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1.
Opt Lett ; 46(19): 4976-4979, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34598258

RESUMO

This study proposes a polychromatic interferometric illumination and polarimetric sensor-based imaging method for spectrum, polarization, and 3D shape, which are significant physical parameters of feature analysis for target detection. 5D-fusion sensing refers to the joint detection and fusion of the above 5D information, which is currently a great challenge. The method generates a polychromatic interference pattern using a Sagnac lateral shearing interferometer and projects it to the target. Then, interferograms modulated by the target are acquired during scanning. Fast Fourier transform (FFT) is performed on the interferograms to obtain their frequency spectra. The spectral and polarization information is extracted from the moduli of the frequency spectra. The 3D shape is recovered from the phase of the frequency spectra using the calibration data. The theory of 5D-fusion sensing is investigated, and verification experiments are then performed. The experiments indicate that the proposed method can fulfill 5D-fusion sensing in one scanning and with FFT using only one device compared with other separate methods. Consequently, the proposed method can improve the sensing and recognition ability of optical imaging technology, which provides great application potential in biomedicine, food safety, material analysis, criminal investigation, archeology, and other fields.

2.
Environ Pollut ; 292(Pt A): 118374, 2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34656684

RESUMO

Ni(II) and chelated Ni(II) in wastewater are of environmental concern. This study explores the sequestration potential of structural Fe(II) in solid phase (≡Fe(II)) on Ni(II) and EDTA-Ni(II) using freshly prepared ferrous hydroxyl complex (FHC) as the Fe(II)-bearing mineral. The 1 mM Ni(II) could be completely sequestrated in 20 min by 3 mM FHC, although the sequestrated Ni(II) was partially released after 20 min. It is calculated that up to 156 mg Ni(II)/g Fe(II) can be sequestrated by ≡Fe(II) under neutral pH and anaerobic condition. According to the characterizations of the solid products, the large surface area for Ni(II) adsorption and the high ≡Fe(II) reduction capacity for Ni(II) reduction are the main contributors to the Ni(II) sequestration. After the reaction, the FHC is transformed to stable Fe-Ni layered double hydroxides. The concomitant ions can be either promotional or inhibitory to the sequestration performance depending on the ion type. The combination of FHC and Fe(III) can effectively sequestrate EDTA-Ni(II), whereas FHC alone has a low efficiency. Fe(III) substitutes Ni(II) from the EDTA-Ni(II), benefiting the subsequent Ni(II) sequestration by ≡Fe(II). This study demonstrates that ≡Fe(II) suspension is an cost-effective option for remediating Ni(II)-containing wastewater.

3.
J Biomed Nanotechnol ; 17(8): 1486-1509, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-34544528

RESUMO

Immunotherapy displays potent potential for clinical cancer management by activating the protective immune response; however, the microenvironment of the immunosuppressive tumor restricts the efficiency of immunotherapies. Along with the complex pathophysiological barrier of the solid tumors, successful immunotherapeutic delivery remains a formidable challenge for conventional nanomedicine. Stimuli-sheddable nano vectors may facilitate the delivery of cargoes to tumors with minimal premature cargo leakage in blood circulation while enhancing the tumor penetration of nanomedicines by deshielding the polyethylene glycol (PEG) corona upon endogenous activity such as acidity, enzymes and glutathione, or external stimuli, such as laser irradiation. Throughout this study, researchers overviewed the recent advances of nanomedicine-based cancer immunotherapy using the stimuli-responsive deshielding nano vectors, which allowed researchers to integrate multiple therapeutic regimens for inducing immunogenic cell death. This aided in blocking the immune checkpoints, repolarizing the macrophages, and regulating the kynurenine metabolism. Furthermore, researchers discussed the critical issues in the development of stimuli-sheddable nanoimmunodulators, primarily aimed at speeding up their clinical translation. Finally, researchers provided novel perspectives for improving cancer management with the stimuli-sheddable nanomedicine.


