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1.
Breast Cancer ; 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31410735

RESUMO

OBJECTIVE: The miR-638 acted as a tumor suppressor and E2F transcription factor 2 (E2F2) was a critical regulator in some cancers, while the role of them on stemness of breast cancer stem cells (BCSCs) was rarely detailed. Hence, we focused on exploring the effects of miR-638 and E2F2 on BCSCs stemness. METHODS: The proportion of CD24 -/CD44 + cells of BCSCs was detected by flow cytometry. The target relationship of miR-638 and E2F2 was explored using luciferase assays. The ability of self-renewal, proliferation, and invasion of BCSCs were determined by Mammosphere forming, Cell Counting Kit-8 (CCK-8), colony formation, and transwell assays. Xenograft tumor was established to detect the influence of miR-638 on tumor growth. RESULTS: miR-638 was down-regulated, while E2F2 was elevated in breast cancer. The E2F2 level was negatively correlated with miR-638. The BCSCs represented higher proportion of CD24 -/CD44 + cells and levels of sex determining region Y-box 2 (SOX2) and octamer-binding transcription factor 4 (OCT4). The miR-638 was down-regulated and E2F2 was increased in BCSCs. MiR-638 could target to E2F2 and decreased the level of E2F2 in BCSCs cells. Overexpression of miR-638 decreased the proportion of CD24 -/CD44 + cells and the levels of SOX2 and OCT4 by inhibiting E2F2. The overexpression of miR-638 also inhibited the abilities of self-renewal, proliferation, and invasion of BCSCs by inhibiting E2F2. The miR-638 overexpression inhibited the breast tumor growth. CONCLUSION: MiR-638 represses the characteristics and behaviors of BCSCs by targeting E2F2. MiR-638 may be a potential target for breast cancer therapy.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31400186

RESUMO

Stable metal clusters that can resist both highly concentrated acid and alkali are unknown. Herein, we present the first discrete neutral cluster, Hf13(µ4-O)8(OCH3)36 (1), which features extraordinary chemical stability with preserving crystalline state in both concentrated acid (10 M HNO3) and alkali (20 M boiling NaOH) aqueous solution. Importantly, 1 is the first luminescence probe for detecting both concentrated alkali (20 M NaOH) and strong acid (1 M HNO3) with high selectivity and repeatability. Theoretical studies of the electronic structure and bonding with density functional theory reveal that 1 has an extremely large HOMO-LUMO gap due to strong (d-p)π bonding that accounts for the ultrahigh stability. This work enriches functionalized metal clusters and luminescence sensor portfolio, laying the foundation for further construction of stable cluster-based materials.

3.
J Craniofac Surg ; 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31343590

RESUMO

Conjunctival prolapse may occur following ocular, eyelid, and orbital surgeries. Conjunctival prolapse usually results as a complication of maximal levator resection or cosmetic lower eyelid blepharoplasty. Here, we describe conjunctival prolapse as an unexpected complication of frontalis muscle flap transfer for severe ptosis. On postoperative day 5, the patient experienced upper eyelid swelling after closing his eyes suddenly and standing up abruptly. The conjunctiva was reddish and ballooned up, and they protruded over the eyelids. Conjunctival prolapse persisted until postoperative day 8. The patient and surgeon were concerned that this complication would affect ptosis correction and surgical outcome. U-shaped fixations were placed to suture and force the prolapsed conjunctiva back to their normal anatomical positions. At postoperative 6 months, the patient had not experienced additional issues, and he was satisfied with the appearance of his eyes. This report describes a rare clinical case of conjunctival prolapse and provides a reference for surgeons treating similar complications.

4.
Anal Biochem ; 583: 113364, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31323206

RESUMO

Long non-coding RNA (lncRNA) plays an important role in cells through the interaction with RNA-binding proteins (RBPs). Finding the RBPs binding sites on the lncRNA chains can help to understand the post-transcriptional regulatory mechanism, exploring the pathogenesis of cancers and possible roles in other diseases. Although many genome-wide RBP experimental techniques can identify the RNA-protein interactions and detect the binding sites on RNA chains, they are still time-consuming, labor-intensive and cost-heavy. Thus, many computational methods have been developed to predict the RBPs sites by integrating the RNA sequence, structure and domain specific features, etc. However, current approaches that focus on predicting the RBPs binding sites on RNA chains lack a consideration of the dependencies among nucleotides. In this work, we propose a higher-order nucleotide encoding convolutional neural network-based method (namely HOCNNLB) to predict the RBPs binding sites on lncRNA chains. HOCNNLB first employs a high-order one-hot encoding strategy to encode the lncRNA sequences by considering the dependence among nucleotides, then the encoded lncRNA sequences are fed into the convolutional neural network (CNN) to predict the RBP binding sites. We evaluate HOCNNLB on 31 experimental datasets of 12 lncRNA binding proteins. The average AUC of HOCNNLB achieves 0.953, which is 0.247, 0.175 higher than that of iDeepS and DeepBind, respectively. The average accuracy is 90.2%, which is 26.8%, 19.5% higher than that of iDeepS and DeepBind, respectively. These results demonstrate that HOCNNLB can reliably predict the RBP binding sites on lncRNA chains and outperforms the state-of-the-art methods. The source code of HOCNNLB and the datasets used in this work are available at https://github.com/NWPU-903PR/HOCNNLB for academic users.

5.
Inorg Chem ; 58(11): 7433-7439, 2019 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-31117628

RESUMO

Single transition-metal site catalysts with s-, p-, or d-block atom anchor for nitrogen fixation have been extensively studied, and yet the studies of the f-block atom anchor are rarely reported. Thus, we investigate the feasibility of using a newly synthesized U-Co complex featuring a single CoI site coordinated by tetrakis(phophinoamide) and an UIV anchor for N2-to-NH3 conversion by theoretical modeling. We characterize the evolution of oxidation states of U and Co along the reaction pathways from ab initio density matrix renormalization group (DMRG) calculations, and we find that the variation of the Co → U dative bond is correlated with the changes of oxidation states. Both uranium and cobalt can serve as electron reservoirs to facilitate breaking the N-N bond. Our study demonstrates the viability of metal → metal dative bonds, particularly the df-d one, for the reduction of N2 to NH3, and thus, this opens up a new avenue to the rational design of efficient catalyst for nitrogen fixation.

6.
Biomed Environ Sci ; 32(3): 231-233, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30987699

RESUMO

On December 14, 2017, a faculty member of a university in Hunan Province reported that an anthrax vaccine strain might have recovered virulence during an undergraduate experiment and potential exposure could not be ruled out for the students involved. Upon receiving the case report, the CDC, health bureaus, and local governments at the county, prefectural, and provincial levels promptly organized experts in different fields (including epidemiologists, biosafety experts, and laboratory testing experts) for case investigation, evaluation, and response. As the investigation results showed, no virulence recovery was identified in the involved anthrax vaccine strain; and no contamination of Bacillus anthracis was detected at the involved areas. Thus, the university returned to normal functioning.


Assuntos
Vacinas contra Antraz/análise , Bacillus anthracis/patogenicidade , Contenção de Riscos Biológicos , China , Humanos , Laboratórios/estatística & dados numéricos , Virulência
7.
Chem Commun (Camb) ; 55(40): 5685-5688, 2019 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-31020278

RESUMO

Heteronuclear transition-metal-main-group element carbonyl anion complexes of AFe(CO)3- (A = Ge, Sn, and Pb) are prepared using a laser vaporization supersonic ion source in the gas phase, which were studied by mass-selected infrared (IR) photodissociation spectroscopy. The geometric and electronic structures of the experimentally observed species are identified by a comparison of the measured and calculated IR spectra. These anion complexes have a 2A1 doublet electronic ground state and feature an A[triple bond, length as m-dash]Fe triply bonded C3v structure with all of the carbonyl ligands bonded at the iron center. Bonding analyses of AFe(CO)3- (A = C, Si, Ge, Sn, Pb, and Fl) indicate that the complexes are triply bonded between the valence np atomic orbitals of bare group-14 atoms and the hybridized 3d and 4p atomic orbitals of iron.

