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1.
J Leukoc Biol ; 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34585438

RESUMO

The dysregulation of skin microflora in patients with atopic dermatitis (AD) has become a research hotspot in recent years. Metagenomic studies have shown that microbial diversity is decreased, whereas the Staphylococcus aureus infection is increased in AD. Keratinocytes are the primary barrier against the invasion of external pathogenic microorganisms. Staphylococcus aureus infection can abnormally activate innate and adaptive immune responses in keratinocytes, resulting in a vicious cycle between Staphylococcus aureus infection and AD. This article reviews the mechanisms of inflammatory damage of keratinocytes induced by Staphylococcus aureus infection in patients with AD, providing a theoretical basis for the study of new targeted drugs. This review also suggests for the management of Staphylococcus aureus infection in patients with AD.

2.
Dev Cell ; 56(18): 2592-2606.e7, 2021 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-34508658

RESUMO

Membrane contact between intracellular organelles is important in mediating organelle communication. However, the assembly of molecular machinery at membrane contact site and its internal organization correlating with its functional activity remain unclear. Here, we demonstrate that a gel-like condensation of Cidec, a crucial protein for obesity development by facilitating lipid droplet (LD) fusion, occurs at the LD-LD contact site (LDCS) through phase separation. The homomeric interaction between the multivalent N terminus of Cidec is sufficient to promote its phase separation both in vivo and in vitro. Interestingly, Cidec condensation at LDCSs generates highly plastic and lipid-permeable fusion plates that are geometrically constrained by donor LDs. In addition, Cidec condensates are distributed unevenly in the fusion plate generating stochastic sub-compartments that may represent unique lipid passageways during LD fusion. We have thus uncovered the organization and functional significance of geometry-constrained Cidec phase separation in mediating LD fusion and lipid homeostasis.

3.
Front Immunol ; 12: 628512, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33868246

RESUMO

Background: Patients with atopic dermatitis (AD) exhibit phenotypic variability in ethnicity and IgE status. In addition, some patients develop other allergic conditions, such as allergic rhinitis (AR), in subsequent life. Understanding the heterogeneity of AD would be beneficial to phenotype-specific therapies. Methods: Twenty-eight Chinese AD patients and 8 healthy volunteers were enrolled in the study. High-throughput transcriptome sequencing was conducted on lesional and nonlesional skin samples from 10 AD patients and matched normal skin samples from 5 healthy volunteers. Identification of differentially expressed genes (DEGs), KEGG pathway analyses, and sample cluster analyses were conducted in the R software environment using the DEseq2, ClusterProfiler, and pheatmap R packages, respectively. qRT-PCR, Western blotting, and ELISA were used to detect gene expression levels among subtypes. Correlation analysis was performed to further investigate their correlation with disease severity. Results: A total of 25,798 genes were detected per sample. Subgroup differential expression analysis and functional enrichment analysis revealed significant changes in the IL17 signaling pathway in Chinese EAD patients but not in IAD patients. DEGs enriched in cytokine-cytokine receptor interactions and gland secretion were considered to be associated with atopic march. Further investigations confirmed a marked IL17A upregulation in Chinese EAD with a positive relationship with total IgE level and AD severity. In addition, increased IL17A in AD patients with AR demonstrated a closer association with AR severity than IL4R. Moreover, AQP5 and CFTR were decreased in the lesions of AD patients with AR. The CFTR mRNA expression level was negatively associated with the skin IL17A level and AR severity. Conclusion: Our research characterized marked Th17 activation in Chinese EAD patients, and altered expression of IL17A, IL4R, AQP5, and CFTR in AD patients with AR was associated with AR severity. It partially explained the phenotypic differences of AD subtypes and provided potential references for endotype-targeted therapy.