Assuntos
Nanomedicina , Neoplasias , Humanos , Morte Celular Imunogênica , Imunoterapia , Neoplasias/terapia , Polietilenoglicóis , Microambiente Tumoral
4.
Front Neurosci ; 15: 685685, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34489622

RESUMO

Background: Vagus nerve stimulation (VNS) has been demonstrated to be safe and effective for patients with refractory epilepsy, but there are few reports on the use of VNS for postencephalitic epilepsy (PEE). This retrospective study aimed to evaluate the efficacy of VNS for refractory PEE. Methods: We retrospectively studied 20 patients with refractory PEE who underwent VNS between August 2017 and October 2019 in Chinese PLA General Hospital and Beijing Children's Hospital. VNS efficacy was evaluated based on seizure reduction, effective rate (percentage of cases with seizure reduction ≥ 50%), McHugh classification, modified Early Childhood Epilepsy Severity Scale (E-Chess) score, and Grand Total EEG (GTE) score. The follow-up time points were 3, 6, and 12 months after VNS. Pre- and postoperative data were compared and analyzed. Results: The median [interquartile range (IQR)] seizure reduction rates at 3, 6, and 12 months after VNS were 23.72% (0, 55%), 46.61% (0, 79.04%), and 67.99% (0, 93.78%), respectively. The effective rates were 30% at 3 months, 45% at 6 months, and 70% at 12 months. E-chess scores before the operation and at 3, 6, and 12 months after the operation were 10 (10, 10.75), 9 (9, 10), 9 (9, 9.75), and 9 (8.25, 9) (P < 0.05), respectively. GTE scores before surgery and at 12 months after the operation were 11 (9, 13) and 9 (7, 11) (P < 0.05), respectively. The mean intensity of VNS current was 1.76 ± 0.39 (range: 1.0-2.5) mA. No intraoperative complications or severe post-operative adverse effects were reported. Conclusions: Our study shows that VNS can reduce the frequency and severity of seizure in patients with refractory PEE. VNS has a good application prospect in patients with refractory PEE.

5.
Mitochondrial DNA B Resour ; 6(10): 2910-2912, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34553045

RESUMO

Hypomecis punctinalis Scopoli, 1763 belongs to the Lepidopteran family Geometridae. We sequenced the complete mitochondrial genome (mitogenome) of H. punctinalis. The mitogenome is 15,648 bp long and contains a typical set of genes (13 protein-coding genes (PCGs), 22 tRNA genes, and two rRNA genes) and a 484 bp AT-rich region. All PCGs start with ATN codons and stop at TAA codon except for cox1 using CGA as initiation codon and nad4 and nad5 using incomplete termination codon T. Within the mitogenome, 17 intergenic spacers and seven overlaps are founded. The intergenic nucleotides are 294 bp in total and two longest intervals locate between trnGln and nad2 as well as trnCys and trnTyr . The overlap nucleotides are 47 bp in total and the maximum overlap lies between cox2 and trnLys . The AT-rich region of the mitogenome contains an 'ATAGA + polyT' motif, three copies of 30-bp-repeat and a short polyA tail. The phylogenetic tree shows the relationships of four subfamilies of Geometridae are (((Ennominae + Geometrinae)+Larentiinae)+Sterrhinae)) and the relationships within subfamily Ennominae are ((((Erannis+Biston)+(Jankowskia+(Hypomecis+(Apocheima+Milionia))))+Ectropis)+Abraxas)+Phthonandria)+Celenna).

6.
Chem Commun (Camb) ; 57(78): 10051-10054, 2021 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-34505863

RESUMO

We herein realize the first example of using a microporous HOF material (ZJU-HOF-1) with suitable pore cavities for highly efficient CO2/CO separation under dry and humid conditions.

7.
Nanoscale ; 13(35): 14980-14986, 2021 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-34533178

RESUMO

Photoalignment technology provides high potential for the manipulation of molecular orientations and has been widely used in liquid crystal displays. In this work, we align a luminescent film composite of CsPbBr3 nanowires (NWs) and liquid crystal molecules through photoalignment conducted on a PDMS template. We successfully define different orientations of CsPbBr3 NWs on the same substrate and the fluorescence micrographs clearly exhibit the orthogonal polarization direction of the two regions. On the basis of this research, we develop micro-photoalignment technology, which is promising for fabricating complex and precise nanostructures for photonic applications.

