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1.
Pest Manag Sci ; 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34596946

RESUMO

BACKGROUND: The brown planthopper (BPH), Nilaparvata lugens (Stål), is the most severe pest attacking rice crops with sucking mouthparts. It causes significant damages to rice growth and food production worldwide. With the long-term and wide use of insecticides, the field populations of BPH have developed resistance to many insecticides. RESULTS: Here, we showed that up-regulation of an ATP-binding cassette transporter gene NlMdr49-like contributes to imidacloprid resistance in the field populations of BPH. A comparative transcriptome analysis was performed to evaluate the gene expression in two field populations (JXSG18 and YNTC18). Compared with susceptible strain (Sus), 202 up-regulated genes and 170 down-regulated genes were identified in both field populations. Functional enrichment analysis revealed that the differentially expressed genes (DEGs) are mainly linked to metabolic process and transmembrane transport. Among the candidate DEGs, NlMdr49-like was significantly up-regulated in both field populations. Based on the genome and transcriptome of BPH, the full-length cDNA of NlMdr49-like was sequenced and its molecular characteristics were analyzed. The expression pattern analysis of various tissues showed that NlMdr49-like was predominantly expressed in midgut and Malpighian tubules which are important excretion organs. Knocking down NlMdr49-like reduced BPH resistance to imidacloprid, but did not affect its resistance to the other nine insecticides (chlorpyrifos, thiamethoxam, nitenpyram, dinotefuran, sulfoxaflor, triflumezopyrim, ethiprole, buprofezin and pymetrozine). Furthermore, transgenic strain of Drosophila melanogaster overexpressing NlMdr49-like was less susceptible to imidacloprid. CONCLUSIONS: Our findings indicate that up-regulation of NlMdr49-like is another mechanism contributing to imidacloprid resistance in N. lugens. And this result is helpful to further understand the resistance mechanism of N. lugens to imidacloprid. This article is protected by copyright. All rights reserved.

2.
Zhongguo Zhong Yao Za Zhi ; 46(12): 3016-3023, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34467691

RESUMO

The acupoint application of Euodiae Fructus at Yongquan(KI1) can significantly improve the sleep quality of patients with insomnia with berberine as the main effective component for the efficacy. Nineteen active compounds and 203 drug targets were screened out from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). After comparison with GeneCards and Online Mendelian Inheritance in Man(OMIM), 24 common genes of diseases and drugs were obtained. STRING 11.0 was used to construct a protein-protein interaction(PPI) network of the overlapping genes, and Matthews correlation coefficient(MCC) was employed to screen the core genes, which were then subjected to enrichment analysis with gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG). The results revealed that the main compounds of Euodiae Fructus, such as berberine and rutaecarpine, participated in the biological processes(such as neurotransmitter receptor activity) by regulating C-reactive protein(CRP), estrogen receptor 1(ESR1), 5-hydroxytryptamine(5-HT) receptor, and interleukin-6(IL-6) to exert sedative, anxiolytic, and antidepressant effects. Sixty 4-week-old SPF mice were randomly divided into a control group, a model group, a positive drug(diazepam tablets) group, and low-, medium-, and high-dose berberine groups. Medication with corresponding drugs was performed for one week. The results demonstrated that berberine was potent in reducing the activities and standing times of mice, down-regulating the levels of CRP and IL-6 mRNA in the hypothalamus, and up-regulating the expression of 5-HT(P<0.01); however, no significant effect on ESR1 was observed. The network of Euodiae Fructus in treating insomnia was constructed by network pharmacology and verified by tests. The findings indicated that the therapeutic efficacy of Euodiae Fructus in treating insomnia was achieved by participating in multiple biological processes, such as neurotransmitter receptor activity, which provided a scientific basis for its clinical application.


Assuntos
Medicamentos de Ervas Chinesas , Distúrbios do Início e da Manutenção do Sono , Animais , Bases de Dados Genéticas , Medicamentos de Ervas Chinesas/farmacologia , Ontologia Genética , Humanos , Medicina Tradicional Chinesa , Camundongos , Distúrbios do Início e da Manutenção do Sono/tratamento farmacológico , Distúrbios do Início e da Manutenção do Sono/genética
3.
Int J Med Sci ; 18(11): 2449-2456, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33967623

