Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 190
Filtrar
1.
Blood Adv ; 3(21): 3406-3418, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31714962

RESUMO

Thrombocytopenia is associated with life-threatening bleeding and is common in myelodysplastic syndromes (MDS). Robust molecular prognostic biomarkers need to be developed to improve clinical decision making for patients with MDS with thrombocytopenia. Wilms tumor 1 (WT1) and preferentially expressed antigen in melanoma (PRAME) are promising immunogenic antigen candidates for immunotherapy, and their clinical effects on patients with MDS with thrombocytopenia are still not well understood. We performed a multicenter observational study of adult patients with MDS with thrombocytopenia from 7 different tertiary medical centers in China. We examined bone marrow samples collected at diagnosis for WT1 and PRAME transcript levels and then analyzed their prognostic effect for patients with MDS with thrombocytopenia. In total, we enrolled 1110 patients diagnosed with MDS with thrombocytopenia. Overexpression of WT1 and PRAME was associated with elevated blast percentage, worse cytogenetics, and higher Revised International Prognostic Scoring System (IPSS-R) risk. Further, both WT1 and PRAME overexpression were independent poor prognostic factors for acute myeloid leukemia evolution, overall survival, and progression-free survival. Together, the 2 genes overexpression identified a population of patients with MDS with substantially worse survival. On the basis of WT1 and PRAME transcript levels, patients with MDS with IPSS-R low risk were classified into 2 significantly divergent prognostic risk groups: a low-favorable group and a low-adverse group. The low-adverse group had survival similar to that of patients in the intermediate-risk group. Our study demonstrates that the evaluation of WT1/PRAME transcript analysis may improve the prognostication precision and better risk-stratify the patients.

2.
PLoS One ; 14(10): e0224089, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31622447

RESUMO

Population variation in disease and other phenotype are partly attributed to single nucleotide polymorphisms (SNPs) in the human genome. Due to selection pressure, two individuals from the same ancestral population have more genetic similarity compared to individuals from further geographic regions. Here, we elucidated the genomic population differentiation pattern, by interrogating >22,000,000 SNPs. Majority of population-differentiated (pd) SNPs (~95%), including the potentially functional (pf) (~84%) subset reside in non-genic regions, compared to the proportion of all SNPs (58%) found in non-genic regions. This suggests that differences between populations are more likely due to differences in gene regulation rather than protein function. Actin Cytoskeleton, Axonal Guidance and Protein Kinase A signaling pathways are enriched with genes carrying at least three pdSNPs (enriched pdGenes), while Antigen Presentation, Hepatic Fibrosis and Huntington Disease Signalling pathways are over-represented by enriched pf-pdGenes. An inverse correlation between chromosome size and the proportion of pd-/pf-pdSNPs was observed. Smaller chromosomes have relatively more of such SNPs including genes carrying these SNPs. Genes associated with common diseases and enriched with these pd-/pfpdSNPs are localized to 11 different chromosomes, with immune-related disease pd/pf-pdGenes mainly residing in chromosome 6 while neurological disease pd/pf-pdGenes residing in smaller chromosomes including chromosome 21/22. The associated diseases were reported to show population differences in incidence, severity and/or etiology. In summary, this study highlights the non-sporadic nature of population differentiation footprint in the human genome, which can potentially lead to the identification of genomic regions that play roles in the manifestation of phenotypic differences, including in disease predisposition and drug response.

3.
Pharmacogenomics J ; 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31578463

RESUMO

Drug response variations amongst different individuals/populations are influenced by several factors including allele frequency differences of single nucleotide polymorphisms (SNPs) that functionally affect drug-response genes. Here, we aim to identify drugs that potentially exhibit population differences in response using SNP data mining and analytics. Ninety-one pairwise-comparisons of >22,000,000 SNPs from the 1000 Genomes Project, across 14 different populations, were performed to identify 'population-differentiated' SNPs (pdSNPs). Potentially-functional pdSNPs (pf-pdSNPs) were then selected, mapped into genes, and integrated with drug-gene databases to identify 'population-differentiated' drugs enriched with genes carrying pf-pdSNPs. 1191 clinically-approved drugs were found to be significantly enriched (Z > 2.58) with genes carrying SNPs that were differentiated in one or more population-pair comparisons. Thirteen drugs were found to be enriched with such differentiated genes across all 91 population-pairs. Notably, 82% of drugs, which were previously reported in the literature to exhibit population differences in response were also found by this method to contain a significant enrichment of population specific differentiated SNPs. Furthermore, drugs with genetic testing labels, or those suspected to cause adverse reactions, contained a significantly larger number (P < 0.01) of population-pairs with enriched pf-pdSNPs compared with those without these labels. This pioneering effort at harnessing big-data pharmacogenomics to identify 'population differentiated' drugs could help to facilitate data-driven decision-making for a more personalized medicine.