8.
Opt Lett ; 46(18): 4582-4584, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34525052

RESUMO

We present a novel, to the best of our knowledge, broadband and angle-insensitive nearly perfect absorber design composed of alternate silica and chromium layers. We show that by depositing a chromium nanofilm on a chromium substrate with a silica spacer, the absorption will significantly enhance from the visible to near-infrared. Then, another silica film is placed on the top of the layered structure as an antireflection coating, resulting in the broadband near-perfect absorption. We fabricate the proposed absorber by magnetron sputtering. The measured results show that our device has an average absorption over 97% in a wide range of wavelengths ranging from 350 to 1170 nm, and its absorption performance exhibits a good angular tolerance up to 50°. The presented absorber design offers a good prospect for large-scale and low-cost manufacturing of absorption-based optoelectronic devices.

9.
Nat Commun ; 12(1): 5072, 2021 08 20.
Artigo em Inglês | MEDLINE | ID: mdl-34417473

RESUMO

In vivo bioprinting has recently emerged as a direct fabrication technique to create artificial tissues and medical devices on target sites within the body, enabling advanced clinical strategies. However, existing in vivo bioprinting methods are often limited to applications near the skin or require open surgery for printing on internal organs. Here, we report a ferromagnetic soft catheter robot (FSCR) system capable of in situ computer-controlled bioprinting in a minimally invasive manner based on magnetic actuation. The FSCR is designed by dispersing ferromagnetic particles in a fiber-reinforced polymer matrix. This design results in stable ink extrusion and allows for printing various materials with different rheological properties and functionalities. A superimposed magnetic field drives the FSCR to achieve digitally controlled printing with high accuracy. We demonstrate printing multiple patterns on planar surfaces, and considering the non-planar surface of natural organs, we then develop an in situ printing strategy for curved surfaces and demonstrate minimally invasive in vivo bioprinting of hydrogels in a rat model. Our catheter robot will permit intelligent and minimally invasive bio-fabrication.


Assuntos
Bioimpressão , Cateteres , Imãs/química , Robótica , Animais , Linhagem Celular , Elasticidade , Condutividade Elétrica , Humanos , Hidrogéis/química , Fígado/diagnóstico por imagem , Ratos Sprague-Dawley , Suínos , Tomografia Computadorizada por Raios X , Viscosidade
10.
Materials (Basel) ; 14(15)2021 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-34361308

RESUMO

Hydrothermal carbonization (HTC) has been proved to be a promising technology for swine manure (SM) treatment. Currently, there is a lack of systematic understanding of the transformation characteristics of nutrient speciation in the HTC of SM. In this study, the speciation of the main nutrients (N/P/K) in SM-derived hydrochar produced at different reaction temperatures (200-280 °C) was investigated. The recovery of P (61.0-67.1%) in hydrochars was significantly higher than that of N (23.0-39.8%) and K (25.5-30.0%), and the increase in reaction temperature promoted the recovery of P and reduced the recovery of N. After the HTC treatment, the percentage of soluble/available P was reduced from 61.6% in raw SM to 4.0-23.9% in hydrochars, while that of moderately labile/slow-release P was improved from 29.2% in raw SM feedstock to 65.5-82.7%. An obvious reduction was also found in the amounts of available N (from 51.3% in raw SM feedstock to 33.0-40.5% in hydrochars). The percentages of slow-release N and residual N in hydrochars produced at 240 °C reached the maximum and minimum values (46.4% and 18.9%), respectively. A total of 49.5-58.3% of K retained in hydrochars was residual (invalid) potassium. From the perspective of the mobility and availability of N, P and K only, it was suggested that the HTC of SM should be carried out at 220-240 °C. Compared with the original SM, it is safer and more effective to use the SM-derived hydrochar as an organic fertilizer.