8.
Toxicol Lett ; 309: 1-9, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30904571

RESUMO

Aflatoxin M1 (AFM1), ochratoxin A (OTA), and zearalenone (ZEA) are mycotoxins commonly found in milk. Mycotoxin contamination has caused food safety concerns worldwide since most of the toxic effects in humans are serious. The combined toxic effects of these mycotoxins on intestinal epithelial cells have not been reported. Herein, we investigated the combined effects of AFM1, OTA, and ZEA on intestinal integrity and define the underlying mechanisms(s) of their effects in Caco-2/HT29-MTX co-cultures. Our results showed that the mixtures of AFM1 + OTA, AFM1 + ZEA, and AFM1 + ZEA + OTA significantly increased epithelial permeability. Immunofluorescence analysis and transmission electron microscopy revealed that mycotoxins altered TJ proteins morphology and disrupted their structures. Also, the present study showed that mixtures of mycotoxins significantly modulated MUC5AC and MUC5B mRNA levels and protein secretion. This study demonstrated that the effects of mixtures of mycotoxins on intestinal barrier function were more significant than AFM1 alone. More importantly, the damage of intestinal integrity caused by mycotoxins was correlated to the change of the TJ proteins location and the decrease of mucin secretion. Mixtures of AFM1, OTA, and ZEA in food might pose a health risk to consumers, particularly in children, and toxin risks should be considered.


Assuntos
Aflatoxina M1/toxicidade , Mucosa Intestinal/efeitos dos fármacos , Mucinas/metabolismo , Ocratoxinas/toxicidade , Zearalenona/toxicidade , Células CACO-2 , Técnicas de Cocultura , Contaminação de Alimentos , Células HT29 , Humanos , Mucosa Intestinal/metabolismo , Mucina-5AC/análise , Mucina-5AC/genética , Mucina-2/análise , Mucina-2/genética , Mucina-5B/análise , Mucina-5B/genética , Mucinas/genética , Permeabilidade , RNA Mensageiro/análise , Proteínas de Junções Íntimas/análise
9.
Chin Med J (Engl) ; 132(11): 1314-1321, 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-30888986

RESUMO

BACKGROUND: Fusion genes may play an important role in tumorigenesis, prognosis, and drug resistance; however, studies on fusion genes in endometrial cancer (EC) are rare. This study aimed to identify new fusion genes and to explore their clinical significance in EC. METHODS: A total of 28 patients diagnosed with EC were enrolled in this study. RNA sequencing was used to obtain entire genomes and transcriptomes. STAR-comparison and STAR-fusion prediction were applied to predict the fusion genes. Chi-square tests and Student t tests were used to verify the clinical significance with SPSS 13.0 software. RESULTS: New fusion genes were found, and the number of fusion genes varied from 3 to 110 among all patients with EC. The type of fusion genes varied and included messenger RNA (mRNA)-mRNA, long non-coding RNA (lncRNA)-lncRNA, and lncRNA-mRNA. There were six fusion genes with high fusion rates, namely, RP11-123O10.4-GRIP1, RP11-444D3.1-SOX5, RP11-680G10.1-GSE1, NRIP1-AF127936.7, RP11-96H19.1-RP11-446N19.1, and DPH7-PTP4A3. Further studies showed that these fusion genes are related to stage, grade, and recurrence, in which NRIP1-AF127936.7 and DPH7-PTP4A3 were found only in stage III patients with EC. DPH7-PTP4A3 was found in grades 2 and 3, and recurrent patients with EC. CONCLUSION: Fusion genes play an essential role in EC. Six genes that are overexpressed with high fusion rates are identified. NRIP1-AF127936.7 and DPH7-PTP4A3 might be related to stage, and DPH7-PTP4A3 be related to grade and recurrence.

10.
Inorg Chem ; 58(6): 3670-3675, 2019 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-30817144

RESUMO

A pair of enantiopure Au13 nanoclusters have been enantioselectively synthesized by chiral ligands with stereogenic centers at the phosphorus atoms. Their structures are determined by X-ray crystallography, which are typical models with a high symmetric core and chiral surface ligand arrangement. Correlation between the crystallographic structure, the calculation, and the circular dichroism (CD) study indicates that helical ligand arrangement inducing the core into chiral distortion accounts for the chiroptical activities in the visible region. A rare example of cocrystallization of a mixture of diastereomers has been observed for the first time for gold nanoclusters, reflecting the lack of chiral self-sorting of the ligands.

