Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 13 de 13
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
2.
Clin Infect Dis ; 2020 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-32198501

RESUMO

BACKGROUND: Emergence of coronavirus disease 2019 (COVID-19) is a major healthcare threat. Current method of detection involves qPCR-based technique, which identifies the viral nucleic acids when present in sufficient quantity. False negative results can be achieved and failure to quarantine the infected patient would be a major setback in containing the viral transmission. We here aim to describe the time kinetics of various antibodies produced against the 2019 novel coronavirus (SARS-CoV-2) and evaluate the potential of antibody testing to diagnose COVID-19. METHODS: The host humoral response against SARS-CoV-2 including IgA, IgM and IgG response were examined by using an ELISA based assay on the recombinant viral nucleocapsid protein. Total 208 plasma samples were collected from 82 confirmed and 58 probable cases (qPCR negative but had typical manifestation). The diagnostic value of IgM was evaluated in this cohort. RESULTS: The median duration of IgM and IgA antibody detection were 5 days (IQR 3-6), while IgG was detected on 14 days (IQR 10-18) after symptom onset, with a positive rate of 85.4%, 92.7% and 77.9% respectively. In confirmed and probable cases, the positive rates of IgM antibodies were 75.6% and 93.1%, respectively. The detection efficiency by IgM ELISA is higher than that of qPCR method after 5.5 days of symptom onset. The positive detection rate is significantly increased (98.6%) when combined IgM ELISA assay with PCR for each patient compare with a single qPCR test (51.9%). CONCLUSIONS: Humoral response to SARS-CoV-2 can aid to the diagnosis of COVID-19, including subclinical cases.

3.
Clin Infect Dis ; 2020 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-32221523

RESUMO

BACKGROUND: Coronavirus disease 2019 (COVID-19) has become a public health emergency. The widely used reverse transcription PCR (RT-PCR) method has limitations for clinical diagnosis and treatment. METHODS: A total of 323 samples from 76 COVID-19 confirmed patients were analyzed by droplet digital PCR (ddPCR) and RT-PCR based two target genes (ORF1ab and N). Nasal swabs, throat swabs, sputum, blood, and urine were collected. Clinical and imaging data were obtained for clinical staging. RESULTS: In 95 samples tested positive by both methods, the cycle threshold (Ct) of RT-PCR was highly correlated with the copy numbed of ddPCR (ORF1ab gene, R2 = 0.83; N gene, R2 = 0.87). 4 (4/161) negative and 41 (41/67) single-gene positive samples tested by RT-PCR were positive according to ddPCR with viral load ranging from 11.1 to 123.2 copies/test. Then the viral load of respiratory samples was compared and the average viral load in sputum (17429 ± 6920 copies/test) was found to be significantly higher than in throat swabs (2552 ± 1965 copies/test, p < 0.001) and nasal swabs (651 ± 501 copies/test, p < 0.001). Furthermore, the viral load in the early and progressive stages were significantly higher than that in the recovery stage (46800 ± 17272 vs 1252 ± 1027, p < 0.001) analyzed by sputum samples. CONCLUSIONS: Quantitative monitoring of viral load in lower respiratory tract samples helps to evaluate disease progression, especially in cases of low viral load.

4.
J Trop Pediatr ; 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32040187

RESUMO

Previous studies have shown that the CareStart™ G6PD Deficiency rapid diagnostic test has high diagnostic accuracy on G6PD deficiency in Africa and Thailand, but not in China. As there are regional differences of G6PD genotype distribution, we are attending to verify the effectiveness of the kit in Chinese population. The study cohort included 247 newborns admitted to our hospital for jaundice. The quantitative detection of G6PD enzyme activity and G6PD gene mutations analysis was used to classify the status of G6PD deficiency. The performance of CareStart™ assays was verified by calculating the sensitivity, specificity and area under the receiver operating characteristic curve (AUC) based on the corrected G6PD deficiency status. In male newborns, the sensitivity of the CareStart™ assay was 98.9%, the specificity was 94.2% and the AUC was 0.97. In female newborns, the sensitivity was 58.5% when the cutoff value of residual enzyme activity was 100%; however, the sensitivity was 100% when the cutoff value was 60%. Therefore, the CareStart™ test can effectively screen G6PD deficiency in male newborns and female infants with less than 60% residual enzyme activity, female infants with residual enzyme activities of 60-100% are more likely to be missed diagnosed among Chinese newborns.

5.
Int J STD AIDS ; 30(6): 550-556, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30722749

RESUMO

Although infectious diarrhea is one of the most common complications in human immunodeficiency virus (HIV)-infected patients, robust diagnostic methods for determining potential pathogens are still limited in the clinic. Norovirus, a type of calicivirus, has been shown to be the most common cause of gastroenteritis. Here, we used multiplex polymerase chain reaction as a diagnostic tool to verify Norovirus as the diarrhea-related pathogen in HIV-infected patients with unknown etiological information. Stool specimens obtained from 81 HIV-infected patients with diarrhea were analyzed by BioFire FilmArray Gastrointestinal (GI) panel. Among 26 HIV-infected patients with unknown etiological information, we detected Norovirus in 14 stool specimens of these patients with 100% sensitivity and specificity as confirmed by reverse transcription polymerase chain reaction (RT-PCR), and one specimen showed both Norovirus and enterotoxigenic Escherichia coli infection. Among the remaining 55 patients with verified Clostridium difficile infection, nine patients also detected positive for Norovirus infection. In conclusion, using FilmArray GI panel and RT-PCR, we determined that Norovirus infection as one of the main pathogens responsible for diarrhea in HIV patients.


Assuntos
Infecções por Caliciviridae/diagnóstico , Diarreia/diagnóstico , Diarreia/virologia , Fezes/virologia , Gastroenterite/diagnóstico , Infecções por HIV/complicações , Reação em Cadeia da Polimerase Multiplex/métodos , Norovirus/isolamento & purificação , RNA Viral/metabolismo , Adulto , Idoso , Diarreia/microbiologia , Fezes/microbiologia , Feminino , Gastroenterite/virologia , Infecções por HIV/virologia , Humanos , Masculino , Pessoa de Meia-Idade , Norovirus/genética , Norovirus/patogenicidade , RNA Viral/genética , Sensibilidade e Especificidade
6.
J Infect Dev Ctries ; 13(8): 714-719, 2019 08 31.
Artigo em Inglês | MEDLINE | ID: mdl-32069255

RESUMO

INTRODUCTION: Soluble CD163 (sCD163) and soluble CD14 (sCD14) levels, monocyte/macrophage activation markers, are elevated in patient serum during Brucella infection. The aim of this study was to measure serum sCD163 and sCD14 levels during treatment for acute brucellosis to determine whether they can be used to monitor treatment efficacy. METHODOLOGY: Blood samples were collected from 30 patients with acute brucellosis (disease duration < 8 weeks) before and after 6 weeks of antibiotic therapy as well as from a comparison group of 28 healthy control individuals. Serum sCD163 and sCD14 levels were measured with specific, sandwich enzyme-linked immunosorbent assays. The clinical data and routine indices including C-reactive protein (CRP), erythrocyte sedimentation rates (ESR), as well as white cell counts (WBC) were also studied. RESULTS: Both serum sCD163 and sCD14 levels were significantly higher in patients with acute brucellosis than in healthy controls (p < 0.0001). A significant decline was observed in patients after cessation of treatment (p < 0.001), which still be significantly higher than that in healthy controls (p < 0.001). In additional, serum sCD163 levels were positively correlated with sCD14 levels; both of which were positively associated with CRP levels. However, neither sCD163 nor sCD14 levels were correlated with ESR or WBC. CONCLUSIONS: The decline in sCD163 and sCD14 levels following antibiotic therapy may be used as a marker to assess therapeutic efficacy following treatment of acute brucellosis.

7.
PLoS One ; 13(4): e0195452, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29664957

RESUMO

BACKGROUND: The HIV pandemic remains the most serious challenge to public health worldwide. The hallmark characteristics of the disease is the eventual failure of the immune system to control opportunistic infections and death. However not everyone who has HIV develops the disease at the same rate and so we are studying how the immune system works to control the virus in those who have been infected for decades and remain relatively healthy without the need of anti-retroviral therapy (ART). METHODS: Genomic DNA samples from 513 Chinese Han individuals from Henan province were typed for 15 KIR and 3 HLA class I genes. Genotype frequencies were compared between a village cohort of 261 former plasma donors (SM cohort) infected with HIV-1 through an illegal plasma donor scheme who survived more than 10 years of infection without ART and 252 ethnically-matched healthy controls from a nearby village. KIR and HLA were molecularly typed using a combination of polymerase chain reaction (PCR) with sequence-specific primers (PCR-SSP) and sequence based techniques. RESULTS: All 15 KIR genes were observed in the study population at various frequencies. KIR2DL3 was significantly less common in the HIV-1 infected group (95.8% vs 99.2%, p = 0.021). The combination of KIR3DS1 with homozygosity for HLA-Bw4 alleles (the putative ligand for KIR3DS1) was significantly less frequent in the HIV-1 infected group than in the control group (6.0% vs 12.0% respectively, p = 0.023). CONCLUSION: Specific KIR-HLA compound genotypes associate with differential outcomes to infection and disease progression following exposure to a narrow-source HIV-1.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Infecções por HIV/genética , HIV-1/genética , Antígenos HLA-B/genética , Antígenos de Histocompatibilidade Classe I/genética , Receptores KIR3DS1/genética , Receptores KIR/genética , Adulto , Estudos de Casos e Controles , Estudos de Coortes , Progressão da Doença , Feminino , Infecções por HIV/sangue , Infecções por HIV/virologia , Humanos , Masculino , Pessoa de Meia-Idade
8.
Infect Genet Evol ; 45: 83-89, 2016 11.
Artigo em Inglês | MEDLINE | ID: mdl-27566335

RESUMO

Currently, it is still controversial that if the pathogenicity of EV-A71 causing severe or mild hand, foot, and mouth disease (HFMD) is associated with viral nucleotide or amino acid sequence(s). In this study, 19 clinical strains were detected in samples from diagnosed patients of EV-A71-caused HFMD with mild or severe symptoms. Then, VP1-2A fragment sequences of 19 EV-A71 isolates were determined, the phylogenetic analysis, based on VP1 sequences of 19 EV-A71 stains in this study and which of 62 EV-A71 strains with different clinical phenotypes reported before, were carried out. Our results showed that no difference in the genotype and evolution distribution was observed among the EV-A71 strains mentioned above. Furthermore, two EV-A71 isolates, which with much close evolutionary relationship but different clinical manifestations, were purified by plaque assay, the complete genome sequencing was done, and deduced amino acid sequence analysis of 11 proteins coded by EV-A71 was carried out. Eight variable amino acid sites were found and further verified with those of 62 strains reported before. Our study provides further evidence that the potential pathogenicity of EV-A71 causing severe or mild HFMD seems not to be associated with viral genotype and even the amino acid substitution.


Assuntos
Enterovirus/genética , Doença de Mão, Pé e Boca/virologia , Aminoácidos , Proteínas do Capsídeo/genética , Estudos de Coortes , Enterovirus/classificação , Genoma Viral/genética , Doença de Mão, Pé e Boca/diagnóstico , Doença de Mão, Pé e Boca/fisiopatologia , Humanos , Fenótipo , Filogenia , RNA Viral/análise , RNA Viral/genética , Análise de Sequência de RNA
9.
Artigo em Inglês | MEDLINE | ID: mdl-25585387

RESUMO

Perovskite ferroelectrics (PFs) have been the dominant piezoelectric materials for various electromechanical applications, such as ultrasonic transducers, sensors, and actuators, to name a few. In this review article, the development of PF crystals is introduced, focusing on the crystal growth and piezoelectric activity. The critical factors responsible for the high piezoelectric activity of PFs (i.e., phase transition, monoclinic phase, domain size, relaxor component, dopants, and piezoelectric anisotropy) are surveyed and discussed. A general picture of the present understanding on the high piezoelectricity of PFs is described. At the end of this review, potential approaches to further improve the piezoelectricity of PFs are proposed.

10.
PLoS One ; 8(6): e67430, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23840697

RESUMO

BACKGROUND: Enterovirus 71 (EV71) infection can lead to a rapidly progressing, life-threatening, and severe neurological disease in young children, including the development of human hand, foot, and mouth disease (HFMD). This study aims to further characterize the specific immunological features in EV71-mediated HFMD patients presenting with differing degrees of disease severity. METHODOLOGY: Comprehensive cytokine and chemokine expression were broadly evaluated by cytokine antibody array in EV71-infected patients hospitalized for HFMD compared to Coxsackievirus A16-infected patients and age-matched healthy controls. More detailed analysis using Luminex-based cytokine bead array was performed in EV71-infected patients stratified into diverse clinic outcomes. Additionally, immune cell frequencies in peripheral blood and EV71-specific antibodies in plasma were also examined. PRINCIPAL FINDINGS: Expression of several cytokines and chemokines were significantly increased in plasma from EV71-infected patients compared to healthy controls, which further indicated that: (1) GM-CSF, MIP-1ß, IL-2, IL-33, and IL-23 secretion was elevated in patients who rapidly developed disease and presented with uncomplicated neurological damage; (2) G-CSF and MCP-1 were distinguishably secreted in EV71 infected very severe patients presenting with acute respiratory failure; (3) IP-10, MCP-1, IL-6, IL-8, and G-CSF levels were much higher in cerebrospinal fluid than in plasma from patients with neurological damage; (4) FACS analysis revealed that the frequency of CD19(+)HLADR(+) mature B cells dynamically changed over time during the course of hospitalization and was accompanied by dramatically increased EV71-specific antibodies. Our data provide a panoramic view of specific immune mediator and cellular immune responses of HFMD and may provide useful immunological profiles for monitoring the progress of EV71-induced fatal neurological symptoms with acute respiratory failure.


Assuntos
Quimiocinas/sangue , Enterovirus Humano A/imunologia , Doença de Mão, Pé e Boca/imunologia , Anticorpos Antivirais/sangue , Quimiocinas/líquido cefalorraquidiano , Pré-Escolar , Enterovirus/imunologia , Feminino , Doença de Mão, Pé e Boca/sangue , Doença de Mão, Pé e Boca/patologia , Doença de Mão, Pé e Boca/virologia , Humanos , Imunidade Celular , Fatores Imunológicos/sangue , Fatores Imunológicos/líquido cefalorraquidiano , Lactente , Masculino , Doenças do Sistema Nervoso/sangue , Doenças do Sistema Nervoso/imunologia , Doenças do Sistema Nervoso/patologia , Doenças do Sistema Nervoso/virologia , Análise Serial de Proteínas , Índice de Gravidade de Doença , Carga Viral
11.
Zhonghua Jie He He Hu Xi Za Zhi ; 33(10): 727-9, 2010 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-21176500

RESUMO

OBJECTIVE: To evaluate the clinical value of bronchoscopy in the pathogenic diagnosis of AIDS patients with pulmonary infections and to illustrate the constituent ratio of different pulmonary pathogens. METHODS: From August 2006 to September 2009, we performed bronchoscopies to 120 AIDS patients who had pulmonary infections. We described the manifestations under the bronchoscope and each patient underwent bronchoalveolar lavage for further detection including bacterial culture and pathological test. We also took biopsies in patients who had obviously abnormal lesions under the bronchoscope.At the same time, we collected the clinical information for analyzing. RESULTS: Among 120 patients, we found 30 cases of mycobacteria infection, 25 cases of bacterial infection, 12 cases of PCP, 5 cases of fungal positive, 3 cases of CMV. Bronchial mucosa biopsies were taken in 26 patients, 12 cases of chronic inflammation, 7 cases of granulomatous inflammation, 4 cases of squamous cell carcinoma, 2 cases of adenocarcinoma and 1 case of lymphoma. CONCLUSION: Bronchoscopy is a very useful tool and it's of great value for pathogenic detection in AIDS patients with pulmonary infections. At present, in China the main pulmonary infections in AIDS patients are TB, bacterial infection and PCP.


Assuntos
Síndrome de Imunodeficiência Adquirida/microbiologia , Broncoscopia , Pneumopatias/diagnóstico , Neoplasias Pulmonares/diagnóstico , Infecções por Mycobacterium/diagnóstico , Síndrome de Imunodeficiência Adquirida/patologia , Adulto , Feminino , Humanos , Pneumopatias/microbiologia , Pneumopatias/patologia , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Infecções por Mycobacterium/patologia , Adulto Jovem
12.
Liver Int ; 23 Suppl 3: 16-20, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-12950956

RESUMO

BACKGROUND: Molecular Adsorbents Recirculating System (MARS) is a new promising artificial liver support therapy, the aim of this study was to assess the effectiveness of MARS to remove nitrous oxide (NO) and cytokines in severe liver failure patients with multiple organ dysfunction syndrome (MODS). METHODS: Sixty single MARS treatments were performed with length of 6-24 h on 24 severe liver failure patients (18 males/6 females) with MODS. RESULTS: The MARS therapy was associated with a significant removal of NO and certain cytokines such as TNF-alpha, IL-6, IL-8, and INF-gamma, together with marked reduction of other non-water-soluble albumin bound toxins and water-soluble toxins, these were associated with a improvement of the patients' clinical conditions including hepatic encephalopathy, deranged hemodynamic situation and as well as renal and respiratory function, thus resulted into marked decrease of Sequential Organ Failure Assessment (SOFA) score and improved outcome: nine patients were able to be discharged from the hospital or bridged to successful liver transplantation, the overall survival of 24 patients was 37.5%. CONCLUSION: We can confirm the positive therapeutic impact and safety to use MARS on liver failure patients with MODS associated with elevated levels of NO and cytokines.


Assuntos
Citocinas/sangue , Falência Hepática Aguda/terapia , Insuficiência de Múltiplos Órgãos/terapia , Óxido Nitroso/sangue , Diálise Renal , Desintoxicação por Sorção , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Interferon gama/sangue , Interleucina-6/sangue , Interleucina-8/sangue , Falência Hepática Aguda/sangue , Masculino , Pessoa de Meia-Idade , Insuficiência de Múltiplos Órgãos/sangue , Índice de Gravidade de Doença , Toxinas Biológicas/sangue , Fator de Necrose Tumoral alfa/metabolismo
13.
Zhonghua Gan Zang Bing Za Zhi ; 11(8): 455-7, 2003 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-12939172

RESUMO

OBJECTIVE: To evaluate the effectiveness and mechanisms of molecular adsorbents recirculating system (MARS) treatment in severe liver failure patients with multiple organ dysfunction syndrome (MODS). METHODS: 60 single MARS treatments were performed for 6 - 24 hours on 24 severe liver failure patients with MODS. RESULTS: MARS therapy was associated with marked reduction of albumin bound toxins and water soluble toxins, together with a significant removal of NO and certain cytokines, such as TNF-alpha, IL-6, IL-8, and INF-gamma. These were associated with a improvement of the patients' clinical conditions including hepatic encephalopathy, deranged hemodynamic situation, as well as renal and respiratory function, thus resulted into marked decrease of sequential organ failure assessment (SOFA) score (from 9.72+-1.89 to 6.98+-2.34), and improving outcome: 9 patients were able to be discharged from the hospital or bridged to successful liver transplantation. The overall survival rate of 24 patients was 37.5%. CONCLUSIONS: There is positive therapeutic impact and safety to use MARS on liver failure patients with MODS. The effectiveness of MARS is correlated with reducing the levels of NO and cytokines, except for completely removing of accumulated toxins in liver failure patients.


Assuntos
Falência Hepática Aguda/terapia , Fígado Artificial , Insuficiência de Múltiplos Órgãos/terapia , Desintoxicação por Sorção , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Reatores Biológicos , Feminino , Humanos , Interferon gama/sangue , Interleucina-6/sangue , Interleucina-8/sangue , Falência Hepática Aguda/sangue , Masculino , Pessoa de Meia-Idade , Óxido Nítrico/sangue , Desintoxicação por Sorção/instrumentação , Desintoxicação por Sorção/métodos , Fator de Necrose Tumoral alfa/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA