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1.
Sensors (Basel) ; 20(7)2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-32218106

RESUMO

The Earth's ionosphere is greatly influenced by geomagnetic activities, especially geomagnetic storms. During a geomagnetic storm, the ionosphere suffers many perturbations, leading to a spatial gradient that are neglected during geomagnetically quiet periods. An ionospheric gradient generates potential hazards for a ground-based argumentation system (GBAS) by enlarging the errors in the delay corrections between ground monitor stations and users. To address this problem, this work investigates the characteristics of the ionospheric gradient under geomagnetic storms. Global Navigation Satellite System (GNSS) observations from the continuously operating reference station (CORS) network were used to analyze the ionospheric gradients during the geomagnetic storm on 8 September 2017. The statistical behavior of the ionospheric gradient was further discussed. Experiments show that strong geomagnetic perturbations lead to large ionospheric gradients, and the gradients also vary with the geomagnetic location.

2.
Cancer Sci ; 111(5): 1739-1749, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32167618

RESUMO

We aimed to verify the expression status and diagnostic significance of isocitrate dehydrogenase 1 (IDH1) in non-small-cell lung cancer (NSCLC), especially during early stages. Serum IDH1 levels were measured by ELISA. A total of 1223 participants (660 patients with NSCLC, 276 healthy controls [HCs], 95 patients with benign pulmonary conditions [BPCs], 135 patients with other cancers [OCs], and 57 samples with interfering factors) were divided into a training cohort and a validation cohort according to 3 testing centers. The IDH1 concentrations in the NSCLC group were obviously higher than those in the control groups (P < .001). Area under the receiver operating characteristic curves (AUCs) for discriminating NSCLC patients from controls (HC, BPC, and OC) were 0.870 and 0.745 (sensitivity, 63.3% and 55.0%; specificity, 86.8% and 86.3%) in the training cohort and validation cohort, respectively. The AUCs for discriminating stage 0-IA lung cancer patients from HCs were 0.907 and 0.788 (sensitivity, 58.6% and 59.1%; specificity, 92.9% and 89.3%) in 2 cohorts, respectively. Isocitrate dehydrogenase 1 showed specificity for NSCLC and had no diagnostic value for other common cancers. Furthermore, IDH1 was significantly reduced in postoperative serum. Isocitrate dehydrogenase 1 shows clinical utility as a serum protein biomarker for the early diagnosis of NSCLC.

3.
Sci Total Environ ; 718: 137329, 2020 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-32105925

RESUMO

Sediment adsorption is one of the main fates of antibiotics in aquatic environments. However, few studies have compared the physicochemical properties of sediments from the same aquatic ecosystem but at different locations and correlated them with antibiotic adsorption efficiency. To predict the adsorption of antibiotics in water-sediment systems more accurately, this study conducted experiments with tetracycline (TC) and oxytetracycline (OTC) in tetracyclines, ciprofloxacin (CIP) in fluoroquinolones, and roxithromycin (ROX) in macrolides. Sediments from different locations in Lake Taihu, China, were collected to determine the adsorption properties of CIP, TC, OTC, and ROX. Moreover, the physicochemical properties of the sediments were measured and the correlation between these properties and antibiotic adsorption were discussed to establish a model for predicting adsorption parameter Kd. The antibiotic adsorption performance of sediments was high in heavily polluted and grassy areas but poor in estuarine areas of the lake, suggesting that adsorption effectiveness was affected by the physicochemical properties of sediments. Based on the established model, the specific surface area, organic carbon, and cation exchange capacity played the most significant roles. The study further determined that the predicted and actual values showed a good linear fitting relationship. Therefore, the model effectively predicted the antibiotic adsorption performance of different sediments and provided recommendations for the environmental trend and risk assessment of antibiotics.

4.
Int J Biochem Cell Biol ; 121: 105696, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32001362

RESUMO

Bovine mammary fibrosis represents a considerable health problem of cows, primarily indicated by lactation failure. Staphylococcus aureus (S. aureus) can cause mammary damage, this multifactorial disease necessitates to identify how and to what extent molecular pathogen defense mechanisms prevent bacterial infections in bovine mammary gland. In this study, we have aimed to determine the transcriptional responses in bovine mammary fibroblasts (BMFBs) induced by S. aureus using bioinformatics analysis to determine whether mRNA expression profile changes between BMFBs activation and quiescence. Established primary BMFBs obtained from healthy Holstein bovine were induced 106 CFU/mL heat-inactivated S. aureus and total RNA was isolated 6 h after treatment. The 574 DEGs were involved in gene ontology (GO) that were immune response, apoptotic process, extracellular region, receptor binding, endopeptidase activity and protein kinase activity et al. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, distinct pathway contained signaling molecules common to various inflammatory and fibrotic pathways were Pathways in cancer, Cytokine-cytokine receptor interaction, PI3K-Akt signaling pathway, TNF signaling pathway, MAPK signaling pathway and Toll-like receptor signaling pathway. The BMFBs was treated with heat-inactivated S. aureus (106 CFU/mL) and also with pharmacological inhibitors of ERK1/2, P38 MAPK and JNK. The MMP-2 activity were examined gelatin zymography, MMP-2, TIMP-1, -2 and PLAU/PAI-1 protein expression were examined in vitro by western blot. The MMP-2 activity was significantly inhibited by simultaneous inhibition of ERK1/2, P38 MAPK and JNK, and MMP-2, TIMP-1,-2 and PLAU/PAI-1 protein expression were significantly decreased by inhibiting ERK1/2, P38 MAPK or JNK. This suggested a crosstalk between the ERK1/2, P38 MAPK or JNK signaling pathways in regulating extracellular matrix metabolism in the BMFBs with S. aureus. Our study complement our initial study on S. aureus-induced responses by fibrosis-associated genes in BMFBs. This may lead to development of novel therapeutic targets to control bovine mammary fibrosis induced by S. aureus.

5.
J Biomed Mater Res A ; 108(6): 1351-1368, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32090432

RESUMO

The prosthetic mesh, which is widely used in tension-free hernioplasty, often result in avascular stiff fibrotic scar or mesh shrinkage, causing chronic pain and infection. Here, we developed an autologous bionic tissue (ABT), which was composed of autologous bone marrow-derived mesenchymal stem cells (MSCs), poly (lactic-co-glycolic acid) (PLGA) porous scaffolds, and extracellular matrix (ECM) produced by MSCs for inguinal hernioplasty. In ABT, MSCs produced a variety of ECM composites, such as structural proteins (insoluble collagen, elastin) that provided mechanical properties, macromolecules (hyaluronic acid, glycosaminoglycan) as water and cytokines reservoir, and cell-engaging proteins (fibronectin, laminin). The above ECM composites reached the highest level in 21 days. ECM degradation related cytokines (MMP-9 and its inhibitor TIMP-1) reached the highest level on the 14th day. ECM increased the mechanical properties, elasticity, and flexibility of PLGA. Compared with the PLGA, ABT greatly inhibited inflammatory factors and promoted anti-inflammatory factors (p < 0.05), and gradually reduced the M1/M2 ratio in vivo (p < 0.05). After implantation, the thickness of tissue regeneration (p < 0.05), the number of capillaries or mature vessels (p < 0.05), the mechanical properties of ABT (p < 0.05) were greater than PLGA. MSCs and ECM could reduce the inflammation caused by PLGA, and prevent PLGA from earlier degradation and facilitate host cellular infiltration, thus ABT could greatly promote tissue regeneration in hernia repairs.

8.
Water Environ Res ; 92(1): 84-93, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31332878

RESUMO

Chromium (Cr(VI)) is highly toxic and carcinogenic. Cr(VI) water pollution has become more and more serious. This article reports on a study in which lotus seed pods (LSP), an agricultural waste product, was used to efficiently remove Cr(VI) from an aqueous solution, and the carbonization product of LSP after the removal of Cr(VI) (CPLSP) can be regarded as a resource containing Cr. Cr(VI) removed by LSP fits a pseudo-second-order model. pH levels greatly influence the amount of Cr removed. The maximum removal of Cr(VI) by LSP in aqueous solution was 153.85 mg/g. The possible removal mechanism is absorption, redox, and reabsorption based upon SEM/EDS, FT-IR, and XPS spectra results. The Cr content of CPLSP was 42.95% by ammonium persulfate oxidation titrimetric method. These results suggest that LSP can be an excellent, low cost, biomaterial for removing and enriching Cr(VI) from an aqueous solution. PRACTITIONER POINTS: Lotus seed pods are an efficient adsorbent for Cr(VI) from aqueous solutions. The Cr removal by lotus seed pods occurs via absorption, redox, and reabsorption. Cr can be captured after the pods are carbonized. Lotus seed pods can be applied to the removal and enrichment of Cr(VI) from waste water.


Assuntos
Lotus , Poluentes Químicos da Água , Purificação da Água , Adsorção , Cromo , Concentração de Íons de Hidrogênio , Cinética , Sementes , Soluções , Espectroscopia de Infravermelho com Transformada de Fourier
9.
J Med Entomol ; 57(2): 631-635, 2020 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-31751462

RESUMO

Theileria spp. are tick-transmitted, intracellular apicomplexan protozoan parasites that infect a wide range of animals and, as such, can cause significant economic losses. The aim of the present study was to detect and analyze apicomplexan parasites from two different ectoparasites that were collected from Xinjiang Uygur Autonomous Region, China. The PCR-based detection of 18S rRNA indicated that Ornithodoros lahorensis specimens from Kashgar, Xinjiang, and Aksu were positive for Theileria spp., as were Melophagus ovinus specimens from Aksu. Meanwhile, phylogenetic analysis, based on the 18S rRNA gene sequences, revealed that the four amplified Theileria sequences could be attributed to T. ovis. To the best of our knowledge, this is the first study to report the detection of T. ovis DNA in M. ovinus and the first molecular identification study to confirm the detection of T. ovis in O. lahorensis in China. Accordingly, the present study extends the known distribution of T. ovis.

10.
Biosci Rep ; 40(1)2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-31868206

RESUMO

A Chinese family with matrilineally inherited obesity was assessed and its clinical, genetic, and molecular profiling was conducted. Obesity was observed in matrilineal relatives (3 out of 7) of a single generation (of 3 alive generations) in this family. On pedigree analysis and sequencing of their mitochondrial DNA (mtDNA), a novel homoplasmic mutation of the mitochondrial tRNACys gene (5802A>G) was identified in these individuals. This mutation correlated with a destabilized conserved base pair in this tRNA anticodon stem. Position 30 is known to be crucial for carrying out effective codon recognition and stability of tRNA. In accordance with the importance of this conserved site, we observed that the predicted structure of tRNACys with the mutation was noticeably remodeled in a molecular dynamics simulation when compared with the isoform of the wild-type. All other 46 mutations observed in the individual's mtDNA were known variants belonging to haplogroup D4. Thus, this is the first report that provides evidence of the association between a mutation in tRNA and an enhanced risk of maternally transmissible obesity, offering more insights into obesity and its underlying nature.

11.
Zhongguo Zhong Yao Za Zhi ; 44(11): 2236-2243, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359648

RESUMO

The single-factor test was used to optimize the high-pressure homogenization method to prepare the phenolic extract nanosuspensions(DBNs). The physicochemical properties of the obtained nanosuspensions were characterized and the cumulative release in vitro was evaluated. The results showed that the drug concentration was 0.5 g·L~(-1), the mass concentrations of PVPK30 and SDS were 0.5 and 0.25 g·L~(-1), respectively, the probe ultrasonic time was 5 min, the homogenization pressure was 900 bar, and the number of homogenization was 2 times. The prepared DBNs had an average particle size of(168.80±0.36) nm, polydispersity index(PDI) of 0.09±0.04, stability index(SI) of 0.85, and DBNs were stable for storage within 30 days. Scanning electron microscopy showed that the particle size of the dragon's blood extract was reduced and the uniformity was improved in the obtained nanosuspensions. X-ray diffraction pattern and differential scanning calorimetry showed that the phenolic extract of dragon's blood was still in an amorphous state after being prepared into nanosuspensions. The results of saturated solubility measurement showed that the solubility of DBNs lyophilized powder reached 6.25 g·L~(-1), while the solubility of DB raw powder was only 28.67 mg·L~(-1). The in vitro dissolution experiments showed that DBNs lyophilized powder accumulated in gastrointestinal fluid for 8 h. The release amount was 90%,the cumulative release of the raw powder in the gastrointestinal fluid for 24 h was less than 1%, and the solubility and dissolution rate of the DBNs lyophilized powder were significantly higher than the DB raw powder. The method is simple in process and convenient in operation, and can successfully prepare uniform and stable nanosuspensions to improve its solubility, and provides a research basis for solving the application limitation of dragon's blood extract.


Assuntos
Nanopartículas , Extratos Vegetais/química , Varredura Diferencial de Calorimetria , Tamanho da Partícula , Solubilidade , Suspensões , Difração de Raios X
12.
Appl Environ Microbiol ; 85(16)2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31175187

RESUMO

Two new modified Bacillus thuringiensis (Bt) proteins, Cry1Da_7 and Cry1B.868, with activity against fall armyworms (FAW), Spodoptera frugiperda (J.E. Smith), were evaluated for their potential to bind new insect receptors compared to proteins currently deployed as plant-incorporated protectants (PIPs) in row crops. Results from resistant insect bioassays, disabled insecticidal protein (DIP) bioassays, and cell-based assays using insect cells expressing individual receptors demonstrate that receptor utilizations of the newly modified Cry1Da_7 and Cry1B.868 proteins are distinct from each other and from those of commercially available Bt proteins such as Cry1F, Cry1A.105, Cry2Ab, and Vip3A. Accordingly, these two proteins target different insect proteins in FAW midgut cells and when pyramided together should provide durability in the field against this economically important pest.IMPORTANCE There is increased concern with the development of resistance to insecticidal proteins currently expressed in crop plants, especially against high-resistance-risk pests such as fall armyworm (FAW), Spodoptera frugiperda, a maize pest that already has developed resistance to Bacillus thuringiensis (Bt) proteins such as Cry1F. Lepidopteran-specific proteins that bind new insect receptors will be critical in managing current Cry1F-resistant FAW and delaying future resistance development. Results from resistant insect assays, disabled insecticidal protein (DIP) bioassays, and cell-based assays using insect cells expressing individual receptors demonstrate that target receptors of the Cry1Da_7 and Cry1B.868 proteins are different from each other and from those of commercially available Bt proteins such as Cry1F, Cry1A.105, Cry2Ab, and Vip3A. Therefore, pyramiding these two new proteins in maize will provide durable control of this economically important pest in production agriculture.

13.
World J Pediatr ; 15(4): 382-389, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31124039

RESUMO

BACKGROUND: There is tremendous increase in obesity worldwide. Many factors including diet, life style, genetic, and epigenetic changes contribute to obesity. The fat mass and obesity-associated (FTO) gene polymorphisms are strongly associated with obesity. It has been reported that FTO single-nucleotide polymorphisms (SNPs) are associated with obesity in European populations; however, it was controversial in Chinese population. The present meta-analysis study was designed to investigate the association between FTO polymorphisms and obesity risk in Chinese population. METHODS: The investigators searched studies using the following databases: PubMed, web of science, and Cochrane Central Register of Controlled Trials. A random-effects model was used to calculate the pooled odds ratios (ORs). The heterogeneity among the studies was measured by I2 value. Subgroup analysis was used to find out the potential factors influencing the heterogeneity. RESULTS: A total of 18 articles including 26 studies were included in the present meta-analysis. Overall, the FTO SNPs were significantly associated with obesity in Chinese population (OR 1.30; 95% CI 1.19-1.42; P < 0.001) under per-allele comparison. The subgroup analysis also showed strong association between four FTO SNPs (rs9939609, rs6499640, rs8050136, and rs1558902) and obesity risk. Furthermore, subgroup analysis stratified by children/adolescent and adult groups showed same trend. CONCLUSION: The present meta-analysis indicated that FTO SNPs are associated with obesity risk in both children/adolescents and adults in Chinese population.

14.
Biochem Biophys Res Commun ; 513(2): 460-464, 2019 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-30967269

RESUMO

Abscisic acid (ABA), one of phytohormones, is induced in response to abiotic stress to mediate plant acclimation to environmental challenge. Key players of the ABA-signaling pathway are the ABA-binding receptors (RCAR/PYR1/PYL), which perceive ABA and then inhibit PP2Cs to activate SnRK2s. Here, we report that a putative receptor-like cytoplasmic kinase (RLCK) in Arabidopsis named CARK6, which is a member of cytosolic ABA receptor kinases. We confirm that CARK6 interacts with ABA receptors, RCAR11-14 in vitro and in vivo. Induced overexpression of CARK6 in Arabidopsis enhances sensitivity to ABA by inhibition of seed germination and root elongation, and promotes the drought resistance. However, loss-of-function seedlings of cark6 are less sensitive to ABA and show reduced drought stress response with respect to water loss and stomatal aperture. In transgenic Arabidopsis complementation lines in the cark6 mutant background, stress responsivity was restored by CARK6. In conclusion, our data provide evidence that CARK6 plays a positive role in ABA signaling in Arabidopsis.

15.
J Am Vet Med Assoc ; 254(5): 595-597, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30779628
16.
Arch Microbiol ; 201(3): 349-355, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30635670

RESUMO

A Gram-stain-negative, aerobic, non-motile, rod-shaped and non-spore-forming bacterium, designated YXT, was isolated from wetland soil. Compared to strain YXT, Pedobacter yonginense HMD1002T had the highest 16S rRNA gene sequence identity (97.8%), and the remaining strains had the identities below 97%. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain YXT grouped with P. yonginense HMD1002T. The values of DNA-DNA hybridization and genomic orthologous average nucleotide identity (orthoANI) between strain YXT and Pedobacter yonginense KCTC 22721T were 58.5% and 82.0%, respectively. The genome size of strain YXT was 5.0 Mb, comprising 4369 predicted genes with a DNA G+C content of 37.3 mol %. Strain YXT had menaquinone-7 as the only respiratory quinone and polar lipids of phosphatidylethanolamine, sphingolipid, aminolipid and three unidentified lipids. The predominant cellular fatty acids (> 10%) of strain YXT were summed feature 3 (iso-C15:0 2-OH and/or C16:1ω7c), iso-C15:0 and iso-C17:0 3-OH. Strain YXT could be distinguished from the other Pedobacter members based on the data of phylogenetic distance, DNA-DNA hybridization, genomic orthoANI, RecA MLSA, core-protein comparison, and hydrolyses of L-arginine, utilization of D/L-lactate, L-alanine, 5-ketonic gluconate and glycogen. Therefore, strain YXT represents a novel species of the genus Pedobacter, for which the name Pedobacter paludis sp. nov. is proposed. The type strain is YXT (= KCTC 62520T = CCTCC AB 2018029T).


Assuntos
Pedobacter , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases/genética , DNA Bacteriano/genética , Ácidos Graxos/análise , Hibridização de Ácido Nucleico/genética , Pedobacter/classificação , Pedobacter/genética , Pedobacter/isolamento & purificação , Fosfatidiletanolaminas/metabolismo , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Solo , Vitamina K 2/análogos & derivados , Vitamina K 2/metabolismo , Áreas Alagadas
17.
Sensors (Basel) ; 19(2)2019 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-30669475

RESUMO

Transfer alignment on a moving base under a complex dynamic environment is one of the toughest challenges in a strapdown inertial navigation system (SINS). With the aim of improving rapidity and accuracy, velocity plus attitude matching is applied in the transfer alignment model. Meanwhile, the error compensation model is established to calibrate and compensate the errors of inertial sensors online. To suppress the filtering divergence during the process of transfer alignment, this paper proposes an improved adaptive compensation H∞ filtering method. The cause of filtering divergence has been analyzed carefully and the corresponding adjustment and optimization have been made in the proposed adaptive compensation H∞ filter. In order to balance accuracy and robustness of the transfer alignment system, the robustness factor of the adaptive compensation H∞ filter can be dynamically adjusted according to the complex external environment. The aerial transfer alignment experiments illustrate that the adaptive compensation H∞ filter can effectively improve the transfer alignment accuracy and the pure inertial navigation accuracy under a complex dynamic environment, which verifies the advantage of the proposed method.

18.
Int J Nanomedicine ; 13: 7997-8012, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30538473

RESUMO

Introduction: Polymeric micelles (PMs) hold promise for improving solubility and oral absorption of poorly soluble drugs. Unfortunately, the oral absorption of PMs is also limited by intestinal epithelium. To improve the oral delivery efficiency of micelles, transporter-mediated micelles could enhance the transport efficiency across the epithelial barrier, and they have attracted more attention. Methods: Peptide transporter 1 (PepT1)-mediated micelles (Val-PMs/Phe-PMs) were designed by grafting valine (or phenylalanine) onto the surface of curcumin (Cur)-loaded-D-α-tocopheryl polyethylene glycol 1000 succinate micelles (TP-PMs). The oral absorption mechanism and oral bioavailability were further investigated in vitro and in vivo. Results: The cellular study showed that Val-PMs/Phe-PMs had a high PepT1 affinity, resulting in a higher drug uptake and transcellular transport than TP-PMs. In rats, Val-PMs/Phe-PMs exhibited higher intestinal accumulation in the apical side of the intestinal epithelium than TP-PMs, promoting drug diffusion across epithelial barrier. The oral bioavailability of Cur was significantly improved by Val-PMs/Phe-PMs, which was about 10.50- and 3.40-fold greater than that of Cur-Sol and TP-PMs, respectively. Conclusion: PepT-1-mediated micelles, using PepT1 as a target on intestinal epithelium, have unique functions with intestine and prove promising for oral delivery of poorly water-soluble drugs.


Assuntos
Neoplasias do Colo/metabolismo , Curcumina/administração & dosagem , Absorção Intestinal/efeitos dos fármacos , Intestinos/fisiologia , Micelas , Transportador 1 de Peptídeos/antagonistas & inibidores , Administração Oral , Animais , Antineoplásicos/administração & dosagem , Antineoplásicos/química , Antineoplásicos/farmacologia , Disponibilidade Biológica , Neoplasias do Colo/tratamento farmacológico , Neoplasias do Colo/patologia , Curcumina/química , Curcumina/farmacologia , Portadores de Fármacos/metabolismo , Humanos , Intestinos/efeitos dos fármacos , Masculino , Transportador 1 de Peptídeos/metabolismo , Polímeros/administração & dosagem , Polímeros/química , Ratos , Ratos Sprague-Dawley , Transcitose , Células Tumorais Cultivadas
19.
Sensors (Basel) ; 18(11)2018 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-30424575

RESUMO

The strapdown gravimetry system uses the combination of an Inertial Measuring Unit (IMU) and a Global Navigation Satellite System (GNSS) to measure the Earth's gravity field. Due to limited accuracies of IMU and GNSS, early strapdown gravimetry systems were more often used in airborne surveys, but less used in marine surveys. We developed a strapdown inertial navigation system (SINS), the Sea-Air Gravimeter-2Marine (SAG-2M), using novel IMU components, whose accuracy was further improved with the application of Precise Point Positioning (PPP) and enhanced algorithm, making it possible to be used in marine gravity survey. The testing results of the SAG-2M were compared to those of the Lacoste and Romberg S-129 gravimeter on the same ship in the South China Sea basin. The cruise lasted for 50 days, during which 134 effective gravity profiles were measured, resulting in 174 crossover points. The results showed that, for the SAG-2M, the root mean square (RMS) crossover points were 1.35 mGal before difference adjustment and 0.69 mGal after difference adjustment; for the S-129 gravimeter, they were 5.62 mGal and 0.95 mGal, correspondingly. In calm sea conditions, the results of the two systems were relatively consistent at all wavelengths. However, in rough sea conditions, since the SAG-2M was not affected by the cross-coupling effect, its data demonstrated less high-frequency jump. A physical platform adopted in SAG-2M can further make the transition data effective when the ship is turning around. Therefore, SAG-2M was able to improve the proportion of valid data and the efficiency of data post-processing for measurements taken during the cruise. The testing results indicate that in terms of accuracy and efficiency in the marine gravity survey, SAG-2M is better than S-129. In addition, as the miniaturization and precision of inertial components are developing continuously, SAG-2M also shows great potential in miniaturization.

20.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 34(9): 806-811, 2018 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-30463652

RESUMO

Objective To investigate the expression of lncRNA SNHG6 in tongue cancer and Tca1183 cells, and its effect on the biological characteristics of tongue cancer cells. Methods SNHG6 expression levels in tongue cancer tissue, normal tissue, normal tongue cells and cancer cells were detected by real time quantitative PCR to analyze the correlation between SNHG6 expression and clinicopathological characteristics of tongue cancer. SNHG6 interference plasmid was constructed and transfected into Tca1183 cells. MTT assay was used to detect the viability of Tca1183 cells. Colony formation assay was used to measure the proliferation of Tca1183 cells. The apoptosis of Tca1183 cells was detected by flow cytometry. Western blot analysis was used to evaluate the epithelial mesenchymal transition (EMT) related protein levels of ß-catenin, E-cadherin, N-cadherin and vimentin. Results Compared to normal tissue or normal tongue epithelial cells, SNHG6 expression levels increased significantly in tongue cancer tissue or Tca1183 cells. SNHG6 expression in tongue cancer tissue was not correlated with age and gender, but with tissue differentiation degree, clinical stage and lymph node metastasis. Knockdown of SNHG6 in Tca1183 cells significantly inhibited the cell viability and proliferation and induced apoptosis. Furthermore, knockdown of SNHG6 significantly inhibited the EMT of Tca1183 cells by inducing the ß-catenin and E-cadherin protein expression and inhibiting N-cadherin and vimentin protein expression. Conclusion SNHG6 expression is significantly increased in tongue cancer, and the interference of SNHG6 expression can inhibit the proliferation and EMT of tongue cancer cells.


Assuntos
RNA Longo não Codificante , Neoplasias da Língua , Apoptose , Caderinas , Linhagem Celular Tumoral , Proliferação de Células , Transição Epitelial-Mesenquimal , Humanos
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