Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 65
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Medicine (Baltimore) ; 99(6): e19004, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32028411

RESUMO

BACKGROUND: Endoscopic tonsillectomy is associated with postoperative pain. Postoperative pain management remains to be improved in children. We aimed to investigate oxycodone preemptive analgesia in children undergoing endoscopic plasma total adenotonsillectomy. METHODS: 166 children with adenotonsillar hypertrophy were recruited at Wuhan Children's Hospital between 08/2016 and 03/2017. They were randomly assigned to receive SPOA (postoperative sufentanil), SPEA+SPOA (preemptive sufentanil and postoperative sufentanil), and OPEA+SPOA (preemptive oxycodone and postoperative sufentanil). The primary endpoint was serum c-fos levels. The secondary endpoints were the response entropy (RE) value, Pediatric Anesthesia Emergence Delirium (PAED) score, FLACC score, and adverse events. RESULTS: c-fos mRNA levels were increased significantly after surgery in the SPOA and SPEA+SPOA groups (P < .05). Postoperatively, c-fos mRNA levels were higher in the SPOA group compared with the OPEA+SPOA group (P = .044). The RE values increased in all groups after surgery (P < .05). At extubation, RE values were higher in the SPOA group compared with the SPEA+SPOA and OPEA+SPOA groups (P < .05). The PAED scores were higher in the SPOA group compared with the OPEA+SPOA group (P = .045). In the SPOA group, the FLACC scores were decreased at 24 h after surgery vs 4 hours (P = .044). Prediction probability (Pk) values indicated that RE and c-fos mRNA levels were quantitative predictors for early postoperative stress reaction after surgery. CONCLUSIONS: The subanalgesic dose of oxycodone (0.1 mg/kg) as preemptive analgesia could improve pain after endoscopic plasma total adenotonsillectomy in children.

2.
Arthritis Res Ther ; 21(1): 282, 2019 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-31831053

RESUMO

BACKGROUND: 18F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) is a promising tool for diagnosing relapsing polychondritis (RP). However, its usefulness in assessing RP with airway involvement is unknown. OBJECTIVE: This study aimed to further evaluate and confirm the potency of 18F-FDG PET/CT in diagnosing RP with airway involvement and monitoring response to steroid-based therapy. METHODS: A total of 30 patients from a dedicated respiratory centre, diagnosed with RP in accordance with McAdam, Damiani or Levine criteria, were included in this study. All patients underwent baseline 18F-FDG PET/CT, and 10 patients underwent second scans after 2.5-15 months of steroid-based therapy. Visual scores (VS) and maximal standard uptake values (SUVmax) were analysed. RESULTS: In the initial scan, 83.3% (25/30) of patients were found to have FDG uptake in more than one cartilage. The median VS and SUVmax in the cartilages were 3 (range, 1-3) and 3.8 (range, 1.9-17.9), respectively. Positive rates for PET/CT-guided biopsy in nasal, auricular, and tracheal/bronchial cartilages were 100% (5/5), 88.9% (8/9), and 10.5% (2/19), respectively, but the positive biopsy rate in the auricular cartilage was 92.3% (12/13) even without PET/CT assessment. Based on biopsy-proven sites, the sensitivity of PET/CT was 55.6%, and the specificity was 5.3%. Compared with the baseline scan, the second scan showed much lower median VS (2 vs 3, respectively; p < 0.0001) and SUVmax (2.9 vs 3.8, respectively; p < 0.001). Of 10 patients who underwent second PET/CT, 8 had complete therapeutic response, while 2 had partial response. CONCLUSION: 18F-FDG PET/CT assists in identifying multiple cartilage involvement in RP, but it seems neither a sensitive nor specific modality in diagnosing RP with airway involvement. Moreover, PET/CT has limited utility in locating biopsy sites and monitoring therapeutic response to corticosteroids.

