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1.
Artigo em Inglês | MEDLINE | ID: mdl-33428570

RESUMO

Shadow detection in general photos is a nontrivial problem, due to the complexity of the real world. Though recent shadow detectors have already achieved remarkable performance on various benchmark data, their performance is still limited for general real-world situations. In this work, we collected shadow images for multiple scenarios and compiled a new dataset of 10,500 shadow images, each with labeled ground-truth mask, for supporting shadow detection in the complex world. Our dataset covers a rich variety of scene categories, with diverse shadow sizes, locations, contrasts, and types. Further, we comprehensively analyze the complexity of the dataset, present a fast shadow detection network with a detail enhancement module to harvest shadow details, and demonstrate the effectiveness of our method to detect shadows in general situations.

2.
Phytother Res ; 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33474783

RESUMO

The Shen Yuan prescription (SY) comprises Panax ginseng (GT) and Polygala tenuifolia (YT), elicited superior antidepressant activity compared with that of GT or YT alone. The aim of this paper is to elucidate the effects of SY treatment on chronic social defeat stress (CSDS)-induced depression-like symptoms and the related mechanism. Our results indicated that SY treatment reverses the depressive-like behaviors induced by CSDS as measured by the social interaction test, sucrose preference test, forced swim test, and tail suspension test. SY decreased the serum levels of CORT and increased hippocampal neurotransmitters (5-HT, DA, and NE) in CSDS mice. Meanwhile, SY upregulated the brain-derived neurotrophic factor (BDNF) signaling pathway and reversed the decreased hippocampal neurogenesis caused by CSDS. In addition, we found that the TrkB antagonist K252a fully blocked the SY effects on behavioral improvement and eliminated the promoting effects of SY on hippocampal neurogenesis and BDNF-TrkB signaling (including the downstream ERK and Akt pathways) activation, thus further demonstrating that BDNF-TrkB signaling was necessary for the SY effects. In conclusion, our study showed that SY acted as an antidepressant in mice exhibiting CSDS-induced depression-like symptoms, and its effect was facilitated by promoting hippocampal neurogenesis and BDNF signaling pathway activation.

3.
Artigo em Inglês | MEDLINE | ID: mdl-33476269

RESUMO

Existing traditional and ConvNet-based methods for light field depth estimation mainly work on the narrow-baseline scenario. This paper explores the feasibility and capability of ConvNets to estimate depth in another promising scenario: wide-baseline light fields. Due to the deficiency of training samples, a large-scale and diverse synthetic wide-baseline dataset with labelled data is introduced for depth prediction tasks. Considering the practical goal for real-world applications, we design an end-to-end trained lightweight convolutional network to infer depths from light fields, called LLF-Net. The proposed LLF-Net is built by incorporating a cost volume which allows variable angular light field inputs and an attention module that enables to recover details at occlusion areas. Evaluations are made on the synthetic and real-world wide-baseline light fields, and experimental results show that the proposed network achieves the best performance when compared to recent state-of-the-art methods. We also evaluate our LLF-Net on narrow-baseline datasets, and it consequently improves the performance of previous methods.

4.
Artigo em Inglês | MEDLINE | ID: mdl-33462648

RESUMO

To understand the health impact represented by exposure to current atmospheric pollution in China, an environmental health indicators (EHIs) system of atmospheric pollution was established. The EHIs were based on comprehensive consideration of environment, population, economy and diseases associated with atmospheric pollution. An EHIs evaluation system of atmospheric pollution, based on corresponding EHIs data collection and weighting coefficients determined using principal component analysis, was applied to major provinces and regions in China to evaluate the environmental health status. Results showed that the EHIs of atmospheric pollution in Central and East China were low, indicating a serious environmental health condition. Prevention and management of atmospheric pollution in these regions should be strengthened and protective measures taken to improve human health. Compared with other methods, the EHIs evaluation system was more intuitive, which facilitated users to identify the environmental health status and provided support for health management and pollution prevention.

