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1.
J Orthop Translat ; 38: 117-125, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36381249

RESUMO

Objectives: This study assessed the efficacy, safety, pharmacokinetics (PK), and immunogenicity profiles of a denosumab biosimilar (LY06006) in Chinese postmenopausal osteoporotic women with a high risk of fracture. Methods: In this multicenter, randomized, double-blind, placebo-controlled, phase 3 trial, 448 postmenopausal women aged 50-85 years with osteoporosis were enrolled at 49 centers in China and were randomly assigned (3:1) to receive 60 â€‹mg of the denosumab biosimilar (LY06006) or placebo subcutaneously every 6 months for 1 year. Lumbar spine bone mineral density (BMD) change was the primary endpoint. Results: Of the 448 randomized patients, 409 (LY06006, n â€‹= â€‹311; placebo, n â€‹= â€‹98) completed the study. All 448 (100.0%) subjects were included in the intent-to-treat (ITT) trial, 427 (95.3%) were included in the full analysis set (FAS), 408 (91.1%) were included in the per protocol set (PPS), 446 (99.6%) were included in the safety set (SS), and 336 (75.0%) were included in the pharmacokinetics concentration set (PKCs). For the primary endpoint, a 4.71% (95% CI, 3.81%, 5.60%) treatment difference in percent change in lumbar spine BMD from baseline to month 12 was observed in the LY06006 group compared with the placebo group (P â€‹< â€‹0.0001). For the secondary endpoints, LY06006 was associated with increased lumbar spine BMD levels measured at month 6, BMD levels at the femoral neck, total hip, and trochanter measured at months 6 and 12 and reduced serum C-terminal telopeptide of type 1 collagen (CTX) and procollagen type 1 â€‹N-peptide (P1NP) levels at months 1, 6, and 12. Safety analysis was based on the safety analysis set (SS), and 264 (78.6%) subjects in the LY06006 group and 83 (75.5%) in the placebo group experienced adverse events (AEs). Most events were mild or moderate and not related to the study drugs. Conclusion: In postmenopausal women with a high risk of fracture, LY06006 increased the BMD and decreased bone resorption; thus, LY06006 might be an effective treatment for osteoporosis. LY06006 was generally safe and well tolerated without unexpected adverse reactions, similar to the reference drug Prolia®. The characteristics of effectiveness and safety were similar to those reported in previous studies. The translational potential of this article: In this multi-center, randomized, double-blind, placebo-controlled phase 3 study, LY06006 showed substantially efficacy to increase BMD and well tolerance without unexpected adverse reactions, which is comparable to the reference drug Prolia ®. The presented results are encouraging and can offer some valuable evidence for the clinical practice.

2.
Behav Brain Res ; 437: 114133, 2023 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-36179805

RESUMO

The incomplete understanding of mild traumatic brain injury (MTBI)-related cognitive impairment in the acute stage and the low cognitive needs of patients in the later stage might be the main reasons for the neglect of clinical symptoms in patients with MTBI. Patients often experience attention deficits; however, it is unclear whether these patients suffer from general deficits or selective impairment of the brain attention network. Therefore, we investigated deficits in the attention function of patients with mild brain traumatic injury. Patients (n = 50) and matched healthy controls (n = 49) completed a general neuropsychological background test and the Attention Network Test, which provided an independent assessment of the three attention networks (alerting, orienting, and executive control). We found that patients had significant deficits in the orienting network but none in the alerting and executive control networks. Furthermore, patients' cognitive task scores in attention, memory, and information processing tasks were significantly lower than the scores of the controls. Our results demonstrated that patients with MTBI had selective impairment in the orienting network and extensive cognitive impairments, including those related to general attention, memory, and information processing speed.


Assuntos
Concussão Encefálica , Humanos , Concussão Encefálica/complicações , Tempo de Reação , Testes Neuropsicológicos , Função Executiva , Cognição
3.
Bioact Mater ; 21: 464-482, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36185748

RESUMO

The inertness of synthetic polymer materials and the insufficient mechanical strength of reprocessed decellularized extracellular matrix (dECM) limited their promotive efforts on tissue regeneration. Here, we prepared a hybrid scaffold composed of PCL microfibers and human placental extracellular matrix (pECM) nanofibers by co-electrospinning, which was grafted with heparin and further absorbed with IL-4. The hybrid scaffold with improved hemocompatibility firstly switched macrophages to anti-inflammatory phenotype (increased by 18.1%) and then promoted migration, NO production, tube formation of endothelial cells (ECs), and migration and maturation of vascular smooth muscle cells (VSMCs), and ECM deposition in vitro and in vivo. ECs coverage rate increased by 8.6% and the thickness of the smooth muscle layer was 1.8 times more than PCL grafts at 12 wks. Our study realized the complementary advantages of synthetic polymer materials and dECM materials, and opened intriguing perspectives for the design and construction of small-diameter vascular grafts (SDVGs) and immune-regulated materials for other tissue regeneration.

4.
HGG Adv ; 4(1): 100146, 2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36262216

RESUMO

Huntington's disease (HD) is an autosomal dominant neurodegenerative disease caused by CAG trinucleotide repeat expansions in exon-1 of huntingtin (HTT). Currently, there is no cure for HD, and the clinical care of individuals with HD is focused on symptom management. Previously, we showed allele-specific deletion of the expanded HTT allele (mHTT) using CRISPR-Cas9 by targeting nearby (<10 kb) SNPs that created or eliminated a protospacer adjacent motif (PAM) near exon-1. Here, we comprehensively analyzed all potential PAM sites within a 10.4-kb genomic region flanking exon-1 of HTT in 983 individuals with HD using a multiplex targeted long-read sequencing approach on the Oxford Nanopore platform. We developed computational tools (NanoBinner and NanoRepeat) to de-multiplex the data, detect repeats, and phase the reads on the expanded or the wild-type HTT allele. One SNP common to 30% of individuals with HD of European ancestry emerged through this analysis, which was confirmed as a strong candidate for allele-specific deletion of the mHTT in human HD cell lines. In addition, up to 57% HD individuals may be candidates for allele-specific editing through combinatorial SNP targeting. Cumulatively, we provide a haplotype map of the region surrounding exon-1 of HTT in individuals affected with HD. Our workflow can be applied to other repeat expansion diseases to facilitate the design of guide RNAs for allele-specific gene editing.

5.
Food Chem ; 403: 134136, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36194932

RESUMO

Oxidative stress is a major cause of cardiovascular diseases (CVDs). Antioxidant peptides have potential to improve CVDs. The aim of this study was to develop novel antioxidant peptides from silver carp muscle hydrolysates (SCHs) and investigate their gastrointestinal digestion (GID) stability, and bioavailability. Results showed that SCHs generated by Alcalase and Papain exhibited the highest free radical scavenging activity, peroxynitrite scavenging activity and low density lipoprotein (LDL) oxidation inhibitory capacity. All these antioxidant activities were enhanced in different SCHs after GID. Notably, the GID products of Papain-SCH and Alcalase-SCH possessed potent ameliorating effects on oxidative stress injury in endothelial cells. After transepithelial transport, total of nine peptides was identified from permeates of GID products of Papain-SCH and Alcalase-SCH. The VKVGNEF and MEAPPH were both novel peptides in the current study that possess the highest activities in scavenging free radicals, inhibiting LDL oxidation and alleviating oxidative stress injury in endothelial cells.


Assuntos
Antioxidantes , Carpas , Animais , Antioxidantes/farmacologia , Antioxidantes/química , Hidrolisados de Proteína/química , Papaína/química , Células Endoteliais , Peptídeos/farmacologia , Peptídeos/química , Subtilisinas/química , Músculos , Digestão
6.
Sci Total Environ ; 856(Pt 1): 159081, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36179843

RESUMO

Simultaneous nitrification and denitrification (SND) has the advantage of energy saving and carbon demand reduction. Here, readily available low-cost iron scraps packing was added to an aerobic sludge system. This successfully enhanced the efficiency of total nitrogen removal from 37.7 ± 13.2 % to 62.7 ± 7.9 % over 2 days. While electrons from iron biocorrosion did not contribute to nitrate reduction, iron promoted heterotrophic denitrification. The iron scraps changed the spatial distribution of the microbial community, where more denitrification bacteria accumulated around the packing and higher denitrification capacity was noted. Metagenomic analysis of the sludge cultured in the presence of iron scraps for 2 days revealed that, with the exception of the enriched amoA/B/C gene expression, the abundance of other key nitrogen removal genes showed little variation. Furthermore, the structure of the microbial community was unchanged probably due to the relatively short culturing period. However, metatranscriptomic analysis indicated that iron increased the abundance of nitrifying bacteria (i.e. unclassified Nitrosomonas, Nitrosomonas sp. Is79A3 and Nitrospira defluvii) and promoted higher expression of nitrification genes. Notably, iron scraps packing decreased the abundance of the key denitrification bacteria Thauera sp. MZ1T from 52.92 to 7.58 %. The expression of napA/B also decreased, while expression of narG/H/I increased by 9 to 23 fold and a 2 to 3 fold over expression was noted for nirS, norB/C and nosZ in the presence of iron scraps. This suggested that aerobic denitrification was inhibited and anaerobic denitrification was promoted. This study has provided in-depth understanding of the influence of iron on SND to improve the application of iron-supported biological processes.


Assuntos
Nitrogênio , Esgotos , Desnitrificação , Ferro , Reatores Biológicos , Nitrificação , Águas Residuárias
7.
Schizophr Bull ; 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36318234

RESUMO

BACKGROUND AND HYPOTHESIS: Schizophrenia manifests with marked heterogeneity in both clinical presentation and underlying biology. Modeling individual differences within clinical cohorts is critical to translate knowledge reliably into clinical practice. We hypothesized that individualized brain atrophy in patients with schizophrenia may explain the heterogeneous outcomes of repetitive transcranial magnetic stimulation (rTMS). STUDY DESIGN: The magnetic resonance imaging (MRI) data of 797 healthy subjects and 91 schizophrenia patients (between January 1, 2015, and December 31, 2020) were retrospectively selected from our hospital database. The healthy subjects were used to establish normative reference ranges for cortical thickness as a function of age and sex. Then, a schizophrenia patient's personalized atrophy map was computed as vertex-wise deviations from the normative model. Each patient's atrophy network was mapped using resting-state functional connectivity MRI from a subgroup of healthy subjects (n = 652). In total 52 of the 91 schizophrenia patients received rTMS in a randomized clinical trial (RCT). Their longitudinal symptom changes were adopted to test the clinical utility of the personalized atrophy map. RESULTS: The personalized atrophy maps were highly heterogeneous across patients, but functionally converged to a putative schizophrenia network that comprised regions implicated by previous group-level findings. More importantly, retrospective analysis of rTMS-RCT data indicated that functional connectivity of the personalized atrophy maps with rTMS targets was significantly associated with the symptom outcomes of schizophrenia patients. CONCLUSIONS: Normative modeling can aid in mapping the personalized atrophy network associated with treatment outcomes of patients with schizophrenia.

8.
RSC Adv ; 12(43): 27963-27969, 2022 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-36320274

RESUMO

In this study, we report a one-pot synthesis and enzyme-responsiveness of polyethylene glycol (PEG) and glutamic acid (Glu)-based amphiphilic doxorubicin (DOX) prodrug nanomicelles for cancer therapeutics. The nanomicelles were accomplished by esterification and amidation reactions. The nuclear magnetic resonance (NMR) and Fourier transform infrared (FTIR) data confirmed the structure of nanomicelles. The DOX-loaded nanomicelles showed a DLS-measured average size of 107 nm and excellent stability in phosphate-buffered saline (PBS) for 7 days. The drug loading and cumulative release rates were measured by ultraviolet-visible (UV-vis) spectrophotometry at 481 nm. The cumulative release rate could reach 100% in an enzyme-rich environment. Further, the therapeutic efficiency of nanomicelles to cancer cells was determined by cell viability and cellular uptake and distribution using HeLa cells. The cell viability study showed that the DOX-loaded nanomicelles could effectively inhibit the HeLa cell proliferation. The cellular uptake study confirmed that the nanomicelles could be effectively ingested by HeLa cells and distributed into cell nuclei. Based on the collective experimental data, this study demonstrated that the synthesized nanomicellar prodrug of DOX is a potential candidate for cancer therapeutics.

9.
RSC Adv ; 12(44): 28295-28298, 2022 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-36320509

RESUMO

A simple and practical method for the construction of 1,3,5-trisubstituted imidazolidine derivatives via [3 + 2] cycloaddition reaction has been developed. This reaction could smoothly proceed between nonstabilized azomethine ylides generated in situ and 2-benzothiazolamines to deliver a wide scope of differently substituted imidazolidines in high yields (up to 98%). The structure of one example was confirmed by X-ray single-crystal structure analysis.

10.
Chem Biodivers ; : e202200805, 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36328766

RESUMO

Clinical notes from electronic health records (EHRs) contain a large amount of clinical phenotype data on patients that can provide insights into the phenotypic presentation of various diseases. A number of Natural Language Processing (NLP) algorithms have been utilized in the past few years to annotate medical concepts, such as Human Phenotype Ontology (HPO) terms, from clinical notes. However, efficient use of NLP algorithms requires the use of high-quality clinical notes with phenotype descriptions, and erroneous annotations often exist in results from these NLP algorithms. Manual review by human experts is often needed to compile the correct phenotype information on individual patients. Here we develop TermViewer, a web application that allows multi-party collaborative annotation and quality assessment of clinical notes that have already been processed and tagged by NLP algorithms. TermViewer allows users to view clinical notes with HPO terms highlighted, and to easily classify high-quality notes and revise incorrect tagging of HPO terms. Currently, TermViewer combines MetaMap and cTAKES, two of the most widely used NLP tools for tagging medical terms, and identifies where these two tools agree and disagree, allowing users to perform collaborative manual reviews of computationally generated HPO annotations. TermViewer can be a stand-alone tool for analyzing notes or become part of a machine-learning pipeline where tagged HPO terms can be used as additional input data. TermViewer is available at https://github.com/WGLab/TermViewer.

11.
Artigo em Inglês | MEDLINE | ID: mdl-36328894

RESUMO

BACKGROUND: Liver transplantation (LT) is an effective treatment option for end-stage liver disease. Mammalian target of rapamycin (mTOR) inhibitors, such as rapamycin, are widely used post LT. DATA SOURCES: In this review, we focused on the anti-cancer activities and metabolic side effects of rapamycin after LT. The literature available on PubMed for the period of January 1999-September 2022 was reviewed. The key words were rapamycin, sirolimus, liver transplantation, hepatocellular carcinoma, diabetes, and lipid metabolism disorder. RESULTS: Rapamycin has shown excellent effects and is safer than other immunosuppressive regimens. It has exhibited excellent anti-cancer activity and has the potential in preventing hepatocellular carcinoma (HCC) recurrence post LT. Rapamycin is closely related to two long-term complications after LT, diabetes and lipid metabolism disorders. CONCLUSIONS: Rapamycin prevents HCC recurrence post LT in some patients, but it also induces metabolic disorders. Reasonable use of rapamycin benefits the liver recipients.

12.
Artigo em Inglês | MEDLINE | ID: mdl-36321392

RESUMO

Covalent organic frameworks (COFs) have been extensively investigated due to their unique structure, porosity, and functionality. However, at the topological level, COFs remain as two-dimensional (2D) or three-dimensional (3D) structures, while COFs with one-dimensional (1D) topology have not been systematically explored. In this work, we proposed a synthetic strategy for the construction of 1D-COFs based on non-linear edges and suitable high-symmetry vertices. Compared with their 2D-COFs counterparts, the 1D-COFs with AIEgens located at the vertex of the frame exhibited enhanced fluorescence. The density functional theory (DFT) calculations revealed that the dimensional-induced rotation restriction (DIRR) effect could spontaneously introduce additional non-covalent interactions between the strip frames, which could substantially diminish non-radiative transitions. This work also provides protocols for the design of 1D-COFs and a guidance scheme for the synthesis of emitting COFs.

13.
Laryngoscope ; 2022 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-36321782

RESUMO

OBJECTIVES: To explore the prevalence of hypopharyngeal carcinoma (HPC) with synchronous second primary malignancies (Syn-SPMs), their impact on clinical outcomes, and associated risk factors in the image-enhanced endoscopy era. MATERIALS AND METHODS: We retrospectively analyzed 673 patients newly diagnosed with HPC at our cancer center between 2009 and 2019. The patients were divided into three groups: (a) no second primary malignancies (N-SPMs, n = 533); (b) synchronous carcinoma in situ (Syn-Tis, n = 60); (c) synchronous invasive tumors (Syn-invasive, n = 80). Propensity score matching was conducted to balance the N-SPMs and Syn-invasive groups at a 3:1 ratio. RESULTS: Most (96.1%) underwent pretreatment esophagogastroduodenoscopy evaluation with image-enhanced endoscopy. The incidence rates were: Syn-SPMs, 20.8%; Syn-Tis, 8.9%; Syn-invasive, 11.9%. At a median follow-up of 66.7 months, the Syn-Tis and N-SPMs groups had a similar 5-year overall survival (OS; 45.6% vs. 44.5%; hazard ratio [HR], 0.956; 95% confidence interval [CI], 0.660-1.385; p = 0.806). Compared to the N-SPMs group, the Syn-invasive group had poorer 5-year OS (27.0% vs. 52.9%; HR, 2.059; 95% CI, 1.494-2.839; p < 0.001). Alcohol consumption was significantly associated with Syn-SPMs occurrence (odds ratio, 2.055, 2.414, and 3.807 for light, intermediate, and heavy drinkers, respectively). CONCLUSION: The prevalence of Syn-SPMs among patients with HPC was high. Syn-invasive SPMs decreased the survival of patients with HPC. Routine screening with image-enhanced endoscopy should be recommended to detect early-stage SPMs, especially for heavy alcohol drinkers. LEVEL OF EVIDENCE: III Laryngoscope, 2022.

14.
Front Neurosci ; 16: 905942, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36330349

RESUMO

Background: The impact of migraine without aura (MWoA) on cognitive function remains controversial, especially given the sparse literature on emotional memory. Methods: Twenty seven MWoA patients and 25 healthy controls (HCs) were enrolled in this cross-sectional study. Emotional memory behavior was evaluated by combining incidental encoding with intentional encoding of five emotional categories of visual stimulus [positive valence + high arousal (PH), negative valence + high arousal (NH), positive valence + low arousal (PL), negative valence + low arousal (NL), and neutral (N)]. The recollection performance (Pr) was measured and compared. Then, the neural relevance was explored by correlating the Pr with gray matter volume (GMV) and resting-state functional connectivity (rs-FC) based on structural and functional magnetic resonance imaging. Results: No significant differences in recollection performance or emotional enhancement of memory effect were observed. However, MWoA patients were more sensitive to the valence and arousal of emotional stimuli under incidental encoding. Significantly, the Pr-PH under incidental encoding and Pr-PL under intentional encoding were negatively correlated with the GMV of the left precuneus, and the rs-FC between the left precuneus and putamen was positively correlated with Pr-PL under intentional encoding in MWoA patients. Conclusion: Our study demonstrated the tendency for the influence of migraine on emotional memory and revealed the left precuneus as a critical contributor to recollection performance, providing novel insights for understanding emotional memory and its neural mechanisms in MWoA patients.

16.
iScience ; 25(11): 105465, 2022 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-36338432

RESUMO

To overcome the increased risk of SARS-CoV-2 reinfection or post-vaccination infection caused by the Omicron variant, Omicron-specific vaccines were considered a potential strategy. We reported the increased magnitude and breadth of antibody response against VOCs elicited by post-vaccination Delta and Omicron infection, compared to WT infection without vaccination. Then, in mouse models, three doses of Omicron-RBD immunization elicited comparable neutralizing antibody (NAb) titers with three doses of WT-RBD immunization, but the neutralizing activity was not cross-active. By contrast, a heterologous Omicron-RBD booster following two doses of WT-RBD immunization increased the NAb titers against Omicron by 9-folds than the homologous WT-RBD booster. Moreover, it retains neutralization against both WT and current VOCs. Results suggest that Omicron-specific subunit booster shows its advantages in the immune protection from both WT and current VOCs and that SARS-CoV-2 vaccines including two or more virus lineages might improve the NAb response.

17.
Food Chem ; 405(Pt A): 134855, 2022 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-36368102

RESUMO

Characterization and bioactivity of A-type procyanidins was investigated in litchi fruitlet (LF) at different stages and mature pericarp (MP) of 5 litchi cultivars. The content of total phenols in LFs was higher than that of MP and showed good antioxidant activity. Eleven procyanidins were identified in samples, including procyanidin A2, procyanidin A4, and 1 dimer, 2 trimers, and 1 tetramer of A-type procyanidin. Also, A-type procyanidin could stably exist in LFs stage, but declined substantially after maturity, which was about 1.45 - 3.56 times than mature pericarp. In addition, the second stage of LFs showed strong anti-inflammatory and anti-proliferative activities, in which monomer and A-type procyanidin trimers in LFs were significantly correlated with antioxidant (r > 0.72; p < 0.01) and anti-inflammatory (r = 0.53; p < 0.05) activities, respectively. Therefore, litchi in LF stage could be a good source of A-type oligomer procyanidins which had good application value.

18.
Mol Biol Rep ; 2022 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-36370298

RESUMO

BACKGROUND: By the time our study was completed, the chloroplast genomes of Syringa oblata, S. pubescents subsp. Microphylla, and S. reticulate subsp. Amurensis had not been sequenced, and their genetic background was not clear. THE RESEARCH CONTENT: In this study, the chloroplast genomes of Syringa oblata, S. pubescents subsp. Microphylla, S. reticulate subsp. Amurensis, and five other species of Syringa were sequenced for a comparative genomics analysis, inverted repeat (IR) boundary analysis, collinearity analysis, codon preference analysis and a nucleotide variability analysis. Differences in the complete chloroplast genomes of 30 species of Oleaceae were compared with that of S. oblata as the reference species, and Ginkgo biloba was used as the out group to construct the phylogenetic tree. RESULTS: The results showed that the chloroplast genomes of S. oblata, S. pubescents subsp. Microphylla, and S. reticulate subsp. Amurensis were similar to those of other angiosperms and showed a typical four-segment structure, with full lengths of 155,569, 160,491, 155,419, and protein codes of 88, 95, and 87, respectively. Because the IR boundary of S. pubescents subsp. Microphylla was significantly expanded to the large single copy (LSC) region, resulting in complete replication of some genes in the IR region, the LSC region of S. pubescents subsp. Microphylla was significantly shorter than those of S. oblate and S. reticulate subsp. Amurensis. Similar to most higher plants, these three species have a preference for their codons ending with A/T. CONCLUSIONS: We consider the genus Syringa to be a synphyletic group. The nucleotide variability and phylogenetic analyses showed that Syringa differentiated before Ligustrum and Ligustrum developed from Syringa. We propose removing the existing section division and directly dividing Syringa into five series.

19.
Front Oncol ; 12: 955150, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36353544

RESUMO

Although vaginal microbiota (VM) may interact with human papillomavirus (HPV) infection and clearance, longitudinal data remain very limited. We aimed to investigate the association between VM at baseline and the clearance of high-risk HPV (HR-HPV) infection within 12 months. Cervical swabs were collected at diagnosis from 85 patients with HR-HPV infection and histologically confirmed cervical lesions, including cervicitis, low-grade squamous intraepithelial lesion and high-grade squamous intraepithelial lesion. Microbiome analysis was performed using 16S rRNA gene sequencing. Among the 73 women included in the analyses, HPV clearance was observed in 58.9% of the patients within 12 months. No significant difference was observed between the HPV-cleared and HPV-uncleared groups regarding age, disease stage, HPV subtype, VM community state types, and VM diversity (α and ß). Women with the depletion of enterococcus ASV_62 and enrichment in Lactobacillus iners at baseline were less likely to have HPV clearance at month 12. Further analysis revealed a significant negative association between high abundance of L. iners and HPV clearance in patients who received non-operative treatment (OR = 3.94, p = 0.041), but not in those who received operative treatment (OR = 1.86, p = 0.660). Our findings provide new evidence for the potential role of VM in the persistent HR-HPV infections.

20.
Diabetes Metab Syndr Obes ; 15: 3459-3468, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36353667

RESUMO

Objective: To evaluate associations of obesity indices with bone mineral densities (BMD) and risk of osteoporosis in T2DM patients totally and stratified across presence of any diabetic cardiovascular complications. Methods: Cross-sectional analyses of baseline information on a cohort of 250 T2DM patients were conducted in Xiamen, China. Obesity indices included body weight, height, body mass index (BMI), waist and waist hip ratio (WHR). BMD was measured using dual-energy X-ray absorptiometry at three different sites, and osteoporosis was defined based on the minimum T-scores of BMD. Presence of any diabetic vascular complications was confirmed by checking their medical records histories. Results: Among the 250 T2DM patients, 50 (20.0%) were defined as osteoporosis. Multivariable linear regression and multivariable logistic regression analyses showed that igher obesity indices, including body weight, BMI and waist, but neither body height nor waist hip ratio, were positively associated with the minimum T-scores of BMD and had significantly decreased risk of osteoporosis. Stratified analyses across presence of any of diabetic vascular complications showed similar results for those with any of diabetic vascular complications, while no significant association between obesity indices and minimum T-scores of BMD was found for those without. Postmenopausal women (vs men) and ever drinking were significantly associated with increased risk of osteoporosis, and the adjusted odds ratios (95% CIs) were 5.165 (1.762-15.138, p = 0.003) and 3.789 (1.087-13.214, p = 0.037), respectively. None of metabolic profiles, including systolic and diastolic blood pressure, triglyceride, total cholesterol, high-density lipoprotein cholesterol, HbA1c and blood uric acid, was significantly associated with either minimum T-scores of BMD or risk of osteoporosis. Conclusion: Associations of obesity indices with either BMD or risk of osteoporosis in T2DM patients varied by presence of any diabetic vascular complication and should be not interpreted as causal without considering the often-unmeasured effect modification by health status.

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