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1.
Food Chem ; 305: 125389, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31520918

RESUMO

A sensitive analytical method for the monitoring 2-methylimidazole and 4-methylimidazole (2-MI and 4-MI) is desirable due to their carcinogenic property. Here, we propose a highly sensitive method basing on the combination of solid-phase extraction and dispersive liquid-liquid microextraction techniques followed by high-performance liquid chromatography to simultaneously determine 2-MI and 4-MI in beverages. Dansyl chloride was used as a derivatizing reagent. Microextraction parameters were optimized by Plackett-Burman design and response surface methodology. Results show that derivatization led to significant improvements in chromatographic behavior for 2-MI and 4-MI due to increased hydrophobicity. The method shows good linearity (R2 ≥ 0.9985), satisfactory precision (%RSD ≤ 8.3%) and low limit of quantification (20 ng/mL), and was successfully applied to determine 2-MI and 4-MI in carbonated drinks, beers and energy drinks, achieving satisfactory recoveries (85-101%). This method provides a potential for routine analysis of 2-MI and 4-MI at the nanogram per milliliter level in beverages.

2.
J Colloid Interface Sci ; 559: 124-133, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31614317

RESUMO

Decorating electron-accepting materials on carbon nitride (C3N4) is a promising strategy to construct heterostructure catalysts for improved photocatalytic abilities. In this study, B-doped carbon-dots (B-C-dots) decorated C3N4 (C3N4/B-C-dots) catalysts were fabricated through the surface deposition. The benefits from integration of B-C-dots and C3N4 are four folds: (i) increasing surface area; (ii) improving visible light absorption; (iii) promoting the transfer of photoinduced carriers; and (iv) reducing the recombination of photoinduced carriers. The optimum photocatalytic activity of B-C-dots/C3N4 for Rhodamine B (Rh B) (or tetracycline hydrochloride (TC)) degradation was about 7.21 (6.56) and 4.80 (4.35) times higher than that of C3N4 and C-dots/C3N4, respectively, exhibiting both remarkable stability and repeatability. Moreover, enhanced photocatalytic activity of C3N4/B-C-dots could also be attributed to the type-II heterojunction formed between C3N4 and B-C-dots caused by B doping.

3.
J Med Virol ; 92(1): 53-61, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31429946

RESUMO

Hepatitis B virus (HBV) DNA is vulnerable to editing by human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (APOBEC) cytidine deaminases. However, the distribution of APOBEC-induced mutations on HBV DNA is not well characterized. To this end, we obtained the HBV DNA sequence of HBV-infected individuals with and without hepatocellular carcinoma (HCC and non-HCC groups, respectively) from NCBI database and calculated the rapo values of APOBEC-induced TpCpW→TpKpW mutation prevalence in HBV DNA. The results showed that the APOBEC-induced mutations were mainly distributed in the minus strand of non-HCC-derived HBV DNA (rapo = 2.04), while the mutation on the plus-strand was weaker (rapo = 0.99). There were high APOBEC-induced mutation regions in the minus strand of HBV DNA 1 to 1000 nucleotides (nts) region and in the plus-strand of HBV DNA 1000 to 1500 nts region; the mutations in the 1 to 1000 nts region were mainly TpCpW→TpTpW mutation types (total T/G: 111/18) and a number of these were missense mutations (missense/synonymous: 35/94 in P gene, 17/15 in S gene, and 5/10 in X gene). The difference between minus to plus-strand rapo of HCC-derived HBV DNA (1.96) was greater than that of the non-HCC group (1.05). The minus-strand rapo of HCC-derived HBV DNA regions 1000 to1500nts and 1500 to 2000 nts (rapo = 4.2 and 4.2) was also higher than that of the same regions of non-HCC-derived HBV DNA (rapo = 1.2 and 1.1). Finally, the ratio of minus to plus-strand rapo was used to distinguish HCC-derived HBV DNA from non-HCC-derived HBV DNA. This study unraveled the distribution characteristics of APOBEC-induced mutations on double strands of HBV DNA from HCC and non-HCC samples. Our findings would help understand the mechanism of APOBECs on HBV DNA and may provide important insights for the screening of HCC.