Assuntos
Regulador de Condutância Transmembrana em Fibrose Cística/genética , Dermatite Atópica/genética , Perfilação da Expressão Gênica , Ativação Linfocitária/genética , RNA-Seq , Células Th17/imunologia , Transcriptoma , Aquaporina 5/genética , Aquaporina 5/metabolismo , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , China/epidemiologia , Regulador de Condutância Transmembrana em Fibrose Cística/metabolismo , Dermatite Atópica/etnologia , Dermatite Atópica/imunologia , Dermatite Atópica/metabolismo , Redes Reguladoras de Genes , Humanos , Interleucina-17/genética , Interleucina-17/metabolismo , Subunidade alfa de Receptor de Interleucina-4/genética , Subunidade alfa de Receptor de Interleucina-4/metabolismo , Fenótipo , Índice de Gravidade de Doença , Células Th17/metabolismo
4.
Q J Exp Psychol (Hove) ; 74(9): 1512-1524, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33783279

RESUMO

Memory is considered to be a flexible and reconstructive system. However, there is little experimental evidence demonstrating how associations are falsely constructed in memory, and even less is known about the role of the self in memory construction. We investigated whether false associations involving non-presented stimuli can be constructed in episodic memory and whether the self plays a role in such memory construction. In two experiments, we paired participants' own names (i.e., self-reference) or the name "Adele" (i.e., other-reference) with words and pictures from Deese/Roediger-McDermott (DRM) lists. We found that (1) participants not only falsely remembered the non-presented lure words and pictures as having been presented, but also misremembered that they were paired with their own name or "Adele," depending on the referenced person of related DRM lists; and (2) there were more critical lure-self associations constructed in the self-reference condition than critical lure-other associations in the other-reference condition for word but not for picture stimuli. These results suggest a self-enhanced constructive effect that might be driven by both relational and item-specific processing. Our results support the spreading-activation account for constructive episodic memory.


Assuntos
Rememoração Mental , Nomes , Humanos , Repressão Psicológica
5.
Environ Sci Pollut Res Int ; 28(28): 38106-38116, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33728606

RESUMO

Rice production consumes more water than the production of other crop species due to the specific growth requirements of this species. Accurately accounting for water consumption during rice production and analyzing the spatio-temporal changes in water consumption are thus necessary. Using the water footprint (WF) as an indicator and combining data from multi-sources, this paper explored the regional differences in rice WFs in Jilin Province at a spatial resolution of 1 km. The results showed that the blue WF was always larger than the green WF, and the total, green and blue WFs were lowest during the humid year. The pixels with high values of total, green and blue WFs were mainly distributed in the eastern region of Jilin Province. Compared with the traditional estimation of the WF based on the data of administrative regions, RS techniques can overcome the administrative boundary and provide near real-time data concerning specific agricultural parameters to extract more accurate results for WF models. The combination of RS data and statistical, observational, and survey data can thus overcome the limitations of weather conditions affecting RS, reduce the incorporation of parameters, and estimate WFs quickly and accurately. This study provides a framework to evaluate crop WFs with multi-source data.


Assuntos
Oryza , Agricultura , China , Água , Abastecimento de Água
6.
J Exp Psychol Gen ; 149(10): 1996-2000, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-33017163

RESUMO

What does believing in repressed memory mean? In a recent article in this journal, Brewin, Li, Ntarantana, Unsworth, and McNeilis (2019, Study 3) argued that when people are asked to indicate their belief in repressed memory, they might actually think of deliberate memory suppression rather than unconscious repressed memory. They further argued that in contrast to belief in unconscious repressed memory, belief in deliberate memory suppression is not scientifically controversial. In this commentary, we show that they are incorrect on both counts. Although Brewin and colleagues surveyed people to indicate their belief in deliberate memory suppression, they neglected to ask their participants whether they (also) believed in unconscious repressed memory. We asked people from the general population whether they believed that traumatic experiences can be unconsciously repressed for many years and then recovered. In 2 studies of the general population, we found high endorsement rates (Study 1 [N = 230]: 59.2% [n = 45]; Study 2 [N = 79]: 67.1% [n = 53]) of the belief in unconscious repressed memory. These endorsement rates did not statistically differ from endorsement rates to statements on repressed memory and deliberate memory suppression. In contrast to what Brewin et al. argued, belief in unconscious repressed memory among lay people is alive and well. Finally, we contend that Brewin et al. overstated the scientific evidence bearing on deliberate repression (suppression). (PsycInfo Database Record (c) 2020 APA, all rights reserved).


