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1.
Sci Total Environ ; 753: 142194, 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33207455

RESUMO

Phyllospheric microbes play a crucial role in the biological decomposition of plant litter in wetland ecosystems. Previous studies have mainly focused on single stages of decomposition process, and to date there have been no reports on dynamic changes in the composition of phyllospheric microbes during the multiple stages of decomposition from living plant to death. Here we investigated fungal and bacterial community succession in the leaf litter of Schoenoplectus tabernaemontani, a wetland plant species using sequencing of the both fungal ITS and bacterial 16S genes. Our results revealed that, over the whole period of decomposition, the fungal communities underwent more distinct succession than did the bacterial communities. Proteobacteria dominated throughout the entire period, while, across different decomposition stages, the Ascomycete fungi were gradually replaced by the Ciliophora and Rozellomycota as the dominant fungi. Network analysis revealed higher degrees of species segregation and shorter average path lengths between species of fungi compared with species of bacteria. This suggests that fungal communities may harbor more niches and functional diversity and are potentially more susceptible to external interference than are bacterial communities. During decomposition, the contents of leaf cellulose, hemicellulose and lignin in the litter were significantly (p < 0.01) correlated with the fungal communities, and abiotic factors accounted for 89.8% of the total variation in the fungal communities. In contract, abiotic factors only explained 6.10% of the total variation in bacterial communities, suggesting external environments as drivers of fungal community succession. Overall, we provide evidence that the complex litter decay in wetlands is the result of a dynamic cross-kingdom succession, and this process is accompanied by distinct phyllospheric fungal community dynamics.


Assuntos
Microbiota , Micobioma , Bactérias/genética , Ecossistema , Fungos , Folhas de Planta , Microbiologia do Solo , Áreas Alagadas
2.
Plant Dis ; 2020 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-33143563

RESUMO

Huangjing (Polygonatum sibiricum) is a medicinal plant widely distributed in China, Japan, and Korea. The dried rhizome of Huangjing has been reported to have many pharmacological applications and biological activities, such as antioxidants, immunity enhancement, anti-fatigue, anti-osteoporosis, and anti-aging activity (Cui et al., 2018). In June 2018, we observed some wilted Huangjing plants in commercial plantings in Shuicheng, Guizhou, China (26.22 N, 104.76 E). Symptoms began as moderate to severe wilting of stems and necrosis of leaves, followed by the death of plants. The collar rot appeared on the stem near to the soil. When incubated at 28°C and 100% relative humidity (RH) for 8 to 10 days, the infected stem produced brown sclerotia. We picked the sclerotia and cultured them on potato dextrose agar (PDA) supplemented with 50 µg/ml of streptomycin. The hyphal tips generated by the sclerotia was isolated under microscopic field and transferred to the fresh PDA. Three isolates (HJ-1, HJ-6 and HJ-10) came from the hyphal tips formed the typical clamp connection structure at 6-7 days post-incubation and the sclerotia of them were white and the late ones turned dark brown. The matured sclerotia were globular, 1.5 to 3.3 mm (avg. 2.2) in diameter. The morphologic observation revealed that three isolates were consistent with Athelia rolfsii (Paul et al., 2017). To further confirm the fungal species, the ribosomal internal transcribed spacer (ITS) sequences were amplified and sequenced. Primers and PCR amplification were referenced as previously described (Paul et al., 2017). The sequences were compared to type sequences in GenBank. The ITS sequences (GenBank accession MT478452, MT949696 and MT949697) of the isolates (HJ-10, HJ-1 and HJ-6) were 99% identical with strain 13M-0091 (GenBank accession KT222898) of A. rolfsii, respectively (Paul et al., 2017). A maximum likelihood tree was constructed using MEGA-X version 10.1.6 (Kumar et al., 2018) based on the ITS sequences of the three strains (HJ-10, HJ-1 and HJ-6) and that of Athelia spp. previously deposited in GenBank (Paul et al., 2017). Phylogenetic analysis showed that the isolates (HJ-10, HJ-1 and HJ-6) belong to the A. rolfsii clade. Based on morphology and DNA sequencing, the isolates (HJ-10, HJ-1 and HJ-6) were identified as A. rolfsii. To verify pathogenicity, Huangjing seedlings were inoculated with colonized agar discs of the isolates. Additional Huangjing plants inoculated with uncolonized agar discs were used as the control. After inoculation, Huangjing seedlings were moved to the inoculation chamber under high humidity and 28°C for 3 days and then transferred to a greenhouse. The typical wilting symptoms appeared 8 days after inoculation and were similar to those observed in the field, while control plants remained symptomless. The causing agents were isolated from the lesions and the ITS sequences of them were sequenced again. The alignment analysis of the ITS sequences showed the causing agents are consistent with the original isolates. These studies fulfilled Koch's postulates. To our knowledge, this is the first report of A. rolfsii causing stem rot on Huangjing.

3.
Mol Pharm ; 2020 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-33201717

RESUMO

Curcumin (CUR), a polyphenol derived from turmeric, exhibits anticancer and anti-inflammatory properties. However, it has poor water solubility, stability, and oral bioavailability. To overcome these limitations, lipid-polyester mixed nanoparticles (NPs) embedded in enteric polymer-EudragitL100-55(Eu) were formulated (CUR-NPs-Eu). NPs composed of mPEG-b-PCL have a hybrid core made up of middle chain triglyceride (MCT) and poly(ε-caprolactone) (PCL) for enhancing drug loading. The CUR-NPs with MCT content of 10% had a particle size of 121.2 ± 16.8 nm, ζ potential of -16.25 ± 1.38 mV, drug loading of 9.8%, and encapsulation efficiency of 87.4%. The transport of the CUR-NPs-Eu across Caco-2 monolayers is enhanced compared with CUR alone (1.98 ± 0.94 × 10-6 of curcumin versus 55.43 ± 6.06 × 10-6 cm/s of curcumin-loaded NPs) because of the non-disassociated nanostructure during absorption. The absolute bioavailability of CUR-NPs-Eu was 7.14%, which was drastically improved from 1.08% of the CUR suspension (CUR-Sus). Therefore, in the xenograft 4T1 tumor-bearing mice, increased drug accumulation in heart and tumor was noticed because of enhanced oral bioavailability of CUR. The chemosensitizing effect of CUR was attributed to its NF-κB reduction effect (148 ± 11.83 of DOX alone versus 104 ± 8.71 of combined therapy, ng/g tissue). The cardioprotective effect of CUR was associated with maintenance of cardiac antioxidant enzyme activity and down-regulation of NF-κB. This study provided a partial illustration of the mechanisms of chemosensitizing and cardioprotective effects of CUR utilizing the oral availability promotion effect brought by the NPs-Eu formulation. And these results further demonstrated that the capability of this NPs-Eu system in oral delivery of poorly soluble and poorly permeable drugs.

4.
Biomark Med ; 14(15): 1405-1413, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33151094

RESUMO

Aim: Data from 124 rheumatoid arthritis (RA) patients and 69 healthy controls were collected. Materials & methods: ELISA was performed to detect serum FABP4 levels. Results: FABP4 level was elevated in RA patients and positively associated with 28-joint disease activity score, high-sensitivity C-reactive protein, erythrocyte sedimentation rate, total cholesterol, triglyceride and low-density lipoprotein cholesterol. Additionally, the area under the receiver operating characteristic curve for FABP4 was 0.685 for RA patients versus healthy controls (p = 0.001). RA patients were separated into low, moderate and high disease activity based on 28-joint disease activity score. The area under the receiver operating characteristic value was 0.877 for RA patients with high disease activity versus healthy controls (p < 0.001). Conclusion: FABP4 was associated with disease activity in RA patients.

6.
Pak J Pharm Sci ; 33(3): 1139-1146, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-33191240

RESUMO

Neonatal acute respiratory distress syndrome (ARDS) is a serious stage of acute lung injury (ALI) which can be treated by exogenous surfactant. The aim of this study was to explore the clinical efficacy of two different doses of Poractant alfa (Curosurf®) for treating neonatal ARDS and to perform an economic evaluation. Fifty-four patients were divided into Group A (high dose) and Group B (low dose). Pharmacoeconomic evaluation was performed on the two groups regarding the treatment expenses, and the output was the cure rate and complication rate. There were significant differences between Group A and Group B for the duration of receiving oxygen therapy in moderate cases (6.4±3.5d:8.9±2.6d) (P<0.05) and severe cases (10.0±2.6d:14.8±1.3d) (P<0.05). There were significant differences between them for the duration of undergoing mechanical ventilation in severe cases (1.7±2.3d:5.5±2.4d) (P=0.01). There was a significant difference between Group A and Group B for hospitalization expenses in severe cases (P<0.05). There were no significant differences between them in all types of cases for the cure rate (P>0.05). A high dose of Curosurf had an advantage in treating neonatal ARDS, especially in severe cases, with lower final costs and better effects.

7.
BMJ Glob Health ; 5(11)2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33184065

RESUMO

INTRODUCTION: The COVID-19 pandemic caused a healthcare crisis in China and continues to wreak havoc across the world. This paper evaluated COVID-19's impact on national and regional healthcare service utilisation and expenditure in China. METHODS: Using a big data approach, we collected data from 300 million bank card transactions to measure individual healthcare expenditure and utilisation in mainland China. Since the outbreak coincided with the 2020 Chinese Spring Festival holiday, a difference-in-difference (DID) method was employed to compare changes in healthcare utilisation before, during and after the Spring Festival in 2020 and 2019. We also tracked healthcare utilisation before, during and after the outbreak. RESULTS: Healthcare utilisation declined overall, especially during the post-festival period in 2020. Total healthcare expenditure and utilisation declined by 37.8% and 40.8%, respectively, while per capita expenditure increased by 3.3%. In a subgroup analysis, we found that the outbreak had a greater impact on healthcare utilisation in cities at higher risk of COVID-19, with stricter lockdown measures and those located in the western region. The DID results suggest that, compared with low-risk cities, the pandemic induced a 14.8%, 26.4% and 27.5% reduction in total healthcare expenditure in medium-risk and high-risk cities, and in cities located in Hubei province during the post-festival period in 2020 relative to 2019, an 8.6%, 15.9% and 24.4% reduction in utilisation services; and a 7.3% and 18.4% reduction in per capita expenditure in medium-risk and high-risk cities, respectively. By the last week of April 2020, as the outbreak came under control, healthcare utilisation gradually recovered, but only to 79.9%-89.3% of its pre-outbreak levels. CONCLUSION: The COVID-19 pandemic had a significantly negative effect on healthcare utilisation in China, evident by a dramatic decline in healthcare expenditure. While the utilisation level has gradually increased post-outbreak, it has yet to return to normal levels.


Assuntos
Infecções por Coronavirus/epidemiologia , Gastos em Saúde/estatística & dados numéricos , Acesso aos Serviços de Saúde , Aceitação pelo Paciente de Cuidados de Saúde/estatística & dados numéricos , Pneumonia Viral/epidemiologia , Betacoronavirus , China/epidemiologia , Humanos , Pandemias
8.
J Ethnopharmacol ; : 113581, 2020 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-33189841

RESUMO

BACKGROUND: and ethnopharmacological relevance: As the major side effect of radiotherapy or chemotherapy, myelosuppression usually leads to anemia, hemorrhage, immunosuppression, and even fatal infections, which may discontinue the process of cancer treatment. As a result, more and more attention is paid to the treatment of myelosuppression. Ginseng, root of Panax ginseng Meyer (Panax ginseng C. A. Mey), is considered as the king of herbs in the Orient, particularly in China, Korea and Japan. Ginsenosides, the most important active ingredients of ginseng, have been shown to have a variety of therapeutic effects, such as neuroprotective, anti-cancer and anti-diabetic properties. Considering that ginsenosides are closely associated with the pathogenesis of myelosuppression, researchers have carried out a few experiments on ginsenosides to attenuate myelosuppression induced by chemotherapy or radiotherapy in recent years. AIM OF THE STUDY: To summarize previous studies about the effects of ginsenosides on alleviating myelosuppression and the mechanisms of action. METHODS: Literatures in this review were searched in PubMed, China National Knowledge Infrastructure (CNKI), Web of Science, and ScienceDirect. RESULTS: Ginsenosides play an important role in relieving myelosuppression predominantly by restoring hematopoiesis and immunity. CONCLUSION: Ginsenosides might be potential candidates for the treatment of myelosuppression induced by chemotherapy or radiotherapy.

9.
J Int Med Res ; 48(11): 300060520966877, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33167762

RESUMO

OBJECTIVE: To review our experiences on clinical management of pregnancies with positive noninvasive prenatal testing (NIPT) results for rare autosomal aneuploidies (RAAs) at a single center. METHODS: We performed a retrospective study and reviewed data from 18,016 pregnancies undergoing NIPT at a single center in China from March 2017 to February 2020. Depending on the patient's choice, women with positive screening results for RAAs underwent chromosomal microarray analysis for invasive prenatal diagnosis. RESULTS: Thirty-three positive cases for RAAs were identified, with a positive screening rate of 0.18%. The most common RAA was trisomy 7 (33.3%), while trisomies for other chromosomes were less frequent. Monosomies involving chromosomes 16, 14, and 22 were observed. Twenty-eight cases of RAAs underwent invasive diagnosis. Abnormal pregnancy outcomes were observed in four cases, including true fetal mosaicism (n=1), partial uniparental disomy (n=1), miscarriage (n=1), and structural anomalies on ultrasound (n=1). CONCLUSIONS: RAAs at NIPT might be associated with fetal uniparental disomy, mosaic aneuploidy, and poor pregnancy outcomes, but most positive cases have normal pregnancy outcomes. For RAAs, genetic counseling on the potential risks of abnormal NIPT results, as well as on benefits and limitations of invasive prenatal diagnosis, might help guide clinical management.

10.
Ann Palliat Med ; 2020 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-33183057

RESUMO

BACKGROUND: The associations between circulating tumor cells (CTCs) in peripheral blood and prognosis of patients with esophageal carcinoma (EC) have been investigated by a number of studies, but the results are not consistent. Therefore, this study aimed to explore this controversial subject. METHODS: A literature database search was performed according to preferred reporting items for systematic reviews and meta-analyses (PRISMA) statement. The risk ratio (RR), hazard ratio (HR) and their 95% confidence intervals (CIs) were retained as the effect measures. If necessary, subgroup analyses and metaregression should also be performed to clarify the heterogeneity. RESULTS: Thirty-three studies, containing 3,236 patients with EC, were included in this meta-analysis. The results showed that overall survival (OS) (HR =2.14; 95% CI, 1.73-2.65) and progression-free survival (PFS) (HR =2.29; 95% CI, 1.69-3.11) were worse in CTCs-positive patients. CTC positivity is also significantly associated with depth of infiltration (RR =1.42; 95% CI, 1.10-1.82, P=0.21) and tumor-node-metastasis (TNM) stage (RR =1.36; 95% CI, 1.09-1.69, P=0.22). However, there was no significant relationship between CTC-positive and distant metastasis (RR =1.58; 95% CI, 1.00-2.50, P=0.65). CONCLUSIONS: Detection of CTCs had prognostic value for EC patients. Positive CTC is associated with poor prognosis and some prognostic factors, such as depth of infiltration and TNM stage, but not related to metastasis.

11.
Zhongguo Dang Dai Er Ke Za Zhi ; 22(11): 1209-1214, 2020 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-33172557

RESUMO

OBJECTIVE: To observe the incidence of malnutrition and nutritional risk in children with pneumonia on mechanical ventilation in the pediatric intensive care unit (PICU), and to explore the nutritional support effect of short-peptide enteral nutrition formula. METHODS: A total of 68 children with severe pneumonia who were hospitalized in the PICU from October 2017 to October 2018 and required mechanical ventilation were enrolled for a prospective randomized controlled study. The children were randomly divided into a control group and an experimental group. Through the nasogastric feeding tube, the experimental group received the short-peptide enteral nutrition formula, and the control group received the intact-protein enteral nutrition formula. The weight-for-age Z score, STRONGkids nutritional risk score, and pediatric critical illness score of the two groups were evaluated. The serum levels of total protein, albumin, and prealbumin (PA) on admission and before discharge were measured. The gastrointestinal tolerance and clinical outcome indicators of the two groups were observed. RESULTS: Among the 68 mechanically ventilated children, 26 (38%) had malnutrition, including moderate malnutrition (10 cases, 15%) and severe malnutrition (16 cases, 24%); 10 cases (15%) had malnutrition at discharge. Sixty-three children (93%) had nutritional risk, including moderate nutritional risk in 21 cases and high nutritional risk in 42 cases. The moderate and high nutritional risk rates of the critical and extreme critical groups were significantly higher than those of the non-critical group (P<0.05). Compared with the control group, the experimental group had significantly shorter duration of mechanical ventilation and total length of hospital stay, significantly higher serum PA level and weight growth rate, and significantly better gastrointestinal tolerance (P<0.05). There were no significant differences in the incidence of ventilator-associated pneumonia and disease outcome between the two groups (P>0.05). CONCLUSIONS: The detection rates of malnutrition and nutritional risk in children with pneumonia on mechanical ventilation are relatively high. Short-peptide enteral nutrition formula can help improve their treatment outcome and are more suitable for nutritional support in critically ill children on mechanical ventilation.

12.
Rev Esp Enferm Dig ; 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33207885

RESUMO

OBJECTIVE: To assess the efficacy and safety of endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) in the treatment of colorectal laterally spreading tumors (LSTs). METHODS: A systematic literature search was performed in Pubmed, Embase, the Cochrane Library, CNKI and WANFANG databases. The related literatures were selected according to certain inclusion and exclusion criteria. The Cochrane Collaboration's Revman 5.3 software was used for data analysis. RESULTS: A total of 12 studies were included in the analysis. The total number of lesions was 3062 (EMR: 1906; ESD: 1156). The en bloc resection rate of ESD was 95% (1098/1156), which was significantly higher than that of EMR (42.8%, 815/1906) [OR=0.07, 95% CI (0.02, 0.07), P<0.00001]. The complete resection rate of ESD was 93.2% (109/117), which was significantly higher than that of EMR as well (71.9%, 92/128) [OR=0.12,95% CI (0.05, 0.29), P<0.00001]. The bleeding rate showed no significant difference between EMR and ESD (4.2% vs. 3.5%) [OR=1.04, 95% CI (0.68, 1.60), P=0.85]. The perforation rates of EMR and ESD were 1.8% and 2.4%, respectively, which displayed significant difference [OR=0.56, 95% CI (0.32,0.97), P=0.04]. Nevertheless, the recurrence rate of EMR was significantly higher than that of ESD (15.9% vs. 0.5%) [OR=23.06, 95% CI (11.11, 47.85), P<0.00001]. CONCLUSIONS: Endoscopic resection of LSTs is safe and effective. As compared with EMR, ESD has higher en-bloc and complete resection rates but a lower recurrence rate. Therefore, ESD is highly recommended for treatment of LSTs.

13.
Future Microbiol ; 15: 1343-1352, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-33085541

RESUMO

Aim: The inference of coronavirus evolution is largely based on mutations in SARS-CoV-2 genome. Misinterpretation of these mutations would mislead people about the evolution of SARS-CoV-2. Materials & methods: With 4521 lines of SARS-CoV-2, we obtained 3169 unique point mutation sites. We counted the numbers and calculated the minor allele frequency (MAF) of each mutation type. Results: Nearly half of the point mutations are C-T mismatches and 20% are A-G mismatches. The MAF of C-T and A-G mismatches is significantly higher than MAF of other mutation types. Conclusion: The excessive C-T mismatches do not resemble the random mutation profile. They are likely to be caused by the cytosine-to-uridine deamination system in hosts.


Assuntos
Betacoronavirus/genética , Mutação , RNA Viral/metabolismo , Pareamento Incorreto de Bases , Uso do Códon , Infecções por Coronavirus/virologia , Citosina/metabolismo , Bases de Dados Genéticas , Desaminação , Frequência do Gene , Genoma Viral , Humanos , Pandemias , Pneumonia Viral/virologia , Polimorfismo de Nucleotídeo Único , Uridina/metabolismo
14.
Postgrad Med J ; 2020 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-33070116

RESUMO

STUDY PURPOSE: Malignant central airway obstruction (CAO) in non-small cell lung cancer (NSCLC) is associated with high morbidity and requires endobronchial palliative treatment to re-establish a free air passage. We investigate intratumoral therapy combining anti-angiogenic and cytotoxic as a feasible therapeutic modality to treat malignant CAO. STUDY DESIGN: Ten NSCLC subjects with symptomatic malignant CAO underwent endobronchial intratumoral cisplatin and Endostar co-injection after tumour debulking next to systemic cisplatin-based chemotherapy. Injection was performed immediately after debulking surgery and was then carried out on day 2, day 6 and day 10 past systemic chemotherapy. Nine subjects of control group constantly received traditional cisplatin-based chemotherapy. Bronchoscopy, CT scanning, histology, FEV1/FVC ratio, Karnofsky performance (KPS) and shortness of breath scores were analysed to assess therapeutic efficacy. RESULTS: All 10 subjects benefited from the intratumoral cisplatin and endostar co-injection and systemic chemotherapy combination therapy. Bronchoscopy and CT scanning analyses showed a massive airway widening after treatment. Increased KPS and reduced shortness of breath score were also observed. A substantial improvement of lung function was further confirmed by increased FEV1/FVC ratio. For subjects of control group, the improvement was moderate and obviously not as optimal as the 10 subjects with intratumoral injection. CONCLUSIONS: We have shown that the intratumoral injection of cytotoxic cisplatin plus anti-angiogenic Endostar is an effective and safe adjuvant therapeutic option to treat malignant CAO in clinical practice. This time-staggered local and systemic treatment combination improves quality of life and clinical parameters, thus may provide a feasible therapeutic option for symptomatic CAO.

15.
Acta Derm Venereol ; 100(17): adv00299, 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33047146

RESUMO

Filaggrin, encoded by the FLG gene, plays a crucial role in the barrier function of epidermis, but the association between FLG loss-of-function mutations and infectious skin diseases has not been systematically studied. FLG coding sequences from 945 patients with leprosy and 916 healthy controls were captured and enriched using an array-based high-throughput system, and subjected to next-generation sequencing. The loss-of-function mutations found were further validated by Sanger sequencing. A total of 21 loss-of-function mutations were found in 945 patients with leprosy, with a carrier rate of 17.53%, while the prevalence of these mutations in 916 healthy controls was 14.77%, which was significantly lower than in patients. Two individual FLG loss-of-function mutations (K4022X and Q1790X) were found to be significantly associated with leprosy. These results suggest a possible role for filaggrin in defending against leprosy pathogens.

16.
Europace ; 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33083840

RESUMO

AIMS: To establish a simple criterion for determining a failed His-bundle pacing (HBP). This criterion states that if stimulus to QRS end interval is longer than His-bundle potential to QRS end interval ('S-QRSend > H-QRSend') then a failed HBP can be determined. METHODS AND RESULTS: We performed retrospective analysis on 737 pacing tests around His-bundle in 241 patients and prospective analysis on 400 tests in 123 patients. A successful HBP is defined as that whole His-bundle is captured with or without capture of adjacent ventricular myocardium, otherwise, a failed HBP was considered. The output criteria and effective refractory period criteria were used as the gold standards for determining a successful HBP. The gold standards are that if decreasing the pacing output or pacing cycle length to a certain level results in duration or morphology changes of QRS, then a successful HBP is ascertained. In retrospective analysis of patients with normal His-Purkinje conduction, a failed HBP was determined in 31% (154/492) of pacing tests according to 'S-QRSend > H-QRSend'; all of them were validated by the gold standards (specificity = 100%). In prospective study, a failed HBP was confirmed according to the simple criterion with 100% accuracy in 33% (79/241) pacing tests. This simple criterion was also suitable for patients with His-Purkinje conduction disease although cases with 'S-QRSend > H-QRSend' rarely occurred. CONCLUSION: A failed HBP can be easily and reliably determined solely by 'S-QRSend > H-QRSend' in more than 30% pacing tests.

17.
Health Qual Life Outcomes ; 18(1): 339, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33046101

RESUMO

PURPOSE: To explore the association of academic performance and general health status with health-related quality of life (HRQoL) in school-aged children and adolescents in China. METHODS: In this cross-sectional study conducted in 2018, students (grade 4-12) were randomly chosen from primary and high schools in Nanjing, China. HRQoL, the outcome measure, was recorded using the Child Health Utility 9D, while self-rated academic performance and general health were the independent variables. Mixed-effects regression models were applied to compute mean difference (MD) and 95% confidence interval (CI) of HRQoL utility score between students with different levels of academic performance and general health. RESULTS: Totally, 4388 participants completed the study, with a response rate of 97.6%. The mean HRQoL utility score was 0.78 (SD: 0.17). After adjustment for socio-demographic attributes, physical activity, sedentary behavior, dietary patterns, body weight status and class-level clustering effects, students with fair (MD = 0.048, 95% CI 0.019, 0.078) and good (MD = 0.082, 95% CI 0.053, 0.112) self-rated academic performance reported higher HRQoL utility scores than those with poor academic performance, respectively. Meanwhile, students with fair (MD = 0.119, 95% CI 0.083, 0.154) and good (MD = 0.183, 95% CI 0.148, 0.218) self-assessed general health also recorded higher HRQoL utility scores than those with poor health, separately. Consistent findings were observed for participants by gender, school type and residential location. CONCLUSIONS: Both self-rated academic performance and general health status were positively associated with HRQoL among Chinese students, and such relationships were independent of lifestyle-related behaviors and body weight status.

18.
World J Gastroenterol ; 26(37): 5682-5692, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33088161

RESUMO

BACKGROUND: The significance of Helicobacter pylori (H. pylori) infection and atrophic gastritis (AG) in the prevalence of colorectal adenomas has been examined in a limited number of studies. However, these studies reported disputed conclusions. AIM: To investigate whether H. pylori infection, AG, and H. pylori-related AG increase the risk of colorectal adenomas. METHODS: This retrospective cross-sectional study included 6018 health-check individuals. The relevant data for physical examination, laboratory testing, 13C-urea breath testing, gastroscopy, colonoscopy and histopathological examination of gastric and colorectal biopsies were recorded. Univariate and multivariate logistic regression analyses were performed to determine the association between H. pylori-related AG and colorectal adenomas. RESULTS: Overall, 1012 subjects (16.8%) were diagnosed with colorectal adenomas, of whom 143 (2.4%) had advanced adenomas. Among the enrolled patients, the prevalence of H. pylori infection and AG was observed as 49.5% (2981/6018) and 10.0% (602/6018), respectively. Subjects with H. pylori infection had an elevated risk of colorectal adenomas (adjusted odds ratio [OR] of 1.220, 95% confidence interval (CI): 1.053-1.413, P = 0.008) but no increased risk of advance adenomas (adjusted OR = 1.303, 95%CI: 0.922-1.842, P = 0.134). AG was significantly correlated to an increased risk of colorectal adenomas (unadjusted OR = 1.668, 95%CI: 1.352-2.059, P < 0.001; adjusted OR = 1.237, 95%CI: 0.988-1.549, P = 0.064). H. pylori infection accompanied by AG was significantly associated with an increased risk of adenomas (adjusted OR = 1.491, 95%CI: 1.103-2.015, P = 0.009) and advanced adenomas (adjusted OR = 1.910, 95%CI: 1.022-3.572, P = 0.043). CONCLUSION: H. pylori-related AG was associated with a high risk of colorectal adenomas and advanced adenomas in Chinese individuals.

19.
Adv Ther ; 2020 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-33094477

RESUMO

INTRODUCTION: To investigate the risk factors affecting the prognosis of elderly patients with severe aneurysmal subarachnoid hemorrhage (aSAH). METHODS: Forty-nine elderly patients with severe aSAH (Hunt and Hess [H-H] grade III-V) were enrolled in this retrospective study. Follow-up was conducted with patients 30 days after discharge using the Glasgow Outcome Scale (GOS), on which scores of 1-3 indicated a poor outcome (n = 32) and scores of 4-5 indicated a good outcome (n = 17). The patients' general information (gender, age, presence of hypertension, diabetes, or coronary atherosclerotic heart disease, location of ruptured aneurysm, and H-H grade) and complications (cerebral vasospasm, new cerebral infarction, pulmonary infection, liver dysfunction, hypoalbuminemia, anemia, and electrolyte disturbance) were recorded, and comparison between the different outcome groups was undertaken. Univariate analysis was used to analyze the factors associated with different outcomes, and multivariate logistic regression analysis was used to determine the factors that lead to poor outcomes. RESULTS: The incidence of all complications increased in patients with higher H-H grades, but without statistical significance (P > 0.05). There was no statistically significant difference between the two outcome (poor and good) groups in general information and complications (all P > 0.05), with the exception of different H-H grades (P < 0.05). H-H grade was a statistically significant risk factor for poor outcomes in elderly patients with severe aSAH (OR 11.627, 95% CI 2.475-55.556, P = 0.002). CONCLUSION: H-H grade is an independent factor related to the prognosis of elderly patients with severe aSAH.

20.
Asian Pac J Cancer Prev ; 21(10): 2987-2992, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33112558

RESUMO

OBJECTIVE: Poly (ADP-ribose) polymerase 1 (PARP1), as a key enzyme in the base excision repair pathway, plays a crucial role in tumorigenesis and progression. This study aimed to assess whether polymorphisms of PARP1 gene could be used as predictive biomarkers for the survival of esophageal squamous cell carcinoma (ESCC) patients from Cixian high-incidence region in northern China. METHODS: In 203 ESCC patients with survival information, PARP1 rs1136410 T/C and rs8679 T/C single nucleotide polymorphisms (SNPs) were genotyped by polymerase chain reaction ligase detection reaction (PCR-LDR) method. All statistical analyses were performed using the SPSS ver. 22.0 software package (SPSS, Chicago, IL, USA). RESULTS: The mean age ± standard deviation of the ESCC patients was 60.4 ± 7.9 years. There was no significant relation of sex, age, smoking status and upper gastrointestinal cancer family history with the survival time of the ESCC patients. The mean survival time of rs1136410 T/T, T/C and C/C genotype carriers were 43.3, 42.3 and 46.6 months, respectively. The rs1136410 was not associated with the survival time of the ESCC patients. For rs8679, the mean survival time of T/T genotype carriers was 43.7 months, which was not significantly different from that of the patients with T/C genotype (42.1 months). CONCLUSION: In Cixian high-incidence region from northern China, rs1136410 and rs8679 SNPs might not be used to predict survival of ESCC patients. There is a need to explore whether other SNPs of PARP1 gene have an effect on prognosis of ESCC patients.

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