4.
Dentomaxillofac Radiol ; : 20190253, 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31559846

RESUMO

Uremic leontiasis ossea (ULO), which occurs in the craniomaxillofacial region, is a sign of terminal stage osteitis fibrosa cystica or brown tumors and primarily caused by secondary hyperparathyroidism induced by renal failure. Pathophysiological changes include osteoclasts or osteoblasts proliferation, bone resorption, bone decalcification, and connective tissue proliferation. In this paper, we report a case of a 24-year-old female patient, who was diagnosed with ULO and presented with multiple facial swellings. Imaging features included zonal patterns with alternating rings of hypo- and hyperattenuated craniomaxillofacial bones, and diffused mixed sclerotic tissues with lytic changes in CT imaging. T1 weighted image and T2 weighted image in MRI were characterized by alternating rings of low and intermediate signal intensity patterns. To the best of our knowledge, this case is the first example of pathologically proved ULO with maxillofacial MRI.

5.
Sci Rep ; 9(1): 13046, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31506455

RESUMO

The fabella is a sesamoid bone of the knee that can degenerate in some patients with osteoarthritis. The purpose of this study was to examine the prevalence and degeneration grades of fabellae in the Chinese population and to analyse their relationships with subject ages and knee osteoarthritis grades. The anteroposterior and lateral knee roentgenograms of 1150 subjects were recruited from the institutional database. The Kellgren-Lawrence scoring system was used to evaluate knee osteoarthritis. The degeneration grades of fabellae were scored in lateral roentgenograms by screening their shapes, sizes, subchondral sclerosis and osteophyte formation. The prevalence and degeneration of fabellae among ages, genders and knee sides were analysed by the Pearson Chi-Square test, and their relationships with knee osteoarthritis were analysed by the Spearman nonparametric correlation test. The overall prevalence of fabellae was 48.6% in 1359 knees. There was no significant difference in fabellar prevalence between the two sides (χ² = 0.025, P = 0.87437) and genders (χ² = 3.647, P = 0.05617), while the prevalence increased with the increasing ages of the subjects (χ² = 213.868, P < 0.001). The fabellar degeneration grades were correlated with age (r = 0.5288, P < 0.001) and knee osteoarthritis scores (r = 0.6892, P < 0.001). These results suggested that the fabellar prevalence and degeneration grades were correlated with age and knee osteoarthritis scores.

6.
Phys Med ; 65: 53-58, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31430587

RESUMO

PURPOSE: It is still not conclusive which four-dimensional computed tomography (4DCT)-based ventilation imaging algorithm is most accurate and efficient. In this study, we proposed a simplified algorithm (VIAAVG) which only requires the average computed tomography (AVG CT) as input, and quantitatively compared its accuracy and efficiency with three other popular algorithms. MATERIAL AND METHODS: Fifty patients with lung or esophageal cancer who underwent radiotherapy were enrolled. Single photon emission computed tomography (SPECT) ventilation images (VI-SPECT) and 4DCT were acquired 1-3 days before the first treatment session. The end of exhalation and the end of inhalation CT were registered to derive deformable vector field (DVF) using MIMvista. 4DCT-based ventilation images (CTVI) were first calculated respectively by means of four algorithms (VIAJAC, VIAHU, VIAPRO and VIAAVG). The computation times were compared using paired t-test. The corresponding CTVIs (CTVIJAC, CTVIHU, CTVIPRO and CTVIAVG) and VI-SPECT were segmented into three equal sub-volumes (high, medium and low function lung, respectively) after smoothing and normalization. The Dice Similarity Coefficients (DSCs) were calculated for each sub-volume between each CTVI and VI-SPECT. The average DSCs for high, medium and low function lung in different CTVIs for each patient were compared using paired t-test. RESULTS: The mean DSCs for CTVIJAC, CTVIHU, CTVIPRO and CTVIAVG were 0.3255, 0.4465, 0.5865 and 0.5958, respectively. The average computation times for CTVIJAC, CTVIHU, CTVIPRO and CTVIAVG were 18.3 s, 24.2 s, 144.8 s and 15.0 s. CONCLUSION: VIAAVG is available for clinical use because of its high accuracy, improved efficiency and less input requirement compared to the other algorithms.

7.
Cancer Commun (Lond) ; 39(1): 43, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31307548

RESUMO

Lung cancer is the leading cause of cancer mortality worldwide. Dendritic cells (DCs) are the key factors providing protective immunity against lung tumors and clinical trials have proven that DC function is reduced in lung cancer patients. It is evident that the immunoregulatory network may play a key role in the failure of the immune response to terminate tumors. Lung tumors likely employ numerous strategies to suppress DC-based anti-tumor immunity. Here, we summarize the recent advances in our understanding on lung tumor-induced immunosuppression in DCs, which affects the initiation and development of T-cell responses. We also describe which existing measures to restore DC function may be useful for clinical treatment of lung tumors. Furthering our knowledge of how lung cancer cells alter DC function to generate a tumor-supportive environment will be essential in order to guide the design of new immunotherapy strategies for clinical use.

8.
Radiat Oncol ; 14(1): 106, 2019 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-31196118

RESUMO

BACKGROUND: Consistent results are lacking as regards the comparative effectiveness of simultaneous integrated boost intensity-modulated radiotherapy (SIB-IMRT) versus conventional intensity-modulated radiotherapy in patients with locally advanced non-small-cell lung cancer (LA-NSCLC). Therefore, we conducted a retrospective analysis to demonstrate the role of SIB-IMRT for patients. METHODS: Patients who had histologically confirmed NSCLC, stage III disease and received thoracic IMRT between 2014 and 2016 were retrospectively reviewed. The survival, toxicities and dose to organs at risk (OAR) were compared among patients irradiated with different techniques. The SIB-IMRT plans were designed to deliver 45-59.4Gy (median: 50.4Gy) to PTV while simultaneously delivering 50-70Gy (median: 59.92Gy) to PGTV. As for conventional IMRT plans, a total dose of 50-70Gy (median: 60Gy) was delivered to PTV. RESULTS: 426 patients with stage III NSCLC were eligible for analysis, including 128 with SIB-IMRT and 298 with conventional IMRT. The SIB-IMRT group had more stage IIIB disease (69.5% vs. 53%, P = 0.002), larger planning treatment volumes (median: 504 ml vs. 402 ml, P<0.001), and a larger planning treatment volume/volume of lung ratio (median, 0.18 vs. 0.12, P<0.001). The median OS of the SIB-IMRT and conventional IMRT groups were 34.5 and 31.7 months, with the 2-year rate of 60.4 and 59%, respectively (P = 0.797). No difference in PFS, LRFS or DMFS was observed between the two techniques. Patients treated with SIB-IMRT got similar lung and esophageal toxicities versus those with conventional IMRT. CONCLUSIONS: SIB-IMRT may be an effective and safe option for patients with locally advanced NSCLC, especially for those with large mass or wide lymph node metastasis.

9.
Food Chem ; 293: 169-177, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31151598

RESUMO

Antioxidant molecules in honey contributed to various biological effects, but antioxidant components markers in honey are required to be investigated further. Phenolic compounds, flavonoids and free amino acids were analyzed using UPLC-MS/MS and HPLC-FLD from 39 honey samples, in which fennel honey was firstly investigated. Based on the quantitative composition-activity relationship, the cellular antioxidant activity (CAA) assay of various honeys is closely related with the interaction of some phenolic compounds (isoferulic acid, 3,4-dihydroxy benzoic acid, 4-hydroxy benzoic acid, chlorogenic acid, caffeic acid, gallic acid, cryptochlorogenic acid, p-coumaric acid, salicylic acid), flavonoids (isosakuranetin, sakuranetin, pinocembrin, vitexin, taxifolin, galangin, luteolin, chrysin) and free amino acids (Tyr, Gly, Ile, Glu, Val, Phe, Leu, Asp, His, Pro, Ala). The results would be beneficial for the understanding of the nutritional values, exploitation and utilization of honeys with different floral origins, further contributable to the market development and consumption choice of honey.