Assuntos
Memória , Repressão Psicológica , Humanos
7.
J Exp Psychol Gen ; 149(10): 2005-2006, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-33017165

RESUMO

We show that, in contrast to Brewin, Li, Ntarantana, Unsowrth, and McNeilis (2019), large proportions of laypersons believe in the scientifically controversial phenomenon of unconscious repressed memories. We provide new survey data showing that when participants are asked specific questions about what they mean when they report that traumatic memories can be repressed, most provide answers strongly consistent with unconscious repression. Our findings continue to show that researchers, legal professionals, and clinicians should be wary of invoking unconscious repression in their work. (PsycInfo Database Record (c) 2020 APA, all rights reserved).


Assuntos
Repressão Psicológica , Humanos
8.
Medicine (Baltimore) ; 99(34): e21591, 2020 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-32846767

RESUMO

BACKGROUND: Obstructive sleep apnea hypopnea syndrome (OSAHS) is a common, chronic sleep disease. As the incidence of OSAHS increases, it has seriously threatened people's health. There have been an increasing number of clinical trials of OSAHS in recent years. However, the clinical trials of OSAHS have heterogeneous outcomes, surrogate outcomes, subjective outcomes, and composite outcomes, as well as the lack of endpoints or patient perspectives. The best method is to develop a core outcomes sets (COSs) for OSAHS's clinical trials. METHODS: The development of COSs of OSAHS will include 5 stages: RESULTS:: The results of our study will be published in a peer-reviewed journal. DISCUSSION: The development of the COSs of OSAHS will improve the design and operation of OSAHS clinical trials to conform to international standards and ensure the credibility of the outcomes. In addition, this study will involve different stakeholder groups to help ensure that the developed COSs will be suitable and well accepted. TRIAL REGISTRATION NUMBER: 1544.


Assuntos
Ensaios Clínicos como Assunto/métodos , Apneia Obstrutiva do Sono/terapia , Humanos , Resultado do Tratamento
11.
Mol Immunol ; 120: 23-31, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32045771

RESUMO

Acne is a common chronic inflammatory skin disease, and the inflammation immune response runs through all stages of acne lesions. In this study, we use a combination of multiplex-PCR and high-throughput sequencing technologies to analyze T cell receptor ß chain CDR3 (complementarity-determining region 3) in peripheral blood isolated from severe acne patients. Once compared with healthy controls, we propose to identify acne-relevant CDR3 peptides. Our results reveal that the diversity of T cell receptor ß chain (TRB) CDR3 sequences in the peripheral blood of the severe acne vulgaris (SA) group differed from that of the control group. In addition, we find 10 TRB CDR3 sequences, amino acid sequences and V-J combinations with significantly different expressions between the SA group and the non-acne (NA) group (P < 0.0001). These findings may contribute to a better understanding of the role of immunity in the pathogenesis of acne and may serve as biomarkers for evaluating risk or prognosis of severe acne disease in future.


Assuntos
Acne Vulgar/genética , Acne Vulgar/imunologia , Regiões Determinantes de Complementaridade/genética , Genes Codificadores da Cadeia beta de Receptores de Linfócitos T , Adolescente , Sequência de Aminoácidos , Sequência de Bases , Variação Genética , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , Reação em Cadeia da Polimerase Multiplex , Análise de Sequência de DNA , Análise de Sequência de Proteína , Adulto Jovem
12.
BMJ Open ; 9(9): e023162, 2019 09 08.
Artigo em Inglês | MEDLINE | ID: mdl-31501092

RESUMO

INTRODUCTION: Starting dialysis early or late both result in a low quality of life and a poor prognosis in patients undergoing haemodialysis. However, there remains no consensus on the optimal timing of dialysis initiation, mainly because of a lack of suitable methods to assess variations in dialysis initiation time. We have established a novel equation named DIFE (Dialysis Initiation based on Fuzzy-mathematics Equation) through a retrospective, multicentre clinical cohort study in China to determine the most suitable timing of dialysis initiation. The predictors of the DIFE include nine biochemical markers and clinical variables that together influence dialysis initiation. To externally validate the clinical accuracy of DIFE, we designed the assessment of DIFE (ADIFE) study as a prospective, open-label, multicentre, randomised controlled trial to assess the clinical outcomes among patients who initiate dialysis in an optimal start dialysis group and a late-start dialysis group, based on DIFE. METHODS AND ANALYSIS: A total of 388 enrolled patients with end-stage renal disease will be randomised 1:1 to the optimal start dialysis group, with a DIFE value between 30 and 35, or the late-start dialysis group, with a DIFE value less than 30, using the Randomization and Trial Supply Management system. Participants will be assessed for changes in signs and symptoms, dialysis mode and parameters, biochemical and inflammatory markers, Subjective Global Assessment, Kidney Disease Quality of Life Short Form, Cognitive Assessment, medical costs, adverse events and concomitant medication at baseline, predialysis visiting stage and postdialysis visiting stage, every 12-24 weeks. The following data will be recorded on standardised online electronic case report forms. The primary endpoint is 3-year all-cause mortality. The secondary endpoints include non-fatal cerebrocardiovascular events, annual hospitalisation rate, quality of life, medical costs and haemodialysis related complications. ETHICS AND DISSEMINATION: Ethical approval was obtained from the Ethics Committee of the First Affiliated Hospital of Dalian Medical University China (registration no: YJ-KY-2017-119) and the ethics committees of all participating centres. The final results of the ADIFE trial will be presented to the study sponsor, clinical researchers and the patient and public involvement reference group. Findings will be disseminated through peer-reviewed journals, Clinical Practice Guidelines and at scientific meetings. TRIAL REGISTRATION NUMBER: ClinicalTrial.gov. Registry (NCT03385902); pre-results.


Assuntos
Falência Renal Crônica/terapia , Ensaios Clínicos Controlados Aleatórios como Assunto , Diálise Renal/normas , Tempo para o Tratamento/normas , Adulto , Algoritmos , Estudos de Coortes , Feminino , Lógica Fuzzy , Humanos , Masculino , Estudos Prospectivos , Projetos de Pesquisa
13.
N Engl J Med ; 381(11): 1011-1022, 2019 09 12.
Artigo em Inglês | MEDLINE | ID: mdl-31340116

