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1.
Front Vet Sci ; 9: 839926, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35558883

RESUMO

This study examined the effects of soluble fiber (SF) supplementation (0.8%), containing 17.4% rhamnose, 4.1% fucose, 11.1% arabinose, 30.6% xylose, and 16.4% galactose during the prepubescent phase on the subsequent performance from the second to fifth parities. After the first parity, 56 and 55 post-weaning sows in the control (CON) and SF groups had their reproductive performance monitored in succeeding parities. Circulating concentrations of anti-mullerian hormone (AMH) were greater in the SF group than in the CON group at 205 d of age and the first post-weaning day (p < 0.05). The SF treatment at the prepubescent phase resulted in an enhanced reproductive performance from parities three to five. In Parity three, the SF sows had 1.32 total born (p = 0.044), 1.43 born alive (p = 0.023) and 1.40 born effective, which was significantly more than in the CON group (p = 0.022). In Parity four, the SF sows had 1.1 total born (p = 0.058), 1.28 born alive (p = 0.019), and 1.06 born effective, significantly more than in the CON group (p = 0.049). In Parity five, the SF gilts had 1.43 total born (p = 0.075), 1.53 born alive (p = 0.067) and 1.65 born effective, significantly more than in the CON group (p = 0.020). No effects were observed for the removal of sows and backfat thickness at the mating in each parity between groups (p > 0.05). Collectively, gilts that received an extra 0.8% SF during the prepubescent phase increased their subsequent litter size as breeding sows. These results showed that nutritional decisions at the replacement phase could influence lifetime fertility.

2.
Poult Sci ; 101(6): 101886, 2022 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-35526444

RESUMO

Resveratrol (RV) is associated with protection against oxidative stress to improve health, however the effect of RV in layers under oxidative stress (OS) is limited. The objective of this experiment was to investigate the negative effect of OS and protective effects of RV against OS in laying hens. 40 Lohmann layers (25-wk-old; BW = 1.44±0.10 kg) were allocated to four treatments in a 2 × 2 factorial arrangement with either RV (0 or 600 mg/kg) or intraperitoneal injection of tert-butyl hydroperoxide (tBHP) (0 or 800 µmol/kg BW) for 31 days. The results shown that the hens challenged with tBHP presented lower egg-laying rate, feed intake, feed efficiency and higher defective egg rate (P(tBHP)<0.05). The RV were also observed to attenuated egg laying rate and feed intake reduction together with decreased broken egg rate under t-BHP challenge (P(Interaction)≤0.01). The tBHP challenged layer demonstrated lower intestinal morphology (villus height in duodenum and jejunum), lower antioxidant enzymes activities [total superoxidase (SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC)], and glutathione (GSH) levels and higher malondialdehyde (MDA) level] (P(tBHP)<0.05). Dietary RV increased jejunal SOD, GSH-Px and T-AOC activities, and reduced MDA concentration (P(RV) ≤0.05). Layers under tBHP challenge up-regulated mRNA expression of pro-inflammatory cytokine [interleukin-1ß (IL-1ß), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α)] and nuclear factor NF-κB (P(tBHP)<0.05) in jejunum. Dietary RV supplementation down-regulated mRNA gene expression of IL-1ß, IL-6, TNF-α and NF-κB (P(RV) ≤0.05). Dietary RV up-regulated mRNA expression of jejunal barrier-related proteins (claudin-1, claudin-2, mucin-1, and occludin) and ovarian reproductive hormone receptor [steroidogenic acute regulatory protein (StAR), androgen receptor (AR), estrogen receptor 1 (ESR1), and activin a receptor type 1 (ACVR1)] (P(RV) ≤0.05). Overall, the results indicate that tBHP induced oxidative stress to result in reducing production performance, intestinal health and induced ovarian inflammation; whereas dietary RV was able to maintain intestinal health and mitigate the negative impact of tBHP challenge on production performance and ovarian function.

3.
J Chem Phys ; 156(19): 194305, 2022 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-35597629

RESUMO

Steady-state and time-resolved infrared (IR) studies of cyclotetramethylene tetranitramine (HMX) were carried out, using the asymmetric nitro-stretch as probe, to investigate its solution structures and vibrational energy transfer processes in pure dimethyl sulfoxide (DMSO) and in a DMSO/water mixture. A linear IR spectrum in the nitro-stretching mode region shows two major bands and one minor band in DMSO but changes to the two major bands mainly picture when adding water as an antisolvent of HMX, suggesting a transition from well-solvated and less perfect ß-conformation to a less-solvated and close-to-perfect ß-conformation. The latter bears a similar asymmetric nitro-stretch vibration profile to the ß-polymorph in the crystal form. Density functional theory computations of the nitro-stretching vibrations suggest that HMX in DMSO may be in a NO2 group rotated ß-conformation. Two-dimensional IR cross-peak intensity reveals intramolecular energy transfer between the axial and equatorial nitro-groups in the ß-HMX on the ps time scale, which is slightly faster in the mixed solvent case. The importance of water as an antisolvent in influencing the equilibrium solvation structure, as well as the vibrational and orientational relaxation dynamics of HMX, is discussed.


Assuntos
Dimetil Sulfóxido , Vibração , Azocinas , Dimetil Sulfóxido/química , Solventes/química , Água/química
4.
BMC Med ; 20(1): 155, 2022 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-35513832

RESUMO

BACKGROUND: Anlotinib, an oral small molecule tyrosine kinase inhibitor targeting VEGFR 1/2/3, FGFR 1-4, PDGFR a/ß, and c-kit, had demonstrated prolonged progression-free survival (PFS) in refractory metastatic colorectal cancer (mCRC). This multicenter, single-arm, phase II, exploratory study was conducted to evaluate the efficacy and safety of anlotinib combined with capecitabine and oxaliplatin as first-line treatment for unresectable RAS/BRAF wild-type mCRC. METHODS: Patients aged 18-75 with RAS/BRAF wild-type unresectable mCRC, without prior systemic treatment, and ECOG performance status ≤1 were enrolled. Eligible patients received capecitabine (850 mg/m2, p.o., bid, on day 1-14 every 21 days), oxaliplatin (130 mg/m2, i.v., on day 1 every 21 days), and anlotinib (12 mg, p.o., qd, on days 1-14 every 21 days) as induction therapy. Following 6 cycles of therapy, patients who achieved response or stable disease received capecitabine and anlotinib as maintenance therapy until tumor progression. The primary endpoint was objective response rate (ORR) according to RECIST (version: 1.1), and the secondary endpoints were PFS, disease control rate (DCR), duration of response (DOR), and safety. RESULTS: Between November 2019 and February 2021, 31 patients were enrolled. One patient was excluded for refusing treatment. The primary endpoint of ORR was 76.7% (95% CI, 57.7-90.1) with 1 patient achieving a complete response and 22 patients partial response. DCR was 93.3% (95% CI, 77.9-99.2). At a median follow-up of 14.1 months (95% CI, 9.9-18.3), median PFS was 11.3 months (95% CI, 7.1-14.1), and DOR was 7.9 months (95% CI, 5.5-12.7). Twenty-five (83.3%) patients experienced grade 3 or 4 treatment-emergent adverse events (TEAEs). No grade 5 TEAE was reported. The most common grade 3 or 4 TEAEs (>10%) were hypertension (15/30; 50%), neutrophil count decreased (8/30; 26.7%), and diarrhea (4/30; 13.3%). A total of 18 (60%) patients had TEAEs that resulted in dose reduction, interruptions, or delays. CONCLUSIONS: Anlotinib combined with capecitabine and oxaliplatin showed considerable ORR, DCR, PFS, and DOR in the first-line therapy of mCRC with manageable toxicity profiles. TRIAL REGISTRATION: ClinicalTrials.gov : NCT04080843.


Assuntos
Adenocarcinoma , Protocolos de Quimioterapia Combinada Antineoplásica , Neoplasias Colorretais , Adenocarcinoma/tratamento farmacológico , Adenocarcinoma/patologia , Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Capecitabina/uso terapêutico , Neoplasias Colorretais/tratamento farmacológico , Neoplasias Colorretais/patologia , Humanos , Indóis , Oxaliplatina/uso terapêutico , Proteínas Proto-Oncogênicas B-raf , Quinolinas , Resultado do Tratamento
5.
Neuropathology ; 2022 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-35434787