5.
Virus Res ; : 198307, 2021 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-33476695

RESUMO

Bats carry diverse severe acute respiratory syndrome-related coronaviruses (SARSr-CoVs). The possible interspecies transmission of SARSr-CoVs from bats to humans has caused two severe CoV pandemics, the SARS pandemic in 2003 and the recent COVID-19 pandemic. The receptor utilization of SARSr-CoV plays the key role in viral infection in determining the host range and the interspecies transmission ability of the virus. Both the SARS-CoV and SARS-CoV-2 use angiotensin-converting enzyme 2 (ACE2) as their receptor. Previous studies showed that WIV1 strain, the first living coronavirus isolated from bat using ACE2 as its receptor, is the prototype of SARS-CoV. The receptor-binding domain (RBD) in the spike protein (S) of SARS-CoV and WIV1 is responsible for ACE2 binding and medicates the viral entry. Comparing to human SARS-CoV, the bat WIV1 has three distinct amino acid residues (442, 472, and 487) in RBD. This study aimed at exploring whether these three residues could alter the receptor utilization of SARSr-CoVs. We replaced the three residues in SARS-CoV (BJ01 strain) S with their counterparts in WIV1 S, and then evaluated the change of their utilization of bat, civet, and human ACE2s using a lentiviral pseudovirus infection system. To further validate the S-ACE2 interactions, the binding affinity between the RBDs of these S proteins and different ACE2s were verified by flow cytometry. The results showed that the single amino acid substitution Y442S in the RBD of the SARS-CoV BJ01 spike protein enhanced its utilization of bat ACE2 and its binding affinity to bat ACE2. On the contrary, the reverse substitution in WIV1 S (S442Y) significantly attenuated the pseudovirus utilization of bat, civet and human ACE2s for cell entry, and the binding affinity was also decreased notably. These results suggest that the S442 is critical for WIV1 adapting in bats as its natural host. These findings will enhance our understanding of host adaptations and cross-species infections of coronaviruses, contributing to the prediction and prevention of coronavirus epidemics.

6.
J Hazard Mater ; 402: 123564, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33254743

RESUMO

Surfactant-enhanced air sparging (SEAS) is an effective remediation technique for VOCs-contaminated soil. In this study, three types of tests are performed to investigate the effects of the surfactant injection position on the airflow pattern, contaminant removal efficiency, and airflow path control. The three tests are conventional air sparging (CAS), entire SEAS (ESEAS), where the surfactant is incorporated into the entire contaminated soil, and local SEAS (LSEAS), where the surfactant is injected locally at different positions. With increasing distance between the injection position and the central axis, the LSEAS test results approach the results measured in the CAS test. When the surfactant is injected directly at the central axis, a high contaminant removal rate of 89% is obtained, which is even higher than that obtained for the ESEAS test. As the injection position moves away from the central axis, the removal rate decreases. Furthermore, when the injection position is close to the sparging point, the surfactant can successfully control the airflow path. Based on the test results, a critical distance between the surfactant injection position and sparging point exists where high remediation efficiency can be achieved. This optimal surfactant injection position is specific to each contamination site.

7.
Spectrochim Acta A Mol Biomol Spectrosc ; 248: 119252, 2021 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-33316655

RESUMO

A key issue for constructing optical and redox-active receptors is how to conjugate a specific sensing kernel with a multi-signal-responsive system to carry out multi-feature analysis. Mercury is considered to be highly toxic to human health and ecological security. In this work, we present a novel near-infrared optical and redox-active receptor that can sense Hg2+ at ppb level in aqueous media via multi-model monitors with a low detection limit of 8.4 × 10-9 M (1.68 ppb). This receptor features a visible detection, 'off-on' fluorescence response, and efficient electrochemistry assessment, as well as pH-insensitivity to Hg2+ with high sensitivity. In view of its marked near-infrared emission and fluorescence enhancement, we successfully applied this receptor to visualize Hg2+ in live cells. Furthermore, a possible sensing model was established and rationalized with theoretical studies.

8.
Micromachines (Basel) ; 11(12)2020 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-33255917

RESUMO

Cell separation plays an important role in the fields of analytical chemistry and biomedicine. To solve the blockage problem and improve the separation throughput in the traditional microstructure filtration-based separation approach, a continuous cell separation and collection approach via micropost array railing on a microfilter and negative dielectrophoresis combined chip is proposed. By tilting the micropost array at a certain angle, microparticles or cells enter the collection area under micropost array railing. The effects of the inclination angle of the micropost array and the electrode distance on the microparticle collection efficiency were investigated. Based on the optimized microfluidic chip structure, 37- and 16.3-µm particles were collected with 85% and 89% efficiencies, respectively. Additionally, algal cells were separated and collected by using the optimized microchip. The chip also had good separation and collection effects on biological samples, which effectively solved the blockage problem and improved the separation throughput, laying a foundation for subsequent microstructure filtration separation-based research and application.

9.
Data Brief ; 33: 106526, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33304948

RESUMO

Atropine, a non-selective muscarinic antagonist, is known to slow down myopia progression in human adolescents and in several animal models. However, its underlying molecular mechanism is unclear. The present work built a monocular form-deprivation myopia (FDM) guinea pig model, using facemasks as well as atropine treatment on FDM eyes for 2 and 4 weeks. Retinal protein changes in response to the FDM and effects of topical administration of atropine were screened for the two periods using fractionated isobaric tags for a relative and absolute quantification (iTRAQ) approach coupled with nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) (n=24, 48 eyes). Retinal tissues from another cohort receiving 4-weeks FDM with atropine treatment (n=12, 24 eyes) with more significant changes were subjected to sequential window acquisition of all theoretical mass spectra (SWATH-MS) proteomics for further protein target confirmation. A total of 1695 proteins (8875 peptides) and 5961 proteins (51871 peptides) were identified using iTRAQ and SWATH approaches, respectively. Using the Paragon algorithm in the ProteinPilotTM software, the three most significantly up-regulated and down-regulated proteins that were commonly found in both ITRAQ and SWATH experiments are presented. All raw data generated from the work were submitted and published in the Peptide Atlas public repository (http://www.peptideatlas.org/) for general release (Data ID PASS01507).

10.
Rice (N Y) ; 13(1): 80, 2020 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-33284383

RESUMO

BACKGROUND: As rice (Oryza sativa) is the staple food of more than half the world's population, rice production contributes greatly to global food security. Rice blast caused by the fungus Magnaporthe oryzae (M. oryzae) is a devastating disease that affects rice yields and grain quality, resulting in substantial economic losses annually. Because the fungus evolves rapidly, the resistance conferred by most the single blast-resistance genes is broken after a few years of intensive agricultural use. Therefore, effective resistance breeding in rice requires continual enrichment of the reservoir of resistance genes, alleles, or QTLs. Seed banks represent a rich source of genetic diversity; however, they have not been extensively used to identify novel genes and alleles. RESULTS: We carried out a large-scale screen for novel blast-resistance alleles in 1883 rice varieties from major rice-producing areas across China. Of these, 361 varieties showed at least moderate resistance to natural infection by rice blast at rice blast nurseries in Enshi and Yichang, Hubei Province. We used sequence-based allele mining to amplify and sequence the allelic variants of the major rice blast-resistance genes at the Pi2/Pi9 locus of chromosome 6 from the 361 blast-resistant varieties, and the full-length coding region of this gene could be amplified from 107 varieties. Thirteen novel Pi9 alleles (named Pi9-Type1 to Pi9-Type13) were identified in these 107 varieties based on comparison to the Pi9 referenced sequence. Based on the sequencing results, the Pi2/Pi9 locus of the 107 varieties was divided into 15 genotypes (including three different genotypes of Pi9-Type5). Fifteen varieties, each representing one genotype, were evaluated for resistance to 34 M. oryzae isolates. The alleles from seven varieties with the highest resistance and widest resistance spectra were selected for transformation into the susceptible variety J23B to construct near-isogenic lines (NILs). These NILs showed resistance in a field test in Enshi and Yichang, indicating that the seven novel rice blast-resistance tandem-repeat regions at the Pi2/Pi9 locus of chromosome 6 could potentially serve as a genetic resource for molecular breeding of resistance to rice blast. CONCLUSIONS: The thirteen novel Pi9 alleles identified in this study expand the list of available of blast-resistance alleles. Seven tandem-repeat regions of the Pi2/Pi9 locus from different donors were characterized as broad-spectrum rice blast-resistance fragments; these donors enrich the genetic resources available for rice blast-resistance breeding programs.

11.
Crit Rev Food Sci Nutr ; : 1-18, 2020 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-33305589

RESUMO

The aim of this systematic review and meta-analysis was to analyze data from randomized controlled trials (RCTs) assessing the effects of oleic acid (OA) supplementation on blood inflammatory markers in adults. PubMed, EMBASE and Cochrane Library databases were systematically searched from 1950 to 2019, with adults and a minimum intervention duration of 4 weeks. The effect size was estimated, adopting standardized mean difference (SMD) and 95% confidence interval (CI). Of the 719 identified studies, thirty-one RCTs involving 1634 subjects were eligible. The results of this study revealed that increasing OA supplementation significantly reduced C-reactive protein (CRP) (SMD: -0.11, 95% CI: -0.21, -0.01, P = 0.038). However, dietary OA consumption did not significantly affect tumor necrosis factor (TNF) (SMD: -0.05, 95% CI: -0.19, 0.10, P = 0.534), interleukin 6 (IL-6) (SMD: 0.01, 95% CI: -0.10, 0.13, P = 0.849), fibrinogen (SMD: 0.08, 95% CI: -0.16, 0.31, P = 0.520), plasminogen activator inhibitor type 1 (PAI-1) activity (SMD: -0.11, 95% CI: -0.34, 0.12, P = 0.355), soluble intercellular adhesion molecule-1 (sICAM-1) (SMD: -0.06, 95% CI: -0.26, 0.13, P = 0.595) or soluble vascular cell adhesion molecule-1 (sVCAM-1) (SMD: -0.04, 95% CI: -0.27, 0.18, P = 0.701). Overall, the meta-analysis demonstrated that dietary OA supplementation significantly reduced CRP, yet did not affect other inflammatory markers including TNF, IL-6, fibrinogen, PAI-1 activity, sICAM-1or sVCAM-1.

12.
Aging (Albany NY) ; 122020 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-33291081

RESUMO

Genome-wide association studies have revealed that multiple single-nucleotide polymorphisms in the intergenic region between estrogen receptor 1 and coiled-coil domain containing 170 (CCDC170) are associated with breast cancer risk. We performed microarray and bioinformatics analyses to identify genes that were induced upon CCDC170 overexpression, and confirmed our findings by evaluating paraffin-embedded breast cancer tissues and conducting cellular assays. In CCDC170-overexpressing MCF7 breast cancer cells, microarray analyses revealed that inositol-requiring enzyme 1 (IRE1) was the most elevated gene in enriched pathways. In breast cancer tissues, IRE1 expression correlated positively with CCDC170 and X-box binding protein 1 expression at both the mRNA and protein levels. In a survival analysis, patients with higher CCDC170 levels exhibited better disease-free survival. Western blotting indicated that overexpressing CCDC170 in MCF7 cells increased protein levels of IRE1α, estrogen receptor α and X-box binding protein 1, while silencing CCDC170 reduced them. CCDC170 overexpression promoted apoptosis in MCF7 cells, and this effect was more obvious under endoplasmic reticulum stress. MCF7 cells overexpressing CCDC170 were more sensitive to paclitaxel. Our study showed that higher CCDC170 expression is associated with a better prognosis in breast cancer patients and that CCDC170 may promote apoptosis through the IRE1α pathway.

13.
ChemSusChem ; 2020 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-33274593

RESUMO

A stable solid acid catalyst, SCPR140-1, was synthesized from chloromethyl polystyrene resin (CPR) and used for catalytic pretreatment of corncob in aqueous solution. Under the optimized pretreatment condition, 73.07% of xylose was directly obtained and then the enzymatic digestibility of treated residue reached up to 94.65%, indicating that the SCPR140-1 has high selectivity for xylose production and effectively deconstructs the structure of corncob. The -CH2Cl group of CPR was substituted by -SO3H through the sulfonation and the -SO3H was stable bond on the catalyst during the pretreatment process. Compared with other similar reports, the SCPR140-1 is not only synthesized through a simpler process but also has a more stable catalytic activity during the multiple recycling.

14.
Technol Cancer Res Treat ; 19: 1533033820962114, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33267716

RESUMO

OBJECTIVE: To explore the inhibitory and synergistic effects of 5-fluorouracil and curcumin on Hep-2 laryngeal cancer cells and clarify the effect of mesoporous silica nanoparticles as drug carriers. METHODS: The inhibitory effects of 5-fluorouracil and curcumin on Hep-2 cells were detected using the CCK-8 assay. CompuSyn was used to calculate the synergistic effect of the 2 drugs. Flow cytometry was used to detect apoptosis and cell cycle arrest induced by 5-fluorouracil and curcumin. The drugs were loaded into mesoporous nanoparticles. Western blotting was used to detect the expression of related proteins after treatment. The growth of subcutaneous tumors in BALB/c nude after the intraperitoneal injection with drug-loaded mesoporous silica nanoparticles was recorded. RESULTS: 5-Fluorouracil and curcumin synergistically induced apoptosis and cell cycle arrest in Hep-2 cells. Mesoporous silica nanoparticles as drug carriers enhanced the therapeutic effects of 5-fluorouracil and curcumin. CONCLUSIONS: Mesoporous silica nanoparticles are expected to be effective drug carriers that enhance the synergistic effects of 5-fluorouracil and curcumin on laryngeal cancer.

15.
Artigo em Inglês | MEDLINE | ID: mdl-33382073

RESUMO

Myocardial injury is still a serious condition damaging the public health. Clinically, myocardial injury often leads to cardiac dysfunction and, in severe cases, death. Reperfusion of the ischemic myocardial tissues can minimize acute myocardial infarction (AMI)-induced damage. MicroRNAs are commonly recognized in diverse diseases and are often involved in the development of myocardial ischemia/reperfusion injury. However, the role of miR-431 remains unclear in myocardial injury. In this study, we investigated the underlying mechanisms of miR-431 in the cell apoptosis and autophagy of human cardiomyocytes in hypoxia/reoxygenation (H/R). H/R treatment reduced cell viability, promoted cell apoptotic rate, and down-regulated the expression of miR-431 in human cardiomyocytes. The down-regulation of miR-431 by its inhibitor reduced cell viability and induced cell apoptosis in the human cardiomyocytes. Moreover, miR-431 down-regulated the expression of autophagy-related 3 (ATG3) via targeting the 3'-untranslated region of ATG3. Up-regulated expression of ATG3 by pcDNA3.1-ATG3 reversed the protective role of the overexpression of miR-431 on cell viability and cell apoptosis in H/R-treated human cardiomyocytes. More importantly, H/R treatments promoted autophagy in the human cardiomyocytes, and this effect was greatly alleviated via miR-431-mimic transfection. Our results suggested that miR-431 overexpression attenuated the H/R-induced myocardial damage at least partly through regulating the expression of ATG3.

17.
Mol Cell Endocrinol ; 523: 111135, 2020 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-33359761

RESUMO

Elevated lipogenesis is an important metabolic hallmark of rapidly proliferating tumor such as endometrial carcinoma (EC). The sterol regulatory element-binding protein 1 (SREBP1) is a master regulator of lipogenesis and involved in EC proliferation. BF175 is a novel chemical inhibitor of SREBP pathway, and has shown potent anti-lipogenic effects. However, the effect of BF175 on EC cells are yet to be determined. In the present study, we found that BF175 decreased cell viability, colony formation and migratory capacity, inducing autophagy and mitochondrial related apoptosis in EC cell line AN3CA. Z-VAD-FMK partially attenuated the effect of BF175 on AN3CA. In addition, BF175 significantly downregulated SREBPs and their downstream genes. The levels of free fatty acids and total cholesterol were also inhibited. Microarray analysis suggested BF175 treatment obviously affected lipid metabolic pathways in EC. Taken together, we validated BF175 exhibited anti-tumor activity by targeting SREBP-dependent lipogenesis and inducing apoptosis which mitochondrial pathway involved in, suggesting that it's potential as a novel therapeutic reagent for EC.

18.
Exp Ther Med ; 20(6): 224, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33193838

RESUMO

The costimulatory receptors CD27 and CD28 have pivotal and non-redundant roles in the activation and differentiation of γδ T-cells. However, the roles of CD27 and CD28 on γδ T-cells in allergic rhinitis (AR) have remained elusive. The aim of the present study was to investigate the expression of CD27 and CD28 on γδ T cells in patients with AR. Peripheral blood mononuclear cells from 14 patients with AR and 12 healthy subjects were isolated and analyzed by flow cytometry to determine the percentage of γδ T cells and regulatory T cells (Tregs), and the expression of IFN-γ, IL-17A, CD27 and CD28 on γδ T cells. The correlations between the expression of CD27 and CD28, and the percentages of IFN-γ+ and IL-17A+ γδ T-cell subsets and Tregs in AR were analyzed. It was observed that the percentages of γδ T cells, and the IL-17A+, CD27-CD28+ and CD27-CD28- γδ T-cell subsets were significantly increased, while the percentages of Tregs and IFN-γ+ and CD27+CD28+ γδ T-cell subsets were significantly decreased in AR. Of note, the percentage of CD27+CD28+ γδ T-cell subsets was positively correlated with that of the IFN-γ+ γδ T-cell subset and the percentage of the CD27-CD28+ γδ T-cell subset was positively correlated with that of the IL-17A+ γδ T-cell subset. Furthermore, the percentages of γδ T cells and the CD27-CD28+ γδ T-cell subset were both negatively correlated with that of Tregs. Therefore, the results of the present study indicate that CD27 and CD28 may be the key signals for activation of different γδ T-cell subsets and may contribute to the immune regulatory function of γδ T cells in the peripheral blood of patients with AR.

19.
Am J Transl Res ; 12(10): 6235-6249, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33194026

RESUMO

Mounting lines of evidence indicated that the "colony stimulating factor-1 (CSF-1)/tumor-associated macrophage (TAM)" signature plays an important role in the progression, invasion and metastasis of multiple tumors. However, the potential role of CSF-1/TAM in oral squamous cell carcinoma (OSCC) remains largely unknown. In the present study, the expression of CSF-1 from 99 OSCC specimens and its correlation with clinicopathological features and patient outcomes were investigated. Meanwhile, the correlation between CSF-1 expression and TAM infiltration was also explored. To investigate the potential effect of CSF-1 on tumor growth, nude mice were subcutaneously injected with Cal27 cell line and a small molecule inhibitor of CSF-1 (BZL945). The results showed that the high expression rate of CSF-1 (52%) was found in OSCC, and the upregulation of CSF-1 was closely correlated with lymph node metastasis and clinical stage. Additionally, there was a positive correlation between a high CSF-1 level and elevated TAM infiltration. The xenograft model study showed that CSF-1 signal blockade inhibited tumor growth, with a significant synchronous decrease in CSF-1 expression and TAM infiltration. Overall, our findings indicated that CSF-1 plays a crucial role in TAMs-mediated OSCC tumor progression and invasion. The "CSF-1/TAM" signaling axis may serve as a prospective target for anti-tumor therapy of OSCC.

20.
Opt Express ; 28(23): 34378-34389, 2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33182909

RESUMO

In this paper, a curved composite hologram (CCH) generation method with suppressed speckle noise is proposed. The recorded 3D object is considered as many layers with different depths and the planar hologram (PH) for each layer is generated accordingly. Then the PH is transformed to curved hologram (CH). The CH of the recorded 3D object can be generated by superposing the CHs for all the layers. Also, the linear phase factor is superposed to the CH of the object. For different objects, the bending angle and linear phase factor of the hologram are different. The CCH is generated by superimposing the CHs of different objects. Finally, the CCH is encoded by using the error diffusion method and optimized by superposing of the digital lens. When the CCH is reproduced from different angles, the reconstructed images with suppressed speckle noise can be displayed. The experimental results verify the feasibility of the proposed method.

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