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1.
Transl Psychiatry ; 12(1): 76, 2022 02 23.
Artigo em Inglês | MEDLINE | ID: mdl-35197458

RESUMO

Attention-deficit/hyperactivity disorder (ADHD) is a common childhood mental disorder with undetermined pathophysiological mechanisms. The gut microbiota and immunological dysfunction may influence brain functions and social behaviours. In the current study, we aimed to explore the correlation of gut microbiome imbalance and inflammation in the pathophysiology of ADHD. Forty-one children with ADHD and thirty-nine healthy-control (HC) individuals were recruited. Faecal samples from all participants were collected and submitted for 16 S rRNA V3-V4 amplicon microbiome sequencing analysis. The plasma levels of 10 cytokines, including TNF-α, IL-6, IL-1ß, IL-2, IL-10, IL-13, IL-17A, IFN-α2, IFN-γ, and MCP-1, were determined using a custom-made sandwich enzyme-linked immunosorbent assay (ELISA) developed by Luminex Flowmetrix. There was no significant difference between the ADHD and HC groups in species diversity in the faeces, as determined with α-diversity and ß-diversity analysis. In the ADHD group, three differentially abundant taxonomic clades at the genus level were observed, namely Agathobacter, Anaerostipes, and Lachnospiraceae. Top differentially abundant bacteria and representative biological pathways were identified in children with ADHD using linear discriminant analysis (LDA) effect size (LEfSe), and the phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis, respectively. The plasma levels of TNF-α were significantly lower in children with ADHD than in HCs. Within the ADHD group, the levels of TNF-α were negatively correlated with ADHD symptoms and diversity of the gut microbiome. Our study provides new insights into the association between gut microbiome dysbiosis and immune dysregulation, which may contribute to the pathophysiology of ADHD.


Assuntos
Transtorno do Deficit de Atenção com Hiperatividade , Microbioma Gastrointestinal , Criança , Citocinas/genética , Disbiose , Microbioma Gastrointestinal/genética , Humanos , Filogenia
2.
Rev. bras. med. esporte ; 28(6): 690-693, Nov.-Dec. 2022. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1376721

RESUMO

ABSTRACT Introduction Sports injury is a common injury among professional tennis players compared to other sports. Updated rehabilitation methods can accelerate players' recovery and ensure a return to sporting activity. However, contemporary rehabilitation monitoring practices want updated reporting. Objective Monitoring tennis players' function and recovery methods during rehabilitation training after injury. Methods The tennis player underwent arthroscopic capsulotomy of the left ankle joint, free body removal, and synovectomy. The athlete's body composition, blood routine, biochemistry, nutritional indices, and physiological indicators were monitored. Data were collected before, during, and at the end of the four months (6-10 months) of rehabilitation after the operation. Results 1 month after the operation, weight and lean mass decreased significantly; body fat percentage increased; static heart rate increased significantly (P<0.05). Three months after surgery, the athlete's lean mass increased significantly to 43.7 kg; body fat percentage decreased to 24.5% (P<0.05); hemoglobin, serum iron, total protein, and albumin improved; in rehabilitation during October, blood routine and biochemical blood indices were regular; Resting, maintained-load and recovery heart rate decreased significantly (P<0.05). Conclusion Tennis players should begin function monitoring, rehabilitation training, and nutritional recovery as soon as possible after ankle joint surgery. Evidence Level II; Therapeutic Studies - Investigating the result.


RESUMO Introdução A lesão esportiva é uma lesão comum entre os tenistas profissionais, em comparação com outros esportes. Métodos atualizados de reabilitação podem acelerar a recuperação dos jogadores e garantir um retorno à atividade esportiva. Porém o monitoramento das práticas de reabilitação contemporâneas carece de relatórios atualizados. Objetivo Explorar o monitoramento da função e métodos de recuperação dos jogadores de tênis durante o treinamento de reabilitação após uma lesão. Métodos O tenista foi submetido a capsulotomia artroscópica da articulação do tornozelo esquerdo, remoção de corpo livre e sinovectomia. Foram monitorados a composição corporal do atleta, rotina sanguínea, bioquímica sanguínea, indicadores nutricionais e indicadores fisiológicos. Esses dados foram coletados antes, durante e ao final dos 4 meses (6-10 meses) de reabilitação após a operação. Resultados 1 mês após a operação, o peso e a massa magra diminuíram significativamente, aumentando a porcentagem de gordura corporal; o ritmo cardíaco estático aumentou significativamente (P<0,05). 3 meses após a cirurgia, a massa magra do atleta aumentou significativamente para 43,7 kg, a porcentagem de gordura corporal caiu para 24,5% (P<0,05); a hemoglobina, o ferro sérico, a proteína total e a albumina melhoraram. Na reabilitação durante o mês de outubro, a rotina sanguínea e os índices bioquímicos do sangue foram normais; a frequência cardíaca em repouso, com carga mantida e de recuperação diminuiu significativamente (P<0,05). Conclusão Os tenistas devem começar o monitoramento das funções, treinamento de reabilitação e recuperação nutricional o mais rápido possível após uma cirurgia na articulação do tornozelo. Nível de evidência II; Estudos Terapêuticos - Investigação de Resultados.


RESUMEN Introducción La lesión deportiva es una lesión común entre los tenistas profesionales en comparación con otros deportes. Los métodos de rehabilitación actualizados pueden acelerar la recuperación de los jugadores y garantizar la vuelta a la actividad deportiva. Sin embargo, el seguimiento de las prácticas contemporáneas de rehabilitación carece de informes actualizados. Objetivo Explorar el seguimiento de la función y los métodos de recuperación de los tenistas durante el entrenamiento de rehabilitación después de una lesión. Métodos El tenista fue sometido a una capsulotomía artroscópica de la articulación del tobillo izquierdo, a la extracción del cuerpo libre y a la sinovectomía. Se controló la composición corporal del atleta, la rutina sanguínea, la bioquímica sanguínea, los indicadores nutricionales y los indicadores fisiológicos. Estos datos se recogieron antes, durante y al final de los 4 meses (6-10 meses) de rehabilitación tras la operación. Resultados 1 mes después de la operación, el peso y la masa magra disminuyeron significativamente, aumentando el porcentaje de grasa corporal; la frecuencia cardíaca estática aumentó significativamente (P<0,05). 3 meses después de la operación, la masa magra de la atleta aumentó significativamente hasta 43,7 kg, el porcentaje de grasa corporal disminuyó hasta el 24,5% (P<0,05); la hemoglobina, el hierro sérico, la proteína total y la albúmina mejoraron. En el periodo de entrenamiento de rehabilitación en el mes de octubre, la rutina sanguínea y los índices bioquímicos sanguíneos fueron normales; la frecuencia cardíaca en reposo, con carga mantenida y en recuperación disminuyó significativamente (P<0,05). Conclusión Los jugadores de tenis deben comenzar el control de la función, el entrenamiento de rehabilitación y la recuperación nutricional lo antes posible después de la cirugía de la articulación del tobillo. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

3.
J Am Chem Soc ; 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35914188

RESUMO

Although the mass production of synthetic plastics has transformed human lives, it has resulted in waste accumulation on the earth. Here, we report a low-temperature conversion of polyethylene into olefins. By mixing the polyethylene feed with rationally designed ZSM-5 zeolite nanosheets at 280 °C in flowing hydrogen as a carrier gas, light hydrocarbons (C1-C7) were produced with a yield of up to 74.6%, where 83.9% of these products were C3-C6 olefins with almost undetectable coke formation. The reaction proceeds in multiple steps, including polyethylene melting, flowing to access the zeolite surface, cracking on the zeolite surface, formation of intermediates to diffuse into the zeolite micropores, and cracking into small molecules in the zeolite micropores. The ZSM-5 zeolite nanosheets kinetically matched the cascade cracking steps on the zeolite external surface and within micropores by boosting the intermediate diffusion. This feature efficiently suppressed the intermediate accumulation on the zeolite surface to minimize coke formation. In addition, we found that hydrogen participation in the cracking process could hinder the formation of polycyclic species within zeolite micropores, which also contributes to the rapid molecule diffusion. The coking-resistant polyethylene upcycling process at a low temperature not only overturns the general viewpoint for facile coke formation in the catalytic cracking over the zeolites but also demonstrates how the polyethylene-based plastics can be upcycled to valuable chemicals. In addition to the model polyethylene, the reaction system worked efficiently for the depolymerization of multiple practically used polyethylene-rich plastics, enabling an industrially and economically viable path for dealing with plastic wastes.