11.
Ying Yong Sheng Tai Xue Bao ; 30(2): 644-652, 2019 Feb 20.
Artigo em Chinês | MEDLINE | ID: mdl-30915817

RESUMO

Yangtze River Estuary is the biggest estuarine ecosystem in the western Pacific Ocean. Evaluating fish community in this ecosystem can provide scientific basis for its restoration and mana-gement. Generalized additive model (GAM) and boosted regression tree (BRT) were built to examine the relationship between fish community diversity and environmental and spatio-temporal variables based on data collected during 2012-2014. Combined with linear regression analysis, a cross validation was used to evaluate the fitness and predictive performance of both models. We plotted the spatial distribution of fish community diversity and richness in each station of the Yangtze River Estuary in 2014. The results showed that salinity, pH and chlorophyll-a had the most contribution on diversity, while pH, dissolved oxygen and chlorophyll-a were the most contributive variables on richness. BRT models showed better fitness and lower prediction error than GAM models. In contrast to GAM models, BRT models could distinguish the fish community index in each station area with respect to the spatial prediction. The diversity index in external water was obviously greater than that in internal water. Meanwhile, the station at higher latitude had a higher diversity index in both external and internal water.


Assuntos
Rios , Animais , China , Estuários , Peixes , Oceano Pacífico
12.
Opt Lett ; 44(5): 1150-1153, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30821735

RESUMO

To extend the coherence of quantum transitions for laser locking, as well as increase the compactness and stability of the experimental setup, we propose to utilize photonic integrated resonators with high second-harmonic (SH) generation efficiencies as reliable frequency doublers that link the desired frequencies with the frequency references. In this Letter, a sufficiently strong SH signal up to microwatts was generated by a photonic integrated frequency doubler using a milliwatt infrared (IR) laser source. Furthermore, an increased SH generation bandwidth covering Rb85 and Rb87D2 transition lines, as well as saturated absorption spectroscopy, was demonstrated by tuning the pump power and chip temperature. Here we present, to the best of our knowledge, the first successful locking of an IR laser to Rb saturated absorption lines via a photonic chip frequency doubler.

13.
J Am Chem Soc ; 141(6): 2384-2390, 2019 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-30657319

RESUMO

We report the synthesis and crystal structure determination of two novel chiral gold nanoclusters. By utilizing BINAP, we isolate atomically precise intrinsically chiral Au nanoclusters [Au9( R-/ S-BINAP)4](CF3COO)3 and [Au10( R-/ S-BINAP)4( p-CF3C6H4C≡C)](CF3COO)3 in high yield with one-pot synthesis. Au9 has C2 geometry, while Au10 is C1 symmetric. Interestingly, reversible interconversion between Au9 and Au10 can be realized by the addition or removal of a RC≡CAu component. The transformation from Au9 to Au10 leads to significant enhancement of CD signals. The maximum anisotropy factor in the visible region of [Au10(BINAP)4( p-CF3C6H4C≡C)]3+ is the hitherto largest (up to 6.6 × 10-3) among gold nanoclusters, which is approximately twice of that of [Au9(BINAP)4]3+. This work demonstrates that the chiroptical activity of gold nanoclusters can be modulated by structural control through the introduction of second ligands.

14.
J Agric Food Chem ; 2018 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-30418775

RESUMO

To investigate the effect of heat treatment on the antitumor activity of lactoferrin in colon cancer cells and colon tumors, the HT-29 (human intestinal epithelial tumor cell) cell line was exposed to lactoferrin and various heat treatments. The impacts on cell proliferation, invasion, and migration were observed in vitro, and nude mice bearing HT29 tumors were administered lactoferrin and underwent various heat treatments in vivo. In the HT29 cell proliferation test using transwell and scratch analyses, lactoferrin (20 mg/mL) without or with heat treatment (50 and 70 °C) significantly inhibited cell proliferation, migration, and invasion (compared with the control, p < 0.05), while lactoferrin with heat treatment (100 °C) did not affect these parameters. In vivo, HT29 tumor weight was significantly reduced in the lactoferrin (without heat treatment and with 50 and 70 °C treatment) groups (1.59 ± 0.20, 1.67 ± 0.25, and 2.41 ± 0.42 g, compared with the control, p < 0.05), and there was no significant difference between the control (3.73 ± 0.33 g) and the 100 °C treatment group (3.58 ± 0.29 g). Moreover, 100 °C heat treatment reduced inhibition of the VEGFR2/VEGFA/PI3K/Akt/Erk1/2 angiogenesis pathway by lactoferrin. In summary, HT29 tumors were effectively suppressed by lactoferrin via inhibition of VEGFR2/VEGFA/PI3K/Akt/Erk1/2 pathway, and heat treatment affected the antitumor activity of lactoferrin in a temperature-dependent manner.

15.
J Am Chem Soc ; 140(46): 15842-15849, 2018 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-30380854

RESUMO

In mammals, 5-formylcytosine (5fC) has been identified as an important mark, which plays significant roles in active DNA demethylation and also in epigenetic regulation. It is therefore important to target this epigenetic mark as well as manipulating DNA-protein interactions at this site. A unique feature of 5fC is the presence of a formyl group at the C-5 position. In the current study, we introduce supramolecular coordination chemistry for reversible regulation of DNA-protein interactions on this mark. We have designed and synthesized the 2-(aminooxy)- N-(quinolin-8-yl)acetamide (AQA), which functions well in selective labeling of 5fC mark. Using this feature, the association and disassociation of metal ion supplementation allow blocking and deblocking of DNA-protein interactions. In addition, we synthesized a close analogue of AQA by replacing the nitrogen atom in the quinoline ring with a CH group. Importantly, the regulatory effects of those metal ion supplementations were completely erased. On the basis of the combined information, we propose a conformational flexibility in a side arm in response to switched coordination. In the absence of coordinating interaction, the flexible side arm probably takes on an extended conformation and points away from the hydrogen bonding cavity. Importantly, coordinating interaction is effective in imposing a restrained geometry to this side arm, with the quinoline ring being oriented opposite the complementary nucleobase. Moreover, the coordination-induced activity control can be reversed by supplementation with a number of chelating agents. The concept described is unique in installing an auxiliary side arm with bending flexibility to control oligonucleotide functions. Finally, these findings show promising potential of supramolecular coordination chemistry for DNA epigenetics.

16.
J Org Chem ; 83(19): 11532-11540, 2018 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-30168321

RESUMO

A route to enantiopure ( O-methyl)6-2,6-helic[6]arenes (+)- P-2 and (-)- M-2 has been provided. By the reaction of enantiopure triptycene precursors (+)-1 and (-)-1 in refluxed o-DCB for 12 h in the presence of catalytic amount of FeCl3, and then followed by treatment of the obtained oligomers under the same conditions, (+)- P-2 and (-)- M-2 could be obtained in 51% and 53% total yield, respectively. It was also found that racemic and enantiopure ( O-methyl)6-2,6-helic[6]arenes could be easily brominated by Br2 to give the corresponding hexabromo-substituted helic[6]arene derivatives rac-4, (+)- P-4, and (-)- M-4 in high yields. The crystal structure of (+)- P-4 further confirmed the absolute configuration of the helic[6]arenes and their derivatives. Moreover, a series of hexaaryl-substituted helic[6]arene derivatives 5a-f with deepened cavities could be conveniently synthesized in 55-71% yield by Suzuki-Miyaura coupling reactions of 4 and arylboronic acids. rac-5a could encapsulate chloroform and exhibit self-sorting stacking in solid state. Enantiopure (+)- P-5a-f and (-)- M-5a-f showed mirror images in their CD spectra.

17.
Adv Sci (Weinh) ; 5(7): 1800231, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30027051

RESUMO

As a left-handed helical structure, Z-DNA is biologically active and it may be correlated with transcription and genome stability. Until recently, it remained a significant challenge to control the B/Z-DNA transition under physiological conditions. The current study represents the first to reversibly control B/Z-DNA transition using cucurbit[7]uril-based supramolecular approach. It is demonstrated that cucurbit[7]uril can encapsulate the central butanediamine moiety [HN(CH2)4NH] and reverses Z-DNA caused by spermine back to B-DNA. The subsequent treatment with 1-adamantanamine disassembles the cucurbit[7]uril/spermine complex and readily induces reconversion of B- into Z-DNA. The DNA conformational change is unequivocally demonstrated using different independent methods. Direct evidence for supramolecular interactions involved in DNA conformational changes is further provided. These findings can therefore open a new route to control DNA helical structure in a reversible way.

18.
J Craniofac Surg ; 29(6): 1558-1561, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29863552

RESUMO

Forehead aging is characterized by wrinkles, loss of skin elasticity, brow ptosis, and soft-tissue atrophy. For patients with prominent rhytids and marked brow ptosis, forehead lift is still the most effective treatment with a persisting result. In order to eliminate the glabellar wrinkles, forehead lift usually requires the removal of the corrugator supercilii muscle and procerus, which can lead to glabellar flattening or depression. Instead of muscle removal, the corrugator supercilii muscle, procerus, and the underlying galea were dissected as a pedicled glabellar flap. Then reversed periosteum or dermal fat graft was used to cover the glabellar flap to restore the glabellar volume. From January 2005 to November 2014, a total of 164 coronal and 42 trichophytic forehead lifts were performed. Reversed periosteum was used to cover the glabellar flap in 191 patients while dermal fat graft was applied in 15 patients with a follow-up period ranging from 6 months to 10 years. There was no irregularity or depression in the glabellar region in the group of reversed periosteal flap. The take of dermal fat graft placed over the glabellar flap was minimal. Complications from surgical procedures occurred in 2.91% of the patients. There was 1 asymmetry, 4 patients with higher than desired frontal hairline for implantation of autologous follicular units, and 1 patient with scar hyperplasia. There was no hematoma or nerve injury, no permanent numbness, and no alopecia. The techniques are simple and effective to eliminate the glabellar wrinkles and maintain or restore the glabellar volume.

19.
J Phys Chem A ; 122(18): 4530-4537, 2018 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-29648830

RESUMO

Model systems of the FeMo cofactor of nitrogenase have been explored extensively in catalysis to gain insights into their ability for nitrogen fixation that is of vital importance to the human society. Here we investigate the trigonal pyramidal borane-ligand Fe complex by first-principles calculations, and find that the variation of oxidation state of Fe along the reaction path correlates with that of the reverse-dative Fe → B bonding. The redox-flexibility of the reverse-dative Fe → B bonding helps to provide an electron reservoir that buffers and stabilizes the evolution of Fe oxidation state, which is essential for forming the key intermediates of N2 activation. Our work provides insights for understanding and optimizing homogeneous and surface single-atom catalysts with reverse-dative donating ligands for efficient dinitrogen fixation. The extension of this kind of molecular catalytic active center to heterogeneous catalysts with surface single-clusters is also discussed.

20.
Toxicol Lett ; 291: 101-111, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29458171

RESUMO

AIM: Furosine is one of the Maillard reaction products (MRPs) and is found in a variety of heat-processed food. Yet its toxicity is still unclear. The present study was designed to assess furosine toxicity in cell models and in CD-1 mice, respectively. METHODS: In vitro, the effects of furosine on the cell viability, cell cycle and apoptosis (Hek293, HepG2, SK-N-SH and Caco2) were detected and evaluated, sensitive cell lines and proper dosage of furosine for further animal experiment were determined, and the mechanisms of toxicity were explored. In vivo, the acute toxicity studieswere performed, organ index, hematology parameters, functions of liver/kidney and pathological changes were detected and the target organs were uncovered. RESULTS: Hek293 cells and HepG2 cells were themost sensitive to furosine with respect to cytotoxicity and apoptosis. Furosine inhibited mice weight gain, and affected the functions of liver and kidney. CONCLUSIONS: Furosine posed toxic effects on mice liver and kidney, suggested thatthey were the target organs for furosine toxicity. This study for the first time provides evidence that high dosages of furosine pose adverse biological effects on the health of animals through induction of cell apoptosis and activation of inflammatory necrosis response.


Assuntos
Lisina/análogos & derivados , Animais , Apoptose/efeitos dos fármacos , Contagem de Células Sanguíneas , Células CACO-2 , Ciclo Celular/efeitos dos fármacos , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Feminino , Células HEK293 , Humanos , Testes de Função Renal , Testes de Função Hepática , Lisina/toxicidade , Masculino , Camundongos , Necrose , Ganho de Peso/efeitos dos fármacos
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