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1.
J Cell Mol Med ; 2020 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-32108988

RESUMO

The GNPAT variant rs11558492 (p.D519G) was identified as a novel genetic factor that modifies the iron-overload phenotype in homozygous carriers of the HFE p.C282Y variant. However, the reported effects of the GNPAT p.D519G variant vary among study populations. Here, we investigated the role of GNPAT in iron metabolism using Gnpat-knockout (Gnpat-/- ), Gnpat/Hfe double-knockout (Gnpat-/- Hfe-/- or DKO) mice and hepatocyte-specific Gnpat-knockout mice (Gnpatfl/fl ;Alb-Cre). Our analysis revealed no significant difference between wild-type (Gnpat+/+ ) and Gnpat-/- mice, between Hfe-/- and DKO mice, or between Gnpatfl/fl and Gnpatfl/fl ;Alb-Cre with respect to serum iron and tissue iron. In addition, the expression of hepcidin was not affected by deleting Gnpat expression in the presence or absence of Hfe. Feeding Gnpat-/- and DKO mice a high-iron diet had no effect on tissue iron levels compared with wild-type and Hfe-/- mice, respectively. Gnpat knockdown in primary hepatocytes from wild-type or Hfe-/- mice did not alter hepcidin expression, but it repressed BMP6-induced hepcidin expression. Taken together, these results support the hypothesis that deleting Gnpat expression has no effect on either systemic iron metabolism or the iron-overload phenotype that develops in Hfe-/- mice, suggesting that GNPAT does not directly mediate iron homeostasis under normal or high-iron dietary conditions.

2.
Int J Biol Macromol ; 150: 573-577, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32057877

RESUMO

Lentinula edodes polysaccharides from at four different development stages (referred to L1, L2, L3 and L4, respectively) were extracted by hot water method, and graded ethanol precipitation to final concentration of 20%, 50% and 70%, then12 crude polysaccharide fractions (referred to L1P20, L2P20, L3P20; L4P20, L1P50, L2P50, L3P50, L4P50 and L1P70, L2P70, L3P70, L4P70, respectively) were obtained. Physicochemical properties and exoteric bioactivities of the crude polysaccharide fractions were measured. The results of physicochemical properties revealed that extraction yields of P20 fractions were significantly higher than those of P50 and P70 fractions, and the contents of polysaccharide and ß-glucan in L3P50 fractions were higher, and the viscosity-average molecular weight reached a maximum at L2, and high molecular weight polysaccharides could be obtained at a low alcohol concentration in P20 fractions, and the glycosidic bonds were found to exist in all crude polysaccharide fractions. These crude polysaccharide fractions showed different bioactivities, wherein the polysaccharides of higher molecular weight in P20 fractions had greater bioactivity. These results showed that immature stage of Lentinula edodes was the optimal harvest time for obtaining higher bioactivity of crude polysaccharides.

3.
Nat Prod Res ; : 1-10, 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32037886

RESUMO

Sanguisorba officinalis L. is a traditional herbal plant that belongs to the genus Sanguisorba and the family Rosaceae. Two new phenolic glycosides (1-2), ten known phenolics (3-12), and six known monoterpenoid glycosides (13-18) were isolated from the roots of S. officinalis using silica gel column and preparative middle pressure liquid chromatography (MPLC). The chemical structures were elucidated based on extensive spectroscopic experiments, including 1D and 2D NMR as well as HR-ESI-MS, and comparison with those reported in the literature. Compounds 3-5, and 13 were isolated from the Rosaceae family and compound 7 was obtained from the genus Sanguisorba for the first time. Additionally, all compounds were evaluated for their anti-complementary activities against the classical pathway. Furthermore, compounds 1, 5, 9, and 14 showed significant anti-complementary activities with the 50% haemolytic inhibition concentrations (CH50) values of 0.40 ± 0.03, 0.57 ± 0.01, 0.51 ± 0.07, and 0.53 ± 0.05 mM, respectively.