RESUMO

Parkinson protein 7 (PARK7)/DJ-1 (DJ-1) is a redox sensitive molecular and stabilizer of nuclear factor erythroid 2-related factor 2 (Nrf-2). Nrf-2 regulates the downstream antioxidant defense system and exerts a significant function in patients with chronic obstructive pulmonary disease (COPD). Vitamin D receptor (VDR) is the nuclear receptor that regulates the downstream target genes. This study aimed to analyze the associations among pulmonary function, DJ-1, VDR and Nrf-2 in COPD patients. Serum was collected from 180 COPD patients and control subjects. Thirty-five lung tissues were obtained. DJ-1 was measured using ELISA and western blotting. Nrf-2 and VDR were detected by immunohistochemistry. Serum and pulmonary DJ-1 levels were lower in COPD patients than those in control subjects. Pulmonary VDR-positive nuclei were reduced in COPD patients. Nrf-2-positive nuclei were reduced in lung tissues of COPD patients. On the contrary, Nrf-2-related downstream target proteins were elevated in COPD patients. Further correlation analysis indicated that forced expiratory volume in 1 second (FEV1) was positively associated with pulmonary DJ-1, VDR and Nrf-2 in patients with COPD. In addition, there were positive correlations among DJ-1, VDR and Nrf-2 in lung tissues of COPD patients. In conclusion, DJ-1, VDR and Nrf-2 were decreased in COPD patients compared with control subjects. The reduction of DJ-1 and VDR associating with Nrf-2 downregulation may be involved in the process of COPD.

4.
Pest Manag Sci ; 77(9): 4128-4137, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33913602

RESUMO

BACKGROUND: Pymetrozine is commonly used for the control of Nilaparvata lugens, and resistance to pymetrozine has been frequently reported in the field populations in recent years. However, the mechanism of brown planthopper resistance to pymetrozine is still unknown. RESULTS: In this study, a pymetrozine-resistant strain (PMR) was established, and the potential biochemical resistance mechanism of N. lugens to pymetrozine was investigated. Pymetrozine was synergized by the inhibitor piperonyl butoxide (PBO) in the PMR with 2.83-fold relative synergistic ratios compared with the susceptible strain (Sus). Compared with the Sus, the cytochrome P450 monooxygenase activity of PMR was increased by 1.7 times, and two P450 genes (NlCYP6CS1 and NlCYP301B1) were found to be significantly overexpressed more than 6.0-fold in the PMR. Pymetrozine exposure induced upregulation of NlCYP6CS1 expression in the Sus, but the expression of NlCYP301B1 did not change significantly. In addition, RNA interference (RNAi)-mediated suppression of NlCYP6CS1 gene expression dramatically increased the toxicity of pymetrozine against N. lugens. Moreover, transgenic lines of Drosophila melanogaster expressing NlCYP6CS1 were less susceptible to pymetrozine, and had a stronger ability to metabolize pymetrozine. CONCLUSIONS: Taken together, our findings indicate that the overexpression of NlCYP6CS1 is one of the key factors contributing to pymetrozine resistance in N. lugens. And this result is helpful in proposing a management strategy for pymetrozine resistance.


Assuntos
Hemípteros , Inseticidas , Animais , Sistema Enzimático do Citocromo P-450/genética , Drosophila melanogaster , Hemípteros/genética , Resistência a Inseticidas/genética , Inseticidas/farmacologia , Neonicotinoides , Nitrocompostos , Triazinas
5.
Ecotoxicol Environ Saf ; 215: 112164, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33756289

RESUMO

Environmental cadmium (Cd) exposure can cause several pulmonary diseases. Epithelial-mesenchymal transition (EMT) involved in the process of chronic obstructive pulmonary disease (COPD). However, the association between environmental Cd exposure and EMT was unclear in COPD patients. This study aimed to analyze the associations among circulatory Cd, EMT and COPD based on case-control study. Four hundred COPD patients and 400 control subjects were recruited. Circulatory Cd was detected using atomic adsorption spectrometer. MicroRNA-30 (miR-30) was measured by RT-PCR and the markers of pulmonary EMT were evaluated through western blotting. Circulatory Cd concentration was increased and serum miR-30 was decreased in COPD patients. Circulatory Cd was inversely associated with pulmonary function in COPD patients. Moreover, serum miR-30 was gradually decreased in parallel with FEV1 in COPD patients. Meanwhile, there was a negative association between serum miR-30 and circulatory Cd in COPD patients. Further analysis found that E-cadherin, one of epithelial biomarkers, was reduced in lung tissues of COPD patients with higher circulatory Cd. On the contrary, pulmonary N-cadherin, Vimentin and α-SMA, three of mesenchymal biomarkers, were increased in COPD patients with higher circulatory Cd. In vitro experiments revealed that Cd exposure repressed miR-30 levels and promoted EMT in BEAS-2B cells. Our results provide evidence that miR-30 reduction contributing to pulmonary EMT may involve in the process of Cd-induced COPD.


Assuntos
Cádmio/sangue , Exposição Ambiental/estatística & dados numéricos , Poluentes Ambientais/sangue , Doença Pulmonar Obstrutiva Crônica/sangue , Antígenos CD , Caderinas , Estudos de Casos e Controles , Transição Epitelial-Mesenquimal , Feminino , Humanos , Pulmão/fisiopatologia , Masculino , MicroRNAs , Pessoa de Meia-Idade , Doença Pulmonar Obstrutiva Crônica/fisiopatologia , Vimentina
6.
Pestic Biochem Physiol ; 173: 104780, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33771259

RESUMO

Transient receptor potential (TRP) is a superfamily of important cation channels located on the cell membrane. It can regulate almost all sensory modality and control a series of behaviors, including hearing, locomotion, gentle touch, temperature sensation, dry air and food texture detection. The expression profiles of TRP channels have been well documented in the model insect Drosophila melanogaster. However, little is known about the TRP channels of agricultural pests. In this study, we cloned 9 TRP ion channel genes from brown planthopper. Their amino acid sequences are highly conserved with homologues of other insects and have typical TRP channel characteristics: six transmembrane domains (TM1 - TM6) and a pore region between TM5 and TM6. These TRP channels of N. lugens were expressed in all developmental stages and various body parts. The expression levels of almost all TRP channels were relatively higher in adults than nymph stages, and lowest in the eggs. Antenna and abdomen were the main body parts with high expression of these genes. Furthermore, the mRNA levels of these TRP genes were significantly decreased in the third-instar nymphs injected with double-stranded RNA (dsRNA). The survival rate of different TRP dsRNA injected nymphs all exceeded 81%, which was no significant difference compared with the control group. These results suggested that these 9 TRP channels are expressed throughout the body and all ages of the brown planthopper, and are involved in regulating multiple physiological and behavioral processes. The identification of TRP channel genes in this study not only provides a foundation for further exploring the potential roles of TRP channels, but also serves as targets to develop new insecticides for the control of agricultural pests.


Assuntos
Hemípteros , Canais de Potencial de Receptor Transitório , Animais , Drosophila melanogaster/metabolismo , Hemípteros/genética , Hemípteros/metabolismo , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Ninfa/metabolismo , RNA de Cadeia Dupla/genética , Canais de Potencial de Receptor Transitório/genética
7.
Pestic Biochem Physiol ; 165: 104548, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32359557

RESUMO

Pymetrozine is a promising chemical used to control brown planthopper, which developed resistance to imidacloprid and buprofezin in the past decade. Field efficacy indicates that pymetrozine can reduce the number of offsprings of brown planthopper, but the specific physiological mechanism is unknown. In this study, we systematically described the mating process of brown planthopper including 8 steps (abdominal vibration, following, positioning, wing extension, attempted copulation, copulation, terminated copulation and leaving) and explored the optimal mating time after adult eclosion (3-5 days) and observation time (30 mins). Also, behavioral data showed that pymetrozine can affect the mating behavior and female fecundity of brown planthopper and fruit fly. As one of the target genes for pymetrozine, Nanchung (Nan), the nan36a mutant male courtship index, female receptivity and the number of offsprings were significantly decreased. Behavioral defects in nan36a mutant flies can be rescued by expressed NlNan. Our results indicated that Nan plays essential roles in the mating behavior and female fecundity. These findings provide useful information for demonstrating that pymetrozine effectively reduce the reproduction of brown planthopper and contribute to our understanding of reproductive strategies controlled by pymetrozine in insects.


Assuntos
Hemípteros , Comportamento Reprodutivo , Animais , Proteínas de Drosophila , Drosophila melanogaster , Feminino , Masculino , Canais de Potencial de Receptor Transitório , Triazinas
8.
Zhongguo Gu Shang ; 32(8): 742-745, 2019 Aug 25.
Artigo em Chinês | MEDLINE | ID: mdl-31533387

RESUMO

OBJECTIVE: To explore clinical effect of VSD technology, coverage of artificial dermis and autograft for the treatment of limb skin soft tissue defect combined with bone or tendon exposed wound. METHODS: Eighteen patients suffered from limb skin soft tissue defect combined with bone or tendon exposed wound treated by three-step sequential method from January 2013 to June 2015. Among them, including 13 males and 5 females aged from 23 to 72 years old with an average of 34.6 years old; the time from injury to operation ranged from 1.5 to 5.0 hours with an average of 2.5 h. The area of skin and soft tissue injury ranged from 4.2 cm×3.1 cm to 7.4 cm×5.2 cm. Wound recovery and taken skin wound recovery were observed to evaluate clinical results. RESULTS: All patients were followed up from 5 to 16 months, with an average of 7.6 months. Deep bone tendon tissue of wounds were effectively recovered, artificial dermis survived, and quality of healed wound was tough and shape was good. Wound transplant flap was survived, no obvious scar tissue formation, appearance was flat, skin color was a little deeper than normal skin, the overall effect was satisfactory. CONCLUSIONS: Three-step sequential method has good curative effect for patients suffered from limb skin soft tissue defect with bone or tendon exposed wound and refused to repair the flap, and has advantage of simple operation, operation risk, less invasive.


Assuntos
Procedimentos Cirúrgicos Reconstrutivos , Lesões dos Tecidos Moles , Tendões , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Transplante de Pele , Retalhos Cirúrgicos , Resultado do Tratamento , Adulto Jovem
10.
Zhongguo Gu Shang ; 32(1): 56-59, 2019 Jan 25.
Artigo em Chinês | MEDLINE | ID: mdl-30813670

RESUMO

OBJECTIVE: To explore surgical methods and clinical effects of three different types of mini skin flap transplantation for repairing finger soft tissue with bone defect. METHODS: Thirty-three patients with finger soft tissue or bone defect were treated from December 2014 to October 2016, including 24 males and 9 females aged from 21 to 52 years old with an average of (36.42±5.70) years old, and soft tissue defect area ranged from 1.3 cm×1.8 cm to 2.3 cm×4.2 cm. According to damage degree, nature and patients' options, 15 finger of 15 cases were adopted retrograde dorsal metacarpal artery perforators fascia flap, 10 fingers of 9 cases were treated with free foot artery descending branch wrist skin flap, 9 fingers of 9 cases were treated with free the second toe details phalanges compound flap. Survival rate, postoperative complications and finger function assessed by Dargan functional criteria at the latest follow up were observed. RESULTS: All flaps were survived, both of donor site and recipient site were without deep infected. The donor site of one patient occurred necrotic, and the distal donor site of one patient occurred surface necrotic, then healed by active dressing change. All patients were followed up from 6 to 16 months with an average of(8.34±1.28) months. Two points of finger recognition were restored between 8 and 12 mm with an average of (8.84±0.43) mm, and the appearance, texture and sensory functions of skin flap were restored. No obvious complications were observed on the donor site. According to Dargan function evaluation of finger joints, 18 patients got excellent results, 14 moderate and 1 good. CONCLUSIONS: Three kinds of mini skin flap could receive good results in repairing soft tissue of finger or bone defect. Reverse dorsal metacarpal artery perforator fascia flap is not necessary with anastomosing blood vessels and has advantages of safe, simple and high survival rate. Descending branch of superior cutaneous branch of free ulnar artery could cut multiple other perforator flaps simultaneously, and the scar is small and hidden. Dissociated the second toe combined metatarsal phalangeal flap could repair shape and function of finger to the maximum extent and donor site is hidden.


Assuntos
Traumatismos dos Dedos , Procedimentos Cirúrgicos Reconstrutivos , Lesões dos Tecidos Moles , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pele , Transplante de Pele , Resultado do Tratamento , Adulto Jovem
11.
Biol Trace Elem Res ; 192(2): 263-276, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30790121

RESUMO

Selenium (Se), as a nutritionally essential trace element, has been shown to decrease with age and is closely related to Alzheimer's disease (AD). To probe the effects of Se on AD pathology, two-dimensional fluorescence difference gel electrophoresis was applied to the serum samples collected from the wild-type (WT) mice and the triple transgenic (PS1M146V/AßPPSwe/TauP301L) AD mice (3xTg-AD), treated with or without sodium selenate in drinking water for 4 months beginning at 2 months of age. Proteomics results revealed 17 differentially expressed proteins between WT and 3xTg-AD mice. It was found that the administration of selenate reversed the alterations of the differentially expressed serum proteins by up-regulating 13 proteins and down-regulating 2 proteins which were reported to be involved in the key pathogenesis of AD, including regulation of Aß production, lipid metabolism regulation, and anti-inflammation. These results suggested that a dietary supplement with selenate is effective for prevention and treatment of AD, and the mechanism was maybe related to its role in Aß regulation, lipid metabolism, and anti-inflammation. Moreover, we also presented that α-2 macroglobulin, transthyretin, haptoglobin, alpha-2-HS-glycoprotein, and alpha-1-antitrypsin in the serum can be used to evaluate the effect of selenate on AD pathology.


Assuntos
Doença de Alzheimer/tratamento farmacológico , Modelos Animais de Doenças , Proteômica , Ácido Selênico/farmacologia , Doença de Alzheimer/sangue , Doença de Alzheimer/patologia , Animais , Glicoproteínas/antagonistas & inibidores , Glicoproteínas/sangue , Haptoglobinas/análise , Haptoglobinas/antagonistas & inibidores , Camundongos , Camundongos Endogâmicos , Camundongos Transgênicos , Pré-Albumina/análise , Pré-Albumina/antagonistas & inibidores , alfa 2-Macroglobulinas Associadas à Gravidez/análise , alfa 2-Macroglobulinas Associadas à Gravidez/antagonistas & inibidores , alfa 1-Antitripsina/sangue , alfa 1-Antitripsina/metabolismo
12.
Pestic Biochem Physiol ; 153: 77-86, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30744899

RESUMO

The commercial insecticide pymetrozine has been extensively used for brown planthopper control in East Asia. The transient receptor potential vanilloid (TRPV) channel, which consists of two proteins, Nanchung (Nan) and Inactive (Iav), has recently been shown to be the molecular target of pymetrozine in the fruit fly (Drosophila melanogaster) and pea aphid (Acyrthosiphon pisum). In this study, we characterized the Nan and Iav TRPV channel subunits of N. lugens and measured the action of pymetrozine on them. NlNan and NlIav are structurally similar to homologs from other insects. The expression pattern analysis of various body parts showed that NlNan and NlIav were both more abundantly expressed in antennae. When NlNan and NlIav were co-expressed in Xenopus laevis oocytes, they formed channels with high sensitivity to pymetrozine (EC50 = 5.5 × 10-8 M). Behavioral observation revealed that the gravitaxis defect in the fruit fly nan36a mutant was rescued by ectopically expressed NlNan and the rescued behavior could be abolished by pymetrozine. Our results confirm that NlNan and NlIav co-expressed complexes can be activated by pymetrozine both in vitro and in vivo and provide useful information for future resistance mechanism studies.


Assuntos
Hemípteros/efeitos dos fármacos , Proteínas de Insetos/fisiologia , Inseticidas/toxicidade , Canais de Potencial de Receptor Transitório/fisiologia , Triazinas/toxicidade , Animais , Animais Geneticamente Modificados , Comportamento Animal/efeitos dos fármacos , Drosophila melanogaster/genética , Feminino , Hemípteros/fisiologia , Masculino , Oócitos , Xenopus
13.
Insect Biochem Mol Biol ; 106: 55-63, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30496804

RESUMO

NompC channel is a member of the transient receptor potential (TRP) ion channel superfamily. It can regulate gentle-touch, locomotion, hearing and food texture detection in Drosophila. We cloned the NompC gene of Nilaparvata lugens (NlNompC). The full length NlNompC possessed similar structure as DmNompC, which belongs to TRPN subfamily. The expression pattern analysis of different developmental stages and body parts showed that the transcription of NlNompC was more abundant in adult stage and in the abdomen. Injection of double-stranded RNA (dsRNA) of NlNompC in the third-instar nymphs successfully knocked down the target gene with 75% suppression. At nine days after injection, the survival rate of dsRNA injected nymphs was as low as 9.84%. Behavioral observation revealed that the locomotion of the dsRNA injected nymphs was defective with much less movement compared to the negative control. Feeding and honeydew excretion of the dsRNA injected insects also decreased significantly. These results suggested that NlNompC is a classical mechanotransduction channel that plays important roles in proprioception and locomotion, and is essential for the survival of N. lugens. The results also contribute to the understanding of how TRP channels regulate proprioception.


Assuntos
Hemípteros/fisiologia , Proteínas de Insetos/genética , Propriocepção/genética , Percepção do Tato/genética , Canais de Potencial de Receptor Transitório/genética , Animais , Hemípteros/genética , Hemípteros/crescimento & desenvolvimento , Proteínas de Insetos/metabolismo , Mecanotransdução Celular/genética , Ninfa/genética , Ninfa/fisiologia , Óvulo/fisiologia , RNA de Cadeia Dupla/metabolismo , Canais de Potencial de Receptor Transitório/metabolismo
14.
Pest Manag Sci ; 75(2): 492-505, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30070049

RESUMO

BACKGROUND: The European woodwasp, Sirex noctilio, is a global invasive pest, attacking a wide variety of pine species by inoculating spores of a symbiotic fungus (Amylostereum areolatum) at oviposition. The woodwasp larvae depend on the growth of the symbiotic fungus to feed. The relationship among host endophytic fungi, symbiotic fungus and woodwasp remain elusive. Here, the effects of endophytes in Mongolian pine on the growth of Amylostereum areolatum and the selection behavior of female woodwasp were investigated by quantifying the mycelium growth rates and olfactometry assays. RESULTS: The endophytic plant fungi, Trichoderma harzianum, Phlebiopsis gigantea, T. viride and T. atroviride, completely killed the mycelia of Amylostereum areolatum. Mycelium fermentation broth of Chaetomium globosum inhibited the growth of the symbiont. Moreover, we observed that volatiles of Ophiostoma minus and Aspergillus niger (acetophenone, acetylacetone, hexadecane, phenylethyl alcohol, and isopropyl myristate) had repellent effects on adult female woodwasp. While volatiles of Amylostereum areolatum ((-)-globulol, 2-hexene, cycloprop[e]indene-1a,2(1H)-dicarboxaldehyde, terpene and cyclopentanone) had a significant attractiveness to adult female woodwasp. CONCLUSIONS: Some species of the host endophytic fungi had a significant negative effect on the growth and development of woodwasps, which could be useful in the monitoring and effective management of woodwasps. © 2018 Society of Chemical Industry.


Assuntos
Basidiomycota/fisiologia , Endófitos/fisiologia , Himenópteros/fisiologia , Oviposição , Pinus/microbiologia , Pinus/fisiologia , Animais , Basidiomycota/crescimento & desenvolvimento , Endófitos/crescimento & desenvolvimento , Feminino , Simbiose
15.
Zhongguo Gu Shang ; 31(5): 446-451, 2018 May 25.
Artigo em Chinês | MEDLINE | ID: mdl-29890805

RESUMO

OBJECTIVE: To investigate the clinical effect of drilling columnar autogenous iliac bone graft and analyze the effect of bone grafting on postoperative complications in donor site. METHODS: From March 2014 to October 2016, 68 patients with autogenous iliac bone graft were retrospectively analyzed, and divided into drilling group and osteotomy group, 34 patients in each group. In drilling group, there were 24 males and 10 females with an average age of (40.06±5.60) years old ranging from 23 to 53 years old;in osteotomy group, there were 26 males and 18 females with an average age of (39.32±6.44) ranging from 22 to 56 years old. The operative time of bone extraction, blood loss in donor area, healing time of donor site and postoperative donor site complications were observed and compared between the two groups. VAS score was used to evaluate the pain of donor site in different periods after operation. RESULTS: All patients were followed up for 12 to 24 months, with an average of 16.9 months in drilling group and 17.1 months in osteotomy groups. The bone healing structure was displayed in the recipient area in two groups, the effect of autogenous iliac bone grafting was good. There was no significant difference in operative time between two groups (P>0.05). There was significant difference between two groups in the amount of donor site bleeding and the time of donor site wound healing(P<0.05). Postoperative complications(iliac depression and numbness) were significantly different between two groups (P<0.05). There was no significant difference in VAS score between two groups at 2 weeks after operation(P>0.05). VAS scores of drilling group at 6 months and 1 year after operation were lower than those of osteotomy group (1.85±0.61 vs 2.97±0.67, P=0.000; 1.15±0.56 vs 2.41±0.61, P=0.000). CONCLUSIONS: When bone graft is no need to have large pieces of special shape or more cortical bone iliac, it is simple to operate and less complications postoperative by drilling type columnar autogenous iliac bone graft. What's more, it has the obvious advantages of promote healing, improve patient quality of life compared with traditional osteotomy.


Assuntos
Transplante Ósseo , Qualidade de Vida , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Dor Pós-Operatória , Complicações Pós-Operatórias , Estudos Retrospectivos , Adulto Jovem
16.
BMC Complement Altern Med ; 17(1): 365, 2017 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-28709426

RESUMO

BACKGROUND: Retinal ischemia-related eye diseases result in visual dysfunction. This study investigates the protective effects and mechanisms of Xue-Fu-Zhu-Yu decoction (XFZYD) with respect to retinal ischemia. METHODS: Retinal ischemia (I) was induced in Wistar rats by a high intraocular pressure (HIOP) of 120 mmHg for 1 h, which was followed by reperfusion of the ischemic eye; the fellow untreated eye acted as a control. Electroretinogram (ERG), biochemistry and histopathology investigations were performed. RESULTS: Significant ischemic changes occurred after ischemia including decreased ERG b-wave ratios, less numerous retinal ganglion cells (RGCs), reduced inner retinal thickness, fewer choline acetyltransferase (ChAT) labeled amacrine cell bodies, increased glial fibrillary acidic protein (GFAP) immunoreactivity and increased vimentin Müller immunolabeling. These were accompanied by significant increases in the mRNA/protein concentrations of vascular endothelium growth factor, hypoxia-inducible factor-1α, pyruvate kinase M2 and retinoblastoma-binding protein 2. The ischemic changes were concentration-dependently and significantly altered when XFZYD was given for seven consecutive days before or after retina ischemia, compared to vehicle. These alterations included enhanced ERG b-wave amplitudes, more numerous RGCs, enhanced inner retinal thickness, a greater number of ChAT immunolabeled amacrine cell bodies and decreased GFAP/vimentin immunoreactivity. Furthermore, decreased mRNA levels of VEGF, HIF-1α, PKM2, and RBP2 were also found. Reduced protein concentrations of VEGF, HIF-1α, PKM2, and RBP2 were also demonstrated. Furthermore, there was an inhibition of the ischemia-associated increased ratios (target protein/ß-actin) in the protein levels of VEGF, HIF-1α, PKM2, and RBP2, which were induced by Shikonin, JIB-04 or Avastin. CONCLUSION: XFZYD would seem to protect against well-known retinal ischemic changes via a synergistic inhibition of RBP2 and PKM2, as well as down-regulation of HIF-1α and a reduction in VEGF secretion.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Isquemia/tratamento farmacológico , Fitoterapia , Retina/efeitos dos fármacos , Doenças Retinianas/metabolismo , Animais , Regulação para Baixo , Medicamentos de Ervas Chinesas/uso terapêutico , Eletrorretinografia , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Isquemia/metabolismo , Masculino , Piruvato Quinase/metabolismo , Ratos Wistar , Retina/metabolismo , Retina/patologia , Doenças Retinianas/tratamento farmacológico , Proteína 2 de Ligação ao Retinoblastoma/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo
17.
Am J Emerg Med ; 35(12): 1892-1894, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28648673

RESUMO

BACKGROUND: Chest compression is a standard recommendation during cardiopulmonary resuscitation (CPR). However, chest compression cannot be effectively applied under certain situations, such as chest wall deformity, rib fracture, or hemopneumothorax. An alternative method, abdominal compression, was reported to achieve better resuscitation outcomes in these patients. MATERIALS AND METHODS: A prospective study was performed in adult patients with cardiac arrest and anticipated ineffective chest compression (thoracic trauma, chest deformity, rib fracture, and hemopneumothorax). Active abdominal lifting and compression cardiopulmonary resuscitation was used. Primary outcome was success rate of restoration of spontaneous circulation (ROSC). Secondary outcomes included heart rate (HR), mean arterial pressure (MAP), pulse oximetry saturation (SpO2), arterial blood pH value, arterial oxygen pressure (PaO2), and arterial carbon dioxide tension (PaCO2), which were measured during the periods of pre-CPR, CPR, and 30min post-ROSC. RESULTS: A total of 35 patients were enrolled into the study. Five of them had ROSC (14.3%), which was statistically significantly higher than that (0%) reported in the 2015 Advanced Cardiovascular Life Support manual. HR, MAP, and SpO2 during CPR were also statistically significantly higher during CPR when compared to the period of pre-CPR period (HR 58 versus 0 beats/min, P<0.01; MAP 25 versus 0mm Hg, P<0.01; SpO2 0.68 versus 0.48%, P<0.01). In post-ROSC period, HR was statistically significantly higher than that during pre-CPR period (121 versus 0 best/min, P<0.01). CONCLUSIONS: Active abdominal lifting and compression cardiopulmonary resuscitation could reach better resuscitation outcomes in certain cardiac arrest patients.


Assuntos
Abdome , Reanimação Cardiopulmonar , Parada Cardíaca/terapia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Pressão Arterial , Reanimação Cardiopulmonar/métodos , China , Feminino , Parada Cardíaca/fisiopatologia , Humanos , Masculino , Pessoa de Meia-Idade , Avaliação de Resultados em Cuidados de Saúde , Estudos Prospectivos , População Urbana , Adulto Jovem
18.
Pest Manag Sci ; 73(3): 590-597, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27302648

RESUMO

BACKGROUND: Glutamate-gated chloride channels (GluCl) mediate fast inhibitory neurotransmission in invertebrate nervous systems. Although only one GluCl gene was presented in insects, it showed diverse alternative splicing that was speculated could affect channel function and pharmacology. RESULTS: In this study, we isolated GluCl cDNAs from adults of the small brown planthopper (SBPH) Laodelphax striatellus and showed that six L. striatellus GluCl variants (LsGluCl-AS, LsGluCl-BS, LsGluCl-CS, LsGluCl-AL, LsGluCl-BL, LsGluCl-CL) were present in the SBPH. The expression patterns of six variants differed among developmental stages (egg, first- to fifth-instar nymphs, male and female adults) and among the body parts (head, thorax, abdomen, leg) of the female adult SBPH. All the transcripts were abundant in the head of the adult. When expressed in African clawed frog, Xenopus laevis, oocytes, the two functional variants (LsGluCl-AS, LsGluCl-AL) had similar EC50 and IC50 values for L-glutamate and channel blockers picrotoxinin and fipronil. CONCLUSION: This study represents a comprehensive molecular, expression and pharmacological characterisation of GluCl in the SBPH. These findings should be useful in providing more opportunities to discover novel insect control chemicals. © 2016 Society of Chemical Industry.


Assuntos
Canais de Cloreto/genética , Antagonistas de Receptores de GABA-A/farmacologia , Hemípteros/efeitos dos fármacos , Hemípteros/genética , Proteínas de Insetos/genética , Inseticidas/farmacologia , Processamento Alternativo , Sequência de Aminoácidos , Animais , Sequência de Bases , Canais de Cloreto/química , Canais de Cloreto/metabolismo , Clonagem Molecular , DNA Complementar/genética , DNA Complementar/metabolismo , Feminino , Ácido Glutâmico/metabolismo , Hemípteros/crescimento & desenvolvimento , Hemípteros/metabolismo , Proteínas de Insetos/química , Proteínas de Insetos/metabolismo , Dose Letal Mediana , Masculino , Ninfa/efeitos dos fármacos , Ninfa/genética , Ninfa/crescimento & desenvolvimento , Ninfa/metabolismo , Picrotoxina/análogos & derivados , Picrotoxina/farmacologia , Pirazóis/farmacologia , Alinhamento de Sequência
19.
Pest Manag Sci ; 73(8): 1618-1624, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27943653

RESUMO

BACKGROUND: Laodelphax striatellus (Fallén) is an important pest of crops in East Asia. Over the past decade, phenylpyrazole insecticides, which target the insect GABA receptor, have increasingly been used as alternatives against rice planthoppers. RESULTS: Cross-resistance to ethiprole and butene-fipronil was detected in a laboratory-selected fipronil-resistant strain of L. striatellus (LsFR). Compared with a fipronil-susceptible strain (LsFS), LsFR had obtained a high-level resistance to fipronil (112.1-fold) and moderate resistance to ethiprole (24.5-fold) and butene-fipronil (14.7-fold). For the resistance of field populations, LC50 values of ethiprole were remarkably higher than the other two analogues in Gaochun and Yancheng populations in 2016. Significant correlations were demonstrated between the LC50 values of three phenylpyrazole insecticides (R = 0.944-0.998, P = 0.007-0.016). Additionally, an AS-PCR assay was developed to detect the A2'N mutant GABA receptor in L. striatellus strains or populations. It was noteworthy that mutation frequencies of 19.2 and 3.6% appeared in Lujiang and Gaochun populations in 2016, respectively. Furthermore, there was an extremely significant difference in genomic expression of Lsrdl between the LsFS and LsFR individuals (1.85-fold, F = 26.8, P = 0.0008). CONCLUSIONS: This study could help us better understand the cross-resistance mechanisms in L. striatellus, and be beneficial for proposing effective pest management strategies of phenylpyrazole resistance. © 2016 Society of Chemical Industry.


Assuntos
Hemípteros/genética , Resistência a Inseticidas/genética , Inseticidas , Mutação , Pirazóis , Receptores de GABA-A/genética , Animais , Duplicação Gênica , Genes de Insetos/genética
20.
Pestic Biochem Physiol ; 134: 8-13, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27914544

RESUMO

Three rice planthoppers, brown planthopper, Nilaparvata lugens, white-backed planthopper, Sogatella furcifera and small brown planthopper, Laodelphax striatellus, are important pests of cultivated rice in tropical and temperate Asia. They have caused severe economic loss and developed resistance to insecticides from most chemical classes. Dinotefuran is the third neonicotinoid which possesses a broad spectrum and systemic insecticidal activity. We determined the susceptibility of dinotefuran to field populations from major rice production areas in China from 2013 to 2015. All the populations of S. furcifera and L. striatellus were kept susceptible to dinotefuran (0.7 to 1.4-fold of S. furcifera and 1.1-to 3.4-fold of L. striatellus) However, most strains of N. lugens (except FQ15) collected in 2015 had developed moderate resistance to dinotefuran, with resistance ratios (RR) ranging from 23.1 to 100.0 folds. Cross-resistance studies showed that chlorpyrifos-resistant and buprofezin-resistant Sogatella furcifera, chlorpyrifos-resistant and fipronil-resistant L. striatellus, imidacloprid-resistant and buprofezin-resistant Nilaparvata lugens exhibited negligible or no cross-resistance to dinotefuran. Synergism tests showed that piperonyl butoxide (PBO) produced a high synergism of dinotefuran effects in the DY15 and JS15 populations (2.14 and 2.52-fold, respectively). The obvious increase in resistance to dinotefuran in N. lugens indicates that insecticide resistance management strategies are urgently needed to prevent or delay further increase of insecticide resistance in N. lugens.


Assuntos
Guanidinas/toxicidade , Hemípteros/efeitos dos fármacos , Resistência a Inseticidas , Inseticidas/toxicidade , Nitrocompostos/toxicidade , Animais , China , Imidazóis/toxicidade , Neonicotinoides , Oryza , Sinergistas de Praguicidas/toxicidade , Butóxido de Piperonila/toxicidade , Pirazóis/toxicidade , Tiadiazinas/toxicidade
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