4.
Int J Hyg Environ Health ; 223(1): 106-115, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31606406

RESUMO

The application of Water Safety Plans (WSPs) in China varies throughout the country. Although pilot WSP projects in China were initiated shortly after WSP was introduced by the World Health Organization (WHO) in 2004, they have yet to be used for water supply facilities at a large scale. To better understand the evolution of WSP application in China, a systematic review was conducted to identify all published WSP related studies in China. Eighteen studies, which included 311 water systems, were included in the final analysis. Risk matrix, water supply risk factors, and other data were extracted and analyzed. Text mining methods were also used to better understand risks that can be addressed by WSPs (both potential and actual risks). This study revealed a number of noteworthy differences between and among urban and rural water systems in China. The primary risks associated with most urban water supply systems tended to be related to mechanical failure/s in the water treatment process. Rural water supply systems appear to suffer from similar problems, but insufficient overall management capacity was more prevalent in rural systems. Overall, the evidence suggests that, to date, the use of WSPs in China has been primarily limited to pilot studies, and full implementation of WSPs in China appears to still be in the early stages. The paper closes with a summary of the key obstacles identified as well as a discussion of policies and technical options which could increase the use of WSPs in both urban and rural China. Among other recommendations, the data indicate that there is strong need for the development and implementation of a simplified WSP approach designed specifically for small rural systems in China.

5.
J Nanosci Nanotechnol ; 20(2): 909-917, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383086

RESUMO

Tailored broussonetia-like NiCo2O4 is grown on carbon cloth using tri-sodium citrate assisted hydrothermal method. The chelating effect of citric ions has been utilized to investigate the morphological and structural evolution of NiCo2O4 on carbon cloth, which have been illustrated by scanning electron microscopy, transmission electron microscopy, and X-ray photoelectron spectra. The results demonstrate that the morphological alteration of NiCo2O4 from single nanowire to broussonetia-like structure has been detected after the addition of tri-sodium citrate. Citric ion plays a crucial role as an electrostatic stabilizer in determining this unique structure. When used as binder-free electrode in aqueous supercapacitors, the broussonetia-like NiCo2O4 electrode exhibits a specific capacitance of 527.9 F g-1 at a current density of 0.5 A g-1. Additionally, an asymmetric supercapacitor is further assembled using NiCo2O4 as the positive electrode and activated carbon as negative electrode. The device exhibits a maximum energy of 26.4 Wh kg-1 at power density of 800 W kg-1. A long-term cycling stability with 82% capacitance retention is maintained after 20,000 cycles at a current density of 5 A g-1, indicating the practical applicability of the tested device.

6.
J Nanosci Nanotechnol ; 20(4): 2292-2300, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492239

RESUMO

Fluorine-free superhydrophobic cotton fabric was fabricated by coating polyacrylate (PA)/SiO2 nanocomposite. PA/SiO2 nanocomposite was prepared based on the modified SiO2 nanoparticles with double bonds and hexadecyl groups by solution polymerization of butyl acrylate (BA), methyl methacrylate (MMA) and octadecylmethacrylate (OMA). The obtained cotton fabric showed excellent superhydrophobicity with a water contact angle of 152.2±0.4° and a water shedding angle of 8.0±0.2°, due to the simultaneous introduction of surface topography constructed by modified SiO2 nanoparticles and low surface free energy PA adhesive layer and hexadecyl groups onto cotton fibers. The as-obtained products were characterized by Fourier-transform infrared spectrometry (FT-IR), thermal gravimetric analysis (TG), scanning electron microscopy (SEM) and size distribution analysis. The obtained superhydrophobic fabric coated by PA/SiO2 demonstrated good mechanical stability and self-cleaning. The PA/SiO2 coating treatment caused little loss in the tensile strength, breathability, and whiteness of the treated fabric. This approach with improved human/environmental friendliness can pave the potential way for the preparation of superhydrophobic surfaces.

