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2.
Appl Opt ; 60(23): 7007-7016, 2021 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-34613184

RESUMO

A three-dimensional (3D) measurement method of color fringe projection based on an improved three-step phase-shifting method is proposed. The color fringe pattern is encoded by two cosine fringe patterns with the same frequency but different shifting phase and a uniform gray flat image into three color channels R, G, and B. Although the measurement speed of the traditional three-step phase-shifting method can meet the requirements of measuring 3D objects, it makes the noise and inaccuracy of the captured images increase, and each image will cause measurement error. Therefore, we improve the three-step phase-shifting method and introduce the Hilbert transform into the three-step phase-shift method. The DC component of the fringe pattern is obtained by using the Hilbert transform principle, and the third fringe pattern in the three-step phase-shift method is replaced by the captured light intensity distribution of the DC component. The phase difference of the other two fringe patterns is fixed as π/2 by the Hilbert transform. The improved three-step phase-shifting method is used to obtain the phase information of the deformed color fringe image, and then the phase-unwrapping algorithm is used to obtain the phase distribution information of the whole field. The results show that the improved method can not only accurately calculate the phase information but also greatly improve the measurement speed and quality.

3.
Front Med (Lausanne) ; 8: 685368, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34595184

RESUMO

The study aims to describe the clustering characteristics of Mycobacterium tuberculosis (M.tb) strains circulating in eastern China and determine the ratio of relapse and reinfection in recurrent patients. We recruited sputum smear-positive pulmonary tuberculosis cases from five cities of Jiangsu Province, China, during August 2013 and December 2015. Patients were followed for the treatment outcomes and recurrence based on a cohort design. M.tb strains were isolated and genotyped using the 12-locus MIRU-VNTR. The Beijing family was identified by the extended Region of Difference (RD) analysis. The Hunter-Gaston Discriminatory Index (HGDI) was used to judge the resolution ability of MIRU-VNTR. The odds ratio (OR) together with 95% confidence interval (CI) were used to estimate the strength of association. We performed a cluster analysis on 2098 M.tb isolates and classified them into 545 genotypes and five categories (I, 0.19%; II, 0.43%; III, 3.34%; IV, 77.46%; V, 18.59%). After adjusting for potential confounders, the Beijing family genotype (OR = 118.63, 95% CI: 79.61-176.79, P = 0.001) was significantly related to the dominant strain infections. Patients infected with non-dominant strains had a higher risk of the pulmonary cavity (OR = 1.39, 95% CI: 1.01-1.91, P = 0.046). Among 37 paired recurrent cases, 22 (59.46%) were determined as endogenous reactivation, and 15 (40.54%) were exogenous reinfection. The type of M.tb strains prevalent in Jiangsu Province is relatively single. Beijing family strains infection is dominant in local tuberculosis cases. Endogenous reactivation appears to be a major cause of recurrent tuberculosis in Eastern China. This finding emphasizes the importance of case follow-up and monitoring after the completion of antituberculosis treatment.

4.
BMJ Open ; 11(9): e048324, 2021 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-34593492

RESUMO

BACKGROUND: Accumulating literature has shown the predictive values of inflammation and nutrition-based biomarkers in the prognosis of oesophageal cancer but with inconsistent findings. METHOD: We performed a meta-analysis to systematically evaluate the predictive value of the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), lymphocyte-to-monocyte ratio (LMR), C reactive protein-to-albumin ratio (CAR), systemic inflammation index (SII), prognostic nutritional index (PNI), Glasgow Prognostic Score (GPS) and modified Glasgow Prognostic Score (mGPS) in oesophageal cancer. The outcome indicators include the overall survival (OS), disease-free survival (DFS) and cancer-specific survival (CSS). We applied pooled HR, sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio and area under the curve together with 95% CI to estimate the predictive accuracy. RESULTS: A total of 72 studies, including 22 260 patients, were included in the meta-analysis. Elevated NLR, PLR CAR, SII, GPS, mGPS and decreased LMR and PNI were associated with poor OS of oesophageal cancer. A high level of NLR, PLR and GPS was related to poor DFS. A high level of NLR and GPS was related to poor CSS. The summarised AUC of CAR (0.72, 95% CI: 0.68 to 0.75) and mGPS (0.75, 95% CI: 0.71 to 0.78) surpassed any other indicators. CONCLUSIONS: Clinical indicators such as NLR, PLR, LMR, PNI, SII, CAR, GPS and mGPS have the moderate predictive ability in OS, DFS and CSS of oesophageal cancer. The pretreatment level of CAR and mGPS showed an outstanding prediction value in 5-year OS for oesophageal cancer.

