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AIMS: Very Low Birthweight (VLBW) infants with neonatal Chronic Lung Disease (CLD) have been found to have functional impairment of the brainstem auditory pathway at term. This study investigated the functional status of the brainstem auditory pathway in VLBW infants with CLD after term for any abnormality. METHODS: Fifty-two VLBW infants were recruited at 50 weeks of Postconceptional Age: 25 with neonatal CLD and 27 without CLD. None had any other major complications to minimize confounding effects. Brainstem Auditory Evoked Responses were studied at 21â91/s click rates. RESULTS: Compared with those without CLD, VLBW infants with CLD had relatively shorter latencies of BAER waves I and III, associated with a slightly lower BAER threshold. Wave V latency and IâV interpeak interval did not differ significantly between the two groups of infants. The IâIII interval in infants with CLD was shorter than in those without CLD at 91/s clicks. However, the IIIâV interval was significantly longer than in those without CLD at all click rates (all p < 0.05). There were no significant differences in the amplitudes of BAER wave components between the two groups of infants. CONCLUSIONS: The main BAER abnormality in VLBW infants with CLD was a prolonged IIIâV interval. Auditory conduction is delayed or impaired at more central regions of the brainstem in CLD infants. After term central auditory function is adversely affected by neonatal CLD. Monitoring post-term change is required to provide valuable information for post-term care of CLD infants.
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Pneumopatias , Recém-Nascido , Lactente , Humanos , Adulto , Pneumopatias/complicações , Audição , Vias Auditivas , Potenciais Evocados Auditivos do Tronco Encefálico/fisiologia , Tronco EncefálicoRESUMO
Abstract Aims Very Low Birthweight (VLBW) infants with neonatal Chronic Lung Disease (CLD) have been found to have functional impairment of the brainstem auditory pathway at term. This study investigated the functional status of the brainstem auditory pathway in VLBW infants with CLD after term for any abnormality. Methods Fifty-two VLBW infants were recruited at 50 weeks of Postconceptional Age: 25 with neonatal CLD and 27 without CLD. None had any other major complications to minimize confounding effects. Brainstem Auditory Evoked Responses were studied at 21‒91/s click rates. Results Compared with those without CLD, VLBW infants with CLD had relatively shorter latencies of BAER waves I and III, associated with a slightly lower BAER threshold. Wave V latency and I‒V interpeak interval did not differ significantly between the two groups of infants. The I‒III interval in infants with CLD was shorter than in those without CLD at 91/s clicks. However, the III‒V interval was significantly longer than in those without CLD at all click rates (all p < 0.05). There were no significant differences in the amplitudes of BAER wave components between the two groups of infants. Conclusions The main BAER abnormality in VLBW infants with CLD was a prolonged III‒V interval. Auditory conduction is delayed or impaired at more central regions of the brainstem in CLD infants. After term central auditory function is adversely affected by neonatal CLD. Monitoring post-term change is required to provide valuable information for post-term care of CLD infants.
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Myelodysplastic syndrome (MDS) consists of a group of hematologic tumors that are derived from the clonal proliferation of hematopoietic stem cells, featuring abnormal hematopoietic cell development and ineffective hematopoiesis. Animal models are an important scientific research platform that has been widely applied in the research of human diseases, especially tumors. Animal models with MDS can simulate characteristic human genetic variations and tumor phenotypes. They also provide a reliable platform for the exploration of the pathogenesis and diagnostic markers of MDS as well as for a drug efficacy evaluation. This paper reviews the research status of three animal models and a new spontaneous mouse model with MDS.
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Neoplasias Hematológicas , Síndromes Mielodisplásicas , Animais , Camundongos , Humanos , Síndromes Mielodisplásicas/genética , Células-Tronco Hematopoéticas/patologia , Modelos Animais de Doenças , HematopoeseRESUMO
ABSTRACT Introduction: The ski jumping sequence can be divided into four steps: support, take-off, flight, and landing. Each stage has specific tasks and functions and is closely related, with the completion of each stage depending on the previous one. Objective: Explore the effectiveness of special strength training methods for ski sportsmen. Methods: Using the method of scientific literature review, expert interview, experience, and mathematical statistics to perform the special strength training for Chinese ski athletes, comparing the changes in the jump speed and take-off of the big jumps before and after 16 weeks of strength training with a specially developed protocol. Results: There were significant differences in take-off speed and air height before and after the experiment (P<0.05). The athletes in the experimental group significantly differed in takeoff speed before and after participation in the experiment (P=0.01). Conclusion: The experimental results fully evidence that by improving the rapid contraction strength of flexor and extensor muscles of the trunk and hip joints, modern abdominal center strength training methods are more effective than traditional methods. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução: A sequência de saltos de esqui pode ser dividida em quatro etapas: apoio, decolagem, voo e pouso. Cada etapa tem tarefas e funções específicas e está intimamente relacionada, sendo a conclusão de cada etapa dependente da etapa anterior. Objetivo: Explorar a eficácia dos métodos especiais de treinamento de força para esportistas em esqui. Métodos: Usando o método de análise científica literária, entrevista com especialistas, experiência e estatísticas matemáticas para realizar o treinamento de força especial para atletas chineses de esqui, comparando as mudanças na velocidade de salto e decolagem dos grandes saltos antes e depois de 16 semanas de treinamento de força com um protocolo especialmente desenvolvido. Resultados: Houve diferenças significativas na velocidade de decolagem e na altura aérea antes e depois do experimento (P<0,05). Os atletas do grupo experimental tiveram uma diferença muito significativa na velocidade de decolagem antes e depois da participação no experimento (P=0,01). Conclusão: Os resultados experimentais evidenciaram plenamente que ao melhorar a força de contração rápida dos músculos flexores e extensores das articulações do tronco e quadril, os métodos modernos de treinamento de força do centro abdominal são mais eficazes do que os métodos tradicionais de treinamento. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: La secuencia de salto de esquí puede dividirse en cuatro pasos: apoyo, despegue, vuelo y aterrizaje. Cada paso tiene tareas y funciones específicas y está estrechamente relacionado, siendo la conclusión de cada paso dependiente del anterior. Objetivo: Explorar la eficacia de los métodos especiales de entrenamiento de la fuerza para los deportistas de esquí. Métodos: Utilizando el método de revisión de la literatura científica, la entrevista a expertos, la experiencia y la estadística matemática para realizar el entrenamiento de fuerza especial para los atletas de esquí chinos, comparando los cambios en la velocidad de salto y el despegue de los grandes saltos antes y después de 16 semanas de entrenamiento de fuerza con un protocolo especialmente desarrollado. Resultados: Hubo diferencias significativas en la velocidad de despegue y la altura del aire antes y después del experimento (P<0,05). Los atletas del grupo experimental tuvieron una diferencia muy significativa en la velocidad de despegue antes y después de la participación en el experimento (P=0,01). Conclusión: Los resultados experimentales evidencian plenamente que, al mejorar la fuerza de contracción rápida de los músculos flexores y extensores de las articulaciones del tronco y la cadera, los métodos modernos de entrenamiento de la fuerza del núcleo abdominal son más eficaces que los métodos de entrenamiento tradicionales. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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ABSTRACT Introduction: Tennis is a competitive sport endowed with subtle movements, sophisticated and changeable techniques and tactics, and intense confrontation. It has high demands on the athlete's physical and psychological qualities. To win high-level professional tennis events, in addition to basic skills, comprehensive technical and tactical abilities, stable psychological quality, and flexible and innovative thinking, tennis players must also have exceptional physical fitness. Objective: Study the index system for evaluating the sport-specific skills of tennis athletes. Methods: 30 youth tennis training athletes were selected. Research methods such as literature, expert interview, questionnaire, and mathematical statistics were used to construct the fitness evaluation index of Chinese professional tennis players. Results: Professional tennis players' specific fitness assessment indexes include one first-level index, 14 second-level indexes, and 23 three-item indexes. Conclusion: Young athletes must strengthen their agile attack speed, explosive strength, core strength, and coordination. The individual indicators are weighted according to individual standards of physical training level. Thus, the five first-level indicators and specific fitness standards are established in this paper. The results of this research have guiding significance for the formulation and implementation of further tennis education and training plans. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução: O tênis é um esporte de competitividade dotado de movimentos sutis, técnicas e táticas sofisticadas e passíveis de mudança, e intenso confronto. Possui altas exigências quanto às qualidades físicas e psicológicas do atleta. Para vencerem eventos de tênis profissionais de alto nível, além de habilidades básicas, habilidades técnicas e táticas abrangentes, qualidade psicológica estável e pensamento flexível e inovador, os tenistas também devem ter uma aptidão física excepcional. Objetivo: Estudar o sistema de índice de avaliação das habilidades esportivas específicas dos atletas de tênis. Métodos: Selecionou-se 30 atletas de treinamento juvenil de tênis. Métodos de pesquisa como a literatura, entrevista com especialistas, questionário e estatísticas matemáticas foram utilizados para construir o índice de avaliação da aptidão física particular dos jogadores profissionais de tênis chineses. Resultados: Os índices específicos de avaliação da aptidão física dos tenistas profissionais incluem um índice de primeiro nível, 14 índices de segundo nível e 23 índices de três itens. Conclusão: É preciso que os jovens atletas fortaleçam sua velocidade de ataque ágil, força explosiva, força central e coordenação. Os indicadores individuais são ponderados de acordo com os padrões individuais de nível de treinamento físico. Desta forma, os cinco indicadores de primeiro nível e os padrões específicos de aptidão física são estabelecidos neste documento. Os resultados dessa pesquisa têm significado orientador para a formulação e implementação de planos de ensino e treinamento de tênis posteriores. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: El tenis es un deporte de competitividad dotado de movimientos sutiles, técnicas y tácticas sofisticadas y cambiantes, y un enfrentamiento intenso. Posee altas exigencias en cuanto a las cualidades físicas y psicológicas del atleta. Para ganar eventos de tenis profesional de alto nivel, además de las habilidades básicas, de las habilidades técnicas y tácticas completas, de la calidad psicológica estable y del pensamiento flexible e innovador, los tenistas también deben tener una forma física excepcional. Objetivo: Estudiar el sistema de índices para evaluar las habilidades deportivas específicas de los atletas de tenis. Métodos: Se seleccionaron 30 atletas juveniles de entrenamiento de tenis. Se utilizaron métodos de investigación como la literatura, la entrevista a expertos, el cuestionario y la estadística matemática para construir el índice de evaluación de la aptitud física particular de los tenistas profesionales chinos. Resultados: Los índices específicos de evaluación de la aptitud física de los tenistas profesionales incluyen un índice de primer nivel, 14 índices de segundo nivel y 23 índices de tres elementos. Conclusión: Es necesario que los jóvenes atletas refuercen su velocidad de ataque ágil, su fuerza explosiva, su fuerza central y su coordinación. Los indicadores individuales se ponderan en función de las normas individuales de nivel de entrenamiento físico. De este modo, en este documento se establecen los cinco indicadores de primer nivel y las normas específicas de aptitud. Los resultados de esta investigación tienen una importancia orientadora para la formulación y la aplicación de nuevos planes de educación y formación en materia de tenis. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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ABSTRACT Introduction: Tennis is highly technical, and any error in its players' strength training can exponentially impact the competition. A timely review and analysis of tennis players' biomechanics will help to better understand the training system and reveal the basis for future scientific sports training strategies. Objective: Analyzes the effect of different strength training methods to design the best protocol considering tennis players' speed and success rate. Methods: The effect of training on 40 tennis players is discussed. The volunteers were divided into the experimental and control group. The experimental group performed strength training for 40 minutes daily, thrice a week. The control group continued with conventional training. The trial lasted eight weeks. The speed and success rate of the training were tested before and after the experiment. Results: The experimental group players significantly improved serve speed (P<0.05). The success rate of the experimental group was also significantly improved (P<0.05). Conclusion: The proposed strength training significantly improved the success rates of speed in tennis players. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução: O tênis é um esporte altamente técnico e qualquer erro no treinamento de força de seus praticantes pode gerar impactos exponenciais na competição. Uma revisão e análise oportuna da biomecânica dos tenistas ajudará a entender melhor o sistema de treino e revelar as bases para a formulação de estratégias de treinamento esportivo científico no futuro. Objetivo: Analisa o efeito de diferentes métodos de treinamento de força para elaborar o melhor protocolo considerando a velocidade e a taxa de sucesso dos jogadores de tênis. Métodos: Discute-se o efeito do treinamento em 40 jogadores de tênis. Os voluntários foram divididos em grupo experimental e controle. O grupo experimental realizou um treinamento de força por 40 minutos diários, três vezes por semana. O grupo de controle continuou com o treino convencional. O ensaio durou oito semanas. A velocidade e a taxa de sucesso do treino foram testadas antes e depois do experimento. Resultados: Os jogadores do grupo experimental tiveram melhora significa na velocidade de saque (P<0,05). A taxa de sucesso do grupo experimental também foi significativamente aprimorada (P<0,05). Conclusão: O treinamento de força proposto conseguiu melhorar significativamente as taxas de sucesso a velocidade nos jogadores de tênis. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: El tenis es un deporte altamente técnico y cualquier error en el entrenamiento de la fuerza de sus jugadores puede generar impactos exponenciales en la cancha. Una revisión y un análisis oportunos de la biomecánica de los tenistas ayudarán a comprender mejor el sistema de entrenamiento y revelarán la base para la formulación de estrategias científicas de entrenamiento deportivo en el futuro. Objetivo: Analizar el efecto de diferentes métodos de entrenamiento de fuerza para diseñar el mejor protocolo teniendo en cuenta la velocidad y el índice de éxito de los tenistas. Métodos: Se analiza el efecto del entrenamiento en 40 tenistas. Los voluntarios se dividieron en grupo experimental y grupo de control. El grupo experimental realizó un entrenamiento de fuerza durante 40 minutos diarios, tres veces por semana. El grupo de control continuó con el entrenamiento convencional. La prueba duró ocho semanas. La velocidad y la tasa de éxito del entrenamiento se comprobaron antes y después del experimento. Resultados: Los jugadores del grupo experimental tuvieron una mejora significativa en la velocidad de saque (P<0,05). La tasa de éxito del grupo experimental también mejoró significativamente (P<0,05). Conclusión: El entrenamiento de fuerza propuesto fue capaz de mejorar significativamente las tasas de éxito la velocidad en los jugadores de tenis. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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BACKGROUND: To understand provider perceptions of the COVID-19 pandemic on priorities of severe pediatric traumatic brain injury (TBI) care across hospitals in South America. METHODS: Site principal investigators (PIs) from 17 hospitals in South America enrolled in the PEGASUS-Argentina randomized controlled trial completed questionnaires regarding order of tasks performed in the care of a typical pediatric patient with severe TBI before (2019) and during (2021) the COVID-19 pandemic. Acute care processes were examined by quintiles to identify early, mid, and late actions and were categorized and compared. Associations of hospital volume and subspecialty resource availability with prioritization of key process actions were examined. FINDING: Site PIs from 15 and 16 hospitals completed the surveys in 2019 and 2021, respectively, including 14 who completed both. Action category order was stable between 2019 and 2021 and were ranked in priorities as: initial encounter, primary survey, interventions and invasive monitors, diagnostics, medications, staff communication, then disposition (in 2019) or nutrition (in 2021). There was variation in specific action order between hospitals at both timepoints, with only a few initial encounter and disposition actions limited to a single quintile. There was no reported association between hospital volume or subspecialty resource availability with prioritization of key process actions. INTERPRETATION: Despite novel healthcare challenges presented by the COVID-19 pandemic, providers in South America perceived maintaining standard severe pediatric TBI care consistent with BTF guidelines. There was large variability in specific action order between individual hospitals reported.
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Lesões Encefálicas Traumáticas , COVID-19 , Lesões Encefálicas Traumáticas/epidemiologia , Lesões Encefálicas Traumáticas/terapia , COVID-19/epidemiologia , Criança , Hospitais , Humanos , Pandemias , América do Sul/epidemiologiaRESUMO
OBJECTIVE: This study aimed at the oral health problems of elderly patients with diabetes. A training course of integrated traditional Chinese and Western medicine was constructed, helping patients improve their oral health quality of life. METHODS: A randomized controlled prospective experimental study was conducted. A total of 190 elderly patients were divided randomly into an observation group and a control group with 95 cases in each. The control group received regular health education, while the observation group was based on the control group to implement the integrated experiential learning of traditional Chinese and Western medicine in small groups. The oral health knowledge, attitude, behavior, and blood glucose control status along with the oral health quality of life of the two groups were compared before the intervention and at 3-month postintervention. RESULTS: Three months after the intervention, the fasting blood glucose control and the 2-h postprandial blood glucose/glycosylated hemoglobin levels in the observation group were significantly better than in the control group, and the difference was statistically significant (p<0.05). The oral health quality of life in the observation group was significantly better than in the control group, and the difference was statistically significant (p<0.05). CONCLUSION: The small-group experiential learning model of integrated Chinese and Western medicine can promote the transformation of knowledge-beliefs-behaviors in elderly patients with diabetes, which is conducive to controlling blood sugar levels and improving the quality of oral health.
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Diabetes Mellitus , Saúde Bucal , Idoso , China , Diabetes Mellitus/terapia , Humanos , Medicina Tradicional Chinesa , Aprendizagem Baseada em Problemas , Estudos Prospectivos , Qualidade de VidaRESUMO
SUMMARY OBJECTIVE: This study aimed at the oral health problems of elderly patients with diabetes. A training course of integrated traditional Chinese and Western medicine was constructed, helping patients improve their oral health quality of life. METHODS: A randomized controlled prospective experimental study was conducted. A total of 190 elderly patients were divided randomly into an observation group and a control group with 95 cases in each. The control group received regular health education, while the observation group was based on the control group to implement the integrated experiential learning of traditional Chinese and Western medicine in small groups. The oral health knowledge, attitude, behavior, and blood glucose control status along with the oral health quality of life of the two groups were compared before the intervention and at 3-month postintervention. RESULTS: Three months after the intervention, the fasting blood glucose control and the 2-h postprandial blood glucose/glycosylated hemoglobin levels in the observation group were significantly better than in the control group, and the difference was statistically significant (p<0.05). The oral health quality of life in the observation group was significantly better than in the control group, and the difference was statistically significant (p<0.05). CONCLUSION: The small-group experiential learning model of integrated Chinese and Western medicine can promote the transformation of knowledge-beliefs-behaviors in elderly patients with diabetes, which is conducive to controlling blood sugar levels and improving the quality of oral health.