4.
Breast Cancer ; 2020 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-31927711

RESUMO

Radiation-induced sarcoma (RIS) is a late complication of breast cancer radiotherapy, with a very low incidence, long latent period and poor prognosis. Among them, leiomyosarcoma after radiation is an extremely rare radiation-associated sarcoma. In this study, we report a case of radiation-induced leiomyosarcoma 2 years after postoperative radiotherapy for breast cancer. After the diagnosis of leiomyosarcoma, the patient received radical resection of the left chest wall leiomyosarcoma. The patient showed no relapse or progression during the follow-up time of 1 year after operation. Rare occurrence of RIS induced by postoperative radiotherapy in breast cancer and limited experience concerning its diagnosis and treatment bring obstacle to both patients and doctors. Preoperative examinations must be carefully refined. With a perfect coordination between different disciplines, RIS induced by postoperative radiotherapy in breast cancer can be ideally treated with better prognosis.

5.
Nat Prod Res ; 34(2): 225-232, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30580619

RESUMO

One new aryldihydronaphthalene-type lignan (1) together with eight known lignans (2-4, 7-11) as well as two caffeic-acid dimers (5, 6) were isolated from an ethanol extract of the whole plant of Corispermum mongolicum Iljin (Chenopodiaceae). The chemical structures of these compounds were determined from 1D and 2D NMR and HR-ESI-MS spectra, and results were compared with data from the literature. This study is the first demonstration of nine compounds (2 and 4-11) isolated from the Chenopodiaceae family, with one of these (3) from the genus Corispermum. Anti-inflammatory effects of the isolated compounds were evaluated in terms of inhibition of production of nitric oxide, tumour necrosis factor-α, and interleukin-6 in lipopolysaccharide-induced RAW 264.7 cells.

6.
J Hazard Mater ; : 121729, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31787400

RESUMO

A series of ceria-zirconia solid solutions (CexZr1-xO2) were prepared by co-precipitation method and then sulfated with SO2 + O2 at 200 °C. Subsequent testing with the selective catalytic reduction of NO by NH3 (NH3-SCR) showed that the activity of the sulfated CexZr1-xO2 catalysts oxide catalysts exhibited a volcano-type tendency with increasing Zr content. Furthermore, the sulfated Ce0.6Zr0.4O2 catalyst showed the most desirable NH3-SCR activity at 250-300 °C, and exhibited much better SO2 resistance at 250 °C. Detailed characterization results demonstrated that Ce0.6Zr0.4O2 could adsorb more surface sulfate species and then produce more stable acid sites than pure CeO2 at 200 °C. After sulfation treatment, more Ce3+ and oxygen vacancies were formed on the surface of Ce0.6Zr0.4O2. In situ diffuse reflectance infrared Fourier transform spectroscopy (in situ DRIFTS) experiments suggested that the nitrates species deposited on the surface of as-prepared Ce0.6Zr0.4O2, which showed no reactivity, could barely deposit on the same sample after sulfation. While, the sulfated Ce0.6Zr0.4O2 had more reactive acid sites to participate in the NH3-SCR and the reaction proceeded via Eley-Rideal mechanism. This work proved that sulfation treatment could be used in designing an efficient cerium-zirconium based NH3-SCR catalyst with great application prospect.