5.
Entropy (Basel) ; 23(9)2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34573809

RESUMO

The setting of the measurement number for each block is very important for a block-based compressed sensing system. However, in practical applications, we only have the initial measurement results of the original signal on the sampling side instead of the original signal itself, therefore, we cannot directly allocate the appropriate measurement number for each block without the sparsity of the original signal. To solve this problem, we propose an adaptive block-based compressed video sensing scheme based on saliency detection and side information. According to the Johnson-Lindenstrauss lemma, we can use the initial measurement results to perform saliency detection and then obtain the saliency value for each block. Meanwhile, a side information frame which is an estimate of the current frame is generated on the reconstruction side by the proposed probability fusion model, and the significant coefficient proportion of each block is estimated through the side information frame. Both the saliency value and significant coefficient proportion can reflect the sparsity of the block. Finally, these two estimates of block sparsity are fused, so that we can simultaneously use intra-frame and inter-frame correlation for block sparsity estimation. Then the measurement number of each block can be allocated according to the fusion sparsity. Besides, we propose a global recovery model based on weighting, which can reduce the block effect of reconstructed frames. The experimental results show that, compared with existing schemes, the proposed scheme can achieve a significant improvement in peak signal-to-noise ratio (PSNR) at the same sampling rate.

6.
Mater Sci Eng C Mater Biol Appl ; 129: 112360, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34579879

RESUMO

Tissue-engineered skin, as a promising skin substitute, can be used for in vitro skin research and skin repair. However, most of research on tissue-engineered skin tend to ignore the rete ridges (RRs) microstructure, which enhances the adhesion between dermis and epidermis and provides a growth environment for epidermal stem cells. Here, we prepared and characterized photocurable gelatin methacrylated (GelMA) and poly(ethylene glycol) diacrylate (PEGDA) co-network hydrogels with different concentrations. Using a UV curing 3D printer, resin molds were designed and fabricated to create three-dimensional micropatterns and replicated onto GelMA-PEGDA scaffolds. Human keratinocytes (HaCaTs) and human skin fibroblasts (HSFs) were co-cultured on the hydrogel scaffold to prepare tissue-engineered skin. The results showed that 10%GelMA-2%PEGDA hydrogel provides the sufficient mechanical properties and biocompatibility to prepare a human skin model with RRs microstructure, that is, it presents excellent structural support, suitable degradation rate, good bioactivity and is suitable for long-term culturing. Digital microscope image analyses showed the micropattern was well-transferred onto the scaffold surface. Both in vitro and in vivo experiments confirmed the formation of the epidermal layer with undulating microstructure. In wound healing experiments, hydrogel can significantly accelerate wound healing. This study provides a simple and powerful way to mimic the structures of human skin and can make a contribution to skin tissue engineering and wound healing.


Assuntos
Gelatina , Hidrogéis , Humanos , Polietilenoglicóis , Pele , Engenharia Tecidual , Tecidos Suporte
7.
Bioengineered ; 2021 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-34565285

RESUMO

Montelukast is a cysteinyl leukotriene receptor 1 (CysLTR1) antagonist widely used to suppress the inflammatory response in asthma and allergic rhinitis. This study aimed to investigate the potential impacts of montelukast on osteoarthritis (OA) progression. To determine the role of montelukast in OA, the expression of CysLTR1 was first examined by quantitative reverse transcription PCR (RT-qPCR) and western blot in IL-1ß-induced ATDC5 cells treated with or without montelukast. Subsequently, the impacts of montelukast on cell viability and oxidative stress were measured by Cell-Counting-Kit-8 (CCK-8), commercial kits and western blot. Oxidative stress-related protein expressions were determined by western blot analysis in Il-1ß-induced ATDC5 cells. Cell apoptosis and cartilage degradation were examined by TdT-mediated dUTP Nick-End Labeling (TUNEL) assay, western blot and RT-qPCR. KLF2 expression was measured in IL-1ß-induced ATDC5 cells treated with montelukast. After interference with small interfering RNA (siRNA)-KLF2 in ATDC5 cells, the loss-of-function assays were also performed in same ways. CysLTR1 expression was elevated in IL-1ß-induced ATDC5 cells but inhibited significantly by montelukast. Montelukast attenuated the oxidative stress and apoptosis, improved cell viability. Moreover, montelukast enhanced KLF2 expression. After transfected with siRNA-KLF2, montelukast attenuated cell injury, oxidative stress, apoptosis and cartilage degradation in IL-1ß-induced ATDC5 cells by activating KLF2.In summary, this work elaborates the evidence that montelukast could attenuate oxidative stress and apoptosis in IL-1ß-induced chondrocytes by inhibiting CysLTR1 and activating KLF2, which can guide the therapeutic strategies of montelukast for OA development in the future.

8.
Chem Commun (Camb) ; 57(76): 9764-9767, 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34486001

RESUMO

In this study, we used lead zirconate titanate (PZT) aerogels prepared by a solvothermal assisted sol-gel method as raw materials to synthesize PZT aerogel/PVDF composite coatings and PZT aerogel sintered sheets through natural annealing and PVDF composite and hot pressing, respectively, and then combined them with the design principle of a biomimetic shell structure to prepare an alternate coating/sheet structured PZT aerogel piezoelectric composite with natural distinguished mechanical properties. It had excellent piezoelectric properties with a piezoelectric coefficient d33 of 435.15 pC N-1 and d31 of -144.55 pC N-1, excellent electromechanical coupling properties with a planar electromechanical coupling coefficient of 60.14%, low dielectric loss of 0.76% at 40 Hz and low density of 3.04 g cm-3. When used as the piezoelectric material in underwater acoustic transducers (UATs), compared with all kinds of piezoelectric ceramics, it achieved higher piezoelectric and comprehensive mechanical properties, lower dielectric loss, lower density, and electromechanical coupling properties similar to that of Pb-containing piezoelectric ceramics, thus showing extremely promising application prospects in UATs.

9.
Environ Pollut ; 290: 117983, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34425372

RESUMO

As a common health indicator in physical examinations, fasting blood glucose (FBG) level measurements are widely applied as a diagnostic method for diabetes mellitus. Uncertain conclusions remained regarding the relationship between PM2.5 exposure and FBG levels. We enrolled 47,471 subjects who participated in annual physical examinations between 2017 and 2019. We collected their general characteristics and FBG levels, and environmental factors simultaneously. We applied the generalized additive model to evaluate the impact of short-term outdoor PM2.5 exposure on FBG levels. Among the entire population, the single-pollutant models showed that a 10 µg/m3 increase in PM2.5 significantly contributed to 0.0030, 0.0233, and 0.0325 mmol/L increases in FBG at lag 0-7 days, lag 0-21 days, and lag 0-28 days, respectively. Accordingly, in multipollutant models, when PM2.5 increased by 10 µg/m3, there was an elevation of 0.0361, 0.0315, 0.0357, and 0.0387 mmol/L in FBG for 8-day, 15-day, 22-day, and 29-day moving averages, respectively. Similarly, we observed a significant positive association between them in the normal population. Moreover, the effects could be modified by age in both the entire and normal populations. Decreasing the ambient PM2.5 concentrations can alleviate the elevation of FBG, which may significantly impact the burden of diabetes mellitus.

10.
J Biochem Mol Toxicol ; 35(9): e22845, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34338401

RESUMO

Diabetic retinopathy (DR) is a common diabetic complication known to cause vision impairment and blindness. Previous studies have demonstrated that proanthocyanidins (PACs), polyphenols that are naturally found in several plants and fruits, have powerful antioxidant and anti-inflammatory effects on various cells. However, the effects and underlying mechanism of PACs against DR pathogenesis remain unknown. Here, we investigated the proliferation, apoptosis, and mechanisms of ARPE-19 cells in response to oxidative stress and inflammation under high-glucose conditions with or without PACs treatment. The Cell-Counting Kit-8 assay and western blot analysis showed that treatment with 10 µl PACs significantly increased cell proliferation and the expression level of Bcl-2 in ARPE-19 cells under high-glucose conditions. Moreover, PACs attenuated the high glucose-induced apoptosis, and the increased expression levels of caspase-3 and Bax. Under high-glucose conditions, the generation of reactive oxygen species (ROS) and levels of malondialdehyde increased, whereas the superoxide dismutase content decreased. Moreover, the expression level of the NLRP3 inflammasome, and the release of interleukin 1ß (IL-1ß) and IL-18 increased. PACs reversed all of these high glucose-induced effects on ARPE-19 cells. Additionally, exposure to nigericin sodium salt, an agonist of the NLRP3 inflammasome, upregulated expression of the NLRP3 inflammasome accompanied by the release of IL-1ß and IL-18. Again, treatment with PACs markedly downregulated these effects. Collectively, these results demonstrate that PACs can prevent retinal pigment epithelial cells from high glucose-induced injury via inhibiting the generation of ROS and activation of the NLRP3 inflammasome, suggesting PACs as a potential candidate for the management of DR.


Assuntos
Células Epiteliais/metabolismo , Glucose/metabolismo , Inflamassomos/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Proantocianidinas/farmacologia , Epitélio Pigmentado da Retina/metabolismo , Linhagem Celular , Retinopatia Diabética/tratamento farmacológico , Retinopatia Diabética/metabolismo , Retinopatia Diabética/patologia , Células Epiteliais/patologia , Humanos , Epitélio Pigmentado da Retina/patologia
11.
Artigo em Inglês | MEDLINE | ID: mdl-34438472

RESUMO

The assembly mechanisms and ecological drivers of abundant and rare bacterial subcommunities in dryland montane forest ecosystems remain unclear. Here, we compared the biogeographic patterns of rare and abundant bacterial subcommunities and examined the ecological drivers governing their assembly processes in a dryland montane forest of China. Our results showed that a stronger relationship existed between phylogenetic turnover and spatial distance in rare subcommunities compared with that in abundant subcommunities. Null model analysis indicated that abundant subcommunities were predominantly controlled by dispersal limitation, whereas variable selection controlled rare bacterial assembly. More importantly, the balance between deterministic and stochastic processes for abundant and rare subcommunities was regulated by soil pH and soil moisture content, respectively, rather than aridity. Increasing soil moisture decreased the importance of deterministic processes for rare bacterial assembly. In abundant subcommunities, the dominance of stochastic processes was higher in neutral pH soils. Our findings suggested that divergent assembly mechanisms underlying distinct biogeographic patterns in rare and abundant bacterial subcommunities in dryland montane forests, and the assembly mechanisms of abundant and rare bacterial subcommunities were mediated by differentiated environmental factors. Our study provides a new understanding of the generation and maintenance of soil biodiversity in dryland ecosystems.

12.
Entropy (Basel) ; 23(8)2021 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-34441142

RESUMO

An adaptive rate Compressive Sensing (CS) method for video signals is proposed. The Blocked Compressive Sensing (BCS) scheme is adopted in this method. Firstly, each video frame is blocked and measured by the BCS scheme, and then the mean and variance of each image block are estimated by observing the CS measurement results. Using the mean and variance of each image block, the sparsity of the block is estimated and then the block can be classified. Adaptive rate sampling is realized by assigning different sampling rates to different classes. At the same time, in order to make better use of the correlation between video frames, a reference block subtraction method is also designed in this paper, which uses the estimates of the sparsity of image blocks as the basis for the reference block update. All operations of the proposed method only depend on the CS measurement results of image blocks and all calculations are simple. Thus, the proposed method is suitable for implementation in CS sampling devices with limited computational performance. Experiment results show that, compared with the actual values, the sparsity estimates and block classification results of the proposed method are accurate. Compared with the latest adaptive Compressive Video Sensing methods, the reconstructed image quality of the proposed method is better.

13.
Exp Cell Res ; 407(1): 112786, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34411608

RESUMO

Activation of Tenon's capsule fibroblasts limits the success rate of glaucoma filtration surgery (GFS), the most efficacious therapy for patients with glaucoma. Angiotensin type 1 receptor (AGTR1) is involved in tissues remodeling and fibrogenesis. However, whether AGTR1 is involved in the progress of fibrogenesis after GFS is not fully elucidated. The aim of this study was to investigate the role of an AGTR1 in scar formation after GFS and the potential anti-fibrosis effect of AGTR1 blocker. AGTR1 expression level was increased in subconjunctival tissues in a rat model of GFS and transforming growth factor-beta 2 (TGF-ß2)-induced human Tenon's capsule fibroblasts (HTFs). AGTR1 blocker treatment suppressed TGF-ß2-induced HTF migration and α-smooth muscle actin (α-SMA) and fibronectin (FN) expression. AGTR1 blocker treatment also attenuated collagen deposition and α-SMA and FN expression in subconjunctival tissues of the rat model after GFS. Moreover, AGTR1 blocker decreased TGF-ß2-induced P65 phosphorylation, P65 nuclear translocation, and nuclear factor kappa B (NF-κB) luciferase activity. Additionally, BAY 11-7082 (an NF-κB inhibitor) significantly suppressed HTF fibrosis. In conclusion, our results indicate that AGTR1 is involved in scar formation after GFS. The AGTR1 blocker attenuates subconjunctival fibrosis after GFS by inhibiting the NF-κB signaling pathway. These findings indicate that targeting AGTR1 is a potential approach to attenuate fibrosis after GFS.

14.
Sci Total Environ ; 793: 148621, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34328976

RESUMO

BACKGROUND: Most studies on associations between meteorological factors and tuberculosis (TB) were conducted in a single city, used different lag times, or merely explored the qualitative associations between meteorological factors and TB. Thus, we performed a multicenter study to quantitatively evaluate the effects of meteorological factors on the risk of pulmonary tuberculosis (PTB). METHODS: We collected data on newly diagnosed PTB cases in 13 study sites in Jiangsu Province between January 1, 2014, and December 31, 2019. Data on meteorological factors, air pollutants, and socioeconomic factors at these sites during the same period were also collected. We applied the generalized additive mixed model to estimate the associations between meteorological factors and PTB. RESULTS: There were 20,472 newly diagnosed PTB cases reported in the 13 study sites between 2014 and 2019. The median (interquartile range) weekly average temperature, weekly average wind speed, and weekly average relative humidity of these sites were 17.3 °C (8.0-24.1), 2.2 m/s (1.8-2.7), and 75.1% (67.1-82.0), respectively. In the single-meteorological-factor models, for a unit increase in weekly average temperature, weekly average wind speed, and weekly average relative humidity, the risk of PTB decreased by 0.9% [lag 0-13 weeks, 95% confidence interval (CI): -1.5, -0.4], increased by 56.2% (lag 0-16 weeks, 95% CI: 32.6, 84.0) when average wind speed was <3 m/s, and decreased by 28.1% (lag 0-14 weeks, 95% CI: -39.2, -14.9) when average relative humidity was ≥72%, respectively. Moreover, the associations remained significant in the multi-meteorological-factor models. CONCLUSIONS: Average temperature and average relative humidity (≥72%) are negatively associated with the risk of PTB. In contrast, average wind speed (<3 m/s) is positively related to the risk of PTB, suggesting that an environment with low temperature, relatively high wind speed, and low relative humidity is conducive to the transmission of PTB.


Assuntos
Conceitos Meteorológicos , Tuberculose Pulmonar , China/epidemiologia , Humanos , Umidade , Incidência , Temperatura , Tuberculose Pulmonar/epidemiologia , Vento
15.
Zhongguo Zhong Yao Za Zhi ; 46(11): 2788-2797, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34296577

RESUMO

NRT1 family proteins play an important roles for absorbing and transporting of nitrate in different plants. In order to identify the NRT1 family genes of Rehmannia glutinosa, this study used 11 NRT1 homologous proteins of Arabidopsis as probe sequences and aligned with the transcriptome data of R. glutinosa by using NCBI BLASTN software. Resulting there were 18 NRT1 proteins were identified in R. glutinosa. On basis of this, a series of the molecular characteristics of R. glutinosa NRT1 proteins including the conserved domains, the transmembrane structure, the subcellular location and phylogenetic features were in detail analyzed. At same time, it were systematically analyzed that the temporal and spatial expression patterns and characteristics of R. glutinosa NRT1 family genes in response to different stress factors. The results indicated that 18 R. glutinosa NRT1 family genes with the length of coding region from 1 260 bp to 1 806 bp, encoded proteins ranging from 419 to 601 amino acids, and all of they owned the domains of typical peptide transporter with 7 to 12 transmembrane domains. These R. glutinosa NRT1 family proteins mostly were found to locate on cellular plasma membrane, and belonged to the hydrophobic proteins. Furthermore, the evolutionary analysis found that the 18 R. glutinosa NRT1 protein family could be divided into two subfamilies, of which 14 NRT1 family genes might occur the positive selection, and 4 genes occur the passivation selection during the evolution process of R. glutinosa. In addition the expression analysis showed that 18 R. glutinosa NRT1 family genes have the distinct expression patterns in different tissues of R. glutinosa, and their expression levels were also obvious difference in response to various stress. These findings infield that 18 R. glutinosa NRT1 family proteins might have obviously different functional roles in nitrate transport of R. glutinosa. In conclusion, this study lays a solid theoretical foundation for clarifying the absorption and transport molecular mechanism of N element during R. glutinosa growth and development, and at same time for deeply studying the molecular function of R. glutinosa NRT1 proteins in absorption and transport of nitrate.


Assuntos
Rehmannia , Proteínas de Transporte de Ânions , Proteínas de Membrana Transportadoras , Nitratos , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Rehmannia/genética , Transcriptoma
16.
BMC Cancer ; 21(1): 840, 2021 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-34284743

RESUMO

BACKGROUND: The objective of this study was to investigate the survival outcomes of surgical margin width in intrahepatic cholangiocarcinoma (ICC). METHODS: Between November 2011 and August 2017, patients who underwent hepatectomy for ICC were collected from 13 major hepatopancreatobiliary centers in China. The survival outcomes for patients who underwent wide margin hepatectomy (WMH) were compared with those who underwent narrow margin hepatectomy (NMH) using the 1:1 propensity score matching (PSM). RESULTS: Among 478 included patients, 195 (40.8%) underwent WMH whereas 283 (59.2%) underwent NMH. PSM yielded 79 matched patients with similar baseline characteristics. Patients underwent WMH had a significant better OS and DFS compared with those underwent NMH (before PSM: median OS 27 vs 17 months, P < 0.05; median DFS 15 vs 8 months, P = 0.001, after PSM: median OS 41 vs 22 months, p < 0.05; median DFS 16 vs 10 months, p < 0.05). However, subgroup analysis based on the AJCC staging system, WMH could only improve the survival outcomes in AJCC I ICC patients (Stage I: OS, DFS, P<0.05). CONCLUSIONS: Surgeons should strive to achieve a wide surgical margin for patients with AJCC I ICC to optimize the long-term outcome.

17.
Nat Commun ; 12(1): 3371, 2021 06 07.
Artigo em Inglês | MEDLINE | ID: mdl-34099671

RESUMO

The role of p53 in tumor suppression has been extensively studied and well-established. However, the role of p53 in parasitic infections and the intestinal type 2 immunity is unclear. Here, we report that p53 is crucial for intestinal type 2 immunity in response to the infection of parasites, such as Tritrichomonas muris and Nippostrongylus brasiliensis. Mechanistically, p53 plays a critical role in the activation of the tuft cell-IL-25-type 2 innate lymphoid cell circuit, partly via transcriptional regulation of Lrmp in tuft cells. Lrmp modulates Ca2+ influx and IL-25 release, which are critical triggers of type 2 innate lymphoid cell response. Our results thus reveal a previously unrecognized function of p53 in regulating intestinal type 2 immunity to protect against parasitic infections, highlighting the role of p53 as a guardian of immune integrity.


Assuntos
Imunidade Inata/imunologia , Intestinos/imunologia , Nippostrongylus/imunologia , Doenças Parasitárias/imunologia , Tritrichomonas/imunologia , Proteína Supressora de Tumor p53/imunologia , Animais , Linhagem Celular Tumoral , Eosinófilos/imunologia , Eosinófilos/parasitologia , Regulação da Expressão Gênica , Células Caliciformes/imunologia , Células Caliciformes/parasitologia , Interações Hospedeiro-Parasita/imunologia , Humanos , Intestino Delgado/imunologia , Intestino Delgado/metabolismo , Intestino Delgado/parasitologia , Intestinos/parasitologia , Proteínas de Membrana/genética , Proteínas de Membrana/imunologia , Proteínas de Membrana/metabolismo , Camundongos Endogâmicos C57BL , Camundongos Knockout , Nippostrongylus/fisiologia , Doenças Parasitárias/metabolismo , Doenças Parasitárias/parasitologia , Tritrichomonas/fisiologia , Proteína Supressora de Tumor p53/genética , Proteína Supressora de Tumor p53/metabolismo
18.
BMC Public Health ; 21(1): 1138, 2021 06 14.
Artigo em Inglês | MEDLINE | ID: mdl-34126966

RESUMO

BACKGROUND: Mental health is affected by both genetic and environmental factors. However, previous studies have showed conflict findings about the role of lifestyle and little is known about the situation of the Chinese population. The purpose of this study was to explore the relationship between the frequency of food consumption, physical exercise condition and mental health, as well as factors related to mental stress in Chinese. METHODS: We recruited 8160 residents who had health examinations in a public hospital during June 2016 to May 2018. Demographic characteristics, the frequency of food consumption, physical exercise condition and mental health status was collected by a questionnaire. We estimated the association using the odds ratio (OR) and 95% confidence interval (CI) based on Binary or ordinal logistic regression models. A classification and regression tree (CART) demonstrated the prediction of the value of a target variable based on other values. RESULTS: The logistic regression model and classification tree model both found that the frequency of fresh vegetables or fruit and fried foods consumption and the current state of drinking alcohol were related to mental stress. The degree of mental stress reduced significantly with increasing consumption of fish (OR = 0.80, 95% CI: 0.71-0.90) and regular exercise (OR = 0.55, 95% CI:0.48-0.64) in females and increased consumption of fish (OR = 0.55, 95% CI: 0.48-0.64) and cereal crop (OR = 0.77, 95% CI: 0.68-0.89), fish (OR = 0.87, 95%CI:0.77-0.96) and regular exercise (OR = 0.61, 95%CI:0.53-0.70) in males. On the contrary, the frequency of consumption of desserts (OR = 1.43, 95% CI: 1.26-1.62) and the current of drinking alcohol (OR = 1.47, 95%CI:1.21-1.79) in females and meat (OR = 1.47, 95%CI: 1.31-1.65), pickled and smoked food (OR = 1.18, 95%CI:1.05-1.32) and the current state of drinking alcohol (OR = 1.25, 95%CI:1.12-1.40) in males were related to an increased risk of mental health. CONCLUSIONS: Our study showed that both the frequency of some food consumption and physical exercise condition were associated with mental health and affected the degree of stress, which provided novel insights into interventions.


Assuntos
Dieta , Verduras , Animais , China/epidemiologia , Estudos Transversais , Exercício Físico , Comportamento Alimentar , Feminino , Humanos , Masculino , Inquéritos e Questionários
19.
STAR Protoc ; 2(2): 100570, 2021 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-34136835

RESUMO

G2/M DNA synthesis (G-MiDS) can be observed in one in five G2/M cells in unperturbed conditions by immunofluorescence microscopy. However, little is known of the genomic sites undergoing G-MiDS. Here, we describe a protocol which allows enriching for G2/M cells and investigating the sites of G-MiDS using BrdU-seq. This method can also be used to study the role of DNA replication or transcription-associated factors in affecting G-MiDS levels in different cell lines. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).

20.
J Biochem Mol Toxicol ; 35(8): e22815, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34047419

RESUMO

Oxidative stress of retinal ganglion cells (RGCs) has been established as a main contributor to retinal degeneration in the pathogenesis of glaucoma. Polo-like kinase 2 (PLK2) has recently been reported to be a potent antioxidant protein that enhances cell survival in response to oxidative stress. To date, the involvement of PLK2 in RGC-associated oxidative stress is undermined. In the present work, we evaluated whether PLK2 regulates oxidative stress evoked by hydrogen peroxide (H2 O2 ) in RGCs. PLK2 expression was induced by H2 O2 stimulation in RGCs. Upregulation of PLK2 had a profoundly cytoprotective effect on H2 O2 -stimulated RGCs by attenuating cellular apoptosis and reactive oxygen species (ROS) level. Further data revealed that upregulation of PLK2 strikingly enhanced the activation of Nrf2 signaling. Moreover, PLK2 overexpression promoted glycogen synthase kinase (GSK)-3ß phosphorylation, whereas PLK2 knockdown reduced the levels of GSK-3ß phosphorylation. Notably, GSK-3ß inhibition using a chemical inhibitor markedly abrogated the suppressive effects of PLK2 knockdown on Nrf2 activation. Repression of Nrf2 blocked the PLK2 overexpression-induced protective effects in H2 O2 -stimulated RGCs. Overall, this study elucidates that upregulation of PLK2 protects RGCs against H2 O2 -induced oxidative stress injury by upregulating Nrf2 activation via modulation of GSK-3ß phosphorylation. These findings underline the pivotal role of PLK2 in mediating oxidative stress-evoked retinal degeneration in the pathogenesis of glaucoma.


Assuntos
Glicogênio Sintase Quinase 3 beta/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Estresse Oxidativo , Proteínas Serina-Treonina Quinases/metabolismo , Células Ganglionares da Retina/metabolismo , Transdução de Sinais , Animais , Linhagem Celular , Peróxido de Hidrogênio , Ratos
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