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2.
Chem Commun (Camb) ; 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31909399

RESUMO

Control of magnetic performances of molecular magnets is essential but few efforts have been documented. A green and efficient sonication assisted synthesis of a new heterometallic diruthenium(ii,iii) carbonate, Na[Ni(H2O)4Ru2(CO3)4]·3H2O (1), was carried out by self-assembling in aqueous solution. Compound 1 exhibits spin-glass behavior below ∼5.0 K, and a systematic investigation of the ultrasonic irradiation influence on the powder samples reveals that their coercivity increases from 50 Oe to 743 Oe with the control of ultrasonic power under appropriate conditions.

3.
Proc Natl Acad Sci U S A ; 116(51): 25524-25529, 2019 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-31792191

RESUMO

Strain describes the deformation of a material as a result of applied stress. It has been widely employed to probe transport properties of materials, ranging from semiconductors to correlated materials. In order to understand, and eventually control, transport behavior under strain, it is important to quantify the effects of strain on the electronic bandstructure, carrier density, and mobility. Here, we demonstrate that much information can be obtained by exploring magnetoelastoresistance (MER), which refers to magnetic field-driven changes of the elastoresistance. We use this powerful approach to study the combined effect of strain and magnetic fields on the semimetallic transition metal dichalcogenide [Formula: see text] We discover that WTe2 shows a large and temperature-nonmonotonic elastoresistance, driven by uniaxial stress, that can be tuned by magnetic field. Using first-principle and analytical low-energy model calculations, we provide a semiquantitative understanding of our experimental observations. We show that in [Formula: see text], the strain-induced change of the carrier density dominates the observed elastoresistance. In addition, the change of the mobilities can be directly accessed by using MER. Our analysis also reveals the importance of a heavy-hole band near the Fermi level on the elastoresistance at intermediate temperatures. Systematic understanding of strain effects in single crystals of correlated materials is important for future applications, such as strain tuning of bulk phases and fabrication of devices controlled by strain.

4.
Adv Healthc Mater ; 8(24): e1901301, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31763779

RESUMO

Due to their excellent size, designability, and outstanding targeted antibacterial effects, nanoparticles have become a potential option for controlling oral biofilm-related infections. However, the formation of an oral biofilm is a dynamic process, and factors affecting the performance of antibiofilm treatments are complex. As such, when examining the existing literature on the antibiofilm effects of nanoparticles, attention should be paid to the specific mechanisms of action at different stages of oral biofilm formation, as well as relevant influencing factors, in order to achieve an objective and comprehensive evaluation. This review is intended to detail the antibacterial mechanisms of nanoparticles during the four stages of the formation of oral biofilms: 1) acquired film formation; 2) bacterial adhesion; 3) early biofilm development; and 4) biofilm maturation. In addition, factors influencing the antibiofilm properties of nanoparticles are summarized from the aspects of nanoparticles themselves, biofilm models, and host factors. The limitations of current research and possible trends for future research are also discussed. In summary, nanoparticles are a promising antioral biofilm strategy. It is hoped that this review can serve as a reference and inspire ideas for further research on the application of nanoparticles for effectively targeting and treating oral biofilms.

5.
Ther Adv Med Oncol ; 11: 1758835919874651, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31579114

RESUMO

Background: Accumulating evidence has highlighted the crucial role of long noncoding RNAs (lncRNAs) in the tumorigenesis of gastric cancer (GC), which is the most common gastrointestinal malignancy. The present study aimed to identify the capacity of lncRNA LINC01419 (LINC01419) in GC progression, with the potential mechanism explored. Methods: Highly expressed lncRNAs were identified by in silico analysis, with the LINC01419 expression in GC tissues measured using reverse transcription-quantitative PCR (RT-qPCR). The GC cells were subsequently transfected with siRNA against LINC01419 or Rapamycin (the inhibitor of the mTOR pathway), or both, in order to measure cell migration and invasion in vitro as well as tumor growth and metastasis in vivo. Moreover, the expression of PI3K/Akt1/mTOR pathway-associated factors was determined. Results: LINC01419, highly expressed in GC samples of the Gene Expression Omnibus database, was observed to be markedly upregulated in GC tissues. Moreover, LINC01419 silencing, or PI3K/Akt1/mTOR pathway inhibition, exhibited an inhibitory role in GC cell migration and invasion in vitro, coupled with promoted cell autophagy in vitro, and inhibited tumor growth and metastasis in vivo. It was also revealed that LINC01419 silencing blocked the PI3K/Akt1/mTOR pathway, as proved by decreased extents of Akt1 and mTOR phosphorylation. Conclusions: In conclusion, LINC01419 inhibition may suppress GC cell invasion and migration, and promote autophagy via inhibition of the PI3K/Akt1/mTOR pathway. This provides significant theoretical basis and possibilities for further elucidation of the molecular mechanism of GC and finding new molecular-targeted therapeutic regimens.

6.
Life Sci ; 235: 116863, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31513817

RESUMO

AIMS: To determine whether dimethyl fumarate (DMF) can protect against lipopolysaccharide (LPS) -induced myocardial injury. MAIN METHODS: H9c2 cells pretreated with or without DMF were stimulated with LPS. Cell viability and apoptosis were evaluated. Nrf2 and HO-1 expression were detected using Western blotting. Mitochondrial morphology, mitochondrial superoxide production were observed using confocal microscope. Mitochondrial respiration function was measured using Seahorse bioanalyzer. KEY FINDINGS: (1) The cell viability decreased, LDH release and apoptosis increased in LPS- challenged H9c2 cells. DMF pretreatment brought a higher cell viability, and a lower LDH leakage and apoptosis than those of LPS group (P < 0.01). (2) DMF pretreatment resulted in an increased Nrf2 and HO-1 expression, and enhanced nuclear Nrf2 level in LPS-challenged cells (P < 0.01). (3) Nrf2-siRNA could inhibit DMF-induced enhancement of HO-1 expression and cell viability, and partly abolish DMF-induced reduction of LDH leakage and apoptosis. (4) ERK1/2 inhibitor PD98059 could not only prevent the DMF-induced enhancement of nuclear Nrf2 and HO-1, but also inhibit DMF-induced increase in cell viability. (5) Compared with LPS-challenged cells, DMF pretreatment caused a lower production of mitochondrial superoxide and a higher mitochondrial membrane potential, which could be abolished by Nrf2-siRNA. (6) DMF could attenuate LPS-induced mitochondrial fragmentation and improve mitochondrial respiration function by enhancement of the oxygen consumption rate of basal respiration and ATP production in LPS-challenged cells (P < 0.01). SIGNIFICANCE: DMF protects cardiomyocytes against LPS-induced damage. ERK1/2-dependent activation of Nrf2/HO-1 pathway is responsible for DMF-induced cardioprotection via reduction of oxidative stress, improvement of mitochondrial morphology and energy metabolism.


Assuntos
Fumarato de Dimetilo/farmacologia , Mitocôndrias/efeitos dos fármacos , Miócitos Cardíacos/efeitos dos fármacos , Fator 2 Relacionado a NF-E2/metabolismo , Transdução de Sinais/efeitos dos fármacos , Trifosfato de Adenosina/biossíntese , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Fumarato de Dimetilo/antagonistas & inibidores , Flavonoides/farmacologia , Heme Oxigenase-1/metabolismo , Humanos , L-Lactato Desidrogenase/metabolismo , Lipopolissacarídeos/efeitos adversos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Mitocôndrias/metabolismo , Miócitos Cardíacos/metabolismo , Fator 2 Relacionado a NF-E2/antagonistas & inibidores , Consumo de Oxigênio/efeitos dos fármacos , Substâncias Protetoras/farmacologia , RNA Interferente Pequeno/farmacologia , Superóxidos/metabolismo
7.
Acta Crystallogr C Struct Chem ; 75(Pt 9): 1280-1285, 2019 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-31484817

RESUMO

By employing the semi-rigid multidentate carboxylic acid ligand 4,4',4''-{[(2,4,6-trimethylbenzene-1,3,5-triyl)tris(methylene)]tris(oxy)}tribenzoic acid (denoted H3L), a new lanthanum coordination polymer, namely poly[[bis(dimethylformamide)(µ6-4,4',4''-{[(2,4,6-trimethylbenzene-1,3,5-triyl)tris(methylene)]tris(oxy)}tribenzoato)lanthanum(III)] dimethylformamide tetrasolvate 0.25-hydrate], {[La(C33H27O9)(C3H7NO)2]·4C3H7NO·0.25H2O}n or {[La(L)(DMF)2]·4(DMF)·0.25(H2O)}n (DMF is dimethylformamide) (1), was prepared and characterized by single-crystal X-ray diffraction, elemental analysis, thermogravimetric analysis, IR spectroscopy and photoluminescence studies. The asymmetric unit contains one LaIII cation, one anionic L3- ligand, two coordinated DMF molecules, four free DMF molecules and one-quarter of a free water molecule. Compound 1 possesses (3,6)-connected two-dimensional kgd topology sheets consisting of secondary building units of La2 clusters and L3- ligands, which further stack into three-dimensional supramolecular networks through π-π interactions. Compound 1 exhibits a photoluminescence emission at room temperature, with a peak at 410 nm, owing to a ligand-centred excited state.

8.
New Phytol ; 224(4): 1512-1517, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31418867

RESUMO

A widely observed pollinator decline around the world has led to the prediction that terrestrial ecosystems could be disrupted as plant pollination suffers, but declining pollination success has not been tested rigorously in wild plants, and it still remains unclear how pollination success of plant species responds differently in the context of pollinator decline. By viewing the number of seeds per pod as a quantitative measure of successful pollination, we examined seed pods in 4637 herbarium specimens of 109 obligately outcrossing legumes collected over the past century. We found that only 13 species showed significant temporal change with nine of those as an increase. None of the three subfamilies of legumes showed a consistent trend, and the subfamily Papilionoideae with the most specialized flowers, had increasing seed number per pod more often than decreasing. We conclude that legume pollination in China shows no sign of disruption and the effects of plant-pollinator disruption may be more complicated than simplistic predictions have allowed.

9.
Zhongguo Zhen Jiu ; 39(6): 599-603, 2019 Jun 12.
Artigo em Chinês | MEDLINE | ID: mdl-31190495

RESUMO

OBJECTIVE: To compare the clinical effect differences between electroacupuncture (EA) combined with penetrating moxibustion and the biological feedback training of pelvic floor muscle for postpartum stress urinary incontinence (SUI). METHODS: Sixty patients of SUI who had delivery 42 days ago were randomly divided into an observation group and a control group, 30 cases in each one. The observation group was treated with EA and penetrating moxibustion. EA was applied at Ciliao (BL 32) and Huiyang (BL 35), combined with acupuncture at Qihai (CV 6), Zhongji (CV 3), Zigong (EX-CA 1), Zusanli (ST 36) and Sanyinjiao (SP 6); penetrating moxibustion was performed on abdomen and lumbosacral area. The control group was treated with biological feedback training of pelvic floor muscle. Both the groups were treated once every other day, 3 times per week for continuous 6 weeks. The International Consultation on Incontinence Questionnaire-Short Form (ICI-Q-SF), 1 h urinal pad test and pelvic floor muscle strength were tested before and after treatment; the efficacy was evaluated after treatment and at 6-month follow-up visit. RESULTS: Compared before treatment, the ICI-Q-SF score and 1 h urine leakage were significantly reduced after treatment in the two groups (P<0.01), and the reduction in the observation group was superior to that in the control group (P<0.05). Compared before treatment, the pelvic muscle strength of muscle fibers Ⅰand Ⅱ were significantly increased after treatment in the two groups (P<0.01), and the differences between the two groups had no statistical significance (P>0.05). After treatment, the cured rate and total effective rate were 70.0% (21/30) and 96.7% (29/30) in the observation group, which were superior to 33.3% (10/30) and 70.0% (21/30) in the control group (P<0.01); in the 6-month postpartum period, the cured rate and total effective rate were 63.3% (19/30) and 93.3% (28/30) in the observation group, which were superior to 30.0% (9/30) and 66.7% (20/30) in the control group (P<0.05). CONCLUSION: EA combined with penetrating moxibustion could improve the urinary control ability, relieve the symptoms of urinary incontinence and have a better long-term effect in patients with postpartum SUI, which is superior to biological feedback training of pelvic floor muscle.


Assuntos
Eletroacupuntura , Moxibustão , Complicações na Gravidez , Incontinência Urinária por Estresse , Feminino , Humanos , Período Pós-Parto , Gravidez , Complicações na Gravidez/terapia , Resultado do Tratamento , Incontinência Urinária por Estresse/terapia
10.
Theranostics ; 9(7): 2017-2035, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31037154

RESUMO

Recent studies have demonstrated that bone marrow mesenchymal stem cells (BMSCs) protect the injured neurons of spinal cord injury (SCI) from apoptosis while the underlying mechanism of the protective effect of BMSCs remains unclear. In this study, we found the transfer of mitochondria from BMSCs to injured motor neurons and detected the functional improvement after transplanting. Methods: Primary rat BMSCs were co-cultured with oxygen-glucose deprivation (OGD) injured VSC4.1 motor neurons or primary cortical neurons. FACS analysis was used to detect the transfer of mitochondria from BMSCs to neurons. The bioenergetics profiling of neurons was detected by Extracellular Flux Analysis. Cell viability and apoptosis were also measured. BMSCs and isolated mitochondria were transplanted into SCI rats. TdT-mediated dUTP nick end labelling staining was used to detect apoptotic neurons in the ventral horn. Immunohistochemistry and Western blotting were used to measure protein expression. Re-myelination was examined by transmission electron microscope. BBB scores were used to assess locomotor function. Results: MitoTracker-Red labelled mitochondria of BMSCs could be transferred to the OGD injured neurons. The gap junction intercellular communication (GJIC) potentiator retinoid acid increased the quantity of mitochondria transfer from BMSCs to neurons, while GJIC inhibitor 18ß glycyrrhetinic acid decreased mitochondria transfer. Internalization of mitochondria improved the bioenergetics profile, decreased apoptosis and promoted cell survival in post-OGD motor neurons. Furthermore, both transplantation of mitochondria and BMSCs to the injured spinal cord improved locomotor functional recovery in SCI rats. Conclusions: To our knowledge, this is the first evidence that BMSCs protect against SCI through GJIC to transfer mitochondrial to the injured neurons. Our findings suggested a new therapy strategy of mitochondria transfer for the patients with SCI.

11.
Dalton Trans ; 48(23): 8039-8043, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31080985

RESUMO

The synthesis of antiferromagnets (AFMs) has attracted extensive attention in the area of magnetic devices, such as spintronics and memory devices. Following our initial work on the employment of the homo-valent diruthenium(iii,iii) paddle wheel species with high spin states (S = 2) as building blocks and active components for the construction of molecule-based magnetic materials, the reaction of mixed-valent diruthenium(ii,iii) phosphates Ru2(H2PO4)2(H1.5PO4)2(H2O)2·2H2O (1) with H2O2, Cu2+ and ACl (A = K, Rb and Cs) in aqueous solution led to the formation of heterometallic copper diruthenium(iii,iii) phosphates A2[Cu(H2O)4Ru2(HPO4)2(PO4)2(H2O)2]·nH2O [A = K (2); A = Rb and n = 2 (3); A = Cs and n = 3 (4)]. The compounds consist of chain structures in which each octahedral environment of Cu(H2O)42+ bonds to two [Ru(HPO4)2(PO4)2]2- units in a trans manner, forming a ferrimagnetic negative chain {Cu(H2O)4Ru2(HPO4)2(PO4)2(H2O)2}n2n-, exhibiting anomalous magnetic properties. Long-range ordering temperatures, Tc, were observed around 10 K, and the control of the chain structured antiferromagnets exhibiting step-like hysteresis loops was successfully achieved through counterion tuning by replacing K with either Rb or Cs.

12.
ACS Sens ; 4(6): 1612-1618, 2019 06 28.
Artigo em Inglês | MEDLINE | ID: mdl-31099246

RESUMO

Dead cells always accompany with live cells in vivo and in cell culture. It is important to distinguish dead cells from live cells in various biological studies. Currently, the probes for dead cells are mainly nucleic acid-intercalators, most of which have low affinity and potential toxicity to live cells. In this work, we report a novel aptameric probe (Ch4-1) for the first time, which binds cell nuclei with high affinity (apparent Kd = 6.65 ± 3.40 nM). Ch4-1 was generated by Cell-SELEX process, it was identified to target nucleoproteins in cell nuclei. As an oligonucleotide, Ch4-1 cannot penetrate the integrated cell membrane; therefore, it only binds to dead cells rather than live cells. Compared with traditional DNA-targeting nuclear dyes, Ch4-1 possesses a high affinity to the nucleus, no toxicity to live cells, and can be easily labeled with different fluorescent dyes. It was demonstrated to serve as a probe for distinguishing dead cells from live cells in apoptosis assay, as well as for the nuclear staining of tissue sections.

13.
Ying Yong Sheng Tai Xue Bao ; 30(4): 1199-1206, 2019 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-30994280

RESUMO

To improve water and nitrogen (N) fertilizer use efficiency, increase sustainable agricultural production, and explore the appropriate substitution level of inorganic N fertilizer by organic fertilizer in the semi-arid region of the western Loess Plateau, a three-year field experiment was conducted at the Dingxi Agri-ecological Station in 2016-2018. We examined the effects of commercial organic fertilizer substitution on maize grain yield as well as the use efficiency of water and N fertilizer under plastic film fully mulched ridge-furrow in dryland. There were six fertilizer treatments: T1, N fertilizer without organic fertilizer; T2, substitution 50% inorganic-N with organic-N; T3, substitution 37.5% inorganic-N with organic-N; T4, substitution 25% inorganic-N with organic-N; T5, substitution 12.5% inorganic-N with organic-N; and CK, no N fertilizer. The results showed that treatments of commercial organic fertilizer substitution (T2-T5) had higher grain yield and water and N efficiency than that in T1 treatment in dry year under 200 kg N·hm-2. The changes in grain yield and water and N fertilizer efficiency had no significant difference in treatments of commercial organic fertilizer substitution compared to T1 treatment in wet year. T2 and T3 treatments increased grain yield by 15.6% and 18.2%, and with 35.1% and 27.0% enhancement in harvest index compared to T1. T2 and T3 treatments increased water use efficiency, rainfall use efficiency, N agrono-mic efficiency and partial productivity of N fertilizer by 17.4% and 22.3%, 15.7% and 17.7%, 15.6% and 18.0%, 155.2% and 179.3%. These results demonstrated that under the same N input level, 50% and 37.5% substitution inorganic-N with organic-N could be a suitable substitution rate under plastic film fully mulched ridge-furrow, which could be recommended as a fertilizer application pattern in this area.


Assuntos
Agricultura/métodos , Fertilizantes , Nitrogênio/análise , Zea mays/crescimento & desenvolvimento , Plásticos , Solo , Água
14.
J Forensic Leg Med ; 63: 7-10, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30825772

RESUMO

Acute poisoning is a public health threat that leads to morbidity and mortality worldwide. In this retrospective study, we evaluated autopsies from deaths caused by acute poisoning from 2008 to 2017 at the School of Forensic Medicine, China Medical University (SFMCMU) in the Liaoning Province, northeast China. A total of 140 poisoning deaths were investigated and the demographic characteristics, causes and manner of death, toxics category were analyzed. The number of poisoning deaths gradually increased during the study period. The majority of poisoning deaths were accidents (66.43%), followed by suicides (27.86%), and homicides (3.57%). Of the 140 cases, 47 (33.57%) were caused by drugs, 38 (27.14%) by agrochemicals (mainly organophosphate pesticides, n = 16), 37 (26.43%) by respiratory dysfunction toxics (mainly carbon monoxide, n = 21), and 9 (6.43%) by poisonous plants and animals. Alcohol/methanol (5, 3.57%) and other chemicals (4, 2.86%) accounted for the remaining cases. The present study provides poisoning distribution in the Liaoning province and highlights implications for public health policy and prevention efforts in northeast China.


Assuntos
Envenenamento/mortalidade , Acidentes/mortalidade , Adolescente , Adulto , Distribuição por Idade , Agroquímicos/envenenamento , Concentração Alcoólica no Sangue , Intoxicação por Monóxido de Carbono/mortalidade , Criança , China/epidemiologia , Feminino , Toxicologia Forense , Homicídio/estatística & dados numéricos , Humanos , Masculino , Pessoa de Meia-Idade , Insuficiência de Múltiplos Órgãos/mortalidade , Plantas Tóxicas/toxicidade , Insuficiência Respiratória/mortalidade , Estudos Retrospectivos , Distribuição por Sexo , Suicídio/estatística & dados numéricos , Adulto Jovem
15.
Rev Sci Instrum ; 90(2): 023911, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30831698

RESUMO

We report on the resistance behavior of bare-chip Cernox thermometers under pressures up to 2 GPa, generated in a piston-cylinder pressure cell. Our results clearly show that Cernox thermometers, frequently used in low-temperature experiments due to their high sensitivity, remain highly sensitive even under applied pressure. We show that these thermometers are therefore ideally suited for measurements of heat capacity under pressure utilizing an ac oscillation technique up to at least 150 K. Our Cernox-based system is very accurate in determining changes in the specific heat as a function of pressure as demonstrated by measurements of the heat capacity on three different test cases: (i) the superconducting transition in elemental Pb (Tc = 7.2 K), (ii) the antiferromagnetic transition in the rare-earth compound GdNiGe3 (TN = 26 K), and (iii) the structural/magnetic transition in the iron-pnictide BaFe2As2 (Ts,N = 130 K). The chosen examples demonstrate the versatility of our technique for measuring the specific heat under the pressure of various condensed-matter systems with very different transition temperatures as well as amounts of removed entropy.

16.
Solid State Nucl Magn Reson ; 98: 12-18, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30669006

RESUMO

The sensitivity of solid-state NMR experiments that utilize 1H zero-quantum heteronuclear dipolar recoupling, such as D-HMQC, is compromised by poor homonuclear decoupling. This leads to a rapid decay of recoupled magnetization and an inefficient recoupling of long-range dipolar interactions, especially for nuclides with low gyromagnetic ratios. We investigated the use, in symmetry-based 1H heteronuclear recoupling sequences, of a basic R element that was principally designed for efficient homonuclear decoupling. By shortening the time required to suppress the effects of homonuclear dipolar interactions to the duration of a single inversion pulse, spin diffusion was effectively quenched and long-lived recoupled coherence lifetimes could be obtained. We show, both theoretically and experimentally, that these modified sequences can yield considerable sensitivity improvements over the current state-of-the-art methods and applied them to the indirect detection of 89Y in a metal-organic framework.

17.
Histol Histopathol ; 34(7): 745-753, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30516266

RESUMO

HIPK2 is an evolutionarily conserved serine/threonine kinase and is considered a co-regulator of an increasing number of transcription factors modulating a variety of cellular processes, including inflammation, proliferation and fibrosis. Skeletal muscle injuries repair is an overlapping event between inflammation and tissue repair. There are no reports about HIPK2 expression in skeletal muscles after trauma. A foundational study on distribution and time-dependent expression of HIPK2 was performed by immunohistochemical staining, Western blotting and quantitative real-time PCR, which is expected to obtain a preliminary insight into the functions of HIPK2 during the repair of contused skeletal muscle in mice. An animal model of skeletal muscle contusion was established in 50 C57B6/L male mice. Samples were taken at 1, 3, 5, 7, 9, 14, 17, 21 and 28 days after contusion, respectively (5 mice at each posttraumatic interval). 5 mice were employed as control. No HIPK2-positive staining was detected in uninjured skeletal muscle. Intensive immunoreactivties of HIPK2 were observed in polymorphonuclear cells, round-shaped mononuclear cells, regenerated multinucleated myotubes and spindle-shaped fibroblastic cells in the contused tissue. The HIPK2-positive cells were identified as neutrophils, macrophages and myofibroblasts by double immunofluorescent procedure. HIPK2 protein and mRNA expression were remarkably up-regulated after contusion by Western blotting and qPCR analysis. The results demonstrated that the expression of HIPK2 is distributed in certain cell types and is time-dependently expressed in skeletal muscle after contusion, which suggested that HIPK2 may participate in the whole process of skeletal muscle wound healing, including inflammatory response, muscle regeneration and fibrogenesis.


Assuntos
Contusões/enzimologia , Músculo Esquelético/enzimologia , Músculo Esquelético/lesões , Proteínas Serina-Treonina Quinases/metabolismo , Cicatrização , Animais , Contusões/patologia , Fibroblastos/citologia , Fibroblastos/enzimologia , Fibrose , Inflamação/enzimologia , Inflamação/patologia , Macrófagos/citologia , Macrófagos/enzimologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Fibras Musculares Esqueléticas/enzimologia , Músculo Esquelético/patologia , Miofibroblastos/citologia , Miofibroblastos/enzimologia , Neutrófilos/citologia , Neutrófilos/enzimologia , Regeneração , Fatores de Tempo
18.
Neurosci Lett ; 694: 161-167, 2019 02 16.
Artigo em Inglês | MEDLINE | ID: mdl-30528875

RESUMO

Neuronal death and organization degeneration can happen inordinately after spinal cord injury (SCI), which lead to nerve dysfunction. We aimed to determine whether local application of a cell permeable calpain I inhibitor (MDL28170) can promote SCI recovery by increasing neuronal cell viability. MDL28170-loaded polycaprolactone (PCL) film was fabricated. Scanning electron microscopy showed the surface of PCL film was smooth with holes (diameter at µM level). The PCL film was non-toxic, biological compatibility, and had good neuron adhension and slow release characteristic. MDL28170 increased VSC4.1 motor neurons' viability under tunicamycin (an endoplasmic reticulum stress) induced injury. In a traumatic SCI rat model, MDL28170-loaded PCL film reduced the area of lesion cavity, and promoted recovery of locomotor behavior. Moreover, the expression of GAP-43 was upregulated after MDL28170-loaded PCL film treatment. Thus, our findings demonstrated that localized delivery of MDL28170 could promote SCI recovery by inhibiting endoplasmic reticulum stress, preserving survival of the motor neurons, which may point out a promising therapeutic target for treating SCI patient.


Assuntos
Dipeptídeos/administração & dosagem , Sistemas de Liberação de Medicamentos/métodos , Neurônios Motores/efeitos dos fármacos , Recuperação de Função Fisiológica/efeitos dos fármacos , Traumatismos da Medula Espinal/tratamento farmacológico , Animais , Materiais Biocompatíveis , Morte Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Feminino , Proteína GAP-43/metabolismo , Gliose/prevenção & controle , Glicoproteínas/administração & dosagem , Locomoção/efeitos dos fármacos , Neurônios Motores/metabolismo , Poliésteres/administração & dosagem , Ratos Sprague-Dawley , Traumatismos da Medula Espinal/metabolismo
19.
Artigo em Inglês | MEDLINE | ID: mdl-30423524

RESUMO

This study investigated pharmacokinetics, tissue distribution and excretion of ACT001 in Sprague-Dawley rats. Stability study and metabolism study of ACT001 are conducted. The absolute bioavailability of ACT001 is 50.82%. ACT001 has no accumulation effect and displayed wide tissue distribution. ACT001 can be rapidly distributed to tissues after oral administration and can diffuse through the blood-brain barrier. The total cumulative excretion of ACT001 in feces, urine and bile were found to be 0.05, 3.42 and 0.012%, respectively. UPLC/ESI-QTOF-MS coupled with MetaboLynx XS software was utilized to detect the metabolites of ACT001 in vitro. Five metabolites (M1, M2, M3, M4 and M5) were detected. M2 wasn't discovered in human liver microsome samples and bile samples. M1 and M2 weren't discovered in rat plasma and human plasma. M3, M4 and M5 weren't discovered in bile samples. M5 is an active metabolite named micheliolide (MCL). There is no significant difference in half-life, type of identified metabolites and the amount of each metabolites between using rat plasma and human plasma. Owing to the species differences of hepatomicrosome enzymes, significant differences were shown in half-life, type of identified metabolites and the amount of each metabolites between using rat liver microsome and human liver microsome.


Assuntos
Sesquiterpenos de Guaiano/metabolismo , Sesquiterpenos de Guaiano/farmacocinética , Administração Oral , Animais , Estabilidade de Medicamentos , Limite de Detecção , Modelos Lineares , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Sesquiterpenos de Guaiano/administração & dosagem , Sesquiterpenos de Guaiano/química , Distribuição Tecidual
20.
Artigo em Inglês | MEDLINE | ID: mdl-30523848

RESUMO

We present an experimental study of the superconducting properties of NiBi<sub>3</sub> as a function of pressure by means of resistivity and magnetization measurements and combine our results with DFT calculations of the band structure under pressure. We find a moderate suppression of the critical temperature <i>T</i><sub>c</sub> from ∼ 4.1 K to ∼ 3 K by pressures up to 2 GPa. By taking into account the change of the band structure as a function of pressure, we argue that the decrease in <i>T</i><sub>c</sub> is consistent with conventional, electron-phonon-mediated BCS-type superconductivity.

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