RESUMO

Stroke is one of the leading causes of death and disability worldwide. NLRP3 inflammasome has an essential role in the neuropathology of stroke. Recent studies report that shifting the microglial M1 phenotype to the M2 phenotype protects against ischemic stroke. In the present study, the precise effects of Tranilast, a NLPR3 inflammasome inhibitor, on stroke were evaluated. We established a murine model of distal middle cerebral artery occlusion (dMCAO) and administered Tranilast to dMCAO-induced stroke mice. The NLRP3 level, caspase 1 activity, and infarct volume stroke mice were measured. The sensorimotor function, pro-inflammatory cytokine production, and M1/M2 marker expression were measured. The M1 phenotype was induced by treatment of BV2 microglia with lipopolysacharide and interferon γ, and these BV-2 cells were further treated with Tranilast. The expression of CD16 and CD206 was monitored. dMCAO increased the NLRP3 expression and enhanced caspase 1 activity. Tranilast treatment significantly decreased the infarct volume, improved sensorimotor function, and suppressed the production of inflammatory cytokines in stroke mice. Moreover, Tranilast decreased the M1 marker level while promoting the expression of M2 markers. In summary, our findings suggest that Tranilast ameliorates ischemic stroke through stimulating M2 polarization of microglia.

7.
Comput Intell Neurosci ; 2022: 9554396, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35387243

RESUMO

Time synchronization is the basis of coordination and cooperation in underwater acoustic networks. However, because of the propagation delay, node mobility, and Doppler shift, it is impossible to balance the accuracy and energy consumption simply in water. As a promising technology, partial clustering has high convergence and makes breakthroughs in time synchronization. This paper proposes PCDE-Sync, a novel synchronization mechanism with partial clustering and the Doppler effect. Firstly, a clustering method built on the artificial fish swarm algorithm is presented. It models the cluster construction according to fish's preying, swarming, and following behaviors. Secondly, we design a synchronization mechanism to conduct clock correction and compensation by the Doppler effect. Finally, we compare the performance of PCDE-Sync with the most advanced protocols, namely MU-Sync, MM-Sync, and DE-Sync, in terms of the cumulative error after synchronization, the mean square error under different clock skew and that under distinctive node mobility, and energy consumption. The experimental results show that PCDE-Sync makes a trade-off between accuracy and complexity, which does well in solving synchronization issues.


Assuntos
Acústica , Algoritmos , Análise por Conglomerados , Efeito Doppler , Éteres , Bifenilos Policlorados
9.
Opt Express ; 30(8): 13331-13344, 2022 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-35472948

RESUMO

In this paper, a novel adaptive feedback threshold (AFT) based demodulation for mobile visible light communication and positioning (VLCP) integrated system is proposed. The AFT can vary with the received signal without excessive fluctuation and support communication and positioning in mobile environment. For the VLCP integrated system with single light emitting diodes (LED) and multiple photodetectors (PDs), maximal ratio combining (MRC) and received optical power ratio (ROPR) based on the AFT is further achieved for communication and positioning with high performance, respectively. It is demonstrated that high credible communication and high accuracy positioning for the mobile integrated VLCP system can be realized by using the proposed AFT based demodulation. As a result, the implemented VLCP system with a moving speed of 1 m/s is evaluated experimentally. Average positioning error of 3.43 cm with 800 mA current and the bit error ratio (BER) with different currents are also obtained.

10.
Anal Bioanal Chem ; 414(14): 4235-4244, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35449469

RESUMO

Chemical sensing for the sensitive and reliable detection of mercury(II) ions (Hg2+) is of great importance in environmental protection, food safety, and biomedical applications. Due to the bio-enrichment property of Hg2+ in organisms, it is particularly meaningful to develop an effective tool that can in situ and rapidly monitor the level of Hg2+ in living organisms. In this work, we report ligand functionalized gold-silver bimetallic nanoclusters with bright red fluorescence as intracellular probes for imaging Hg2+ in living cells and zebrafish. The bimetallic nanoclusters of DTT-GSH@Au/AgNCs (DG-Au/AgNCs) with strong fluorescence that benefited from the synergistic effect of Au and Ag atoms were obtained through a one-pot synthesis method, incorporating glutathione (GSH) and dithiothreitol (DTT) as the reducers and functionalized ligands. Attractively, the bright red fluorescence of DG-Au/AgNCs could be rapidly and selectively quenched by Hg2+ within 1 min with a very low detection limit of 1.01 nM. Additionally, DG-Au/AgNCs had a great advantage in the detection of Hg2+ in living cells and zebrafish owing to its notably strong red fluorescence at 665 nm, which could avoid effectively auto-fluorescence interference from the organism. Such easily prepared bimetallic fluorescent nanoclusters would be expected to provide a noninvasive and sensitive approach in the detection of heavy metals in situ for environmental protection.


Assuntos
Mercúrio , Nanopartículas Metálicas , Animais , Glutationa , Ouro/química , Íons/química , Nanopartículas Metálicas/química , Prata/química , Espectrometria de Fluorescência/métodos , Peixe-Zebra
11.
Eur J Psychotraumatol ; 13(1): 2057674, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35401947

RESUMO

Background: Chinese shidu parents (bereaved parents over the age of 49 who have lost their only child) are potentially at a high risk of prolonged grief disorder (PGD), posttraumatic stress disorder (PTSD) and insomnia. Objective: The current study aimed to estimate three network models in 310 shidu parents who met the ICD-11 criteria for PGD: (1) a PGD network to identify central symptoms; (2) a comorbidity network to explore bridge symptoms between PGD and PTSD; (3) a comorbidity network to examine the associations between PGD and insomnia symptoms. Methods: The R-packages bootnet, qgraph and networktools were used to investigate the structure of network models and centrality indices of symptoms. In addition, robustness and significance analyses for the edge weights and the order of centrality were performed. Results: Emotional pain and numbness emerged as the most central symptoms in the PGD network. In the PGD-PTSD comorbidity network, the highest bridge strength symptoms were inability to trust others (PGD) and feeling upset (PTSD). Inability to trust others (PGD), avoidance (PGD), and impairment of life quality (insomnia) were possible bridge symptoms connecting PGD and insomnia. Conclusions: Reducing emotional pain and numbness may be a viable target in PGD interventions for shidu parents. Additionally, findings suggest that future studies could examine the role of inability to trust others and avoidance in PGD comorbidities. HIGHLIGHTS: • Emotional pain and numbness were the most influential symptoms in shidu parents with PGD. The role of PGD symptoms of inability to trust others and avoidance in the comorbidities of PGD with PTSD and insomnia might be worthy of further study.


Antecedentes: Los padres chinos shidu (padres en duelo mayores de 49 años que han perdido a su único hijo) tienen un alto riesgo potencial de presentar trastorno de duelo prolongado (TDP), trastorno de estrés postraumático (TEPT) e insomnio. Objetivo: El presente estudio tuvo como objetivo estimar tres modelos de redes en 310 padres shidu que cumplieron con los criterios CIE-11 para TDP: (1) una red TDP para identificar síntomas centrales; (2) una red de comorbilidad para explorar los síntomas puente entre TDP y TEPT; (3) una red de comorbilidad para examinar las asociaciones entre el TDP y síntomas del insomnio. Métodos: Se utilizaron los paquetes R bootnet, qgraph y networktools para investigar la estructura de los modelos de redes y los índices de centralidad de los síntomas. Además, se realizaron análisis de robustez y significancia para los pesos de borde y el orden de centralidad. Resultados: El dolor y el embotamiento emocional surgieron como los síntomas más centrales en la red TDP. En la red de comorbilidad TDP-TEPT, los síntomas fuerza de puente más altos fueron la incapacidad para confiar en los demás (TDP) y sentirse molesto (TEPT). La incapacidad para confiar en los demás (TDP), la evitación (TDP) y el deterioro de la calidad de vida (insomnio) fueron posibles síntomas puente que conectan el TDP y el insomnio. Conclusiones: Reducir el dolor y el embotamiento emocional puede ser un objetivo viable en las intervenciones de TDP para padres shidu. Además, los hallazgos sugieren que los estudios futuros podrían examinar el papel de la incapacidad para confiar en los demás y la evitación en las comorbilidades del TDP.


Assuntos
Distúrbios do Início e da Manutenção do Sono , Transtornos de Estresse Pós-Traumáticos , Criança , China , Estudos Transversais , Feminino , Humanos , Hipestesia , Masculino , Pessoa de Meia-Idade , Dor , Pais/psicologia , Distúrbios do Início e da Manutenção do Sono/epidemiologia , Transtornos de Estresse Pós-Traumáticos/diagnóstico
12.
Open Life Sci ; 17(1): 202-207, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35415242

RESUMO

Gestational diabetes mellitus (GDM) is a common obstetric disease among pregnant women. This study aimed to explore the effect of dietary pattern management to reduce the incidence of GDM. Here, we, retrospectively, analyzed the influence of dietary patterns on the occurrence of GDM and the correlation between dietary patterns and intestinal microbiome distribution and inflammation in pregnant women. Moreover, patients were assigned to the dietary pattern management group and the nondietary pattern management group, and the effects of dietary patterns on the intestinal microbiome distribution and inflammatory factors were investigated. We found that the intestinal microbiome was changed in GDM patients compared with the healthy controls. The relative abundance of probiotics Lactobacillus and Bifidobacterium significantly decreased in patients with GDM. Moreover, compared with the control group, the expression levels of interleukin-6 and tumor necrosis factor-α were significantly increased. Furthermore, dietary pattern management led to intestinal microbiome changes in patients with GDM. In conclusion, dietary pattern management could alleviate GDM via affecting the intestinal microbiome as well as inflammatory conditions in patients.

13.
Front Vet Sci ; 9: 830392, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35359671

RESUMO

To study the effects of energy and dietary fiber on breast development in gilts and its possible mechanisms, 32 gilts (Landrace × Yorkshire) were randomly allocated into a 2 × 2 factorial design to receive a diet with low or high energy [LE: 33.37 MJ/d digestible energy (DE); HE: 41.87 MJ/d DE] and low or high fiber (LF: 0.3 kg/d dietary fiber, HF: 0.6 kg/d dietary fiber). The weight of breast tissue was recorded. The mammary glands were collected for further analyses. The high energy intake increased the relative weight of breast tissue (p < 0.05) and the content of breast fat (p < 0.05). At the same time, the oil red staining of breast slices also showed an increase in breast fat content in high-energy treatment. High energy intake increased the DNA concentration in breast tissues (p < 0.05). In addition, high energy intake increased the concentration of triglycerides, free fatty acids, and total cholesterol in the blood of gilts (p < 0.05), and the supplementation of high fiber tended to reduce free fatty acids, total cholesterol, and estradiol (p < 0.1). Proteomic analysis suggested that there were notable differences in the cytoskeleton, intracellular non-membrane-bounded organelle, apoptosis, receptor activity, and endopeptidase inhibitor activity in molecular function between the energy and fiber effects (p < 0.05). High fiber intake also decreased the mRNA expression of 5-HT7, Bax, and caspase-3 in the breast tissue of gilts (p < 0.05), which further confirmed the importance of fiber in regulating breast development in gilt. Our results indicate that increasing gilt energy intake improved breast weight and fat deposition and increased breast cell apoptosis. Increased fiber intake reduced breast fat deposition and breast cell apoptosis at high energy intake in gilts. These results provide a potential strategy for dietary intervention against high energy intake in gilts and even in humans.

14.
Int J Biol Sci ; 18(4): 1677-1694, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35280699

RESUMO

Tumor necrosis factor α stimulated gene 6 (TSG-6), a 30-KD secretory protein, plays an essential role in modulating inflammatory responses and extracellular matrix remodeling. However, little is known regarding the role of TSG-6 in human cancers. Here, we investigated the mechanism of action and the role of TSG-6 in colorectal cancer (CRC) metastasis. We found that TSG-6 was highly expressed in tumor tissues and was associated with poor prognosis and metastasis in CRC. Mechanistically, TSG-6 overexpression in CRC cells resulted in ERK activation and epithelial-mesenchymal transition by means of stabilizing CD44 and facilitating the CD44-EGFR complex formation on the cell membrane. Consequently, this resulted in the promotion of tumor migration and invasion both in vitro and in vivo. Notably, our data showed that CRC cells secreted TSG-6 could trigger a paracrine activation of JAK2-STAT3 signaling and reprogram normal fibroblasts into cancer-associated fibroblasts, which exhibited upregulation of pro-metastatic cytokines (CCL5 and MMP3) and higher movement ability. In animal models, the co-injection of cancer cells and TSG6-reprogrammed fibroblasts led to a significant increase in tumor metastasis. Our findings indicated that TSG-6 overexpression in CRC cells could promote cancer metastasis in both an autocrine and paracrine manner. Therefore, targeting TSG-6 might be a potential therapeutic strategy for the treatment of metastatic CRC.


