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1.
Nanoscale ; 11(36): 16708-16722, 2019 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-31469374

RESUMO

Self-assembly of colloids provides a powerful way for the construction of complex multi-scale materials. Microfluidic techniques possess great potential to precisely control the assembly of micro- and nano-scale building blocks via the rational design of various microfluidic environments. In this review, we first discuss the self-assembly of colloids without templates by using the laminar microfluidic technique. The self-assembly of colloids based on a droplet as a template was subsequently summarized and discussed via droplet microfluidic technique. Moreover, the evaporation-driven self-assembly of colloids in microfluidic channels has been discussed and analysed. Finally, the representative applications in this field have been pointed out. The aim of this review is to summarize the state-of-art on the self-assembly of colloids based on various microfluidic techniques, exhibit their representative applications, and point out the current challenges in this field, hoping to inspire and guide future work.

2.
Analyst ; 144(19): 5802-5809, 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31465037

RESUMO

A dual-mode sensing platform, involving fluorescence and reflectance modes, has been demonstrated for highly sensitive and selective detection of solvents and metal ions based on carbon dot-based inverse opal hydrogels (CD-IOHs). In this work, CD-IOHs have been first synthesized via the typical templating technique. Two kinds of CDs, including solvent and Cu(ii) ion sensitive CDs, have been incorporated into the matrix of IOHs during the co-polymerization of acrylic acid (AA) and 2-hydroxyethyl methacrylate (HEMA). The CD-IOHs not only appear green under daylight but also exhibit stable photoluminescence (PL) under UV light owing to the stop-band effect of photonic crystals and the quantum effect of CDs, respectively. By using these two optical phenomena, for solvent sensing, the CD-IOHs change their colors from green, yellow, and red to a semitransparent state and show good linear sensing with the ethanol content varying from 0 to 45% in reflectance mode, while their PL intensities exhibit a nonlinear detection trend: first an increase and then a decrease with the ethanol content in fluorescence mode. Remarkably, as for metal ion sensing, the CD-IOHs have high selectivity for Cu(ii) ions via the specific PL quenching effect of Cu(ii) ion sensitive CDs. Furthermore, the CD-IOHs show good linear detection in both modes and a wide linear detection range from 0.1 µM to 7 mM. Thus, high selectivity, colorimetric detection, a broad linear detection range, and dual-mode sensing can be realized using the CD-IOHs.

3.
Medicine (Baltimore) ; 98(27): e16199, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31277127

RESUMO

BACKGROUND: Clinical evidence indicates that women will benefit from regular physical activity during pregnancy. This study aimed to summarize and update the evidence on the effect of exercise on maternal gestational weight gain (GWG). METHODS: We conducted a systematic literature search of Pubmed, Embase, and Cochrane Library from inception until July, 2018 for randomized controlled trials (RCTs) that investigate the effect of physical exercises on the maternal GWG compared with that of no physical exercises or conventional medical care. We extracted data from eligible trials for study characteristics, interventions, patients' baseline characteristics and outcomes for the study populations of interest. We conducted meta-analyses using random effects models. RESULTS: From 844 citations, 23 RCTs including 4462 pregnant women met the inclusion criteria. Meta-analysis indicated that compared with that in women having conventional medical care, GWG was significantly decreased in pregnant women with physical exercise [weighted mean difference (WMD) -1.02, 95% CI -1.35 to -0.70; P < .01; I = 48.4%]. Women appeared to benefit more for gestational weight control for exercise frequency of 3 times per week (WMD -1.22, 95% CI -1.55 to -0.90; I = 40.3%) and exercise duration of 30 to 45 minutes each time (WMD -1.32, 95% CI -1.79 to -0.85; I = 1.5%). CONCLUSION: This meta-analysis provides indications that exercise intervention can reduce maternal GWG for pregnant women, especially for those with exercise frequency of 3 times per week and duration of 30 to 45 minutes each time.


