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1.
Clin Chim Acta ; 455: 75-9, 2016 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-26826395

RESUMO

BACKGROUND: Primary immune thrombocytopenic purpura (pITP) is defined as isolated autoimmune thrombocytopenia with idiopathic low platelet count, normal bone marrow, and unexplained causes of thrombocytopenia. Currently there is no definite criterion for ITP diagnosis. METHODS: We conducted proteomic screen of patients with pITP, secondary immune thrombocytopenia (sITP), and healthy controls using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). The proteomic profiles were obtained from platelet lysate samples of 82 healthy adult controls, 64 pITP, and 70 sITP patients, from which we screened marker proteins with significant differences, and constructed a diagnosis model using the artificial neural network (ANN) technique. RESULTS: We identified 6 marker proteins in the platelet lysates of pITP patients. This diagnosis method differentiated pITP patients from sITP effectively with a sensitivity of 96.9% (31/32), a specificity of 71.0% (54/76), and the area under the ROC curve of 0.864 in the training set, and a sensitivity of 87.5% (28/32), a specificity of 69.7% (53/76), and a positive predictive value of 75.0% (81/108) in the test set. CONCLUSION: The artificial neural network model based on platelet protein profiling established a potential pITP diagnosis platform.


Assuntos
Plaquetas/metabolismo , Proteômica , Púrpura Trombocitopênica Idiopática/sangue , Adulto , Biomarcadores/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Púrpura Trombocitopênica Idiopática/diagnóstico , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
2.
Cell Biochem Biophys ; 72(3): 799-805, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25656768

RESUMO

This study was devised to identify potential biomarkers of schizophrenia (SP) using proteomics techniques. We obtained 44 serum specimens from patients with SP, 26 specimens from patients with depression, and 40 specimens from healthy controls. Immobilized metal affinity capture protein chips (IMAC30) and surface-enhanced laser desorption-ionization time-of-flight mass spectrometry were used to isolate and obtain mass spectrometric data of differentially expressed serum proteins. The sequences of the peaks discrepant among the study groups were obtained using matrix-assisted laser desorption/ionization mass spectrometry and proteins identified using Mascot database. In the SP group, there were 91 protein peaks that were different from other study groups at the p value of <0.05 and 54 peaks different at the p value of <0.01. Two protein peaks at the mass-to-charge ratio of 1,207.41 and 1,466.78 were markedly different among the study groups, with the lowest expression in specimens from patients with SP. The amino acid sequences were, respectively, Glu-Gly-Asp-Phe-Leu-Ala-Glu-Gly-Gly-Gly-Val-Arg (EGDFLAEGGGVR) and Asp-Ser-Gly-Glu-Gly-Asp-Phe-Leu-Ala-Glu-Gly-Gly-Gly-Val-Arg (DSGEGDFLAEGGGVR). These proteins were identified as the N-terminal fragments of fibrinogen. In conclusion, these biomarker proteins may be useful for molecular diagnosis of SP.


Assuntos
Peptídeos/sangue , Esquizofrenia/sangue , Adulto , Biomarcadores/sangue , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
3.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 21(1): 130-4, 2013 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-23484706

RESUMO

This study was purposed to establish a new quick and simple diagnostic method with high sensitivity and good specificity for idiopathic thrombocytopenic purpura (ITP) and to evaluate its significance. 240 platelet lysates (from patients with ITP, leukemia, MDS, and healthy adults, each of 60 cases) were randomly assigned to training set (120 cases) or validation set (120 cases), all of them were detected by surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS), in order to identify the differentially expressed protein, the diagnostic model was established by means of artificial neural network (ANN), and was validated by blind test with SPSS 17.0. The results showed that 5 marked proteins significantly differentially expressed (P < 0.01), m/z of highly expressed proteins were 2234.30, 3476.36, and 7526.29, m/z of low expressed proteins were 4990.02 and 5152.39, respectively. The sensitivity and specificity of diagnostic model were 80.6% and 77.3% respectively. The area under the ROC curve consisting of the output value of artificial neura1 network was 0.837. Efficacy of the model was validated by means of blinded test. It is concluded that the ANN model is useful for clinical diagnosis of ITP on the basis of platelet protein fingerprint spectrum.


Assuntos
Redes Neurais de Computação , Proteoma/análise , Púrpura Trombocitopênica Idiopática/diagnóstico , Adulto , Estudos de Casos e Controles , Humanos , Mapeamento de Peptídeos , Proteômica , Púrpura Trombocitopênica Idiopática/genética , Púrpura Trombocitopênica Idiopática/metabolismo , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
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