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1.
Chem Commun (Camb) ; 2022 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-35920312

RESUMO

Fe, Mo-doped Ni3S2 nanosheets that are derived from NiFe-LDH by structural transformation have been successfully developed. The obtained Fe, Mo-Ni3S2 exhibits a low overpotential of 67 mV to enable a current density of 10 mA cm-2 for the HER and the overpotential for the OER is only 240 mV. Besides, the current density of 10 mA cm-2 can be achieved with a voltage of 1.53 V in a two-electrode hydrolysis device.

2.
J Environ Sci (China) ; 119: 106-118, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35934455

RESUMO

The gaseous or particulate forms of divalent mercury (HgII) significantly impact the spatial distribution of atmospheric mercury concentration and deposition flux (FLX). In the new nested-grid GEOS-Chem model, we try to modify the HgII gas-particle partitioning relationship with synchronous and hourly observations at four sites in China. Observations of gaseous oxidized Hg (GOM), particulate-bound Hg (PBM), and PM2.5 were used to derive an empirical gas-particle partitioning coefficient as a function of temperature (T) and organic aerosol (OA) concentrations under different relative humidity (RH). Results showed that with increasing RH, the dominant process of HgII gas-particle partitioning changed from physical adsorption to chemical desorption. And the dominant factor of HgII gas-particle partitioning changed from T to OA concentrations. We thus improved the simulated OA concentration field by introducing intermediate-volatility and semi-volatile organic compounds (I/SVOCs) emission inventory into the model framework and refining the volatile distributions of I/SVOCs according to new filed tests in the recent literatures. Finally, normalized mean biases (NMBs) of monthly gaseous element mercury (GEM), GOM, PBM, WFLX were reduced from -33%-29%, 95%-300%, 64%-261%, 117%-122% to -13%-0%, -20%-80%, -31%-50%, -17%-23%. The improved model explains 69%-98% of the observed atmospheric Hg decrease during 2013-2020 and can serve as a useful tool to evaluate the effectiveness of the Minamata Convention on Mercury.


Assuntos
Poluentes Atmosféricos , Mercúrio , Aerossóis , Poluentes Atmosféricos/análise , Poeira , Monitoramento Ambiental/métodos , Gases , Mercúrio/análise
4.
Artigo em Inglês | MEDLINE | ID: mdl-35931383

RESUMO

BACKGROUND & AIMS: The prevalence of nonalcoholic fatty liver disease (NAFLD) has reached epidemic proportions globally as a result of the rapid increase in obesity. However, there is no Food and Drug Administration-approved pharmacotherapy available for NAFLD. This study investigated the role of autotaxin, a secreted enzyme that hydrolyzes lysophosphatidylcholine to produce lysophosphatidic acid (LPA), in the pathogenesis of NAFLD and to explore whether genetic or pharmacologic interventions targeting autotaxin ameliorate NAFLD. METHODS: The clinical association of autotaxin with the severity of NAFLD was analyzed in 125 liver biopsy-proven NAFLD patients. C57BL/6N mice or fibroblast growth factor 21 (FGF21)-null mice were fed a high-fat diet or a choline-deficient diet to investigate the role of the autotaxin-FGF21 axis in NAFLD development by hepatic knockdown and antibody neutralization. Huh7 cells were used to investigate the autocrine effects of autotaxin. RESULTS: Serum autotaxin levels were associated positively with histologic scores and NAFLD severity. Hepatocytes, but not adipocytes, were the major contributor to increased circulating autotaxin in both patients and mouse models with NAFLD. In mice, knocking-down hepatic autotaxin or treatment with a neutralizing antibody against autotaxin significantly reduced high-fat diet-induced NAFLD and high fat- and choline-deficient diet-induced nonalcoholic steatohepatitis and fibrosis, accompanied by a marked increase of serum FGF21. Mechanistically, autotaxin inhibited the transcriptional activity of peroxisome proliferator-activated receptor α through LPA-induced activation of extracellular signal-regulated kinas, thereby leading to suppression of hepatic FGF21 production. The therapeutic benefit of anti-autotaxin neutralizing antibody against NAFLD was abrogated in FGF21-null mice. CONCLUSIONS: Liver-secreted autotaxin acts in an autocrine manner to exacerbate NAFLD through LPA-induced suppression of the peroxisome proliferator-activated receptor α-FGF21 axis and is a promising therapeutic target for NAFLD.