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Humanos , Idoso , Saúde Bucal , Diabetes Mellitus/terapia , Qualidade de Vida , China , Estudos Prospectivos , Aprendizagem Baseada em Problemas , Medicina Tradicional ChinesaRESUMO
OBJECTIVE: To investigate the clinical and genetic causes of neonatal unconjugated hyperbilirubinemia. STUDY DESIGN: We included 1412 neonates diagnosed with unconjugated hyperbilirubinemia (total serum bilirubin >95 percentile for age), from the China Neonatal Genomes Project between August 2016 and September 2019, in the current study. Clinical data and targeted panel sequencing data on 2742 genes including known unconjugated hyperbilirubinemia genes were analyzed. RESULTS: Among the 1412 neonates with unconjugated hyperbilirubinemia, 37% had severe unconjugated hyperbilirubinemia, with total serum bilirubin levels that met the recommendations for exchange transfusion. Known clinical causes were identified for 68% of patients. The most common clinical cause in the mild unconjugated hyperbilirubinemia group was infection (17%) and in the severe group was combined factors (21%, with infection combined with extravascular hemorrhage the most common). A genetic variant was observed in 55 participants (4%), including 45 patients with variants in genes associated with unconjugated hyperbilirubinemia and 10 patients with variants that were regarded as additional genetic findings. Among the 45 patients identified with unconjugated hyperbilirubinemia-related variants, the genes were mainly associated with enzyme deficiencies, metabolic/biochemical disorders, and red blood cell membrane defects. G6PD and UGT1A1 variants, were detected in 34 of the 45 patients (76%). CONCLUSIONS: Known clinical causes, which varied with bilirubin levels, were identified in approximately two-thirds of the patients. Genetic findings were identified in 4% of the patients, including in patients with an identified clinical cause, with G6PD and UGT1A1 being the most common genes in which variants were detected.
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Glucosefosfato Desidrogenase , Glucuronosiltransferase , Hiperbilirrubinemia Neonatal , Bilirrubina , China , Glucosefosfato Desidrogenase/genética , Glucuronosiltransferase/genética , Humanos , Hiperbilirrubinemia/genética , Hiperbilirrubinemia Neonatal/genética , Recém-NascidoRESUMO
Abstract Gut bacterial β-glucuronidase (GUS) can reactivate xenobiotics that exert enterohepatic circulation- triggered gastrointestinal tract toxicity. GUS inhibitors can alleviate drug-induced enteropathy and improve treatment outcomes. We evaluated the inhibitory effect of Polygonum cuspidatum Siebold & Zucc. and its major constituents against Escherichia coli GUS (EcGUS), and characterized the inhibitory mechanism of each of the components. Trans-resveratrol 4'-O-β-D-glucopyranoside (HZ-1) and (-)-epicatechin gallate (HZ-2) isolated from P. cuspidatum were identified as the key components and potent inhibitors. These two components displayed strong to moderate inhibitory effects on EcGUS, with Ki values of 9.95 and 1.95 μM, respectively. Results from molecular docking indicated that HZ-1 and HZ-2 could interact with the key residues Asp163, Ser360, Ile 363, Glu413, Glu504, and Lys 568 of EcGUS via hydrogen bonding. Our findings demonstrate the inhibitory effect of P. cuspidatum and its two components on EcGUS, which supported the further evaluation and development of P. cuspidatum and its two active components as novel candidates for alleviating drug-induced damage in the mammalian gut.
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Low temperature remarkably limits rubber tree (Hevea brasiliensis Muell. Arg.) growth, latex production, and geographical distribution, but the underlying mechanisms of Hevea brasiliensis cold stress response remain elusive. Here, we identified HbSnRK2.6 as a key component in ABA signaling functions in phytohormone abscisic acid (ABA)-regulated cold stress response in Hevea brasiliensis. Exogenous application of ABA enhances Hevea brasiliensis cold tolerance. Cold-regulated (COR) genes in the CBF pathway are upregulated by ABA. Transcript levels of all five HbSnRK2.6 members are significantly induced by cold, while HbSnRK2.6A, HbSnRK2.6B, and HbSnRK2.6C can be further activated by ABA under cold conditions. Additionally, HbSnRK2.6s are localized in the cytoplasm and nucleus, and can physically interact with HbICE2, a crucial positive regulator in the cold signaling pathway. Overexpression of HbSnRK2.6A or HbSnRK2.6B in Arabidopsis extensively enhances plant responses to ABA and expression of COR genes, leading to increased cold stress tolerance. Furthermore, HbSnRK2.6A and HbSnRK2.6B can promote transcriptional activity of HbICE2, thus, increasing the expression of HbCBF1. Taken together, we demonstrate that HbSnRK2.6s are involved in ABA-regulated cold stress response in Hevea brasiliensis by regulating transcriptional activity of HbICE2.