3.
Front Physiol ; 10: 1334, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31736772

RESUMO

The electroneutral Na+/HCO3 - cotransporter NBCn2 (SLC4A10) of solute carrier family 4 (SLC4) plays important physiological and pathological roles in the body. Our previous study showed that NBCn2 is expressed on the protein level in the small intestine of rat. Here, by reverse-transcription polymerase chain reaction (PCR), we identified a novel full-length NBCn2 variant, i.e., NBCn2-K, from rat small intestine. By pHi measurement with Xenopus oocytes, the activity of NBCn2-K is not significantly different from NBCn2-G. By western blotting, NBCn2 and the Na+/H+ exchanger NHE3 (SLC9A3) are predominantly expressed in the jejunum of rat small intestine. By immunofluorescence, NBCn2 and NHE3 are localized at the apical domain of the jejunum. NaCl overload decreases the expression of NBCn2 by 56% and that of NHE3 by 40% in the small intestine. We propose that NBCn2 is involved in the transepithelial NaCl absorption in the small intestine, and that the down-regulation of NBCn2 by NaCl represents an adaptive response to high salt intake in rat.

4.
J Control Release ; 315: 97-113, 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31644938

RESUMO

Polymeric microneedle (MN) systems are interesting transdermal drug delivery systems because of their controlled drug delivery, tunable properties, and ease of patient self-administration. They are biocompatible and can easily and painlessly penetrate the stratum corneum, delivering their contents into the dermis where they can be adsorbed into systemic circulation. Polymeric MNs can facilitate appropriate therapeutic dosing by controlling the release kinetics of pre-loaded drugs, targeting specific tissues, or responding to changing physiological conditions. This can be accomplished by modifying the degradation and swelling profiles of the host polymer and the diffusion profiles of the encapsulated drugs. In this review various mechanisms of controlled drug delivery using polymeric MNs, including new strategies, applications, and their future outlook are summarized and evaluated.

5.
Mol Carcinog ; 58(12): 2207-2217, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31544294

RESUMO

Recent evidence indicates that long noncoding RNA colon cancer-associated transcript-1 (lncRNA CCAT1) is abundantly expressed in esophageal cancer and is closely related to the occurrence, development, invasion, metastasis, and drug resistance of this disease. However, the role and molecular mechanisms of CCAT1 in the cell proliferation and chemoresistance of esophageal cancer are largely unknown. The correlation between CCAT1 expression and drug resistance to cisplatin (CDDP) in esophageal squamous cell carcinoma (ESCC) cells was analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and quantitative real-time polymerase chain reaction (qRT-PCR) assays. CCAT1 knockdown and miR-143 overexpression or inhibition were used to verify the effects on proliferation and drug resistance via MTT, western blotting, flow cytometry, and immunofluorescence assays. qRT-PCR and western blotting were applied to detect the potential regulatory relationship among CCAT1, miR-143, PLK1, and BUBR1. A xenograft tumor assay was performed to validate the role of CCAT1 in vivo. The expression of CCAT1 was positively correlated with drug resistance in several ESCC cell lines. CCAT1 knockdown and miR-143 overexpression inhibited cell proliferation and CDDP drug resistance. Moreover, the downstream target of CCAT1 was found to be miR-143, which can regulate the expression of PLK1 and BUBR1. In vivo assays showed that CCAT1 knockdown suppressed tumor growth and enhanced the sensitivity of tumors to CDDP in nude mice. Taken together, we discovered a novel mechanism by which CCAT1 promotes cell proliferation and enhances drug resistance by regulating the miR-143/PLK1/BUBR1 signaling axis both in vitro and in vivo. Our findings further suggest that lncRNA CCAT1 may be a potential therapeutic target for overcoming chemoresistance in esophageal cancer.

6.
BMC Infect Dis ; 19(1): 576, 2019 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-31272486

RESUMO

BACKGROUND: Pleural parasitic infestation (PPI) is a disease prevalent in certain parts of the world. It is frequently misdiagnosed due to its lack of standardized diagnostic criteria. The purpose of this study was to evaluate the clinical characteristics of PPI patients and develop a practical diagnostic approach for PPI. METHODS: A retrospective study was conducted by reviewing the medical records of 11 patients with PPI. A practical diagnostic approach was proposed based on the unique laboratory findings. RESULTS: All patients demonstrated respiratory symptoms, including shortness of breath, cough, fever, chest pain, excessive sputum and hemoptysis. Leukocytosis (> 10,000/µL) and eosinophilia (> 500/µL) of peripheral blood were present in 45.5 and 36.4% patients, respectively. The mean concentrations of pleural effusion lactate dehydrogenase (LDH), adenosine deaminase (ADA), protein and carcinoembryonic antigen (CEA) were 338.2 U/L (range, 61-667 U/L), 11.6 U/L (range, 0.1-28.2 U/L), 43.7 g/dL (range, 21.9-88.1 g/dL), and 1.84 mg/mL (range, 0.28-4.8 mg/mL), respectively. The mean percentage of eosinophils in the pleural effusion was 19.5% (10.5-41%). Blood test was positive for parasite-specific IgG antibody in 9 patients, including 4 for Paragonimus westermani, 3 for Taenia solium, 1 for Clonorchis sinensis and 1 for Echinococcus granulosus. Eggs of Clonorchis sinensis were detected in the stool of two patients. Sparganum was found in the pleural effusion of one patient. Respiratory symptoms and abnormal appearances in pulmonary radiographic examination were disappeared in all patients who received anti-parasitic treatment. CONCLUSIONS: In patients with unexplained pleural effusion, parasite-specific IgG antibody tests should be performed when pleural fluid testing shows eosinophilic pleural effusion. It is preferable to consider the diagnosis of PPI in clinical practice when serum parasite-specific IgG antibody test is positive.