Assuntos
Terapia por Exercício/métodos , Exercício/fisiologia , Ganho de Peso na Gestação/fisiologia , Sobrepeso/prevenção & controle , Complicações na Gravidez/prevenção & controle , Ensaios Clínicos Controlados Aleatórios como Assunto/métodos , Feminino , Humanos , Sobrepeso/fisiopatologia , Gravidez , Complicações na Gravidez/fisiopatologia
4.
Cell Mol Gastroenterol Hepatol ; 8(3): 475-486, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31195150

RESUMO

BACKGROUND & AIMS: The mammalian intestinal epithelium is a rapidly self-renewing tissue in the body, and its homeostasis is tightly regulated via well-controlled mechanisms. The RNA-binding protein HuR is essential for maintaining gut epithelial integrity, and targeted deletion of HuR in intestinal epithelial cells (IECs) disrupts mucosal regeneration and delays repair after injury. Here, we defined the role of HuR in regulating subcellular distribution of small guanosine triphosphatase Rac1 and investigated the implication of nucleophosmin (NPM) as a molecular chaperone in this process. METHODS: Studies were conducted in intestinal epithelial tissue-specific HuR knockout (IE-HuR-/-) mice and cultured IEC-6 cells, derived from rat small intestinal crypts. Functions of HuR and NPM in vitro were investigated via their gene silencing and overexpression. RESULTS: The abundance of cytoplasmic Rac1 in the small intestinal mucosa increased significantly in IE-HuR-/- mice, although HuR deletion did not alter total Rac1 levels. HuR silencing in cultured IECs also increased the cytoplasmic Rac1 levels, without an effect on whole-cell Rac1 content. In addition, HuR deficiency in the intestinal epithelium decreased the levels of NPM in IE-HuR-/- mice and cultured IECs. NPM physically interacted with Rac1 and formed the NPM/Rac1 complex. NPM silencing decreased the NPM/Rac1 association and inhibited nuclear accumulation of Rac1, along with an increase in cytoplasmic abundances of Rac1. In contrast, ectopically expressed NPM enhanced Rac1 nuclear translocation and restored Rac1 subcellular localization to near normal in HuR-deficient cells. CONCLUSIONS: These results indicate that HuR regulates Rac1 nucleocytoplasmic shuttling in the intestinal epithelium by altering NPM expression.

5.
Gastroenterology ; 157(3): 731-743, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31103627

RESUMO

BACKGROUND & AIMS: Paneth cells secrete antimicrobial proteins including lysozyme via secretory autophagy as part of the mucosal protective response. The ELAV like RNA-binding protein 1 (ELAVL1, also called HuR) regulates stability and translation of messenger RNAs (mRNAs) and many aspects of mucosal physiology. We studied the posttranscriptional mechanisms by which HuR regulates Paneth cell function. METHODS: Intestinal mucosal tissues were collected from mice with intestinal epithelium (IE)-specific disruption of HuR (IE-HuR-/-), HuRfl/fl-Cre- mice (controls), and patients with inflammatory bowel diseases and analyzed by histology and immunohistochemistry. Paneth cell functions were determined by lysozyme-immunostaining assays. We isolated primary enterocytes from IE-HuR-/- and control mice and derived intestinal organoids. HuR and the chaperone CNPY3 were overexpressed from transgenes in intestinal epithelial cells (IECs) or knocked down with small interfering RNAs. We performed RNA pulldown assays to investigate interactions between HuR and its target mRNAs. RESULTS: Intestinal tissues from IE-HuR-/- mice had reduced numbers of Paneth cells, and Paneth cells had fewer lysozyme granules per cell, compared with tissues from control mice, but there were no effects on Goblet cells or enterocytes. Intestinal mucosa from patients with inflammatory bowel diseases had reduced levels of HuR and fewer Paneth cells. IE-HuR-/- mice did not have the apical distribution of TLR2 in the intestinal mucosa as observed in control mice. IECs from IE-HuR-/- mice expressed lower levels of CNPY3. Intestinal organoids from IE-HuR-/- mice were smaller and contained fewer buds compared with those generated from controls, and had fewer lysozyme-positive cells. In IECs, knockdown of HuR decreased levels of the autophagy proteins LC3-I and LC3-II, compared with control cells, and prevented rapamycin-induced autophagy. We found HuR to interact directly with the Cnpy3 mRNA coding region and increase levels of CNPY3 by increasing the stability and translation of Cnpy3 mRNA. CNPY3 bound TLR2, and cells with knockdown of CNPY3 or HuR lost membrane localization of TLR2, but increased cytoplasmic levels of TLR2. CONCLUSIONS: In studies of mice, IECs, and human tissues, we found HuR to increase expression of CNPY3 at the posttranscriptional level. CNPY3 is required for membrane localization of TLR2 and Paneth cell function.


Assuntos
Membrana Celular/metabolismo , Proteína Semelhante a ELAV 1/metabolismo , Intestino Delgado/metabolismo , Chaperonas Moleculares/metabolismo , Celulas de Paneth/metabolismo , Processamento Pós-Transcricional do RNA , Receptor 2 Toll-Like/metabolismo , Animais , Estudos de Casos e Controles , Células Cultivadas , Proteína Semelhante a ELAV 1/deficiência , Proteína Semelhante a ELAV 1/genética , Humanos , Doenças Inflamatórias Intestinais/genética , Doenças Inflamatórias Intestinais/metabolismo , Doenças Inflamatórias Intestinais/patologia , Intestino Delgado/patologia , Camundongos Endogâmicos C57BL , Camundongos Knockout , Chaperonas Moleculares/genética , Celulas de Paneth/patologia , Transporte Proteico , Transdução de Sinais , Regulação para Cima
6.
Biosens Bioelectron ; 136: 91-96, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31039492

RESUMO

Highly sensitive and low-cost DNA detection are in great desire owing to the significant role of DNA molecules in detecting genetic damage and errors for the diagnosis and treatment of multiple diseases. Traditional detections of DNA mainly rely on large-scale instrument, which requires the complicated detection process and high cost. Solution-gated electrochemical transistors are widely studied due to high sensitivity and low cost. Here, we exploit a graphene electrochemical transistor for the efficient and sensitive DNA detection. The probe DNA is modified on the gate electrode to detect the target DNA molecules. A novel method is introduced to modify the gate electrode with the probe DNA to detect the different concentration of target DNA. Herein, in comparison with previous reported methods, our DNA sensors show a good limit of detection in the range of 1 fM - 5 µM. The highly sensitive and selective validate the developed DNA sensor as a promising tool for routine use.


Assuntos
Técnicas Biossensoriais/métodos , DNA/análise , Transistores Eletrônicos , Eletrodos , Ouro/química , Grafite/química
7.
BMC Vet Res ; 15(1): 160, 2019 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-31118049

RESUMO

BACKGROUND: African swine fever (ASF) is a devastating infectious disease of pigs. ASF poses a potential threat to the world pig industry, due to the lack of vaccines and treatments. In this study, the Geographic Information System (GIS) spatial analysis was applied to analyze the distribution, dispersion of the epidemic and clustering of ASF in Poland. RESULTS: The results show that the center of the epidemic moved gradually towards the southwest, and the distribution of the epidemic changed from south-north to east-west. Through space-time scan statistical analysis, the 3 clusters major of wild boar cases involve longer time spans and larger radii, while the other five with higher relative risks involved in domestic pigs. And then, a quantitative model was constructed to analyse the risk of releasing African swine fever virus (ASFV) from Poland by the legal export of pork and pork products. The Latin hypercube sampling results show that the probability is relatively low (the average value is 4.577 × 10- 7). CONCLUSIONS: All the identification of the spatio-temporal patterns of the epidemic and the risk analysis model would give a further understanding of the dynamics of disease transmission and help to design corresponding measures to minimize the catastrophic consequences of potential ASFV introduction.


Assuntos
Febre Suína Africana/epidemiologia , Modelos Estatísticos , Febre Suína Africana/transmissão , Animais , Polônia , Medição de Risco , Análise Espaço-Temporal , Suínos
8.
Am J Physiol Cell Physiol ; 317(1): C93-C100, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31042423

RESUMO

The epithelium of the mammalian intestinal mucosa is a rapidly self-renewing tissue in the body, and its homeostasis is preserved through well-controlled mechanisms. Long noncoding RNAs (lncRNAs) regulate a variety of biological functions and are intimately involved in the pathogenesis of diverse human diseases. Here we highlight the roles of several lncRNAs expressed in the intestinal epithelium, including uc.173, SPRY4-IT1, H19, and Gata6, in maintaining the integrity of the intestinal epithelium, focusing on the emerging evidence of lncRNAs in the regulation of intestinal mucosal regeneration and epithelial barrier function. We also discuss recent results that the interactions between lncRNAs with microRNAs and the RNA-binding protein HuR influence epithelial homeostasis. With rapidly advancing knowledge of lncRNAs, there is also growing recognition that lncRNAs in the intestinal epithelium might be promising therapeutic targets in our efforts to protect the integrity of the intestinal epithelium in response to stressful environments.

9.
Genome Biol ; 20(1): 103, 2019 05 24.
Artigo em Inglês | MEDLINE | ID: mdl-31126313

RESUMO

BACKGROUND: Inherited factors contribute to lung cancer risk, but the mechanism is not well understood. Defining the biological consequence of GWAS hits in cancers is a promising strategy to elucidate the inherited mechanisms of cancers. The tag-SNP rs753955 (A>G) in 13q12.12 is highly associated with lung cancer risk in the Chinese population. Here, we systematically investigate the biological significance and the underlying mechanism behind 13q12.12 risk locus in vitro and in vivo. RESULTS: We characterize a novel p53-responsive enhancer with lung tissue cell specificity in a 49-kb high linkage disequilibrium block of rs753955. This enhancer harbors 3 highly linked common inherited variations (rs17336602, rs4770489, and rs34354770) and six p53 binding sequences either close to or located between the variations. The enhancer effectively protects normal lung cell lines against pulmonary carcinogen NNK-induced DNA damages and malignant transformation by upregulating TNFRSF19 through chromatin looping. These variations significantly weaken the enhancer activity by affecting its p53 response, especially when cells are exposed to NNK. The effect of the mutant enhancer alleles on TNFRSF19 target gene in vivo is supported by expression quantitative trait loci analysis of 117 Chinese NSCLC samples and GTEx data. Differentiated expression of TNFRSF19 and its statistical significant correlation with tumor TNM staging and patient survival indicate a suppressor role of TNFRSF19 in lung cancer. CONCLUSION: This study provides evidence of how the inherited variations in 13q12.12 contribute to lung cancer risk, highlighting the protective roles of the p53-responsive enhancer-mediated TNFRSF19 activation in lung cells under carcinogen stress.


Assuntos
Cromossomos Humanos Par 13 , Elementos Facilitadores Genéticos , Neoplasias Pulmonares/genética , Receptores do Fator de Necrose Tumoral/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Apoptose , Linhagem Celular Tumoral , Reparo do DNA , Regulação da Expressão Gênica , Predisposição Genética para Doença , Células HEK293 , Humanos , Desequilíbrio de Ligação , Neoplasias Pulmonares/metabolismo , Polimorfismo de Nucleotídeo Único
10.
J Asian Nat Prod Res ; : 1-12, 2019 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-30963782

RESUMO

Two new xanthones smilone A (1), smilone B (2), and a new lignin smilgnin A (3) were isolated from the rhizomes of Smilax china L., together with three known xanthones (4-6), four lignins (7-10), two flavones (11, 12), two stilbenoids (13, 14), and ten organic phenoloids (15-24). Of them, compounds 4-6 were isolated from the genus Smilax for the first time. The structures of 1-24 were elucidated by the extensive analysis of spectral data and compared with the literature. All compounds were evaluated for their inhibitory effects against LPS-induced NO production in RAW264.7 macrophages. Among them, compound 24 exhibited significant inhibitory activity against NO production (IC50 = 1.26 µM), while compounds 3, 6, and 7 showed weak activities at the concentration of 50 µM.

11.
Curr Mol Med ; 19(5): 349-356, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30950353

RESUMO

BACKGROUND: SOX15 is a crucial transcription factor involved in the regulation of embryonic development and in the cell fate determination. It is also an important mediator of tumorigenesis in cancer. METHODS: Here, we sought to explore the expression patterns and biological functions of SOX15 in esophageal squamous cell carcinomas (ESCC). SOX15 was found aberrantly overexpressed in ESCC tumors. RESULTS: Experimentally, inhibition of SOX15 through RNAi suppressed cell proliferation in ESCC cells and sensitized cancer cells to paclitaxel, but not to Cisplatin. Moreover, inhibition of SOX15 significantly repressed the expression of genes associated with WNT and NOTCH signaling pathways, which may contribute to the increased sensitivity to paclitaxel. CONCLUSION: In conclusion, the current study revealed that inhibition of SOX15 in ESCC cells sensitizes the ESCC cells to paclitaxel, suggesting that the SOX15 expression level may predict the therapeutic outcomes for paclitaxel treatment for ESCC.

12.
Zhongguo Zhong Yao Za Zhi ; 44(1): 28-33, 2019 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-30868808

RESUMO

Temporary prescription preparation is the preparation processed into different dosage forms by relevant pharmacist according to the temporary preparation requirement and the personalized prescription made by the doctor in accordance with the syndrome differentiation and drug performance.It is an important part in personalized pharmaceutical services.Rational design of process route,production equipment and quality control method for the temporary prescription preparation,and establishment of technology research strategy and mode in accordance with the characteristics of traditional Chinese medicine temporary prescription preparations play an important role in promoting the development of the temporary prescription preparations.To promote the normalization,standardization and intelligent development of temporary prescription preparations,we would comprehensively summarize the significance,policy,technology characteristics,technology research status quo and existing problems in this paper,and put forward the research direction of temporary prescription preparation technology based on the physical properties of raw materials,equipment research strategy,and intelligent manufacturing technology.Thus it will push the inheritance and innovation of temporary prescription preparation.


Assuntos
Medicina Tradicional Chinesa , Prescrições/normas , Projetos de Pesquisa , Controle de Qualidade
13.
Nanoscale ; 11(13): 6401-6409, 2019 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-30888390

RESUMO

In this work, we have adopted a facile three-step method for constructing an intriguing bifunctional electrode of self-supported hollow (Ni,Co)Se2 arrays with a metal-organic framework (MOF) precursor. The triangle-like cobalt-based MOF arrays are first grown on a carbon cloth at room temperature, which is followed by an ion exchange/etching process with Ni(NO3)2 to form a critical hollow nanostructure with an incorporated hetero-metal element. The intermediate is then transformed into the final product through solvothermal selenization treatment. Taking advantages of the structural and compositional merits as well as the self-supporting nature, the resultant (Ni,Co)Se2 electrode exhibits excellent electrochemical activity and stability. When tested as an electrocatalyst for the oxygen evolution reaction (OER), the (Ni,Co)Se2 array electrode displayed a low onset overpotential of 226 mV and a small overpotential of 256 mV to afford a current density of 10 mA cm-2. The (Ni,Co)Se2 electrode is also utilized in a supercapacitor, which delivers a high specific capacitance of 2.85 F cm-2 at 2 mA cm-2 and exhibits excellent cycling stability with a capacitance retention of 80.8% after 2000 charge-discharge cycles at 20 mA cm-2. These results demonstrate the significance of the rational design of electrode materials and disclose the potential of our MOF-derived hollow (Ni,Co)Se2 array electrode for a variety of practical applications in energy conversion and storage.

14.
Nature ; 568(7751): E3, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30911170

RESUMO

In this Letter, a citation to 'Fig. 1e' has been corrected to 'Fig. 1d' in the sentence starting "By contrast, the anti-tumour response…". This has been corrected online.

15.
Nature ; 566(7743): 270-274, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30728504

RESUMO

There is growing evidence that tumour neoantigens have important roles in generating spontaneous antitumour immune responses and predicting clinical responses to immunotherapies1,2. Despite the presence of numerous neoantigens in patients, complete tumour elimination is rare, owing to failures in mounting a sufficient and lasting antitumour immune response3,4. Here we show that durable neoantigen-specific immunity is regulated by mRNA N6-methyadenosine (m6A) methylation through the m6A-binding protein YTHDF15. In contrast to wild-type mice, Ythdf1-deficient mice show an elevated antigen-specific CD8+ T cell antitumour response. Loss of YTHDF1 in classical dendritic cells enhanced the cross-presentation of tumour antigens and the cross-priming of CD8+ T cells in vivo. Mechanistically, transcripts encoding lysosomal proteases are marked by m6A and recognized by YTHDF1. Binding of YTHDF1 to these transcripts increases the translation of lysosomal cathepsins in dendritic cells, and inhibition of cathepsins markedly enhances cross-presentation of wild-type dendritic cells. Furthermore, the therapeutic efficacy of PD-L1 checkpoint blockade is enhanced in Ythdf1-/- mice, implicating YTHDF1 as a potential therapeutic target in anticancer immunotherapy.


Assuntos
Adenosina/análogos & derivados , Adenosina/metabolismo , Células Dendríticas/imunologia , Neoplasias/imunologia , RNA Mensageiro/metabolismo , Proteínas de Ligação a RNA/metabolismo , Animais , Apresentação do Antígeno/imunologia , Antígenos de Neoplasias/imunologia , Antígenos de Neoplasias/metabolismo , Antígeno B7-H1/metabolismo , Sítios de Ligação , Linfócitos T CD8-Positivos/imunologia , Catepsinas/antagonistas & inibidores , Catepsinas/biossíntese , Catepsinas/genética , Apresentação Cruzada/imunologia , Células Dendríticas/enzimologia , Feminino , Humanos , Metilação , Camundongos , Camundongos Endogâmicos C57BL , Neoplasias/terapia , Biossíntese de Proteínas , Proteínas/genética , RNA Mensageiro/química , Proteínas de Ligação a RNA/genética , Transcriptoma/genética
16.
Chem Rec ; 19(10): 2083-2094, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30762933

RESUMO

Carbon dots (CDs), a new class of fluorescent carbon nanoparticles (less than 10 nm in size), have been widely applied in various fields, including sensors, bioimaging, catalysis, light-emitting devices (LEDs), and photoelectronic devices, owing to their unique properties such as low toxicity, bio-compatibility, high photostability, easy surface modification, and up-conversion fluorescence, over the past decades. Recently, multiple-color-emissive CDs, especially red-emissive CDs (RCDs), have drawn much attention owing to their unique advantages, like the ability to penetrate the animal bodies without the disturbance of strong tissue autofluorescence, multiple-color fluorescence displaying or sensing, and the capacity to be one essential component to obtain white LED (WLED). In this review, we focused on the progress of recently-emerging RCDs in the past five years, including their synthetic methods (hydrothermal, solvothermal, reflux condensation and microwave techniques), influencing factors (precursors, solvents, elements doping, surface chemistry) and various applications (bioimaging, sensor, photocatalysis and WLEDs), with a perspective on the future advancements.

17.
Am J Physiol Cell Physiol ; 316(3): C415-C423, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30649922

RESUMO

Both zipcode binding protein-1 (ZBP1) and phospholipase C-γ1 (PLCγ1) are intimately involved in many aspects of early intestinal mucosal repair after acute injury, but the exact mechanisms that control their cellular abundances remain largely unknown. The present study shows that microRNA-222 (miR-222) interacts with the mRNAs encoding ZBP1 and PLCγ1 and regulates ZBP1 and PLCγ1 expression in intestinal epithelial cells (IECs). The biotinylated miR-222 bound specifically to the ZBP1 and PLCγ1 mRNAs in IECs. Ectopically expressed miR-222 precursor destabilized the ZBP1 and PLCγ1 mRNAs and consequently lowered the levels of cellular ZBP1 and PLCγ1 proteins. Conversely, decreasing the levels of cellular miR-222 by transfection with its antagonism increased the stability of the ZBP1 and PLCγ1 mRNAs and increased the levels of ZBP1 and PLCγ1 proteins. Overexpression of miR-222 also inhibited cell migration over the wounded area, which was partially abolished by overexpressing ZBP1 and PLCγ1. Furthermore, prevention of the increased levels of ZBP1 and PLCγ1 in the miR-222-silenced cells by transfection with specific small interfering RNAs targeting ZBP1 or PLCγ1 mRNA inhibited cell migration after wounding. These findings indicate that induced miR-222 represses expression of ZBP1 and PLCγ1 at the posttranscriptional level, thus inhibiting IEC migration during intestinal epithelial restitution after wounding.

18.
Chin Med J (Engl) ; 132(3): 319-328, 2019 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-30681498

RESUMO

BACKGROUND: Eucommia ulmoides Oliv. is a medicinal plant native to China, with its bark (Eucommiae Cortex) traditionally being used for medicinal purposes. Previous research has shown that Eucommia male flowers can exert anti-inflammatory, analgesic, antibacterial, and other pharmacological effects, including immune regulation. This study explored the anti-inflammatory effects of the 70% ethanol extract of male flowers (EF) of E. ulmoides in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and LPS-administered mice. METHODS: Cytotoxicity of EF for RAW 264.7 cells was investigated using Cell Counting Kit-8. The production of proinflammatory mediators, nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1ß, and IL-6 was determined using enzyme-linked immunosorbent assays. IL-17, IL-23, and IL-10 mRNA levels were determined using quantitative real-time polymerase chain reaction. Activation of the nuclear factor (NF)-κB pathway in RAW 264.7 cells was investigated via Western blotting. In vivo anti-inflammatory effects of EF were studied in an LPS-induced acute inflammation mouse model by analyzing lung tissue histopathology, serum TNF-α and IL-6 levels, and myeloperoxidase (MPO) activity in lung tissue. RESULTS: EF showed no significant cytotoxicity at concentrations from 10 to 60 µg/mL (cell viability > 80%) in the CCK-8 cell viability assay. EF inhibited the RAW 264.7 cell proliferation (EF 60 µg/mL, 120 µg/mL, and 250 µg/mL vs. negative control: 87.31 ±â€Š2.39% vs. 100.00 ±â€Š2.50%, P = 0.001; 79.01 ±â€Š2.56 vs. 100.00 ±â€Š2.50%, P < 0.001; and 64.83 ±â€Š2.50 vs. 100.00 ±â€Š2.50%, P < 0.001), suppressed NO (EF 20 µg/mL and 30 µg/mL vs. LPS only, 288.81 ±â€Š38.01 vs. 447.68 ±â€Š19.07 µmol/L, P = 0.004; and 158.80 ±â€Š45.14 vs. 447.68 ±â€Š19.07 µmol/L, P < 0.001), TNF-α (LPS+EF vs. LPS only, 210.20 ±â€Š13.85 vs. 577.70 ±â€Š5.35 pg/mL, P < 0.001), IL-1ß (LPS+EF vs. LPS only, 193.30 ±â€Š10.80 vs. 411.03 ±â€Š42.28 pg/mL, P < 0.001), and IL-6 (LPS+EF vs. LPS only, 149.67 ±â€Š11.60 vs. 524.80 ±â€Š6.24 pg/mL, P < 0.001) secretion, and downregulated the mRNA expression of IL-17 (LPS+EF vs. LPS only, 0.23 ±â€Š0.02 vs. 0.43 ±â€Š0.12, P < 0.001), IL-23 (LPS+EF vs. LPS only, 0.29 ±â€Š0.01 vs. 0.42 ±â€Š0.06, P=0.002), and IL-10 (LPS+EF vs. LPS only, 0.30 ±â€Š0.01 vs. 0.47 ±â€Š0.01, P=0.008) in LPS-stimulated RAW 264.7 cells. EF inhibited the LPS-induced NF-κB p65 (LPS+EF 20 µg/mL and 30 µg/mL vs. LPS only: 0.78 ±â€Š0.06 vs. 1.17 ±â€Š0.08, P < 0.001; and 0.90 ±â€Š0.06 vs. 1.17 ±â€Š0.08, P =0.002) and inhibitor of kappa B (IκBα) phosphorylation (LPS+EF 20 µg/mL and 30 µg/mL vs. LPS only: 0.25 ±â€Š0.01 vs. 0.63 ±â€Š0.03, P < 0.001; and 0.31 ±â€Š0.01 vs. 0.63 ±â€Š0.03, P < 0.001), LPS+EF 30 µg/mL inhibited IκB kinase (IKKα/ß) phosphorylation (LPS+EF 30 µg/mL vs. LPS only, 1.12 ±â€Š0.14 vs. 1.71 ±â€Š0.25, P = 0.002) in RAW 264.7 cells. Furthermore, EF 10 mg/kg and EF 20 mg/kg inhibited lung tissue inflammation in vivo and suppressed the serum TNF-α (LPS+EF 10 mg/kg and 20 mg/kg vs. LPS only, 199.99 ±â€Š186.49 vs. 527.90 ±â€Š263.93 pg/mL, P=0.001; and 260.56 ±â€Š175.83 vs. 527.90 ±â€Š263.93 pg/mL, P = 0.005), and IL-6 (LPS+EF 10 mg/kg and 20 mg/kg vs. LPS only, 41.26 ±â€Š30.42 vs. 79.45 ±â€Š14.16 pg/ ml, P = 0.011; and 42.01 ±â€Š26.26 vs. 79.45 ±â€Š14.16 pg/mL, P = 0.012) levels and MPO (LPS+EF 10 mg/kg and 20 mg/kg vs. LPS only, 3.19 ±â€Š1.78 vs. 5.39 ±â€Š1.51 U/g, P = 0.004; and 3.32 ±â€Š1.57 vs. 5.39 ±â€Š1.51 U/g, P = 0.006) activity in lung tissue. CONCLUSIONS: EF could effectively inhibit the expression of inflammatory factors and overactivation of neutrophils. Further investigation is needed to evaluate its potential for anti-inflammation therapy.


Assuntos
Anti-Inflamatórios/uso terapêutico , Eucommiaceae/química , Flores/química , Inflamação/induzido quimicamente , Inflamação/tratamento farmacológico , Lipopolissacarídeos/toxicidade , Extratos Vegetais/química , Extratos Vegetais/uso terapêutico , Animais , Anti-Inflamatórios/química , Inflamação/sangue , Interleucina-1beta/sangue , Macrófagos/efeitos dos fármacos , Camundongos , Inibidor de NF-kappaB alfa/sangue , NF-kappa B/sangue , Óxido Nítrico/sangue , Células RAW 264.7 , Transdução de Sinais/efeitos dos fármacos , Fator de Necrose Tumoral alfa/sangue
19.
Iran J Public Health ; 47(10): 1546-1551, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30524985

RESUMO

Background: To investigate the effect of applying doctor-nurse-patient integration management mode to patients with endometriosis (EMT). Methods: A total of 160 patients with EMT from the Department of Neurology of The Fifth Affiliated Hospital Sun Yat-Sen University from January 2017 to October 2017 were selected. They were divided into control group and experimental group according to the time sequence of hospitalization, with 80 patients in each group. The traditional nursing management mode was implemented in the control group, and doctor-nurse-patient integration mode was implemented in the experimental group. The psychological status, quality of life, and satisfaction of the 2 groups of patients were compared one year after surgery. Results: The anxiety and depression scores in the observation group were (41.89±7.50) and (42.40±7.40) points, respectively, and those in the control group were (57.55±9.68) and (55.00±9.35) points, respectively. The differences between the two groups were statistically significant (t=-11.44, -9.42, P<0.05). The improvement rate of quality of life (sleep, work and sexual life) in the observation group was 87.5%, which was higher than that in the control group (63.8%). The difference between the 2 groups was statistically significant (U=583, P<0.01). The satisfaction rate in the observation group was 90.00%, which was higher than that in the control group (78.75%) (U=592.00, P< 0.01). Conclusion: The doctor-nurse-patient integration management mode can effectively improve the negative psychological status and quality of life of patients with EMT and improve patient satisfaction, which is worth popularizing.

20.
Lipids Health Dis ; 17(1): 289, 2018 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-30572889

RESUMO

BACKGROUND: The present study evaluated the predictive ability of five known "best" obesity and lipid-related parameters, including body mass index (BMI), waist-to-height ratio (WHtR), triglyceride-to-high-density-lipoprotein-cholesterol (TG/HDL-C), lipid accumulation product (LAP) and visceral adiposity index (VAI), in identifying metabolic syndrome (MetS) in Chinese elderly population. METHODS: A total of 6722 elderly Chinese subjects (≥60 years) were recruited into our community-based cross-sectional study from April 2015 to July 2017. The anthropometrics, blood pressure, fasting plasma glucose, blood lipid profiles, family history and health-related behaviours were assessed. RESULTS: The prevalence of MetS was 40.4% (32.5% in males and 47.2% in females). With the increase in the number of MetS components (from 0 to 5), all the five parameters showed an increase trend in both genders (all P for trend < 0.001). According to receiver operating characteristic curve (ROC) analyses, all the five parameters performed high predictive value in identifying MetS. The statistical significance of the areas under the curves (AUCs) differences suggested that the AUCs of LAP were the greatest among others in both genders (AUCs were 0.897 in males and 0.875 in females). The optimal cut-off values of LAP were 26.35 in males and 31.04 in females. After adjustment for potentially confounding factors, LAP was strongly associated with the odds of having MetS in both genders, and ORs for MetS increased across quartiles using multivariate logistic regression analysis (P < 0.001). CONCLUSION: LAP appeared to be a superior parameter for predicting MetS in both Chinese elderly males and females, better than VAI, TG/HDL-C, WHtR and BMI.


Assuntos
Síndrome Metabólica/sangue , Síndrome Metabólica/diagnóstico , Idoso , Área Sob a Curva , Índice de Massa Corporal , Estudos Transversais , Feminino , Humanos , Produto da Acumulação Lipídica , Lipoproteínas HDL , Masculino , Pessoa de Meia-Idade , Prognóstico , Curva ROC , Triglicerídeos
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