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Ácido Abscísico/farmacologia , Resposta ao Choque Frio , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Hevea/metabolismo , Proteínas de Plantas/metabolismo , Proteínas Quinases/metabolismo , Fatores de Transcrição/metabolismo , Hevea/efeitos dos fármacos , Hevea/genética , Reguladores de Crescimento de Plantas/farmacologia , Proteínas de Plantas/genética , Proteínas Quinases/genética , Fatores de Transcrição/genéticaRESUMO
A systematic study over different treatment conditions, including hydrothermal and acid-thermal, was successfully carried out to determine the most suitable conditions to enhance the textural properties and surface chemical composition of natural dolomite. The reconstruction of dolomite after various treatments enhanced the surface area by 4-5 times and diminished the pore diameter between 70% and 81% compared to the untreated parent dolomite. The Rietveld analysis of the X-ray diffraction (XRD) patterns revealed changes in the crystalline compositions after each treatment. When the treated dolomite was used as a catalyst to produce glycerol carbonate via a transesterification reaction of glycerol and dimethyl carbonate, the crystalline Ca(OH)2 concentration of the modified dolomite and the apparent glycerol carbonate formation rate (rgc) are well-correlated. The results suggest that an increase of the crystalline Ca(OH)2 concentration could be related with surface basicity at the weak and moderate strength sites that may lead to an increase in catalytic activity. The hydrothermal treated dolomite showed a selectivity of glycerol carbonate greater than 99% and rgc value 3.42 mmol/min·gcat, which was higher than that achieved on other samples. This study could aid to the proper selection of dolomite treatment for the desired crystalline composition, depending on the applications of this highly available mineral.
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OBJECTIVES: Extracellular vesicle microRNAs (EV-miRNAs) have been demonstrated to be reliable candidate biomarkers for clinical applications. However, the clinical application potential of serum EV-miR-215-5p for gastric cancer (GC) remains poorly understood. The goal of our study was to determine the efficacy of serum EV-miR-215-5p in predicting the prognosis of GC. METHODS: Blood samples were collected from 118 patients with GC, 60 patients with benign gastric disease and BGD and 70 healthy controls. The relative levels of serum EV-miR-215-5p were measured using quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: Compared to patients with BGD and normal controls, GC patients exhibited remarkably higher serum EV-miR-215-5p level, especially those with early tumor recurrence (ETR). Receiver operating characteristic (ROC) curve analysis showed that serum EV-miR-215-5p was able to distinguish GC patients from BGD patients or healthy controls and GC patients with ETR from those without ETR. In addition, increased serum EV-miR-215-5p levels were notably correlated with invasive depth, TNM stage, and lymph node metastasis. Moreover, serum EV-miR-215-5p levels were greatly decreased after surgical treatment, but increased at the time of ETR. Survival analysis showed that patients with higher serum EV-miR-215-5p had shorter survival. Furthermore, serum EV-miR-215-5p was an independent risk factor for GC. CONCLUSIONS: Serum EV-miR-215-5p might be a novel biomarker for predicting ETR and prognosis of GC.
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Vesículas Extracelulares , MicroRNAs , Neoplasias Gástricas , Biomarcadores Tumorais , Humanos , Recidiva Local de Neoplasia , Prognóstico , Neoplasias Gástricas/genéticaRESUMO
OBJECTIVES: Extracellular vesicle microRNAs (EV-miRNAs) have been demonstrated to be reliable candidate biomarkers for clinical applications. However, the clinical application potential of serum EV-miR-215-5p for gastric cancer (GC) remains poorly understood. The goal of our study was to determine the efficacy of serum EV-miR-215-5p in predicting the prognosis of GC. METHODS: Blood samples were collected from 118 patients with GC, 60 patients with benign gastric disease and BGD and 70 healthy controls. The relative levels of serum EV-miR-215-5p were measured using quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: Compared to patients with BGD and normal controls, GC patients exhibited remarkably higher serum EV-miR-215-5p level, especially those with early tumor recurrence (ETR). Receiver operating characteristic (ROC) curve analysis showed that serum EV-miR-215-5p was able to distinguish GC patients from BGD patients or healthy controls and GC patients with ETR from those without ETR. In addition, increased serum EV-miR-215-5p levels were notably correlated with invasive depth, TNM stage, and lymph node metastasis. Moreover, serum EV-miR-215-5p levels were greatly decreased after surgical treatment, but increased at the time of ETR. Survival analysis showed that patients with higher serum EV-miR-215-5p had shorter survival. Furthermore, serum EV-miR-215-5p was an independent risk factor for GC. CONCLUSIONS: Serum EV-miR-215-5p might be a novel biomarker for predicting ETR and prognosis of GC.
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Humanos , Neoplasias Gástricas/genética , MicroRNAs , Vesículas Extracelulares , Prognóstico , Biomarcadores Tumorais , Recidiva Local de NeoplasiaRESUMO
OBJECTIVES: Extracellular vesicle microRNAs (EV-miRNAs) have been demonstrated to be reliable candidate biomarkers for clinical applications. However, the clinical application potential of serum EV-miR-215-5p for gastric cancer (GC) remains poorly understood. The goal of our study was to determine the efficacy of serum EV-miR-215-5p in predicting the prognosis of GC. METHODS: Blood samples were collected from 118 patients with GC, 60 patients with benign gastric disease and BGD and 70 healthy controls. The relative levels of serum EV-miR-215-5p were measured using quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: Compared to patients with BGD and normal controls, GC patients exhibited remarkably higher serum EV-miR-215-5p level, especially those with early tumor recurrence (ETR). Receiver operating characteristic (ROC) curve analysis showed that serum EV-miR-215-5p was able to distinguish GC patients from BGD patients or healthy controls and GC patients with ETR from those without ETR. In addition, increased serum EV-miR-215-5p levels were notably correlated with invasive depth, TNM stage, and lymph node metastasis. Moreover, serum EV-miR-215-5p levels were greatly decreased after surgical treatment, but increased at the time of ETR. Survival analysis showed that patients with higher serum EV-miR-215-5p had shorter survival. Furthermore, serum EV-miR-215-5p was an independent risk factor for GC. CONCLUSIONS: Serum EV-miR-215-5p might be a novel biomarker for predicting ETR and prognosis of GC.
Assuntos
Humanos , Neoplasias Gástricas/genética , MicroRNAs , Vesículas Extracelulares , Prognóstico , Biomarcadores Tumorais , Recidiva Local de NeoplasiaRESUMO
OBJECTIVE: This study aimed to investigate the impact of post-thoracotomy analgesia with dexmedetomidine and morphine on immunocytes. METHODS: A total of 118 patients with post-thoracotomy Patient-Controlled Intravenous Analgesia (PCIA) in our hospital from March 2016 to July 2018 were randomly selected and divided into the Composite (COM) Group (57 patients administered with dexmedetomidine [1.0 µg.kg-1 body weight] and morphine [0.48 mg.kg-1 body weight]) and the Morphine (MOR) Group (61 patients administered with morphine [0.48 mg.kg-1]). The values of lymphocyte subsets (CD3+, CD4+, and CD8+) and Natural Killer cells in the peripheral blood of these two groups were detected by FACSCalibur flow cytometry at different time points (before anesthesia induction [T0], immediately after tracheal extubation [T1], 12 hours after surgery [T2], 24 hours after surgery [T3], 48 hours after surgery [T4], 72 hours after surgery [T5], and 7 days after surgery [T6]). The doses of morphine at T3 to T5 and the adverse reactions between the two groups were also recorded and compared. RESULTS: The CD3+ level and the CD4+/CD8+ ratio at T2 to T5 and the CD4+ level and NK cells at T3 to T5 were significantly higher in the COM Group than in the MOR Group (p < 0.05). The postoperative morphine dose and the incidence of postoperative itching, nausea, and vomiting were significantly lower in the COM Group than in the MOR Group (p < 0.05). CONCLUSIONS: Dexmedetomidine combined with morphine for post-thoracotomy PCIA can improve the function of immunocytes, reduce morphine consumption, and reduce the adverse reactions during analgesia induction.
Assuntos
Analgesia Controlada pelo Paciente , Analgésicos não Narcóticos/farmacologia , Analgésicos Opioides/farmacologia , Dexmedetomidina/farmacologia , Células Matadoras Naturais/efeitos dos fármacos , Subpopulações de Linfócitos/efeitos dos fármacos , Morfina/farmacologia , Dor Pós-Operatória/tratamento farmacológico , Toracotomia , Adulto , Analgésicos não Narcóticos/uso terapêutico , Analgésicos Opioides/uso terapêutico , Dexmedetomidina/uso terapêutico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Morfina/uso terapêuticoRESUMO
Abstract Objective This study aimed to investigate the impact of post-thoracotomy analgesia with dexmedetomidine and morphine on immunocytes. Methods A total of 118 patients with post-thoracotomy Patient-Controlled Intravenous Analgesia (PCIA) in our hospital from March 2016 to July 2018 were randomly selected and divided into the Composite (COM) Group (57 patients administered with dexmedetomidine [1.0 µg.kg-1 body weight] and morphine [0.48 mg.kg-1 body weight]) and the Morphine (MOR) group (61 patients administered with morphine [0.48 mg.kg-1]). The values of lymphocyte subsets (CD3+, CD4+, and CD8+) and Natural Killer cells in the peripheral blood of these two groups were detected by FACSCalibur flow cytometry at different time points (before anesthesia induction [T0], immediately after tracheal extubation [T1], 12 hours after surgery [T2], 24 hours after surgery [T3], 48 hours after surgery [T4], 72 hours after surgery [T5], and 7 days after surgery [T6]). The doses of morphine at T3 to T5 and the adverse reactions between the two groups were also recorded and compared. Results The CD3+ level and the CD4+/CD8+ ratio at T2 to T5 and the CD4+ level and NK cells at T3 to T5 were significantly higher in the COM Group than in the MOR Group (p< 0.05). The postoperative morphine dose and the incidence of postoperative itching, nausea, and vomiting were significantly lower in the COM Group than in the MOR Group (p< 0.05). Conclusions Dexmedetomidine combined with morphine for post-thoracotomy PCIA can improve the function of immunocytes, reduce morphine consumption, and reduce the adverse reactions during analgesia induction.
