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1.
Medicine (Baltimore) ; 100(18): e25829, 2021 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-33950992

RESUMO

BACKGROUND: Dental caries is a common disease under the action of many factors. Dental caries may occur in all age groups, among which children and adolescents are at high risk of dental caries. Early identification of the risk factors of dental caries is very important for clinical staff to prevent and intervene as soon as possible and reduce the incidence of dental caries. Although Chinese scholars have studied and summarized the risk factors of dental caries in children and adolescents, the conclusions are different. Therefore, in this study, meta-analysis was used to summarize the risk factors of dental caries in Chinese children and adolescents, and to explore the characteristics of high-risk groups of dental caries, so as to provide reference for early detection and prevention of dental caries. METHODS: Medical specialty databases like PubMed, Embase, Cochrane Library, Web of Science, China Biology Medicine Database, China National Knowledge Infrastructure, China Science and Technology Journal Database, and Wanfang Database were consistently and exhaustively searched. According to the method of evidence-based medicine, the inclusion and exclusion criteria were established, and the meta-analysis of all eligible research results was carried out by using Review Manager 5.3 software. RESULTS: We will disseminate the findings of this systematic review and meta-analysis via publications in peer-reviewed journals. CONCLUSIONS: Our study was carried out to estimate the pooled prevalence of dental caries and its associated factors among Chinese children and adolescents. OSF REGISTRATION NUMBER: DOI 10.17605/OSF.IO/RA9D6.


Assuntos
Cárie Dentária/epidemiologia , Adolescente , Criança , China/epidemiologia , Humanos , Metanálise como Assunto , Prevalência , Fatores de Risco , Revisões Sistemáticas como Assunto
2.
Dev Biol ; 402(1): 72-80, 2015 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-25794679

RESUMO

Each skeletal muscle contains a fixed ratio of fast and slow myofibers that are distributed in a stereotyped pattern to achieve a specific motor function. How myofibers are specified during development and regeneration is poorly understood. Here we address this question using transgenic reporter mice that indelibly mark the myofiber lineages based on activation of fast or slow myosin. Lineage tracing indicates that during development all muscles have activated the fast myosin gene Myl1, but not the slow myosin gene Myh7, which is activated in all slow but a subset of fast myofibers. Similarly, most nascent myofibers do not activate Myh7 during fast muscle regeneration, but the ratio and pattern of fast and slow myofibers are restored at the completion of regeneration. At the single myofiber level, most mature fast myofibers are heterogeneous in nuclear composition, manifested by mosaic activation of Myh7. Strikingly, Myh7 is activated in a subpopulation of proliferating myoblasts that co-express the myogenic progenitor marker Pax7. When induced to differentiate, the Myh7-activated myoblasts differentiate more readily than the non-activated myoblasts, and have a higher tendency, but not restricted, to become slow myotubes. Together, our data reveal significant nuclear heterogeneity within a single myofiber, and challenge the conventional view that myosin genes are only expressed after myogenic differentiation. These results provide novel insights into the regulation of muscle fiber type specification.


Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Fibras Musculares de Contração Lenta/metabolismo , Músculos/citologia , Músculos/metabolismo , Mioblastos/citologia , Cadeias Pesadas de Miosina/metabolismo , Animais , Cardiotoxinas/química , Diferenciação Celular , Núcleo Celular/metabolismo , Separação Celular , Células Cultivadas , Citometria de Fluxo , Genes Reporter , Proteínas de Fluorescência Verde/metabolismo , Camundongos , Camundongos Transgênicos , Músculo Esquelético/metabolismo , Músculos/patologia , Miosinas/química , Fator de Transcrição PAX7/metabolismo , Regeneração , Células Satélites de Músculo Esquelético/citologia
3.
Int J Clin Exp Pathol ; 7(11): 7451-9, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25550780

RESUMO

OBJECTIVE: This study aims to explore the function of Integrin-ß/FAK in the mechanical signal transduction and the connection with downstream ERK signal pathways. METHODS: Human osteosarcoma MG63 cell lines were used in this study. The effects of mechanical strain on the Integrin-ß1 expression, FAK and ERK signal pathway in Human osteosarcoma MG63 cells were detected using RT-PCR and Western-blotting methods. The localization of FAK in Human osteosarcoma MG63 cells were determined using immunofluorescent method. The interaction between Integrin-ß1 and FAK were detected by using co-immunoprecipitation method. RESULTS: The expression of Integrin-ß1 shows a notable bimodel distribution, mechanical strain stimulation can promote Integrin-ß1 expression and the phosphorylation of FAK and ERK, mechanical strain activated FAK and ERK mediated by Integrin-ß1. CONCLUSION: Integrin-ß1 may play an important role in osteoblast proliferation differentiation process, it might feel external strain stimulation through ECM composition and makes FAK phosphated through the interaction with FAK, thus causing a series of activation of signal molecules. Finally it reduces MAPK (ERK) activation and cellular responses to finish mechanical signal transduction.


Assuntos
Quinase 1 de Adesão Focal/metabolismo , Integrina beta1/metabolismo , Sistema de Sinalização das MAP Quinases , Diferenciação Celular , Linhagem Celular Tumoral , Proliferação de Células , Quinase 1 de Adesão Focal/genética , Humanos , Integrina beta1/genética , Fosforilação
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