Assuntos
Antioxidantes/análise , Mel/análise , Aminoácidos/análise , Aminoácidos/química , Antioxidantes/química , Cromatografia Líquida de Alta Pressão/métodos , Flavonoides/análise , Flavonoides/química , Análise dos Mínimos Quadrados , Fenóis/análise , Fenóis/química , Espectrometria de Massas em Tandem
10.
Ann Hematol ; 98(7): 1733-1742, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31053879

RESUMO

Hepatic sinusoidal obstruction syndrome (SOS) has been rarely studied after haploidentical donor (HID) allogeneic hematopoietic stem cell transplantation (allo-HSCT). We performed a retrospective multicentre study on patients with SOS after allo-HSCT in China. The incidence, risk factors, and outcomes were compared between HID HSCT and matched related donor (MRD) HSCT. SOS developed in 0.4% of patients (HIDs: 0.4%, MRDs: 0.5%, p = 0.952) at a median time of 21.50 days (range, 1-55) after allo-HSCT (HIDs: 24 days, MRDs: 20 days, p = 0.316). For patients diagnosed with SOS, the 2-year cumulative incidence of relapse was 22.7% and 22.4% in patients receiving HID and MRD transplantation, respectively (p = 0.584). Overall survival (OS) at 2 year was 10.4% and 38.5% in the two groups (p = 0.113). The transplant-related mortality (TRM) at 100 days was 60.9% in the HID group and 38.5% in the MRD group (p = 0.178). According to the multivariate analyses, significant independent risk factors for the occurrence of SOS were delayed platelet engraftment (p = 0.007) and advanced disease status at the time of HSCT (p = 0.009). The outcomes of SOS after HID HSCT are similar to those after MRD HSCT.


Assuntos
Transplante de Células-Tronco Hematopoéticas , Hepatopatia Veno-Oclusiva , Doadores de Tecidos , Condicionamento Pré-Transplante , Adolescente , Adulto , Aloenxertos , Criança , China/epidemiologia , Feminino , Seguimentos , Hepatopatia Veno-Oclusiva/epidemiologia , Hepatopatia Veno-Oclusiva/etiologia , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco
11.
Chin Med J (Engl) ; 132(13): 1563-1571, 2019 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-31058667

RESUMO

BACKGROUND: Nasopharyngeal carcinoma (NPC) is sensitive to radiotherapy (RT). However, neurocognitive complications such as memory loss and learning and attention deficits emerge in the survivors of NPC who received RT. It remains unclear how radiation affects patient brain function. This pilot study aimed at finding cerebral functional alterations in NPC patients who have received RT. METHODS: From September 2014 to December 2016, 42 individuals, including 22 NPC patients and 20 normal volunteer controls in National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences, and Peking Union Medical College, were recruited in this study. All patients received resting-state functional magnetic resonance imaging scans and neurocognitive tests 1 day before the initiation of RT (baseline) and 1 day after the completion of RT; the 20 normal controls were also subjected to the same scans and tests. The amplitude of the low-frequency fluctuations (ALFF) in blood oxygen level-dependent signals and functional connectivity (FC) were used to characterize cerebral functional changes. Independent t test, paired t test, and analysis of variances were used to obtain statistical significance across groups. RESULTS: After RT, NPC patients showed significantly decreased ALFF values in the calcarine sulcus, lingual gyrus, cuneus, and superior occipital gyrus and showed significantly reduced FC mainly in the default mode network (P < 0.05, corrected by AlphaSim). Relative to the controls, ALFF was decreased in the lingual gyrus, calcarine sulcus, cingulate cortex, medial prefrontal gyrus (P < 0.05, corrected by AlphaSim), and FC reduction was found in multiple cerebellar-cerebral regions, including the cerebellum, parahippocampus, hippocampus, fusiform gyrus, inferior frontal gyrus, inferior occipital gyrus, precuneus, and cingulate cortex (P < 0.001, corrected by AlphaSim). CONCLUSIONS: Cerebral functional alterations occur immediately after RT. This study may provide an explanation for the cognitive deficits in the morphologically normal-appearing brains of NPC patients after RT and may contribute to the understanding of the complex mechanism of RT.

12.
Phys Chem Chem Phys ; 21(20): 10693-10705, 2019 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-31086861

RESUMO

The reactions of cycloaddition, intramolecular H-shift and concerted elimination of alkenyl peroxy radicals are three kinds of important reactions in the low temperature combustion of alkenes. In this study, the cycloaddition reactions are divided into classes considering endo-cycloaddition, exo-cycloaddition and the size of the transition states; the intramolecular H-shift reactions are divided into classes depending upon the ring size of the transition states and the type of C-H bonds from which the hydrogen atom is transferred; the concerted elimination reactions are divided into classes according to the type of H-CßCαOO bond that is broken. All geometry optimizations are performed at the B3LYP/6-31G(2df,p) level. With the electronic structure calculations being performed using the composite Gaussian-4 (G4) method, high pressure limit rate constants and pressure-dependent rate constants at pressures varying from 0.01 to 100 atm are calculated by using canonical transition state theory and the Rice-Ramsberger-Kassel-Marcus/master equation method, respectively. All rate constants are given in the form of the modified Arrhenius expression. The high pressure limit rate rules and the pressure-dependent rate rules are derived by averaging the rate constants of a representative set of reactions in each class. The results show that the rate rules for these three classes of reactions have a large uncertainty and the impact of the pressure on the rate constants increases as temperature increases.

13.
J Biophotonics ; 12(9): e201800422, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31008547

RESUMO

The three-dimensional (3D) mechanical properties characterization of tissue is essential for physiological and pathological studies, as biological tissue is mostly heterogeneous and anisotropic. A digital volume correlation (DVC)-based 3D optical coherence elastography (OCE) method is developed to measure the 3D displacement and strain tensors. The DVC algorithm includes a zero-mean normalized cross-correlation criterion-based coarse search regime, an inverse compositional Gauss-Newton fine search algorithm and a local ternary quadratic polynomial fitting strain calculation method. A 3D optical coherence tomography (OCT) scanning protocol is proposed through theoretical analysis and experimental verification. Measurement errors of the DVC-based 3D OCE method are evaluated to be less than 2.0 µm for displacements and 0.30% for strains by rigid body motion experiments. The 3D displacements and strains of a phantom and a specimen of chicken breast tissue under compression are measured. Results of the phantom show a good agreement with theoretical analysis and tensile testing. The strains of the chicken breast tissue indicate anisotropic biomechanical properties. This study provides an effective method for 3D biomechanical property studies of soft tissue and improves the development of 3D OCE techniques.

14.
PLoS Genet ; 15(2): e1007888, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30707694

RESUMO

Brain metastasis (BM) is a major complication of lung adenocarcinoma (LAD). An investigation of the pathogenic mechanisms of BM, as well as the identification of appropriate molecular markers, is necessary. The aim of this study was to determine the expression patterns of microRNAs (miRNAs) in LAD with BM, and to investigate the biological role of these miRNAs during tumorigenesis. miRNA array profiles were used to identify BM-associated miRNAs. These miRNAs were independently validated in 155 LAD patients. Several in vivo and in vitro assays were performed to verify the effects of miRNAs on BM. We identified six miRNAs differentially expressed in patients with BM as compared to patients with BM. Of these, miR-4270 and miR-423-3p were further investigated. miR-4270 and miR-423-3p directly targeted MMP19 and P21, respectively, to influence cell viability, migration, and colony formation in vitro. miR-4270 downregulation and miR-423-3p upregulation was associated with an increased risk of BM in LAD patients. Thus, our results suggested that miR-4270 and miR-423-3p might play an important role in BM pathogenesis in LAD patients, and that these miRNAs might be useful prognostic and clinical treatment targets.


Assuntos
Adenocarcinoma de Pulmão/genética , Neoplasias Encefálicas/genética , Neoplasias Pulmonares/genética , MicroRNAs/genética , Adenocarcinoma de Pulmão/patologia , Biomarcadores Tumorais/genética , Neoplasias Encefálicas/patologia , Carcinogênese/genética , Carcinogênese/patologia , Movimento Celular/genética , Proliferação de Células/genética , Sobrevivência Celular/genética , Regulação para Baixo/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Regulação para Cima/genética
15.
J Phys Chem A ; 123(4): 742-749, 2019 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-30615442

RESUMO

Nitramine compounds are typical high-energy-density materials (HEDMs) and are widely used as explosives because of their superior explosive performance over conventional energetic materials. In this work, the thermal properties of 1-nitropiperidine (NPIP), 1,4-dinitropiperazine (DNP), and 1,3,5-trinitro-1,3,5-triazinane (RDX) were investigated from quantum mechanics (QM) and reactive force field (ReaxFF) molecular dynamics simulations. We found that the bond dissociation energy of the N-NO2 bond, heat of formation, released energy, produced fragments, and oxygen balance are closely related to the incremental nitramine group. The nitramine group has a significant effect on the energetic performance of these nitramine compounds. In addition, the increase of the nitramine group will improve thermal decomposition activity, promote the generation of small molecules, and restrain the formation of carbon clusters. We hope that this work can shed new light on the design of energetic materials.