Assuntos
Imunoglobulina G/análise , Doenças Parasitárias/diagnóstico , Idoso , Dor no Peito , Tosse , Eosinófilos/patologia , Feminino , Febre , Hemoptise , Humanos , Masculino , Pessoa de Meia-Idade , Doenças Parasitárias/parasitologia , Doenças Parasitárias/patologia , Derrame Pleural/metabolismo , Derrame Pleural/parasitologia , Estudos Retrospectivos , Escarro
7.
Food Funct ; 10(7): 3890-3897, 2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31187836

RESUMO

Gelatin was extracted from the swim bladder of Amur sturgeon with hot water at 50 °C with acceptable yield (76.54%) and it showed type I collagen features. The degree of hydrolysis reached 70.42%, and 26.55% of collagen peptides (380.76 Da) survived, after simulating digestion, absorption and peripheral blood circulation in vitro. In situ single-pass intestinal perfusion showed that the AMW of the collagen peptides was about 1100 Da in the intestine and they were mainly absorbed in the jejunum (61.11%). A 12-month feeding experiment using rats demonstrated that gelatin improved the histological structure, increased the thickness of the dermis (18.45%) and the density of collagen fibers (22.17%), and decreased the ratio of type III to type I collagen (43.44%) of chronologically aged skin at 3.85 g per kg bw per d. Moreover, the antioxidant enzyme activities in skin were significantly enhanced, while the malondialdehyde content was reduced by 31.99%. These results provided new insights into the development of new nutraceuticals and functional foods for the anti-skin-aging effect.


Assuntos
Proteínas de Peixes/farmacologia , Peixes , Gelatina/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Bexiga Urinária/química , Administração Oral , Animais , Colágeno/química , Colágeno/isolamento & purificação , Colágeno Tipo I , Colágeno Tipo III , Digestão , Feminino , Proteínas de Peixes/química , Gelatina/química , Gelatina/isolamento & purificação , Modelos Animais , Ratos , Pele/efeitos dos fármacos , Pele/patologia
8.
Thorac Cancer ; 10(4): 593-600, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30854808

RESUMO

BACKGROUND: To date, few studies have evaluated the impact of lobectomy versus sublobar resection for early small cell lung cancer (SCLC). We investigated the survival rates of patients with pathological stage T1-2N0M0 SCLC who underwent lobectomy or sublobar resection. METHODS: We identified 548 SCLC patients in the Surveillance, Epidemiology, and End Results database who underwent lobectomy or sublobar resection. Propensity score matching (PSM) and Cox regression analysis were used to adjust for baseline characteristics. RESULTS: The three-year overall survival (OS) of patients treated with lobectomy (n = 376, 60%) was significantly higher than those treated with sublobar resection (n = 172, 38%). PSM and Cox multivariable analysis further confirmed this result (hazard ratio [HR] 0.543, 95% confidence interval [CI] 0.421-0.680; P < 0.001). The three-year OS of patients treated with segmentectomy (n = 24, 54%) and wedge resection (n = 148, 36%) was not significantly different (HR 0.639, 95% CI 0.393-1.039; P = 0.071). Based on PSM analysis, segmentectomy conferred a superior survival advantage to patients relative to wedge resection (HR 0.466, 95% CI 0.221-0.979; P = 0.040). CONCLUSION: Lobectomy correlated with superior survival. For patients in which lobectomy is unsuitable, prognosis following segmentectomy appears to be better than after wedge resection.

9.
Molecules ; 24(4)2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30781435

RESUMO

This study investigated the effects of α-tocopherol (α-TOH) on the physicochemical properties of sturgeon surimi during 16-week storage at -18 °C. An aliquot of 0.1% (w/w) of α-TOH was added into the surimi and subjected to frozen storage, and 8% of a conventional cryoprotectant (4% sorbitol and 4% sucrose, w/w) was used as a positive control. Based on total viable count, pH and whiteness, α-TOH exhibited a better protection for frozen sturgeon surimi than cryoprotectant during frozen storage. According to soluble protein content, carbonyl content, total sulfhydryl content, and surface hydrophobicity, α-TOH and cryoprotectant showed the same effects on retarding changes of proteins. The results of breaking force, deformation, gel strength, water-holding capacity and microstructure of sturgeon surimi indicated that the gel properties of frozen sturgeon surimi were retained by α-TOH. Our results suggest that α-TOH is an attractive candidate to maintain the quality of sturgeon surimi during frozen storage.


Assuntos
Crioprotetores/farmacologia , Peixes/metabolismo , Congelamento , alfa-Tocoferol/farmacologia , Animais , Produtos Pesqueiros/análise , Produtos Pesqueiros/microbiologia , Conservação de Alimentos/métodos , Armazenamento de Alimentos/métodos , Compostos de Sulfidrila/metabolismo
10.
J Environ Manage ; 231: 1082-1090, 2019 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-30602232

RESUMO

Transpiration plays a critical role in the water cycle of terrestrial ecosystems, especially for arid ecosystems in which water availability is typically the main constraint for plant growth. Although remote sensing has provided insights into transpiration across different temporal and spatial scales, its potential has not been fully exploited. This is due to a lack of synchronous observations of fluxes and reflectance. Only a few models have attempted to incorporate both radiative transfer and physiological processes. In this study, we calibrated the newly developed Soil, Canopy Observation, Photochemistry and Energy fluxes (SCOPE) model to trace synchronous fluxes of water, energy and reflectance, and thus, their interplays, in a typical arid ecosystem dominated by Haloxylon ammodendron based on long-term continuous field measurements. An initial global sensitivity analysis is conducted to identify parameters that have the greatest impact on model output before subsequent calibration with field data. The resulting calibrated model gives insight into the interplay between reflectance, energy and water fluxes in an arid ecosystem. The calibrated model is thus a useful tool to understand land surface fluxes and radiative transfer processes theoretically, from which additional reflectance information can be exploited to trace the physiological status of ecosystems.


Assuntos
Ecossistema , Solo , Fotoquímica , Água
11.
Brief Bioinform ; 20(1): 102-109, 2019 01 18.
Artigo em Inglês | MEDLINE | ID: mdl-28968662

RESUMO

Adenosine-to-inosine (A-to-I) editing by adenosine deaminase acting on the RNA (ADAR) proteins is one of the most frequent modifications during post- and co-transcription. To facilitate the assignment of biological functions to specific editing sites, we designed an automatic online platform to annotate A-to-I RNA editing sites in pre-mRNA splicing signals, microRNAs (miRNAs) and miRNA target untranslated regions (3' UTRs) from human (Homo sapiens) high-throughput sequencing data and predict their effects based on large-scale bioinformatic analysis. After analysing plenty of previously reported RNA editing events and human normal tissues RNA high-seq data, >60 000 potentially effective RNA editing events on functional genes were found. The RNA Editing Plus platform is available for free at https://www.rnaeditplus.org/, and we believe our platform governing multiple optimized methods will improve further studies of A-to-I-induced editing post-transcriptional regulation.


Assuntos
Adenosina Desaminase/metabolismo , Edição de RNA , Proteínas de Ligação a RNA/metabolismo , Software , Regiões 3' não Traduzidas , Adenosina/genética , Adenosina/metabolismo , Processamento Alternativo/genética , Sequência de Bases , Biologia Computacional , Bases de Dados de Ácidos Nucleicos/estatística & dados numéricos , Feminino , Ontologia Genética , Humanos , Inosina/genética , Inosina/metabolismo , Masculino , MicroRNAs/genética , MicroRNAs/metabolismo , Mutação de Sentido Incorreto , Edição de RNA/genética , Precursores de RNA/genética , Precursores de RNA/metabolismo , Análise de Sequência de RNA/estatística & dados numéricos , Máquina de Vetores de Suporte , Distribuição Tecidual
12.
Environ Monit Assess ; 191(1): 13, 2018 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-30539375