7.
Ann Surg ; 2019 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-31855875

RESUMO

OBJECTIVE: To determine the prognostic impact of pathologic lymph node (LN) status and investigate risk factors of recurrence in esophageal squamous cell carcinoma (ESCC) patients with pathologic complete response (pCR) after neoadjuvant chemoradiotherapy (NCRT). SUMMARY BACKGROUND DATA: There are no large-scale prospective study data regarding ypN status and recurrence after pCR in ESCC patients receiving NCRT. METHODS: The NEOCRTEC5010 trial was a prospective multicenter trial that compared the survival and safety of NCRT plus surgery (S) with S in patients with locally advanced ESCC. The relationships between survival and cN, pN, and ypN status were assessed. Potential prognostic factors in patients with ypN+ and pCR were identified. RESULTS: A total of 389 ESCC patients (NCRT: 182; S: 207) were included. Patients with pN+ in the S group and ypN+ in the NCRT group had decreased overall survival (OS) and disease-free survival (DFS) compared with pN0 and ypN0 patients, respectively. Partial response at the primary site [hazard ratio (HR), 2.09] and stable disease in the LNs (HR, 3.26) were independent risk factors for lower DFS, but not OS. For patients with pCR, the recurrence rate was 13.9%. Patients with distant LN metastasis had a median OS and DFS of 16.1 months and 14.4 months, respectively. Failure to achieve the median total dose of chemotherapy was a significant risk factor of recurrence and metastasis after pCR (HR, 44.27). CONCLUSIONS: Persistent pathologic LN metastasis after NCRT is a strong poor prognostic factor in ESCC. Additionally, pCR does not guarantee a cure; patients with pCR should undergo an active strategy of surveillance and adjuvant therapy.

8.
Zhongguo Zhong Yao Za Zhi ; 44(17): 3830-3836, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31602960

RESUMO

The peeled root,stem or twig of Syringa pinnatifolia is a representative Mongolian folk medicine with the effects of antidepression and pain relief. It has been used for the treatments of heart tingling,heart palpitations,upset,insomnia and other symptoms. Inspired by Mongolian medical theory and clinical practices,this study evaluated the analgesic effect of S. pinnatifolia ethanol extract( T) through three analgesic models including acetic acid writhing test,formalin test,and hot plate test,and the sedative effect of T was evaluated by locomotor activity and synergistic sleeping experiments,and furthermore the effects of T on the GABAergic nervous system were investigated by ELISA,immunohistochemistry,Western blot,and PCR methods. The results showed that T can significantly reduce the number of writhing,the time of paw licking and extend the thermal threshold of mice,suggesting the analgesic effect of T.T also can indicate its sedative effect by reducing the number of activities,decreasing latency of sleeping and extending sleeping time of mice. ELISA results showed that T can increase the content of GABA/Glu in rat cortex,hippocampus,and hypothalamus,and the most significant increase in hypothalamus. The immunohistochemistry and Western blot results showed that T can up-regulate the expression of GAD67 protein in hypothalamus,and the PCR results showed that T can up-regulate the expression of GABAA Rα1,α2,α3,α5,ß1-3,γ1-3 genes,suggesting a sedative effect through the GABAergic nervous system. In conclusion,this study shed insight into the theoretical basis and clinical application of S. pinnatifolia,and also provides inspiration for subsequent development and application.


Assuntos
Analgésicos/farmacologia , Hipnóticos e Sedativos/farmacologia , Extratos Vegetais/farmacologia , Syringa/química , Animais , Medicina Tradicional da Mongólia , Camundongos , Dor , Ratos
10.
Nat Prod Res ; : 1-9, 2019 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-31385536

RESUMO

Two new flavonoids, (2S)-6,8-dimethyl-5,7,3',4'-tetrahydroxyflavanone 4'-O-ß-D-glucopyranoside (1) and quercetin 3-O-ß-D-(6''-p-methoxybenzoyl)-galactopyranoside (2), together with ten known flavonoids (3-12) were isolated from the leaves of Rhododendron dauricum L. The structures of the flavonoids were characterized from spectroscopic data (1D and 2D NMR and HR-ESI-MS). The isolated flavonoids were evaluated for their inhibitory effects on the production of tumour necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 cells. Compound 11 exhibited inhibitory activity against TNF-α production with an IC50 value of 46.2 ± 1.2 µM.

