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1.
Food Chem ; 367: 130760, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34390911

RESUMO

Rapid volatile detection methods for seed vigour rely heavily on artificial ageing (AA), however the comparability of volatile organic compounds (VOCs) to natural ageing (NA) and practicability of the detection models were not well known. In this study, VOCs between AA and NA sweet corn seeds were compared and Partial Least Squares Regression (PLS-R) modelswere constructed based on AA to predict the seed vigour of NA. A total of 33 VOCs were identified, among which aldehydes showed the highest consistency between NA and AA. Furthermore, a AS-PLS-R model with variable importance in projection (VIP > 1) and Pearson Correlation Coefficient (r > 0.9) algorithms, which was built on 3 volatile markers: benzaldehyde monomer, n-nonanal, 1-butanol monomer, achieved the best performance (R2p of 0.901 and RMSEP of 0.050). Therefore, coupling Gas Chromatography- Ion Mobility Spectrometry (GC-IMS) with chemometrics can be an effective way to monitor and predict stored seeds vigour.


Assuntos
Compostos Orgânicos Voláteis , Cromatografia Gasosa-Espectrometria de Massas , Espectrometria de Mobilidade Iônica , Análise dos Mínimos Quadrados , Sementes/química , Compostos Orgânicos Voláteis/análise
2.
Anal Chem ; 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34643370

RESUMO

Single-cell-based genomics and transcriptomics analysis have revealed substantial cellular heterogeneity among seemingly identical cells. Knowledge of the cellular heterogeneity at multiomics levels is vital for a better understanding of tumor metastasis and drug resistance, stem cell differentiation, and embryonic development. However, unlike genomics and transcriptomics studies, single-cell characterization of metabolites, proteins, and post-translational modifications at the omics level remains challenging due to the lack of amplification methods and the wide diversity of these biomolecules. Therefore, new tools that are capable of investigating these unamplifiable "omes" from the same single cells are in high demand. In this work, a microwell chip was prepared and the internal surface was modified for hydrophilic interaction liquid chromatography-based tandem extraction of metabolites and proteins and subsequent protein digestion. Next, direct electrospray ionization mass spectrometry was adopted for single-cell metabolome identification, and a data-independent acquisition-mass spectrometry approach was established for simultaneous proteome profiling and phosphoproteome analysis without phosphopeptide enrichment. This integrated strategy resulted in 132 putatively annotated compounds, more than 1200 proteins, and the first large-scale phosphorylation data set from single-cell analysis. Application of this strategy in chemical perturbation studies provides a multiomics view of cellular changes, demonstrating its capability for more comprehensive investigation of cellular heterogeneity.

3.
Rev Sci Instrum ; 92(9): 095005, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34598513

RESUMO

Series elastic actuators (SEAs) have widely been adapted in robots where safe human-robot interaction is required for accurate and robust force control. Recent research on the SEAs has shown that the SEA with a user-defined variable stiffness possesses several advantages over the constant stiffness SEA, such as large force range and bandwidth while keeping low output impedance and high force fidelity. However, a limitation of this type of SEA is that an obvious hysteresis effect exists and the associated torque curves are nonlinear and vary with amplitudes. Conventional mathematical hysteresis models are usually developed with some kind of black-box modeling, and the model parameters are adjusted through parameter identification methods. It is challenging to tune the model parameters to match the experimental data well among inputs with different amplitudes, let alone the inverse model of the hysteresis, which is necessary to compensate the hysteresis effect in control. In this paper, a rotary SEA (rSEA) with nonlinear stiffness is proposed. A concept called "virtual deformation" is introduced to mathematically transform the nonlinear curve into a polyline hysteresis model. This eases torque estimation with respect to the deformation of the rSEA. A hysteresis compensation torque controller is implemented for precise torque control. A prototype of the rSEA was fabricated, and the experimental results verified modeling accuracy of the proposed model. Our results showed that, with the new model, the computation cost was greatly reduced while keeping the modeling accuracy almost the same compared with the nonlinear backlash model.

4.
Front Endocrinol (Lausanne) ; 12: 751213, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34603216

RESUMO

Objective: This study aimed to evaluate the feasibility and efficacy of ultrasound-guided percutaneous microwave ablation (MWA) in the treatment of low-risk papillary thyroid microcarcinoma (PTMC), and to observe the histopathological changes after MWA. Methods: MWA was performed under ultrasound guidance for 73 unifocal PTMC patients without clinically cervical or distant metastasis. The target ablation zone exceeded the tumor edge (judged by contrast-enhanced US) to avoid marginal residue and recurrence. Ultrasound evaluation was performed at 1 day, 1, 3, 6, 12 and 24 months after treatment, and thyroid function evaluation at the first 6 months. Repeated fine needle aspiration cytology or core needle biopsy pathology was performed at 3 or 6 months after MWA to evaluate residual tumors. Any adverse event associated with MWA was evaluated. Results: The follow-up after MWA lasted 6 (6, 12) months. Tumor volume decreased significantly from 0.06 mm3 (0.04, 0.11 mm3) to 0.03 mm3 (0.00, 0.06 mm3) at 12 months after MWA (P< 0.001), with a median volume reduction ratio of 80.28% (-7.43, 100%) and 16 cases (21.92%) presenting complete remission. The largest diameter, volume and ablation energy were found to be different in patients with and without complete remission 12 months after MWA. On histopathological examinations, no atypical or malignant follicular cells were identified after thermal ablation. The most common pathological characteristics were fibroblastic proliferation (34/39, 87.18%) and chronic inflammation (32/39, 82.05%), followed by infarction (21/39, 53.85%). Five patients were transferred to thyroidectomy and 4 of them were confirmed with local recurrence and/or lymph node metastasis. Serum thyrotropin decreased transiently after MWA (P< 0.01) but normalized thereafter. No serious and permanent complications were reported. Conclusions: MWA is a safe and effective treatment for low-risk PTMC. Fibroblastic proliferation and chronic inflammation are the most common pathological changes after MWA of PTMC.

5.
J Cell Mol Med ; 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34609072

RESUMO

Residue hepatocellular carcinoma (HCC) cells enduring hypoxic environment triggered by interventional embolization obtain more malignant potential with little clarified mechanism. The N6 -methyladenosine (m6 A) biological activity plays essential roles in diverse physiological processes. However, its role under hypoxic condition remains largely unexplored. RT-qPCR and Western blot were used to evaluate METTL14 expression in hypoxic HCC cells. MDA assay and electronic microscopy photography were used to evaluate ferroptosis. The correlation between SLC7A11 and METTL14 was conducted by bioinformatical analysis. Flow cytometry was used to verify the effect of SLC7A11 on ROS production. Cell counting kit-8 assay was performed to detect cells proliferation ability. Hypoxia triggered suppression of METTL14 in a HIF-1α-dependent manner potently abrogated ferroptosis of HCC cells. Mechanistic investigation identified SLC7A11 was a direct target of METTL14. Both in vitro and in vivo assay demonstrated that METTL14 induced m6 A modification at 5'UTR of SLC7A11 mRNA, which in turn underwent degradation relied on the YTHDF2-dependent pathway. Importantly, ectopic expression of SLC7A11 strongly blocked METTL14-induced tumour-suppressive effect in hypoxic HCC. Our investigations lay the emphasis on the hypoxia-regulated ferroptosis in HCC cells and identify the HIF-1α /METTL14/YTHDF2/SLC7A11 axis as a potential therapeutic target for the HCC interventional embolization treatment.

6.
Anal Chim Acta ; 1182: 338941, 2021 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-34602200

RESUMO

It is the first time to investigate local distribution patterns of mercury (Hg) in mice organs after Hg and Se exposure with detection of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Two batch of adult mice were employed to be exposed to inorganic mercury (iHg) and methylmercury (MeHg) with or without Se at the dose of 55 µmol kg-1. Tissue sections of brain, kidney, liver, and spleen from one batch mice were prepared to get local imaging of Hg by LA-ICP-MS. Tissues from another batch mice were used to quantify Hg and Se in tissues with ICP-MS after acid digestion. The results indicated that, for mice exposed to iHg, Hg mainly distributed in kidney, a little in liver, and hardly in brain and spleen; for mice exposed to MeHg, lower amount of Hg was found in kidney, liver and spleen, and almost no Hg was found in brain. It was interesting that for Hg and Se co-administration groups, higher level of Hg was observed in kidney, liver, spleen and even in brain than single Hg administration groups. In addition, Se level in organ tissues increased obviously not only in Se exposure group but also in MeHg exposure group, while the phenomenon was not observed in iHg exposure group. HepG2 cells were employed to investigate Se and Hg interactions in single cell level, similar bioaccumulation behavior of Hg was found between cells and mice organs. Higher level of Hg was observed in cells cultured with Se and Hg medium than cells cultured with single Hg medium. The results are expected to provide new insight to investigate Hg and Se interactions in animal bodies and in-vitro cells.


Assuntos
Terapia a Laser , Mercúrio , Compostos de Metilmercúrio , Selênio , Animais , Fígado , Mercúrio/toxicidade , Compostos de Metilmercúrio/toxicidade , Camundongos , Selênio/toxicidade
7.
Toxicol Lett ; 353: 1-12, 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34626813

RESUMO

Silicosis as the serious occupational disease is highly necessary to construct a suitable mouse model for disclosing mechanism of occurrence and development in this disease. Here, the volume-effect relationship and volume-based survival curves in mice who inhaled silica suspension intranasally were analyzed. Notable, the optimal volume 80 µl repeated-inhalation by nose to silica suspension in the inbred mouse C57BL/6 J with the highest susceptibility to silicosis led to a great entrance into the lung and a high survival rate after instillation. After repeated-exposure to 20 mg/mL, 80 µl silica for 16 days and then fed without silica exposure until 31 days, weight of mice showed a trend of first decrease and then recover. Moreover, the degree of pulmonary inflammation and fibrosis in mice were analyzed by pathological and immunohistochemistry staining. Transforming growth factor-beta (TGF-ß), smooth muscle alpha-actin (α-SMA) and collagen type-I (collagen I, Col-I) were significantly increased in the silica-exposed mouse lung at post-exposure day 16 compared with the controls. Sirius red stain and Micro-CT analysis showed that lung fibrosis formed at post-exposure day 31. This study highlights the critical importance of perfusion volume and repeated nasal drops in inducing inflammatory response and pulmonary fibrosis in silicosis.

8.
Mult Scler Relat Disord ; 56: 103314, 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34634624

RESUMO

OBJECTIVE: To determine the longitudinal changes in retinal microstructure, microvasculature, microcirculation, and axonal and neuronal functions in patients with relapsing-remitting multiple sclerosis (RRMS) over the time course of about two years. METHODS: A total of 30 patients (60 eyes) with RRMS were followed for a period of 27 ± 6 months and evaluated with a battery of clinical tests including low contrast letter acuity (LCLA), intraretinal layer thicknesses by optical coherence tomography (OCT), ganglion cell function by steady-state pattern electroretinography (PERG), axonal function by polarization-sensitive OCT, volumetric vessel density (VVD) by OCT angiography, and retinal tissue perfusion (RTP) by retinal function imager. RESULTS: Axonal function measured as retinal nerve fiber layer birefringence in the temporal quadrant and vessel density in the deep vascular plexus were significantly decreased at 2-year follow-up (P < 0.05). Subgroup analyses showed that the increased retinal blood flow volume occurred in patients with no evidence of disease activity (NEDA), and with stable or improved visual function (P < 0.05). There was no significant difference in the expanded disability state scale, LCLA, RTP, VVD, or PERG measures between the two visits (P > 0.05). CONCLUSION: To our best knowledge, this is the first 2-year prospective comprehensive study with a detailed assessment of retinal microstructure and neuronal functions in patients with RRMS. The recovery of retinal microcirculation occurred in patients with NEDA, and stable or improved visual function, suggesting these measurements as potential imaging biomarkers for monitoring disease progression.

9.
Anal Chim Acta ; 1183: 338973, 2021 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-34627508

RESUMO

In this study, the application of carbon dots (CDs) modified NaYF4:Yb, Er nanoparticles (UCNPs@CDs) as the fluorescent nanoprobe for simultaneous detection of Fe2+ and Fe3+ was investigated. Fe3+ quantification (5-80 µmol L-1) was achieved, as a result of Fe3+ induced fluorescence quenching of UCNPs@CDs at 434 nm (under the 336 nm excitation). The chelate (Fe2+-phen) formed by Fe2+ and 1,10-phenanthroline had a broad absorption centered at 510 nm, due to inner filter effect (IFE), Fe2+ quantification (4-120 µmol L-1) was achieved as a result of (Fe2+-phen) induced fluorescence quenching of UCNPs@CDs at 545 nm (under the 980 nm excitation). The resultant UCNPs@CDs probe, with excellent anti-interference capability, favorable fluorescence stability, and convincing performance in real sample analysis, showed promising application in simultaneous detection of Fe2+ and Fe3+.


Assuntos
Carbono , Nanopartículas , Íons , Ferro , Espectrometria de Fluorescência
10.
Chemosphere ; : 132540, 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34648792

RESUMO

No large-scale investigations on fate of land-based antibiotic resistance genes (ARGs) in marginal sea have been reported. The Yellow Sea which is an important marginal sea was selected to investigate the fate, territorial differentiation, and drivers of target ARGs in marginal-sea sediments. ARGs might spread from land to sea with the average absolute abundance of total ARGs in marine/coastal sediments reaching 1.23 × 104/9.79 × 104 copies/g. The Yellow Sea Cold Water Mass was firstly observed to possibly have potential inhibition effect on occurrence of ARGs in marine sediments. Marine sediments showed significant difference from coastal sediments by considering ARGs, microbial community, and sediment features. Network analysis showed that interaction between ARGs and microbial community in coastal sediments was more frequent than that in marine sediment. The anthropogenic factors posed high positive effect on ARGs in marine/coastal sediments with contribution coefficient of 0.524/1.094 while bacterial community mainly posed positive effect on ARGs in marine sediments with contribution coefficient of 0.475, illustrating that spread and proliferation of land-based ARGs in marine sediments might be mainly affected by anthropogenic and microbial factors. These findings provided new information on fate and drivers of ARGs in marginal sea.

11.
Spectrochim Acta A Mol Biomol Spectrosc ; 266: 120409, 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34607091

RESUMO

Biothiols play an important role in many physiological and pathological processes, especially in the occurrence of oxidative stress caused by abnormal cysteine (Cys) concentration. Therefore, it is particularly critical to develop a method that can specifically identify Cys to avoid interference from other biological analytes. However, most Cys-specific fluorescent probes are difficult to distinguish between homocysteine (Hcy) and glutathione (GSH). In this work, to avoid the interference of Hcy and GSH, we developed a fluorescent probe triarylimidazole-naphthalimide-piperazine-sulfonyl benzoxadiazole (TNP-SBD-Cl) based on fluorescence resonance energy transfer (FRET) on platform of naphthalimide-sulfonyl benzoxadiazole (SBD), the main SBD 4-chlorine groups have mild reactivity to undergo substitution and rearrangement to distinguish Hcy and GSH. The TNP-SBD-Cl response to Cys would turn on FRET and generate a new yellow fluorescence with a large Stokes shift (157 nm), and with excellent selectivity and low detection limit (0.87 µM). Moreover, TNP-SBD-Cl can be used to monitor Cys in living HeLa cells with low cytotoxicity, suggesting that it has markedly diagnostic significance in physiological and pathological processes.

12.
ACS Appl Mater Interfaces ; 13(36): 43668-43675, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34473482

RESUMO

The efficient recognition of circulating tumor cells (CTCs) with an aptamer probe confers numerous benefits; however, the stability and binding affinity of aptamers are significantly hampered in real biological sample matrices. Inspired by the efficient preying mechanism by multiplex tubing feet and endoskeletons of sea urchins, we engineered a superefficient biomimetic single-CTC recognition platform by conjugating dual-multivalent-aptamers (DMAs) Sgc8 and SYL3C onto AuNPs to form a sea urchin-like nanoprobe (sea urchin-DMA-AuNPs). Aptamers Sgc8 and SYL3C selectively bind with the biomarker proteins PTK7 and EpCAM expressed on the surface of CTCs. CTCs were captured with 100% efficiency, followed by sorting on a specially designed multifunctional microfluidic configuration, integrating a single-CTC separation unit and a hydrodynamic filtrating purification unit. After sorting, background-free analysis of biomarker proteins in single CTCs was undertaken with inductively coupled plasma mass spectrometry by measuring the amount of 197Au isotope in sea urchin-DMA-AuNPs. With respect to a single-aptamer nanoprobe/-interface, the dual-aptamer nanoprobe improves the binding efficiency by more than 200% (Kd < 0.35 nM). The microchip facilitates the recognition of single CTCs with a sorting separation rate of 93.6% at a flow rate of 60 µL min-1, and it exhibits 73.8 ± 5.0% measurement efficiency for single CTCs. The present strategy ensures the manipulation and detection of a single CTC in 100 µL of whole blood within 1 h.

13.
Sci Total Environ ; 804: 150140, 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34509841

RESUMO

With the rapid development of society, the soil and water environments in many countries are suffering from severe pollution. Pollutants in different phases will eventually gather into the soil and water environments, and a series of migrations and transformations will take place at ecohydrological interfaces with water flow. However, it is still not clear how ecohydrological interfaces affect the migration and the transformation of pollutants. Therefore, this paper summarizes the physical, ecological, and biogeochemical characteristics of ecohydrological interfaces on the basis of introducing the development history of ecohydrology and the concept of ecohydrological interfaces. The effects of ecohydrological interfaces on the migration and transformation of heavy metals, organic pollutants, and carbon­nitrogen­phosphorus (C-N-P) pollutants are emphasized. Lastly, the prospects of applying ecohydrological interfaces for the removal of pollutants from the soil and water environment are put forward, including strengthening the ability to monitor and simulate ecohydrological systems at micro and macro scales, enhancing interdisciplinary research, and identifying main influencing factors that can provide theoretical basis and technical support.

14.
Nucleic Acids Res ; 2021 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-34570217

RESUMO

Interpreting the molecular mechanism of genomic variations and their causal relationship with diseases/traits are important and challenging problems in the human genetic study. To provide comprehensive and context-specific variant annotations for biologists and clinicians, here, by systematically integrating over 4TB genomic/epigenomic profiles and frequently-used annotation databases from various biological domains, we develop a variant annotation database, called VannoPortal. In general, the database has following major features: (i) systematically integrates 40 genome-wide variant annotations and prediction scores regarding allele frequency, linkage disequilibrium, evolutionary signature, disease/trait association, tissue/cell type-specific epigenome, base-wise functional prediction, allelic imbalance and pathogenicity; (ii) equips with our recent novel index system and parallel random-sweep searching algorithms for efficient management of backend databases and information extraction; (iii) greatly expands context-dependent variant annotation to incorporate large-scale epigenomic maps and regulatory profiles (such as EpiMap) across over 33 tissue/cell types; (iv) compiles many genome-scale base-wise prediction scores for regulatory/pathogenic variant classification beyond protein-coding region; (v) enables fast retrieval and direct comparison of functional evidence among linked variants using highly interactive web panel in addition to plain table; (vi) introduces many visualization functions for more efficient identification and interpretation of functional variants in single web page. VannoPortal is freely available at http://mulinlab.org/vportal.

15.
J Comput Assist Tomogr ; 45(5): 788-794, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34546683

RESUMO

OBJECTIVE: The aim of this study was to analyze the computed tomography (CT), fluorine-18 fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT), and clinical findings of pulmonary epithelioid hemangioendothelioma. METHODS: Eighteen patients were included. We reviewed CT and PET/CT features and clinical information. Spearman correlation analysis was used to reveal the correlation between lesion size and maximum standardized uptake values (SUVmax). RESULTS: Eleven patients complained of different symptoms. The serum levels of tumor markers were elevated in 8 patients (44.4%). Multiple lesions were shown in 14 cases (77.8%). Eleven cases (61.1%) presented as bilateral multiple perivascular lesions. The size of the largest nodule was less than 20 mm in diameter in 11 cases (61.1%). Eleven cases underwent PET/CT examination, and 8 cases (72.7%) had increased FDG uptake. There was a positive correlation (Spearman correlation coefficient, 0.56; P = 0.009) between lesion size and SUVmax. Extrapulmonary involvement was shown in 7 cases, including the liver and bone. CONCLUSIONS: Pulmonary epithelioid hemangioendothelioma on CT scan usually showed multiple bilateral perivascular lung nodules, generally measuring less than 20 mm in diameter. Lesions usually showed increased FDG uptake on PET/CT scans. There was a positive correlation between lesion size and SUVmax. The liver and bone were the most frequently involved extrapulmonary organs.


Assuntos
Fluordesoxiglucose F18 , Hemangioendotelioma Epitelioide/diagnóstico por imagem , Neoplasias Pulmonares/diagnóstico por imagem , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada/métodos , Tomografia Computadorizada por Raios X/métodos , Adolescente , Adulto , Idoso , Feminino , Humanos , Pulmão/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto Jovem
16.
Colloids Surf B Biointerfaces ; 208: 112116, 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34564039

RESUMO

Osteoarthritis disease can easily lead to articular cartilage degeneration and subchondral bone damage, so the demand for suitable articular substitutes is gradually increasing. In order to simulate the complex environment of different layers in natural joint, we fabricate the continuous one-phase gradient scaffold. In the study, CS (chitosan) was modified with SH (sodium hyaluronate) and GO (graphene oxide) to form the whole scaffold. nHAP (Nano-hydroxyapatite) was in situ generated with gradient distribution in the scaffold. Continuous interface can better imitate the combination style of cartilage and subchondral bone at joint. The diverseness of scaffold's different layer in water absorption/retention rate and mechanical property is similar to the difference of articular cartilage and subchondral bone. Meanwhile, the cell experiments demonstrated that the bionic scaffold can well promote the proliferation of bone marrow mesenchymal stem cell. Articular defect model further confirmed that the scaffold can better induce articular regeneration. Herein, the prepared scaffold might be an excellent candidate for endogenous articular repair.

