas & inibidores , Lesão Pulmonar Aguda/induzido quimicamente , Lesão Pulmonar Aguda/patologia , Animais , Antígeno CD11c/genética , Modelos Animais de Doenças , Inibidores Enzimáticos/efeitos adversos , Humanos , Intestinos/lesões , Intestinos/patologia , Lipopolissacarídeos/toxicidade , Ratos , Fator de Necrose Tumoral alfa/genética , Quinases Ativadas por p21/genética
16.
Int J Infect Dis ; 46: 89-93, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-27057748

RESUMO

BACKGROUND: Viral infections are a major cause of morbidity and mortality after hematopoietic stem cell transplantation (HSCT). The effect of herpesvirus infections in human cytomegalovirus (HCMV)-seropositive (IgG-positive/IgM-negative) HSCT recipients remains poorly understood. The risk factors associated with Epstein-Barr virus (EBV), HCMV, and human herpes virus type 6 (HHV-6) infections after HSCT, both alone and in combination, were investigated in this study. METHODS: Peripheral blood specimens were collected from 44 HSCT recipients and examined for viral DNA using quantitative fluorescence PCR assays. Risk factors for EBV, HCMV, and HHV-6 infections were analyzed by binary logistic regression, and relationships between these viruses were analyzed using the Chi-square test. RESULTS: EBV, HCMV, and HHV-6 were detected in 50%, 45.45%, and 25% of HCMV-seropositive (IgG-positive/IgM-negative) HSCT recipients, respectively. Male sex (p=0.007) and conditioning regimens including anti-thymocyte globulin (ATG) (p=0.034) were strongly associated with an increased risk of EBV infection. Graft-versus-host disease (GVHD) prophylaxis with corticosteroids was a risk factor for both EBV (p=0.013) and HCMV (p=0.040) infections, while EBV infection (p=0.029) was found to be an independent risk factor for HHV-6 infection. Pre-existing HHV-6 infection was associated with lower rates of HCMV infection (p=0.002); similarly, pre-existing HCMV infection was protective against HHV-6 infection (p=0.036). CONCLUSIONS: HCMV-seropositive (IgG-positive/IgM-negative) HSCT recipients exhibited a high rate of herpesvirus infections, particularly EBV. ATG and male sex were strongly associated with an increased risk of EBV infection. GVHD prophylaxis with prednisone was found to affect both EBV and HCMV infections. Prior infection with EBV was shown to promote HHV-6 infection. Taken together, these data highlight the need for active monitoring of herpesvirus infections in patients undergoing HSCT.


Assuntos
Infecções por Citomegalovirus/virologia , Citomegalovirus/imunologia , Infecções por Vírus Epstein-Barr/virologia , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Infecções por Herpesviridae/virologia , Complicações Pós-Operatórias/virologia , Adolescente , Adulto , Anticorpos Antivirais/sangue , Citomegalovirus/genética , Citomegalovirus/isolamento & purificação , Infecções por Citomegalovirus/sangue , Infecções por Citomegalovirus/etiologia , Infecções por Vírus Epstein-Barr/sangue , Infecções por Vírus Epstein-Barr/etiologia , Feminino , Infecções por Herpesviridae/sangue , Infecções por Herpesviridae/etiologia , Humanos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/sangue , Complicações Pós-Operatórias/etiologia , Transplantados/estatística & dados numéricos , Adulto Jovem
17.
PLoS One ; 10(10): e0139825, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26431420

RESUMO

OBJECTIVES: To compare cardiopulmonary resuscitation (CPR) quality between manual CPR and miniaturized chest compressor (MCC) CPR. To improve CPR quality through evaluating the quality of our clinical work of resuscitation by real-time video recording system. METHODS: The study was a retrospective observational study of adult patients who experienced CPR at the emergency department of Shanghai Tenth People's Hospital from March 2013 to August 2014. All the performance of CPR were checked back by the record of "digital real-time video recording system". Average chest compression rate, actual chest compression rate, the percentage of hands-off period, time lag from patient arrival to chest compression, time lag from patient arrival to manual ventilation, time lag from patient arrival to first IV establish were compared. Causes of chest compression hands-off time were also studied. RESULTS: 112 cases of resuscitation attempts were obtained. Average chest compression rate was over 100 compression per minute (cpm) in the majority of cases. However, indicators such as percentage of hands-off periods, time lag from patient arrival to the first manual ventilation and time lag from patient arrival to the first IV establish seemed to be worse in the manual CPR group compared to MCC CPR group. The saving of operators change time seemed to counteract the time spent on MCC equipment. Indicators such as percentage of hands-off periods, time lag between patient arrival to the first chest compression, time lag between patient arrival to the first manual ventilation and time lag from patient arrival to the first IV establish may influence the survival. CONCLUSION: Our CPR quality remained to be improved. MCC may have a potentially positive role in CPR.