RESUMO

Transpiration plays a key role in water and energy fluxes at various scales. While in recent remote sensing offers a fast and convenient method for tracing transpiration at multiple scales, the approach is mostly indirect and relies on energy balance. Although several hyperspectral indices have been reported to show potentials for tracing transpiration directly, both at leaf and canopy scales, they remain in pioneer stages and need extensive validations. In this study, we used the Soil, Canopy Observation, Photochemistry and Energy fluxes (SCOPE) model calibrated to arid ecosystems in Central Asia, to generate a simulated dataset for validation. Furthermore, new and robust indices have been developed by combining both simulated and in situ measured datasets. Results suggested that the SR(1525, 2150), ND(1425, 2145), and previously reported index of dSR(660,1040) have significant relationships with both simulated and in situ measured transpiration. Further analyses revealed that the ND(1425,2145) shows consistent performance, even with different methodologies of combining simulation and field-measured datasets. Statistically significant results were obtained in this study, even for a dominant drought-tolerant species in arid land, a place that typically has weak vegetation reflectance under strong background radiation. We foresee the approach being conducted in other regions where vegetation reflectance dominates. This may lead to robust hyperspectral indices being developed for directly tracing transpiration at various scales.


Assuntos
Secas , Ecossistema , Monitoramento Ambiental/métodos , Plantas , Folhas de Planta/química , Transpiração Vegetal , Solo , Água/análise
13.
Scand J Gastroenterol ; 53(12): 1604-1610, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30422724

RESUMO

OBJECTIVES: Histopathological examination is pivotal in diagnosing autoimmune pancreatitis (AIP). The usefulness of EUS-guided fine needle aspiration (EUS-FNA) in diagnosing AIP remains controversial worldwide. The authors conducted this study to evaluate the efficacy of EUS-FNA for AIP diagnosis using a 22-gauge needle. METHODS: Between January 2013 and May 2017, 37 patients had imaging studies suggestive of AIP at Tongji Hospital, and 27 patients of them were enrolled in this study. Tissue specimens acquired through EUS-FNA were analyzed for periductal lymphoplasmacytic infiltrate (LPI), storiform fibrosis (SF), obliterative phlebitis (OP) and immunoglobulin G4 (IgG4)-positive plasma cell counts. Clinical Trials.gov no: TJ-C20121220. RESULTS: LPI and SF were present in 18 (66.67%) and 18 (66.67%) of 27 patients, respectively. Abundant IgG4-positive plasmacyte infiltration >10/high-power field (HPF) was detected in 8 of 27 patients (29.63%). OP and the characteristic findings of idiopathic duct-centric chronic pancreatitis (IDCP) and granulocytic epithelial lesion (GEL) were not detected in this study. According to the International Consensus Diagnostic Criteria (ICDC) for AIP, 5 and 12 of 27 patients were assessed as having level 1 and level 2 histological findings, respectively, suggesting that 17 of 27 patients (62.96%) had lymphoplasmacytic sclerosing pancreatitis (LPSP) based on the ICDC. CONCLUSIONS: In 92.6% of patients, pancreatic tissues with >5 HPFs were obtained by EUS-FNA using a 22-G needle. In 63% of patients, histology was evaluated to be ≥ level 2 according to the ICDC. The study indicates that EUS-FNA with a 22-G needle is valuable in the histopathological diagnosis of AIP.


Assuntos
Doenças Autoimunes/diagnóstico , Biópsia por Agulha Fina/métodos , Endossonografia , Pancreatite/diagnóstico , Adulto , Idoso , Idoso de 80 Anos ou mais , Doenças Autoimunes/patologia , China , Feminino , Humanos , Imunoglobulina G/sangue , Masculino , Pessoa de Meia-Idade , Agulhas , Pancreatite/patologia , Estudos Prospectivos
15.
Environ Sci Pollut Res Int ; 25(31): 31705-31717, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30209767

