A visible BODIPY-based sensor for 'Naked-Eye' recognition of Ag+ and its application on test paper strips.

In this study, a novel, highly sensitive fluorescent sensor (E)-2-((2-(benzo[d] thiazol-2-yl) quinolin-8-yl) oxy)-N'-(4-(5, 5-difluoro-1, 3, 7, 9-tetramethyl-5H-4λ4, 5λ4-dipyrrolo [1, 2-c:2', 1'-f] [1, 3, 2] diazaborinin-10-yl) benzylidene) acetohydrazide (TQB) was developed for dual mode of Ag+ detection (colorimetric/fluorescence), and its structural and spectral properties were characterized by 1H NMR, ESI-MS, X-ray, ultraviolet and fluorescence photometry. It is found that TQB could specifically and efficiently identify Ag+ among many other metal ions in CH3OH/H2O (7:3 v/v, pH = 7.23) buffer. The maximum absorption wavelength of TQB is red-shifted while its fluorescence is quenched with a quenching rate of 88.7%. The energy difference between TQB and TQB-Ag+ complex was calculated by DFT, and the applicability of TQB was verified by paper strip test. In addition, TQB has been successfully applied to the determination of Ag+ in real water samples with good reversibility and recoveries.

Quinoa protein/polysaccharide electrostatic complex stabilized vegan high internal phase emulsions for 3D printing: Role of complex state and gelling-type polysaccharides.

Rational selection of the complex state and polysaccharide type may enhance the performance of electrostatic complex stabilized high internal phase emulsions (HIPEs). Herein, quinoa proteins were extracted to form electrostatic complexes separately with three gelling-type polysaccharides to fabricate HIPEs. Results showed that the complexes in soluble state (pH 8.4-5.6) exhibited moderate size, high negative potential and enhanced protein hydrophobicity, and could achieve HIPEs with 84% oil phase upon acidification to pH 6 at low concentrations. Its excellent interfacial structure enhanced stability during heating, freeze-thawing and long-term storage, and exhibited promising 3D printing potential. Furthermore, the complexes formed by sulfated polysaccharide carrageenan had higher amphiphilicity than those formed by carboxylated polysaccharide pectin or sodium alginate, and their stabilized HIPE had preferable droplet size, stability and 3D printing resolution than its counterparts. This study may provide new insights into the performance enhancement of protein/polysaccharide electrostatic complex stabilized HIPEs.

Dialdehyde starch-enclosed silver nanoparticles substrate with controlled-release "hotspots" for ultrasensitive SERS detection of thiabendazole.

Pesticide residues have long been a major concern for food safety. In this study, a dialdehyde starch-encapsulated silver nanoparticles composite with controlled-release "hotspots" was developed as a surface-enhanced Raman scattering (SERS) substrate. At room temperature, most of the Ag NPs were encapsulated in dialdehyde starch, which is beneficial for improving stability, and when heated to the gelatinization point, Ag NPs are completely released and abundant hot spots are formed. We demonstrated sensitive detection of thiabendazole (TBZ) in or on the surface of an apple by means of two ways, i.e., detecting the analyte in solution after pretreatment and in-situ detecting the analyte by using a flexible paper-based substrate. The results showed that the detection limits of TBZ by the two ways were 0.052 ppm and 0.051 ppm respectively, and the recoveries of TBZ range from 96.80 % to 105.46 %. Overall, this SERS substrate shows great potential for pesticide residue detection in food.

Impact of short-term ambient air pollution exposure on the risk of severe COVID-19.

Ecological studies suggested a link between air pollution and severe COVID-19 outcomes, while studies accounting for individual-level characteristics are limited. In the present study, we aimed to investigate the impact of short-term ambient air pollution exposure on disease severity among a cohort of 569 laboratory confirmed COVID-19 patients admitted to designated hospitals in Zhejiang province, China, from January 17 to March 3, 2020, and elucidate the possible biological processes involved using transcriptomics. Compared with mild cases, severe cases had higher proportion of medical conditions as well as unfavorable results in most of the laboratory tests, and manifested higher air pollution exposure levels. Higher exposure to air pollutants was associated with increased risk of severe COVID-19 with odds ratio (OR) of 1.89 (95% confidence interval (CI): 1.01, 3.53), 2.35 (95% CI: 1.20, 4.61), 2.87 (95% CI: 1.68, 4.91), and 2.01 (95% CI: 1.10, 3.69) for PM2.5, PM10, NO2 and CO, respectively. OR for NO2 remained significant in two-pollutant models after adjusting for other pollutants. Transcriptional analysis showed 884 differentially expressed genes which mainly were enriched in virus clearance related biological processes between patients with high and low NO2 exposure levels, indicating that compromised immune response might be a potential underlying mechanistic pathway. These findings highlight the impact of short-term air pollution exposure, particularly for NO2, on COVID-19 severity, and emphasize the significance in mitigating the COVID-19 burden of commitments to improve air quality.

Microplastic pollution in terrestrial ecosystems: Global implications and sustainable solutions.

Microplastic (MPs) pollution has become a global environmental concern with significant impacts on ecosystems and human health. Although MPs have been widely detected in aquatic environments, their presence in terrestrial ecosystems remains largely unexplored. This review examines the multifaceted issues of MPs pollution in terrestrial ecosystem, covering various aspects from additives in plastics to global legislation and sustainable solutions. The study explores the widespread distribution of MPs worldwide and their potential antagonistic interactions with co-occurring contaminants, emphasizing the need for a holistic understanding of their environmental implications. The influence of MPs on soil and plants is discussed, shedding light on the potential consequences for terrestrial ecosystems and agricultural productivity. The aging mechanisms of MPs, including photo and thermal aging, are elucidated, along with the factors influencing their aging process. Furthermore, the review provides an overview of global legislation addressing plastic waste, including bans on specific plastic items and levies on single-use plastics. Sustainable solutions for MPs pollution are proposed, encompassing upstream approaches such as bioplastics, improved waste management practices, and wastewater treatment technologies, as well as downstream methods like physical and biological removal of MPs. The importance of international collaboration, comprehensive legislation, and global agreements is underscored as crucial in tackling this pervasive environmental challenge. This review may serve as a valuable resource for researchers, policymakers, and stakeholders, providing a comprehensive assessment of the environmental impact and potential risks associated with MPs.

Visualization of conformational transition of GRP94 in solution.

GRP94, an ER paralog of the heat-shock protein 90 family, binds and hydrolyses ATP to chaperone the folding and maturation of its selected clients. Compared with other hsp90 proteins, the in-solution conformational dynamics of GRP94 along the ATP hydrolysis cycle are less understood, hindering our understanding of its chaperoning mechanism. Leveraging small-angle X-ray scattering, negative-staining EM, and hydrogen-deuterium exchange coupled mass-spec, here we show that in its apo form, ∼60% of mouse GRP94 (mGRP94) populates an "extended" conformation, whereas the rest exist in either "close V" or "twist V" like "compact" conformations. Different from other hsp90 proteins, the presence of AMPPNP only impacts the relative abundance of the two compact conformations, rather than shifting the equilibrium between the "extended" and "compact" conformations of mGRP94. HDX-MS study of apo, AMPPNP-bound, and ADP-bound mGRP94 suggests a conformational transition from "twist V" to "close V" upon ATP binding and a back transition from "close V" to "twist V" upon ATP hydrolysis. These results illustrate the dissimilarities of GRP94 in conformation transition during ATP hydrolysis from other hsp90 paralogs.

Gestational GenX and PFOA exposures induce hepatotoxicity, metabolic pathway, and microbiome shifts in weanling mice.

Ammonium perfluoro (2-methyl-3-oxahexanoate) (GenX), a replacement for perfluorooctanoic acid (PFOA), has been detected in multiple environmental media and biological samples worldwide. Accumulated evidence implies that GenX exposure might exert adverse health effects, although the underlying mechanisms have not been fully revealed. In this study, pregnant BALB/c mice were exposed to GenX (2 mg/kg/day), PFOA (1 mg/kg/day), or Milli-Q water by gavage from the first day of gestation (GD0) until GD21. Necropsy and tissue collection were conducted in pups at 4 weeks of age. PFOA and GenX induced similar histopathological changes in both the liver and the intestinal mucosa, accompanied by higher serum levels of alanine and aspartate aminotransferase. Moreover, the capacity of hepatic glycogen storage and intestinal mucus secretion were significantly decreased, suggesting dysfunction of liver metabolism and the intestinal mucosal barrier. A total of 637 and 352 differentially expressed genes (DEGs) were identified in the liver tissues of GenX and PFOA group, respectively. Most of the enriched pathways from the DEGs by KEGG enrichment analysis were metabolism-associated. Moreover, overexpression of CYP4A14, Sult2a1, Cpt1b, Acaa1b, Igfbp1, Irs-2 and decreased expression of Gys2 were observed in livers of GenX exposed pups, supporting the hypothesis that there was metabolic disruption. Furthermore, DNA damage and cell cycle arrest proteins (Gadd45ß, p21, Ppard) were significantly increased, while cell proliferation-related proteins (Cyclin E, Myc, EGFR) were decreased by gestational GenX exposure in the pups' liver. In addition, imbalance of gut microbiota and dysfunction of the intestinal mucosa barrier might contribute to hepatotoxicity at least in part. Taken together, our results suggested that gestational GenX exposure triggered metabolic disorder, which might be responsible for the hepatotoxicity in the pups in addition to dysfunction of the intestinal mucosa barrier. This study enriches the mechanisms of GenX-induced developmental hepatotoxicity by associating metabolic disorder with intestinal homeostasis.

Whole-body aging mediates the association between exposure to volatile organic compounds and osteoarthritis among U.S. middle-to-old-aged adults.

BACKGROUND: Humans are constantly exposed to various volatile organic compounds (VOCs) because of their widespread sources and characteristic of easy evaporation. Existing evidence regarding the association between VOC exposure and osteoarthritis (OA) risk is limited. PURPOSE: This study aimed to investigate the associations between individual urinary VOC metabolites (VOCMs) and the VOCM mixture, representing internal exposure levels of VOCs, with prevalent OA risk and to explore the mediating effect of aging and oxidative stress (OS) in these associations. METHODS: Data from the National Health and Nutrition Examination Surveys 2005-2020 were analyzed. Weighted generalized linear regression was employed to explore the associations between individual VOCMs and OA risk, as well as aging and OS biomarkers. A five-repeated ten-fold cross-validation elastic net model was used to identify critical VOCMs for the weight quantile sum (WQS) analysis, which was performed to explore the VOCM mixture and OA risk association. Parallel and serial mediation analyses were conducted to identify the potential mediators and mediation pathways. RESULTS: This study included 6578 American adults aged ≥40 years, among whom 1052 (16.0 %) individuals reported prevalent OA. Urinary levels of N-acetyl-S-(benzyl)-L-cysteine, mandelic acid and phenylglyoxylic acid were positively associated with OA risk. Eleven VOCMs with nonzero coefficients were identified and included in the WQS analysis, and results revealed an average increase of 24.4 % in OA risk (OR = 1.244, 95 % CI: 1.041, 1.486) per one-quantile increment in the VOCM mixture. Two aging biomarkers, phenotypic age and biological age, parallelly mediated the association between the VOCM mixture and OA risk, with mediation effect proportions of 9.0 % and 16.4 %, respectively. CONCLUSIONS: Exposure to VOCs is associated with an increased OA risk in middle-to-old aged American adults. The mediating effect of aging contributes to the association between co-exposure to VOCs and OA risk. Further prospective studies are required to substantiate these findings.

Environmental fate, aging, toxicity and potential remediation strategies of microplastics in soil environment: Current progress and future perspectives.

Microplastics (MPs) are small plastic debris (<5 mm) that result from the fragmentation of plastic due to physical and physiochemical processes. MPs are emerging pollutants that pose a significant threat to the environment and human health, primarily due to their pervasive presence and potential bioaccumulation within the food web. Despite their importance, there is a lack of comprehensive studies on the fate, toxicity, and aging behavior of MPs. Therefore, this review aims to address this gap by providing a cohesive understanding of several key aspects. Firstly, it summarizes the sources and fate of MPs, highlighting their ubiquitous presence and the potential pathways through which they enter ecosystems. Secondly, it evaluates the aging process of MPs and the factors influencing it, including the morphological and physiological changes observed in crops and the release of pollutants from aged MPs, which can have detrimental effects on the environment and human health. Furthermore, the impacts of aging MPs on various processes are discussed, such as the mobilization of other pollutants in the environment. The influence of aged MPs on the soil environment, particularly their effect on heavy metal adsorption, is examined. Finally, the review explores strategies for the prevention technologies and remediation of MPs, highlighting the importance of developing effective approaches to tackle this issue. Overall, this review aims to contribute to our understanding of MPs, their aging process, and their impacts on the environment and human health. It underscores the urgency of addressing the issue of MPs and promoting research and remediation efforts to mitigate their adverse effects.

SIRT1 inhibitors within Qing-Luo-Yin alleviated white adipose tissues-mediated inflammation in antigen-induced arthritis mice.

BACKGROUND: White adipose tissues (WAT) release large amounts of inflammatory mediators, which are responsible for the pathology of rheumatoid arthritis (RA). PURPOSE: The current study investigated the involvement of WAT in the treatments of antigen-induced arthritis (AIA) mice with the herbal formula Qing-Luo-Yin (QLY). METHODS: Cytokines and biochemical/metabolic indicators were determined by ELISA and colorimetry methods, respectively. Monocytes were analyzed by flow cytometry. Tissues were subjected to PCR, western-blot and histological analyses. Pre-adipocytes were cultured in the different mouse serum from the in vivo experiment, and some of them were treated by certain compounds or/and lipopolysaccharide. Afterwards, the catalytic activity and thermostability of SIRT1 were tested. Gene/protein expression and cytokine production were investigated too. NAMPT and SIRT1 were silenced in some cells by siRNA. RESULTS: AIA mice suffered from inflammatory adipokines-mediated metabolism and immune disorders. Besides joint protective effects, QLY therapies favored adipocyte differentiation and suppressed inflammatory adipokines release. The up-regulation of fatty acid oxidation and inflammatory monocyte polarization was therefore inhibited in peripheral tissues. PPARγ expression was generally promoted by QLY. Whereas, SIRT1 activity was always impaired, indicated by the declined NAD+ levels and the increased ace-p65 expression. QLY effectively inhibited eNAMPT release in AIA mouse serum-cultured pre-adipocytes. This effect was antagonized by resveratrol (a SIRT1 agonist) and overshadowed by NAMPT silencing. QLY-related compounds berberine, dioscin and sophocarpine showed high binding affinities to SIRT1, stabilized this protein, and inhibited its deacetylation activity in vitro. Their effects on ace-p65 expression were weakened when SIRT1 was silenced. CONCLUSION: SIRT1 inhibitors in QLY reduced eNAMPT production and up-regulated PPARγ in AIA mice, leading to inflammation remission. These clues show that except for the well-known anti-inflammatory functions, SIRT1 participates in inflammatory reactions too and could be a potential anti-rheumatic target.

A novel cross-disciplinary approach for estimating spatio-temporal pattern and drivers of pollution transfer caused by industrial transfer processes.

Food security is the basic requirement for human health and social stability. However, food pollution risk caused by economic development models, e.g., industrial transfer, is poorly understood. In this study, we developed and tested a point-area-domain cross-disciplinary approach to quantitatively estimate patterns and processes of soil and rice heavy metal (Pb, Hg and Cd) pollution risk caused by the fourth global industrial transfer process in CG Province of China. Our results indicate that the industrial transfer policy caused obvious cross-regional soil Pb, Hg and Cd pollution and industrial transfer. Industrial transfer process largely exacerbated rice Hg pollution risk in industrial transfer undertaking areas, mainly in the west (29.18%), periphery (28.38%) and south (25.88%) regions. The concentration increase in the west ranged from 0.0001 to 0.0775 mg/kg (mean±S.D. 0.00327 ± 0.00447, median 0.0026 mg/kg). The most important industries regarding rice Hg pollution in industrial transfer undertaking areas included special equipment (26.53%)>metal processing (11.93%)>communication equipment (11.25%)>chemical product (10.90%)> electrical machinery and equipment (9.59%). The pollution transfer was mainly attributed to environmental regulations, factor endowment and industrial structure. Our study highlights the importance for the integration of industrial transfer into food security and poverty reduction plans in the developing regions. ENVIRONMENTAL IMPLICATION: Food security is the basic requirement for human health and social stability. However, food pollution risk caused by economic development models, e.g., industrial transfer, is poorly understood. We developed and tested a point-area-domain cross-disciplinary approach to quantitatively estimate patterns and processes of soil and rice heavy metal pollution risk caused by the fourth global industrial transfer process. Our results suggest that industrial transfer process largely exacerbated soil and rice Hg pollution risk in the southeast coastal areas of China. Our study highlights the importance for the integration of industrial transfer into food security strategies in developing countries.

Developing a novel benzothiazole-based red-emitting probe for intravital imaging of superoxide anion.

Superoxide anion (O2•-), the first generated reactive oxygen species (ROS), is a critical player in cellular signaling network and redox homeostasis. Imaging of O2•-, particularly in vivo, is of concern for further understanding its roles in pathophysiological and pharmacological events. Herein, we designed a novel probe, (E)-4-(5-(2-(benzo[d]thiazol-2-yl)-2-cyanovinyl)furan-2-yl)phenyl trifluoromethane-sulfonate (BFTF), by modifying hydroxyphenyl benzothiazole (a widely used dye scaffold) which includes insertion of both an acrylonitrile unit and a furan ring to extend the total π-conjugation system and to enhance push-pull intramolecular charge transfer process, and utilization of trifluoromethanesulfonate as the response unit. Toward O2•-, the probe features near-infrared fluorescent emission (685 nm), large Stokes shift (135 nm), and deep tissue penetration (300 µm). With its help, we successfully mapped preferential generation of O2•- in HepG2 cells over L02 cells, as well as in A549 over BEAS-2B cells by ß-lapachone (an anticancer agent that generates O2•-), and more importantly, visualized overproduction of O2•- in living mice with liver injury induced by acetaminophen (a well-known analgesic and antipyretic drug).

Assessing stress responses in potherb mustard (Brassica juncea var. multiceps) exposed to a synergy of microplastics and cadmium: Insights from physiology, oxidative damage, and metabolomics.

Both microplastics (MPs) and cadmium (Cd) are common contaminants in farmland systems, is crucial for assessing their risks for human health and environment, and little research has focused on stress responses mechanisms of crops exposed to the combined pollution. The present study investigated the impact of polyethylene (PE) and polypropylene (PP) microplastics (MPs), in combination with Cd, on the physiological and metabolomic changes as well as rhizosphere soil of potherb mustard. Elevated levels of PEMPs and PPMPs were found to impede nutrient uptake in plants while promoting premature flowering, and the concomitant effect is lower crop yields. The substantial improvement in Cd bioavailability facilitated by MPs in rhizosphere soil, especially in high concentrations of MPs, then elevated bioavailability of Cd contributed to promoted Cd accumulation in plants, with distinct effects depending on the type and concentration of MPs. The presence of MPs Combined exposure to high concentrations of MPs and Cd resulted in alterations in plant physiology and metabolomics, including decreased biomass and photosynthetic parameters, elevated levels of reactive oxygen species primarily H2O2, increased antioxidant enzyme activities, and modifications in metabolite profiles. Overall, our study assessed the potential impact on food security (the availability of cadmium to plant) and crops stress responses regarding the contamination of MPs and Cd, providing new insights for future risk assessment in agriculture.

Integrating metabolomics and high-throughput sequencing to investigate the effects of tire wear particles on mung bean plants and soil microbial communities.

Tire wear particles (TWPs) generated by vehicle tires are ubiquitous in soil ecosystems, while their impact on soil biota remains poorly understood. In this study, we investigated the effects of TWPs (0.1%, 0.7%, and 1.5% of dry soil weight) on the growth and metabolism of mung bean (Vigna radiata) plants over 32 days in soil pots. We found that TWPs-treated soils had high levels of heavy metals and polycyclic aromatic hydrocarbons (PAHs). However, there was no significant impact of TWPs exposure on plant growth, suggesting that mung bean plants have a degree of tolerance to TWPs. Despite the lack of impact on plant growth, exposure to TWPs had significant effects on soil enzyme activities, with a decrease of over 50% in urease and dehydrogenase activity. Furthermore, TWPs exposure resulted in marked changes in the plant metabolite profile, including altered levels of sugars, carboxylic acids, and amino acids, indicating altered nitrogen and amino acid-related metabolic pathways. TWPs exposure also disrupted the rhizospheric and bulk soil microbiota, with a decrease in the abundance of bacterial (Blastococcus) and fungal (Chaetomium) genera involved in nitrogen cycles and suppressing plant diseases. In summary, our study provides new insights into the effects of TWPs on plants and soil, highlighting the potential ecological consequences of TWPs pollution in terrestrial ecosystems and underscoring the need for further research in this area.

The neuroprotective mechanisms of naringenin: Inhibition of apoptosis through the PI3K/AKT pathway after hypoxic-ischemic brain damage.

ETHNOPHARMACOLOGICAL RELEVANCE: Naringenin (NGN) is a widely distributed flavonoid with potent antioxidant and neuroprotective properties. Neuroprotective agents play a crucial role in the treatment of hypoxic-ischemic encephalopathy (HIE). It has shown potential therapeutic effects for neurological disorders. However, its efficacy on HIE is yet to be investigated. AIM OF THE STUDY: This study aims to investigate the potential neuroprotective effect of naringenin and its underlying molecular mechanisms in reducing oxidative stress, apoptosis, and improving brain outcomes following HIE. Additionally, the study aims to identify the potential targets, mechanisms, and functions of naringenin using network pharmacology analysis. MATERIALS AND METHODS: Neonatal mice were exposed to the hypoxic-ischemic brain damage (HIBD) model to determine brain water content, and brain tissue was subjected to hematoxylin and eosin (HE), immunohistochemistry (IHC), terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and Nissl staining to investigate its neuroprotective effects. Furthermore, the neonatal mouse primary neuron oxygen-glucose deprivation (OGD) model to measure reactive oxygen species (ROS) production in vitro. The protein levels were characterized by Western Blot, and mRNA levels were evaluated by a real-time quantitative PCR detecting system (qPCR). Transmission electron microscopy (TEM) and mitochondrial fluorescent staining were used to observe mitochondrial morphology. Neuronal nuclei (NeuN) and microtubule-associated protein 2 (MAP2) were detected by Immunofluorescence (IF). Finally, network pharmacology was employed to determine the common target of naringenin and HIE. The core genes were obtained via protein-protein interaction networks (PPI) analysis and molecular docking was examined, and the mechanism of action was explored through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Additionally, small interfering RNA (siRNA) was constructed for verification. RESULTS: Naringenin has a neuroprotective effect in HIBD by modulating Vegfa expression and activating the PI3K/AKT pathway to inhibit apoptosis. Furthermore, molecular docking results suggest that Vegfa is a potential binding target of naringenin, and silencing Vegfa partially reverses the pharmacological effects of NGN. CONCLUSION: Our findings suggest that naringenin demonstrates potential clinical application for treating HIE as a novel neuroprotective agent.

Gallic acid-functionalized soy protein-based multiple cross-linked hydrogel: Mechanism analysis, physicochemical properties, and digestive characteristics.

Herein, carbodiimide hydrochloride/N-hydroxysuccinimide was used to mediate the grafting of gallic acid (GA) (0.005, 0.0015, and 0.025 wt%) with soybean protein isolate (SPI) in the preparation of SPI-GA conjugates and hydrogels. The modified materials were primarily joined via the CN bonds and exhibited excellent antioxidant properties. In addition, spectral analysis revealed that the grafting of GA increased the flexibility of the SPI structure. The SPI-GA hydrogel is fabricated through covalent/non-covalent cross-linking mechanisms, including Schiff base, Michael addition, and hydrogen bonding. Furthermore, the microstructure, rheological properties, thermal stability, and textural properties of the hydrogel were affected by the amount of GA grafted. The SPI-GA hydrogel exhibited the best performance when the amount of GA graft was 0.015 wt%. Furthermore, the tightly cross-linked structure of SPI-GA prevented premature degradation of the protein by pepsin. In conclusion, these capabilities provide numerous possibilities for the development of multifunctional and active substance delivery carriers.

Effect of polysaccharide addition on food physical properties: A review.

The texture, flavor, performance and nutrition of foods are affected by their physical properties during processing, cooking, storage, and shelf life. In addition to chemical, physical, and enzymatic modification methods, polysaccharide addition is also considered a safe, effective, and convenient food modification strategy. However, thus far, literature review on the effects of polysaccharides on the physical properties of foods is few. Therefore, the present work reviews the effects of polysaccharides on water retention capacity, rheological property, suspension ability, viscoelasticity, emulsifying property, gelling property, stability, and starch regeneration and digestion. Furthermore, the existing problems and future recommendations during food physical property modification by polysaccharides are presented. This work aims to provide some theoretical references for future research, development, and application of polysaccharides on food physical property modification.

Oxidized dextran improves the stability and effectively controls the release of curcumin loaded in soybean protein nanocomplexes.

Dextran dialdehyde (ODex) was added to a nanocomplex of soy protein isolate (SPI)-curcumin (Cur) to improve its stability and achieve controlled release of Cur. The SPI-to-ODex mass ratio was optimized to achieve excellent properties and stability. Interactions between various components were confirmed by spectroscopic analysis, and the effect of ODex on the stability and bioactivity of SPI-Cur colloids was discussed. ODex was found to be crosslinked with SPI via the Schiff base reaction, which increased the ζ-potential and improved the surface hydrophobicity of nanocomplexes. At a SPI-to-ODex mass ratio of 20:1, the nanocomplex had a smaller particle size (199.2 nm), higher ζ-potential (-45.48 mV), and higher encapsulation efficiency (96.25%). Furthermore, adding ODex changed the network structure and effectively improved the thermal and storage stability of Cur as well as its antioxidant properties. Moreover, controlled release of Cur was observed during simulated digestion in the gastrointestinal environment.

Rapid and accurate determination methods based on data fusion of laser-induced breakdown spectra and near-infrared spectra for main elemental contents in compound fertilizers.

Compound fertilizer occupies a dominant position in the structure of fertilizer products in China. The contents of nitrogen, phosphorus and potassium are the key indicators affecting the fertilization efficiency and the price of compound fertilizers. Laser-induced breakdown spectroscopy (LIBS) and near-infrared spectroscopy (NIRS) are two rapid analytical techniques suitable for online monitoring of the above components in compound fertilizer. However, accurate LIBS analysis needs to overcome matrix effects and interference from environmental elements, and NIRS also has the limitation of not being able to directly detect inorganic components in compound fertilizers. The combination of LIBS and NIRS techniques, namely LIBS-NIRS data fusion, has the potential to reduce interferences in the detection of single spectroscopic techniques and further improve the analysis accuracy. This study compared the LIBS-NIRS data fusion methods under different optimization conditions, and found that CARS-OPF (competitive adaptive reweighted sampling combined with outer product fusion) and CARS-EWF (competitive adaptive reweighted sampling combined with equal weight fusion) are two effective intermediate data fusion methods which can achieve better quantitative analysis results than single spectroscopic methods. The root mean square errors of prediction (RMSEP) for nitrogen, phosphorus, and potassium contents in compound fertilizers by using CARS-OPF are 0.901, 0.693, and 1.52, respectively, and the RMSEP for those indicators by using CARS-EWF are 0.934, 0.719, and 1.60, respectively. In these two methods, the LIBS and NIRS characteristic variables of compound fertilizers are firstly screened by CARS algorithm, and then intermediate data fusion was carried out by using equal weight fusion or outer product fusion. Redundant variables in the original data can be well removed in the data fusion process to ensure the accuracy of the analysis. Therefore, the combined methods of LIBS-NIRS based on CARS-OPF and CARS-EWF could be well applied to the rapid and accurate detection of main elemental contents in compound fertilizers.