17.
Appl Microbiol Biotechnol ; 105(19): 7265-7282, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34491399

RESUMO

In the present study, we aimed to investigate the antibacterial activity and mechanisms of plectasin-derived peptide NZ2114 in vitro and its therapeutic effects in vivo on broilers challenged with Clostridium perfringens. In vitro assay showed that NZ2114 had potent (minimal inhibitory concentration, 0.91 µM) and rapid antibacterial activity (99.9% reduction within 2 h), and the dual antibacterial mechanisms (including interfering with the cell membrane and intracellular DNA) against C. perfringens CVCC 2030. In vivo study, NZ2114 tended to increase linearly and quadratically the average daily gain as NZ2114 level increased and was the highest at 20 mg/L. NZ2114 at 10 ~ 40 mg/L dramatically reduced jejunal lesion score. Besides, the levels of IL-6, TNF-α, and IL-1ß tended to downregulate linearly and quadratically as the NZ2114 level increased and were all the lowest at the dose of 20 mg/L. NZ2114 significantly upregulated those levels of IgA, IgG, IgM, and sIgA with a linear and quadratic dose effect, with the highest IgA, IgG, IgM, and sIgA at 20 mg/L. Finally, NZ2114 tended to linearly and quadratically increase the numerical value of crypt depth, with the lowest value at 40 mg/L. Lincomycin only dramatically reduced the jejunal lesion score and increased the numerical value of crypt depth. These results indicate that NZ2114 has the potential as a new alternative to antibiotics for the treatment of C. perfringens-induced necrotic enteritis infection.Key points• NZ2114 could kill C. perfringens by dual antibacterial mechanisms• Broiler necrotic enteritis model induced by C. perfringens was established• NZ2114 treatment could ameliorate C. perfringens-induced necrotic enteritis.


Assuntos
Infecções por Clostridium/veterinária , Clostridium perfringens , Defensinas/farmacologia , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Animais , Galinhas , Infecções por Clostridium/tratamento farmacológico , Clostridium perfringens/efeitos dos fármacos , Aves Domésticas
18.
Mol Med Rep ; 24(5)2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34490484

RESUMO

Naringin (Nar) is one of the natural glycosides extracted from pomelo and other citrus fruits. It has various pharmacological activities, including anti­inflammatory, antioxidant, anti­proliferative and anti­cancer. However, the underlying mechanisms by which Nar regulates apoptosis and autophagy in gastric cancer remain unclear. Thus, the present study aimed to assess the therapeutic effect of Nar and the underlying mechanisms. SNU­1 cell proliferation was determined using Cell Counting Kit­8 assay. Cell morphological changes were observed under a phase­contrast microscope. The changes in the cell cycle were determined using flow cytometry analysis and the changes in cell apoptosis were determined using flow cytometry, Hoechst 33258 and TUNEL staining. The protein levels pertaining to the PI3K/AKT pathway and cell apoptosis and autophagy were monitored using western blot analysis. The results demonstrated that Nar significantly inhibited SNU­1 cell growth and induced cell cycle arrest in the G0/G1 phase and cell apoptosis. Further mechanistic studies demonstrated that Nar blocked the PI3K/AKT pathway, activated cell autophagy and stimulated the expression of apoptosis­associated protein cleaved caspase 3 and Bax, but decreased the expression of Bcl­2. Preincubating SNU­1 cells with 3­methyladenine, a cell­autophagy inhibitor, significantly alleviated the effects of Nar in promoting cell apoptosis and cleaved caspase 3 expression. It was concluded that Nar promoted SNU­1 cell apoptosis via blocking the PI3K/AKT signaling pathway and activating cell autophagy.

20.
Sci Rep ; 11(1): 19345, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34588562

RESUMO

Abiotic stresses, including cold and drought, negatively affect maize (Zea mays L.) seed field emergence and later yield and quality. In order to reveal the molecular mechanism of maize seed resistance to abiotic stress at seed germination, the global transcriptome of high- vigour variety Zhongdi175 exposed to cold- and drought- stress was analyzed by RNA-seq. In the comparison between the control and different stressed sample, 12,299 differentially expressed genes (DEGs) were detected, of which 9605 and 7837 DEGs were identified under cold- and drought- stress, respectively. Functional annotation analysis suggested that stress response mediated by the pathways involving ribosome, phenylpropanoid biosynthesis and biosynthesis of secondary metabolites, among others. Of the obtained DEGs (12,299), 5,143 genes are common to cold- and drought- stress, at least 2248 TFs in 56 TF families were identified that are involved in cold and/or drought treatments during seed germination, including bHLH, NAC, MYB and WRKY families, which suggested that common mechanisms may be originated during maize seed germination in response to different abiotic stresses. This study will provide a better understanding of the molecular mechanism of response to abiotic stress during maize seed germination, and could be useful for cultivar improvement and breeding of high vigour maize cultivars.

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