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1.
Talanta ; 207: 120310, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31594599

RESUMO

Recently, superficially porous particles (SPPs) have been intensively studied and employed for highly efficient and fast separations. In this paper, the SiO2@SiO2 SPPs were synthesized by an improved polymerization-induced colloid aggregation (PICA) method using urea-formaldehyde polymer as the template. The agglomeration of silica core during modification with ureidopropyltrimethoxysilane (UPS) can not only be avoided by reflux in neutral ethanol solution, but also the secondary nucleation of the colloidal silica sol can be inhibited via optimizing the reaction conditions including pH, temperature, colloidal silica sol concentration and the reaction time. The shell thickness and pore size of SPPs can be controlled successfully by adjusting the weight ratio of silica core/colloidal silica sol and the particle size of colloidal silica sol, respectively. The SPP-C18 columns packed using octadecyltrichlorosilane (ODS) modified SPPs with different pore sizes were employed to separate small solutes and proteins. The baseline separations of 6 kinds of alkyl benzenes and 5 kinds of aromatic alcohol homologues were achieved within 4 min by the SPP-C18 column with 8 nm pore size. Compared with the commercial BEH-C18 column, more than 50,000/m of the plate number of propylbenzene was obtained, and the former provided higher column efficiency to separate small solutes than the latter. Meanwhile, 6 kinds of proteins were also separated completely within 2 min using the SPP-C18 column with 40 nm pore size. In addition, the SPP-C18 capillary column was applied to separate and identify the BSA/HeLa/mouse liver digests with capillary LC-MSMS, respectively. The results indicate that more proteins and peptides can be identified using SPP-C18 capillary column compared with commercial silica-C18 capillary column. The result demonstrates that the prepared SPP-C18 column provides higher column efficiency and the SPPs synthesized with the improved PICA method shows a great potential application for the fast separation of small solutes and proteins.

2.
J Pharm Biomed Anal ; 177: 112855, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31561061

RESUMO

FSH plays a key role in the function of the reproductive system of human beings and is widely used both diagnostically and therapeutically in reproductive medicine. With the growing incidence of infertility, the demand for FSH pharmaceutical products is increasing. For this reason, the quality control process for FSH products is becoming more stringent. An accurate determination of bioactivity is crucial for the safety and efficacy of recombinant human follicle stimulating hormone (rhFSH). Up to now, in-vivo bioassay based on FSH-induced increases in rat ovarian weight has been the only method widely accepted by different pharmacopoeias. However this method has such drawbacks as the complex procedures, long assay period and high variability. Here, we established a reporter gene assay (RGA) based on the CHO-K1-FSHR-CRE-Luc cell line that stably expresses human follicle stimulating hormone receptor (hFSHR), as well as a luciferase reporter under the control of cyclic adenosine monophosphate (cAMP) response elements (CRES). Our study showed that our new assay not only has good dose-dependent responsiveness to rhFSH, but it also performs excellently in terms of specificity, precision, linearity, and simplicity compared with in-vivo rat bioassays. These results implied that this robust reporter gene assay may be a viable supplement to the animal in-vivo bioassay and may be employed in potency determination of rhFSH pharmaceutical products.

3.
Sci Total Environ ; 698: 134264, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31494416

RESUMO

Phthalate esters (PAEs) have been shown to be ubiquitous in abiotic and biotic environmental compartments; however, information about bioaccumulation behavior and human exposure, both via environmental exposure and the diet, are limited. Herein, we report the concentrations and composition profiles of phthalate esters (PAEs) in biological samples, river water, indoor air, and outdoor air samples collected from an agricultural site in western China. Dibutyl phthalate (DNBP) occupied a relatively high abundance in biological samples, discrepant with the environmental samples in which di-(2-ethylhexyl) phthalate (DEHP) was the dominant congener. Significant correlations (P < 0.05) were observed between the biota and river water samples, indicating that river water heavily influenced PAE accumulation in biological samples. The mean log Bioaccumulation Factors (BAFs) varied from 0.91 to 2.96, which implies that most PAE congeners are not likely to accumulate in organisms. No obvious trends were observed between log octanol-water partition coefficient (KOW) and log BAF values, nor between log octanol-air partition coefficient (KOW) and biota-air accumulation factors (BAAFs). Nevertheless, the calculated log air-water partitioning factors (AWPFs) of diethyl phthalate (DEP), dimethyl phthalate (DMP), and butyl benzyl phthalate (BBP) were similar to predicted values whereas those for diisobutyl phthalate (DIBP), DNBP and DEHP were significantly higher. The estimated daily intakes of PAEs via food ingestion and environmental exposure were 15, 9.4 and 1.2 ng/kg-bw/day in toddlers, children and adults, respectively, laying at the low end of the reported data and well below the reference dose.

4.
Sci Total Environ ; 698: 134275, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31505352

RESUMO

With the pollution of perfluoroalkyl substances (PFASs) became increasingly serious, the researches focused on removal of PFASs by adsorption-photocatalysis method has attracted considerable attention. To make the catalyst TiO2 disperse uniformly as quantum dots onto hydrophobic surface which was liable to attract perfluorooctanoic acid (PFOA), the surfactant sodium dodecyl sulfate (SDS) were used in this work, which not only connected the hydrophilic TiCl3 to the hydrophobic sulfonated graphene (SG) nanosheets, but also behaved as the molecular template for controlled nucleation and growth of the nanostructured TiO2. After 3D SG-TiO2 QD nanosheets were fabricated, a series of 3D SG-TiO2 QD aerogels were self-assembled by ice-template. TiO2 uniformly distributed on the surface of SG aerogel at QD size level (2-3 nm) and the size of TiO2 could be effectively regulated by concentration of SDS. Compared with aggregated TiO2 material, 3D SG-TiO2 QD aerogels owned higher adsorption and photocatalytic performance. Benefiting from the hydrophobic surface of 3D SG as well as dispersed TiO2 QDs, 3D SG-TiO2 QD could enrich PFOA instantaneously (0.0381/s) and photocatalytic decomposed them effectively (1.898 E-4/s). PFOA degradation by hole and hydroxyl radicals proceeded via a stepwise mechanism. The column made of 3D SG-TiO2 QD could remove PFOA persistently in cycles of permeation. 3D SG-TiO2 QD possessed powerful adsorption-photocatalytic decomposition capability of PFOA and steady reusability performance. The present work highlights the individual roles and synergistic effect of TiO2 QD and 3D SG for effectively removing PFOA.

5.
Sci Total Environ ; 698: 134175, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31518786

RESUMO

Although jarosite and visible light are important factors for the formation of acid mine drainage (AMD), the effects of combined jarosite and visible light on chalcopyrite biodissolution have not been explored until now. In order to fill this knowledge gap, the combined effects of jarosite and visible light on chalcopyrite dissolution mediated by Acidithiobacillus ferrooxidans were investigated. The results indicated that jarosite and visible light could significantly accelerate chalcopyrite biodissolution, thus releasing more copper ions, iron ions and producing more acid. This in turn suggests enhanced generation of AMD under these conditions. Biodissolution results, mineral surface morphology, mineralogical phase and elemental composition analyses revealed that the promotion of chalcopyrite dissolution by additional jarosite and visible light was mainly attributed to the acceleration of ferric iron/ferrous iron cycling and the inhibition of the formation of a passivation layer (jarosite and Sn2-/S0) on the surface of chalcopyrite. This study provides a better understanding of the effects of jarosite and visible light on chalcopyrite biodissolution. In the future, the influences of jarosite and visible light on chalcopyrite dissolution should be considered in AMD evaluation to ensure reliability.

6.
Eur J Pharm Sci ; 141: 105124, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31669761

RESUMO

Influenza virus infections are a persistent threat to human health due to seasonal outbreaks and sporadic pandemics. Amantadine and rimantadine are FDA-approved influenza antiviral drugs and work by inhibiting the viral M2 proton channel. However, the therapeutic potential for the antiviral amantadine/rimantadine was curtailed by the emergence of drug-resistant mutations in its target protein M2. In this study, we identified four amantadine-resistant M2 mutants among avian and human influenza A H5N1 strains circulating between 2002 and 2019: the single S31N and V27A mutants, and the S31N/L26I and S31N/V27A double mutants. Herein, utilizing two-electrode voltage clamp (TEVC) assays, we screened a panel of structurally diverse M2 inhibitors against these single and double mutant channels. Three compounds 6, 7, and 15 were found to significantly block all three M2 mutants: M2-S31N, M2-S31N/L26I, and M2-S31N/V27A. Using recombinant viruses generated from reverse genetics, we further showed that these compounds also inhibited the replication of recombinant viruses harboring either the single S31N or double S31N/L26I and S31N/V27A mutants. This work represents the first example in developing antivirals by targeting the drug-resistant double mutants of M2 proton channels.

7.
J Cell Physiol ; 235(2): 900-908, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31245852

RESUMO

Genetic variation is considered to affect the N6 -methyladenosine (m6A) RNA transcript modification, which is the most prevalent posttranscriptional messenger RNA modification. This study aimed to identify m6A-associated single-nucleotide polymorphisms (m6A-SNPs) that may affect m6A methylation from numerous periodontitis (PD) SNPs. We identified an abundance of m6A-SNPs by analyzing raw data of published PD genome-wide association studies and m6A-SNPs list from the m6AVar database. Other evidence was found in public databases for expression quantitative trait loci (eQTL) and differential gene expression analysis. Accordingly, 1938 m6A-SNPs were identified, 104 of which appeared to be associated with PD (p < .05) while 65 showed eQTL signals. Lastly, the leading SNP rs2723183 (p = 3.93E-07) was predicted to regulate local gene IL-37 expression in PD (p = 2.64E-05; in GSE10334) and change regulatory motif RXRA. In summary, dozens of PD-associated m6A-SNPs were identified and their possible functions were demonstrated in this study.

8.
J Ethnopharmacol ; 246: 112212, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31494200

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: ANNAO tablets derive from Chinese classical prescriptions of Angong Niuhuang Pills with modified compositions, which have been singly or combined used for stoke associated neurological disorders. However the underlying mechanism is not yet well-defined, the present study investigated its anti-ischemic effects in rat middle cerebral artery occlusion (MCAO) model and focused on mitochondrial quality control. MATERIALS AND METHODS: Rats were subjected to 2 h of brain ischemia followed by 1 day or up to 7 days of reperfusion. Vehicle, ANNAO tablets or Edaravone were given at 1h after the start of reperfusion for 1 day or successive 7 days in MCAO rats. For the behavior assessment, Longa test and modified Neurological Severity Scores (m NSS) test were performed. Following the behavioral assessment, we assessed the protein expressions related to mitochondrial function. Moreover, we also assessed the neuroprotective effects of ANNAO tablets by immunohistochemical analysis. RESULTS: Compared with sham rats, ANNAO tablets improved the behavioral performance and decreased the infarction volume in the MCAO rats. Western blotting results showed that ANNAO tablets altered the expression level of multiple proteins related to mitochondrial function, elevated the ratio of Bcl-2/Bax and inhibited the apoptosis. Additionally, ANNAO tablets increased the number of NeuN positive neurons. CONCLUSIONS: The obtained data demonstrated that ANNAO tablets exhibited an obvious anti-cerebral ischemia-reperfusion effect, which could be attributed to the improvement of mitochondrial quality control.

9.
J Nanosci Nanotechnol ; 20(4): 2018-2024, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492207

RESUMO

Poly(ethylene glycol) (PEG)/poly(ethylene imine) (PEI) modified superparamagnetic iron oxide nanoparticles (SPIONs) were synthesized by the thermal decomposition of iron (III) acetylacetonate (Fe(acac)3) in PEG containing PEI. Transferrin (Tf) was employed to functionalize SPIONs. The potential of Tf-SPIONs as brain magnetic resonance (MR) imaging contrast agents was explored by using Kunming mice as an animal model. The in vivo experiments revealed that Tf-SPIONs exhibited an enhanced contrast time as compared with the PEG-SPIONs and PEG/PEI-SPIONs. Tf-SPIONs exhibit promising potential for bioimaging applications because of their advantages of dispersibility in water, low cytotoxicity and long circulation time in blood.

10.
Chemosphere ; 239: 124793, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31726530

RESUMO

Developing an efficient and environmentally friendly strategy for oil-water separation is extremely important for practical application. In this study, a superhydrophobic and superoleophilic melamine sponge loaded with cross-linked and swellable polydivinylbenzene was successfully fabricated by a facile and effective one-step impregnation-curing method with adhesion of polydimethylsiloxane. The prepared sponge not only exhibited high oil absorption capacity, but it also enabled rapid oil collection in situ, which could be extended to practical application. Moreover, the modified superhydrophobic sponge showed excellent mechanical resistance and chemical stability. The surface morphology and chemical composition were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. This material has great development potential for large-scale oil spill clean-up and chemical spill accidents.

11.
Chemosphere ; 240: 124919, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31726585

RESUMO

Ionic liquids (ILs) are regarded as green solvents and are frequently used in the chemical industry. However, ILs may impact plant growth if they are present in the soil environment. To compare toxicity of ILs with different anions in soil, three imidazolium-based ionic liquids (1-hexyl-3-methylimidazolium bromide, 1-hexyl-3-methylimidazolium nitrate, 1-hexyl-3-methylimidazolium tetrafluoroborate) were used to assess impact on Vicia faba. Following 10 d of exposure to these three ILs from 0 to 2500 mg kg-1, shoot length, root length and dry weight of Vicia faba were determined. Pot trials revealed that ILs inhibited Vicia faba growth and according to EC50 values, [C6mim]BF4 was the most toxic one. In addition, physiological indicators of Vicia faba were determined following 10 d of exposure at selected IL concentrations (0, 1, 10, 100 and 500 mg kg-1). ILs led to the generation of reactive oxygen species and then caused oxidative damage, including lipid peroxidation, protein damage and DNA damage, which triggered an increase in antioxidant content and enzyme activity. The experimental results indicated that oxidative stress may be the primary underlying toxic mechanism for Vicia faba. Furthermore, based on the data of physiological experiment, integrated biomarker response (IBR) was calculated to compare the toxicity of the three ILs and toxic order was: [C6mim]NO3<[C6mim]Br<[C6mim]BF4.

12.
Ecotoxicol Environ Saf ; 187: 109788, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31648073

RESUMO

The potential threats of microplastics to global health are a new problem. However, little is known about the influence of microplastics on soil organisms. Here, we investigated the effects of low-density polyethylene (LDPE, < 400 µm) on earthworms (Eisenia fetida) under different concentrations (0.1, 0.25, 0.5, 1.0, 1.5 g/kg dry) with three replicates in artificial soil. Results showed that surface damage of earthworms was observed at the concentration of 1.5 g/kg LDPE after exposure 28 days. The microplastics were ingested in a dose-response manner. Smaller sizes of LDPE microplastics were found in the casts of E. fetida, and approximately 30% of the microplastics egested (size < 100 µm) were increased compared with initial microplastics in the soil. The catalase activity and malondialdehyde content increased significantly at the concentration of 1.0 g/kg LDPE after exposure 28 days, and acetylcholine esterase was significantly stimulated at concentrations of 1.5 and 1.0 g/kg LDPE on days 21 and 28, respectively. The results of this study demonstrate the potential risk of LDPE microplastics to E. fetida and may provide a reference for the impact of microplastics on terrestrial creatures.

13.
Spectrochim Acta A Mol Biomol Spectrosc ; 224: 117400, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31437763

RESUMO

Water soluble protein content (WSPC) is a parameter of great significance to the soybean food industry. So far, genetic studies and breeding practices have been limited by the lack of a rapid technique for the evaluation of WSPC. Near-infrared reflectance spectroscopy (NIRS) is widely applied for rapid quantification of many traits, including moisture, protein and oil content, and dietary fiber. The present study aimed to establish and evaluate a NIRS regression model for the rapid prediction of WSPC in soybean. Results showed that seed coat color had a profound impact on the accuracy of protein content prediction, whereas the seed coat itself deeply influenced protein determination. We established a partial least squares (PLS) regression model with 167 soybean samples whose seed coat had been removed. Based on multiplicative scatter correction and Savitsky-Golay transformation, the highest determination coefficient (R2) was 0.831, and the relative predictive determinant was 2.417. Further analysis showed that seed roundness correlated negatively with WSPC (r=-0.59, P<0.001) and greatly impacted PLS regression model prediction accuracy. The PLS model was suitable only for intact seeds whose coat had been peeled off, but not for broken seeds, soy powder, and green cotyledon soybean seeds. This study highlights the effect the seed coat has on soybean composition determination by NIRS. Moreover, the established PLS model for soybean WSPC determination could facilitate genetic studies and breeding.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31493423

RESUMO

Two popular debilitating illness, unipolar depression (UD) and bipolar disorder (BD), have the similar symptoms and tight association on the psychopathological level, leading to a clinical challenge to distinguish them. In order to figure out the underlying common and different mechanism of both mood disorders, resting-state functional magnetic resonance imaging (rs-fMRI) data derived from 36 UD patients, 42 BD patients (specially type I, BD-I) and 45 healthy controls (HC) were analyzed retrospectively in this study. Functional brain networks were firstly constructed on both group and individual levels with a density 0.2, which was determined by a network thresholding approach based on modular similarity. Then we investigated the alterations of modular structure and other topological properties of the functional brain network, including global network characteristics and nodal network measures. The results demonstrated that the functional brain networks of UD and BD-I groups preserved the modularity and small-worldness property. However, compared with HC, reduced number of modules was observed in both patients' groups with shared alterations occurring in hippocampus, para hippocampal gyrus, amygdala and superior parietal gyrus and distinct changes of modular composition mainly in the caudate regions of basal ganglia. Additionally, for the network characteristics, compared to HC, significantly decreased global efficiency and small-worldness were observed in BD-I. For the nodal metrics, significant decrease of local efficiency was found in several regions in both UD and BD-I, while a UD-specified increase of participant coefficient was found in the right paracentral lobule and the right thalamus. These findings may contribute to throw light on the neuropathological mechanisms underlying the two disorders and further help to explore objective biomarkers for the correct diagnosis of UD and BD.

15.
Lancet Oncol ; 20(11): e619-e626, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31674320

RESUMO

As a result of recent, substantial capacity building, a new landscape for cancer drug trials is emerging in China. However, data on the characteristics of cancer drug trials, and how they have changed over time, are scarce. Based on clinical trials published on the China Food and Drug Administration Registration and Information Disclosure Platform for Drug Clinical Studies, we aimed to systematically review changes over time in clinical trials of cancer drugs in mainland China from 2009 to 2018, to provide insight on the effectiveness of the pharmaceutical industry and identify unmet clinical needs of stakeholders. A total of 1493 trials of 751 newly tested cancer drugs were initiated. Increases over time were observed for the annual number of initiated trials, newly tested drugs, and newly added leading clinical trial units, with a sharp increase after 2016. Of the 1385 trials in which cancer types were identified, solid tumours (325 [23%] trials), non-small-cell lung cancer (232 [17%]), and lymphoma (126 [9%]) were the most common. A markedly uneven distribution was also observed in the geography of leading units with the largest number of leading units located in east China (50 [41%]) and the smallest number located in southwest China (4 [3%]). The growth trends we observed illustrate the progress in and increasing capability of cancer drug research and development achieved in mainland China over the decade from 2009. The low number of clinical trials on tumours with epidemiological characteristics unique to the Chinese population and the unbalanced geographical distribution of leading clinical trial units will provide potential targets for policy makers and other stakeholders. Further research efforts should address cancers uniquely relevant to Chinese populations, globally rare cancers, and the balance between equitable drug access, efficiency, and sustainability of cancer drug research and development in mainland China.

16.
Neural Netw ; 122: 239-252, 2019 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-31726330

RESUMO

This paper presents theoretical results on the multistability of switched neural networks with commonly used sigmoidal activation functions under state-dependent switching. The multistability analysis with such an activation function is difficult because state-space partition is not as straightforward as that with piecewise-linear activations. Sufficient conditions are derived for ascertaining the existence and stability of multiple equilibria. It is shown that the number of stable equilibria of an n-neuron switched neural networks is up to 3n under given conditions. In contrast to existing multistability results with piecewise-linear activation functions, the results herein are also applicable to the equilibria at switching points. Four examples are discussed to substantiate the theoretical results.

17.
ACS Appl Mater Interfaces ; 11(47): 44466-44473, 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31691561

RESUMO

A metal-organic framework (MOF) [Cu(PDA)(DMF)] was synthesized under mild mixed solvothermal conditions. It is constructed by 1,10-phenanthroline-2,9-dicarboxylic acid (H2PDA) and Cu2+ ions. The complex exhibits high peroxidase-like activity and can catalytically oxidize the colorless substrate 3,3',5,5'-tetramethylbenzidine to a blue product in the presence of H2O2. However, the peroxidase-like activity of [Cu(PDA)(DMF)] can be potently inhibited in the presence of dopamine. Based on this phenomenon, the colorimetric detection of dopamine was demonstrated with good selectivity and high sensitivity. [Cu(PDA)(DMF)] showed good stability and robust catalytic activity, which has been employed in the detection of dopamine in human urine and pharmaceutical samples.

18.
J Agric Food Chem ; 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31709793

RESUMO

Astaxanthin (AST) is a fat-soluble and non-vitamin A source of carotenoid that can quench reactive oxygen species and it has strong antioxidant and anti-inflammatory abilities. Herein, we have used H2O2 to establish a model of oxidative damage to RAW 264.7 cells and cells treated with vitamin C as the positive control group. The changes in metabolome were examined using 1H NMR and the results demonstrated that H2O2 treatment and various metabolic pathways such as amino acid, glucose, and glycerolipid metabolism were downregulated, which in turn affected citric acid cycle and energy status. AST could reverse downregulation of some of these metabolic pathways to a certain extent, and reduce cellular oxidative stress and death. The AST group differed from the vitamin C group in regulating d-glutamine, d-glutamic acid, pyruvate, and glycerolipid metabolism. The experimental results help to further understand the antioxidant effects of AST.

19.
Immunol Lett ; 217: 49-55, 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31726188

RESUMO

BACKGROUND: Representing the first line of host defense against virus infections and an essential link between innate and adaptive immune response, the role of dendritic cells (DCs) in peripheral blood of juvenile-onset recurrent respiratory papillomatosis (JORRP) patients and association with disease activity were still not established. MATERIALS AND METHODS: In our present study, 28 JORRP patients and 28 age and sex matched healthy controls were enrolled. The percentage, phenotype and cytokine secretion of DC and was measured by flow cytometry. Plasma cytokine were detected by the enzyme-linked immunosorbent assay (ELISA). RESULTS: We found that the percentage of myeloid DC (mDC) was significantly lower in JORRP patients compared to healthy controls and was negatively correlated with interval times, but not surgical times or disease onset. Moreover, the activation marker, CD40 and CD86 was significantly up-regulated on the surfaces of mDC in JORRP patients compared with healthy controls. Neither the percentage nor activation of plasmacytoid DC (pDC) showed statistical difference between JORRP patients and healthy controls. HLA-DR expression on both mDC and pDC was down-regulated in JORRP group and negatively correlated with surgical times. Antigen presenting ability of DC was greatly impaired in JORRP patients of higher number of operations and shorter interval time. Plasma IL-10 as well as IL-10 secreted by mDC was higher in JORRP patients compared with healthy control. Finally, we detected an up-regulated TLR2 and TLR4 expression on mDCs and TLR4 expression was positively correlated with HLA-DR expression on mDC of JORRP patients. CONCLUSION: Our results demonstrate an abnormal TLR2 and TLR4 expression in mDCs may contribute to suppressive immune response to HPV6 or HPV11 infection and associated with disease activity in JORRP patients.

20.
J Adv Nurs ; 2019 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-31736133

RESUMO

AIMS: To investigate workplace violence and nurse outcomes by comparing gender differences. DESIGN: A secondary analysis of cross-sectional survey data. METHODS: Workplace violence was measured by four items from the International Hospital Outcome Study. Nurse outcomes were measured by tools including burnout, job satisfaction and intention to stay. We used propensity score matching to generate a sociodemographic balanced dataset of 108 male and 288 female nurses. A hypothetical relationship model was derived from the affective events theory. Comparative statistics and multi-group structural equation modelling were conducted to analyze gender differences. Data were collected in China from December 2013 - August 2014. RESULTS: Male nurses reported more workplace violence from staff and less intention to stay than females. Besides finding the mediation of burnout sharing with female nurses consistent with the affective events theory, workplace violence was directly linked to less intention to stay in male nurses. CONCLUSION: Male nurses experience more workplace violence by staff than female nurses. Besides responding emotionally to workplace violence like female nurses, male nurses also respond behaviourally. IMPACT: What problem did the study address? Gender differences in workplace violence and its relationship to nurse outcomes. What were the main findings? Male nurses experienced more workplace violence than female nurses, linked directly to less intention to stay. Workplace violence linked to less job satisfaction and intention to stay in nurses was mediated by burnout. Where and on whom will the research have impact? Gender-based prevention of and coping with workplace violence should be included in nursing training.

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