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1.
Radiology ; : 211762, 2022 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-35503014

RESUMO

Background The aging brain is typically associated with aberrant interactions of large-scale intrinsic networks. However, the dynamic variation of these networks' coactivation or deactivation across the adult lifespan remains unclear. Purpose To promote the interpretation of dynamic brain network variations underlying the complex aging process by quantifying activation levels and obtaining a clear definition of coactivation patterns (CAPs) with resting-state functional MRI (rsfMRI). Materials and Methods In a retrospective study (October 2010 to September 2013), rsfMRI data from healthy participants in the Cambridge Centre for Ageing and Neuroscience (Cam-CAN) data repository were used to generate CAPs by applying single-volume temporal clustering analysis. Spatial clustering analysis was then performed to capture dynamic coactivation and deactivation within or between primary sensory networks and high-order cognitive networks (including the default mode network [DMN], attentional network [AN], and frontoparietal network [FPN]). Linear relationships between dynamic metrics and age were revealed with Spearman partial correlations. Results A total of 614 participants (mean age, 54 years ± 18 [SD]; 311 women) ranging in age from 18 to 88 years were evaluated. There was a negative correlation of the CAPs (Spearman correlations: r = -0.98, P < .001) with loss of coactivation (partial correlations: r = -0.17, P < .001) and deactivation (partial correlations: r = 0.216, P < .001) with aging. The CAPs, characterized by negative correlation patterns between the DMN and AN, occurred (partial correlations: r = 0.14, P = .003) and dwelled (partial correlations: r = 0.10, P = .04) more with aging. Moreover, the AN and DMN CAP transitioned more to the AN and FPN CAP with aging (partial correlations: r = 0.17, P < .001). Conclusion The dynamics of the healthy aging brain are characterized mainly by more flexibility of the high-order cognitive networks while maintaining primary sensory functions (networks). Online supplemental material is available for this article. © RSNA, 2022 See also the editorial by Holodny in this issue.

2.
Front Neurol ; 13: 843198, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35547389

RESUMO

Objective: To detect fundus changes in patients with cerebral small vessel disease (CSVD) using optical coherence tomography angiography (OCTA) and to investigate the correlations between CSVD and fundus changes. Methods: From January 2019 to January 2020, patients diagnosed with CSVD by magnetic resonance imaging (MRI) were enrolled in our study and received fundus examinations using OCTA. CSVD was defined as white matter hyperintensities, enlarged perivascular spaces, lacunes, or microbleeds on MRI. OCTA parameters included foveal avascular zone areas, retinal nerve fiber layer thickness, and capillary densities of the superficial retinal capillary plexuses, deep retinal capillary plexuses, and the radial peripapillary capillary network of the disc. Univariate and multivariate logistic regression analyses were performed to explore the correlation between CSVD and fundus changes. Results: A total of 115 patients (40% male) were enrolled and analyzed, and the mean age was 65.11 ± 11.23 years. After multivariate logistic regression analysis, the radial peripapillary capillary network density was negatively correlated with severity of deep white matter lesions (OR: 0.909; 95% CI: 0.828-0.998; p = 0.046) and perivascular spaces (OR: 0.881; 95% CI: 0.779-0.995; p = 0.041). Parafoveal vessel densities of the superficial retinal capillary plexuses were independently correlated with lacunes (OR: 0.889; 95% CI: 0.817-0.967; p = 0.006). Conclusion: OCTA parameters were correlated with CSVD, indicating that OCTA is a potential method for CSVD screening.

3.
Front Comput Neurosci ; 16: 822237, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35573265

RESUMO

Functional magnetic resonance imaging (fMRI) is one of the best techniques for precise localization of abnormal brain activity non-invasively. Machine-learning approaches have been widely used in neuroimaging studies; however, few studies have investigated the single-voxel modeling of fMRI data under cognitive tasks. We proposed a hybrid one-dimensional (1D) convolutional neural network (1D-CNN) based on the temporal dynamics of single-voxel fMRI time-series and successfully differentiated two continuous task states, namely, self-initiated (SI) and visually guided (VG) motor tasks. First, 25 activation peaks were identified from the contrast maps of SI and VG tasks in a blocked design. Then, the fMRI time-series of each peak voxel was transformed into a temporal-frequency domain by using continuous wavelet transform across a broader frequency range (0.003-0.313 Hz, with a step of 0.01 Hz). The transformed time-series was inputted into a 1D-CNN model for the binary classification of SI and VG continuous tasks. Compared with the univariate analysis, e.g., amplitude of low-frequency fluctuation (ALFF) at each frequency band, including, wavelet-ALFF, the 1D-CNN model highly outperformed wavelet-ALFF, with more efficient decoding models [46% of 800 models showing area under the curve (AUC) > 0.61] and higher decoding accuracies (94% of the efficient models), especially on the high-frequency bands (>0.1 Hz). Moreover, our results also demonstrated the advantages of wavelet decompositions over the original fMRI series by showing higher decoding performance on all peak voxels. Overall, this study suggests a great potential of single-voxel analysis using 1D-CNN and wavelet transformation of fMRI series with continuous, naturalistic, steady-state task design or resting-state design. It opens new avenues to precise localization of abnormal brain activity and fMRI-guided precision brain stimulation therapy.

4.
Front Oncol ; 12: 817969, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35574341

RESUMO

Background: Intravascular large B-cell lymphoma (IVLBCL) is a rare, aggressive, large B-cell non-Hodgkin's lymphoma. The prognosis of IVLBCL in patients with central nervous system recurrence after first-line chemotherapy treatment is extremely poor. Among immunotherapies, chimeric antigen receptor (CAR) T-cell immunotherapy has been recently found to be a highly effective treatment for B-cell lymphoma, especially for relapsed or refractory diffuse large B-cell lymphoma. However, no guidelines are available that provide a clear consensus regarding the management of patients with relapsed/refractory IVLBCL. Here, we report, for the first time, the use of autologous hematopoietic stem cell transplantation (ASCT) and CAR T-cell therapy in a patient with relapsed/refractory IVLBCL. Case Presentation: A 42-year-old woman was diagnosed with IVLBCL based on liver biopsy and developed central nervous system (CNS) progression. The patient received ASCT combined with murine monoclonal anti-CD19 and anti-CD22 CAR T-cell therapy. She achieved complete remission for 22 months so far with negative minimal residual disease and continues to be followed up. Conclusion: ASCT combined with CAR T-cell therapy was the best choice for treatment of relapsed/refractory IVLBCL, as it allowed the achievement of a lasting complete remission.

5.
Vox Sang ; 2022 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-35560059

RESUMO

BACKGROUND AND OBJECTIVES: To advance blood transfusion safety, the Chinese Haemovigilance Network (CHN) was put into operation in 2018. This report describes the development of the CHN and evaluates its role by analysing reported adverse transfusion reactions (ATRs) from 2018 to 2020. MATERIALS AND METHODS: All data in this study were obtained from the CHN online reporting platform. A timeline of CHN development is presented, and the activities of CHN-enrolled facilities are analysed by year. The reported ATRs were analysed in detail for ATR types, blood components involved and adherence to case definition, severity and imputability criteria. Incidence rates were calculated and compared with international examples. RESULTS: During 2018-2020, a total of 3061 ATRs were reported through the CHN online reporting system. The rate of reported ATRs in all facilities and the 10 highest reporting facilities was 0.7‰ and 1.8‰, respectively. When analysed by year, the incidence rate showed an increasing trend from 2018 to 2020. Allergic (68.2%) and febrile non-haemolytic transfusion reaction (27.1%) were the most common. The vast majority of ATRs (92.0%) were not serious, but serious cases of transfusion-associated circulatory overload, transfusion-associated dyspnoea and hypotensive reaction were common. Most (86.0%) of reported cases were definitely or probably associated with transfusion. CONCLUSION: Under-reporting of ATRs occurs in many Chinese hospitals, but the establishment of CHN has increased ATR recognition and management. More effort will be needed in the future to detect transfusion problems and improve transfusion practice in China.

6.
Neuroinformatics ; 2022 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-35608748

RESUMO

Resting-state magnetic resonance imaging (RS-fMRI) studies indicated that the repetitive transcranial magnetic stimulation (rTMS) exerts antidepression effect through the functional connectivity (FC) of the DLPFC with the subgenual anterior cingulate cortex (sgACC), pregneual ACC (pgACC), or nucleus accumbens (NAc). It is proposed that the FC-guided individualized precise stimulation on the DLPFC would be more effective. The current study systematically investigated the reliability of the RS-fMRI FC location as well as the FC strength with multiple potential factors. We aimed to provide a stable stimulation target for future FC-guided TMS therapy for affective related disorders. Twenty-one subjects under RS-fMRI conditions with the first two times (V1, V2) scanned on a GE 3 T scanner and the third visit (V3) on a Siemens 3 T scanner. Then the FC strength and location reliability were assessed by using intra-class correlation (ICC) and intra-individual distance, respectively. The factors included deep seed ROIs (midline (mid-) sgACC, left pgACC, mid-pgACC, and left NAc), eyes closed (EC) vs eyes open (EO), frequency bands, FC algorithm (Pearson vs Spearman), scanning length (half a session vs whole session), and location method (FC peak vs center of gravity (COG)). The reliability of the voxel-wise FC strength was low to moderate. The intra-individual distances of the COG were 3.8-7.3 mm across all factors, much smaller than that of FC peak (approximately 30 mm). The COG of seed-based FC might be a potential rTMS stimulation target. Anyway, all potential stimulation targets should be tested in future rTMS treatment studies.

7.
Appl Bionics Biomech ; 2022: 7934690, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35535323

RESUMO

In order to compare the microscopic ultrastructure of eutopic endometrium and normal endometrium in patients with endometriosis, to study the specific pathogenesis of endometriosis. In this paper, on the basis of using B-ultrasound technology, several patients with endometriosis were subjected to B-ultrasound to observe the ultrastructure of the eutopic uterine endometrium and compared with the pictures of normal endometrium to carry out the specific analysis between the two ultrastructural comparisons. This study is based on the analysis of B-ultrasound images of patients with endometriosis, compares the difference between their ultrastructure and normal human body, and conducts specific pathological diagnosis and analysis to find out the impact of the endometrium in place. The specific factors of the occurrence of lesions and the corresponding treatment methods are proposed. The experimental results show that the ultrastructure of endometriosis eutopic endometrium is different from that of normal endometrium. The microvilli of secretory cells and the cilia of ciliated cells of the former are abnormally increased and lengthened, and they are superior to B-ultrasound technology. The success rate of the examination is 93.75%, which can play an important role in the specific examination process of patients with endometriosis, as one of the actual indicators of detection. Under the electron microscope, microvilli are tiny finger-like protrusions extending from the cell membrane and the cytoplasm on the free surface of the cell, surrounded by the cell membrane and perpendicular to the cell membrane surface.

8.
HLA ; 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35536653

RESUMO

The novel HLA-B*48:54 allele was identified in an individual from the Sichuan province of China. This article is protected by copyright. All rights reserved.

9.
J Ethnopharmacol ; 294: 115345, 2022 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-35526732

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Rhodiola crenulata (Hook.f. & Thomson) H.Ohba has a long history of clinical application for the prevention and treatment of acute mountain sickness (AMS) in traditional Chinese medicine. However, gaps in knowledge still exist in understanding the underlying mechanisms of Rhodiola crenulata against AMS. AIMS: To address this problem, a comprehensive method was established by combining UHPLC-Q-TOF-MS/MS analysis and network pharmacology. MATERIALS AND METHODS: The ingredients of Rhodiola crenulata were comprehensively analyzed using UHPLC-Q-TOF-MS/MS method. On this basis, a network pharmacology method incorporated target prediction, protein-protein interaction network, gene enrichment analysis and components-targets-pathways network was performed. Finally, the possible mechanisms were verified through molecular docking, in vitro and in vivo experiments. RESULTS: A total of 106 constituents of Rhodiola crenulata were charactered via UHPLC-Q-TOF-MS/MS. The 98 potentially active compounds out of 106 were screened and corresponded to 53 anti-AMS targets. Gene enrichment analysis revealed that hypoxia and inflammation related genes may be the central factors for Rhodiola crenulata to modulate AMS. Molecular docking revealed that TNF, VEGFA and HIF-1α had high affinities to Rhodiola crenulata compounds. Subsequently, Rhodiola crenulata extract was indicated to inhibit the protein expression level of TNF in hypoxia induced H9c2 cells. Lastly, Rhodiola crenulata extract was further verified to ameliorate heart injury and decreased the heart levels of TNF, VEGFA and HIF-1α in acute hypoxia-induced rats. CONCLUSIONS: This study used UHPLC-Q-TOF-MS/MS analysis and a network pharmacology to provide an important reference for revealing the potential mechanism of Rhodiola crenulata in the prevention and treatment of AMS.

10.
Artigo em Inglês | MEDLINE | ID: mdl-35536823

RESUMO

Homography estimation is a basic image alignment method in many applications. It is usually done by extracting and matching sparse feature points, which are error-prone in low-light and low-texture images. On the other hand, previous deep homography approaches use either synthetic images for supervised learning or aerial images for unsupervised learning, both ignoring the importance of handling depth disparities and moving objects in real world applications. To overcome these problems, in this work we propose an unsupervised deep homography method with a new architecture design. In the spirit of the RANSAC procedure in traditional methods, we specifically learn an outlier mask to only select reliable regions for homography estimation. We calculate loss with respect to our learned deep features instead of directly comparing image content as did previously. To achieve the unsupervised training, we also formulate a novel triplet loss customized for our network. We verify our method by conducting comprehensive comparisons on a new dataset that covers a wide range of scenes with varying degrees of difficulties for the task. Experimental results reveal that our method outperforms the state-of-the-art including deep solutions and feature-based solutions.

11.
PLoS One ; 17(5): e0267553, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35507626

RESUMO

A large amount of gas, such as CO, accumulates in a coal mine after an explosion, leading to CO poisoning. In this study, a self-developed platform was used to eliminate CO from coal mines and determine the mass of the rapidly eliminated CO and its concentration in the eliminated gases. Equations were derived to calculate the amount of CO eliminated and the removing rate. The results showed that a rapid removing reagent in the form of nonprecious metal catalysts is useful for removing CO. Removing agents with larger masses facilitated the activation, irrespective of the CO concentration. For removing reagent amounts of 10, 15, 20, 25, and 30 g, the amount of CO eliminated, the removing rate, and the time required to complete catalytic oxidation increased sequentially. The CO removing process could be divided into three stages (I, II, and III) based on the variations in the CO, CO2, and O2 concentrations during CO removing. The removing reagent first chemically adsorbs CO and O2, and then desorbs CO2. The final CO concentration tends to 0, the O2 concentration remains stable, and the CO2 concentration decreases. This shows that the ablation agent has an impact on the changes in the CO and CO2 concentrations.


Assuntos
Dióxido de Carbono , Monóxido de Carbono , Dióxido de Carbono/análise , Carvão Mineral , Explosões , Gases
12.
Insects ; 13(4)2022 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-35447832

RESUMO

Spotted-wing drosophila, Drosophila suzukii (Matsumura), is a worldwide quarantine pest that is currently undergoing a rapid range expansion in the Americas, Europe, and parts of Africa. It feeds and breeds on soft-skinned fruits such as raspberries, blueberries, and cherries, and can cause significant economic losses to fruit production. This study investigated the occurrence of D. suzukii and its wild host fruits and parasitoids in Liaoning, Northeast China for the first time. Sentinel traps were used to monitor D. suzukii adults, and suspected fruits were collected weekly in four different locations (Wafangdian, Faku, Fengcheng, and Shenyang). The results showed that D. suzukii were distributed in the sweet soft-skinned fruit-production areas of Liaoning, and raspberry was the most infested fruit. During the field survey, four species of wild berries from non-crop habitats were found infested by D. suzukii, and two species of parasitoids (Leptopilina japonica and Asobara japonica) were collected. D. suzukii adult-population dynamics throughout the survey period (June to October) were similar in different survey locations; adult fly populations increased and peaked in August, and then declined until the fly was no longer detectable in October.

13.
Cancer Manag Res ; 14: 1507-1514, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35478711

RESUMO

Philadelphia chromosome positive B cell lymphoblastic lymphoma (Ph+ B-LBL) is an extremely rare disease. We report a 27-year-old patient diagnosed with primary testicular and cutaneous Ph+ B-LBL without bone marrow involvement. The CCCG-LBL-2016 regimen (https://clinicaltrials.gov/ct2/show/NCT02845882) was initially administered due to the fast pathological diagnosis as B-LBL that was first obtained. To identify potential therapeutic targets, RNA sequencing (RNAseq) was also performed on lymph node specimens as a part of the routine diagnostic workup in our center. Unexpectedly, IKZF1 deletions and BCR-ABL1 fusion transcripts were detected. Based on these results, we retrospectively performed fluorescence in situ hybridization (FISH) for BCR/ABL1 rearrangements in the same lymph node specimen, and a 70% positive signal was detected. The patient subsequently received the CCCG-LBL-2016 protocol combined with the BCR-ABL tyrosine kinase inhibitor (TKI) dasatinib, along with prophylactic intrathecal infusion. Then, the patient underwent TBI-based haploidentical (haplo) allogeneic hematopoietic stem cell transplantation (haplo-allo-HSCT) as consolidation following the achievement of remission and continued taking dasatinib as maintenance therapy. The patient was still in complete remission 1 year after diagnosis. This case indicates that the detection of potential molecular targets, especially those targets that can be pharmacologically treated, such as BCR/ABL1 fusion transcripts, is of important value to both LBL diagnosis and therapeutic strategy choices. FISH, reverse transcriptase polymerase chain reaction (RT-PCR) and/or RNAseq should be routinely carried out in lymphoma specimens to depict its genetic landscape for the further execution of a precise therapy strategy.

14.
Front Aging Neurosci ; 14: 870871, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35462690

RESUMO

This pilot study implements a hybrid brain computer interface paradigm based on motor imagery (MI) and steady-state visual evoked potential (SSVEP), in order to explore the neural mechanism and clinical effect of MI-SSVEP intervention paradigm on upper limb functional rehabilitation. In this study, EEG data of 12 healthy participants were collected, and the activation regions of MI-SSVEP paradigm were identified by power spectral density (PSD). By analyzing the inter trial phase consistency (ITPC) of characteristic regions and the causal relationship of brain network, the motor cognitive process including high-level somatosensory joint cortex in the intervention process of MI-SSVEP was studied. Subsequently, this study verified the clinical effect of MI-SSVEP intervention paradigm for 61 stroke patients. The results show that the robot assisted therapy using MI-SSVEP intervention paradigm can more effectively improve the rehabilitation effect of patients.

15.
Nanomedicine (Lond) ; 2022 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-35393861

RESUMO

Aim: The present study aimed to retrospectively compare the efficacy and safety between liposomal paclitaxel (Lps-P) and nanoparticle albumin-bound paclitaxel (Nab-P) in neoadjuvant systemic treatment (NST) of breast cancer. Materials & methods: 235 patients who were diagnosed with invasive breast cancer and then received dose-dense NST with epirubicin and cyclophosphamide followed by paclitaxel were enrolled. Results: Nab-P has an advantage in improving the total and axillary-only pathologic complete response rate over Lps-P. Although Nab-P can cause a higher incidence and severity of peripheral sensory neuropathy (PSN), most symptoms are temporary and reversible. In the Lps-P group, the proportion of patients with residual irreversible PSN is larger. Conclusion: Nab-P might be superior to Lps-P in NST of breast cancer.


Neoadjuvant systemic treatment (NST) is recommended for many patients with breast cancer before they undergo surgery to remove the cancer. This study retrospectively compared the efficacy and safety of two potential NST drugs, liposomal paclitaxel (Lps-P) and nanoparticle albumin-bound paclitaxel (Nab-P). Two hundred thirty-five patients participated in the study. These patients had been diagnosed with invasive breast cancer and were recommended NST with paclitaxel before surgery. The results showed that more participants who received Nab-P had no signs of cancer in their tissue samples from their breasts and armpit lymph nodes than participants who received Lps-P. Although Nab-P can cause a higher incidence and severity of peripheral sensory neuropathy (PSN), most symptoms are temporary and reversible. In conclusion, Nab-P might be superior to Lps-P for NST.

16.
Elife ; 112022 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-35439114

RESUMO

The dual-specificity tyrosine phosphorylation-regulated kinase DYRK2 has emerged as a critical regulator of cellular processes. We took a chemical biology approach to gain further insights into its function. We developed C17, a potent small-molecule DYRK2 inhibitor, through multiple rounds of structure-based optimization guided by several co-crystallized structures. C17 displayed an effect on DYRK2 at a single-digit nanomolar IC50 and showed outstanding selectivity for the human kinome containing 467 other human kinases. Using C17 as a chemical probe, we further performed quantitative phosphoproteomic assays and identified several novel DYRK2 targets, including eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) and stromal interaction molecule 1 (STIM1). DYRK2 phosphorylated 4E-BP1 at multiple sites, and the combined treatment of C17 with AKT and MEK inhibitors showed synergistic 4E-BP1 phosphorylation suppression. The phosphorylation of STIM1 by DYRK2 substantially increased the interaction of STIM1 with the ORAI1 channel, and C17 impeded the store-operated calcium entry process. These studies collectively further expand our understanding of DYRK2 and provide a valuable tool to pinpoint its biological function.

17.
Thromb Res ; 213: 128-136, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35366433

RESUMO

INTRODUCTION: Refractory or recurrent immune thrombocytopenia (ITP) patients suffer from the dual threat of high mortality and drug toxicity in addition to a very poor quality of life. Previous studies have shown that high mobility group box 1 (HMGB1) can promote the development of rheumatoid arthritis, systemic lupus erythematosus, and other autoimmune diseases. However, there is still a lack of research on the role of HMGB1 in the pathogenesis of ITP and whether it can be used as a predictor of efficacy and prognosis. METHODS: 20 patients of adult ITP with splenectomy were chosen as the experimental group, while 19 adults underwent splenectomy for traumatic splenic rupture without other diseases as the control group. We measured the expression of HMGB1, RORγt and Foxp3 in spleen tissues by immunohistochemistry. Another 50 patients, of which 20 were newly diagnosed without treatment and 30 were refractory ITP, and 25 healthy controls were enrolled to analyse the expression levels and mRNA levels of HMGB1, RORγt and Foxp3 in peripheral blood by Western blot and RT-qPCR. The expression of HMGB1, IL-17 and IL-10 in serum was assayed by ELISA. PBMCs from newly diagnosed ITP patients were cultured in vitro which stimulated with recombinant humanHMGB1 (rHMGB1) and its inhibitors, in which the expressions of RORγt and Foxp3 were measured. RESULTS: The expression of HMGB1 in the spleen with refractory ITP was significantly higher, while Foxp3 was decreased. A significant negative correlation was found between HMGB1 and Foxp3, and the overexpression of HMGB1 was significantly correlated with poor efficacy after splenectomy. The expression of HMGB1 and IL-17 increased and showed a positive correlation in serum, while IL-10 decreased and was negatively correlated with HMGB1. In PBMCs, the expression of HMGB1 and RORγt increased, while Foxp3 decreased, and the differences were more obvious in the refractory chronic ITP group. In a coculture system with PBMCs of untreated ITP patients, rHMGB1 increased RORγt expression and decreased Foxp3 expression, while an antiHMGB1 antibody partially corrected the above changes. CONCLUSION: Our results suggest that HMGB1 is associated with the imbalance of Treg/Th17 cells and is involved in the pathogenesis of ITP.


Assuntos
Proteína HMGB1/metabolismo , Púrpura Trombocitopênica Idiopática , Adulto , Fatores de Transcrição Forkhead/metabolismo , Humanos , Interleucina-10 , Interleucina-17/metabolismo , Membro 3 do Grupo F da Subfamília 1 de Receptores Nucleares/genética , Púrpura Trombocitopênica Idiopática/genética , Qualidade de Vida , Linfócitos T Reguladores
18.
HLA ; 2022 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-35436392

RESUMO

The novel HLA-C*08:242 allele was identified in an individual from the Sichuan province of China.

19.
Mol Genet Genomics ; 2022 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-35380267

RESUMO

Fibroblast growth factor 5 (FGF5), which is a well-established causative factor for blood pressure, has been identified as a susceptibility gene for preeclampsia (PE) in European and Central Asian women. Here, we examined whether polymorphism rs16998073 in FGF5 confer a significant risk to PE in Chinese Han population by case-control association analysis. FGF5 rs16998073 was genotyped by Sanger sequencing in women with preeclampsia (n = 187) and healthy controls (n = 229) of Han Chinese. We found the frequency of rs16998073T allele was significantly higher in PE patients than that in controls. Next, we utilized dual-luciferase reporter assays and electrophoretic mobility shift assay (EMSA) reactions to investigate whether rs16998073 different alleles could affect the transcriptional activity of FGF5. The dual luciferase reporter assay showed that T allele increased the transcriptional efficiency by 1.5-fold compared with the G allele. Similarly, EMSA revealed that the T allele had a strong transcription factor binding strength compared with the G allele. We then examined the mRNA and protein expression levels of FGF5 in placental tissues by real-time PCR and Western blot assays. We found FGF5 were significantly upregulated in placental tissues from PE patients or PE mouse model than their corresponding controls. In addition, in vitro cell experiments confirmed that FGF5 could promote cell apoptosis of HTR8/SVneo and inhibit cell invasion. Taken together, our data provide evidence implicating rs16998073 of FGF5 as a functional genetic risk variant for PE disease and FGF5 might participate in development of PE disease.

20.
Front Oncol ; 12: 840950, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35359369

RESUMO

Background: This study determined the predictive value of CRMP4 promoter methylation in prostate tissues collected by core needle biopsies for a postoperative upgrade of Gleason Score (GS) to ≥8 in patients with low-risk PCa. Method: A retrospective analysis of the clinical data was conducted from 631 patients diagnosed with low-risk PCa by core needle biopsy at multiple centers and then underwent Radical Prostatectomy (RP) from 2014-2019. Specimens were collected by core needle biopsy to detect CRMP4 promoter methylation. The pathologic factors correlated with the postoperative GS upgrade to ≥8 were analyzed by logistic regression. The cut-off value for CRMP4 promoter methylation in the prostate tissues collected by core needle biopsy was estimated from the ROC curve in patients with a postoperative GS upgrade to ≥8. Result: Multivariate logistic regression showed that prostate volume, number of positive cores, and CRMP4 promoter methylation were predictive factors for a GS upgrade to ≥8 (OR: 0.94, 95% CI: 0.91-0.98, P=0.003; OR: 3.16, 95% CI: 1.81-5.53, P<0.001; and OR: 1.43, 95% CI: 1.32-1.55, P<0.001, respectively). The positive predictive rate was 85.2%, the negative predictive rate was 99.3%, and the overall predictive rate was 97.9%. When the CRMP4 promoter methylation rate was >18.00%, the low-risk PCa patients were more likely to escalate to high-risk patients. The predictive sensitivity and specificity were 86.9% and 98.8%, respectively. The area under the ROC curve (AUC) was 0.929 (95% CI: 0.883-0.976; P<0.001). The biochemical recurrence (BCR)-free survival, progression-free survival (PFS), and cancer-specific survival (CSS) were worse in patients with CRMP4 methylation >18.0% and postoperative GS upgrade to ≥8 than in patients without an upgrade (P ≤ 0.002). Conclusion: A CRMP4 promoter methylation rate >18.00% in prostate cancer tissues indicated that patients were more likely to escalate from low-to-high risk after undergoing an RP. We recommend determining CRMP4 promoter methylation before RP for low-risk PCa patients.

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