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1.
J Cell Sci ; 135(5)2022 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-33975358

RESUMO

Lipid droplets (LDs) are lipid-rich organelles universally found in most cells. They serve as a key energy reservoir, actively participate in signal transduction and dynamically communicate with other organelles. LD dysfunction has been associated with a variety of diseases. The content level, composition and mobility of LDs are crucial for their physiological and pathological functions, and these different parameters of LDs are subject to regulation by genetic factors and environmental inputs. Coherent Raman scattering (CRS) microscopy utilizes optical nonlinear processes to probe the intrinsic chemical bond vibration, offering label-free, quantitative imaging of lipids in vivo with high chemical specificity and spatiotemporal resolution. In this Review, we provide an overview over the principle of CRS microscopy and its application in tracking different parameters of LDs in live cells and organisms. We also discuss the use of CRS microscopy in genetic screens to discover lipid regulatory mechanisms and in understanding disease-related lipid pathology.


Assuntos
Microscopia , Análise Espectral Raman , Biologia , Gotículas Lipídicas , Lipídeos
2.
IEEE Trans Med Imaging ; PP2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34520349

RESUMO

Drusen is considered as the landmark for diagnosis of AMD and important risk factor for the development of AMD. Therefore, accurate segmentation of drusen in retinal OCT images is crucial for early diagnosis of AMD. However, drusen segmentation in retinal OCT images is still very challenging due to the large variations in size and shape of drusen, blurred boundaries, and speckle noise interference. Moreover, the lack of OCT dataset with pixel-level annotation is also a vital factor hindering the improvement of drusen segmentation accuracy. To solve these problems, a novel multi-scale transformer global attention network (MsTGANet) is proposed for drusen segmentation in retinal OCT images. In MsTGANet, which is based on U-Shape architecture, a novel multi-scale transformer non-local (MsTNL) module is designed and inserted into the top of encoder path, aiming at capturing multi-scale non-local features with long-range dependencies from different layers of encoder. Meanwhile, a novel multi-semantic global channel and spatial joint attention module (MsGCS) between encoder and decoder is proposed to guide the model to fuse different semantic features, thereby improving the model's ability to learn multi-semantic global contextual information. Furthermore, to alleviate the shortage of labeled data, we propose a novel semi-supervised version of MsTGANet (Semi-MsTGANet) based on pseudo-labeled data augmentation strategy, which can leverage a large amount of unlabeled data to further improve the segmentation performance. Finally, comprehensive experiments are conducted to evaluate the performance of the proposed MsTGANet and Semi-MsTGANet. The experimental results show that our proposed methods achieve better segmentation accuracy than other state-of-the-art CNN-based methods.

3.
Brain Behav ; : e2347, 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34520631

RESUMO

BACKGROUND: Fine operation has been an eternal topic in neurosurgery. There were many problems in functional neurosurgery field with high precision requirements. Our study aims to explore the operability, accuracy and postoperative effect of robot-assisted stereoelectroencephalography (SEEG) in neurosurgery. METHODS: We conducted a retrospective analysis of patients with epilepsy who underwent electrode implantation in our hospital. From 2016 to 2019, the epilepsy center of Hebei people's hospital implanted electrodes in neurosurgery on 24 patients, including 20 with SINO robot-assisted SEEG system and eight with frame-SEEG technology. RESULT: Robot-assisted SEEG neurosurgery had higher accuracy, and the mean error of entry and target point was smaller than that of frame SEEG surgery. No bleeding or infection occurred postoperatively, and two patients who underwent robot-assisted SEEG neurosurgery had electrode displacement. Electrode displacement was observed in two patients, both the entry points were orbital frontal, one in the frame system and one in the robot assistant system. The average placement time of each electrode in robot assisted system surgery was less than that in frame system surgery. CONCLUSION: The SINO SEEG electrode implantation assisted by surgical robot-assistant system manufactured in China is safe, accurate and mature.

4.
Artigo em Inglês | MEDLINE | ID: mdl-34521263

RESUMO

Fluorescent probes and mass spectrometry are the two most popular and complementary methods to quantify thiols in biological systems. In this review, we focus on the widely used and commercially available methods to detect and quantify thiols in living cells and the general approaches applied in mass spectrometry-based thiol quantification. We hope this review can serve as a general guide for redox biologists who are interested in thiol species.

5.
Artigo em Inglês | MEDLINE | ID: mdl-34523667

RESUMO

Previous studies reported that Agrimonia pilosa (AP) Ledeb possessed diverse biological activities, including anti-inflammatory, antioxidant, and anti-tumor activities. However, the effect of AP on ulcerative colitis (UC) remains unclear. In this study, we investigated the therapeutic effect and mechanisms of AP on dextran sodium sulfate (DSS)-induced colitis. The potential constituents of AP were investigated by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). A total of 13 compounds were recognized by UPLC-Q-TOF/MS chromatogram. Furthermore, a network pharmacology approach revealed that there are 297 candidate targets of UC and 549 common targets for the 13 active ingredients of AP. GO enrichment and KEGG pathway analysis indicated that AP might have a protective effect on UC through the nuclear factor κB (NF-κB) and nucleotide-binding oligomerization domain (NOD)-like receptor signaling pathways. Subsequent experimental validation in a DSS-induced colitis model revealed that AP alleviated the severity of DSS-induced colitis, reduced the production of proinflammatory factors, and protected against the loss of intestinal integrity. Moreover, AP inhibited the phosphorylation of NF-κB p65 and the activation of the NLRP3 inflammasome. In conclusion, AP ameliorated DSS-induced colitis through suppressing the activation of the NLRP3 inflammasome and NF-κB signaling pathways.

7.
Int J Biol Sci ; 17(13): 3441-3455, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34512158

RESUMO

Crotonylation is a kind of newly discovered acylation modification. Thousands of crotonylation sites have been identified in histone and non-histone proteins over the past decade. As a modification closely related to acetylation, crotonylation was reported to share many universal enzymes with acetylation. Crotonylated proteins have important roles in the regulation of various biological processes, such as gene expression, process of spermatogenesis, cell cycle, and also in the pathogenesis of different diseases, which range from depression to cancer. In this review, we summarize the research processes of crotonylation and discuss the advances of regulation mechanism of both histone and non-histone proteins crotonylation in difference physiological processes. Also, we focus on the alteration of the crotonylation under certain pathological conditions and its role in the pathogenesis of each disease.

8.
Langmuir ; 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34519205

RESUMO

It is a great challenge to precisely control the molecules that self-assemble into diverse shapes with diverse properties. Herein, the self-assembled behaviors between calixpyridinium and two pyrenesulfonate guests, 1,3,6,8-pyrenetetrasulfonic acid tetrasodium salt (PyTS) and sodium 1-pyrenesulfonate (PS), were studied. The morphology and property of the two assemblies were quite different. PS guests self-assembled into spherical aggregates upon complexation with calixpyridinium, while the self-assembled rodlike aggregates were formed via the binding between calixpyridinium and PyTS guests. The calixpyridinium-PS supramolecular aggregates could not emit fluorescence in the solid state, while a strong green fluorescence was emitted by the calixpyridinium-PyTS supramolecular aggregates in the solid state. More interestingly and importantly, the solid calixpyridinium-PyTS supramolecular aggregates exhibited an adsorbent ability to iodine in both the aqueous solution and the vapor phase, while the solid calixpyridinium-PS supramolecular aggregates could not capture iodine. The diverse iodine capture capability of the two supramolecular aggregates was determined by the self-assembled structure at the molecular level.

9.
Hum Brain Mapp ; 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34520078

RESUMO

Exploring typical and atypical brain developmental trajectories is very important for understanding the normal pace of brain development and the mechanisms by which mental disorders deviate from normal development. A precise and sex-specific brain age prediction model is desirable for investigating the systematic deviation and individual heterogeneity of disorders associated with atypical brain development, such as autism spectrum disorders. In this study, we used partial least squares regression and the stacking algorithm to establish a sex-specific brain age prediction model based on T1-weighted structural magnetic resonance imaging and resting-state functional magnetic resonance imaging. The model showed good generalization and high robustness on four independent datasets with different ethnic information and age ranges. A predictor weights analysis showed the differences and similarities in changes in structure and function during brain development. At the group level, the brain age gap estimation for autistic patients was significantly smaller than that for healthy controls in both the ABIDE dataset and the healthy brain network dataset, which suggested that autistic patients as a whole exhibited the characteristics of delayed development. However, within the ABIDE dataset, the premature development group had significantly higher Autism Diagnostic Observation Schedule (ADOS) scores than those of the delayed development group, implying that individuals with premature development had greater severity. Using these findings, we built an accurate typical brain development trajectory and developed a method of atypical trajectory analysis that considers sex differences and individual heterogeneity. This strategy may provide valuable clues for understanding the relationship between brain development and mental disorders.

10.
Int J Mol Sci ; 22(17)2021 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-34502218

RESUMO

Phosphorus (P) deficiency is an important challenge the world faces while having to increase crop yields. It is therefore necessary to select maize (Zea may L.) genotypes with high phosphorus use efficiency (PUE). Here, we extensively analyzed the biomass, grain yield, and PUE-related traits of 359 maize inbred lines grown under both low-P and normal-P conditions. A significant decrease in grain yield per plant and biomass, an increase in PUE under low-P condition, as well as significant correlations between the two treatments were observed. In a genome-wide association study, 49, 53, and 48 candidate genes were identified for eleven traits under low-P, normal-P conditions, and in low-P tolerance index (phenotype under low-P divided by phenotype under normal-P condition) datasets, respectively. Several gene ontology pathways were enriched for the genes identified under low-P condition. In addition, seven key genes related to phosphate transporter or stress response were molecularly characterized. Further analyses uncovered the favorable haplotype for several core genes, which is less prevalent in modern lines but often enriched in a specific subpopulation. Collectively, our research provides progress in the genetic dissection and molecular characterization of PUE in maize.

11.
Sci Total Environ ; 802: 149891, 2021 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-34474296

RESUMO

Animals exposure to polychlorinated biphenyls (PCBs) may result in retention of hydroxylated PCBs (OH-PCBs). OH-PCBs can be accumulated in animals, including humans, through the transmission of food chain. However, there are few studies on the accumulation and metabolism of OH-PCBs exposed to the body through daily diet. Therefore, this study was conducted to investigate the fate of OH-PCBs after being ingested through dietary intake. By adding 3-OH-PCB101 and 4-OH-PCB101 to the edible tissue of crucian carp, which were used as raw materials to prepare mouse feed, with an exposure concentration of 2.5 µg/kg ww. The exposure experiment lasted for a total of 80 days. The blood, feces and 11 tissues of mice at different times were analyzed qualitatively and quantitatively. It was found that major OH-PCB101 were accumulated in intestine or excreted with feces. A small part was accumulated in heart, lung and spleen. For the first time that the conversion from OH-PCB101 to PCB101 in mice was discovered, which shows from another perspective that persistent organic pollutants are difficult to be completely degraded in the environment. 4-MeO-PCB101, 3-MeSO2-PCB101, and 4-MeSO2-PCB101 were also found in various tissues. The results of this study show that after OH-PCBs accumulated in animals re-enter the organism through the food chain, they can be metabolized again and may be reversely transformed into the parent compounds. The present research shed new light on simulating the metabolic transformation process of OH-PCBs exposed to mammals through ingestion of fish. Available data show that second-generation persistent organic pollutants in the environment still need to be continuously concerned.

12.
Stem Cells ; 2021 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-34486791

RESUMO

Aberrant lipid metabolism has recently been recognized as a new hallmark of malignancy, but the characteristics of fatty acid metabolism in breast cancer stem cells (BCSC) and potential interventions targeting this pathway remain to be addressed. Here, by using the in vitro BCSC models, mammosphere-derived MCF-7 cells and HMLE-Twist-ER cells, we found that the cells with stem cell-like properties exhibited a very distinct profile of fatty acid metabolism compared with that of their parental cancer cells, characterized by increased lipogenesis, especially the activity of stearoyl-CoA desaturase 1 (SCD1) responsible for the production of monounsaturated fatty acids (MUFA), and augmented synthesis and utilization of the omega-6 arachidonic acid (AA). Suppression of SCD1 activity by either enzyme inhibitors or siRNA knockdown strikingly limited self-renewal and growth of the BCSC, suggesting a key role for SCD1 in BCSC proliferation. Furthermore, elevated levels of SCD1 and other lipogenic enzymes were observed in human breast cancer tissues relative to the non-cancer tissues from the same patients and correlated with the pathological grades. Interestingly, treatment of BCSC with omega-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), effectively down-regulated the expression of the lipogenic enzymes and markedly suppressed BCSC self-renewal and growth. Dietary supplementation of nude mice bearing BCSC-derived tumors with omega-3 fatty acids also significantly reduced their tumor load. These findings have demonstrated that increased lipogenesis is critical for self-renewal and growth of breast cancer stem cells, and that omega-3 fatty acids are effective in targeting this pathway to exert their anti-cancer effect. © AlphaMed Press 2021 SIGNIFICANCE STATEMENT: Dysregulated lipid metabolism is proved to be associated with the growth of cancer stem cells (CSC); however, the potential interventions targeting this pathway remains to be elucidated. Our study has demonstrated that breast CSC have a distinct fatty acid profile due to the aberrant expressions of lipogenic enzymes, which are highly involved in sustaining their self-renewal, and proliferative capabilities, and that omega-3 PUFA can effectively suppress the self-renewal and growth of breast CSC by downregulation of the lipogenic enzymes, especially SCD1. These findings provide a potential, the rapeutic application of omega-3 PUFA by targeting fatty acid metabolism in breast CSC.

13.
Environ Monit Assess ; 193(9): 614, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34468858

RESUMO

Habitat quality is an important indicator for measuring regional biodiversity and ecosystem service value. A change in habitat quality is the direct result of the interaction between human activities and the natural environment. In this study, the Integrated Valuation of Ecosystem Services and Trade-offs (InVEST) model was used to evaluate the habitat quality of the Yellow River Basin (YRB) from 1980 to 2018. We further analyzed the quantity and spatial transfer status of habitat quality quantitatively using the Geo-informatic Tupu method. The results show that the habitat degradation degree under human disturbance showed a trend of increasing first and then decreasing, with values of 0.0196 in 1980, 0.0200 in 2000, and 0.0199 in 2018. In addition, it presents two ring structures: light-severe-high-moderate and light-moderate-high-severe in space. The overall level of habitat quality in the basin is relatively good, but there is a trend of decline, which are 0.6091, 0.6069, and 0.6049 in the three stages respectively. The spatial distribution of habitat quality showed a pattern of high in the middle and low on both sides. The habitat quality has been restored in some areas. The transition between good and medium and good and excellent in the Tupu change units of the habitat quality grade is the most intense. Both stages are mainly the transformation from high-grade to low-grade habitat quality, but there is a trend of gradual improvement. The findings could have theoretical support and policy implications for the maintenance of biodiversity and the protection of the natural environment in the Yellow River Basin.


Assuntos
Ecossistema , Rios , China , Conservação dos Recursos Naturais , Monitoramento Ambiental , Humanos
14.
Bioinformatics ; 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34478501

RESUMO

MOTIVATION: Precise prediction of cancer subtypes is of significant importance in cancer diagnosis and treatment. Disease etiology is complicated existing at different omics levels, hence integrative analysis provides a very effective way to improve our understanding of cancer. RESULTS: We propose a novel computational framework, named Deep Subspace Mutual Learning (DSML). DSML has the capability to simultaneously learn the subspace structures in each available omics data and in overall multi-omics data by adopting deep neural networks, which thereby facilitates the subtypes prediction via clustering on multi-level, single level, and partial level omics data. Extensive experiments are performed in five different cancers on three levels of omics data from The Cancer Genome Atlas. The experimental analysis demonstrates that DSML delivers comparable or even better results than many state-of-the-art integrative methods. AVAILABILITY: An implementation and documentation of the DSML is publicly available at https://github.com/polytechnicXTT/Deep-Subspace-Mutual-Learning.git. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

15.
Front Endocrinol (Lausanne) ; 12: 689079, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34484113

RESUMO

Background: Epidemiological trends of type 2 diabetes mellitus attributable to fine particulate matter (PM2.5) pollution remain unclear. Here, we estimated spatiotemporal trends of type 2 diabetes mellitus burden attributable to PM2.5 pollution, including ambient particulate matter pollution (APMP) and household air pollution (HAP), from 1990-2019. Methods: Data were obtained from the Global Burden of Disease Study 2019 and were analyzed by age, sex, year, and location. Joinpoint regression analysis was applied in the analysis of temporal trends in type 2 diabetes mellitus burden over the 30 years. Results: Globally, PM2.5 pollution contributed to 292.5 thousand deaths and 13 million disability-adjusted life-years (DALYs) in 2019. APMP ranked third among all risk factors, causing an increase in type 2 diabetes mellitus burden from 1990, whereas the impact of HAP significantly fell during the same period. Both APMP and HAP contributed the most to deaths and DALYs of type 2 diabetes mellitus among older people. However, the age-standardized death and DALY rates of type 2 diabetes mellitus attributable to APMP were greater among males and people in the middle socio-demographic index countries, especially in Southern Sub-Saharan Africa. For HAP, type 2 diabetes mellitus burden was modestly higher in females and was highest in Oceania, which was the only region with an increase from 1990. Conclusions: PM2.5 pollution resulted in substantial and increasing type 2 diabetes mellitus burden worldwide. Hence, governments and health systems should take steps to reduce air pollution to mitigate this increasing burden.

16.
Zhongguo Zhong Yao Za Zhi ; 46(15): 3846-3852, 2021 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-34472258

RESUMO

The lignans in Urtica cannabina were isolated by preparative HPLC, silica, and ODS column chromatographies, and identified by NMR and HR-MS. The inhibitory activities on 5α-reductase were evaluated in vitro. As a result, ten secolignans,(2R,4S)-2,4-bis(3-methoxyl-4-hydroxyphenyl)-3-butoxypropanol(1), 3,4-trans-3-hydroxymethyl-4-[bis(3,4-dimethoxyphenyl)methyl] butyrolactone(2), 3,4-trans-3-hydroxymethyl-4-[(3,4-dimethoxyphenyl)(3-methoxyl-4-hydroxyphenyl)methyl] butyrolactone(3), 3,4-trans-3-hydroxymethyl-4-[bis(3-methoxyl-4-hydroxyphenyl)methyl] butyrolactone(trans urticol, 4), 3,4-trans-3-hydroxymethyl-4-[bis(3,4-dimethoxyphenyl)methyl] butyrolactone-3-O-ß-D-glucopyranoside(5), 3,4-trans-3-hydroxymethyl-4-[(3,4-dimethoxyphenyl)(3-methoxyl-4-hydroxyphenyl)methyl]butyrolactone-3-O-ß-D-glucopyranoside(6), 3,4-trans-3-hydroxymethyl-4-[bis(3-methoxyl-4-hydroxyphenyl)methyl]butyrolactone-3-O-ß-D-glucopyranoside(trans-urticol-7-O-ß-D-glucopyranoside, 7), cycloolivil-4-O-ß-D-glucopyranoside(8), isolariciresinol-4'-O-ß-D-glucopyranoside(9), and olivil-4'-O-ß-D-glucopyranoside(10), together with a polyphenol [α-viniferin(11)], were isolated from U. cannabina for the first time. Compound 1 was a new lignan. Compound 7 was potent in inhibiting 5α-reductase.


Assuntos
Colestenona 5 alfa-Redutase/farmacologia , Lignanas , Urticaceae , Inibidores de 5-alfa Redutase , Cromatografia Líquida de Alta Pressão , Lignanas/farmacologia , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Urticaceae/efeitos dos fármacos , Urticaceae/enzimologia
17.
J Hazard Mater ; 416: 126079, 2021 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-34492898

RESUMO

Periodic flooding in paddy soils impacts redox behavior and induces variations in pe+pH levels. Manganese (Mn) is capable of reducing cadmium (Cd) uptake by rice. However, the processes involved in how Mn alters Cd mobilization under different pe+pH environments remain poorly understood. To investigate the mechanisms of Mn-mediated soil Cd-stabilization and subsequent inhibition of Cd uptake from flooded soils, we examined Cd immobilization in soil pot incubations, transcriptional changes in Cd-transport genes, and metabolomic analyses of roots and rhizosphere soils with or without Mn application. We found a decrease in extractable Cd concentration largely depended on irrigation-associated low pe+pH, exogenous Mn enhancement of Fe-Mn (oxyhydro)oxide-mediated Cd transformation, and Cd deposition in rice Fe/Mn plaques. Mn application led to striking effects on the expression of Cd-related genes eg. IRT, HMA, and NRAMP in rice root tissue. Exposure to Mn under variable pe+pH levels resulted in metabolic reprogramming of soil and rice roots. Mn induced amino acid synthesis in rice roots, leading to rhizosphere accumulation of free L-lysine, glycine, and glutamine, which can reportedly bind metal ions, forming complexes with Cd. Thus, secreted amino acids, low pe+pH, and free Mn can together comprise a multi-faceted approach to managing Cd toxicity in rice.

18.
Medicine (Baltimore) ; 100(35): e27104, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34477149

RESUMO

ABSTRACT: To evaluate the diagnostic values of shear wave elastography (SWE) alone and in combination with the Toronto clinical scoring system (TCSS) on diabetic peripheral neuropathy (DPN) in patients with type 2 diabetes mellitus (T2DM).The study included 41 DPN patients, 42 non-DPN patients, and 21 healthy volunteers. Conventional ultrasonography and SWE were performed on the 2 sides of the tibial nerves, and cross-sectional area (CSA) and nerve stiffness were measured. TCSS was applied to all patients. A receiver operating characteristic curve analysis was performed.The stiffness of the tibial nerve, as measured as mean, minimum or maximum elasticity, was significantly higher in patients in the DPN group than the other groups (P < .05). The tibial nerve of subjects in the non-DPN group was significantly stiffer compared to the control group (P < .05). There was no significant difference of the tibial nerve CSA among the 3 groups (P > .05). Mean elasticity of the tibial nerve with a cutoff of 71.3 kPa was the most sensitive (68.3%) and had a higher area under the curve (0.712; 0.602-0.806) among the 3 shear elasticity indices for diagnosing DPN when used alone. When combining SWE with TCSS in diagnosing DPN, the most effective parameter was the EMax, which yielded a sensitivity of 100.00% and a specificity of 95.24%.SWE is a better diagnostic tool for DPN than the conventional ultrasonic parameter CSA, and a higher diagnostic value is attained when combining SWE with TCSS.


Assuntos
Nefropatias Diabéticas/diagnóstico por imagem , Técnicas de Imagem por Elasticidade/normas , Projetos de Pesquisa , Idoso , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/fisiopatologia , Nefropatias Diabéticas/complicações , Técnicas de Imagem por Elasticidade/métodos , Técnicas de Imagem por Elasticidade/estatística & dados numéricos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Doenças do Sistema Nervoso Periférico/classificação , Doenças do Sistema Nervoso Periférico/diagnóstico por imagem , Curva ROC , Reprodutibilidade dos Testes
19.
Europace ; 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-34505884

RESUMO

AIMS: Risk stratification of patients with long QT syndrome (LQTS) represents a difficult task. In 2018, we proposed a granular estimate of the baseline 5-year risk of life-threatening arrhythmias (LAE) for patients with LQTS, based on the genotype (long QT syndrome Type 1, long QT syndrome Type 2, and long QT syndrome Type 3) and the duration of the QTc interval. We sought to externally validate a novel risk score model (1-2-3-LQTS-Risk model) in a geographically diverse cohort from the USA and to evaluate its performance and assess potential clinical implication of this novel model. METHODS AND RESULTS: The prognostic model (1-2-3-LQTS-Risk model) was derived using data from a prospective, single-centre longitudinal cohort study published in 2018 (discovery cohort) and was validated using an independent cohort of 1689 patients enrolled in the International LQTS Registry (Rochester NY, USA). The validation study revealed a C-index of 0.69 [95% confidence interval (CI): 0.61-0.77] in the validation cohort, when compared with C-index of 0.79 (95% CI: 0.70-0.88) in the discovery cohort. Adopting a 5-year risk ≥5%, as suggested by the ROC curve analysis as the most balanced threshold for implantable cardioverter-defibrillator (ICD) implantation, would result in a number needed to treat (NNT) of nine (NNT = 9; 95% CI: 6.3-13.6). CONCLUSION: The 1-2-3-LQTS-Risk model, the first validated 5-year risk score model for patients with LQTS, can be used to aid clinicians to identify patients at the highest risk of LAE who could benefit most from an ICD implant and avoid unnecessary implants.

20.
J Cosmet Dermatol ; 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34496123

RESUMO

BACKGROUND: The nose is located in the middle of the face; therefore, nasal scarring will bring a significant psychological impact on patients. Although there are many treatment methods for depressed scars, these methods have varying degrees of efficacy and all carry certain adverse effects. A better treatment method is urgent to be found. In this study, the effect of micro-plasma radiofrequency technology combined with subcision to treat nasal depressed scars is evaluated. METHODS: 18 Chinese patients with nasal depressed scars participated in this study. All patients received one session of micro-plasma radiofrequency treatment first. 2 months later, subcision combined with micro-plasma radiofrequency technology was performed on them at 6-month intervals, and a total of 2 sessions of combined treatment were performed. Goodman and Baron Scale was used to evaluate nasal scars before treatment and 6 months after the final session. RESULTS: All 18 patients in this study had Grade 4 nasal scars before treatment. 6 months after the end of treatment, 13 patients (72.2%) showed excellent or near total improvement, and 5 patients (27.8%) showed marked improvement. No adverse side effects were observed during treatment. Patient self-evaluation indicated that all patients were satisfied with the cosmetic outcomes. CONCLUSIONS: In this study, we explored a new treatment method for nasal depressed scars. We used micro-plasma radiofrequency technology combined with subcision to treat nasal depressed scars and obtained relatively satisfactory results with no adverse effects.

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