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1.
Talanta ; 206: 120204, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31514833

RESUMO

As a rapid and facile means for foodborne bacteria detection in situ, lateral flow immunoassay (LFA) still has intrinsic limitations in the construction of the existing sandwich LFA format, e.g. screening difficulties of paired antibodies (Abs), poor stability of Ab probe, etc. Here, combined the strong affinity of antibiotic with the superior specificity of antibody molecules, a novel and robust LFA based on a dual recognition strategy and magnetic separation was designed to achieve specific and sensitive determination of Salmonella enteritidis (S. enteritidis). In this work, ampicillin (Amp), a broad-spectrum antibiotic against bacteria, was employed as an ideal Ab replacer to anchor cells of target bacteria. By coating Amp on magnetite nanoparticles (MNPs), the Amp-MNPs showed remarkable binding, separation and enrichment capacities toward bacteria even under complex sample matrices. To ensure the selectivity of this protocol, anti-S. enteritidis monoclonal antibody was then adopted as the second anchoring agent to form a sandwich complex with Amp-MNPs. Based on these facts, S. enteritidis, as low as 102-103 CFU/mL, could be detected by naked eyes in food samples. Therefore, this creative antibiotic-bacteria-antibody LFA sandwich pattern shows great application potential in the monitoring of food contamination and infectious diseases caused by pathogenic bacteria. Compared to the common paired Abs based sandwich method, the proposed approach was cost-effective, non-labor intensive, stable, sensitive and efficient.

2.
J Hazard Mater ; 382: 121058, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31450213

RESUMO

In present work, the degradation of antibiotic and inactivation of antibiotic resistance genes (ARGs) in cephalosporin C fermentation (CEPF) residues were performed using ionizing radiation, ozonation and thermal treatment. The results showed that the three treatment methods could degrade cephalosporin C effectively, with the removal efficiency of 85.5% for radiation at dose of 100 kGy, 79.9% for ozonation at dosage of 5.2 g O3/L, and 71.9% and 87.3% for thermal treatment at 60 °C and 90 °C for 4 h. The cephalosporin resistance gene tolC was detected in the raw CEPF residues, and its abundance was decrease 74.2% by radiation, 64.6% by ozonation and 26.9%-37.1% by thermal treatment respectively. The presence of protein, glucose and acetate in the CEPF residues had inhibitive influence on the degradation of cephalosporin C by ionizing radiation, and the effect was more significant when the antibiotic concentration was lower. The total content of COD, polysaccharides and protein changed slightly after radiation and thermal treatment, while they were decreased greatly by ozonation. The primary techno-economic analysis showed that the operational cost of ionizing radiation by electron beam at 50 kGy ($5.2/m3) was comparable to thermal treatment ($4.3-7.9/m3), which was more economical than ozonation ($14.6/m3).

3.
J Hazard Mater ; 383: 121126, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31505428

RESUMO

Here, TPB (triphenylbenzene) - DMTP (dimethoxyterephthaldehyde) -COF was prepared, characterized and used as effective adsorbent for the removal of sulfamerazine (SMT) from aqueous solution. Its adsorption characteristics and mechanism were explored. With large channel (∼3.3 nm), high specific surface area (2115 m2/g), and high crystallite, TPB-DMTP-COF showed high adsorption capacity (209 mg/g), fast adsorption equilibrium (80 min), and good reusability. Natural pH condition was optimal for its adsorption capacity, while electrostatic repulsion between TPB-DMTP-COF and SMT accounted for the low adsorption performance at acidic or alkaline conditions. According to the DFT method, SMT molecules adsorbed in the pore-sites of COFs via C-H···π interaction was the predominant and stable adsorption configuration accounting for the efficient removal of SMT in large quantity. This study revealed the great adsorption potential of COFs skeleton itself in the application of environmental remediation.

4.
Chemosphere ; 239: 124812, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31521932

RESUMO

Carbon-based materials have been studied as metal-free catalyst for persulfate activation. At present, the activation performance of carbon materials for persulfate is usually characterized by the removal efficiency of organic pollutants. However, the kinetics of persulfate activation by carbon materials has not been investigated. In this study, the kinetics of peroxymonosulfate (PMS) activation by reduced graphene oxide (RGO) and sludge-derived biochar (BC) were investigated. The experimental results showed that the kinetics of PMS activation followed the two-phase kinetic model (fast phase (a1) and slow phase (a2)). In the absence of organic pollutants, the a1 and a2 were calculated to be 0.320 and 0.0471 min-1 for BC, respectively, and 0.322 and 0.0850 min-1 for RGO, respectively. Based on the characterization of BC and RGO, it can be concluded that the fast phase was mainly due to the formation of surface-bound active species. Competitive adsorption between PMS and targeted pollutants decreased the kinetic constant for the first phase (a1) and the kinetic constant for the second phase (a2) for both BC and RGO. The value of a1 and a2 increased for BC after the addition of phenol, due to the enhanced PMS activation by surface adsorbed phenol. The increase of phenol concentration decreased slightly the value of a1 and a2. The increase of PMS concentration increased significantly the value of a1 and a2. The decrease of a1 and a2 in repeated use of carbon materials could be due to the decrease of oxygen-containing functional groups and defect intensity.

5.
J Hazard Mater ; : 121669, 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31740303

RESUMO

In this study, nitrogen, sulfur and oxygen co-doped carbon armored cobalt sulfide (Co/Co9S8@N-S-O-C) composite was synthesized, characterized and used to activate peroxymonosulfate (PMS) for the degradation of sulfamethoxazole (SMX). SMX (0.04 mM) can be completely degraded within 20 min in the presence of 0.8 mM PMS and 0.1 g/L Co/Co9S8@N-S-O-C composite. The first-order kinetics constant of SMX degradation was 0.307 min-1, and the mineralization of SMX was 30.1 %. The Quenching experiments of the free radicals and the identification of degradation products demonstrated that sulfate radicals played a dominant role in SMX degradation. The degradation rate of SMX increased with temperature, and activation energy was calculated to be 48.6 kJ/mol. The degradation rate of SMX increased firstly then decreased with increase of pH. Chloridion and humic acid decreased the degradation rate of SMX no matter what their initial concentration was. The effect of carbonate on SMX degradation depended on its initial concentration. Co/Co9S8@N-S-O-C composite showed good stability, the removal efficiency of SMX was 98.4 % in the fifth experiment. Based on the characterization results of the catalyst before and after use, it was concluded that cobalt, sulfur, pyridnic N and graphitic N were responsible for PMS activation.

6.
Sci Total Environ ; 701: 135023, 2019 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-31715480

RESUMO

Antibiotics are becoming emerging contaminants due to their extensive production and consumption, which have caused hazards to the ecological environment and human health. Various techniques have been studied to remove antibiotics from water and wastewater, including biological, physical and chemical methods. Among them, advanced oxidation processes (AOPs) have received increasing attention due to their fast reaction rate and strong oxidation capability, which are effective for the degradation of antibiotics in aquatic environments. In this review paper, a variety of AOPs, such as Fenton or Fenton-like reaction, ozonation or catalytic ozonation, photocatalytic oxidation, electrochemical oxidation, and ionizing radiation were briefly introduced, including their principles, characteristics, main influencing factors and applications. The current applications of AOPs for the degradation of antibiotics in water and wastewater were analyzed and summarized, the concluding remarks were given and their future perspectives and challenges were discussed.

7.
J Hazard Mater ; : 121596, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31732342

RESUMO

Covalent organic frameworks (COFs) are emerging and promising adsorbents for the adsorptive removal of many types of pollutants. However, most COFs fabricated by various methods are in the form of microcrystalline powders, making them difficult in separation. In this study, magnetic COFs were prepared by a simple impregnation method. The as-prepared samples with Ms = 5.2 emu g-1 showed a good magnetic separation capability. Additionally, the adsorption performance of magnetic COFs towards antibiotics (e.g., diclofenac and sulfamethazine) was also studied. Various adsorption kinetic models (e.g., the pseudo first-order, the pseudo second-order, and mixed-order models) and adsorption isotherm models (e.g., the Freundlich, the Langmuir, and the Sips models) were used to study their adsorption kinetics and isotherms. Furthermore, density functional theory (DFT) was utilized to predict the interaction between adsorbents and adsorbates and to explain the partial adsorption mechanisms.

8.
Mar Pollut Bull ; 145: 547-554, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31590822

RESUMO

Microplastics are becoming a global concern due to their potential to accumulate pollutants in aquatic environments. In this paper, sulfamethazine (SMT) sorption onto six types of microplastics, including polyamide (PA), polyethylene (PE), polyethylene terephthalate (PET), polypropylene (PP), polystyrene (PS), and polyvinyl chloride (PVC) was investigated by experimental and molecular dynamics simulation methods. The experimental results indicated that SMX sorption reached equilibrium within 16 h. The kinetics of SMT sorption by PA, PVC, PE, and PP could be fitted by pseudo first-order model, while SMT sorption by PA and PET could be described by pseudo second-order model. The partition coefficient Kd values were 38.7, 23.5, 21.0, 22.6, 18.6 and 15.1 L·kg-1 for PA, PE, PS, PET, PVC and PP, respectively. SMT sorption onto microplastics decreased when pH and salinity increased. The molecular dynamics simulation results indicated that the main mechanisms involved in sorption are electrostatic and Van der Waals interaction.

9.
J Hazard Mater ; 384: 121335, 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31590081

RESUMO

Antibiotic fermentation residues is a hazardous waste due to the existence of residual antibiotics and antibiotic resistance genes (ARGs), probably leading to the induction and spread of antibiotic resistant bacteria (ARB) in the environment, which could pose potential harm to the ecosystem and human health. It is urgent to develop an effective technology to remove the residual antibiotics and ARGs. In this study, the anaerobic digestion combined with gamma irradiation was applied for the disposal and utilization of cephalosporin C fermentation residues. The experimental results showed that the antibacterial activities of cephalosporin C against Staphylococcus aureus were significantly decreased after anaerobic digestion. The removal of tolC, a multidrug resistant gene, was improved up to 100% by the combination of gamma irradiation and anaerobic digestion compared to solely anaerobic digestion process, which may be due to the changes of microbial community structures induced by gamma irradiation.

10.
Carbohydr Polym ; 226: 115283, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31582088

RESUMO

Deproteinization is a crucial step for the purification of polysaccharides from natural biomass. However, traditional deproteinization technologies often suffer from complicated operating processes and the usage of toxic chemical reagents. Herein, an advanced and universal deproteinization method based on dual-functional adsorbent consisted of preferential protein adsorption coating of tannic acid-FeIII (TA-FeIII) and magnetic Fe3O4 (Fe3O4@TA-FeIII, abbreviated as FTF NPs) was developed. The proposed FTF NPs showed remarkable efficiency to remove protein from Lycium barbarum L. polysaccharides (LBPs) with deproteinization ratio of 96%, higher than the typical Sevag method (85%). Detailed adsorption kinetics studies demonstrated deproteinization process reached equilibrium after 10 min, faster than other reported deproteinization adsorbents. Furthermore, FTF NPs are structurally and functionally nondestructive as regards LBPs without using organic reagents. Also, it exhibited widespread-use deproteinization performance to several common plant species. Therefore, the proposed nano-separation based on TA-FeIII complex is an advanced and universal tool to high-efficiently deproteinize plant polysaccharides.

11.
ACS Sens ; 4(11): 2980-2987, 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31645102

RESUMO

In this work, we introduce our recent finding that the carbon fiber paper (CFP) treated by simple air annealing (OCFP) could be used for exceptionally high-performance electrochemical nitrite sensing. The air-annealing process endows the pristine CFP with higher defective edge/plane sites, more oxygen-containing functional groups, higher roughness, and improved wettability. The electrochemical studies show that the OCFP exhibits excellent sensing performance for nitrite, with an ultralow determination limit of 0.1 µM and a detection limit of 0.07 µM, an ultrawide linear determination range of 0.1-3838.5 µM, a fast current response of 1 s, and a high sensitivity of 930.4 µA mM-1 cm-2. These performance values are comparable or even superior to those for most reported noble- or transition-metal-based advanced nitrite sensors. Besides, this electrode also presents satisfactory stability, reproducibility, and feasibility of nitrite sensing in food samples. As an ideal monolithic and metal-free catalyst with ultrahigh and stable detection performance, the OCFP has a high potential to be integrated into next-generation electrochemical sensing devices.