16.
Med Dosim ; 44(4): e13-e18, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30529304

RESUMO

The present study aimed to compare 4 techniques in the planning of locoregional irradiation including internal mammary nodal region for left-sided breast cancer. Ten patients with left-sided breast cancer undergoing breast conservation surgery were enrolled. For each patient, 4 treatment plans were performed: a helical tomotherapy (HT) plan, a volumetric modulated arc therapy (VMAT) plan, a static intensity modulated radiation therapy (IMRT) plan, and a hybrid IMRT plan, designed to encompass the whole breast, internal mammary, and supraclavicular nodal regions. The prescribed dose of radiation was 50 Gy in 25 fractions. The dosimetric parameters of the target and organs at risk, as well as the dose delivery time, were evaluated and compared using an independent-samples t-test. The HT and VMAT plans had the best conformity and homogeneity. For the HT, VMAT, IMRT, and hybrid IMRT plans, the mean conformity index (CI) and homogeneity index (HI) were 0.83, 0.82, 0.8, and 0.77 (p < 0.001); and 1.07, 1.11, 1.14, and 1.14 (p < 0.001), respectively. The corresponding V55 values were 0.3%, 11.4%, 27.02%, and 23.29% (p < 0.001). The Dmean and V20 of the left lung obtained using the HT plan were significantly lower than those of VMAT, IMRT, and hybrid IMRT plans (p = 0.002, p = 0.004). There were no significant differences in D max of LAD descending coronary artery, or the Dmean of the heart among the 4 types of plans. The HT and VMAT plans had a lower dose to other organ at risk (OARs) compared with the IMRT and hybrid IMRT plans. The mean delivery times were 1042 ± 33 seconds, 136 ± 12 seconds, 450 ± 65 seconds, and 451 ± 70 seconds for the HT, VMAT, IMRT, and hybrid IMRT plans, respectively (p < 0.001). For whole breast plus supraclavicular and internal mammary nodal irradiation in left-sided breast cancer, the VMAT technique is recommended considering both the dose distribution and the delivery time. Under circumstances in which dose distribution is a priority, the HT technique is a valid option.

17.
Sensors (Basel) ; 18(11)2018 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-30445761

RESUMO

Because of multiple manufacturing phases or operating conditions, a great many industrial processes work with multiple modes. In addition, it is inevitable that some measurements of industrial variables obtained through hardware sensors are incorrectly observed, recorded or imported into databases, resulting in the dataset available for statistic analysis being contaminated by outliers. Unfortunately, these outliers are difficult to recognize and remove completely. These process characteristics and dataset imperfections impose challenges on developing high-accuracy soft sensors. To resolve this problem, the Student's-t mixture regression (SMR) is proposed to develop a robust soft sensor for multimode industrial processes. In the SMR, for each mixing component, the Student's-t distribution is used instead of the Gaussian distribution to model secondary variables, and the functional relationship between secondary and primary variables is explicitly considered. Based on the model structure of the SMR, a computationally efficient parameter-learning algorithm is also developed for SMR. Results conducted on two cases including a numerical example and a real-life industrial process demonstrate the effectiveness and feasibility of the proposed approach.