5.
Int J Biol Macromol ; 2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-35963353

RESUMO

Hydrogels are attractive soilless media for plant cultivation with strong water and nutrient retention. However, pristine hydrogels contain mostly ultra-micro pores and lack air-filled porosity for root zone aeration. Herein we report a porous hydrogel composite comprising an agarose network and porous growing mix particle (GMP) fillers. The agarose backbone allowed the composite to sustain a 12-d growth cycle for red cabbage microgreens without the need for watering or crew interaction. Moreover, the GMP induced greater total pore volume and increased the prevalence of pores >30 µm by 8-fold. Further investigation suggested that the nutrients from GMP accounted for a 54 % increase in microgreen yield over pristine hydrogel, while the porous structure introduced by GMP improved the yield by another 44 %. Increased air-filled porosity accelerated the water transport and loss of hydrogel but maintained favorable water potential levels for plant extraction. Finally, the hydrogel composite supported microgreen growth satisfyingly under simulated microgravity despite some morphological changes. Results of this study reveal a novel growth substrate that is lightweight, convenient, and water-efficient, while effectively sustaining plant growth for multiple applications including indoor farming and space farming.

6.
J Cell Sci ; 2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-35946425

RESUMO

Mitral and tricuspid valves are essential for unidirectional blood flow in the heart. They are derived from similar cell sources, and yet congenital dysplasia affecting both valves is clinically rare, suggesting the presence of differential regulatory mechanisms underlying their development. We specifically inactivated Dicer1 in the endocardium during cardiogenesis and found that Dicer1-deletion caused congenital mitral valve stenosis and regurgitation, while it had no impact on other valves. We showed that hyperplastic mitral valves were caused by abnormal condensation and extracellular matrix (ECM) remodeling. Our single-cell RNA Sequencing analysis revealed impaired maturation of mesenchymal cells and abnormal expression of ECM genes in mutant mitral valves. Furthermore, expression of a set of miRNAs that target ECM genes was significantly lower in tricuspid valves compared to mitral valves, consistent with the idea that the miRNAs are differentially required for mitral and tricuspid valve development. We thus reveal miRNA-mediated gene regulation as a novel molecular mechanism that differentially regulates mitral and tricuspid valve development, thereby enhancing our understanding of the non-association of inborn mitral and tricuspid dysplasia observed clinically.

7.
Front Immunol ; 13: 830606, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35935985

RESUMO

Accumulating evidence suggests that regulatory B cells (Bregs) play important roles in inhibiting the immune response in tumors. Programmed death 1 (PD-1) and programmed death ligand 1 (PD-L1) are important molecules that maintain the balance of the immune response and immune tolerance. This study aims to evaluate the soluble form of PD-L1 and its function in inducing the differentiation of B lymphocytes, investigate the relationship between soluble PD-L1 (sPD-L1) and B-cell subsets, and explore the antitumor activity of T lymphocytes after PD-L1 blockade in coculture systems. In an effort to explore the role of sPD-L1 in human breast cancer etiology, we examined the levels of sPD-L1 and interleukin-10 (IL-10) in the serum of breast tumor patients and the proportions of B cells, PD-1+ B cells, Bregs, and PD-1+ Bregs in the peripheral blood of patients with breast tumors and assessed their relationship among sPD-L1, IL-10, and B-cell subsets. The levels of sPD-L1 and IL-10 in serum were found to be significantly higher in invasive breast cancer (IBCa) patients than in breast fibroadenoma (FIBma) patients. Meanwhile, the proportions and absolute numbers of Bregs and PD-1+ Bregs in the peripheral blood of IBCa patients were significantly higher than those of FIBma patients. Notably, they were the highest in triple-negative breast cancer (TNBC) among other subtypes of IBCa. Positive correlations of sPD-L1 and IL-10, IL-10 and PD-1+ Bregs, and also sPD-L1 and PD-1+ Bregs were observed in IBCa. We further demonstrated that sPD-L1 could induce Breg differentiation, IL-10 secretion, and IL-10 mRNA expression in a dose-dependent manner in vitro. Finally, the induction of regulatory T cells (Tregs) by Bregs was further shown to suppress the antitumor response and that PD-L1 blockade therapies could promote the apoptosis of tumor cells. Together, these results indicated that sPD-L1 could mediate the differentiation of Bregs, expand CD4+ Tregs and weaken the antitumor activity of CD4+ T cells. PD-L1/PD-1 blockade therapies might be a powerful therapeutic strategy for IBCa patients, particularly for TNBC patients with high level of PD-1+ Bregs.


Assuntos
Linfócitos B Reguladores , Síndromes de Imunodeficiência , Neoplasias de Mama Triplo Negativas , Antígeno B7-H1/metabolismo , Humanos , Tolerância Imunológica , Terapia de Imunossupressão , Interleucina-10 , Receptor de Morte Celular Programada 1/metabolismo
8.
China CDC Wkly ; 4(26): 577-581, 2022 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-35919456

RESUMO

Air pollution is a significant risk factor contributing to the burden of disease in China. Health risk assessment and management are important to reduce the impact of air pollution on public health. To help formulate standardized health risk assessment techniques, a series of studies were conducted from 2006 to 2019. Through systematic review, study of molecular mechanisms, epidemiological investigation, and health effect monitoring, the overall project established a monitoring and evaluation indicator system, a comprehensive information platform, software for automatic data cleaning, and standardized health risk assessment techniques. Technical specifications have been issued by the National Health Commission for promoting health risk assessments across China. This paper introduces the project, the research approach, its main research accomplishments, innovations, and public health significance, and describes directions for further research.

9.
Artigo em Inglês | MEDLINE | ID: mdl-35911140

RESUMO

Type 2 diabetes mellitus (T2DM), a common disease with a complex etiology in the world, is an important risk factor for severe cardiovascular and cerebrovascular diseases. However, treatments of T2DM are mainly based on Western medicine, whose severe side effects make traditional Chinese medicine (TCM) therapy more appealing to patients and clinicians. The overall clinical evidence for different TCM therapies in the treatment of T2DM is still unclear. This study aimed to adopt the evidence-mapping method and integrate the evidence from various researches on this topic, to depict the whole picture of TCM therapies for T2DM. This review included searches of PubMed, Embase, Web of Science, and three major Chinese literature databases (CNKI, VIP, and Wanfang) from inception to November 18, 2021. Two independent reviewers screened the literature, extracted information, and evaluated the quality of all included studies. A systematic review was subsequently performed. In total, 47 studies were reviewed, of which 46 studies (97.9%) were from China and 1 (2.1%) was from Canada. The evidence map was conducted according to different TCM therapies, including herbs or herbal extracts, compounds, powders, decoctions, pills, external treatment, basic theories and treatment principles of TCM, proprietary Chinese medicines, and unspecified TCM integrated therapies. According to the AMSTAR-2 scoring results, 4 papers were rated as high quality, 11 were low quality, and 32 were very low quality. Outcome indicators mainly focused on FBG, HbA1c, 2-h PBG, TC, TG, LDL-C, etc. The results showed that different types of TCM treatment had different improvement effects on the outcome indicators of T2DM. More consistent benefits were observed in the improvement of FBG, HbA1c, and 2-h PBG with treatment regimens based on basic theories and treatment principles of TCM, decoctions and pills, and unspecified TCM integrated therapies. Among herbs, ginger and Coptis root showed more improvement in all outcomes. Compounds, powders, and external treatment showed relatively consistent beneficial effects on the improvement of FBG. No serious adverse events were reported. Overall, the current evidence map provided an intuitive overview of the beneficial effects of TCM therapies in the treatment of type 2 diabetes. This study can be used as a reference for the clinical application of traditional Chinese medicine in T2DM, but due to the low-quality level of the included studies, it should be treated with caution in clinical practices.

10.
Food Funct ; 2022 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-35917196

RESUMO

Pixian broad-bean paste (PBBP) is a famous fermented condiment in China, which may produce abundant flavor peptides during the fermentation process. Herein, the flavor peptides from fermented broad-bean (FB) were separated and identified via ethanol precipitation, macroporous resin, and ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) methods. The results showed that there were fifteen novel flavor peptides in FB. Among them, six peptides (i.e. ALDELGT, AELTPEP, SAALQAG, SFEAVEAAPT, EQDNDGNNIFSGFKR, QTFNTEEDTAK, and DAPASGN) were perceived umami with threshold values ranging from 0.76 to 1.84 mmol L-1. And they had both salty-enhancing and umami-enhancing abilities. Meanwhile, novel peptides (i.e. GGLRIINPEGQQ, SIITPPERQ, GGLSIITPPERQA, GGLRIINPEG, SIITPPER, RIINPEGQQ, DALNVNRHIV, and LPKILLLQLV) were perceived bitter with threshold values ranging from 0.56 to 2.89 mmol L-1. And the monosodium glutamate (MSG) solution had a certain masking effect on bitter peptides. GFSSEFLA showed a strong sweet taste. From peptide sequence analysis, it was found that Aspergillus oryzae and broad-bean were important taste-active sources of the flavor peptides in FB. Besides, the synthetic peptides showed a much better sensory taste than the mixtures of the corresponding amino acids. It indicated that peptides might play an important role in deciding the taste of FB. Significantly, it is the first time that the isolated flavor peptides were identified from FB.

11.
Int J Soc Psychiatry ; : 207640221109168, 2022 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-35943191

RESUMO

BACKGROUND: Schizophrenia is a chronic and severe mental disorder. People with schizophrenia have transferred from hospital-based care to community-based care with the support of mental health legal policies. Challenges faced in the community should be emphasized. Limited qualitative studies have explored the challenges of living with schizophrenia. AIMS: To explore the challenges of people living with schizophrenia in the community. METHODS: A narrative method was used, including semi-structured, face-to-face interviews. Thematic analysis approach was used to analyze data. RESULTS: Ten clients and their family members participated in the study. Analysis revealed three main themes related to their challenges in the community: deficits in self-management of illness; insufficient community mental health care; and the influence of policy. These challenges prevent those with schizophrenia from effectively managing their illness, returning to a productive role in society, and improving their quality of life. CONCLUSIONS: There are challenges in mental health rehabilitation and social reintegration of individuals with schizophrenia. There is a need for continuous community mental rehabilitation services, appropriate policy support, and the need to educate the public to reduce social bias and discrimination which allows individuals with schizophrenia to assume a productive role in the community.

12.
Sci Rep ; 12(1): 13613, 2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-35948587

RESUMO

In this paper, three monitoring sections were set up in Lugu Lake, and water samples were collected in 2019, 2020, and 2021 for the determination of physical and chemical properties such as permanganate index, chemical oxygen demand, biochemical oxygen demand (BOD5) and so on. By using the single factor pollution index method and the Nemerow pollution index method, the water quality of three monitoring sections and the whole Lugu Lake was assessed, and the temporal and spatial changes of water quality were analyzed. The findings demonstrate that Lugu Lake's overall water quality is excellent, and that it has not altered significantly in three years.The results of evaluating the water quality by the single factor pollution index method show that, in the past three years, the water quality of the three monitoring sections and the whole of Lugu Lake is Category I, which belongs to no pollution, and the measured indicators all meet the water quality standard of Category I. It can be seen from the evaluation results of the Nemerow index method that the water quality pollution index of Lugu Lake is between 0.22 and 0.34 in the past three years and the water quality evaluation of Changdao Bay, Lake center, Zhaojia Bay and the whole are Category I standards in 2019, 2020 and 2021. In terms of time changes, the water quality of Lugu Lake has remained stable between 2019 and 2021, and the water quality has been good. From the perspective of spatial changes, in 2019 and 2020, the water quality in Lake center is better than the monitoring sections of Changdao Bay and Zhaojia Bay.


Assuntos
Lagos , Poluentes Químicos da Água , China , Monitoramento Ambiental/métodos , Lagos/química , Poluentes Químicos da Água/análise , Poluição da Água/análise , Qualidade da Água
13.
Artigo em Chinês | MEDLINE | ID: mdl-35959580

RESUMO

Objective:To explore the application value of video head impulse test(vHIT), caloric test(CT) and the dizziness handicap inventory(DHI) in the diagnosis of acoustic neuroma(AN), to analyze the correlation between vHIT and CT, and to determine the correlationsof tumor size, vHIT, CT and DHI score. Methods:The clinical data of 24 patients with AN who underwent surgery in our department from January 2019 to January 2022 were analyzed retrospectively, including craniocerebral MRI, vHIT, caloric test and DHI score. All the data were statistically analyzed by GraphPadPrism9.0. Results:There was a significant negative correlation between the UW value of CT and the vestibular eye reflex gain of vHIT(P<0.01, r=-0.62). The tumor size was significantly correlated with the increase of UW value of CT(P<0.01, r=0.69), and with the decrease of vestibulo-ocular reflex gain of vHIT(P<0.01, r=-0.53). The average Dizziness Handicap Inventory score was 8.9±16.2, which was not correlated with tumor size(P>0.05). Conclusion:Both vHIT and CT can effectively evaluate the vestibular function of patients with AN(and they are complementary), and they are related to the size of the tumor and have certain value in the diagnosis of acoustic neuroma.

14.
Materials (Basel) ; 15(15)2022 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-35955249

RESUMO

As a nano-carbon material with excellent properties, Graphene oxide (GO) has been widely used in cement-based materials, and the negative effect of paste workability caused by GO agglomeration has also been widely concerning. In this study, a polycarboxylate-silane modified graphene oxide composite (PSG) was prepared by coupling polycarboxylate molecules to the surface of graphene oxide (GO) via a reaction with vinyl triethoxysilane. The effects of GO and PSG on the cement paste and the mechanisms underpinning these effects were investigated using fluidity and rheological parameter measurements, and ion concentration and zeta potential analyses. It was found that, in the aqueous phase of the paste, the polycarboxylate molecular chains on the surface of the PSG complexed with calcium ions (Ca2+), thereby preventing Ca2+ from bridging the GO sheets, and thus stabilizing the surface potential and the electrostatic repulsion. This prevented the PSG from forming an agglomerate structure such as that formed by GO under the same conditions, thereby substantially enhancing workability of paste with nano-carbon material. This study provides some new foundations and ideas for the further application of graphene oxide materials in cement-based materials.

15.
Exp Ther Med ; 24(2): 538, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35837040

RESUMO

[This retracts the article DOI: 10.3892/etm.2019.7713.].

16.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 38(8): 685-691, 2022 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-35851081

RESUMO

Objective To explore the effect of down-regulation of high mobility group box 1 (HMGB1) expression on inflammatory response and epithelial mesenchymal transition (EMT) in the lung tissue of mice with chronic obstructive pulmonary disease (COPD) and its related mechanism. Methods The COPD model was induced by cigarette smoking, and HMGB1 expression in lung tissue of mice was down-regulated by small interfering RNA (siRNA). The mice were divided into negative control group (NC group), COPD group, si-NC intervention COPD group, si-HMGB1 intervention COPD group, and tiotropium bromide intervention COPD group (positive control group). After 4 weeks of cigarette smoking induction, the general condition of mice were observed, and the body mass changes of each group were recorded every week. HE staining was used to observe the pathological changes of lung tissue in each group. The expression of HMGB1 mRNA and protein in lung tissues of mice weredetected by real time quantitative PCR and Western blot analysis. The BALF of mice in each group was collected, and the levels of IL-6, TNF-α, IL-1ß and TGF-ß1 in BALF were detected by ELISA. The expressions of E-cadherin and α-SMA in lung tissues of mice were observed by immunohistochemical staining. The expression of RAGE, TLR4, TGF-ß1 and the phosphorylation of NF-κB p65 in lung tissues were detected by Western blot analysis. Results COPD mouse model induced by cigarette smoking was successfully established. Compared with COPD group, down-regulation of HMGB1 expression in lung tissue significantly improved the general vital signs of mice, promoted the increase of body mass, and improved the pathological damage of lung tissue in mice. Compared with the control group, HMGB1 mRNA and protein expression levels increased significantly in COPD group, COPD combined with si-NC group and COPD combined with tiotropium group, while no significant HMGB1 expression was detected in COPD combined with si-HMGB1 group. Compared with the control group, the secretion levels of IL-6, TNF-α, IL-1ß, TGF-ß1 in BALF, the expression levels of α-SMA, RAGE, TLR4, TGF-ß1 and the phosphorylation level of NF-κB p65 in lung tissues were significantly increased in COPD model group, while the expression level of E-cadherin significantly decreased. Compared with the COPD group or the COPD combined with si-NC group, the changes of the above indexes in the lung tissue of mice in the COPD combined with si-HMGB1 group and the COPD combined with tiotropium group dropped markedly, and no significant difference between the latter two groups was found. Conclusion Downregulation of HMGB1 expression in lung tissue of mice can reduce pulmonary inflammatory response and EMT by inhibiting NF-κB pathway, thus ameliorating the progression of cigarette smoke-induced COPD disease.


Assuntos
Proteína HMGB1/metabolismo , Doença Pulmonar Obstrutiva Crônica , Animais , Caderinas/metabolismo , Regulação para Baixo , Transição Epitelial-Mesenquimal , Proteína HMGB1/genética , Interleucina-6/metabolismo , Camundongos , NF-kappa B/metabolismo , Doença Pulmonar Obstrutiva Crônica/genética , RNA Mensageiro , Brometo de Tiotrópio , Receptor 4 Toll-Like/genética , Receptor 4 Toll-Like/metabolismo , Fator de Crescimento Transformador beta1/metabolismo , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo
17.
J Integr Plant Biol ; 2022 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-35894630

RESUMO

Plants possess two cryptochrome photoreceptors, cryptochrome 1 (CRY1) and cryptochrome 2 (CRY2), that mediate overlapping and distinct physiological responses. Both CRY1 and CRY2 undergo blue light-induced phosphorylation, but the molecular details of CRY1 phosphorylation remain unclear. Here we identify 19 in vivo phosphorylation sites in CRY1 by mass spectrometry and systematically analyze the physiological and photobiochemical activities of CRY1 variants with phosphosite substitutions. We demonstrate that nonphosphorylatable CRY1 variants have impaired phosphorylation, degradation, and physiological functions, whereas phosphomimetic variants mimic the physiological functions of phosphorylated CRY1 to constitutively inhibit hypocotyl elongation. We further demonstrate that phosphomimetic CRY1 variants exhibit enhanced interaction with the E3 ubiquitin ligase COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC 1). This finding is consistent with the hypothesis that phosphorylation of CRY1 is required for COP1-dependent signaling and regulation of CRY1. We also determine that PHOTOREGULATORY PROTEIN KINASEs (PPKs) phosphorylate CRY1 in a blue light-dependent manner and that this phosphorylation is critical for CRY1 signaling and regulation. These results indicate that, similar to CRY2, blue light-dependent phosphorylation of CRY1 determines its photosensitivity. This article is protected by copyright. All rights reserved.

18.
Nanoscale ; 14(28): 10118-10124, 2022 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-35792617

RESUMO

As a potential oxygen evolution reaction (OER) catalyst, Co-based perovskites have received intensive attention. However, Sr readily accumulates on their surface, and makes them inert toward the OER. Herein, we propose a simple but versatile electrochemical reduction method to reconstruct the active surface of Co-based perovskites within a few seconds. By this method, Sr rapidly precipitates from Co-based perovskites, accompanied by the introduction of Sr and oxygen vacancies. After reconstruction, the electrochemical active surface areas of Co-based perovskites greatly increase, and the OER overpotential of the optimized SrNb0.1Co0.7Fe0.2O3-δ (ER-SNCF-20s) reaches 278 mV at 10 mA cm-2. This can be explained by the decrease of overpotentials at the rate-determining step. Using ER-SNCF-20s, the splitting voltage of alkaline natural seawater can reach 1.56 V at 10 mA cm-2, and remains steady for 300 h. This effort offers a feasible method for reconstructing the active surface of Co-based perovskites.

19.
ACS Sens ; 7(7): 1847-1854, 2022 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-35834210

RESUMO

The static labels presently prevalent on the food market are confronted with challenges due to the assumption that a food product only undergoes a limited range of predefined conditions, which cause elevated safety risks or waste of perishable food products. Hence, integrated systems for measuring food freshness in real time have been developed for improving the reliability, safety, and sustainability of the food supply. However, these systems are limited by poor sensitivity and accuracy. Here, a metal-organic framework mixed-matrix membrane and deep learning technology were combined to tackle these challenges. UiO-66-OH and polyvinyl alcohol were impregnated with six chromogenic indicators to prepare sensor array composites. The sensors underwent color changes after being exposed to ammonia at different pH values. The limit of detection of 80 ppm for trimethylamine was obtained, which was practically acceptable in the food industry. Four state-of-the-art deep convolutional neural networks were applied to recognize the color change, endowing it with high-accuracy freshness estimation. The simulation test for chicken freshness estimation achieved accuracy up to 98.95% by the WISeR-50 algorithm. Moreover, 3D printing was applied to create a mold for possible scale-up production, and a portable food freshness detector platform was conceptually built. This approach has the potential to advance integrated and real-time food freshness estimation.


Assuntos
Aprendizado Profundo , Estruturas Metalorgânicas , Ácidos Ftálicos , Reprodutibilidade dos Testes
20.
STAR Protoc ; 3(3): 101566, 2022 09 16.
Artigo em Inglês | MEDLINE | ID: mdl-35874472

RESUMO

3' UTR alternative polyadenylation (APA) quantitative trait loci (3'aQTL) can explain approximately 16.1% of trait-associated non-coding variants and is largely distinct from other molecular QTLs. Here, we describe a bioinformatic protocol for identifying 3'aQTLs through standard RNA-seq and matched genomic data. This protocol allows users to analyze dynamic APA events, identify common genetic variants associated with differential 3' UTR usage, and predict the potential causal variants that affect APA. For complete details on the use and execution of this protocol, please refer to Li et al. (2021).


Assuntos
Poliadenilação , Transcriptoma , Regiões 3' não Traduzidas/genética , Genômica , Poliadenilação/genética , Locos de Características Quantitativas/genética , Transcriptoma/genética
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