11.
J Transl Med ; 19(1): 326, 2021 07 31.
Artigo em Inglês | MEDLINE | ID: mdl-34332599

RESUMO

BACKGROUND: A novel and improved methodology is still required for the diagnosis of diabetic kidney disease (DKD). The aim of the present study was to identify novel biomarkers using extracellular vesicle (EV)-derived mRNA based on kidney tissue microarray data. METHODS: Candidate genes were identified by intersecting the differentially expressed genes (DEGs) and eGFR-correlated genes using the GEO datasets GSE30528 and GSE96804, followed by clinical parameter correlation and diagnostic efficacy assessment. RESULTS: Fifteen intersecting genes, including 8 positively correlated genes, B3GALT2, CDH10, MIR3916, NELL1, OCLM, PRKAR2B, TREM1 and USP46, and 7 negatively correlated genes, AEBP1, CDH6, HSD17B2, LUM, MS4A4A, PTN and RASSF9, were confirmed. The expression level assessment results revealed significantly increased levels of AEBP1 in DKD-derived EVs compared to those in T2DM and control EVs. Correlation analysis revealed that AEBP1 levels were positively correlated with Cr, 24-h urine protein and serum CYC and negatively correlated with eGFR and LDL, and good diagnostic efficacy for DKD was also found using AEBP1 levels to differentiate DKD patients from T2DM patients or controls. CONCLUSIONS: Our results confirmed that the AEBP1 level from plasma EVs could differentiate DKD patients from T2DM patients and control subjects and was a good indication of the function of multiple critical clinical parameters. The AEBP1 level of EVs may serve as a novel and efficacious biomarker for DKD diagnosis.


Assuntos
Diabetes Mellitus , Nefropatias Diabéticas , Vesículas Extracelulares , Biomarcadores , Carboxipeptidases , Nefropatias Diabéticas/diagnóstico , Nefropatias Diabéticas/genética , Taxa de Filtração Glomerular , Humanos , RNA Mensageiro/genética , Proteínas Repressoras
12.
Acta Pharmacol Sin ; 2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-34417574

RESUMO

Silicosis caused by inhalation of silica particles leads to more than ten thousand new occupational exposure-related deaths yearly. Exacerbating this issue, there are currently few drugs reported to effectively treat silicosis. Tetrandrine is the only drug approved for silicosis treatment in China, and despite more than decades of use, its efficacy and mechanisms of action remain largely unknown. Here, in this study, we established silicosis mouse models to investigate the effectiveness of tetrandrine of early and late therapeutic administration. To this end, we used multiple cardiopulmonary function test, as well as markers for inflammation and fibrosis. Moreover, using single cell RNA sequencing and transcriptomics of lung tissue and quantitative microarray analysis of serum from silicosis and control mice, our results provide a novel description of the target pathways for tetrandrine. Specifically, we found that tetrandrine attenuated silicosis by inhibiting both the canonical and non-canonical NLRP3 inflammasome pathways in lung macrophages. Taken together, our work showed that tetrandrine yielded promising results against silicosis-associated inflammation and fibrosis and further lied the groundwork for understanding its molecular targets. Our results also facilitated the wider adoption and development of tetrandirne, potentially accelerating a globally accepted therapeutic strategy for silicosis.

13.
Chemosphere ; 285: 131472, 2021 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-34265723

RESUMO

Microbial mediated iron cycling drives the biogeochemical cycling of carbon, nitrogen, sulfur, and phosphorus. However, the fate of the microbial community and the relative metabolic pathways in paddy soil after the addition of biogas slurry are poorly understood. In this study, the response of functional genes was investigated by growing one-season rice in paddy soils in a pot experiment. Seven treatments were prepared: 1) control (CK); 2) organic carbon (OC); 3) fertilizer (F); 4) 5% of biogas slurry (B05); 5) 10% of biogas slurry (B10); 6) 15% of biogas slurry (B15); 7) 20% of biogas slurry (B20). In the biogas slurry treatments, Geobacter increased more than in the other treatments during rice growth, which were structured by TOC. Particularly, in the B10 treatment, the relative abundance of Geobacter was 1.6 and 14.8 times higher than that of CK at the heading and mature stages, respectively. At the heading stage, the addition of biogas slurry and OC shifted the microbial phosphorus-transformation communities differently. There were no significant differences in the carbon, nitrogen, and sulfur metabolic pathways between the two treatments. At the mature stage, the carbon: nitrogen: phosphorus balance was significantly influenced by the regulation of functional gene expression and metabolic activities. These findings provide insight into the key factors affecting carbon, nitrogen, sulfur, phosphorus, and iron during rice growth after carbon inputs.

14.
Amino Acids ; 2021 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-34283311

RESUMO

Proline metabolic reprogramming is intimately involved in cancer progression. We recently identified a critical role of PINCH-1, a cell-extracellular matrix (ECM) adhesion protein whose expression is elevated in lung adenocarcinoma, in the promotion of proline biosynthesis, fibrosis and lung adenocarcinoma growth. How PINCH-1 promotes proline biosynthesis, however, was incompletely understood. In this study, we show that PINCH-1 promotes the expression of Δ1-pyrroline-5-carboxylate synthase (P5CS), a key enzyme that links glutamate metabolism to proline biosynthesis. Depletion of PINCH-1 from lung adenocarcinoma cells reduced the protein but not mRNA level of P5CS, resulting in down-regulation of the cellular level of P5C and cell proliferation. Treatment of the cells with protease inhibitor leupeptin effectively reversed PINCH-1 deficiency-induced reduction of the P5CS level. At the molecular level, PINCH-1, through its LIM2 domain, physically associated with P5CS in lung adenocarcinoma cells. Re-expression of wild type PINCH-1, but not that of the PINCH-1 LIM2 deletion mutant, in PINCH-1 deficient lung adenocarcinoma cells restored P5CS expression, proline biosynthesis and cell proliferation. Finally, P5CS expression, like that of PINCH-1, is elevated in human and mouse lung adenocarcinoma. Using a mouse model of lung adenocarcinoma in which PINCH-1 is conditionally ablated, we show that knockout of PINCH-1 from lung adenocarcinoma effectively reduced the P5CS level in vivo. Our results reveal an important role of PINCH-1 in the promotion of P5CS expression, which likely contributes to proline metabolic reprogramming and consequently lung adenocarcinoma progression.

15.
ACS Appl Mater Interfaces ; 13(30): 36147-36156, 2021 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-34289684

RESUMO

In this work, we proposed a fluorescence conversion layer with polarization characteristics to enhance UV polarization detection for the first time. To achieve this goal, the colloidal lead halide CsPbBr3 nanowires (NWs) with appropriate lengths were synthesized by the method of ultrasonication synthesis assisted by the addition of hydrobromic acid (HBr) ligands. By adding HBr, the properties of synthesized NWs are improved, and due to the controllable perovskite-stretched NWs, polymer composite films were fabricated, which can generate photoluminescence (PL) with strong polarization. The optimized stretched composite film can achieve a polarization degree of 0.42 and dichroism ratio (I∥/I⊥) of 2.49 at 520 nm. Based on this film, an imaging system with polarization-selective properties and efficient UV spectral conversion was developed. The spectrum conversion of 266 to 520 nm luminescence wavelength was realized and sensitive to the polarization of incoming 266 nm UV light. The experimental results also showed that the response after spectral conversion is greatly improved, and different responsivities can correspond to different polarization states. This imaging system overcomes the insufficiency of the conventional charge coupled device (CCD), which makes it difficult to receive the optical signal for high-quality UV imaging. The use of light conversion films with polarization characteristics for polarized UV imaging is of great significance for improving the detection of solar-blind UV bands and the recognition of military targets.

16.
J Am Chem Soc ; 143(35): 14147-14157, 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34288685

RESUMO

Sensing temperature at the subcellular level is of great importance for the understanding of miscellaneous biological processes. However, the development of sensitive and reliable organic fluorescent nanothermometers remains challenging. In this study, we report the fabrication of a novel organic fluorescent nanothermometer and study its application in temperature sensing. First of all, we synthesize a dual-responsive organic luminogen that can respond to the molecular state of aggregation and environmental polarity. Next, natural saturated fatty acids with sharp melting points as well as reversible and rapid phase transition are employed as the encapsulation matrix to correlate external heat information with the fluorescence properties of the luminogen. To apply the composite materials for biological application, we formulate them into colloidally dispersed nanoparticles by a technique that combines in situ surface polymerization and nanoprecipitation. As anticipated, the resultant zwitterionic nanothermometer exhibits sensitive, reversible, reliable, and multiparametric responses to temperature variation within a narrow range around the physiological temperature (i.e., 37 °C). Taking spectral position, fluorescence intensity, and fluorescence lifetime as the correlation parameters, the maximum relative thermal sensitivities are determined to be 2.15% °C-1, 17.06% °C-1, and 17.72% °C-1, respectively, which are much higher than most fluorescent nanothermometers. Furthermore, we achieve the multimodal temperature sensing of bacterial biofilms using these three complementary fluorescence parameters. Besides, we also fabricate a cationic form of the nanothermometer to facilitate efficient cellular uptake, holding great promise for studying thermal behaviors in biological systems.

17.
Chemistry ; 27(53): 13367-13375, 2021 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-34319625

RESUMO

Polyoxometalates (POMs)-based materials, with high theoretical capacities and abundant reversible multi-electron redox properties, are considered as promising candidates in lithium-ion storage. However, the poor electronic conductivity, low specific surface area and high solubility in the electrolyte limited their practical applications. Herein, a double-shelled hollow PMo12 -SiO2 @N-C nanofiber (PMo12 -SiO2 @N-C, where PMo12 is [PMo12 O40 ]3- , N-C is nitrogen-doped carbon) was fabricated for the first time by combining coaxial electrospinning technique, thermal treatment and electrostatic adsorption. As an anode material for LIBs, the PMo12 -SiO2 @N-C delivered an excellent specific capacity of 1641 mA h g-1 after 1000 cycles under 2 A g-1 . The excellent electrochemical performance benefited from the unique double-shelled hollow structure of the material, in which the outermost N-C shell cannot only hinder the agglomeration of PMo12 , but also improve its electronic conductivity. The SiO2 inner shell can efficiently avoid the loss of active components. The hollow structure can buffer the volume expansion and accelerate Li+ diffusion during lithiation/delithiation process. Moreover, PMo12 can greatly reduce charge-resistance and facilitate electron transfer of the entire composites, as evidenced by the EIS kinetics study and lithium-ion diffusion analysis. This work paves the way for the fabrication of novel POM-based LIBs anode materials with excellent lithium storage performance.

18.
J Ethnopharmacol ; 278: 114283, 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34098017

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Gegen Qinlian Decoction (GQD) (including: Puerariae lobatae (Willd.) Ohwi, radix; (short for Gengen) Glycyrrhiza uralensis Fisch., root and rhizome (short for Gancao), honeyed; Coptis chinensis Franch., rhizome (short for Huanglian); Scutellaria baicalensis Georgi, radix, boiled (short for S. baicalensis) has been widely used to treat inflammatory bowel disease (IBD) and colorectal cancer (CRC). To explore compatibility mechanism of GQD could be of advantage to investigate the complex principle of TCM, which might be conducive to the exploration of the modernization of TCM. AIM OF REVIEW: In this study, a strategy based on system pharmacology was constructed to uncover the multi-target regulation and compatibility mechanism of GQD on the Wnt signaling pathways. MATERIAL AND METHODS: The pharmacological network of GQD was constructed by TCMSP, DAVID, Uniprote database. The cell growth inhibitory effects of puerarin (PUE), wogonin (WOG), berberine (BER), and glycyrrhetinic acid (GLY) on SW480 cells were assessed using CCK-8 assay. The multi-target regulation and compatibility mechanism of combination PUE with GLY were examined by RNA-seq, HPLC-QQQ/MS, qRT- PCR and Western blot analysis. RESULTS: Network pharmacology analysis indicated that PUE, WOG, BER and GLY were the active components in GQD and had a synergistic effect on the targets of the Wnt signaling pathway. Additionally, pharmacological experiments revealed that WOG, BER, and GLY inhibited activity of colorectal cancer (CRC) cell lines SW480 cells, and that PUE only exhibited effective antitumour activity when combined with GLY. CTNNB1, CCND1 and SMAD4 were identified as synergistic targets inhibited by PUE-GLY. Moreover, PUE-GLY could influence the Wnt signaling pathway by upregulating GSK3B and downregulating CTNNB1 synergistically. It also showed that GLY could effectively increase the intracellular content of PUE based on HPLC-QQQ/MS analysis, and this process was achieved by influencing the targets of the membrane's pathway, such as cell adhesion molecules, focal adhesion, and tight junctions. CONCLUSION: GLY was revealed a multi-target mechanism, which could downregulate CTNNB1 as the active component and intervene in membrane proteins (CDH1, CADM1, ITGB2, ICAM1, ITGA1) as 'guide' in the formulae. Moreover, the mechanism of synergistic antitumour action of PUE (the active component of Monarch drug) and GLY (the active component of Guide drug) on the Wnt signaling pathway was explored systematically. It was a promising breakthrough for elucidating the scientific connotation of the compatibility of TCM formulae and provide a valuable and practicable methodology for clarifying the mechanisms of TCM.