RESUMO

In this research, the adsorptive removal of diclofenac sodium, one of the representative pharmaceuticals and personal care products, from aqueous solution using Fe3O4@MOF-100(Fe) magnetic microspheres was studied for the first time. The Fe3O4@MOF-100(Fe) microspheres exhibit strong magnetism and stability, which were observed as a core-shell structure. The maximum adsorption capacity of Fe3O4@MOF-100(Fe) for diclofenac sodium can reach 377.36 mg L-1, which was higher than most of the adsorbents reported. The adsorption kinetics follows the pseudo-second-order kinetic equation. And the adsorption equilibrium of DCF can be described with Langmuir isotherm. In the cycle experiment, Fe3O4@MOF-100(Fe) material performed high adsorption efficiency for low-concentration diclofenac sodium solution, and the removal rate can still reach 80% after 5 cycles of adsorption without desorption. The mechanisms including electrostatic interaction, H-bond interaction, and π-π interaction that coexisted in the adsorption processes would be of benefit to enhance the adsorption capacity. The Fe3O4@MOF-100(Fe) magnetic microspheres offer exciting opportunities for further application.


Assuntos
Diclofenaco/química , Microesferas , Poluentes Químicos da Água/química , Adsorção , Concentração de Íons de Hidrogênio , Cinética , Magnetismo , Poluentes Químicos da Água/análise , Purificação da Água
16.
J Thorac Dis ; 10(6): 3244-3252, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30069320

RESUMO

Background: The aim of this study was to retrospectively investigate the diagnostic accuracy of ultrasound-guided pleural cutting needle biopsy (US-guided PCNB) and the potential factors influencing diagnostic yield. Methods: From July 2014 to June 2016, a total of 147 percutaneous US-guided PCNBs in 144 patients were retrospectively reviewed. The final diagnosis was confirmed by histopathological analysis and follow-up. We calculated diagnostic accuracy, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) and divided all cases into group of correct diagnoses (true-positive and true-negative cases) and group of incorrect diagnoses (false-positive, false-negative, and inconclusive cases). Univariate and multivariate logistic regression analyses were performed to analyze the differences of influencing factors (patient, pleura, and biopsy-associated factors) in the between the two groups. Results: Seven patients were excluded because of loss to follow-up. A total of 140 cases were ultimately included (105 males and 35 females). There were 105 cases in the correct diagnosis group, and 35 cases in the incorrect diagnosis group. The overall accuracy of US-PCNB was 75.0% and the sensitivity, specificity, PPV, NPV in malignant diagnosis were 58.1%, 99.0%, 96.2%, and 84.2%, respectively. On univariate analysis, variables affecting diagnostic accuracy of US-PCNB were the pleural thickness (<3 mm in thickness 61.0%, ≥3 mm in thickness 85.2%; P=0.001), morphology (non-nodular pleura 71.4%, nodular pleura 95.2%; P=0.026), and needle size (18 G 69.1%, 16 G 87.0%; P=0.022). Finally multivariate logistic regression demonstrated that pleural thickness [odds ratio (OR): 0.278, P=0.003] and needle size (OR: 0.291, P=0.018) independently predicted diagnostic accuracy. Conclusions: Pleural thickness and the size of the biopsy needle were significantly correlated with the diagnostic yield.

17.
J Chromatogr A ; 1570: 109-115, 2018 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-30098730

RESUMO

This paper describes the development of a novel gas chromatography-mass spectrometry-selected ion monitoring (GC/MS-SIM) method for the determination of trace water contents of organic solvents, using the characteristic m/z 18, m/z 17, and m/z 16 ions of H2O as the qualitative ion and the m/z 18 ion as the quantifier ion. The accuracy and precision of this method were validated. An excellent linear correlation was obtained for trace water contents between 0 and 0.5217 wt%, with a correlation coefficient (R2) of 0.9999, in addition to spike recoveries of 82.6-112.6%, and relative standard deviations (n = 6) of 0.4-7.2%. The limit of detection (S/N = 3) and limit of quantitation (S/N = 10) for the trace water contents of organic solvents were 0.0005% wt% and 0.0016% wt%, respectively.The analytical results confirmed that this method was useful for determining the trace water contents of organic solvents, because it has a low detection limit and wide linear range. It requires only small amounts of the samples and enables sample batch analysis. It is very environmentally friendly and saves reagents.