18.
J Magn Reson Imaging ; 2018 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-30475438

RESUMO

BACKGROUND: Differential diagnosis of the mucosa-associated lymphoid tissue lymphoma (MALToma) and tumor-like benign lymphoepithelial lesion (BLEL) in the parotid gland is difficult. PURPOSE: To distinguish MALToma and BLEL with multimodality MRI including hydrogenproton magnetic resonance spectroscopy (1 H-MRS), diffusion-weighted imaging (DWI-MR), and dynamic contrast-enhanced (DCE-MR), and evaluate each sequence. STUDY TYPE: Retrospective. POPULATION: Twenty-five patients with parotid tumor-like BLEL and 20 with parotid MALToma. FIELD STRENGTH/SEQUENCE: 1.5-T/T1 WI, T2 WI, single-voxel 1 H-MRS, DWI-MR, and DCE-MR. ASSESSMENT: All MR images were interpreted and agreed upon by two radiologists who were blinded to clinical information and histopathologic results. The imaging diagnoses were then compared to the histopathologic results. STATISTICAL TESTS: Youden index was used to determine the optimized threshold value. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficiency of different functional (f)MRI methods. RESULTS: Fisher's exact test indicated a significant difference between the 1 H-MRS images of the two lesions (P < 0.001). The sensitivity, specificity, and accuracy of positive choline (Cho) peak in 1 H-MRS of parotid MALToma were 80%, 76%, and 77.7%, respectively. The mean apparent diffusion coefficient (ADC) was 0.992 × 10-3 mm2 /s in patients with parotid tumor-like BLEL and 0.634 × 10-3 mm2 /s in patients with parotid MALToma, and the difference was statistically significant (t-test, P < 0.001). Choosing the Youden index as 0.669 × 10-3 mm2 /s, the sensitivity, specificity, and accuracy of the assay were 78.9%, 95.8%, and 88.4%, respectively. Assuming that time-intensity curve (TIC) type I indicated parotid MALToma (positive), and type II and type III indicated parotid tumor-like BLEL (negative), the sensitivity, specificity, and accuracy of time-to-peak (TTP) and initial slope of increase (ISI) in diagnosing MALToma were 94.1%, 95.2%, and 94.7%, respectively. Combining methods of TTP, ADC, and Cho peak reached the highest AUC (1.000). DATA CONCLUSION: Combined use 1 H-MRS, DWI-MR, and DCE-MR increased the accuracy of the differential diagnosis between these lesions to 100%. Cho peak in 1 H-MRS, ADC less than 0.669 × 10-3 mm2 /s, TIC type I together indicated parotid MALToma. LEVEL OF EVIDENCE: 3 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2018.

19.
Curr Gene Ther ; 2018 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-30444200

RESUMO

Recent studies of CD19-specific chimeric antigen receptor (CAR)-modified T cells (CARTs) have demonstrated unprecedented successes in treating refractory and relapsed B cell malignancies. The key to the latest CART therapy advances can be attributed to the improved co-stimulatory signals in the CAR design. Here we established several novel CARs by incorporating T cell signaling domains of CD28 in conjunction with intracellular signaling motif of 4-1BB, CD27, OX40, ICOS, and IL-15Rα. These novel CARs were functionally assessed based on a simple target cell killing assay. The results showed that the CD28/IL-15Rα co-signaling (153z) CAR demonstrated the fastest T cell expansion potential and cytotoxic activities. IL-15 is a key cytokine that mediates immune effector activities. The 153z CARTs maintained prolonged killing activities after repetitive rounds of target cell engagement. Consistent with the enhanced target killing function, the 153z CARTs produced increased amount of effector cytokines including IFN-, TNF and IL-2 upon interaction with the target cells. In a follow-up clinical study, an acute lymphoblastic leukemia patient, who had experienced repetitively relapsed central nervous system leukemia (CNSL) and failed all conventional therapies, was enrolled to receive the CD19-specific 153z CART treatment. The patient achieved complete remission after the 153z CART cell infusion. The translational outcome supports further investigation into the safety and enhanced therapeutic efficacy of the IL-15Rα-modified CART cells in cancer patients.

20.
Int Immunol ; 2018 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-30462215

RESUMO

The induction of a dominant Th2-type response is the main cause of harmful inflammation in respiratory syncytial virus (RSV) vaccines trials. A balanced Th1 versus Th2 immune response is needed for a safe and effective RSV vaccine. In this study, we evaluated the potential of a recombinant protein SBP-FG as vaccine candidate with main focus on shifting the harmful Th2 response to Th1 response. SBP-FG consists of epitopes from RSV fusion (F) and attachment (G) proteins conjugated to the N-terminal of HBsAg-binding protein (SBP). SBP-FG induced significantly stronger immune responses assessed at the level of total IgG, IgA and neutralizing antibodies as compared to formalin inactivated-RSV (FI-RSV) and live RSV. Analysis of IgG isotypes, lungs cytokines and T helper cells showed that SBP-FG induced dominant Th1-type response. Further, SBP-FG immunized mice showed significantly reduced lungs eosinophilia, reduced viral replication in lungs after challenge infection and provided protection against RSV infection. These results suggest that SBP-FG can be developed into a safe and effective vaccine against RSV. However, more studies are required to further evaluate SBP-FG as a potent vaccine candidate against RSV.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA