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1.
Nanotechnology ; 31(4): 045401, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31574496

RESUMO

Tuning the compositions and morphologies of catalysts is very important for the design of efficient formic acid oxidation reaction (FAOR) electrocatalysts. Herein, unique PtPdP dendritic nanoparticles (PtPdP DNs) with uniform size and open-pore structure are fabricated by a facile method, in which the Pd and P elements are simultaneously incorporated into Pt DNs. The prepared PtPdP DNs show enhanced catalytic activity and stability for FAOR. The improved electrocatalytic activity toward FAOR for the PtPdP DNs is mainly attributed to the synergic enhancement effect of the structural and compositional advantages, which jointly promote the electrocatalytic kinetics and thus enhance the electrocatalytic performance.

2.
Nanotechnology ; 31(2): 025401, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31546241

RESUMO

Design and synthesis of Pt-based nanocrystals with controlled structural diversity and complexity can potentially bring about multifunctional properties. In this work, we present a facile two-step strategy for the construction of the PtPdRh mesoporous octahedral nanocages (PtPdRh MONCs). This unique nanoarchitectonics rationally integrates multiple advantages (i.e. the octahedral shape, hollow cavity and mesoporous surface) into one catalyst, which facilitates the efficient utilization of noble metal atoms at both of the interior and exterior surfaces. As expected, the resultant PtPdRh MONCs could effectively catalyze the oxygen reduction reaction (ORR) under acidic conditions. The demonstrated ORR activity and catalytic durability are superior to the commercial Pt/C catalyst. The present study would provide a general guidance for architectural and compositional engineering of noble metal nanocrystals with desired functionalities and properties.

3.
J Nanosci Nanotechnol ; 20(4): 2567-2572, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492277

RESUMO

The crystal types of nanoparticles (NPs) are governed by many factors, such as temperature, pH, solvents, surface ligands, etc. In this work, ß-HgS and α-HgS NPs coated with captopril (Cap) have been synthesized conveniently in the same raw materials and similar methods just with different temperature at 4 °C and 50 °C, respectively. More interestingly, phase transition of HgS NPs from ß-crystal (cubic crystal) to α-crystal (hexagonal crystal) via rising temperature from 4 to 50 °C was confirmed by X-ray powder diffraction (XRD) and transmission electron microscopy (TEM). The size of α-HgS NPs grew to 6.7±1.3 nm in the process of phase transition, which was about twice of ß-HgS NPs (3.5±1.0 nm). Furthermore, compared with the obvious absorption peak of ß-HgS NPs at 688 nm, the absorption peak of α-HgS NPs had a giant blue-shifted to 470 nm in Ultraviolet and visible (UV-vis) spectra. In addition, the ß-HgS NPs had a fluorescence emission at 900 nm differing from α-HgS NPs without emission. The α-HgS NPs had three peaks at 215 nm, 251 nm and 318 nm, while the ß-HgS NPs had only a distinct peak at 215 nm in circular dichroism (CD) spectra. These results provide a novel insight for the controlling preparation of different crystal types of nanoparticles.

4.
Opt Express ; 27(21): 29581-29593, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31684217

RESUMO

We present a proposal to generate robust optomechanical entanglement induced by the blue-detuning laser and the mechanical gain in a double-cavity optomechanical system. We show that the stability of the system can be obtained by introducing a cavity mode driven by the red-detuning laser in the blue-detuning regime. In contrast to the red-detuning regime, we find that the entanglement in the blue-detuning regime is extremely robust to temperature. The cavity mode driven by the blue-detuning laser can control indirectly the optomechanical entanglement between mechanical resonator and cavity mode driven by the red-detuning laser. Moreover, the entanglement between two cavity modes without direct coupling can also be achieved in our system. Although the entanglement is weak, it is robust to temperature, and meanwhile, the optomechanical entanglement is hardly affected by the temperature when the damping rate of the mechanical oscillator is close to zero. Furthermore, the entanglement amplification at high temperature can be achieved by adjusting the mechanical gain appropriately. Our proposal provides an efficient way to achieve robust optomechanical entanglement in the blue-detuning regime and entanglement amplification in optomechanical system with mechanical gain.

5.
Biosci Biotechnol Biochem ; : 1-9, 2019 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-31680642

RESUMO

Previous studies have shown that miR-210-3p is involved in the development and progression of atherosclerosis, but its specific mechanisms are still unclear. This study aims to reveal the mechanism of miR-210-3p and its target genes in macrophage lipid deposition and inflammatory response, and provide new ideas for the treatment of atherosclerosis. We found miR-210-3p increased sharply in the first 12 h induced by higher doses of ox-LDL in THP-1 macrophages and then gradually decreased. MiR-210-3p mimic transfection inhibited lipid uptake and inflammatory cytokine production in ox-LDL-induced macrophages. By inhibiting IGF2/IGF2R, miR-210-3p suppressed the expression of fatty acid transcriptase CD36 and transcription factor NF-κB in ox-LDL-induced macrophages. In conclusion, miR-210-3p inhibits the expression of CD36 and NF-κB by inhibiting IGF2 / IGF2R, thereby reducing lipid accumulation and inflammatory response in ox-LDL-induced macrophages. Enhancing miR-210-3p expression may be a new strategy for the treatment of atherosclerosis.

6.
Medicine (Baltimore) ; 98(44): e17772, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31689841

RESUMO

RATIONALE: Pituicytomas are exceptional rare tumors in the sellar and suprasellar regions with clinical manifestations, such as headache, visual disturbance, hypopituitarism, and decreased libido. Unlike that of common pituitary adenoma, the association between pituicytoma and Cushing disease (CD) is extremely rare. There were only 6 reported cases till now. In the current study, we describe an unusual case of pituicytoma associated with severe CD with a recurrence-free follow-up period of 49 months. PATIENT CONCERNS: A 32-year-old woman was referred to our hospital with moon face, central obesity, and purple stripes on the lower limbs. DIAGNOSES: The plasma cortisol level was 1122 nmol/L. The low-dose dexamethasone suppression test failed to suppress plasma cortisol. This test provided evidence of nonpituitary-dependent CD. However, magnetic resonance imaging demonstrated a sellar mass measuring approximately 7.6 × 5.7 mm. The patient was diagnosed with pituitary microadenoma. Histopathological analysis of the tissue sections based on the findings from the immunohistochemical staining diagnosed it as pituicytoma. INTERVENTIONS: Transsphenoidal surgery was performed to remove the pituitary mass. OUTCOMES: Within 2 months postoperatively, the patient's blood pressure and cortisol level decreased gradually and normalized on the 6th month when other symptoms of CD also disappeared. The patient is presently free from recurrence 49 months after the initial diagnosis. LESSONS: Based on the postoperative remission, CD was caused by pituitary disorders. A reasonable assumption is that an extremely small coexisting adenoma was not detected by the surgeon and washed out during the dissection. Another possible explanation might be the mass effect caused by this intrasellar lesion.

7.
Radiother Oncol ; 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31668985

RESUMO

BACKGROUND AND PURPOSE: We used radiomic analysis to establish a radiomic signature based on anatomical magnetic resonance imaging (MRI) sequences and explore its effectiveness as a novel prognostic biomarker for skull base chordoma (SBC). MATERIALS AND METHODS: In this retrospective study, radiomic analysis was performed using preoperative axial T1 FLAIR, T2-weighted, and enhanced T1 FLAIR from a single hospital. The primary clinical endpoint was progression-free survival. A total of 1860 3-D radiomic features were extracted from manually segmented region of interest. Pearson correlation coefficient was used for feature dimensional reduction and a ridge regression-based Cox proportional hazards model was used to determine a radiomic signature. Afterwards, radiomic signature and nine other potential prognostic factors, including age, gender, histological subtype, dural invasion, blood supply, adjuvant radiotherapy, extent of resection, preoperative KPS, and postoperative KPS were analyzed to build a radiomic nomogram and a clinical model. Finally, we compared the nomogram with each prognostic factor/model by DeLong's test. RESULTS: A total of 148 SBC patients were enrolled, including 64 with disease progression. The median follow-up time was 52 months (range 4-122 months). The Harrell's concordance index of the radiomic signature was 0.745 (95% CI, 0.709-0.781) for the validation cohort, and its discrimination accuracy in predicting progression risk at 5 years in the same cohort was 82.4% (95% CI, 72.6-89.7%). CONCLUSIONS: The radiomics is a low-cost, non-invasive method to predict SBC prognosis preoperatively. Radiomic signature is a potential prognostic biomarker that may allow the individualized evaluation of patients with SBC.

8.
Medicine (Baltimore) ; 98(45): e17658, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31702617

RESUMO

Patients with idiopathic membranous nephropathy (IMN) can be categorized into phospholipase A2 receptor (PLA2R)-associated and non-PLA2R-associated cases, according to serum PLA2R antibody status. The present study aimed to determine whether clinical features differed between these.A total of 89 patients with IMN were retrospectively recruited for the present study. Serum PLA2R-Ab levels were determined by time-resolved fluoroimmunoassay. Furthermore, the relationship between serum PLA2R antibody levels and their responses to immunosuppressants among patients with a complete follow-up period, which was defined as at least 1 year, was analyzed.Among these enrollees, 71 (80.0%) patients were positive for serum PLA2R antibody. Furthermore, patients with PLA2R-associated IMN had significantly higher age (with vs without, 54.31 ±â€Š14.03 vs 46.67 ±â€Š13.30 years old; P = .04), proteinuria (4.32 ±â€Š1.84 vs 3.29 ±â€Š1.90 g/d, P = .039), and serum albumin (25.33 ±â€Š9.60 vs 31.38 ±â€Š9.52 g/L, P = .019), but had lower serum immunoglobulin G (6.83 ±â€Š2.89 vs 8.72 ±â€Š2.95 g/L, P = .016) and erythrocyte sedimentation rate (47.31 ±â€Š32.11 vs 26.33 ±â€Š27.94, P = .013), when compared to IMN patients without PLA2R. Furthermore, IMN patients without PLA2R exhibited a better response to immunosuppressants, when compared to patients with PLA2R-associated IMN (without vs with, 66.7% vs 62.5% at 6 months and 100% vs 87.5% at 12 months), but the difference was not statistically significant.Patients with PLA2R-associated IMN had higher disease severity than IMN patients without PLA2R. Furthermore, PLA2R negative patients had a better response to immunosuppressive therapies than PLA2R-positive patients, but the difference was not statistically significant.

9.
Appl Opt ; 58(27): 7609-7614, 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31674416

RESUMO

This paper proposes an accommodative intraocular lens (IOL), which consists of a two-element Alvarez lens and an aspheric lens for changing focal power and refractive power, respectively. The four-freeform-surface Alvarez lens is optimized for a multiple field of view; further, the aspheric lens also corrects the aberrations induced by the corneal asphericity of the human eye over the whole range of accommodation. A simulation using optical design software demonstrates its excellent performance in that the values of the modulation transfer function at 100 cycles/mm all reach ∼0.4 with a ±5° field of view for 3 and 5 mm pupils.

10.
Sci Rep ; 9(1): 15839, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31676878

RESUMO

Crop production, including mushroom farming, may cause significant changes to the underlying substrates which in turn, can influence crop quality and quantity during subsequent years. Here in this study, we analyzed the production of the medicinal mushroom Ganoderma lingzhi and the associated soil microbial communities and soil chemical features over 24 months from April 2015 to April 2017. This Basidiomycete mushroom, known as Lingzhi in China, is commonly found on dead trees and wood logs in temperate and subtropical forests. Its economic and medicinal importance have propelled the development of a diversity of cultivation methods. The dominant method uses wood logs as the main substrate, which after colonization by Lingzhi mycelia, are buried in the soil to induce fruiting. The soil microbial communities over the 24 months were analyzed using the Illumina HiSeq platform targeting a portion of the bacterial 16S rRNA gene and the fungal internal transcribed spacer 1 (ITS1). Overall, a significant reduction of Lingzhi yield was observed over our experimentation period. Interestingly, temporal changes in soil microbial compositions were detected during the 24 months, with the fungal community showing more changes than that of bacteria in terms of both species richness and the relative abundance of several dominant species after each fruiting. The soil chemical features also showed significant changes, with decreasing soil nitrogen and phosphorus concentrations and increasing soil pH and iron content after each fruiting. We discuss the implications of our results in sustainable Lingzhi production in soil.

11.
Chemosphere ; 240: 124891, 2019 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-31574442

RESUMO

We investigated the effects of endophytes inoculation on ecological factors such as root morphology, rhizosphere soil properties, heavy metal speciation, and rhizosphere and endophytic bacterial communities and their role on phytoremediation. Indian mustards were grown for two months in V-contaminated soil with three treatments (control, inoculation with Serratia PRE01 or Arthrobacter PRE05). Inoculation with PRE01 and PRE05 increased organic matter content by 6.94% and 4.6% respectively and significantly increased bioavailability of heavy metals in rhizosphere soils. Despite the endophyte inocula failed to flourish as stable endophytes, they significantly affected the specific composition and diversity of endophytic bacterial communities in roots, with no significant effect on rhizosphere bacterial communities. The test strains could greatly increase plant growth promotion-related biomarkers in the endosphere, especially those associated with Pseudomonas and Microbacterium genera. PICRUSt analysis predicted high relative abundances of functional genes related to environmental information processing especially in the endophytic microbiota. More biomass production (12.0%-17.4%) and total metals uptake (24.2%-32.0%) were acquired in inoculated treatments. We conclude that endophyte PRE01 or PRE05 inoculation could effectively enhance phytoremediation of V-contaminated soil by improving the rhizosphere and endosphere microecology without causing any ecological damage.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31583908

RESUMO

Cerebral infarction is a leading cause of death, which calls for effective prevention and treatment. Transplant of neural stem cells (NSCs) is a potential therapeutic treatment to cerebral infarction although its efficacy still needs to be improved. Overexpression of Hypoxia-inducible factor 1α (HIF-1α) has been shown to enhance the protective effects of stem cell transplant on cerebral infarction. The expression of HIF-1α is predicted to be regulated by miR-155-5p. Therefore, we regulated the expression of miR-155-5p in NSCs and evaluated the effects of miR-155-5p-regulated NSC transplant on cerebral infarction. We inhibited miR-155-5p expression in NSCs by overexpressing miR-155-5p inhibitor. HIF-1α expression, cell viability and the expression of apoptosis markers were examined. We established the middle cerebral artery occlusion (MCAO) rat model, and the infarct volume, neurobehavioral outcomes, inflammation and oxidative stress were evaluated after NSC transplant. miR-155-5p directly targeted HIF-1α and negatively regulated its expression. Inhibition of miR-155-5p enhanced cell viability and prevented cell apoptosis. Transplant of miR-155-5p-inhibited NSCs significantly decreased infarct volume, improved neurobehavioral outcomes of MCAO rats. Transplant of miR-155-5p-inhibited NSCs significantly inhibited inflammation and oxidative stress. Inhibition of miR-155-5p in NSCs resulted in enhanced protection against cerebral infarction after NSC transplant.

13.
Chaos ; 29(9): 093118, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31575156

RESUMO

In exploring oscillator synchronization, a general observation is that as the oscillators become nonidentical, e.g., introducing parameter mismatch among the oscillators, the propensity for synchronization will be deteriorated. Yet in realistic systems, parameter mismatch is unavoidable and even worse in some circumstances, the oscillators might follow different types of dynamics. Considering the significance of synchronization to the functioning of many realistic systems, it is natural to ask the following question: Can synchronization be achieved in networked oscillators of clearly different parameters or dynamics? Here, by the model of networked chaotic oscillators, we are able to demonstrate and argue that, despite the presence of parameter mismatch (or different dynamics), stable synchronization can still be achieved on symmetric complex networks. Specifically, we find that when the oscillators are configured on the network in such a way that the symmetric nodes have similar parameters (or follow the same type of dynamics), cluster synchronization can be generated. The stabilities of the cluster synchronization states are analyzed by the method of symmetry-based stability analysis, with the theoretical predictions in good agreement with the numerical results. Our study sheds light on the interplay between symmetry and cluster synchronization in complex networks and give insights into the functionalities of realistic systems where nonidentical nonlinear oscillators are presented and cluster synchronization is crucial.

14.
J Cell Mol Med ; 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31576658

RESUMO

Increasing studies have confirmed that abnormally expressed microRNAs (miRNAs) take part in the carcinogenesis as well as the aggravation of hepatocellular carcinoma (HCC). However, little information is currently available about miR-1914 in HCC. Here, we first confirmed that miR-1914 inhibition in HCC cell lines and tumour specimens correlates with tumour size and histological grade. In a series of functional experiments, miR-1914 inhibited tumour proliferation and colony formation, resulting in cell cycle arrest and increased apoptosis. Moreover, miR-1914 mediated its functional effects by directly targeting GPR39 in HCC cells, leading to PI3K/AKT/mTOR repression. Restoring GPR39 expression incompletely counteracted the physiological roles of miR-1914 in HCC cells. In addition, down-regulation of AKT phosphorylation inhibited the effects of miR-1914 in HCC. Furthermore, the overexpression of lncRNA DUXAP10 negatively correlated with the expression of miR-1914 in HCC; thus, lncRNA DUXAP10 regulated miR-1914 expression and modulated the GPR39/PI3K/AKT-mediated cellular behaviours. In summary, the present study demonstrated for the first time that lncRNA DUXAP10-regulated miR-1914 plays a functional role in inhibiting HCC progression by targeting GPR39-mediated PI3K/AKT/mTOR pathway, and this miRNA represents a novel therapeutic target for patients with HCC.

15.
Chemistry ; 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31599996

RESUMO

Realization of water electrolysis on the basis of highly active, cost-effective electrocatalysts is significant yet challenging for achieving sustainable hydrogen production from water. Herein, N-doped-Ni3S2/N-doped-MoS2 1D heteronanowires supported by Ni foam (N-Ni3S2/N-MoS2/NF) are readily synthesized via a chemical transformation strategy by using NiMoO4 nanowires array growth on Ni foam (NiMoO4/NF) as the starting material. With the in-situ generation of Ni3S2/MoS2 heterointerfaces within nanowires and the incorporation of N-anion atoms, an extraordinary hydrophilic nature, abundant well-exposed active sites, and optimal reaction dynamics for both oxidation and reduction of water are obtained. Attributed to the above nature superiorities, the as-converted N-Ni3S2/N-MoS2/NF exhibit highly efficient electrocatalytic activities for both hydrogen and oxygen evolution reactions under alkaline conditions. The superior bifunctional properties of the N-Ni3S2/N-MoS2/NF enable it to effcctively catalyze the overall water splititng reaction.

16.
Trials ; 20(1): 600, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31640763

RESUMO

BACKGROUND: There is a growing debate on the relationship between health-related quality of life (HRQoL) and patient survival which has been going on for the last few decades. The greatest wish of clinicians is to extend the latter while improving the former. Following neck dissection of early-stage oral carcinoma, "shoulder syndrome" appears due to traction of the accessory nerve during removal of level IIb, which greatly affects patient quality of life. Since occult metastasis in level IIb of early-stage oral carcinoma is extremely low, some surgeons suggest that level IIb can be exempt from dissection to improve the HRQoL. However, other surgeons take the opposite view, and thus there is no consensus on the necessity of IIb dissection in T1-2N0M0 oral squamous cell carcinoma (OSCC). METHODS: We designed a parallel-group, randomized, non-inferiority trial that is supported by Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China. We will enroll 522 patients with early oral carcinoma who match the inclusion criteria, and compare differences in 3-year overall survival, progression-free survival (PFS) and HRQoL under different interventions (retention or dissection of level IIb). The primary endpoints will be tested by means of two-sided log-rank tests. Analysis of overall and progression-free survival will be performed in subgroups that were defined according to stratification factors with the use of univariate Cox analysis. In addition, we will use post-hoc subgroup analyses on the basis of histological factors that were known to have effects on survival, such as death of invasion of the primary tumor. To evaluate HRQoL, we will choose the Constant-Murley scale to measure shoulder function. DISCUSSION: Currently, there are no randomized controlled trials with large sample sizes on the necessity of IIB dissection in T1-T2N0M0 OSCC. We designed this noninferiority RCT that combines survival rate and HRQoL to assess the feasibility of IIb neck dissection. The result of this trial may guide clinical practice and change the criteria of how early-stage oral cancer is managed. The balance between survival and HRQoL in this trial is based on early-stage breast cancer treatment and may provide new ideas for other malignancies. TRIAL REGISTRATION: Chinese Clinical Trial Registry, ChiCTR1800019128 . Registered on 26 October 2018.

17.
Artigo em Inglês | MEDLINE | ID: mdl-31647434

RESUMO

Zero-shot learning aims to recognize objects which do not appear in the training dataset. Previous prevalent mapping-based zero-shot learning methods suffer from the projection domain shift problem due to the lack of image classes in the training stage. In order to alleviate the projection domain shift problem, a deep unbiased embedding transfer (DUET) model is proposed in this paper. The DUET model is composed of a deep embedding transfer (DET) module and an unseen visual feature generation (UVG) module. In the DET module, a novel combined embedding transfer net which integrates the complementary merits of the linear and nonlinear embedding mapping functions is proposed to connect the visual space and semantic space. What's more, the end-to-end joint training process is implemented to train the visual feature extractor and the combined embedding transfer net simultaneously. In the UVG module, a visual feature generator trained with a conditional generative adversarial framework is used to synthesize the visual features of the unseen classes to ease the disturbance of the projection domain shift problem. Furthermore, a quantitative index, namely the score of resistance on domain shift (ScoreRDS), is proposed to evaluate different models regarding their resistance capability on the projection domain shift problem. The experiments on five zero-shot learning benchmarks verify the effectiveness of the proposed DUET model. As demonstrated by the qualitative and quantitative analysis, the unseen class visual feature generation, the combined embedding transfer net and the end-to-end joint training process all contribute to alleviating projection domain shift in zero-shot learning.

18.
Environ Res ; 179(Pt A): 108778, 2019 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-31629946

RESUMO

BACKGROUND: Emerging evidence from animals indicates that oxidative stress plays a crucial role in the effects of phthalate exposure on male reproductive dysfunctions, which has never been thoroughly explored in humans. OBJECTIVE: To explore the potential mediating role of oxidative stress in the association of phthalate exposure with semen quality among 1034 Chinese men. METHOD: Repeated urine samples gathered from the male partners of sub-fertile couples were analyzed for 3 oxidative stress markers [8-hydroxy-2-deoxyguanosine (8-OHdG), 8-iso-prostaglandin F2α (8-isoPGF2α) and 4-hydroxy-2-nonenal-mercapturic acid (HNE-MA)], using a liquid chromatography-tandem mass spectrometry. Multivariate regression models were constructed to evaluate the associations of urinary oxidative stress markers with urinary phthalate metabolites and semen quality. We also explored the potential mediation effects by oxidative stress markers. RESULTS: Significantly positive dose-dependent relationships were observed between each individual phthalate metabolite and all analyzed oxidative stress markers (all p for trend<0.05), except for monoethyl phthalate (MEP) in relation to HNE-MA. Additionally, significantly or suggestively inverse dose-dependent relationships were exhibited between urinary 8-isoPGF2α and sperm concentration (p for trend = 0.05), and between urinary 8-OHdG and percent of normal sperm morphology (p for trend = 0.01). Mediation analysis showed that urinary 8-isoPGF2α suggestively mediated 12% of the inverse association between monobutyl phthalate (MBP) and sperm concentration, and that urinary 8-OHdG suggestively mediated 32% of the inverse association of MEP with percent of normal sperm morphology (both p < 0.10). CONCLUSIONS: Although further investigations are required, our results suggest that oxidative stress may play a mediating role in the effects of phthalate exposure on impaired semen quality.

19.
Cancer Med ; 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31642612

RESUMO

Pancreatic cancer (PC) is a highly malignant tumor with increased morbidity and mortality, which is difficult to diagnose and cure in the clinic. Through secreting exosomes containing biological molecules, including diverse RNAs and proteins, bone marrow mesenchymal stem cells (BM-MSCs) influence the immunity, inflammation, tumor environment, and cancer metastasis. In this study, low expression of miRNA-1231 (miR-1231) in exosomes derived from the peripheral blood was significantly correlated with the TNM stage of PC, suggesting the potential inhibitory effect of exosomal miR-1231 on PC occurrence and development. The proliferation, migration, invasion, and adhesion to the matrix of PC cells BxPC-3 and PANC-1 were negatively regulated by exosomes derived from the supernatants of BM-MSCs that transfected with miR-1231 oligonucleotides. Simultaneously, tumor growth in vivo was seriously restrained in BALB/C nude mice by tail vein injection with exosomes originated from BM-MSCs that transfected with miR-1231 mimics. The exosomes extracted from BM-MSCs with high level of miR-1231 inhibit the activity of PC, providing the potential application for developing new and efficient medicine for cancer therapy, especially for PC treatment. The exosomal miR-1231 of peripheral blood may also be a potential indicator for PC diagnosis in the future.

20.
Hum Gene Ther Methods ; 30(5): 184-193, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31618139

RESUMO

Cerebral infarction is a leading cause of death, which calls for effective prevention and treatment. Transplant of neural stem cells (NSCs) is a potential therapeutic treatment to cerebral infarction although its efficacy still needs to be improved. Overexpression of hypoxia-inducible factor 1α (HIF-1α) has been shown to enhance the protective effects of stem cell transplant on cerebral infarction. The expression of HIF-1α is predicted to be regulated by miR-155-5p. Therefore, we regulated the expression of miR-155-5p in NSCs and evaluated the effects of miR-155-5p-regulated NSC transplant on cerebral infarction. We inhibited miR-155-5p expression in NSCs by overexpressing miR-155-5p inhibitor. HIF-1α expression, cell viability, and the expression of apoptosis markers were examined. We established the middle cerebral artery occlusion (MCAO) rat model, and the infarct volume, neurobehavioral outcomes, inflammation, and oxidative stress were evaluated after NSC transplant. miR-155-5p directly targeted HIF-1α and negatively regulated its expression. Inhibition of miR-155-5p enhanced cell viability and prevented cell apoptosis. Transplant of miR-155-5p-inhibited NSCs significantly decreased infarct volume and improved neurobehavioral outcomes of MCAO rats. Transplant of miR-155-5p-inhibited NSCs significantly inhibited inflammation and oxidative stress. Inhibition of miR-155-5p in NSCs resulted in enhanced protection against cerebral infarction after NSC transplant.

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