Assuntos
Cromatografia Gasosa-Espectrometria de Massas/métodos , Compostos Orgânicos/análise , Solventes/química , Água/análise , Limite de Detecção , Compostos Orgânicos/química , Água/química
18.
Oncol Lett ; 15(5): 6991-6999, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29725426

RESUMO

The aim of the present study was to evaluate the efficacy of endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) in diagnosing mediastinal and intra-abdominal lymphadenopathies. A total of 154 patients with mediastinal and intra-abdominal lymphadenopathies were included in this retrospective study between February 2010 and March 2015. Malignancy was suspected in the patients as a result of imaging findings and EUS-FNAs were performed to confirm the diagnoses. EUS and EUS-FNA data, as well as hospital medical records, were reviewed. The accuracy of EUS-FNA was 90.8% for diagnosing malignancy and 85.6% for diagnosing benign lymphadenopathy. In combination with flow cytometry (FCM), the accuracy of EUS-FNA to determine lymphoma was 94.2%. Among the malignant lymphadenopathy cases, 80 were caused by metastasis, 19 by lymphoma and 1 by myeloid leukemia. In the 53 benign cases, EUS-FNA revealed a nonspecific inflammatory condition in 27 patients, tuberculosis in 21 patients and Castleman's disease in 5 patients. The factors revealed to be associated with malignant lymphadenopathy included the sex and age of patients, as well as the location and size of the enlarged lymph node. In particular, celiac axis lymphadenopathy was associated with malignancy (23.0% of cases of malignancy, vs. 3.8% of benign lymphadenopathy). EUS-FNA results additionally suggested that the malignant lymph nodes observed in celiac axis were more likely to result from lymphoma (42.1%; 8/19 cases) than metastasis (18.8%; 15/80 cases; P=0.039). By contrast, malignant lymph nodes observed in the mediastinum were more likely to be caused by metastasis (47.5%; 38/80 cases) than lymphoma (10.5%; 2/19 cases; P=0.004). The results of the present study suggested that EUS-FNA is accurate for differentiating between malignancy and benign lymphadenopathy. Therefore, EUS-FNA in combination with FCM analysis, as a minimally invasive and highly sensitive tool, should be routinely performed for the identification of lymphoma. Additionally, examining the enlarged celiac axis lymph nodes of elderly males, who exhibit an increased risk of malignancy, may be beneficial.

19.
Am J Cancer Res ; 8(2): 302-316, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29511600

RESUMO

In this study, we investigated the role of cancer-associated fibroblasts (CAFs) in hepatocellular carcinoma (HCC) progression. We showed that CAFs secrete high levels of IL-6, which promoted stem cell-like properties in HCC cells by activating Notch signaling through STAT3 Tyr705 phosphorylation. These effects were abolished by Notch1 shRNA knockdown in HCC cells or treatment with an IL-6 neutralizing antibody or the p-STAT3 (Tyr705) inhibitor cryptotanshinone. Xenografted liver tumors were larger in nude mice injected with HCC cells and CAFs than in those receiving HCC cells alone. Moreover, immunohistochemical analysis of liver tissue specimens from 88 HCC patients revealed that high nuclear Notch intracellular domain (nNICD) levels in HCC cells correlated with poor prognosis in patients. These findings suggest that IL-6 secreted by CAFs promotes stem cell-like properties in HCC cells by enhancing STAT3/Notch signaling.

20.
Virology ; 518: 272-283, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29550678

RESUMO

Re-emerging human adenoviruses type 14 (HAdV14) and 55 (HAdV55) represent two highly virulent adenoviruses. The neutralizing antibody (nAb) responses elicited by infection or immunization remain largely unknown. Herein, we generated hexon-chimeric HAdV14 viruses harboring each single or entire hexon hyper-variable-regions (HVR) from HAdV55, and determined the neutralizing epitopes of human and mouse nAbs. In human sera, hexon-targeting nAbs are type-specific and mainly recognize HVR2, 5, and 7. Fiber-targeting nAbs are only detectable in sera cross-neutralizing HAdV14 and HAdV55 and contribute substantially to cross-neutralization. Penton-binding antibodies, however, show no significant neutralizing activities. In mice immunized with HAdV14 or HAdV55, a single immunization mainly elicited hexon-specific nAbs, which recognized HAdV14 HVR1, 2, and 7 and HAdV55 HVR1 and 2, respectively. After a booster immunization, cross-neutralizing fiber-specific nAbs became detectable. These results indicated that hexon elicits type-specific nAbs whereas fiber induces cross-neutralizing nAbs to HAdV14 and HAdV55, which are of significance in vaccine development.


Assuntos
Adenovírus Humanos/imunologia , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/imunologia , Proteínas do Capsídeo/imunologia , Reações Cruzadas , Animais , Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Mapeamento de Epitopos , Humanos , Camundongos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA