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1.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(8): 885-891, 2019 Aug 28.
Artigo em Chinês | MEDLINE | ID: mdl-31570675

RESUMO

OBJECTIVE: To evaluate the impact of admission diagnosis on seizure outcome in patients with autoimmune epilepsy (AE).
 Methods: We conducted a retrospective study on 40 AE patients at Department of Neurology, Xiangya Hospital, Central South University from Jan. 1st, 2017 to Nov. 21st, 2018. According to their admission diagnosis, these patients were further assigned into 2 groups: An AE diagnosed group and an AE undiagnosed group. Demographic data, clinical characteristics, cerebrospinal fluid (CSF), electroencephalogram, and brain imaging were compared between the 2 groups. Favorable seizure outcome was defined as >50% reduction of seizure frequency at the first follow-up. The impact of admission diagnosis on seizure outcome of AE patients was analyzed.
 Results: The ages of 40 AE patients were (33.23±16.41) years, comprising 19 males and 21 females. No significant difference was found between the AE diagnosed group and the AE undiagnosed group in gender, age, central nervous system-specific Ab profile, CSF, and brain imaging. Duration of symptom onset to Ab detection was significantly longer in the AE undiagnosed group than that in the AE diagnosed group (P<0.01). Duration of symptom onset to immunotherapy was also significantly longer in the AE undiagnosed group than that in the AE diagnosed group (P<0.001). There were more patients with favorable seizure outcome in the AE diagnosed group than the AE undiagnosed group (P<0.05).
 Conclusion: Admission diagnosis for patients with AE is associated with seizure outcome after immunotherapy. For adult-onset epilepsy or epilepsy with unknown etiology, the diagnosis of AE should be considered. Early diagnosis of AE and prompt initiation of immunosuppressive treatment are crucial to increase the likelihood of achieving favorable seizure outcome.


Assuntos
Epilepsia , Adolescente , Adulto , Eletroencefalografia , Feminino , Humanos , Imunoterapia , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Convulsões , Adulto Jovem
2.
J Food Sci ; 84(11): 3296-3302, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31589343

RESUMO

Carbofuran residue in vegetables is a concern to human health. Direct competitive enzyme-linked immunosorbent assay (dcELISA) and dipstick immunoassay were developed in the present study. The dcELISA showed a 50% inhibition concentration (IC50 ) and working range of 1.3 and 0.2 to 7.5 ng/mL, respectively, while the cutoff value of dipstick immunoassay was 20 ng/mL. Applying the two immunoassays, we achieved the goal of rapid screening of carbofuran residue in commercial vegetables with a simple sample processing method. Among 46 leek, 39 potato, and 39 sweet potato samples, carbofuran residue was detected in 22% of the leek samples, and two samples exceeded the maximum residue limit of China (0.02 mg/kg). In addition, carbofuran residue was found at less than 2.5 ng/g in one potato and one sweet potato samples. The residual level of carbofuran measured by immunoassays agreed well with those determined by ultra-performance liquid chromatography tandem mass spectrometry. To ensure food safety and human health, it is greatly necessary and meaningful to monitor carbofuran residue in commercial vegetables. PRACTICAL APPLICATION: Rapid monitoring of carbofuran residue in vegetables is very necessary and important for consumers, regulatory agencies, and food industry.

3.
Biomed Res Int ; 2019: 3726721, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31531351

RESUMO

Identification of protein complex is very important for revealing the underlying mechanism of biological processes. Many computational methods have been developed to identify protein complexes from static protein-protein interaction (PPI) networks. Recently, researchers are considering the dynamics of protein-protein interactions. Dynamic PPI networks are closer to reality in the cell system. It is expected that more protein complexes can be accurately identified from dynamic PPI networks. In this paper, we use the undulating degree above the base level of gene expression instead of the gene expression level to construct dynamic temporal PPI networks. Further we convert dynamic temporal PPI networks into dynamic Temporal Interval Protein Interaction Networks (TI-PINs) and propose a novel method to accurately identify more protein complexes from the constructed TI-PINs. Owing to preserving continuous interactions within temporal interval, the constructed TI-PINs contain more dynamical information for accurately identifying more protein complexes. Our proposed identification method uses multisource biological data to judge whether the joint colocalization condition, the joint coexpression condition, and the expanding cluster condition are satisfied; this is to ensure that the identified protein complexes have the features of colocalization, coexpression, and functional homogeneity. The experimental results on yeast data sets demonstrated that using the constructed TI-PINs can obtain better identification of protein complexes than five existing dynamic PPI networks, and our proposed identification method can find more protein complexes accurately than four other methods.

4.
Cancer ; 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31553494

RESUMO

BACKGROUND: Clinicians are the standard source for adverse event (AE) reporting in oncology trials, despite the subjective nature of symptomatic AEs. The authors designed a pediatric patient-reported outcome (PRO) instrument for symptomatic AEs to support the National Cancer Institute's Common Terminology Criteria for Adverse Events (CTCAE) (the Pediatric PRO-CTCAE). The current study developed a standardized algorithm that maps all possible Pediatric PRO-CTCAE response patterns to recommended CTCAE grades to improve the accuracy of AE reporting in pediatric oncology trials. METHODS: Two rounds of surveys were administered to experienced cancer clinicians across 9 pediatric hospitals. In round 1, pediatric oncologists assigned CTCAE grades to all 101 possible Pediatric PRO-CTCAE response patterns. The authors evaluated clinician agreement of CTCAE grades across response patterns and categorized each response pattern as having high or low agreement. In round 2, a survey was sent to a larger clinician group to examine clinician agreement among a select set of Pediatric PRO-CTCAE response patterns, and the authors examined how clinical context influenced grade assignment. RESULTS: A total of 10 pediatric oncologists participated in round 1. Of the 101 possible patterns, 89 (88%) had high agreement. The Light weighted kappa was averaged across the 10 oncologists (Light kappa = 0.73; 95% CI, 0.66-0.81). A total of 139 clinicians participated in round 2. High clinician agreement remained for the majority of generic response patterns and the clinical context did not typically change grades but rather improved agreement. CONCLUSIONS: The current study provides a framework for integrating child self-reported symptom data directly into mandated AE reporting in oncology trials. Translating Pediatric PRO-CTCAE responses into clinically meaningful metrics will guide future cancer care and toxicity grading.

5.
Ann Vasc Surg ; 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31536795

RESUMO

BACKGROUND: This study aims to present the performance data on stent-graft and multilayer bare stents (MBS) joint technique in the treatment of high-risk thoracoabdominal aortic aneurysm (TAAA). METHODS: From May 2012 to December 2015, 8 selective TAAA cases (ages 46-75 years) ineligible for surgical repair underwent the stent-graft and MBS joint procedure, and were closely followed up for a median of 32 months (range 14-58). Using computed tomography images, the aneurysm size, luminal blood flow diameter, and the covered visceral branches were analyzed. RESULTS: Technical success was achieved in all patients (100%, 8/8). Twenty-four visceral branches were covered by MBS in total. There was no complication or death during hospital stay. During follow-up period, no death or complication occurred. Aneurysm shrinkage (maximum diameter decrease ≥5 mm) was observed in 7 patients. No aneurysm expansion was observed. Total aneurysm sac thrombosis was observed in all patients. The majority of covered side branches (23/24) were successfully preserved. No visceral ischemia or bleeding complications was observed during follow-up. CONCLUSIONS: Total endovascular repair of TAAA using stent-graft and MBS joint technique may be a safe and effective alternative in high surgical risk patients. More approving clinical evidences about the safety and efficacy of this procedure are anticipated.

6.
Biosci Rep ; 39(9)2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31427483

RESUMO

Background: The liver-specific microRNA-122 (miR-122) has been demonstrated as a powerful and promising biomarker of hepatic diseases. However, the researches on the accuracy of miR122 detection in chronic viral hepatitis have been inconsistent, leading us to conduct this meta-analysis to systematically summarize the diagnostic value of circulating miR-122 in patients with hepatitis B virus (HBV) and/or hepatitis C virus (HCV)-associated chronic viral hepatitis.Methods: A comprehensive literature search (updated to January 30, 2019) in PubMed, Cochrane library, EMBASE, CNKI, Wanfang, and CQVIP databases was performed to identify eligible studies. The sensitivity (SEN), specificity (SPE), positive and negative likelihood ratios (PLR and NLR), diagnostic odds ratio (DOR), and area under the curve (AUC) were pooled to explore the diagnostic performance of circulating miR-122. Subgroup and threshold effect analysis were further carried out to explore the heterogeneity.Results: Overall, 15 studies were finally included in this meta-analysis according to the exclusion and inclusion criteria. The pooled estimates indicated a moderately high diagnostic accuracy for circulating miR-122, with a sensitivity of 0.92 [95% confidence interval (CI), 0.86-0.95], a specificity of 0.84 (95% CI, 0.78-0.89), a PLR of 5.7 (95% CI, 4.7-8.1), a NLR of 0.1 (95% CI, 0.06-0.18), a DOR of 57 (95% CI 25-129), and an AUC of 0.93 (95% CI, 0.91-0.95). The subgroup analysis demonstrated that diagnostic accuracy was better for HCV-associated chronic viral hepatitis patients and non-Chinese compared with other subgroups. In addition, we found that serum might be a more promising matrix for detecting the expression of miR-122 than plasma.Conclusions: Our results demonstrated that circulating miR-122 have a relatively high diagnostic value for chronic viral hepatitis detection, especially in the patients with HCV-associated chronic viral hepatitis. However, further large cohort studies are still required to confirm our findings.

7.
J Cell Mol Med ; 23(10): 7010-7020, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31436914

RESUMO

Mild hypothermia and its key product, cold-inducible protein RBM3, possess robust neuroprotective effects against various neurotoxins. However, we previously showed that mild hypothermia fails to attenuate the neurotoxicity from MPP+ , one of typical neurotoxins related to the increasing risk of Parkinson disease (PD). To better understand the role of mild hypothermia and RBM3 in PD progression, another known PD-related neurotoxin, rotenone (ROT) was utilized in this study. Using immunoblotting, cell viability assays and TUNEL staining, we revealed that mild hypothermia (32°C) significantly reduced the apoptosis induced by ROT in human neuroblastoma SH-SY5Y cells, when compared to normothermia (37°C). Meanwhile, the overexpression of RBM3 in SH-SY5Y cells mimicked the neuroprotective effects of mild hypothermia on ROT-induced cytotoxicity. Upon ROT stimulation, MAPK signalling like p38, JNK and ERK, and AMPK and GSK-3ß signalling were activated. When RBM3 was overexpressed, only the activation of p38, JNK and ERK signalling was inhibited, leaving AMPK and GSK-3ß signalling unaffected. Similarly, mild hypothermia also inhibited the activation of MAPKs induced by ROT. Lastly, it was demonstrated that the MAPK (especially p38 and ERK) inhibition by their individual inhibitors significantly decreased the neurotoxicity of ROT in SH-SY5Y cells. In conclusion, these data demonstrate that RBM3 mediates mild hypothermia-related neuroprotection against ROT by inhibiting the MAPK signalling of p38, JNK and ERK.

8.
Comput Biol Med ; 111: 103333, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31376777

RESUMO

Identifying protein complexes in static protein-protein interaction (PPI) networks is essential for understanding the underlying mechanism of biological processes. Proteins in a complex are co-localized at the same place and co-expressed at the same time. We propose a novel method to identify protein complexes with the features of joint co-localization and joint co-expression in static PPI networks. To achieve this goal, we define a joint localization vector to construct a joint co-localization criterion of a protein group, and define a joint gene expression to construct a joint co-expression criterion of a gene group. Moreover, the functional similarity of proteins in a complex is an important characteristic. Thus, we use the CC-based, MF-based, and BP-based protein similarities to devise functional similarity criterion to determine whether a protein is functionally similar to a protein cluster. Based on the core-attachment structure and following to seed expanding strategy, we use four types of biological data including PPI data with reliability score, protein localization data, gene expression data, and gene ontology annotations, to identify protein complexes. The experimental results on yeast data show that comparing with existing methods our proposed method can efficiently and exactly identify more protein complexes, especially more protein complexes of sizes from 2 to 6. Furthermore, the enrichment analysis demonstrates that the protein complexes identified by our method have significant biological meaning.

9.
Neuroreport ; 30(12): 842-846, 2019 08 14.
Artigo em Inglês | MEDLINE | ID: mdl-31283708

RESUMO

A known cause of seizure-induced respiratory arrest is the deficiency in serotonergic neurotransmission. Tryptophan hydroxylase-2 (TPH2) is the rate-limiting enzyme of central serotonin (5-hydroxytryptamine) synthesis which converts l-tryptophan to 5-hydroxytryptophan. A recent study revealed a reduction in TPH2 protein expression in the brainstems of DBA/1 mice that developed recurrent seizure-induced respiratory arrest, whereas the activity of this protein was unexplored. Thus this study aims to investigate the association between intrinsic 5-hydroxytryptamine synthesis in the brainstem and the susceptibility for sudden unexpected death in epilepsy in DBA/1 mice. The effect of LY393558, a potent 5-hydroxytryptamine reuptake inhibitor with 5-HT1B/1D receptor antagonist properties, on seizure-induced respiratory arrest evoked by acoustic stimulation was also examined in DBA/1 mice. ELISA results showed significantly decreased TPH2 activity in the brainstems of untreated DBA/1 mice than that of C57BL/6J mice. Moreover, the concentrations of 5-hydroxytryptamine, 5-hydroxytryptophan and 5-HIAA in the brainstems of DBA/1 mice with or without acoustic stimulation were significantly lower than that of C57BL/6J mice. Acute administration of LY393558 also significantly reduced seizure-induced respiratory arrest in DBA/1 mice. These observations provide novel evidences for the hypothesis that 5-hydroxytryptamine deficiency might be a potential cause of seizure-induced respiratory arrest.

10.
J Public Health Dent ; 79(3): 253-263, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31209896

RESUMO

OBJECTIVES: The purpose of this study was to develop an instrument in English (ENG) and Spanish (SPA) for use in assessing perceptions of dental home characteristics among low-income adults. METHODS: An expert panel selected 21 items from a pool of 81 items mapped to domains in the American Academy of Pediatrics' medical home. Pilot testing with mothers (n = 795) of children in Early Head Start (EHS) resulted in an expanded 36-item scale, which was field-tested in interviews with ENG- (NENG = 665) and SPA-speakers (NSPA = 116). An exploratory factor analysis of 23 usable items was carried out in an EHS reference group (NENG-EHS = 272). The selected model was evaluated by confirmatory factor analysis (CFA) in two other subgroups (NENG-NonEHS = 393; NSPA = 116). We tested measurement and structural invariances using a multiple-group approach. Known-groups validity was examined by testing factor mean differences between two groups defined by whether or not the mother used a single dental office. RESULTS: A three-factor CFA model with 10 items was retained for its close fit for all three subgroups. Invariance tests found the two ENG subgroups to be homogenous and were combined. Several item and factor parameters in the SPA group differed from the combined ENG group. The proposed dental home measure demonstrated good known-group validity in that people who used the same dental office reported better dental care experiences. CONCLUSIONS: An instrument to measure the dental home concept among low-income adults was developed with three domains (accessible-comprehensive, compassionate, and health literate care) that demonstrated good validity and reliability.

11.
Drug Test Anal ; 11(9): 1444-1452, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31150570

RESUMO

Dihydroartemisinin (DHA) and piperaquine (PPQ) are two drugs used in an artemisinin-based combination therapy (ACT). The circulation of counterfeit antimalarial drugs demands the development of simple, point-of-care (POC) tests for monitoring drug quality. Here we aimed to design an antibody-based lateral flow dipstick assay for simultaneous quality control of DHA and PPQ. To obtain a monoclonal antibody (mAb) for PPQ, one structural unit of the symmetric PPQ molecule was used to derive a carboxylic acid for linkage to a carrier protein as immunogen. Screening of hybridoma cells identified an mAb 4D112B2 that reacted with the PPQ-based immunogen. A highly-sensitive icELISA was designed based on this mAb, which showed 50% inhibition concentration of PPQ at 1.66 ng/mL and a working range of 0.35 - 7.40 ng/mL. The mAb showed 10.2, 15.9 and 30.4% cross reactivity to hydroxychloroquine sulfate, chloroquine and amodiaquine, respectively. No cross reactivity was observed to lumefantrine, mefloquine artemisinin and its derivatives. Using our previous DHA dipstick design, a lateral flow dipstick for simultaneous analysis of PPQ and DHA was developed. The indicator ranges for PPQ and DHA were 2 - 5 µg/mL and 250 - 500 ng/mL, respectively. The dipstick was used to semi-quantitatively analyze PPQ and DHA content in commercial ACT drugs, which produced agreeable results to those determined by high-performance liquid chromatography. This combination dipstick makes it a potential POC device for quality control of the two active ingredients in a commonly used ACT.

12.
Environ Pollut ; 250: 437-446, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31026690

RESUMO

In this study, antibiotic resistance to macrolide-lincosamide-streptogramin B (MLSB) antibiotics in total microbial community in surface water in a coastal urban city was measured using a modified fluorescence in situ hybridization (FISH) technique. This FISH technique quantified the rate of antibiotic resistance to MLSB antibiotics through targeting methylation site of A2058 of 23S rRNAs resulting from expressed erythromycin ribosome methylation (erm) genes. Correlations between the rates of MLSB resistance measured by FISH and macrolide concentrations was stronger than that between the relative abundance of erm genes and macrolide concentrations, especially in residential areas where the main detected antibiotics were macrolides. These results suggest that trace levels of antibiotics in environmental waters, which was as low as 40 ng L-1, may still play important roles in the development and spread of antibiotic resistance. Additionally, methylation as a result of erm gene expression, instead of erm gene abundance, was a better indicator of selective pressure of trace level macrolides. The rates of MLSB resistance varied significantly among land use types, suggesting that anthropogenic activities are important factors to select for erm gene expression in the environment. Microbial community analysis of representative surface water samples showed that relatively high rates of MLSB resistance were observed in Alphaproteobacteria (42%), Acidobacteria (36%), Bacteroidaceae (32%), Chloroflexi (27%), and Betaproteobacteria (20.2%).


Assuntos
Antibacterianos/análise , Resistência Microbiana a Medicamentos/genética , Monitoramento Ambiental , Microbiologia da Água , Poluentes Químicos da Água/análise , Eritromicina , Genes Microbianos , Hibridização in Situ Fluorescente , Lincosamidas/análise , Macrolídeos/análise , Testes de Sensibilidade Microbiana , Estreptogramina B/análise , Estreptogramina Grupo B/análise , Virginiamicina/análise
13.
J Pharm Sci ; 108(9): 3074-3081, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30981753

RESUMO

A series of novel low-toxic hepatoma cell-targeting lipid materials were designed and synthesized, in which monogalactose, digalactose, and galactose-biotin were used as targeting moieties and hydrophilic heads while stearate was used as hydrophobic tail (Mono-Gal-ST, Di-Gal-ST, and Gal-Biotin-ST). The corresponding galactose-biotin-modified liposomes (Mono-Gal-LPs, Di-Gal-LPs, and Gal-Biotin-LPs) and conventional liposomes (LPs) were prepared. These galactose-biotin-modified liposomes can distinguish hepatoma cells from other tissue cells owing to the recognition of asialoglycoprotein receptor by galactose group. Moreover, the ability of liposomes to distinguish hepatoma cells from normal hepatocytes follows a trend of LPs < Mono-Gal-LPs < Di-Gal-LPs < Gal-Biotin-LPs, which is attributed to the cluster glycoside effect and the synergistic effect of galactose and biotin. In addition, the endocytosis of these galactose-biotin-modified liposomes were competitively inhibited by galactose, further confirming these liposomes entered hepatoma cells via asialoglycoprotein receptor-mediated pathway.

14.
Med Sci Monit ; 25: 2527-2534, 2019 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-30953435

RESUMO

BACKGROUND There are few studies on distributions or morphological measurements for bony spurs form at the attachment points of the ligaments and tendons on the coracoid process. The aim of this study was to investigate their most common sites and morphological characteristics, and to propose possible reasons. MATERIAL AND METHODS Scapulae with bony spurs on the coracoid process were selected from 377 intact and dry Chinese scapulae. The distribution, height, and transverse and longitudinal diameter of the bony spurs were measured in each coracoid process. RESULTS We selected 71 scapulae, 36 left and 35 right, that had bony spurs, from 377 scapulae. The bony spurs were most commonly located at the attachment point of the superior transverse scapular ligament (STSL) (31, 23.66%), while the trapezoid ligament (TL) accounted for the smaller proportion (8, 6.11%). The TSL was the highest, with the minimum transverse and longitudinal diameter, while the TL had the greatest transverse and longitudinal diameters. Only the TSL and TL had a statistically significant difference between the left and the right bony spur regarding the longitudinal diameter (P<0.05). CONCLUSIONS Bony spurs are more likely to form at the attachment points of ligaments and tendons on the coracoid process, which has a greater risk of traction injuries or attachment points avulsion fractures.


Assuntos
Processo Coracoide/patologia , Osteófito/epidemiologia , Osteófito/patologia , Grupo com Ancestrais do Continente Asiático/genética , Cadáver , China/epidemiologia , Humanos , Ligamentos Articulares , Escápula/anatomia & histologia , Escápula/patologia , Articulação do Ombro
15.
J Gastrointest Surg ; 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30937708

RESUMO

BACKGROUND: Intestinal autotransplantation (IATx) is a novel surgical technique for neoplasms arising from the pancreas, duodenum, mesentery, or retroperitoneum with involvement of the superior mesenteric artery (SMA). The value of this aggressive procedure remains to be defined. We describe its surgical indications, postoperative complications, and clinical outcomes after IATx. METHODS: Fifteen patients aged 20 to 67 years (mean 44.9 years) underwent IATx in our program from January 2011 to January 2018. In all patients, selection and harvesting of a healthy bowel autograft were initially carried out, and an extended en bloc resection of neoplasms was performed afterward. RESULTS: Of the 15 patients, there was one early death from a pancreatic leak and two late deaths either from disease recurrence or sudden cardiac arrest. Ten patients developed 23 postoperative complications. Of these, one patient lost his bowel autograft due to arterial thrombosis 48 h later. Delayed gastric emptying, pleural effusions, pancreatic fistula, and relaparotomy were the most common complications. In our series, four of nine patients with invasive malignant neoplasms had evidence of disease recurrence at 13, 13, 16, and 18 months after IATx. At a median follow-up of 29.9 months, 11 patients undergoing successful IATx remained alive with a well-functioning bowel graft. CONCLUSION: Our results indicate that IATx is technically feasible with acceptable perioperative morbidity and mortality. This procedure should be considered in selected patients presenting with locally invasive neoplasms involving the SMA.

16.
Complement Ther Clin Pract ; 35: 22-32, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31003662

RESUMO

BACKGROUND: and purpose: The benefits of Kinesio taping (KT) in post-stroke rehabilitation have not been determined. This study aimed to evaluate its effects on lower-extremity rehabilitation in patients after a stroke. METHODS: A literature search was performed using EBSCOhost, Embase, Physiotherapy Evidence Database (PEDro), PubMed, Cochrane, Web of Science, China National Knowledge Infrastructure (CNKI), SinoMed, and Wanfang Data through June 2018. Randomized controlled trials (RCTs) on the use of KT during lower-extremity, post-stroke rehabilitation were selected. Meta-analysis was conducted. RESULTS: A total of 14 RCTs of low to moderate quality were reviewed and included 783 participants. Results indicated that KT significantly improved patients' lower extremity spasticity, motor function, balance, ambulation, gait parameters, and daily activities, with few adverse effects. CONCLUSION: KT may have positive effects on lower-extremity, post-stroke rehabilitation. Due to the limited number and quality of the research, additional studies are needed to identify KT benefits.


Assuntos
Fita Atlética , Extremidade Inferior , Modalidades de Fisioterapia , Reabilitação do Acidente Vascular Cerebral/métodos , Acidente Vascular Cerebral/complicações , Atividades Cotidianas , Adolescente , Adulto , Marcha , Humanos , Espasticidade Muscular/etiologia , Espasticidade Muscular/prevenção & controle , Equilíbrio Postural , Acidente Vascular Cerebral/fisiopatologia , Caminhada
17.
Food Chem ; 291: 132-138, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31006451

RESUMO

trans-Resveratrol, a natural polyphenol present in grapes, has many beneficial effects to human health. However, measurement of trans-resveratrol is technically complicated and time-consuming. In the present study, we obtained a sensitive and specific monoclonal antibody (mAb), namely 3C9, against trans-resveratrol. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed, with 50% inhibitory concentration and working range of 1.0 ng/mL and 0.19-4.9 ng/mL, respectively. A lateral flow immunoassay (LFIA) based on 3C9 was also developed for the semiquantitative detection of trans-resveratrol in an indicator range of 50-100 ng/mL. Average recoveries of trans-resveratrol spiked in red and green grape berries samples were respectively 88-107% and 83-102% by icELISA. The icELISA and LFIA were applied for determination of trans-resveratrol in grape berries. The results were highly consistent with those determined by high-performance liquid chromatography analysis (R2 = 0.9997). Therefore, we conclude that the immunoassay methods are suitable for the large-scale screening of trans-resveratrol in grape quality breeding.


Assuntos
Ensaio de Imunoadsorção Enzimática , Resveratrol/análise , Vitis/química , Animais , Anticorpos Monoclonais/imunologia , Cromatografia Líquida de Alta Pressão , Feminino , Frutas/química , Frutas/metabolismo , Isomerismo , Camundongos , Camundongos Endogâmicos BALB C , Resveratrol/imunologia , Vitis/metabolismo
18.
Biomater Sci ; 7(6): 2430-2439, 2019 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-30933194

RESUMO

Coaxial electrospinning is a novel technique for producing core-shell nanofibers that provide a robust structure and deliver hydrophilic bioactive agents. Optimization of parameters used in the coaxial electrospinning process allows for the fabrication of uniform and bead-free polyvinyl alcohol (PVA)/poly (l-lactic acid) (PLLA) core-shell nanofibers. Herein, a cold atmospheric plasma (CAP) process was used to enhance the surface features of electrospun core-shell nanofibers for increased surface pore size and altered surface hydrophilicity. After CAP treatment, the scaffolds' water contact angle was reduced from 110° to 50° and its protein and water adsorption were significantly elevated. The changes in hydrophilicity and improved scaffold surface area dramatically enhanced cell attachment and proliferation of fibroblasts and osteoblasts. Also, the increased levels of alkaline phosphatase (ALP) activity, total protein content and calcium deposition from mesenchymal stem cells (MSCs) indicated a higher osteoinductivity of the CAP-modified nanofibrous scaffold. Most importantly, the increased nanofiber surface pore size induced by the CAP treatment further contributed to significant variations in drug release profiles. The CAP-treated scaffolds showed more rapid release kinetics compared to untreated scaffolds, which eventually led to complete drug release. These results indicated that the CAP-treated and bioactive protein-loaded core-shell nanofibers could be a valuable regenerative medicine and drug delivery system for improved bone tissue engineering.

19.
Rehabil Nurs ; 44(2): 78-86, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30830883

RESUMO

PURPOSE: The aim of the study was to evaluate the effectiveness of a modified short-term mindfulness-based intervention on improving the mindfulness, comfort, and ambulation ability of stroke survivors undergoing inpatient rehabilitation in Wuhan, China. DESIGN: A two-group, nonrandomized, nonconcurrent design was used. METHOD: Participants undergoing inpatient rehabilitation were nonrandomly divided into control group (n = 25) and intervention group (n = 25) to avoid possible ethical discrimination as well as interaction among participants. The control group received routine care; the intervention group received a 2-week mindfulness-based intervention, which included weekly 1.5-hour group practice, individual daily practice, and routine care. Data were collected using questionnaires and assessments of ambulation before and after 2 weeks of observation. FINDINGS: Improvement on the Mindful Attention Awareness Scale score, the overall score of the Shortened General Comfort Questionnaire, and the scores of the physical, psychospiritual, and sociocultural subscales in the intervention group was greater than those in the control group (p < .05). No significant difference (p > .05) was observed between the two groups when comparing scores of the environmental subscale of Shortened General Comfort Questionnaire, Berg Balance Scale, 10-Meter Walk Test, and Functional Ambulation Classification scale. CONCLUSIONS AND CLINICAL RELEVANCE: A 2-week mindfulness-based intervention can significantly improve stroke survivors' mindfulness and comfort but does not significantly affect ambulation ability.


Assuntos
Atenção Plena/métodos , Acidente Vascular Cerebral/psicologia , Sobreviventes/psicologia , Adulto , Idoso , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Atenção Plena/normas , Psicometria/instrumentação , Psicometria/métodos , Centros de Reabilitação/organização & administração , Acidente Vascular Cerebral/complicações , Reabilitação do Acidente Vascular Cerebral/métodos , Inquéritos e Questionários
20.
Mater Sci Eng C Mater Biol Appl ; 99: 685-695, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30889742

RESUMO

The green synthesis of biomaterials is of significant interest as it enables the safe and sustainable preparation of noble metallic nanoparticles for medical applications. Microalgae polysaccharides have received attention due to their outstanding properties such as biocompatibility, biodegradability and low cost. In addition, due to their variety of remarkable biological and physicochemical properties, polysaccharide-based nanoparticles have advantageous features yet to be explored. The primary objective of the current research was to investigate exopolysaccharides isolated from green microalgae Botryococcus braunii (EPBb) and Chlorella pyrenoidosa (EPCp), as both reducing and stabilizing agents, for the green synthesis of silver nanoparticles (AgNPs). Their antibacterial activity towards Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Escherichia coli), and antibiotic-resistant bacteria (methicillin-resistant Staphylococcus aureus) was studied, as well as their cytotoxicity to human dermal fibroblasts. The presently synthesized AgNPs were spherical in shape and exhibited characteristic surface plasmon resonance at 430 nm. The main population had a particle size which ranged between 5 and 15 nm as analyzed by transmission electron micrographs. Zeta potentials averaged -51.81 ±â€¯3.01 mV using EPBb and -12.16 ±â€¯2.41 mV using EPCp. More importantly, AgNPs possessed strong antibacterial activity in a dose-dependent manner, even against drug-resistant bacteria. The enhanced antibacterial activity of these particles is explained due to extensive reactive oxygen species generation and bacterial cell membrane damage. In contrast, such AgNPs were not cytotoxic at the same therapeutic range to fibroblasts (0.5-10.0 µg/mL). In summary, these results showed that polysaccharide-capped AgNPs have a strong potential for numerous medical applications, such as antibacterial agents in pharmaceutical and biomedical areas.


Assuntos
Antibacterianos/farmacologia , Nanopartículas Metálicas/química , Microalgas/química , Polissacarídeos/farmacologia , Prata/farmacologia , Morte Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Escherichia coli/ultraestrutura , Fibroblastos/citologia , Humanos , Nanopartículas Metálicas/ultraestrutura , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Espécies Reativas de Oxigênio/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier
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