11.
Nanoscale ; 11(28): 13567-13575, 2019 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-31290895

RESUMO

Two-dimensional (2D) valleytronic systems can provide information storage and processing advantages that complement or surpass those of conventional charge and spin-based semiconductor technologies. For efficient use of the valley degree of freedom, the major challenge currently is to lift the valley degeneracy to achieve valley splitting for further valleytronic operations. In this work, we demonstrate that valley splitting and efficient hole-doping in monolayer WS2 can be achieved by the proximity coupling effect of 2D ferromagnetic MnO2 using density functional theory and Berry curvature calculations. A valley splitting of 43 meV is induced in the valence band of WS2. The efficient hole-doping moves the Fermi level just located between the valence band maxima of the K and K' valleys, which is suitable for the valley-polarized transport. The magnitude of valley splitting relies on the strength of interfacial orbital hybridization and can be tuned continually by applying interfacial compression or an electric field. Owing to the sizable Berry curvature and time-reversal symmetry breaking of WS2, a spin- and valley-polarized anomalous Hall current can be generated. Then, we proposed a valleytronic device that can be used as a filter for both the spin and valley based on this WS2/MnO2 van der Waals heterostructure.

12.
Am J Otolaryngol ; 40(4): 547-554, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31036418

RESUMO

OBJECTIVE: To investigate the genetic and epigenetic differences between human papillomavirus (HPV)-positive oropharyngeal squamous cell carcinoma (OPSCC) and HPV-negative OPSCC. METHODS: Microarray data of HPV-positive and -negative OPSCC were retrieved from NCBI GEO datasets. Differentially expressed genes (DEGs) and differentially expressed miRNAs (DE-miRNAs) were identified by performing differential expression analysis. A functional enrichment analysis was performed to explore the biological processes and signaling pathways that DEGs and DE-miRNAs were involved in, respectively. A protein-protein interaction (PPI) network of DEGs was constructed to identify hub genes. miRNA-target network and miRNA-miRNA functional synergistic network were each constructed in order to identify risk-marker miRNAs. An miRNA-target-pathway network was constructed in order to explore the function of identified risk-marker miRNAs. RESULTS: Microarray data from 3 datasets (GSE39366, GSE40774, and GSE55550) was included and analyzed. The PPI network identified 3 hub genes (VCAM1, UBD, and RPA2). MiR-107 and miR-142-3p were found to play the most significant role in both the DE-miRNA-target network as well as in the miRNA-miRNA functional synergistic network. MiR-107 was involved in HPV-induced tumorigenesis by targeting many genes (CAV1, CDK6, MYB, and SERPINB5) and regulating the p53 signaling pathway, the PI3K-Akt signaling pathway, and the autophagy pathway. In addition, miR-142-3p was implicated in HPV-induced tumorigenesis by targeting the PPFIA1 gene and regulating transcriptional dysregulation and other cancerous pathways. CONCLUSION: Three genes (VCAM1, UBD, and RPA2), two miRNAs (miR-107 and miR-142-3p), and four pathways (p53, PI3K-Akt, autophagy, and transcription dysregulation in cancer) were identified to play critical roles in distinguishing HPV-positive OPSCC from HPV-negative OPSCC.


Assuntos
Carcinogênese/genética , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/virologia , Biologia Computacional , Epigênese Genética/genética , Expressão Gênica , Neoplasias Orofaríngeas/genética , Neoplasias Orofaríngeas/virologia , Papillomaviridae , Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Conjuntos de Dados como Assunto , Humanos , MicroRNAs/genética , MicroRNAs/metabolismo , Análise em Microsséries , Mapas de Interação de Proteínas , Proteína de Replicação A/genética , Proteína de Replicação A/metabolismo , Ubiquitinas/genética , Ubiquitinas/metabolismo , Molécula 1 de Adesão de Célula Vascular/genética , Molécula 1 de Adesão de Célula Vascular/metabolismo
13.
Phys Chem Chem Phys ; 21(20): 10706-10715, 2019 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-31086862

RESUMO

Two-dimensional (2D) materials with intrinsic magnetism and low hole injection barriers to transition metal dichalcogenides are crucial to develop dopant-free all-2D p-type spin field effect transistors for CMOS logic and spintronic applications. Here, the electronic structures of 2D MoS2/MnO2 heterostructures are investigated by first-principles calculations, where the monolayered MnO2 has two polymorphs including magnetic metal h-MnO2 and magnetic semiconductor t-MnO2. Both the MoS2/h-MnO2 and MoS2/t-MnO2 heterostructures show p-type doping for MoS2. In the MoS2/h-MnO2 model with a semiconductor/metal contact, the charge transfer can affect the occupation of Mn 3d and O 2p orbitals, which results in a half-metallic characteristic of the heterostructure with a Schottky barrier height of only 0.15 eV. However, the MoS2/t-MnO2 model with a semiconductor/semiconductor contact shows a spin-gapless electronic structure. Moreover, the type-II band alignment of the MoS2/t-MnO2 heterostructure can facilitate the effective separation of electrons and holes, which can enhance the lifetime of interlayer excitons. The long interlayer exciton lifetime makes it a good candidate for electron-hole separators and related optoelectronic devices. By applying vertical compression, the spin channel of the half-metallic MoS2/h-MnO2 heterostructure can be reversed and the spin-gapless band structure of the MoS2/t-MnO2 heterostructure becomes half-metallic. Furthermore, by applying a gate voltage, the Schottky barrier height and the spin-gapless gap can be tailored. The tunable spin polarization, spin-polarized direction and exciton recombination rate provide a feasible way toward spintronics and optoelectronics.

14.
Food Chem ; 287: 186-196, 2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-30857688

RESUMO

Freezing-thawing events contribute to the unique aroma profile of icewines. Differences in key odor-active volatile compounds between 'Beibinghong' (Vitis amurensis × V. vinifera) icewines and dry wines were investigated by gas chromatography-olfactometry and gas chromatography-mass spectrometry. Acceptable agreement between the olfactometric and quantitative results was obtained. 'Beibinghong' icewine was characterized by high concentrations of volatile phenols, lactones, (E)-ß-damascenone, and phenylacetaldehyde, which were associated with on-vine freezing-thawing events in grape. Low concentrations of higher alcohol acetates and ethyl esters of fatty acids were attributed to hyperosmotic stress during fermentation. The overall aroma of icewine could be mimicked by reconstitution containing 44 identified volatiles. Partial least squares regression analysis demonstrated that the concentrations of these volatile compounds determined the distinct sensory profiles of icewines, which have higher intensities of honey/sweet, smoky, caramel, dried fruit, apricot/peach, and floral aromas, and lower intensities of fresh fruity and herbaceous notes in comparison with dry wines.


Assuntos
Cromatografia Gasosa-Espectrometria de Massas/métodos , Odorantes/análise , Olfatometria , Vinho/análise , Ésteres , Fermentação , Manipulação de Alimentos/métodos , Congelamento , Frutas/química , Humanos , Análise dos Mínimos Quadrados , Norisoprenoides , Paladar , Vitis/química , Compostos Orgânicos Voláteis/análise
15.
Nat Prod Res ; : 1-7, 2019 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-30810367

RESUMO

A new pentacyclic triterpenoid, 3-oxo-urs-11,13(18)-dien-28-oic acid (1), along with twelve known triterpenoids, α-amyrin (2), 19α-hydroxy-α-amyrin (3), triptohypol E (4), uvaol (5), 2α,3α-dihydroxyurs-11-en-13ß,28-olide (6), 3ß-hydroxyurs-11-en-13ß,28-olide (7), ursolic acid (8), asiatic acid (9), oleanolic acid (10), aegiceradienol (11), obtusalin (12) and betulinic acid (13) were isolated from the leaves of Rhododendron dauricum L. Their structures were established from spectroscopic data and comparison with reported values. Among them, compounds 3, 4, 6, 7 and 11 were isolated from the Ericaceae family for the first time. Compounds 2, 5, 9, 12 and 13 were obtained from R. dauricum for the first time. Additionally, compounds 6, 10 and 11 significantly inhibited the levels of NO in LPS-stimulated RAW 264.7 cells at 3 µM.