RESUMO

BACKGROUND: Roxadustat is an oral hypoxia-inducible factor prolyl hydroxylase inhibitor that stimulates erythropoiesis and regulates iron metabolism. Additional data are needed regarding the effectiveness and safety of roxadustat as compared with standard therapy (epoetin alfa) for the treatment of anemia in patients undergoing dialysis. METHODS: In a trial conducted in China, we randomly assigned (in a 2:1 ratio) patients who had been undergoing dialysis and erythropoiesis-stimulating agent therapy with epoetin alfa for at least 6 weeks to receive roxadustat or epoetin alfa three times per week for 26 weeks. Parenteral iron was withheld except as rescue therapy. The primary end point was the mean change in hemoglobin level from baseline to the average level during weeks 23 through 27. Noninferiority of roxadustat would be established if the lower boundary of the two-sided 95% confidence interval for the difference between the values in the roxadustat group and epoetin alfa group was greater than or equal to -1.0 g per deciliter. Patients in each group had doses adjusted to reach a hemoglobin level of 10.0 to 12.0 g per deciliter. Safety was assessed by analysis of adverse events and clinical laboratory values. RESULTS: A total of 305 patients underwent randomization (204 in the roxadustat group and 101 in the epoetin alfa group), and 256 patients (162 and 94, respectively) completed the 26-week treatment period. The mean baseline hemoglobin level was 10.4 g per deciliter. Roxadustat led to a numerically greater mean (±SD) change in hemoglobin level from baseline to weeks 23 through 27 (0.7±1.1 g per deciliter) than epoetin alfa (0.5±1.0 g per deciliter) and was statistically noninferior (difference, 0.2±1.2 g per deciliter; 95% confidence interval [CI], -0.02 to 0.5). As compared with epoetin alfa, roxadustat increased the transferrin level (difference, 0.43 g per liter; 95% CI, 0.32 to 0.53), maintained the serum iron level (difference, 25 µg per deciliter; 95% CI, 17 to 33), and attenuated decreases in the transferrin saturation (difference, 4.2 percentage points; 95% CI, 1.5 to 6.9). At week 27, the decrease in total cholesterol was greater with roxadustat than with epoetin alfa (difference, -22 mg per deciliter; 95% CI, -29 to -16), as was the decrease in low-density lipoprotein cholesterol (difference, -18 mg per deciliter; 95% CI, -23 to -13). Roxadustat was associated with a mean reduction in hepcidin of 30.2 ng per milliliter (95% CI, -64.8 to -13.6), as compared with 2.3 ng per milliliter (95% CI, -51.6 to 6.2) in the epoetin alfa group. Hyperkalemia and upper respiratory infection occurred at a higher frequency in the roxadustat group, and hypertension occurred at a higher frequency in the epoetin alfa group. CONCLUSIONS: Oral roxadustat was noninferior to parenteral epoetin alfa as therapy for anemia in Chinese patients undergoing dialysis. (Funded by FibroGen and FibroGen [China] Medical Technology Development; ClinicalTrials.gov number, NCT02652806.).


Assuntos
Anemia/tratamento farmacológico , Epoetina alfa/uso terapêutico , Glicina/análogos & derivados , Hematínicos/uso terapêutico , Hemoglobinas/análise , Prolina Dioxigenases do Fator Induzível por Hipóxia/antagonistas & inibidores , Isoquinolinas/uso terapêutico , Insuficiência Renal Crônica/complicações , Adulto , Idoso , Análise de Variância , Anemia/etiologia , Colesterol/sangue , Método Duplo-Cego , Epoetina alfa/efeitos adversos , Feminino , Glicina/efeitos adversos , Glicina/uso terapêutico , Hematínicos/efeitos adversos , Humanos , Hiperpotassemia/induzido quimicamente , Hipertensão/induzido quimicamente , Isoquinolinas/efeitos adversos , Masculino , Pessoa de Meia-Idade , Diálise Renal , Insuficiência Renal Crônica/sangue , Insuficiência Renal Crônica/terapia
14.
J Cell Physiol ; 234(12): 23518-23527, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31219186

RESUMO

Melanoma is responsible for the majority of deaths caused by skin cancer. Antitumor activity of microRNA-329 (miR-329) has been seen in several human cancers. In this study, we identify whether miR-329 serves as a candidate regulator in melanoma. Melanoma-related differentially expressed genes were screened with its potential molecular mechanism predicted. Melanoma tissues and pigmented nevus tissues were collected, where the levels of miR-329 and high-mobility group box 2 (HMGB2) were determined. To characterize the regulatory role of miR-329 on HMGB2 and the ß-catenin pathway in melanoma cell activities, miR-329 mimics, miR-329 inhibitors, and siRNA-HMGB2 were transfected into melanoma cells. Cell viability, migration, invasion, cell cycle, and apoptosis were assessed. miR-329 was predicted to influence melanoma by targeting HMGB2 via the ß-catenin pathway. High level of HMGB2 and low miR-329 expression were observed in melanoma tissues. HMGB2 was targeted and negatively regulated by miR-329. In melanoma cells transfected with miR-329 mimics or siRNA-HMGB2, cell proliferation, migration, and invasion were impeded, yet cell cycle arrest and apoptosis were promoted, corresponding to decreased levels of ß-catenin, cyclin D1, and vimentin and increased levels of GSK3ß and E-cadherin. Collectively, our results show that miR-329 can suppress the melanoma progression by downregulating HMGB2 via the ß-catenin pathway.


Assuntos
Glicogênio Sintase Quinase 3 beta/metabolismo , Proteína HMGB2/metabolismo , Melanoma/metabolismo , MicroRNAs/metabolismo , Neoplasias Cutâneas/metabolismo , beta Catenina/metabolismo , Antígenos CD/genética , Antígenos CD/metabolismo , Apoptose , Caderinas/genética , Caderinas/metabolismo , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Ciclina D1/genética , Ciclina D1/metabolismo , Feminino , Regulação Neoplásica da Expressão Gênica , Glicogênio Sintase Quinase 3 beta/genética , Proteína HMGB2/genética , Humanos , Masculino , Melanoma/genética , Melanoma/patologia , MicroRNAs/genética , Pessoa de Meia-Idade , Invasividade Neoplásica , Transdução de Sinais , Neoplasias Cutâneas/genética , Neoplasias Cutâneas/patologia , Vimentina/genética , Vimentina/metabolismo , beta Catenina/genética
15.
J Diabetes Res ; 2019: 5204394, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31218232

RESUMO

Aims: MicroRNAs (miRNAs) stably and abundantly exist in body fluids and have been considered as novel and noninvasive biomarkers for several diseases. The present study is aimed at investigating the expression profiling and clinical significance of plasma miRNAs in the pathogenesis and progression of diabetic nephropathy (DN). Methods: Plasma samples were obtained from 66 DN patients (36 had microalbuminuria and 30 had macroalbuminuria), 36 diabetic patients with normoalbuminuria, and 40 healthy controls. The plasma miRNA profiles were obtained by miRNA low-density array chip and validated by quantitative real-time polymerase chain reaction. The correlations between the differential expression of plasma miRNAs and clinicopathological parameters were explored. Results: miR-150-5p, miR-155-5p, miR-30e, miR-320e, and miR-3196 were found to be differentially expressed in plasma samples among these three groups: diabetic patients with microalbuminuria, diabetic patients with normoalbuminuria, and healthy controls (P < 0.05). The expression levels of miR-150-5p and miR-155-5p in patients with macroalbuminuria were 2.3-fold (P = 0.001) and 1.5-fold (P = 0.033) higher than patients with microalbuminuria, respectively. However, the expression levels of miR-30e, miR-3196, miR-320, and let-7a-5p were not significantly different between these two groups (P > 0.05). Furthermore, plasma miR-150-5p (P = 0.016, r = -0.460) and miR-155-5p (P = 0.014, r = -0.467) were negatively correlated with the albuminuria excretion rate, while plasma miR-150-5p (P = 0.01, r = 0.318) and miR-155-5p (P = 0.030, r = 0.271) were positively correlated with the estimated glomerular filtration rate. Conclusion: miR-150-5p, miR-155-5p, miR-30e, miR-320e, and miR-3196 are potentially new diagnostic biomarkers for early DN. miR-150-5p and miR-155-5p may be involved in the pathogenesis and progression of DN. Further research is required to verify these findings and clarify the specific molecular mechanisms.


Assuntos
Diabetes Mellitus Tipo 2/sangue , Nefropatias Diabéticas/sangue , Perfilação da Expressão Gênica , MicroRNAs/sangue , Albuminúria/sangue , Biomarcadores/metabolismo , Líquidos Corporais/metabolismo , Estudos de Casos e Controles , Diabetes Mellitus Tipo 2/genética , Nefropatias Diabéticas/genética , Progressão da Doença , Feminino , Genótipo , Taxa de Filtração Glomerular , Humanos , Masculino , Pessoa de Meia-Idade , Análise de Sequência com Séries de Oligonucleotídeos , Curva ROC
16.
Sci Rep ; 9(1): 5871, 2019 04 10.
Artigo em Inglês | MEDLINE | ID: mdl-30971708

RESUMO

In order to develop an equation that integrates multiple clinical factors including signs and symptoms associated with uraemia to assess the initiation of dialysis, we conducted a retrospective cohort study including 25 haemodialysis centres in Mainland China. Patients with ESRD (n = 1281) who commenced haemodialysis from 2008 to 2011 were enrolled in the development cohort, whereas 504 patients who began haemodialysis between 2012 and 2013 were enrolled in the validation cohort comprised. An artificial neural network model was used to select variables, and a fuzzy neural network model was then constructed using factors affecting haemodialysis initiation as input variables and 3-year survival as the output variable. A logistic model was set up using the same variables. The equation's performance was compared with that of the logistic model and conventional eGFR-based assessment. The area under the bootstrap-corrected receiver-operating characteristic curve of the equation was 0.70, and that of two conventional eGFR-based assessments were 0.57 and 0.54. In conclusion, the new equation based on Fuzzy mathematics, covering laboratory and clinical variables, is more suitable for assessing the timing of dialysis initiation in a Chinese ESRD population than eGFR, and may be a helpful tool to quantitatively evaluate the initiation of haemodialysis.


Assuntos
Falência Renal Crônica/patologia , Redes Neurais de Computação , Adulto , Idoso , Área Sob a Curva , Feminino , Taxa de Filtração Glomerular , Humanos , Falência Renal Crônica/mortalidade , Falência Renal Crônica/terapia , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Curva ROC , Diálise Renal , Estudos Retrospectivos , Fatores de Tempo
17.
Conscious Cogn ; 69: 103-112, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30739068

RESUMO

Memories of past experiences can guide our decisions. Thus, if memories are undermined or distorted, decision making should be affected. Nevertheless, little empirical research has been done to examine the role of memory in reinforcement decision-making. We hypothesized that if memories guide choices in a conditioning decision-making task, then manipulating these memories would result in a change of decision preferences to gain reward. We manipulated participants' memories by providing false feedback that their memory associations were wrong before they made decisions that could lead them to win money. Participants' memory ratings decreased significantly after receiving false feedback. More importantly, we found that false feedback led participants' decision bias to disappear after their memory associations were undermined. Our results suggest that reinforcement decision-making can be altered by false feedback on memories. The results are discussed using memory mechanisms such as spreading activation theories.


Assuntos
Associação , Condicionamento Psicológico/fisiologia , Tomada de Decisões/fisiologia , Retroalimentação Psicológica/fisiologia , Rememoração Mental/fisiologia , Reforço Psicológico , Adolescente , Adulto , Feminino , Humanos , Masculino , Reconhecimento Visual de Modelos/fisiologia , Adulto Jovem
18.
J Invest Dermatol ; 139(2): 400-411, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30171832