12.
J Orthop Res ; 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31608495

RESUMO

Lumbar facet joint osteoarthritis (LFJ OA) is regarded as one of the common causes of low back pain (LBP). The pathogenesis and underlying mechanism of this disease are largely unknown, there is still no effective disease-modifying therapy. This study aims to investigate the efficacy of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) on the pathogenesis and behavioral signs of LBP in the LFJ OA mouse model. The pathogenetic change in cartilage and aberrant nerve invasion in the subchondral bone of LFJ in a mouse model after treatment with BMSC-exosomes was evaluated. BMSC-exosomes could relieve pain via abrogation of aberrant CGRP-positive nerve and abnormal H-type vessel formation in the subchondral bone of LFJ. Moreover, BMSC-exosomes attenuated cartilage degeneration and inhibited tartrate-resistant acid phosphatase expression and RANKL-RANK-TRAF6 signaling activation to facilitate subchondral bone remodeling. These results indicated that BMSC-exosomes could relive behavioral signs of LBP and pathological processes in LFJ OA. BMSC-exosomes have a prominent protective effect and might be a potential therapeutic option for the treatment of LFJ OA causing LBP. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.

13.
Chemosphere ; 241: 125002, 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31590027

RESUMO

Iron and copper bimetallic MOF material (FexCu1-x(BDC)) as a novel Fenton-like catalyst was prepared by a simple solvothermal method, and its performances were evaluated in the catalytic degradation of sulfamethoxazole (SMX) in the presence of hydrogen peroxide. The results indicated that the FexCu1-x(BDC)/H2O2 system was highly effective for SMX degradation over a wide pH range (4.0-8.6). At initial solution pH of 5.6, the bimetallic Fe0.75Cu0.25(BDC) catalyst exhibited a 100% SMX (20 mg L-1) removal within 120 min, which was superior to the SMX removal efficiency over monometallic Fe(BDC) and Cu(BDC) catalysts. Combined with the physical-chemical characterization, the synergistic effect between Fe and Cu species were responsible for the efficient catalytic activity. Moreover, the Fe0.75Cu0.25(BDC) catalyst showed good reusability for SMX degradation. The possible reaction mechanism in FexCu1-x(BDC)/H2O2 system was also tentatively proposed. This work has not only suggested the potential of bimetallic FexCu1-x(BDC) catalysts in Fenton-like treatment of antibiotics, but also provided useful information to develop MOF-based catalysts for efficient environmental remediation.

14.
Inorg Chem ; 58(19): 12573-12581, 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31509392

RESUMO

The worsening pollution due to mercury species makes it inevitable to explore prospective versatile materials, which not only can detect mercury ions (Hg2+) with high sensitivity but also possesses efficient capture and removal ability. In this study, a series of classic organic ligand-based luminescence MOFs materials with high oxidation state central metals (Al3+, Zr4+, Cr3+, Fe3+, and Ti4+) were synthesized and were screened to achieve simultaneously Hg2+ detection and removal through the strong coordination of amino groups or nitrogen centers with Hg2+ and the intrinsic fluorescence intensity of MOFs regulated by the ligand-to-metal charge transfer (LMCT) effect. Among these checked materials, NH2-MIL-53(Al) exhibited the excellent ability for Hg2+ detection with wide response interval (1-17.3 µM), low detection limit (0.15 µM), good selectivity, wide pH adaptation (4.0-10.0), and strong anti-interference ability. Meanwhile, the resultant NH2-MIL-53(Al) possessed an efficient removal capability toward Hg2+, accompanied by a fast uptake kinetics (within 60 min) and large loading capacity (153.85 mg g-1). Furthermore, NH2-MIL-53(Al) also displayed satisfactory stability before and after Hg2+ treatment because of the formation of strong coordination bonds between high oxidation state aluminum (Al3+) and organic carboxylate ligands. Notably, the prepared NH2-MIL-53(Al) had no significant loss of adsorption performance even after being reused four times. All of these superior properties render the smart NH2-MIL-53(Al) nanohexahedron a great potential for simultaneous Hg2+ detection and removal from water.

15.
Genes (Basel) ; 10(9)2019 09 17.
Artigo em Inglês | MEDLINE | ID: mdl-31533315

RESUMO

Rice, being a major staple food crop and sensitive to salinity conditions, bears heavy yield losses due to saline soil. Although some salt responsive genes have been identified in rice, their applications in developing salt tolerant cultivars have resulted in limited achievements. Herein, we used bioinformatic approaches to perform a meta-analysis of three transcriptome datasets from salinity and control conditions in order to reveal novel genes and the molecular pathways underlying rice response to salt. From a total of 28,432 expressed genes, we identify 457 core differentially expressed genes (DEGs) constitutively responding to salt, regardless of the stress duration, genotype, or the tissue. Gene co-expression analysis divided the core DEGs into three different modules, each of them contributing to salt response in a unique metabolic pathway. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses highlighted key biological processes and metabolic pathways involved in the salt response. We identified important novel hub genes encoding proteins of different families including CAM, DUF630/632, DUF581, CHL27, PP2-13, LEA4-5, and transcription factors, which could be functionally characterized using reverse genetic experiments. This novel repertoire of candidate genes related to salt response in rice will be useful for engineering salt tolerant varieties.

16.
Chemosphere ; 235: 985-994, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31561315

RESUMO

In this paper, the growth of S. bicolor in Cd-polluted sandy clay loam soil in north China, Cd accumulation in plant and the corresponding soil microbial community were characterized when the plant matured (140 d of growth). Cadmium promoted the growth of mature S. bicolor with higher height and heavier dry mass, especially at the spiked level of 1 mg kg-1 soil (P < 0.05). The higher microbial diversity was found under Cd stress at the spiked level of 15 mg kg-1, which basically corresponded with its influence on the plant growth. High-throughput sequencing data demonstrated that the predominant bacterial phyla include Proteobacteria (35.99% for Cd-polluted soil and 35.22% for the control soil), Chloroflexi (21.33% and 20.58%), Actinobacteria (12.00% and 12.89%), Acidobacteria (7.47% and 11.14%), Bacteroidetes (7.37% and 6.96%), Gemmatimonadetes (5.60% and 6.65%), Firmicutes (2.82% and 1.86%), Planctomycetes (2.47% and 0.95%), Saccharibacteria (1.26% and 1.11%). The predominant fungal phyla was Ascomycota, with the relative abundance of 89.96% for the control soil and 86.2% for the Cd-polluted soil. S. bicolor could grow well in sandy clay loam soil in northern China at low Cd lvel, but it could not accumulate cadmium at higher cadmium level. S. bicolor could be used for phytoextraction of cadmium from the lightly Cd-polluted soil.


Assuntos
Bactérias/metabolismo , Biodegradação Ambiental , Cádmio/metabolismo , Microbiologia do Solo , Poluentes do Solo/metabolismo , Solo/química , Sorghum/metabolismo , Cádmio/análise , China , Microbiota , Desenvolvimento Vegetal/efeitos dos fármacos , Poluentes do Solo/análise , Sorghum/crescimento & desenvolvimento
17.
Inorg Chem ; 58(17): 11382-11388, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31402664

RESUMO

Enzyme-like metal-organic frameworks (MOFs) are currently one type of starring material in the fields of artificial enzymes and analytical sensing. However, there has been little progress in making use of the MOF structures based on the catalytically active metal center with multiple valences. Herein, we report a mixed-valence Ce-MOF (Ce-BPyDC) that can exhibit both oxidase-like and peroxidase-like activities. Ce-BPyDC was synthesized by a facile hydrothermal method, which preserves the rare coexistence of Ce(III) and Ce(IV) in the MOF structure. The enzymatic studies demonstrated the enzyme-like activities of Ce-BPyDC follow the Michaelis-Menten kinetics and are strongly dependent on temperature, pH, and reaction time. Ce-BPyDC was also revealed to exert high catalytic activity that could transcend horseradish peroxidase and other MOF nanozymes, due to the redox-active Ce(III)/Ce(IV) cycles inside. Furthermore, the simple synthesis, high nanozyme activity, and great stability of Ce-BPyDC motivated us to establish a colorimetric biosensing platform using 3,3',5,5'-tetramethylbenzidine as a color reagent. Adopting this strategy, we established a visual, sensitive, and selective colorimetric method for ascorbic acid (AA) detection, for which the linear interval and limit of detection were 1-20 and 0.28 µM, respectively. The successful AA detection in real juice samples implies the promising use of such mixed-valence MOF nanozymes in food and biomedical samples.


Assuntos
Técnicas Biossensoriais , Cério/química , Colorimetria , Estruturas Metalorgânicas/química , Ácido Ascórbico/análise , Catálise , Concentração de Íons de Hidrogênio , Estruturas Metalorgânicas/síntese química , Temperatura Ambiente
18.
Mar Pollut Bull ; 149: 110511, 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31425847

RESUMO

Microplastics in environments undergo aging processes and may sorb antibiotics from surrounding water. Understanding the interaction between aged microplastics and antibiotics is important to assess the impact of microplastics on environments. In this paper, the sorption of three typical antibiotics, i.e., sulfamethoxazole (SMX), sulfamethazine (SMT), and cephalosporin C (CEP-C) onto the naturally aged microplastics (polystyrene (PS) and polyethylene (PE)) derived from aged plastics samples from the coast of East China Sea and Yellow Sea, China in freshwater and simulated seawater systems were studied. The results indicated that the mixed order (MO) model provided good prediction for the kinetics data. The linear isotherm represented adequately the sorption equilibrium data in freshwater. The Kd values ranged from 0.0236 L·g-1 to 0.0383 L·g-1. In simulated seawater, only CEP-C could be sorbed onto the microplastics. The main sorption mechanisms are hydrophobic, van der Waals, and electrostatic interactions.

19.
Blood Adv ; 3(16): 2499-2511, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31455666

RESUMO

Erythroid maturation requires the concerted action of a core set of transcription factors. We previously identified the Krüppel-type zinc finger transcription factor Zfp148 (also called ZBP-89) as an interacting partner of the master erythroid transcription factor GATA1. Here we report the conditional knockout of Zfp148 in mice. Global loss of Zfp148 results in perinatal lethality from nonhematologic causes. Selective Zfp148 loss within the hematopoietic system results in a mild microcytic and hypochromic anemia, mildly impaired erythroid maturation, and delayed recovery from phenylhydrazine-induced hemolysis. Based on the mild erythroid phenotype of these mice compared with GATA1-deficient mice, we hypothesized that additional factor(s) may complement Zfp148 function during erythropoiesis. We show that Zfp281 (also called ZBP-99), another member of the Zfp148 transcription factor family, is highly expressed in murine and human erythroid cells. Zfp281 knockdown by itself results in partial erythroid defects. However, combined deficiency of Zfp148 and Zfp281 causes a marked erythroid maturation block. Zfp281 physically associates with GATA1, occupies many common chromatin sites with GATA1 and Zfp148, and regulates a common set of genes required for erythroid cell differentiation. These findings uncover a previously unknown role for Zfp281 in erythroid development and suggest that it functionally overlaps with that of Zfp148 during erythropoiesis.

20.
Waste Manag ; 96: 190-197, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31376964

RESUMO

Ionizing radiation coupled with peroxymonosulfate (PMS) oxidation was developed to degrade antibiotics and antibiotic resistance genes (ARGs) from the erythromycin fermentation (EryF) residual wastes. The experimental results showed that the ERY content and ARGs abundance decreased with increase of the absorbed dose and PMS dosage and gamma irradiation was more effective to abate ARGs from the EryF wastes. The removal efficiency of ERY reached 49-55% and more than 96-99% of ARGs (1.32-2.55 log) was eliminated with the absorbed dose of 25-50 kGy and PMS dosage of 50-100 mM. Illumina pyrosequencing revealed that 3 bacterial phyla, Proteobacteria, Firmicutes and Fusobacteria were highly enriched and the ARGs-linked hosts were affiliated to the genera Aeromonas, Enterobacteriaceae and Enterobacter in the phylum Proteobacteria. The abundance of the ARGs-linked bacteria decreased by gamma/PMS treatment. Ionizing radiation/PMS treatment with the doses of 25 kGy and 50 mM PMS is proposed for potential practical application.


Assuntos
Antibacterianos , Eritromicina , Resistência Microbiana a Medicamentos , Fermentação , Genes Bacterianos , Peróxidos
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