7.
Nanotechnology ; 31(5): 055705, 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-31618712

RESUMO

Cu-supported nitrogen-doped carbon nanofibers (NCNFs) were fabricated via electrospinning and subsequent activation treatment with poly vinylpyrrolidone as both carbon and nitrogen sources. The NCNFs are firmly adhered to Cu foil without any additional binder and form a hierarchical three-dimensional net structure, which could effectively shorten the diffusion paths for electrons and lithium ions, thus resulting in lower impedance and superior electrochemical properties. Additionally, NCNFs feature a amorphous carbon structure, N-rich carbon lattice and wide pore distribution, not only ensuring fast ions/electrons transport, but also giving rise to the higher energy density. When directly used as a binder-free electrode, NCNFs deliver a high reversible capacity of 617.8 mAh g-1 at 200 mA g-1 after 100 cycles and maintain a superior capacity of 274.1 mAh g-1 at 1.44 A g-1 even after 500 cycles. Besides, the reversible capacity up to 216.5 mAh g-1 can be still obtained at a high current density of 6 A g-1, demonstrating the excellent high-rate cyclability. The facile synthesis approach and superior electrochemical properties make NCNFs electrodes an alternative anode candidate for lithium-ion batteries.

8.
J Hazard Mater ; 383: 121129, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31546217

RESUMO

Antibiotics ingested in the human gut may create selective pressure to change the composition of the gut microbiota, which could adversely effect the immune system of the host. However, the occurrence and distribution of antibiotics in the human gut remains unclear. A total population of 180 individuals, across three Chinses regions with different economic development levels, including children, adults, and elders, were sampled in 2017. A total of 19 representative antibiotics, including both clinical and veterinary antibiotics, were investigated in human faeces. While clinical use and prescriptions were the main exposure pathways for children, environmental media were the exposure pathway to adults. In addition, significant differences (P < 0.05) in antibiotic residues in human faeces were observed amongst various economic development levels, where human faeces from underdeveloped areas were mostly associated with higher levels of antibiotics. This study first to investigate the occurrence and distribution of typical antibiotics in the faeces of a Chinese population and thereby provide a reference for the intensive study of the effects and mechanisms of antibiotics on human gut microbiota.

9.
J Appl Microbiol ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31671233

RESUMO

AIMS: This study was conducted to assess the effects of acute heat stress (HS) on intestinal microbiota, and the associations with the changes in feed intake (FI) and serum profile. METHODS AND RESULTS: Twenty four individually housed pigs (Duroc × Large White × Landrace, 30 ± 1 kg body weight) were randomly assigned to receive 1 of 3 treatments (8 pigs / treatment): 1) thermal neutral (TN) conditions (25 ± 1°C), 2) HS conditions (35 ± 1°C), 3) pair-feeding (PF) with HS under TN conditions. After 24-h treatment, pigs were monitored to assess feed intake (FI), and samples of serum and feces were collected to investigate serum profile, microbial composition, and short chain fatty acids (SCFAs). The results showed that HS decreased (P < 0.05) FI compared with the TN group. Compared with TN group, HS changed serum profile by affecting biochemical parameters and hormones related with energy metabolism and stress response; immune indicators were also altered in HS group. Most of changes in serum profile were independent of FI reduction. Additionally, HS shifted the diversity and composition of fecal microbial community by increasing (P < 0.05) Proteobacteria and decreasing (P < 0.05) Bacteroidetes. Moreover, HS decreased (P < 0.05) the concentrations of propionate, butyrate, valerate, iso-valerate, and total SCFAs in feces with a FI-independent manner. Furthermore, the Spearman correlation analysis implied that changes of serum profile have potential correlation with alterations of fecal microbiota and their metabolites SCFAs in acute HS-treated grow-finishing pigs. CONCLUSIONS: Metabolism disorders caused by 24-h acute HS associated with changes of fecal microbiota and their metabolites SCFAs with a FI-independent manner in grow-finishing pigs. SIGNIFICANCE AND IMPACT OF THE STUDY: These results give us a new insight of the intestinal damage caused by acute HS and the underlying mechanisms.

10.
Cancer Res Treat ; 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31671936

RESUMO

Purpose: To investigate the prognostic impact of EBV-miR-BHRF1-1 with chronic lymphocytic leukemia (CLL) as well as role of EBV-miR-BHRF1-1 in p53 gene. Materials and Methods: Quantitative reverse transcription-PCR (qRT-PCR) and western blotting (WB) were used to quantify EBV-miR-BHRF1-1 and p53 expression in cultured CLL. Results: p53 aberration was associated with higher expression level of Epstein-Barr virus (EBV)-microRNA (miRNA, miR)-BHRF1-1 (p<0.001) which was also an independent prognostic marker for overall survival (OS) (p=0.028; HR 5.335 [1.193, 23.846]) in 97 newly-diagnosed CLL patients after adjusted with CLL-international prognostic index (CLL-IPI). We identified EBV-miR-BHRF1-1 as a viral miRNA regulator of p53. EBV-miR-BHRF1-1 repressed luciferase reporter activity by specific interaction with the seed region within the p53 3' untranslated region. Discordance of p53 messenger RNA and protein expression was associated with high EBV-miR-BHRF1-1 levels in CLL patients and cell lines. EBV-miR-BHRF1-1 inhibition upregulated p53 protein expression, induced cell cycle arrest and apoptosis and decreased cell proliferation in cell lines. EBV-miR-BHRF1-1 mimics downregulated p53 protein expression, decreased cell cycle arrest and apoptosis, and induced cell proliferation in cell lines. Conclusion: This study supported a role of EBV-miR-BHRF1-1 in p53 regulation in vitro. Our results support the potential of EBV-miR-BHRF1-1 as a therapeutic target in EBV-associated CLL with p53 gene aberration.

11.
Appl Opt ; 58(27): 7375-7378, 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31674386

RESUMO

In this paper, an integrated processing method was demonstrated to fabricate the polymer-based thermo-optic (TO) switch with low power consumption. The characteristic parameters of the switch were carefully designed and simulated. The air trench structure was exploited to reduce the power consumption, which can be formed with the waveguide simultaneously by the integrated processing method. Moreover, the introduced polymer/silica hybrid waveguide structure can also improve the response time of the device. A typical fabricated switch presented a low switching power of 5.2 mW. The measured switching rise time and fall time are 192.2 and 201.1 µs, respectively.

12.
Int J Cancer ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31693169

RESUMO

The heterogeneities of colorectal cancer (CRC) lead to staging inadequately of patients' prognosis. Here, we performed a prognostic analysis based on the tumor mutational profile and explored the characteristics of the high-risk tumors. We sequenced 338 colorectal carcinomas as the training dataset, constructed a novel five-gene (SMAD4, MUC16, COL6A3, FLG and LRP1B) prognostic signature, and validated it in an independent dataset from The Cancer Genome Atlas (TCGA). Kaplan-Meier and Cox regression analyses confirmed that the five-gene signature is an independent predictor of recurrence and prognosis in patients with Stage III colon cancer. The mutant signature translated to an increased risk of death (hazard ratio = 2.45, 95% confidence interval = 1.15-5.22, p = 0.016 in our dataset; hazard ratio = 4.78, 95% confidence interval = 1.33-17.16, p = 0.008 in TCGA dataset). RNA and bacterial 16S rRNA sequencing of high-risk tumors indicated that mutations of the five-gene signature may lead to intestinal barrier integrity, translocation of gut bacteria and deregulation of immune response and extracellular related genes. The high-risk tumors overexpressed IL23A and IL1RN genes and enriched with cancer-related bacteria (Bacteroides fragilis,Peptostreptococcus, Parvimonas, Alloprevotella and Gemella) compared to the low-risk tumors. The signature identified the high-risk group characterized by gut bacterial translocation and upregulation of interleukins of the tumor microenvironment, which was worth further researching.

13.
Amino Acids ; 51(10-12): 1547-1559, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31720834

RESUMO

Mammary gland development during late pregnancy in sows is a major factor affecting the composition of colostrum and milk and the pre-weaning growth of piglets, while valine is essential for protein and nitrogen metabolism in mammary gland of sow. However, the effects of valine and its underlying mechanism on mammary gland development during late pregnancy are still unclear. Here, we hypothesized that dosage of dietary valine during late pregnancy will affect protein synthesis of colostrum in gilts. The results showed that supplementation of valine during late pregnancy significantly increased content of protein (P < 0.01), fat (P = 0.02) and solids-non-fat (P = 0.04) in colostrum. Our in vitro study also confirmed that valine supplementation increased protein synthesis and cell proliferation in porcine mammary epithelial cells (PMEC). Furthermore, these changes were associated with elevated phosphorylation levels of mammalian target of rapamycin (mTOR), and ribosomal protein S6 kinase (S6) and eukaryotic initiation factor 4E-binding protein-1 (4EBP1) in valine-supplemented cells, which could be effectively blocked by the antagonists of mTOR. These findings indicated that valine enhanced mammary gland development and protein synthesis in colostrum via the mTOR signaling pathway. These results, using an in vivo and in vitro model, helped to understand the beneficial effects of dietary valine supplementation on gilts.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31722524

RESUMO

The vertical composition distribution of a bulk heterojunction (BHJ) photoactive layer is known to have dramatic effects on photovoltaic performance in polymer solar cells. However, the vertical composition distribution evolution rules of BHJ films are still elusive. In this contribution, three BHJ film systems, composed of polymer donor PBDB-T, and three different classes of acceptor (fullerene acceptor PCBM, small-molecule acceptor ITIC, and polymer acceptor N2200) are systematically investigated using neutron reflectometry to examine how donor-acceptor interaction and solvent additive impact the vertical composition distribution. Our results show that those three BHJ films possess homogeneous vertical composition distributions across the bulk of the film, while very different composition accumulations near the top and bottom surface were observed, which could be attributed to different repulsion, miscibility, and phase separation between the donor and acceptor components as approved by the measurement of the donor-acceptor Flory-Huggins interaction parameter χ. Moreover, the solvent additive 1,8-diiodooctane (DIO) can induce more distinct vertical composition distribution especially in nonfullerene acceptor-based BHJ films. Thus, higher power conversion efficiencies were achieved in inverted solar cells because of facilitated charge transport in the active layer, improved carrier collection at electrodes, and suppressed charge recombination in BHJ solar cells.

15.
Acta Trop ; 202: 105253, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31678234

RESUMO

This study aimed to investigate the effects of programmed cell death protein 10 (PCDP10) on the female reproductive system of Schistosoma japonicum, one of the major infectious agents of schistosomiasis. We found that PCDP10 was widely distributed in the integument, the worm parenchymal area, and the vitellarium of the female worm, but was localized to a lesser extent in the ovary and testicles. RNAi experiments successfully achieved gene knockdown, and the ultrastructural morphology of the adult reproductive organs was observed. The results demonstrated that, compared with those of the negative control group, the number of cortical granules around oocytes decreased and the number of immature oocyte cells increased. Fusion of yolk globules occurred, and the number and the diameter of yolk droplets decreased significantly. Real-time PCR showed that the expression of yolk glands reached its peak before ovulation and then decreased. The TUNEL assay results showed that apoptosis in the RNAi group was significantly higher than that in the negative control group. These results suggested that SjPCDP10 plays an important role in the female reproductive system. In conclusion, PCD10 is involved in oocyte growth and development, especially in eggshell formation, which may provide a reference for further elucidating the molecular mechanism of PCDP10 involved in egg formation and embryo development in Schistosoma japonicum.

16.
ACS Appl Mater Interfaces ; 11(47): 44582-44592, 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31682097

RESUMO

Janus nanoparticles with an anisotropic feature concentrated multiple properties on a single carrier, providing synergistic effects. In this study, dual-functionalized Janus nanoparticles (HA-JMSN/DOX-DMMA) were constructed with a tumor-targeting ligand (hyaluronic acid, HA) modified on the one side and a charge reversal group (2,3-dimethylmaleic anhydride, DMMA) on the other side. The drug release of HA-JMSN/DOX-DMMA was positively correlated with the acidity of the environment. The cytotoxicity and cell uptake of HA-JMSN/DOX-DMMA were superior to the isotropous nanoparticles. The endocytosis pathway of HA-JMSN/DOX-DMMA involved the clathrin-mediated endocytosis (HA) and the micropinocytosis (DMMA) at the same time, which indicated that they both participated in the interaction between nanoparticles and tumor cells. After being injected intravenously in mice, the distribution of HA-JMSN/DOX-DMMA in tumor was enhanced significantly. The antitumor therapy study in vivo showed that HA-JMSN/DOX-DMMA inhibited tumor growth and improved the survival rate of tumor-bearing mice effectively. In general, HA-JMSN/DOX-DMMA could take the synergistic effect of active targeting and charge reversal to deliver drug in tumor cells and kill them efficiently, which was a promising antitumor nanodrug.

17.
J Med Chem ; 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31730343

RESUMO

MEK1 and MEK2 (also known as MAP2K1 and MAP2K2) are the "gatekeepers" of the ERK signaling output with redundant roles in controlling ERK activity. Numerous inhibitors targeting MEK1/2 have been developed including three FDA-approved drugs. However, acquired resistance to MEK1/2 inhibitors has been observed in patients, and new therapeutic strategies are needed to overcome the resistance. Here, we report a first-in-class degrader of MEK1/2, MS432 (23), which potently and selectively degraded MEK1 and MEK2 in a VHL E3 ligase- and proteasome-dependent manner and suppressed ERK phosphorylation in cells. It inhibited colorectal cancer and melanoma cell proliferation much more effectively than its negative control MS432N (24), and its effect was phenocopied by MEK1/2 knockdown. Compound 23 was highly selective for MEK1/2 in global proteomic profiling studies. It was also bioavailable in mice and can be used for in vivo efficacy studies. We provide two well-characterized chemical tools to the biomedical community.

18.
Nanoscale ; 11(46): 22440-22445, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31746893

RESUMO

High-throughput growth of large size transition metal dichalcogenide (TMD) single crystals is an important challenge for their applications in the next generation electronic and optoelectronic integration devices. Here we report the high-throughput growth of submillimeter monolayer TMD single crystals by two-stage space confined chemical vapor deposition, where the nucleation density of TMD crystals is significantly decreased for the growth of large size monolayer crystals by the space confinement effect. Moreover, high-throughput growth of submillimeter TMD crystals is also achieved by stacking the substrates along the perpendicular direction to the flow of the reaction gases. The mobilities of the TMD materials produced in this way are up to 1.2, 17.0 and 25.0 cm2 (V s)-1 for monolayer WS2, WSe2 and MoS2 single crystals, respectively. The results demonstrate that two-stage space confined growth is a highly promising method for high-throughput fabrication of high-quality submillimeter monolayer TMD single crystals, which will pave a new pathway to large-scale production of TMD-based electronic and optoelectronic devices.

19.
J Inorg Biochem ; 203: 110914, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31751818

RESUMO

α-Glucosidase is an important target enzyme for the treatment of type 2 diabetes in humans. In our previous studies, it was found that polyoxometalates exhibited an effective inhibitory effect on the activity of α-glucosidase, while polyoxometalates have the characteristics of structural diversity and unique properties. Herein, we investigated the inhibition of two different series of polyoxometalates on α-glucosidases by enzyme kinetics and molecular docking. The results demonstrated that all of the studied compounds had a significant inhibitory ability on α-glucosidase as compared with the positive control acarbose. H8[P2Mo17Cr(OH2)O61] reversibly inhibited α-glucosidase in a competitive manner with IC50 of 115.50 ±â€¯1.64 µM and KI value of 44.31 µM. All other compounds reversibly inhibited enzymatic activity in a mixed manner. H6PMo9V3O40 and H8[P2Mo17Cu(OH2)O61] were the best inhibitors in the Keggin and Dawson series, respectively, with IC50 of 9.63 ±â€¯0.43 and 40.13 ±â€¯0.61 µM, respectively. We conducted molecular docking study and found that the compound and α-glucosidase were mainly non-covalently interacting with hydrogen bonds and van der Waals forces. This result further confirmed the inhibition mechanism of enzyme kinetic experiments.

20.
Anxiety Stress Coping ; : 1-8, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31771350

RESUMO

Background and Objectives: Post-traumatic stress disorder (PTSD) and major depressive disorder are commonly experienced mental disorders among psychological trauma victims. Few studies have investigated the genetic basis for population heterogeneity of trauma-related psychopathology, including PTSD and depression. This study examined the main and interaction effects of the OXTR rs53576 genotype in distinguishing four subgroups identified by symptom profiles of PTSD and depression symptoms using latent profile analysis.Design: A cross-sectional design with a gene-environment interaction approach was adopted in the current study.Methods: This study was a secondary data analysis conducted on a sample of 1196 adult earthquake survivors. Participants completed assessments of earthquake exposure, PTSD symptoms, and depression symptoms. The rs53576 polymorphism of OXTR was genotyped using a custom-by-design 2×48-Plex SNPscanTMKit.Results: Multinomial logistic regression analyses revealed the main effects of the rs53576 genotype on symptom profiles. Specifically, G allele carriers were more likely in the combined PTSD-depression group than in the low symptom, predominantly depression, and predominantly PTSD groups. No significant interaction effects between this genotype and earthquake exposure on symptom profiles were found.Conclusions: Our findings support a genetic basis for trauma-related psychopathology heterogeneity. Furthermore, results provide preliminary evidence for the role of OXTR in PTSD/depression comorbidity.

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