Assuntos
Fibroblastos Associados a Câncer , Neoplasias Colorretais , Animais , Fibroblastos Associados a Câncer/metabolismo , Linhagem Celular Tumoral , Movimento Celular/genética , Neoplasias Colorretais/metabolismo , Transição Epitelial-Mesenquimal/genética , Fibroblastos/metabolismo , Regulação Neoplásica da Expressão Gênica , Transdução de Sinais/genética , Microambiente Tumoral/genética
15.
Poult Sci ; 101(4): 101720, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35231770

RESUMO

The purpose of this experiment is to explore the effects of dietary supplementation of benzoic acid, Enterococcus faecium, and essential oil complex (BEC) on coccidia and Clostridium perfringens challenge in laying hens. A total of 80 Lohmann gray laying hens (35 wk old) were allocated to 4 treatments in a 2 × 2 factorial arrangement with the main effects of Clostridium perfringens type A (CP) and coccidia challenge (with or without challenge) and 2 BEC levels (0 and 1,000 mg/kg). The total experimental period was 6 wk. The results showed that: the challenge group significantly decreased the laying rate and average daily feed intake (ADFI) of laying hens (PChallenge < 0.01). The BEC + challenge group significantly increased the laying rate and decreased the feed conversion ratio (FCR) of laying hens (PBEC < 0.05). The challenge significantly decreased the thickness, strength, and relative weight of eggshell (PChallenge < 0.05). The BCE + challenge group significantly increased the relative weight and strength of the eggshell (PBEC < 0.05). The challenge significantly increased the crypt depth of the duodenum, jejunum and ileum, and decreased the villus-to-crypt ratio (V/C) (PChallenge < 0.01). The BEC + challenge group decreased the crypt depth of the duodenum and jejunum, and increased the V/C of the duodenum (PBEC < 0.01). The pathological scores of duodenum and jejunum of the challenge group were significantly higher than other groups (PChallenge < 0.01), while the BEC + challenge group had lower pathological scores of jejunum (PBEC < 0.01). The challenge significantly decreased the mRNA expression of Occludin, Mucin-2, Zonula occluden-1 (ZO-1) (Pchallenge < 0.05); whereas the BEC group significantly increased the expression of Occludin, Mucin-2, and Claudin-1 mRNA (PBEC < 0.05). The challenge significantly increased the level of interleukin 1ß (IL-1ß) in the jejunum (PChallenge < 0.05). Taken together, adding BEC to the diet can improved production performance and egg quality of layers, by protecting intestinal health against Clostridium perfringens type A (CP) and coccidia challenge.


Assuntos
Coccídios , Enterococcus faecium , Óleos Voláteis , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Ácido Benzoico , Galinhas , Clostridium perfringens , Dieta/veterinária , Suplementos Nutricionais/análise , Feminino , Mucina-2 , Ocludina , Óleos Voláteis/farmacologia , Óvulo , RNA Mensageiro
16.
Phytochemistry ; 198: 113140, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35245524

RESUMO

Two pairs of undescribed enantiomers including a 2,5-diketopiperazine namely (±)-janthinolide G and a related analogue (±)-janthinolide H, were isolated from the crude extract of the fungus Penicillium griseofulvum together with five known compounds. Both two structures were determined by spectroscopic method and HRESIMS, whereas absolute stereochemistry was elucidated by using theoretical NMR calculation and ECD calculation. Janthinolide G is the first example of 2,5-diketopiperazine enantiomers with a cleavage diketopiperazine ring and comprises a terminal oxime group rarely seen in natural products. Biological screening of selected compounds found that 4 and 7 both exhibited weak α-glucosidase inhibitory effects, and a potential correlation was afforded by docking studies of α-glucosidase protein (PDB: 3TOP) and bioactive molecules. The plausible biosynthetic pathways of two unreported isolates are proposed here.


Assuntos
Penicillium , alfa-Glucosidases , Fungos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Penicillium/química
17.
J Oncol ; 2022: 5758601, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35251173

RESUMO

BACKGROUND: The homeobox (HOX) gene family has been found to be involved in human cancers. However, its involvement in hepatocellular carcinoma (HCC) has not been well documented. Here, we comprehensively evaluated the role of HOXs in HCC. METHODS: RNA sequencing profile of TCGA-LIHC and LIRI-JP were obtained from the Cancer Genome Atlas (TCGA) and the International Cancer Genome Consortium (ICGC), respectively. Data of TCGA-LIHC methylation were downloaded from UCSC Xena. Genetic alteration data for the TCGA samples was obtained from cBioPortal and GSCA. The diagnostic efficiency was assessed using ROC curves. The prognostic significance was evaluated by the Kaplan-Meier method and Cox regression analysis. Subsequent functional analysis was performed through the clusterProfiler package. ssGSEA, ESTIMATE, and TIDE algorithms were employed to explore the relationship between HOXs and the HCC microenvironment. Finally, pRRophetic package and NCI-60 cancerous cell lines were applied to estimate anticancer drug sensitivity. RESULTS: The mRNA levels of HOXs in HCC tissues were higher than those of noncancerous tissues and were correlated with poor overall survival (OS). HOXA6, C6, D9, D10, and D13 could serve as independent risk factors for OS. Further functional analysis revealed that these five HOXs regulate the cell proliferation, cell cycle, immune response, and microenvironment composition of HCC. In addition, the aberrant expression and methylation of HOXs is of great value in the diagnosis of HCC. CONCLUSION: HOXs play crucial roles in HCC and could serve as potential markers for HCC diagnosis and prognosis.

18.
J Control Release ; 345: 586-600, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35351528

RESUMO

A group of nucleic acids and oligonucleotides play various roles in the innate immune system. They can stimulate pattern recognition receptors to activate innate immune cells, encode immunostimulatory proteins or peptides, or silence specific genes to block negative regulators of immune cells. Given the limitations of current cancer immunotherapy, there has been increasing interest in harnessing innate immune responses by nucleic acids and oligonucleotides. The poor biopharmaceutical properties of nucleic acids and oligonucleotides make it critical to use carriers that can protect them in circulation, retain them in the tumor microenvironment, and bring them to intracellular targets. Therefore, various gene carriers have been repurposed to deliver nucleic acids and oligonucleotides for cancer immunotherapy and improve their safety and activity. Here, we review recent studies that employed carriers to enhance the functions of nucleic acids and oligonucleotides and overall immune responses to cancer, and discuss remaining challenges and future opportunities in the development of nucleic acid-based immunotherapeutics.


Assuntos
Neoplasias , Ácidos Nucleicos , Humanos , Imunidade Inata , Imunoterapia , Neoplasias/metabolismo , Oligonucleotídeos/uso terapêutico , Microambiente Tumoral
19.
Exp Ther Med ; 23(4): 250, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35261622

RESUMO

[This retracts the article DOI: 10.3892/etm.2017.4367.].

20.
ACS Appl Mater Interfaces ; 14(10): 12395-12403, 2022 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-35235303

RESUMO

Recently, cuprous halide perovskite-type materials have drawn tremendous attention for their intriguing optical properties. Here, a zero-dimensional (0D) Cu(I)-based compound of [(C3H7)4N]2Cu2I4 ([C3H7)4N]+ = tetrapropylammonium cation) was synthesized by a facile solution method, a monoclinic system of P21/n symmetry with a Cu2I42- cluster as the confined structure. The as-synthesized [(C3H7)4N]2Cu2I4 exhibits bright dual-band pure white emission with a photoluminescence quantum yield (PLQY) of 91.9% and CIE color coordinates of (0.33, 0.35). Notably, this compound also exhibits an ultrahigh color rendering index (CRI) of 92.2, which is comparable to the highest value of single-component metal halides reported recently. Its Raman spectra provide a clear spectral profile of strong electron-phonon interaction after [(C3H7)4N]+ incorporation, favoring the self-trapped exciton (STE) formation. [(C3H7)4N]2Cu2I4 can give dual-STE bands at the same time because of the Cu-Cu metal bond in a Cu2I42- cluster, whose populations could be scaled by temperature, together with the local dipole orientation modulation of neighboring STEs and phase transition related emission color coordinate change. Particularly, the outstanding chemical- and antiwater stability of this compound was also demonstrated. This work illustrates the potential of such cuprous halide perovskite-type materials in multifunctional applications, such as lighting in varied environments.

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