RESUMO

The roles of IL-22 in the pathomechanisms of psoriasis have been well demonstrated. Gap junctional intercellular communication (GJIC) is widely known for its involvement in multiple biological and pathological processes such as growth-related events, cell differentiation, and inflammation. Here, we show that IL-22 significantly decreased GJIC and down-regulated Cx43 expression in HaCaT cells. Cx43 overexpression markedly inhibited the proliferation of and increased GJIC in HaCaT cells, but the silencing of Cx43 exerted the opposite effects. Additionally, Cx43 overexpression effectively rescued the IL-22-induced decrease in GJIC in HaCaT cells. The IL-22-induced down-regulation of Cx43 expression and decrease in GJIC can be significantly blocked by the JNK inhibitor SP600125 and by the overexpression of IL-22RA2 (which specifically binds to IL-22 and inhibits its activity), but not by the NF-κB inhibitor BAY11-7082, in HaCaT cells. Furthermore, the IL-22-induced down-regulation of Cx43 expression mediated by the JNK signaling pathway was confirmed in a mouse model of IL-22-induced psoriasis-like dermatitis. Similarly, Cx43 expression was significantly lower in the lesional skin than in the nonlesional skin of patients with psoriasis. These results suggest that IL-22 decreases GJIC by activating the JNK signaling pathway, which down-regulates Cx43 expression; this process is a possible pathomechanism of keratinocyte hyperproliferation in psoriasis.


Assuntos
Conexina 43/metabolismo , Interleucinas/metabolismo , Sistema de Sinalização das MAP Quinases/imunologia , Psoríase/patologia , Adulto , Antracenos/farmacologia , Comunicação Celular/efeitos dos fármacos , Comunicação Celular/imunologia , Linhagem Celular , Regulação para Baixo/efeitos dos fármacos , Feminino , Junções Comunicantes/efeitos dos fármacos , Junções Comunicantes/imunologia , Junções Comunicantes/patologia , Humanos , Interleucinas/imunologia , Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo , Queratinócitos , Masculino , Pessoa de Meia-Idade , Psoríase/imunologia , Pele/citologia , Pele/imunologia , Pele/patologia
19.
Mem Cognit ; 47(1): 76-86, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30141171

RESUMO

It is well established that processing information in relation to oneself (i.e., self-referencing) leads to better memory for that information than processing that same information in relation to others (i.e., other-referencing). However, it is unknown whether self-referencing also leads to more false memories than other-referencing does. In the current two experiments with European and East Asian samples, we presented participants the Deese-Roediger-McDermott lists together with their own name or other people's name (i.e., "Trump" in Experiment 1 and "Li Ming" in Experiment 2). We found consistent results across the two experiments; that is, in the self-reference condition, participants had higher true and false memory rates compared with those in the other-reference condition. Moreover, we found that self-referencing did not exhibit superior mnemonic advantage in terms of net accuracy compared with other-referencing and neutral conditions. These findings are discussed in terms of theoretical frameworks such as spreading activation theories and the fuzzy-trace theory. We propose that our results reflect the adaptive nature of memory in the sense that cognitive processes that increase mnemonic efficiency may also increase susceptibility to associative false memories.


Assuntos
Associação , Ego , Rememoração Mental/fisiologia , Reconhecimento Psicológico/fisiologia , Adolescente , Adulto , China , Feminino , Humanos , Masculino , Países Baixos , Teoria Psicológica , Adulto Jovem
20.
Braz. j. med. biol. res ; 52(1): e7952, 2019. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-974269

RESUMO

Malignant melanoma is an aggressive skin cancer with a high mortality rate. Nucleolar protein 14 (NOP14) has been implicated in cancer development. However, the role of NOP14 in malignant melanoma progression remains largely unclear. In this study, we observed that malignant melanoma tissue showed NOP14 down-regulation compared to melanocytic nevi tissues. Moreover, we observed that NOP14 expression was significantly associated with melanoma tumor thickness and lymph node metastasis. NOP14 overexpression in melanoma cells suppressed proliferation, caused G1 phase arrest, promoted apoptosis, and inhibited melanoma cell migration and invasion. Further investigations revealed that NOP14 overexpression reduced the expression levels of Wnt3a, β-catenin, and GSK-3β of the Wnt/β-catenin pathway. In summary, we demonstrated that NOP14 inhibited melanoma cell proliferation and metastasis by regulating the Wnt/β-catenin signaling pathway.

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