19.
Nanoscale ; 13(26): 11360-11369, 2021 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-34096562

RESUMO

Emulating the biological behavior of the human brain with artificial neuromorphic devices is essential for the future development of human-machine interactive systems, bionic sensing systems and intelligent robotic systems. In this paper, artificial flexible transparent carbon nanotube synaptic transistors (F-CNT-STs) with signal transmission and emotional learning functions are realized by adopting the poly(vinyl alcohol) (PVA)/SiO2 proton-conducting electrolyte. Synaptic functions of biological synapses including excitatory and inhibitory behaviors are successfully emulated in the F-CNT-STs. Besides, synaptic plasticity such as spike-duration-dependent plasticity, spike-number-dependent plasticity, spike-amplitude-dependent plasticity, paired-pulse facilitation, short-term plasticity, and long-term plasticity have all been systematically characterized. Moreover, the F-CNT-STs also closely imitate the behavior of human brain learning and emotional memory functions. After 1000 bending cycles at a radius of 3 mm, both the transistor characteristics and the synaptic functions can still be implemented correctly, showing outstanding mechanical capability. The realized F-CNT-STs possess low operating voltage, quick response, and ultra-low power consumption, indicating their high potential to work in low-power biological systems and artificial intelligence systems. The flexible artificial synaptic transistor enables its potential to be generally applicable to various flexible wearable biological and intelligent applications.


Assuntos
Nanotubos de Carbono , Inteligência Artificial , Humanos , Dióxido de Silício , Sinapses , Transistores Eletrônicos
20.
J Environ Sci (China) ; 105: 56-63, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34130839

RESUMO

Mineral particles are ubiquitous in the atmosphere and exhibit an important effect on the photooxidation of volatile organic compounds (VOCs). However, the role of mineral particles in the photochemical oxidation mechanism of VOCs remains unclear. Hence, the photooxidation reactions of acrolein (ARL) with OH radical (OH) in the presence and absence of SiO2 were investigated by theoretical approach. The gas-phase reaction without SiO2 has two distinct pathways (H-abstraction and OH-addition pathways), and carbonyl-H-abstraction is the dominant pathway. In the presence of SiO2, the reaction mechanism is changed, i.e., the dominant pathway from carbonyl-H-abstraction to OH-addition to carbonyl C-atom. The energy barrier of OH-addition to carbonyl C-atom deceases 21.33 kcal/mol when SiO2 is added. Carbonyl H-atom of ARL is occupied by SiO2 via hydrogen bond, and carbonyl C-atom is activated by SiO2. Hence, the main product changes from H-abstraction product to OH-adduct in the presence of SiO2. The OH-adduct exhibits a thermodynamic feasibility to yield HO2 radical and carboxylic acid via the subsequent reactions with O2, with implications for O3 formation and surface acidity of mineral particles.


Assuntos
Radical Hidroxila , Dióxido de Silício , Atmosfera , Cinética , Minerais
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