4.
J Food Biochem ; : e14351, 2022 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-35929638

RESUMO

Heart failure (HF) is a serious disease with high mortality. Oxidative stress plays a vital role in its occurrence and development. Licorice is commonly used to treat HF in traditional Chinese medicine. Liquiritin, the main ingredient of licorice, has antioxidant and anti-inflammatory properties, but the mechanism against oxidative stress in cardiomyocytes has not been reported. Establishment of oxidative damage model in H9c2 cells by hydrogen peroxide (H2 O2 ). Liquiritin (5, 10, 20 µmol/L) could significantly prevent the loss of cell viability and decrease the apoptosis rate. It can reduce the levels of reactive oxygen species (ROS), malonedialdehyde (MDA), lactate dehydrogenase (LDH), tumor necrosis factor-alpha (TNF-α), interleukin-1ß (IL-1ß), interleukin-6 (IL-6), and increase the activity of ATP, superoxidedismutase (SOD), glutathione peroxide (GSH-px), glutathione reductase (GR) and catalase (CAT) to alleviate oxidative stress and inflammation in a dose-dependent manner. Liquiritin was found to be related to AMP-Activated Protein Kinase (AMPK) pathway by molecular docking. Western blotting (WB) and quantitative reverse transcription PCR (RT-qPCR) confirmed that liquiritin could promote AMPKα phosphorylation and sirtuin 1 (SIRT1) protein expression, and inhibit phosphorylation of nuclear factor kappa B p65 (NF-κB p65). Compound C, EX 527, and PDTC can reverse the effects of liquiritin, indicating that its antioxidant effect is achieved by regulating AMPK/SIRT1/NF-κB signaling pathway. PRACTICAL APPLICATIONS: Heart failure is one of the most common cardiovascular diseases, and its treatment remains a worldwide problem. Licorice is a food and dietary supplement that has been used widely in traditional Chinese medicine (TCM). Liquiritin is one of the main active components of licorice, which has antioxidant and anti-inflammatory pharmacological effects. This study revealed the mechanism of licorice against oxidative damage of H9c2 cardiomyocytes, and provided a scientific basis for liquiritin as an antioxidant in the treatment of heart failure.

5.
Arch Biochem Biophys ; : 109371, 2022 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-35921901

RESUMO

In this study, we investigated whether modification of the carboxyl group with semicarbazide-enabled myoglobin (Mb) exhibits membrane-perturbing activity in physiological solutions. Mass spectrometry analysis showed that semicarbazide molecules were coupled to 19 of the 22 carboxyl groups in semicarbazide-modified Mb (SEM-Mb). Measurements of the absorption and circular dichroism spectra indicated that SEM-Mb lost its heme group and reduced the content of the α-helix structure in Mb. The microenvironment surrounding Trp residues in Mb changes after blocking negatively charged residues, as shown by fluorescence quenching studies. The results of the trifluoroethanol-induced structural transition indicated that SEM-Mb had higher structural flexibility than that of Mb. SEM-Mb, but not Mb, induced the permeability of bilayer membranes. Both proteins showed similar lipid-binding affinities. The conformation of SEM-Mb and Mb changed upon binding to lipid vesicles or a membrane-mimicking environment composed of SDS micelles, suggesting that membrane interaction modes differ. Unlike lipid-bound Mb, Trp residues in lipid-bound SEM-Mb are located at the protein-lipid interface. Altogether, our data indicate that modifying negatively charged groups relieves the structural constraints in Mb, consequently switching Mb structure to an active conformation that exhibits membrane-permeabilizing activity.

6.
Proteins ; 2022 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-35924777

RESUMO

ATP-binding cassette (ABC) superfamily is one of the largest groups of primary active transporters that could be found in all kingdoms of life from bacteria to humans. In human, ABC transporters can selectively transport a wide spectrum of substrates across membranes, thus playing a pivotal role in multiple physiological processes. In addition, due to the ability of exporting clinic therapeuticals, some ABC transporters were originally termed multidrug resistance proteins. Increasing investigations of human ABC transporters in recent years have provided abundant information for elucidating their structural features, based on the structures at distinct states in a transport cycle. This review focuses on the recent progresses in human ABC structural analyses, substrate binding specificities and translocation mechanisms. We dedicate to summarize the common features of human ABC transporters in different subfamilies, and to discuss the possibility to apply the fast-developing techniques, such as cryogenic electron microscopy, artificial intelligence-assisted structure prediction, for future studies. This article is protected by copyright. All rights reserved.

7.
Artigo em Inglês | MEDLINE | ID: mdl-35915306

RESUMO

The global spread of COVID-19, international trade protectionism, geopolitical conflicts, and climate change presents challenges and risks to sustainable supply chains (SSCs). In recent years, scholarly interest in sustainable supply chain risk management (SSCRM) has continued to rise. A helpful literature review is necessary to enable supply chain practitioners to apply empirical findings from academic research or conceptual frameworks to their operations to maintain the stability and competitiveness of sustainable supply chains. The knowledge map of SSCRM is explored in this study using both quantitative and qualitative analysis. A total of 793 articles were retrieved to reveal the knowledge map of SSCRM. Scientometric and context analysis are combined in quantitative analysis to identify the intellectual structure of risk management research related to SSC. Then, a critical review is conducted in qualitative analysis to summarize and analyze the motivations, strategies, approaches, and tools of SSCRM. Combining the quantitative and qualitative analysis results, a conceptual model is constructed for SSCRM from three aspects: (1) risk identification, (2) risk assessment, and (3) risk mitigating and responding. Finally, future research directions are suggested based on the conceptual model for guiding the theories and practice of SSCRM. This study can work as a roadmap for providing appropriate risk management policies and toolkits to SSC, which could advance theoretical thinking on how to mitigate SSC risks.

8.
Cell Death Dis ; 13(8): 671, 2022 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-35918330

RESUMO

Sustained expression of programmed cell death receptor-1 (PD-1) is correlated with the exhaustion of T cells, and blockade of the PD-1 pathway is an effective immunotherapeutic strategy for treating various cancers. However, response rates are limited, and many patients do not achieve durable responses. Thus, it is important to seek additional strategies that can improve anticancer immunity. Here, we report that the bromodomain and extraterminal domain (BET) inhibitor JQ1 inhibits PD-1 expression in Jurkat T cells, primary T cells, and T-cell exhaustion models. Furthermore, JQ1 dramatically impaired the expression of PD-1 and T-cell immunoglobulin mucin-domain-containing-3 (Tim-3) and promoted the secretion of cytokines in T cells from patients with acute myeloid leukemia (AML). In line with that, BET inhibitor-treated CD19-CAR T and CD123-CAR T cells have enhanced anti-leukemia potency and resistant to exhaustion. Mechanistically, BRD4 binds to the NFAT2 and PDCD1 (encoding PD-1) promoters, and NFAT2 binds to the PDCD1 and HAVCR2 (encoding Tim-3) promoters. JQ1-treated T cells showed downregulated NFAT2, PD-1, and Tim-3 expression. In addition, BET inhibitor suppressed programmed death-ligand 1 (PD-L1) expression and cell growth in AML cell lines and in primary AML cells. We also demonstrated that JQ1 treatment led to inhibition of leukemia progression, reduced T-cell PD-1/Tim-3 expression, and prolonged survival in MLL-AF9 AML mouse model and Nalm6 (B-cell acute lymphoblastic leukemia cell)-bearing mouse leukemia model. Taken together, BET inhibition improved anti-leukemia immunity by regulating PD-1/PD-L1 expression, and also directly suppressed AML cells, which provides novel insights on the multiple effects of BET inhibition for cancer therapy.


Assuntos
Antígeno B7-H1 , Leucemia Mieloide Aguda , Animais , Linhagem Celular Tumoral , Receptor Celular 2 do Vírus da Hepatite A , Leucemia Mieloide Aguda/tratamento farmacológico , Camundongos , Proteínas Nucleares/uso terapêutico , Receptor de Morte Celular Programada 1 , Fatores de Transcrição/uso terapêutico
9.
Front Neurosci ; 16: 918767, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35937874

RESUMO

Offline perceptions are self-generated sensations that do not involve physical stimulus. These perceptions can be induced by external hallucinated objects or internal imagined objects. However, how the brain dissociates these visual sensations remains unclear. We aimed to map the brain areas involved in internal and external visual sensations induced by intracranial electrical stimulation and further investigate their neural differences. In this study, we collected subjective reports of internal and external visual sensations elicited by electrical stimulation in 40 drug-refractory epilepsy during presurgical evaluation. The response rate was calculated and compared to quantify the dissociated distribution of visual responses. We found that internal and external visual sensations could be elicited when different brain areas were stimulated, although there were more overlapping brain areas. Specifically, stimulation of the hippocampus and inferior temporal cortex primarily induces internal visual sensations. In contrast, stimulation of the occipital visual cortex mainly triggers external visual sensations. Furthermore, compared to that of the dorsal visual areas, the ventral visual areas show more overlap between the two visual sensations. Our findings show that internal and external visual sensations may rely on distinct neural representations of the visual pathway. This study indicated that implantation of electrodes in ventral visual areas should be considered during the evaluation of visual sensation aura epileptic seizures.

10.
JAMA Netw Open ; 5(8): e2225438, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35925605

RESUMO

Importance: Risk of relapse may be increased in the postpartum period of neuromyelitis optica spectrum disorder (NMOSD). Information regarding factors associated with pregnancy-related attacks is still lacking. Objectives: To identify factors associated with pregnancy-related NMOSD attacks, investigate the integrated annualized relapse rate (ARR) and Expanded Disability Status Scale (EDSS) score in each phase of pregnancy, and summarize pregnancy outcomes and complications in patients with NMOSD. Data Sources: An electronic search was performed in the MEDLINE, PubMed in-process and non-MEDLINE, EMBASE, Web of Science, and Cochrane databases using the OvidSP search platform, updated through December 30, 2021. Study Selection: All published and unpublished studies in English were considered, covering all patients with NMOSD with an informative pregnancy. Data Extraction and Synthesis: Two independent reviewers extracted the published data with a standardized procedure following MOOSE and PRISMA guidelines. The end points were calculated with the DerSimonian and Laird inverse variance (for random effects) method. Main Outcomes and Measures: The primary outcome was the rate of pregnancies with pregnancy-related NMOSD attacks, measured by risk ratios (RRs). The mean differences (MDs) in ARR and EDSS scores between each phase of pregnancy, pregnancy outcomes, and complications were defined as the secondary outcomes. Results: A total of 15 studies were analyzed, including 443 patients with NMOSD with 639 informative pregnancies. Patients receiving immunosuppressive treatment during pregnancy (RR, 0.43; 95% CI, 0.32-0.57; P < .001) and with older age at conception (RR, 0.67; 95% CI, 0.47-0.95; P = .02) had lower rates of pregnancy with pregnancy-related attacks. The increase in the ARR was highest in the first trimester after delivery compared with before pregnancy (MD, 1.28; 95% CI, 0.94-1.62; P < .001). The EDSS scores increased significantly both during pregnancy (MD, 0.44; 95% CI, 0.20-0.69; P < .001) and in the postpartum period (MD, 0.88; 95% CI, 0.51-1.26; P < .001) compared with before pregnancy. Conclusions and Relevance: This systematic review and meta-analysis found that receiving immunosuppressive treatment during pregnancy and older age at conception were associated with reduced risk of pregnancy-related NMOSD attacks, which mostly occurred in the first trimester of the postpartum period, although more high-quality prospective studies are needed.


Assuntos
Neuromielite Óptica , Feminino , Humanos , Imunossupressores/efeitos adversos , Recidiva Local de Neoplasia/induzido quimicamente , Neuromielite Óptica/induzido quimicamente , Neuromielite Óptica/epidemiologia , Neuromielite Óptica/terapia , Período Pós-Parto , Gravidez , Resultado da Gravidez/epidemiologia
11.
Ultrasonics ; 126: 106806, 2022 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-35914377

RESUMO

As the thermonuclear fuel container, the inertial confinement fusion (ICF) microspheres must be detected before the ICF experiment to maximize the profits of fusion reactions. However, in the current detection method, the ICF microsphere is in direct contact with the measurement platform, resulting in the ICF microsphere surface being easily damaged during the detection process. In this paper, an ultrasonic manipulation method is proposed, realizing non-destructive, high-precision, and high-efficient manipulation of the ICF microsphere by switching the two acoustic fields produced in the liquid. When detecting the ICF microsphere, the first acoustic field (1st AF) accurately traps the microsphere in the acoustic field center to achieve its precise positioning. And when the ICF microsphere is failed to pass the detection, it is transferred out of the microscope measurement area by switching to the second acoustic field (2nd AF). Two solid vibration modes, their corresponding acoustic fields, and the two acoustic streaming fields are first computed by the finite element method. Then, the manipulation experiments indicated that the ICF microspheres can be first driven to the center of the 1st AF and then be positioned here with a minimum positional fluctuation of 1.1 µm. By changing to the 2nd AF, the positioned microsphere can be transferred nearly 11 mm to the nearest antinode from the acoustic field center. Finally, based on the proposed ultrasonic manipulation method, the detection experiments of the ICF microsphere were carried out, illustrating that the positioning of the 1st AF meets the requirements of the morphology detection and the radius measurement of the ICF microsphere. The proposed method holds the advantages of non-destructive, high-precision, simple control scheme and meets the practical application needs of the microsphere fixed-point detection, presenting the potential promise for the field of microsphere detection.

12.
Int J Med Sci ; 19(8): 1265-1274, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35928722

RESUMO

Objective: To investigate the efficiency and potential mechanisms of exosomes from dendritic cells (DCs) transfected with Forkhead box protein P3 (FOXP3) in the development of experimental autoimmune encephalomyelitis (EAE). Method: Mouse bone marrow-derived immature DCs were loaded with adenovirus carrying FOXP3 gene, and exosomes were generated. Then the exosomes with FOXP3 (FOXP3-EXOs) were co-cultured with CD4+T cell in vitro to evaluate their potential on CD4+T cell proliferation and differentiation, and injected into EAE mice to assess their effects on the development of EAE. Result: FOXP3-EXOs were effective to inhibit the CD4+T cell proliferation and the production of Interferon gamma (IFN-γ), interleukin (IL)-6, and IL-17, while they promoted the production of IL-10 in vitro. Moreover, FOXP3-EXOs treatment significantly decreased the neurological scores, reduced the infiltration of inflammatory cells into the spinal cord, and decreased demyelination in comparison to saline and Con-EXOs treated EAE mice. Moreover, the FOXP3-EXOs treatment resulted in obvious increases in the levels of regulatory T (Treg) cells and IL-10, whereas levels of T helper 1 (Th1) cells, Th17 cells, IFN-γ, IL-6, and IL-17 decreased significantly in the splenocyte culture of EAE mice. Conclusion: The present study preliminarily investigated the effects and potential mechanisms of FOXP3-EXOs in EAE and revealed that the FOXP3-EXOs could inhibit the production of Th1 and Th17 cells and promote the production of Treg cells as well as ameliorate the development of EAE. The neuroprotective effects of FOXP3-EXOs on EAE are likely due to the regulation of Th/Treg balance.


Assuntos
Encefalomielite Autoimune Experimental , Exossomos , Animais , Células Dendríticas , Encefalomielite Autoimune Experimental/genética , Encefalomielite Autoimune Experimental/terapia , Exossomos/genética , Exossomos/metabolismo , Fatores de Transcrição Forkhead/genética , Fatores de Transcrição Forkhead/metabolismo , Interferon gama/metabolismo , Interleucina-10/genética , Interleucina-10/metabolismo , Interleucina-17/metabolismo , Interleucina-6/genética , Interleucina-6/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Linfócitos T Reguladores , Células Th17
14.
Diabetes Metab Syndr Obes ; 15: 2235-2247, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35936054

RESUMO

Purpose: To evaluate the predictive effect of the initial weight loss on the long-term weight loss in Chinese patients with a body mass index (BMI) ≥ 32.5 kg/m2 who underwent LSG. Patients and Methods: The follow-up was completed via phone or WeChat for outpatients and at the hospital for inpatients. We evaluated the BMI, percentage of excess weight loss (%EWL), and type 2 diabetes mellitus, hypertension, and hyperlipidemia statuses. Linear and logistic regression analyses were performed on the relationship between the initial and long-term weight loss. The optimal cut-off value was determined by receiver operating characteristic (ROC) curve analysis. Results: We enrolled 307 patients, with a median preoperative BMI of 39.68 (35.68, 45.47) kg/m2. %EWL ≥ 50% was regarded as successful weight loss, and 76.55% of the patients lost their weight successfully. (Reviewer #1, comment #4) %EWL at 6 months and 5 years were positively correlated (P < 0.001). Further, the following linear equation could express the relationship: (%EWL5 years = 29.193 + 0.526 × %EWL6 months). %EWL ≥ 58.57% at 6 months was the best predictor of successful weight loss at 5 years after LSG (Reviewer #1, comment #5) (sensitivity, 73.62%; specificity, 73.61%; AUC value, 0.780). Internal verification of the prediction model revealed satisfactory results in terms of discrimination and calibration. Conclusion: In Chinese patients with BMI ≥ 32.5 kg/m2 who underwent LSG, %EWL at 6 months and 5 years were correlated. %EWL ≥ 58.57% at 6 months was a predictor of successful long-term weight loss.

15.
J Immunol Res ; 2022: 2932837, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35785034

RESUMO

Objective: To investigate the correlation between interleukin-27 and CXCL10 and other cytokines in pulmonary tuberculosis and to further explore the related miRNAs through bioinformatics. Methods: Collect the lesion tissue and peripheral blood of pulmonary tuberculosis patients and the peripheral blood of healthy controls. Immunohistochemical staining and qRT-PCR were used to observe the expression of interleukin-27, CXCL9, CXCL10, and CXCL11. Then, predict the key miRNA, qRT-PCR was used to verify the expression of miRNA in the peripheral blood and evaluated the correlation between them. Results: Both immunohistochemical staining and qRT-PCR indicated that the expressions of IL-27, CXCL9, CXCL10, and CXCL11 were significantly increased in tuberculosis patients, and IL-27 was significantly correlated with CXCL10 (r = 0.68). Key molecules such as has-let-7b-5p, has-miR-30a-3p, and has-miR-320b were screened out. Among them, has-let-7b-5p was significantly downregulated, and has-miR-30a-3p was significantly upregulated; they were related to interleukin-27 and CXCL10. Conclusion: Our data shows that interleukin-27 and CXCL10 are significantly related in pulmonary tuberculosis, and has-let-7b-5p and has-miR-30a-3p are also related to interleukin-27 and CXCL10. It laid the foundation for subsequently exploiting the potential biomarkers in tuberculosis disease.


Assuntos
Interleucina-27 , MicroRNAs , Tuberculose Pulmonar , Biomarcadores , Quimiocina CXCL10/genética , Biologia Computacional , Humanos , Interleucina-27/genética , MicroRNAs/genética , MicroRNAs/metabolismo , Tuberculose Pulmonar/genética
16.
Biomed Res Int ; 2022: 9127520, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35782060

RESUMO

Background: Phospholipase A2 receptor (PLA2R), located at the membrane of glomerular podocyte, is the major autoantigen of idiopathic membranous nephropathy (IMN), and its antibodies with a predominant IgG4 subclass lead to pathological lesions. Further studies could be performed to validate the clinical values of PLA2R-IgG, PLA2R-IgG4, and PLA2R-IgG4-to-IgG ratios, as ultrasensitive and quantitative immunoassays for PLA2R antibodies have been well established in our previous work. Methods: A cohort of 58 IMN patients, 30 of whom were followed from 3 to 42 months, was assessed for serum PLA2R-IgG and -IgG4 levels, and the ratio of PLA2R-IgG4/-IgG combined with relative clinicopathological indicators. Results: Serum PLA2R-IgG4 level was significantly correlated with glomerular PLA2R staining. In addition, it was strongly correlated with PLA2R-IgG and its ratio. PLA2R-IgG and -IgG4 levels were both correlated with high-density lipoprotein and erythrocyte sedimentation rates. The ratio at the first diagnosis can predict the remission, and its efficacy overmatched PLA2R-IgG4. In the survival curves, negative results for the ratio or PLA2R-IgG4 at the first diagnosis demonstrated higher remission rates. Conclusion: Serum PLA2R-IgG4 concentration may replace renal PLA2R immunohistochemistry in IMN diagnosis. We propose that the PLA2R-IgG4-to-IgG ratio and PLA2R-IgG4 could be novel indicators for remission prediction in clinical practice.


Assuntos
Glomerulonefrite Membranosa , Glomerulonefrite Membranosa/patologia , Humanos , Imunoensaio , Imunoglobulina G , Glomérulos Renais/patologia , Receptores da Fosfolipase A2
17.
Biomark Res ; 10(1): 46, 2022 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-35831833

RESUMO

T-cell lymphoma (TCL) is an aggressive and genetically heterogeneous malignancy with adverse clinical outcomes; thus, it is worth exploring biomarkers for risk stratification. Previous studies have demonstrated that transmembrane protein 244 gene (TMEM244) is ectopically expressed in Sézary syndrome (SS). In this study, the expression level of TMEM244 and its prognostic value for TCL patients was explored by analyzing RNA-seq data of two large datasets (GSE132550 and GSE113113) containing 129 TCL patients and 13 healthy individuals (HIs) from the Gene Expression Omnibus (GEO) database, the PRJCA002270 dataset containing 124 patients with T-cell acute lymphoblastic leukemia (T-ALL) from the BioProject database, and peripheral blood (PB) samples of 24 TCL and 29 T-ALL patients, as well as 11 normal CD3 + T-cells from our clinical center (JNU). The results suggested that TMEM244 was significantly up-regulated in TCL patients compared with normal CD3 + T-cells or T-ALL in the JNU, GSE132550 and GSE113113 datasets (P < 0.05). However, TMEM244 shows no expression in patients with T-ALL in the JNU-T-ALL and PRJCA002270 datasets. The receiver operating characteristic (ROC) curve analysis indicated that TMEM244 expression had a very high accuracy in diagnosing TCL compared with T-ALL (area under the curve (AUC): 99.4%; P < 0.001). Importantly, high TMEM244 expression was significantly associated with poor OS and shorter 5-year restricted mean survival time (RMST) in TCL patients, especially those treated with chemotherapy. In summary, TMEM244 is also expressed in other types of TCL besides SS, but not in T-ALL. High TMEM244 expression is associated with poor OS in TCL patients, which might be a novel biomarker for prognostic stratification in TCL patients and facilitate the design of novel therapies.

18.
Biomark Res ; 10(1): 47, 2022 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-35831911

RESUMO

Immunotherapy using programmed cell death 1 (PD1) inhibitors has shown great efficacy in colorectal cancer patients harboring mismatch-repair-deficient (dMMR) and microsatellite instability-high (MSI-H) alterations. We previously showed a negative correlation of zymogen granule protein 16 (ZG16) with programmed death-ligand 1 (PD-L1) expression in patients with colorectal cancer. However, how ZG16 regulates PD-L1 expression is unclear. In this study, we showed that ZG16 can directly bind to glycosylated PD-L1 through its lectin domain, leading to PD-L1 degradation. Mutations on the lectin domain of ZG16 largely inhibit the interaction between ZG16 and PD-L1. Importantly, ZG16 overexpression suppressed tumor growth in two syngeneic mouse models through blockage of PD-L1 expression in cancer cells meanwhile suppression of PD1 expression in T cells. We also showed that ZG16 could improve the effect of chemotherapy and may be delivered as a protein to serve as an immune checkpoint inhibitor to promote T-cell mediated immunity.

19.
Front Surg ; 9: 931109, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35832498

RESUMO

Objective: To explore the association between a modified Blumgart anastomosis technique and the operative time and surgical complications. Methods: This is a retrospective cohort study that analyzed the data of patients who underwent laparoscopic pancreaticoduodenectomy from January 2015 to March 2021. The primary outcome was to explore the association between the modified Blumgart anastomosis technique and operative time. Results: A total of 282 patients were enrolled. There were 177 cases of pancreatic duct-to-mucosa anastomosis in the traditional surgery group, and 105 cases of the modified three-step Blumgart anastomosis in the modified group. There were no statistically significant differences in the general and intraoperative characteristics found between the two groups (P > 0.05). The surgical method was an independent predictor of operative time. Overall complications postsurgery were less common in the modified group than in the traditional group. The incidence of postoperative pancreatic fistula was higher in the traditional group than in the modified group (45 cases (25.4%) and 11 cases (10.5%), respectively). Fourteen cases (7.9%) in the traditional group and four case (3.8%) in the modified group had postoperative pancreatic fistula of grades B + C. The two groups had statistically significant differences (P < 0.05). The results of the linear regression showed that the type of surgical method was associated with operation time (95% CI, -73.074 to -23.941, ß: -0.438, P < 0.001). Conclusion: This modified three-step Blumgart pancreaticojejunostomy was associated with the operation time.

20.
J Inflamm Res ; 15: 3783-3796, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35832830

RESUMO

Purpose: Neoadjuvant immunotherapy and chemotherapy (nICT) is an emerging hotspot that has been shown to be safe and feasible for locally advanced esophageal squamous cell carcinoma (LA-ESCC). This real-world study aimed to develop and validate a novel predictive model [integrative inflammatory and nutritional score (IINS)] in LA-ESCC patients receiving nICT to predict the pathologic complete response (pCR). Patients and Methods: Patients with LA-ESCC who received nICT followed by surgery from Jun 2019 to Dec 2021 were enrolled and randomly divided into two sets (7:3). Using least absolute shrinkage and selection operator (LASSO) logistic regression analysis, the IINS was constructed in LA-ESCC patients received nICT to predict pCR. A nomogram based on IINS for pCR prediction was generated in the training cohort and verified in the validation cohort. Results: Of the 285 enrolled LA-ESCC patients received nICT followed by radical resection, 84 (29.5%) patients achieved pCR. A predictive index of IINS based on 8 inflammatory and nutritional indicators was constructed using the LASSO model. According to the cutoff finder, patients were then stratified into two groups (high and low). The pCR rates were significantly higher in high-IINS group than in low-IINS group in both the training cohort (44.7% vs 17.4%, P < 0.001) and validation cohort (50.0% vs 13.3%, P < 0.001). The IINS [odds ratio (OR) = 0.237, 95% confidence interval (CI) = 0.117-0.480, P < 0.001] was an independent significant predictor for pCR in multivariate logistic analyses. The IINS-based nomogram showed an excellent discrimination for pCR prediction (C-indexes = 0.759 and 0.812 for training and validation cohorts, respectively). Conclusion: Pretreatment IINS is an independent predictor for pCR in LA-ESCC patients who are treated with nICT. To our knowledge, the IINS-based nomogram is the first model for pCR prediction and may serve as a simple and potential risk stratification model in LA-ESCC who are treated with nICT.

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