Assuntos
Reanimação Cardiopulmonar/instrumentação , Reanimação Cardiopulmonar/métodos , Gravação em Vídeo/instrumentação , Gravação em Vídeo/métodos , Oscilação da Parede Torácica/métodos , Serviço Hospitalar de Emergência , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
18.
Respir Care ; 60(11): 1635-42, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26264418

RESUMO

BACKGROUND: The objective was to study the level of monocyte-human leukocyte antigen-DR (mHLA-DR), an immune function-related biomarker, at 24 h after admission, to predict the outcomes of subjects with severe pneumonia. METHODS: Subjects with severe community-acquired pneumonia (n = 102) were included in the study. Blood samples were collected from each subject 24 h after admission. Data regarding age, sex, PaO2 /FIO2, comorbidities, occurrence of altered mental status, bacteremia, septic shock, Acute Physiology and Chronic Health Evaluation (APACHE) II score, and Sequential Organ Failure Assessment (SOFA) score within the first 24 h; the highest temperature within 24 h after admission; mechanical ventilation usage; timing of antibiotic therapy; ICU stay; and 28-d survival were collected. Expression of mHLA-DR was measured by flow cytometry. RESULTS: APACHE II score and SOFA score were significantly higher (P < .001), whereas the mHLA-DR expression was significantly lower (P < .001) in the non-survivors than in the survivors. The outcomes at day 28 after admission were significantly associated with the APACHE II score (P = .002, odds ratio [OR] = 1.27, 95% CI 1.10-1.48), the SOFA score (P = .003, OR = 1.52, 95% CI 1.15-2.00), and mHLA-DR level (P = .01, OR = 0.91, 95% CI 0.85-0.98), as shown by logistic regression. The area under the receiver operating characteristic curve was 0.877 (95% CI 0.81-0.94, P < .001), 0.862 (95% CI 0.79-0.93, P < .001), and 0.781 (95% CI 0.69-0.87, P < .001) for APACHE II score, SOFA score, and the mHLA-DR expression, respectively. The optimal threshold for mHLA-DR level was 27.2%. Kaplan-Meier survival analysis showed that subjects with mHLA-DR ≥ 27.2% had significantly better outcomes compared with < 27.2% level (P < .001, log rank test, hazard ratio = 0.963, 95% CI 0.94-0.99). CONCLUSIONS: mHLA-DR may be a reliable biomarker that can predict the outcomes of patients with severe community-acquired pneumonia, and 27.2% may be the cut-off value to predict the outcomes.


Assuntos
APACHE , Antígenos HLA-DR/sangue , Monócitos/imunologia , Pneumonia/imunologia , Pneumonia/mortalidade , Adulto , Idoso , Idoso de 80 Anos ou mais , Área Sob a Curva , Biomarcadores/sangue , Infecções Comunitárias Adquiridas/imunologia , Infecções Comunitárias Adquiridas/mortalidade , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Prognóstico , Curva ROC , Índice de Gravidade de Doença , Taxa de Sobrevida
19.
Burns ; 41(6): 1235-41, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26088147

RESUMO

We investigated the effectiveness of ligustrazine (tetramethylpyrazine, TMP) in alleviating pulmonary damage induced by lipopolysaccharide (LPS). Twenty-four healthy male Sprague-Dawley rats were randomly divided into three groups: the blank group, LPS group, and TMP treatment group (TMP group). The LPS group was intraperitoneally injected with LPS (20mg/kg), and the TMP group was intraperitoneally injected with LPS (20mg/kg) and ligustrazine (80mg/kg). Blood gas analysis, hematoxylin-eosin staining dye extravasation and diffuse alveolar damage (DAD) score, the wet/dry lung tissue (W/D) ratios, the expression of CD31+ vascular endothelial microparticles (EMPs), tumor necrosis factor alpha (TNF-α) levels, and the protein expression of Rho-associated coiled-coil-forming protein kinase (ROCK) II and Toll-like receptor 4 (TLR4) were analyzed. Compared with the blank group, the arterial partial pressure of oxygen (PaO2) declined in both 1 and 4h (P<0.01), the W/D ratio and DAD score increased (P<0.01), and the TNF-α levels in serum, CD31+ EMPs, and protein expression of ROCK II and TLR4 were significantly increased (P<0.01) in the LPS group. Compared with the LPS group, PaO2 significantly increased in the TMP group at 4h (P<0.05), while the W/D ratio and DAD score were significantly decreased in the TMP group (P<0.01). TNF-α levels, CD31+ EMPs, and protein expression of ROCK II and TLR4 were significantly decreased in the TMP group compared with the LPS group (P<0.01). This study demonstrated that TMP can alleviate LPS-induced pulmonary damage by attenuating pulmonary vascular permeability and CD31+ EMP levels in the plasma, reducing the release of the inflammatory mediator TNF-α and inhibiting the protein expression of ROCK II and TLR4.


Assuntos
Lipopolissacarídeos/toxicidade , Lesão Pulmonar/induzido quimicamente , Pulmão/efeitos dos fármacos , Oxigênio/sangue , Pirazinas/farmacologia , Sepse/metabolismo , Vasodilatadores/farmacologia , Animais , Gasometria , Micropartículas Derivadas de Células/efeitos dos fármacos , Micropartículas Derivadas de Células/metabolismo , Modelos Animais de Doenças , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/metabolismo , Injeções Intraperitoneais , Pulmão/metabolismo , Lesão Pulmonar/sangue , Lesão Pulmonar/metabolismo , Masculino , Pressão Parcial , Molécula-1 de Adesão Celular Endotelial a Plaquetas/efeitos dos fármacos , Molécula-1 de Adesão Celular Endotelial a Plaquetas/metabolismo , Ratos , Ratos Sprague-Dawley , Receptor 4 Toll-Like/efeitos dos fármacos , Receptor 4 Toll-Like/metabolismo , Fator de Necrose Tumoral alfa/efeitos dos fármacos , Fator de Necrose Tumoral alfa/metabolismo , Quinases Associadas a rho/efeitos dos fármacos , Quinases Associadas a rho/metabolismo
20.
Virus Res ; 188: 38-44, 2014 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-24704672

RESUMO

T-cell responses directed against human cytomegalovirus (HCMV) glycoprotein B (gB) contribute to protective immunity against HCMV infection in both animal models and humans. However, the gB-specific human CD8(+) T cell responses remain poorly understood. gB antigen-specific CD8(+) T cells were stained with seven major histocompatibility complex (MHC)-peptide pentamers in 16 human leukocyte antigen (HLA)-A 1101-positive, HCMV-seropositive patients following hematopoietic stem cell transplantation (HSCT). Of these seven pentamers, the most frequent CD8(+) T-cell responses were directed against the gB332-340 peptide. These gB332-340-specific CD8(+) T cells were strongly associated with the presence of plasma HCMV immunoglobulin M in all HSCT recipients and exhibited a probable causal relationship with the level of pp65 antigenemia. Together, these data suggest a role for gB332-340-specific CD8(+) T cells in HCMV reactivation after HSCT. Furthermore, the pentamer assay may be valuable in detecting antigen-specific CD8(+) T cells.


Assuntos
Linfócitos T CD8-Positivos/imunologia , Antígeno HLA-A11/imunologia , Transplante de Células-Tronco , Transplantados , Transplante Homólogo , Proteínas do Envelope Viral/imunologia , Adolescente , Adulto , Anticorpos Antivirais/sangue , Citomegalovirus/imunologia , Citomegalovirus/fisiologia , Feminino , Humanos , Imunoglobulina M/sangue , Masculino , Pessoa de Meia-Idade , Coloração e Rotulagem , Subpopulações de Linfócitos T/imunologia , Ativação Viral , Adulto Jovem
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