16.
ACS Appl Mater Interfaces ; 11(5): 4900-4907, 2019 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-30650968

RESUMO

Sulfur poisoning has long been recognized as a bottleneck for the development of long-lived NH3-selective catalytic reduction (SCR) catalysts. Ammonium bisulfate (ABS) deposition on active sites is the major cause of sulfur poisoning at low temperatures, and activating ABS decomposition is regarded as the ultimate way to alleviate sulfur poisoning. In the present study, we reported an interesting finding that ABS decomposition can be simply tailored via adjusting the pore size of the material it deposited. We initiated this study from the preparation of mesoporous silica SBA-15 with uniform one-dimensional pore structure but different pore sizes, followed by ABS loading to investigate the effect. The results showed that ABS decomposition proceeded more easily on SBA-15 with larger pores, and the decomposition temperature declined as large as 40 °C with increasing pore size of SBA-15 from 4.8 to 11.8 nm. To further ascertain the real effect in NH3-SCR reaction, the Fe2O3/SBA-15 probe catalyst was prepared. It was found that the catalyst with larger mesopores exhibited much improved sulfur resistance, and quantitative analysis results obtained from Fourier transform infrared and ion chromatograph further proved that the deposited sulfates were greatly alleviated. The result of the present study demonstrates for the first time the vital role of pore size engineering in ABS decomposition and may open up new opportunities for designing NH3-SCR catalysts with excellent sulfur resistance.

17.
Materials (Basel) ; 12(3)2019 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-30696118

RESUMO

The dependencies of weight gain of 9-12 Cr ferritic-martensitic steels in supercritical water on each of seven principal independent variables (temperature, oxygen concentration, flow rate, exposure time, and key chemical composition and surface condition of steels) have been predicted using a supervised artificial neural network (ANN). The relative significance of each independent variable was uncovered by fuzzy curve analysis, which ranks temperature and exposure time as the most important. The optimized ANN, not only satisfactorily represents the experimentally-known non-linear relationships between the corrosion characteristics of F-M steels and the key independent variables (demonstrating the effectiveness of this technique), but also predicts and reveals that the effects of oxygen concentration on the weight gains, to a certain degree, is influenced by the flow rate and temperature. Finally, according to the ANN predicted-results, departure of oxidation kinetics from the parabolic law, and basic cause of chromium content in steel substrate influencing the corrosion rate, and the synergetic effects of dissolved oxygen concentration, flow rate, and temperature, are discussed and analyzed.

18.
Proc Natl Acad Sci U S A ; 116(3): 976-981, 2019 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-30591559

RESUMO

IFN-stimulated genes (ISGs) are essential effectors of the IFN-dependent antiviral immune response. Dysregulation of ISG expression can cause dysfunctional antiviral responses and autoimmune disorders. Epitranscriptomic regulation, such as N 6-methyladenosine (m6A) modification of mRNAs, plays key roles in diverse biological processes. Here, we found that the m6A "reader" YT521-B homology domain-containing family 3 (YTHDF3) suppresses ISG expression under basal conditions by promoting translation of the transcription corepressor forkhead box protein O3 (FOXO3). YTHDF3 cooperates with two cofactors, PABP1 and eIF4G2, to promote FOXO3 translation by binding to the translation initiation region of FOXO3 mRNA. Both the YTH and the P/Q/N-rich domains of YTHDF3 were required for FOXO3 RNA-binding capacity, however, METTL3-mediated m6A modification was not involved in the process observed. Moreover, YTHDF3-/- mice had increased ISG levels and were resistant to several viral infections. Our findings uncover the role of YTHDF3 as a negative regulator of antiviral immunity through the translational promotion of FOXO3 mRNA under homeostatic conditions, adding insight into the networks of RNA-binding protein-RNA interactions in homeostatically maintaining host antiviral immune function and preventing inflammatory response.


Assuntos
Proteína Forkhead Box O3 , Biossíntese de Proteínas , Proteínas de Ligação a RNA , Viroses/imunologia , Animais , Proteína Forkhead Box O3/genética , Proteína Forkhead Box O3/imunologia , Células HEK293 , Humanos , Interferons/genética , Interferons/imunologia , Metiltransferases/genética , Metiltransferases/imunologia , Camundongos , Camundongos Knockout , Biossíntese de Proteínas/genética , Biossíntese de Proteínas/imunologia , Células RAW 264.7 , RNA Mensageiro/genética , RNA Mensageiro/imunologia , Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/imunologia , Viroses/genética
19.
Anal Sci ; 34(12): 1385-1391, 2018 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-30175726

RESUMO

In this work, sodium dodecyl benzene sulfonate (SDBS) was used as a dispersing agent; a WO3 nanoparticle suspension was used as a sensing material. The SDBS-WO3 thin film/Sn-doped glass optical waveguide sensor element was prepared by spin coating. The sensing material was characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and field-emission scanning electron microscopy (FESEM). The gas-sensing characteristics of the fabricated sensors were studied at room temperature for various gases. The experimental results indicate that the sensor exhibited a high selective response toward SO2 and H2S and a low detection limit of 10 ppb to SO2 and H2S. The response/recovery times for SO2 and H2S were 2/23 and 2/18 s. However, during an electrochemical gas-sensing performance test of the SDBS-WO3 film at room temperature, the results indicated that the trend of the variation in resistance was consistent with the variation in the output light.

20.
J Thorac Dis ; 10(6): 3418-3427, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30069337

RESUMO

Background: Combined small cell lung cancer (C-SCLC) is defined as small cell lung cancer (SCLC) combined with any of non-small cell lung cancer (NSCLC) histological types, such as large cell carcinoma, squamous cell carcinoma, or adenocarcinoma. Since C-SCLC is an increasingly recognized subtype of small cell carcinoma, we conducted a retrospective study in our institution to explore the value of prophylactic cranial irradiation (PCI) in patients with C-SCLC treated by surgery. Methods: Between 2005 and 2014, the records of all consecutive patients with pathologically diagnosed C-SCLC after surgery in our institution were reviewed. Overall survival (OS), disease-free survival (DFS), and brain metastasis free survival (BMFS) were estimated by Kaplan-Meier method. Survival differences were evaluated by log-rank test, while multivariate analysis was performed by a Cox proportional hazards model. Results: Of the total 91 patients included in this analysis, 11 patients (12.1%) were in PCI group and 80 (87.9%) in non-PCI group. The 5-year cumulative incidence of brain metastasis in the whole group was 22.2% (26.3% in non-PCI group vs. 0% in PCI group), and 5-year OS rate was 44.1%. Patients treated with PCI had significantly longer OS (P=0.011) and DFS (P=0.013), also had the trend to live a longer BMFS with marginal significance (P=0.092) than non-PCI-treated patients. The multivariate analysis showed that PCI [hazard ratio (HR) =0.102, P=0.024] was one of independent prognostic factors of the OS in surgery-treated C-SCLC patients. Conclusions: C-SCLC patients have a relative high risk of developing brain metastases based on our study. These data showed that PCI could improve OS and DFS, as well as tend to decrease brain metastases in surgically resected C-SCLC. However, whether PCI could be part of comprehensive treatment modalities in C-SCLC should be assessed in prospective studies.

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