Resumo Objetivo Estudar o impacto em linfócitos causado pelo uso da dexmedetomidina associada à morfina para analgesia pós-toracotomia. Método Um total de 118 pacientes utilizando Analgesia Intravenosa Controlada pelo Paciente (AICP) pós-toracotomia em nosso hospital, de março de 2016 a julho de 2018, foram selecionados aleatoriamente e divididos em dois grupos: o Grupo Combinado [COM, 57 pacientes que receberam dexmedetomidina (1,0 µg.kg-1 de peso corpóreo) associada à morfina (0,48 mg.kg-1 de peso corpóreo)] e o Grupo Morfina [MOR, 61 pacientes, que receberam somente morfina (0,48 mg.kg-)]. Os valores dos subconjuntos de linfócitos (CD3+, CD4+ e CD8+) e das células NK no sangue periférico desses dois grupos foram medidos por citometria de fluxo FACSCalibur em diferentes momentos do estudo [antes da indução anestésica (T0), imediatamente após extubação traqueal (T1), 12 horas após a cirurgia (T2), 24 horas após a cirurgia (T3), 48 horas após a cirurgia (T4), 72 horas após a cirurgia (T5) e 7 dias após a cirurgia (T6)]. As doses de morfina do momento T3 ao T5 e as reações adversas entre os dois grupos também foram registradas e comparadas. Resultados O nível de CD3+ e a razão CD4+/CD8+ de T2 a T5, e o nível de CD4+ e as células NK de T3 a T5 do Grupo COM foram significantemente maiores (p< 0,05) quando comparados ao Grupo MOR. A dose de morfina no pós-operatório e a incidência de prurido, náusea e vômito no pós-operatório foram significantemente menores no grupo MOR (p< 0,05). Conclusões Dexmedetomidina combinada com morfina para AICP no período pós-toracotomia pode melhorar a função dos linfócitos, reduzir o consumo de morfina e diminuir reações adversas durante a analgesia.
Assuntos
Humanos , Masculino , Feminino , Adulto , Dor Pós-Operatória/tratamento farmacológico , Toracotomia , Células Matadoras Naturais/efeitos dos fármacos , Analgesia Controlada pelo Paciente , Subpopulações de Linfócitos/efeitos dos fármacos , Analgésicos não Narcóticos/farmacologia , Dexmedetomidina/farmacologia , Analgésicos Opioides/farmacologia , Morfina/farmacologia , Analgésicos não Narcóticos/uso terapêutico , Dexmedetomidina/uso terapêutico , Analgésicos Opioides/uso terapêutico , Pessoa de Meia-Idade , Morfina/uso terapêuticoRESUMO
Biofertilizer Ning shield was composed of different strains of plant growth promotion bacteria. In this study, the plant growth promotion and root-knot nematode disease control potential on Trichosanthes kirilowii in the field were evaluated. The application of Ning shield significantly reduced the diseases severity caused by Meloidogyne incognita, the biocontrol efficacy could reached up to 51.08%. Ning shield could also promote the growth of T. kirilowii in the field by increasing seedling emergence, height and the root weight. The results showed that the Ning shield could enhance the production yield up to 36.26%. Ning shield could also promote the plant growth by increasing the contents of available nitrogen, phosphorus, potassium and organic matter, and increasing the contents of leaf chlorophyll and carotenoid pigment. Moreover, Ning shield could efficiently enhance the medicinal compositions of Trichosanthes, referring to the polysaccharides and trichosanthin. Therefore, Ning shield is a promising biofertilizer, which can offer beneficial effects to T. kirilowii growers, including the plant growth promotion, the biological control of root-knot disease and enhancement of the yield and the medicinal quality.(AU)
RESUMO
Abstract Biofertilizer Ning shield was composed of different strains of plant growth promotion bacteria. In this study, the plant growth promotion and root-knot nematode disease control potential on Trichosanthes kirilowii in the field were evaluated. The application of Ning shield significantly reduced the diseases severity caused by Meloidogyne incognita, the biocontrol efficacy could reached up to 51.08%. Ning shield could also promote the growth of T. kirilowii in the field by increasing seedling emergence, height and the root weight. The results showed that the Ning shield could enhance the production yield up to 36.26%. Ning shield could also promote the plant growth by increasing the contents of available nitrogen, phosphorus, potassium and organic matter, and increasing the contents of leaf chlorophyll and carotenoid pigment. Moreover, Ning shield could efficiently enhance the medicinal compositions of Trichosanthes, referring to the polysaccharides and trichosanthin. Therefore, Ning shield is a promising biofertilizer, which can offer beneficial effects to T. kirilowii growers, including the plant growth promotion, the biological control of root-knot disease and enhancement of the yield and the medicinal quality.