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1.
Methods Mol Biol ; 2196: 63-75, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32889713

RESUMO

Labeling a protein of interest is widely used to examine its quantity, modification, localization, and dynamics in the budding yeast Saccharomyces cerevisiae. Fluorescent proteins and epitope tags are often used as protein fusion tags to study target proteins. One prevailing technique is to fuse these tags to a target gene at the precise chromosomal location via homologous recombination. Here we describe a protein labeling strategy based on the URA3 pop-in/pop-out and counterselection system to fuse a fluorescent protein or epitope tag scarlessly to a target protein at its native locus in S. cerevisiae.

2.
Talanta ; 221: 121607, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33076137

RESUMO

Drug-induced liver injury (DILI) has been a hot issue of public health, owing to its unpredictability and serious harm to public health. Peroxynitrite (ONOO-) is an important biomarker for the assessment and diagnosis of DILI. In this article, based on a kind of rhodamine analogue with a near-infrared (NIR) emission (610 nm-800 nm) and a two-photon absorption cross section (54 GM), a two-photon excited NIR fluorescence probe (NIR-ONOO) for ONOO- was developed. With a high selectivity and a high sensitivity to ONOO-, NIR-ONOO has a linear range for detection of ONOO- from 5.0 × 10-8 to 1.0 × 10-5 M, a good detection limit (15 nM) and a large fluorescence enhancement (340-fold). In addition, NIR-ONOO has been used to monitor ONOO- in cells with satisfactory results. Because of its two-photon excied NIR emission, NIR-ONOO also showed excellent performances for imaging ONOO- including low autofluorescence, stable and persistent fluorescence, and a deep penetration (204 µm). Finally, NIR-ONOO was successfully employed to image ONOO- in inflammatory mouse, drug-induced hepatotoxicity in cells and its remediation. All the results indicated that NIR-ONOO is a powerful chemical tool to image ONOO- and assay drug-induced hepatotoxicity.

3.
J Cell Mol Med ; 2020 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-33043579

RESUMO

Endoplasmic reticulum (ER) stress and inflammatory responses play active roles in the transition of acute kidney injury (AKI) to chronic kidney disease (CKD). Inositol-requiring enzyme 1 (IRE1) activates c-Jun NH2 -terminal kinase (JNK) in ER stress. Tubular epithelial cells (TEC) are the main injury target and source of AKI inflammatory mediators. TEC injury may lead to glomerulosclerosis, however, the underlying mechanism remains unclear. Here, hypoxia/reoxygenation (H/R) HK-2 cells were used as an AKI model. To determine the partial effects of TEC injury on the glomerulus, HK-2 cells after H/R were co-cultured with human renal mesangial cells (HRMC). H/R up-regulated ER stress, IRE1/JNK pathway, IL-6 and MCP-1 in HK-2 cells. Stimulation of HRMC with IL-6 enhanced their proliferation and the expression of glomerulosclerosis-associated fibronectin and collagen IV via signal transducer and activator of transcription 3 (STAT3) activation. Similar responses were observed in HRMC co-cultured with HK-2 cells after H/R. IRE1/JNK inhibition reversed these injury responses in HRMC. IRE1/JNK stable knock-down in HK-2 cells and shRNA-mediated STAT3 depletion in HRMC confirmed their role in inflammation/glomerulosclerosis. These findings suggest that IRE1/JNK pathway mediates inflammation in TEC, affecting mesangial cells. The inhibition of this pathway could be a feasible approach to prevent AKI-CKD transition.

4.
Bioresour Technol ; 318: 124190, 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-33038621

RESUMO

Algal-bacterial aerobic granular sludge (AB-AGS) as a symbiosis system possesses high potential for being operated without external O2 supply. In this study, a novel lab-scale closed photo-sequencing batch reactor (PSBR) was developed for AB-AGS operation under successively open (Phase Ⅰ) and closed (Phase Ⅱ) conditions. Results show that AB-AGS maintained almost 100% of organics removal, exhibiting higher removals of phosphate (63 ± 20%), K+ (19 ± 12%) and Mg2+ (26 ± 12%), and higher chlorophylls content during Phase II. Meanwhile, only O2 besides N2 was detectable in the headspace of PSBR. The change of granular structure and faster algae growth during Phase Ⅱ may contribute to the increase of microbial activity and phosphorus bioavailability, in which lower extracellular polymeric substances content may account for low biomass retention. Results from this closed PSBR imply that AB-AGS has the potential to reduce some greenhouse gases like CO2 and CH4 emission.

5.
Zhongguo Yi Liao Qi Xie Za Zhi ; 44(5): 415-419, 2020 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-33047564

RESUMO

OBJECTIVE: To explore the integration method and technical realization of artificial intelligence bone age assessment system with the hospital RIS-PACS network and workflow. METHODS: Two sets of artificial intelligence based on bone age assessment systems (CHBoneAI 1.0/2.0) were developed. The intelligent system was further integrated with RIS-PACS based on the http protocol in Python flask web framework. RESULTS: The two sets of systems were successfully integrated into the local network and RIS-PACS in hospital. The deployment has been smoothly running for nearly 3 years. Within the current network setting, it takes less than 3 s to complete bone age assessment for a single patient. CONCLUSIONS: The artificial intelligence based bone age assessment system has been deployed in clinical RIS-PACS platform and the "running in parallel", which is marking a success of Stage-I and paving the way to Stage-II where the intelligent systems can evolve to become more powerful in particular of the system self-evolution and the "running alternatively".

6.
Zootaxa ; 4768(2): zootaxa.4768.2.9, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-33056529

RESUMO

Morphology and DNA barcodes confirm a new chironomid species within the Rheocricotopus (Psilocricotopus) orientalis group (Diptera: Chironomidae). Rheocricotopus (Psilocricotopus) kongi Lin et Wang sp. n. is described and illustrated based on adult male from Hainan, China. Key to adult males of the R. orientalis group is given.


Assuntos
Chironomidae , Animais , Código de Barras de DNA Taxonômico , Masculino
7.
Chemosphere ; 264(Pt 1): 128441, 2020 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-33032217

RESUMO

The adverse effects of antibiotics residues on aquaculture ecosystems and humans raised increasing concerns globally. To assess the occurrence, human health risks, sources, and bioaccumulation potential of antibiotics in cultured freshwater products in Eastern China, 12 and 13 aquaculture ponds were selected in 2018 and 2019, respectively, both covering 8 aquatic species. Concentrations of 12 commonly-used antibiotics were measured in muscle tissue of aquaculture products, water, sediment, and suspended particles. At least two antibiotics were found simultaneously in all muscle tissue samples. The concentrations of most antibiotics in freshwater cultured products were at a medium or lower level in comparison with other studies in China and worldwide, but slightly higher than the concentrations in cultured marine products. The potential risks from the intake of these aquatic products were also evaluated. The results showed limited adverse effects due to the consumption of these products with an exception of fluoroquinolone antibiotics. The bioaccumulation potential from water varied widely in different collection years, but the bioaccumulation factor (BAF) values for antibiotics were all <50 L/kg. BSAF values of antibiotics were all far below 1, except for one site in Zhejiang province in 2018, indicating that the bioavailability from surface sediments was low, in a particular pond environment. The low repeatability of BAF and BSAF calculated in two years indicated a relatively unsteady status in terms of bioaccumulation potential of cultured freshwater ponds yearly.

9.
Artigo em Inglês | MEDLINE | ID: mdl-33059922

RESUMO

Atherosclerosis is a chronic vascular inflammatory disease that initially starts from an arterial intima lesion and endothelial barrier dysfunction. The purpose of this study was to investigate the role of TM4SF19, a recently identified member of the transmembrane 4L six superfamily, in vascular endothelial cell adherens junctions. We found TM4SF19 expression was significantly increased in atherosclerotic plaques and sera of patients with coronary heart disease (CHD) compared with healthy people by immunohistochemistry and ELISA. In vitro, human umbilical vein endothelial cells (HUVECs) were stimulated by lipopolysaccharides (LPS). TM4SF19 and VE-cadherin expression as well as cell adherens junctions were assessed. Additionally, LPS could upregulate TM4SF19 expression and downregulate VE-cadherin expression in HUVECs in a concentration dependent manner. Overexpression of TM4SF19 substantially aggravated LPS-induced reduction of VE-cadherin expression and attenuation of vascular endothelial cell adherens junctions. However, both the decreased VE-cadherin expression and weakened cell adherens junctions induced by LPS could be dramatically reversed when the expression of TM4SF19 was depressed. This study is the first to reveal the effect of TM4SF19 on endothelial cell adherens junctions. Meanwhile, our results also provide novel therapeutic strategies for atherosclerotic diseases.

10.
Clin Rheumatol ; 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33067770

RESUMO

OBJECTIVE: This study aimed to identify the clinical characteristics of systemic lupus erythematosus-primary biliary cholangitis (SLE-PBC) patients and to compare the manifestations and prognosis with systemic lupus erythematosus (SLE) patients. METHODS: Twenty-one inpatients with concomitant SLE and primary biliary cholangitis (PBC) were identified in our hospital. Baseline clinical manifestations, laboratory results, disease activity, and organ damage, as well as changes in disease manifestations and therapies, were retrospectively analyzed. Baseline clinical characteristics, survival rate, and flare-ups were compared with 254 SLE patients also from our hospital. RESULTS: The prevalence of concomitant PBC in SLE inpatients was 0.27%. Over half of the patients were diagnosed with SLE and PBC simultaneously. Compared with SLE patients, SLE-PBC patients started the symptom of SLE at an older age, with a longer delay before the diagnosis of SLE (P < 0.05). Hematological and muscular involvement, pulmonary arterial hypertension, and interstitial lung disease were more common in SLE-PBC patients (P < 0.05). Kaplan-Meier estimate showed a significantly lower survival rate in SLE-PBC group, with 3-year survival rate at 88.4%. CONCLUSION: Concomitant PBC might have a negative impact on the survival of SLE, with older age at SLE onset, longer delay before SLE diagnosis, and higher baseline damage. More intensive therapy and prevention of hepatic toxicity need to be considered. Key Points • Hematological and muscular involvement, PAH, and ILD were more common in SLE PBC than in SLE. • The study firstly reported the survival rate of SLE PBC patients. • More intensive therapy and prevention of hepatic toxicity are needed for SLE-PBC.

11.
Epidemiol Infect ; 148: e241, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32998791

RESUMO

A recently developed pneumonia caused by SARS-CoV-2 has quickly spread across the world. Unfortunately, a simplified risk score that could easily be used in primary care or general practice settings has not been developed. The objective of this study is to identify a simplified risk score that could easily be used to quickly triage severe COVID-19 patients. All severe and critical adult patients with laboratory-confirmed COVID-19 on the West campus of Union Hospital, Wuhan, China, from 28 January 2020 to 29 February 2020 were included in this study. Clinical data and laboratory results were obtained. CURB-65 pneumonia score was calculated. Univariate logistic regressions were applied to explore risk factors associated with in-hospital death. We used the receiver operating characteristic curve and multivariate COX-PH model to analyse risk factors for in-hospital death. A total of 74 patients (31 died, 43 survived) were finally included in the study. We observed that compared with survivors, non-survivors were older and illustrated higher respiratory rate, neutrophil-to-lymphocyte ratio, D-dimer and lactate dehydrogenase (LDH), but lower SpO2 as well as impaired liver function, especially synthesis function. CURB-65 showed good performance for predicting in-hospital death (area under curve 0.81, 95% confidence interval (CI) 0.71-0.91). CURB-65 ⩾ 2 may serve as a cut-off value for prediction of in-hospital death in severe patients with COVID-19 (sensitivity 68%, specificity 81%, F1 score 0.7). CURB-65 (hazard ratio (HR) 1.61; 95% CI 1.05-2.46), LDH (HR 1.003; 95% CI 1.001-1.004) and albumin (HR 0.9; 95% CI 0.81-1) were risk factors for in-hospital death in severe patients with COVID-19. Our study indicates CURB-65 may serve as a useful prognostic marker in COVID-19 patients, which could be used to quickly triage severe patients in primary care or general practice settings.

12.
Int Heart J ; 61(5): 1005-1013, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32999188

RESUMO

This study sought to evaluate clinical features, treatment patterns, and outcomes of patients with idiopathic inflammatory myopathy (IIM) complicated by heart failure (HF). Thirty-two patients with IIM-HF admitted to the Peking Union Medical College Hospital between January 1999 and January 2018 were retrospectively reviewed, including 14 patients with polymyositis, 11 with dermatomyositis, and 7 with overlap syndrome. Survivors and no-survivors were compared on clinical characteristics and treatment. Although systemic symptoms were variable, all patients presented with elevated troponin I. Rapid atrial arrhythmia was the most frequent arrhythmia. Systolic dysfunction and restrictive diastolic dysfunction were typical presentations in echocardiography. Twenty-nine patients were followed up for a median of 2.8 years (0.1 month to 11 years). We recorded 13 deaths of cardiogenic cause, 1 of serious IIM, and 3 of infective complications. The median survival time from diagnosis of IIM-HF to all-cause mortality was 8.4 months (range from 1 month to 5 years). Both all-cause deaths and cardiogenic deaths were more reported in the methotrexate-alone group than in the combination therapy group (6/7 versus 3/10, P = 0.050; 5/6 versus 2/9, P = 0.041). Combination therapy including methotrexate (HR = 0.188, 95%CI 0.040-0.871, P = 0.033) and taking ß-receptor blockers (HR = 0.249, 95%CI 0.086-0.719, P = 0.010) was associated with reduced risk of all-cause deaths. In conclusion, elevated troponin I, atrial arrhythmia, and systolic and restrictive diastolic dysfunction are typical characteristics of IIM-HF. Combined immunosuppression that includes methotrexate and ß-receptor blockers seems to be important to improve survival.


Assuntos
Imunossupressores/uso terapêutico , Metotrexato/uso terapêutico , Miosite/tratamento farmacológico , Miosite/mortalidade , Adolescente , Antagonistas Adrenérgicos beta/uso terapêutico , Adulto , Idoso , China/epidemiologia , Quimioterapia Combinada , Feminino , Glucocorticoides/uso terapêutico , Insuficiência Cardíaca/etiologia , Humanos , Imunoglobulinas Intravenosas/uso terapêutico , Masculino , Metilprednisolona/uso terapêutico , Pessoa de Meia-Idade , Miosite/complicações , Estudos Retrospectivos , Adulto Jovem
13.
Chemosphere ; 264(Pt 2): 128534, 2020 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-33045508

RESUMO

Biological phosphorus removal (BPR) from wastewater can be generally realized through alternative non-aeration and aeration operation to create anaerobic and aerobic conditions respectively for P release and uptake/accumulation by polyphosphate accumulating organisms (PAOs), with P removal finally achieved by controlled discharge of P-rich sludge. In this study, the response of algal-bacterial aerobic granular sludge (AB-AGS) during BPR to main ions including Ac- (acetate), Cl-, SO42-, NH4+, K+, Mg2+, Ca2+ and Na+ in wastewater was investigated with conventional bacterial AGS (B-AGS) as control and acetate as the sole carbon source. Results show that BPR process mainly involved the changes of Ac-, K+, Mg2+, and Ca2+ rather than Cl-, SO42-, NH4+ and Na+. The mole ratio of ΔP/ΔAc kept almost unchanged during the non-aeration (P release) phase in both B-AGS and AB-AGS systems (ΔPB-AGS/ΔAcB-AGS > ΔPAB-AGS/ΔAcAB-AGS), and it was negatively influenced by the light in AB-AGS systems, in which 62% of acetate was not utilized for P release at the high illuminance of 81 k lux. During the entire non-aeration/aeration period, both ΔK/ΔP and ΔMg/ΔP remained constant, while ΔKAB-AGS/ΔPAB-AGS > ΔKB-AGS/ΔPB-AGS and ΔMgAB-AGS/ΔPAB-AGS ≈ ΔMgB-AGS/ΔPB-AGS. The presence of algae seemed not beneficial for PAOs to remove P, while more K+ and P uptake by algae in AB-AGS suggest its great potential for manufacturing biofertilizer.

14.
BMC Psychiatry ; 20(1): 484, 2020 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-33008339

RESUMO

BACKGROUND: The 7-item Gaming Addiction Scale (GAS) has been used as a screening tool for addictive game use worldwide, and this study aimed to examine its psychometric properties and measurement invariance among college students in China. METHODS: Full-time students from multiple colleges in China were recruited. A total of 1040 completed questionnaires were used in the final analysis. Reliability of the GAS was assessed by internal consistency and split-half reliability. Validity of the GAS was assessed by structural validity, convergent validity, discriminant validity, and concurrent validity. A series of Multigroup Confirmatory Factor Analysis (MG-CFA) were conducted to test and establish measurement invariance across gender, class standing, family income and parental educational level. RESULTS: Exploratory factor analysis revealed a unidimensional structure of the GAS. The GAS exhibited excellent internal consistency (Cronbach's α = 0.951, theta coefficient = 0.953, omega coefficient = 0.959) and structural validity (χ2 /df = 0.877 (p < 0.05), CFI = 0.999, TIL = 0.996, RMSEA =0.000). Concurrent validity of the GAS was confirmed by its correlation with problematic internet use, sleep quality, nine dimensions of psychiatric symptoms, and substance use. The GAS also demonstrated measurement invariance across father's educational level (Δχ2 (df) = 19.128 (12), ΔCFI = - 0.009, ΔRMSEA = 0.010 for weak factorial model; Δχ2 (df) = 50.109 (42), ΔCFI = - 0.010, ΔRMSEA = 0.007 for strict factorial model.) and mother's educational level (Δχ2 (df) = 6.679 (12), ΔCFI = 0.007, ΔRMSEA = - 0.010 for weak factorial model; Δχ2 (df) =49.131 (42), ΔCFI = - 0.009, ΔRMSEA = - 0.004 for strict factorial model), as well as partial measurement invariance across gender (except for item 2), class standing (except for item 7) and family income (except for item 5). CONCLUSIONS: The Chinese version of the 7-item GAS can be an adequate assessment tool to assess internet gaming disorder among the college student population in China.

15.
J Diabetes Res ; 2020: 3634974, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33015191

RESUMO

Tangshen Formula (TSF) is a Chinese Medicine formula that has been reported to alleviate proteinuria and protect renal function in humans and animals with diabetic kidney disease (DKD). However, little is known about its mechanism in improving proteinuria. The dysregulation of podocyte cell-matrix adhesion has been demonstrated to play an important role in the pathogenesis and progression of proteinuric kidney diseases including DKD. In the present study, the underlying protective mechanism of TSF on podocytes was investigated using the murine model of type 2 DKD db/db mice in vivo and advanced glycation end products (AGEs)-stimulated primary mice podocytes in vitro. Results revealed that TSF treatment could significantly mitigate reduction of podocyte numbers and foot process effacement, reduce proteinuria, and protect renal function in db/db mice. There was a significant increase in expression of transient receptor potential canonical channel 6 (TRPC6) and a decrease in expression of talin1 in podocytes of db/db mice. The results of AGEs-stimulated primary mice podocytes showed increased cell migration and actin-cytoskeleton rearrangement. Moreover, primary mice podocytes stimulated by AGEs displayed an increase in TRPC6-dependent Ca2+ influx, a loss of talin1, and translocation of nuclear factor of activated T cell (NFATC) 2. These dysregulations in mice primary podocytes stimulated by AGEs could be significantly attenuated after TSF treatment. 1-Oleoyl-2-acetyl-sn-glycerol (OAG), a TRPC6 agonist, blocked the protective role of TSF on podocyte cell-matrix adherence. In conclusion, TSF could protect podocytes from injury and reduce proteinuria in DKD, which may be mediated by the regulation of the TRPC6/Talin1 pathway in podocytes.

16.
J Clin Pathol ; 2020 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-33020176

RESUMO

AIMS: A growing research demonstrated that YAP1 played important roles in gliomagenesis. We explored the expression of YAP1 and STAT3, the relationship between them and the effect of YAP1, STAT3 on prognosis in glioma. METHODS: Expression of YAP1, p-YAP1, STAT3, pSTAT3-S727 and pSTAT3-Y705 in 141 cases of low-grade gliomas (LGG) and 74 cases of high-grade gliomas (HGG) of surgical specimens were measured by immunohistochemistry. Pearson's X2 test was used to determine the correlation between immunohistochemical expressions and clinicopathological parameters. Pearson's or Spearman correlation test was used to determine the association between these proteins expression. Survival analysis was used to investigate the effect of these proteins on prognosis. RESULTS: High expressions of YAP1, STAT3, pSTAT3-S727 and pSTAT3-Y705 were found in HGG compared with LGG (p=0.000). High expressions of YAP1, STAT3, pSTAT3-S727 and pSTAT3-Y705 were found in 63.5%, 59.5%, 66.2% and 31.1% cases of HGG, respectively. YAP1 expression was associated to tumour location, Ki-67 and P53, STAT3 expression was related with Ki-67 and P53, and the expression of pSTAT3-S727 was associated with Ki-67. There was a significantly positive correlation between YAP1 and pSTAT3-S727 (p<0.0001; r=0.5663). Survival analysis revealed that patients with YAP1 and pSTAT3-S727 coexpression had worse overall survival (OS) and progression-free survival (PFS) (p<0.0001). Tumour grade, age, Ki-67 and YAP1 expression were independent prognostic factors for OS. In LGG group, both YAP1 and pSTAT3-S727 expressions were negative correlation with IDH1 mutation, YAP1 and pSTAT3-S727 coexpression showed worse OS and PFS of glioma patients. CONCLUSION: Our research showed that YAP1 and STAT3 were significantly activated in HGG compared with LGG. YAP1 significantly correlated with pSTAT3-S727 in glioma, YAP1 and pSTAT3-S727 coexpression may serve as a reliable prognostic biomarker and therapeutic target for glioma.

17.
BJOG ; 2020 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-33021043

RESUMO

OBJECTIVE: This study aimed to investigate the incidence and associated predictors of adverse pregnancy outcomes (APOs) among pregnant women infected with syphilis. DESIGN: Cox regression analysis. SETTING: China. POPULATION OR SAMPLE: Pregnant women who were tested and diagnosed with syphilis during the index pregnancy and delivered at a gestational age ≥28 weeks between 2016 and 2019. METHODS: Data were extracted from China's Information System of Prevention of Mother-to-Child Transmission (PMTCT) of Syphilis Management. Descriptive analysis provided profiles and pregnant outcomes of maternal syphilis, as well as the incidence of APOs. Log-rank tests and cox proportional hazard models were used to investigate factors influencing APOs in infected mothers with singleton births. MAIN OUTCOME MEASURES: The incidence of APOs; the Hazard Ratios (HRs) of associated predictors using cox proportional hazard model. RESULTS: Syphilis treatment data was available from 83.86% of diagnosed women. Including deliveries from the total study population, 13.33% experienced APOs. Cox regression indicated that APOs were more likely in women tested and diagnosed in the late trimester, delivery or postpartum. Women who accepted non-standardized treatment and who received standardized treatment had less risk of APOs . CONCLUSIONS: China has made huge progress over the last decades in PMTCT of syphilis, but the incidence of APOs among pregnant women infected with syphilis remains high. It is extremely essential to further strengthen access to early detection and standardized treatment of infected women to reduce the risk of APOs.

18.
Chem Commun (Camb) ; 56(79): 11827-11830, 2020 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-33021254

RESUMO

Herein, novel coin tree-like TiO2 moieties with lots of anatase-rutile phase junctions were constructed by a general, simple, and environmentally friendly strategy. The anatase/rutile ratios can be easily tuned by changing the ratios of the two H-bond donors. Owing to this featured shape, the TiO2 sample displays robust photocatalytic activity and better stability.

19.
J Mater Chem B ; 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33021617

RESUMO

Correction for 'Enabling nanopore technology for sensing individual amino acids by a derivatization strategy' by Xiaojun Wei et al., J. Mater. Chem. B, 2020, 8, 6792-6797, DOI: 10.1039/D0TB00895H.

20.
Oncol Rep ; 44(5): 1997-2008, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33000266

RESUMO

Curcumin is the main component of the Chinese herbal plant turmeric, which has been demonstrated to possess antitumor and other pharmacological properties. The aim of the present study was to investigate the effects of curcumin on the viability, migration and apoptosis of human colorectal carcinoma HCT­116 cells, and to explore the underlying molecular mechanisms. In addition, it was investigated whether the antitumor effect of curcumin on HCT­116 cells could match that of the chemotherapeutic drug 5­fluorouracil (5­FU). HCT­116 cells were treated with curcumin (10, 20 and 30 µM) and 5­FU (500 µM), and cell viability and proliferation were detected by Cell Counting Kit­8 and colony formation assays, respectively. The migration and invasion of treated cells were determined using Transwell and carboxyfluorescein succinimidyl amino ester fluorescent labeling assays. Cell cycle distribution and apoptosis rates were detected by flow cytometry. Furthermore, cell morphology changes associated with apoptosis were observed by fluorescence microscopy with acridine orange/ethidium bromide dual staining. To investigate the possible underlying molecular mechanisms, the gene and protein levels of Fas, Fas­associated via death domain (FADD), caspase­8, caspase­3, matrix metalloproteinase (MMP)­9, nuclear factor (NF)­κB, E­cadherin and claudin­3 were detected using quantitative PCR analysis, zymography and western blotting. The results revealed that curcumin markedly inhibited the viability and proliferation of HCT­116 cells in a dose­ and time­dependent manner. The migration, aggregation and invasion of HCT­116 cells into the lungs of mice were decreased by curcumin treatment in a dose­dependent manner. S­phase arrest and gradually increased apoptotic rates of HCT­116 cells were observed with increasing curcumin concentrations. Additionally, the mRNA and protein levels of apoptosis­associated proteins (Fas, FADD, caspase­8 and caspase­3) and E­cadherin in HCT­116 cells were upregulated following treatment with curcumin in a dose­dependent manner. By contrast, the expression of migration­associated proteins, including MMP­9, NF­κB and claudin­3, was downregulated with increasing curcumin concentrations. These data suggested that the inhibitory effect of curcumin on HCT­116 cells may match that of 5­FU. Therefore, curcumin induced cell apoptosis and inhibited tumor cell metastasis by regulating the NF­κB signaling pathway, and its therapeutic effect may be comparable to that of 5­FU.

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