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1.
Bioengineered ; 12(1): 7694-7703, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34608825

RESUMO

The aim of this study was to explore the effects and action mechanism of Zhike Pingchuan Granule in human bronchial epithelial cells induced by IL-6 or the supernatant of M2. Upon IL-6 stimulation at different doses, Cell Counting Kit-8 (CCK8) assay and flow cytometry were, respectively, utilized to detect the cell viability and apoptosis levels of 16-HBE cells. ELISA and Western blot were, respectively, used to analyze the inflammatory markers and JAK2/STAT3 signals. Immunofluorescence assay was performed to identify M0 and M2 cells. As shown in results, ZKPC perturbed the expression of IL-6 inducible genes important for apoptosis, oxidative and inflammatory response, which was enhanced by JAK2 inhibitor. Besides the inhibitory effects on the phosphorylation levels of JAK2/STAT3, ZKPC markedly increased cell viability and reduced apoptosis in human bronchial epithelial cells (16-HBE) cultured in the supernatant of M2 cells. Collectively, ZKPC could inhibit the IL-6-induced JAK/STAT3 signaling cascade, increase cell viability and decrease apoptosis induced by the supernatant of M2. A more comprehensive understanding of the action mechanism of ZKPC on JAK2/STAT3 signaling pathway in human bronchial epithelial cells induced by IL-6 or M2 supernatant will enable ZKPC development in the control of asthma.

2.
JAMA Netw Open ; 4(10): e2128217, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34609494

RESUMO

Importance: Few studies have examined the clinicopathological characteristics and prognoses of patients with hepatoid adenocarcinoma of the stomach (HAS). Objective: To explore the clinicopathological characteristics and prognoses of patients with HAS and develop a nomogram to predict overall survival (OS). Design, Setting, and Participants: This prognostic study involved a retrospective analysis of data from 315 patients who received a diagnosis of primary HAS between April 1, 2004, and December 31, 2019, at 14 centers in China. Main Outcomes and Measures: OS and prognostic factors. Patients were randomly assigned to a derivation cohort (n = 220) and a validation cohort (n = 95). A nomogram was developed based on independent prognostic factors identified through a multivariable Cox mixed-effects model. Results: Among 315 patients with HAS (mean [SD] age, 61.9 [10.2] years; 240 men [76.2%]), 137 patients had simple HAS (defined as the presence of histologically contained hepatoid differentiation areas only), and 178 patients had mixed HAS (defined as the presence of hepatoid differentiation areas plus common adenocarcinoma areas). Patients with simple HAS had a higher median preoperative α-fetoprotein level than those with mixed HAS (195.9 ng/mL vs 48.9 ng/mL, respectively; P < .001) and a higher rate of preoperative liver metastasis (23 of 137 patients [16.8%] vs 11 of 178 patients [6.2%]; P = .003). The 3-year OS rates of patients with simple vs mixed HAS were comparable (56.0% vs 60.0%; log-rank P = .98). A multivariable Cox analysis of the derivation cohort found that the presence of perineural invasion (hazard ratio [HR], 2.13; 95% CI, 1.27-3.55; P = .009), preoperative carcinoembryonic antigen levels of 5 ng/mL or greater (HR, 1.72; 95% CI, 1.08-2.74; P = .03), and pathological node category 3b (HR, 3.72; 95% CI, 1.34-10.32; P = .01) were independent risk factors for worse OS. Based on these factors, a nomogram to predict postoperative OS was developed. The concordance indices of the nomogram (derivation cohort: 0.72 [95% CI, 0.66-0.78]; validation cohort: 0.72 [95% CI, 0.63-0.81]; whole cohort: 0.71 [95% CI, 0.66-0.76]) were higher than those derived using the American Joint Committee on Cancer's AJCC Cancer Staging Manual (8th edition) pathological tumor-node-metastasis (pTNM) staging system (derivation cohort: 0.63 [95% CI, 0.57-0.69]; validation cohort: 0.65 [95% CI, 0.56-0.75]; whole cohort: 0.64 [95% CI, 0.59-0.69]) and those derived using a clinical model that included pTNM stage and receipt of adjuvant chemotherapy (derivation cohort: 0.64 [95% CI, 0.58-0.69]; validation cohort: 0.65 [95% CI, 0.56-0.75]; whole cohort: 0.64 [95% CI, 0.59-0.69]). Based on the nomogram cutoff of 10 points, the whole cohort was divided into high-risk and low-risk groups. The 3-year OS rate of patients in the high-risk group was significantly lower than that of patients in the low-risk group (29.7% vs 75.9%, respectively; log-rank P < .001), and the 3-year prognosis of high-risk and low-risk groups could be further distinguished into pTNM stage I to II (33.3% vs 80.2%; exact log-rank P = .15), stage III (34.3% vs 71.3%; log-rank P < .001), and stage IV (15.5% vs 70.3%; log-rank P = .009). Conclusions and Relevance: This study found that perineural invasion, preoperative carcinoembryonic antigen levels of 5 ng/mL or greater, and pathological node category 3b were independent risk factors associated with worse OS. An individualized nomogram was developed to predict OS among patients with HAS. This nomogram had good prognostic value and may be useful as a supplement to the current American Joint Committee on Cancer TNM staging system.

3.
J Am Chem Soc ; 143(41): 17059-17067, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34609874

RESUMO

The boundary between molecular and metallic gold nanoclusters is of special interest. The difficulty in obtaining atomically precise nanoclusters larger than 2 nm limits the determination of such a boundary. The synthesis and total structural determination of the largest all-alkynyl-protected gold nanocluster (Ph4P)6[Au156(C≡CR)60] (R = 4-CF3C6H4-) (Au156) are reported. It presents an ideal platform for studying the relationship between the structure and the metallic nature. Au156 has a rod shape with the length and width of the kernel being 2.38 and 2.04 nm, respectively. The cluster contains a concentric Au126 core structure (Au46@Au50@Au30) protected by 30 linear RC≡C-Au-C≡CR staple motifs. It is interesting that Au156 displays multiple excitonic peaks in the steady-state absorption spectrum (molecular) and pump-power-dependent excited-state dynamics as revealed in the transient absorption spectrum (metallic), which indicates that Au156 is a critical crossover cluster for the transition from molecular to metallic state. Au156 is the smallest-sized gold nanocluster showing metal-like electron dynamics, and it is recognized that the cluster shape is one of the important factors determining the molecular or metallic nature of a gold nanocluster.

4.
Photosynth Res ; 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34491493

RESUMO

The plant photosynthetic capacity determines the photosynthetic rates of the terrestrial biosphere. Timely approaches to obtain the spatiotemporal variations of the photosynthetic parameters are urgently needed to grasp the gas exchange rhythms of the terrestrial biosphere. While partial least squares regression (PLSR) is a promising way to predict the photosynthetic parameters maximum carboxylation rate (Vcmax) and maximum electron transport rate (Jmax) rapidly and non-destructively from hyperspectral data, the approach, however, faces a high risk of overfitting and remains a high hurdle for applications. In this study, we propose to incorporate proper band selection techniques for PLSR analysis to refine the goodness-of-fit (GoF) in estimating Vcmax and Jmax. Different band selection procedures coupled with different hyperspectral forms (reflectance, apparent absorption, as well as derivatives) were examined. Our results demonstrate that the GoFs of PLSR models could be greatly improved by combining proper band selection methods (especially the iterative stepwise elimination approach) rather than using full bands as commonly done with PLSR. The results also show that the 1st order derivative spectra had a balance between accuracy (R2 = 0.80 for Vcmax, and 0.94 for Jmax) and denoising (when a Gaussian noise was added to each leaf reflectance spectrum at each wavelength with a standard deviation of 1%) on retrieving photosynthetic parameters from hyperspectral data. Our results clearly illustrate the advantage of using the band selection approach for PLSR dimensionality reduction and model optimization, highlighting the superiority of using derivative spectra for Vcmax and Jmax estimations, which should provide valuable insights for retrieving photosynthetic parameters from hyperspectral remotely sensed data.

5.
J Hazard Mater ; 423(Pt A): 127081, 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34523481

RESUMO

Finding a low-cost and suitable adsorbent is still in urgent need for efficient decontamination of As(III) and As(V) elements from the polluted waters. A novel zirconium hydroxide nanoparticle encapsulated magnetic biochar composite (ZBC) derived from rice residue was synthesized for the adsorptive capture of As(III) and As(V) from aqueous solutions. The results revealed that ZBC showed an acceptable magnet separation ability and its surface was encapsulated with lots of hydrous zirconium oxide nanoparticles. Compared to As(III), the adsorption of As(V) onto ZBC was mainly dependent on the pH of the solution. The intraparticle diffusion model described the adsorption process. ZBC showed satisfactory adsorption performances to As(III) and As(V) with the highest adsorption quantity of 107.6 mg/g and 40.8 mg/g at pH 6.5 and 8.5, respectively. The adsorption of As(III) and As(V) on ZBC was almost impervious with the ionic strength while the presence of coexisting ions, especially phosphate, significantly affected the adsorption process. The processes of complexation reaction and electrostatic attraction contributed to the adsorption of As(III) and As(V) onto ZBC. ZBC prepared from kitchen rice residue was found to be a low cost environmentally friendly promising adsorbent with high removal capacity for As(III) and As(V) and could be recycled easily from contaminated waters.

6.
Artigo em Inglês | MEDLINE | ID: mdl-34546917

RESUMO

We are concerned with using user-tagged images to learn proper hashing functions for image retrieval. The benefits are two-fold: (1) we could obtain abundant training data for deep hashing models; (2) tagging data possesses richer semantic information which could help better characterize similarity relationships between images. However, tagging data suffers from noises, vagueness and incompleteness. Different from previous unsupervised or supervised hashing learning, we propose a novel weakly-supervised deep hashing framework which consists of two stages: weakly-supervised pre-training and supervised fine-tuning. The second stage is as usual. In the first stage, we propose two formulations Tag-basEd weakLy-supErvised Modally COoperative hashing Network (TelecomNet) and Generalized TelecomNet (GTelecomNet). Rather than performing supervision on tags, TelecomNet first learns an observed semantic embedding vector for each image from attached tags and then uses it to guide hashing learning. GTelecomNet introduces a novel semantic network to exploit more precise semantic information. By carefully designing the optimization problem, they can well leverage tagging information and image content for hashing learning. The framework is general and does not depend on specific deep hashing methods. Empirical results on real world datasets show that they significantly increase the performance of state-of-the-art deep hashing methods.

7.
Work ; 70(1): 199-207, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34511524

RESUMO

BACKGROUND: Return to work is an important process for many breast cancer survivors (BCSs) that acts as a positive step towards their reintegration into society. OBJECTIVES: This study examined whether work productivity loss due to presenteeism could predict the quality of life (QOL) of employed BCSs. METHODS: This study used a cross-sectional design. Seventy-five BCSs and seventy-five participants in the Non-Cancer Comparison (NCC) group were surveyed. The main outcome measures were productivity loss (as measured by the Work Limitations questionnaire) and quality of life (as measure by the European Organization for Research and Treatment Quality of Life questionnaire C30). Other measures included psychological distress (as measured by the Hospital Anxiety and Depression Scale) and cognitive limitation at work (as measured by the Cognitive Symptom Checklist). RESULTS: The BCS group had a lower summary score, a lower global health related score and greater work limitation in all domains than the NCC group. The productivity loss due to presenteeism of the BCS group was 8%. The multiple regression model shows that work productivity loss and level of job stress were the significant predictors of quality of life in the BCS group. CONCLUSION: These findings raise questions about the effects of level of job stress and work productivity loss on the QOL of BCSs. Longitudinal studies are needed to map these relationships.


Assuntos
Neoplasias da Mama , Sobreviventes de Câncer , Mama , Neoplasias da Mama/complicações , Estudos Transversais , Feminino , Humanos , Qualidade de Vida
8.
Nanotechnology ; 32(50)2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34547731

RESUMO

Adding conductive graphene into polyvinyliden1 fluoride (PVDF) is an effective method to improve the dielectric properties. However, the high conductivity and uniform distribution of graphene in PVDF matrix still meet challenges. In this work, electrochemical exfoliated graphite (EEG) with good conductivity and solution dispersion is used to prepare PVDF/EEG films. By this method no specific conditions are needed such as reduction or hot press. The dielectric properties and flexibility of PVDF/EEG films of different concentrations of EEG are investigated. Especially, when EEG content is 2.6 wt%, composite film has a high dielectric constant of 86 and a low dielectric loss of 0.9 (100 Hz). Simultaneously, the tensile strength of the film was up to 55.1 MPa. The preparation method is simple and convenient, and the obtained material has high dielectric constant and tensile strength. This method paves the way for the application of flexible electronic sensing equipment.

9.
J Alzheimers Dis ; 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34542069

RESUMO

BACKGROUND: Few studies have investigated the association between cognition and brain volume associated with cerebral small vessel disease (CSVD). OBJECTIVE: We investigated the association between cognition and brain volume and neuroimaging markers of CSVD in a community-dwelling population. METHODS: Participants (n = 993, age≥35 years) from the community-based Shunyi Study were included to investigate the association between neuroimaging markers and cognition cross-sectionally. Magnetic resonance imaging markers included brain volume measurements of the total cerebrum, white matter, gray matter, and CSVD imaging markers. Cognitive performance was assessed using neuropsychological tests of the Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), Fuld Object Memory, digit span, Trail Making Test (TMT)-A, and TMT-B. RESULTS: For brain volume measurement, subcortical white matter fraction was positively associated with MMSE score (ß= 0.034, p = 0.0062) and MoCA score (ß= 0.034, p = 0.0174), and negatively associated with TMT-A and TMT-B completion time (ß= -2.319, p = 0.0002; ß= -2.827, p = 0.0073, respectively). For evaluation of CSVD imaging markers, the presence of lacunes was positively associated with TMT-B completion time (ß= 17.241, p = 0.0028). CONCLUSION: In community-dwelling populations, reduced white matter volumes, as a consequence of aging and vascular damage, are associated with worse global cognition and executive function. Our findings provide potential insights into the correlation between cognition and CSVD-associated subcortical white matter injury.

10.
Sci Total Environ ; 803: 150029, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34525714

RESUMO

The effect of microplastics (MPs) on the biological treatment of organic waste has been extensively studied, but little is known about the influence of different MPs on composting humification and the fungal community. In this study, PE, PVC, and PHA MPs were individually mixed with cow dung and sawdust and then composted. The results showed that different MPs had various influences on humification, and the humic acid to fulvic acid ratio of all MP-added treatments (0.44-0.83) was lower than that of the control (0.91). During the composting process, Ascomycota (26.32-89.14%) and Basidiomycota (0.47-4.78%) are the dominant phyla in all treatments and all microplastics decreased the diversity and richness of the fungal community at the thermophilic stage of composting. Exposure to MPs had an obvious effect on the fungal community at the genus level, and the addition of PHA and PE MPs increased the relative abundance of phytopathogenic fungi. LEfSe and network analysis indicated that MPs reduced the number of biomarkers and led to a simpler and more unstable fungal community structure compared to the control. This study has important implications for assessing microplastic pollution and organic waste disposal.

12.
Physiol Plant ; 2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34549440

RESUMO

Water-saving and drought-resistant rice (WDR) is widely grown in central China in recent years. However, studies have not explored the interaction effect of WDR and irrigation regimes on drought-resistance capacities under severe drought at sensitive growth periods. A pot experiment was conducted using a WDR cultivar Hanyou73 (HY73) and traditional high-yielding and drought-sensitive cultivar Huiliangyou 898 (HLY898). Three irrigation regimes, including flooding irrigation (W1), mild wetting-drying alternation irrigation (W2), and severe wetting-drying alternation irrigation (W3), were applied before heading. At heading, severe drought with -50 KPa soil water potential was established for all treatments and cultivars. The findings showed that cultivar HY73 under W2 treatment had the highest yield, 1000-grain yield, filled grain, relative water content, and photosynthesis potential compared with the other combinations. The higher net photosynthetic rate (Pn ) was attributed to larger mesophyll conductance (gm ) in drought for cultivar HY73 under W2 treatment compared with that for cultivar HLY898 and the other water treatments. Enhanced photo-respiration rate may be an important photoprotection mechanism for achieving high Pn for cultivar HY73 coupled with W2 treatment than for other combinations in drought. The relative expression level of OsPIP1;1 gene was significantly down-regulated during drought in all cultivars and water regimes. But OsPIP1;2, OsPIP2;3, OsTIP2;2, and OsTIP3;1 genes were upregulated to alleviate the significant decrease in gs and gm under drought. These results suggest that WDR and mild wetting-drying alternation irrigation (W2) have significant interaction effects in improving photosynthetic production potential by maintaining higher gm under severe drought.

13.
Polymers (Basel) ; 13(16)2021 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-34451292

RESUMO

The cavity pressure in the injection molding process is closely related to the quality of the molded products, and is used for process monitoring and control, to upgrade the quality of the molded products. The experimental platform was built to carry out the cavity pressure experiment with a micro spline injection mold in the paper. The process parameters were changed, such as V/P switchover, mold temperature, melt temperature, packing pressure, and injection rate, in order to analyze the influence of the process parameters on the product weight. The peak cavity pressure and area under the pressure curve were the two attributes utilized in investigating the correlation between cavity pressure and part weight. The experimental results show that the later switchover allowed the injection to proceed longer and produce a heavier tensile specimen. By comparing different cavity pressure curves, the general shapes of the curves were able to indicate different types of shortage produced. When the V/P switchover position is 10 mm, the coefficient of determination (R2 value) of part weight, for the peak cavity pressure and area under the curve, were 0.7706 and 0.8565, respectively. This showed that the area under the curve appeared to be a better process and quality indicator than the peak cavity pressure.

14.
Adv Healthc Mater ; : e2101036, 2021 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-34414687

RESUMO

In the field of tumor imaging and therapy, the aggregation-caused quenching (ACQ) effect of fluorescent dyes at high concentration is a great challenge. In this regard, the aggregation-induced emission luminogens (AIEgens) show great potential, since AIEgens effectively overcome the ACQ effect and have better fluorescence quantum yield, photobleaching resistance, and photosensitivity. Polyethylene glycol (PEG)-polymer is the most commonly used carrier to prepare nanoparticles (NPs). The advantage of PEGylation is that it can greatly prolong the metabolic half-life and reduce immunogenicity and toxicity. Considering that the hydrophobicity of most AIEgens hinders their application in organisms, the use of PEG-polymer encapsulation is an effective strategy to overcome this obstacle. Importantly, bioactive functional groups can be modified on PEG-polymers to enhance the biological effect of NPs. The combination of powerful AIEgens and PEG-polymers provides a new strategy for tumor imaging and therapy, which is promising for clinical application.

15.
Adv Healthc Mater ; : e2100966, 2021 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-34363350

RESUMO

Gastric cancer is one of the most common malignant tumors, which remains as an obstacle to human health. Nowadays, targeted nanoparticles to gastric tumor tissues, provide new strategy for improved therapy but still remain challenging. The major hurdle of targeted therapeutic nanoparticles comes from the limited enrichment and poor selectivity of therapeutic agents in in situ tumor. Herein, a pH-sensitive targeted nano platform coloaded As2 O3 and human epidermal growth factor receptor-2 (HER2)-siRNA (AH RNPs) is developed to achieve targeting therapy in orthotopic gastric carcinoma. AH RNPs can effectively prevent the degradation of siRNA and overcome the poor solubility of As2 O3 . In vitro studies show that AH RNPs could achieve synergistic inhibition of growth and metastasis on SGC7901 cells. Surprisingly, AH RNPs not only target gastric subcutaneous tumor, but also target in situ tumor, and express loaded genes in in situ tumor. Moreover, AH RNPs show excellent antitumor effect in orthotopic gastric tumor model and the anticancer mechanism is related about inhibiting the activation of ERK signal and downregulating the expression of cxc chemokine receptor 4 (CXCR4), HER2, MMP2, and MMP9 protein. This study provides a multi-functional vector for precise targeting therapy of gastric cancer, which may serve as a potential clinical application for future gastric cancer.

16.
J Evid Based Med ; 14(3): 218-231, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34463038

RESUMO

In the past years, network meta-analysis (NMA) has been widely used among clinicians, guideline makers, and health technology assessment agencies and has played an important role in clinical decision-making and guideline development. To inform further development of NMAs, we conducted a bibliometric analysis to assess the current status of published NMA methodological studies, summarized the methodological progress of seven types of NMAs, and discussed the current challenges of NMAs.

17.
Cell Biol Toxicol ; 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-34458953

RESUMO

OBJECTIVE: The non-coding RNAs have been identified as potential biomarkers in cancers. This study aimed to investigate the effect of long intergenic non-protein coding RNA 467 (LINC00467)/microRNA (miR-125a-3p)/sirtuin 6 (SIRT6) axis on non-small cell lung cancer (NSCLC). METHODS: Expression of LINC00467, miR-125a-3p, SIRT6, and ERK1/2 signaling pathway-related factor in NSCLC was assessed. A549 and H1299 cells were induced by cisplatin (DDP) to establish DDP-resistant cell lines, and the drug resistance was detected. DDP-resistant cells were transfected with relative oligonucleotides and/or plasmids to observe the effects of LINC00467 and miR-125a-3p on NSCLC cells. The interaction among LINC00467, miR-125a-3p, and SIRT6 was detected using dual luciferase reporter gene assay and RNA pull-down assay. A tumor xenograft model was established, and Ki67 expression was detected in tumors. RESULTS: LINC00467 and SIRT6 were upregulated, miR-125a-3p was suppressed, and the ERK1/2 pathway was activated in NSCLC. LINC00467 or SIRT6 inhibition or miR-125a-3p elevation restricted malignancy and DDP resistance of NSCLC cells. Reduced miR-125a-3p reversed the effect of inhibited LINC00467 on NSCLC cells. LINC00467 competitively bound to miR-125a-3p, and miR-125a-3p targeted SIRT6. Inhibiting LINC00467 and SIRT6 or upregulating miR-125a-3p repressed tumor formation in vivo. CONCLUSION: LINC00467 silencing upregulates miR-125a-3p to decrease DDP resistance in NSCLC cells via inhibiting SIRT6 and inactivating the ERK1/2 signaling pathway.

18.
BMC Plant Biol ; 21(1): 374, 2021 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-34388987

RESUMO

BACKGROUND: Cadmium (Cd) is a toxic heavy metal that is harmful to the environment and human health. Cd pollution threatens the cultivation of rice (Oryza sativa L.) in many countries. Improving rice performance under Cd stress could potentially improve rice productivity. RESULTS: In this study, 9 growth traits of 188 different cultivated rice accessions under normal and Cd stress conditions were found to be highly variable during the seedling stage. Based on ~3.3 million single nucleotide polymorphisms (SNPs), 119 Cd-mediated growth response (CGR) quantitative trait loci (QTL) were identified by a genome-wide association study (GWAS), 55 of which have been validated by previously reported QTL and 64 were new CGR loci. Combined with the data from the GWAS, transcriptome analysis, gene annotations from the gene ontology (GO) Slim database, and annotations and functions of homologous genes, 148 CGR candidate genes were obtained. Additionally, several reported genes have been found to play certain roles in CGRs. Seven Cd-related cloned genes were found among the CGR genes. Natural elite haplotypes/alleles in these genes that increased Cd tolerance were identified by a haplotype analysis of a diverse mini core collection. More importantly, this study was the first to uncover the natural variations of 5 GST genes that play important roles in CGRs. CONCLUSION: The exploration of Cd-resistant rice germplasm resources and the identification of elite natural variations related to Cd-resistance will help improve the tolerance of current major rice varieties to Cd, as well as provide raw materials and new genes for breeding Cd-resistant varieties.


Assuntos
Cádmio/farmacologia , Genes de Plantas , Oryza/crescimento & desenvolvimento , Oryza/genética , Poluentes do Solo/farmacologia , Alelos , Perfilação da Expressão Gênica , Genoma de Planta , Estudo de Associação Genômica Ampla , Oryza/efeitos dos fármacos , Desenvolvimento Vegetal/genética , Polimorfismo de Nucleotídeo Único
19.
J Med Chem ; 64(16): 12089-12108, 2021 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-34404206

RESUMO

Poly (ADP-ribose) polymerase-1 (PARP-1) is a potential target for the discovery of chemosensitizers and anticancer drugs. Amentoflavone (AMF) is reported to be a selective PARP-1 inhibitor. Here, structural modifications and trimming of AMF have led to a series of AMF derivatives (9a-h) and apigenin-piperazine/piperidine hybrids (14a-p, 15a-p, 17a-h, and 19a-f), respectively. Among these compounds, 15l exhibited a potent PARP-1 inhibitory effect (IC50 = 14.7 nM) and possessed high selectivity to PARP-1 over PARP-2 (61.2-fold). Molecular dynamics simulation and the cellular thermal shift assay revealed that 15l directly bound to the PARP-1 structure. In in vitro and in vivo studies, 15l showed a potent chemotherapy sensitizing effect against A549 cells and a selective cytotoxic effect toward SK-OV-3 cells through PARP-1 inhibition. 15l·2HCl also displayed good ADME characteristics, pharmacokinetic parameters, and a desirable safety margin. These findings demonstrated that 15l·2HCl may serve as a lead compound for chemosensitizers and the (BRCA-1)-deficient cancer therapy.

20.
Neuroradiology ; 2021 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-34374821

RESUMO

PURPOSE: To explore the functional connectivity (FC) between the bilateral thalamus and the other brain regions in patients with vestibular migraine (VM). METHODS: Resting-state fMRI and 3D-T1 data were collected from 37 patients with VM during the interictal period and 44 age-, gender-, and years of education-matched healthy controls (HC). The FC of the bilateral thalamus was analyzed using a standard seed-based whole-brain correlation method. Furthermore, the correlations between thalamus FC and clinical characteristics of patients were investigated using Pearson's partial correlation. RESULTS: Compared with HC, VM patients showed decreased FC between the left thalamus and the left anterior cingulate cortex (ACC), bilateral insular and right supplementary motor cortex. We also observed decreased FC between the right thalamus and the left insular and ACC in VM patients. Furthermore, patients with VM also exhibited increased FC between the left thalamus and the right precuneus and middle frontal gyrus, between the right thalamus and superior parietal lobule. FC between the right thalamus and the left insular was negatively correlated with disease duration (p = 0.019, r = - 0.399), FC between the left thalamus and the left ACC was negatively correlated with HIT-6 score (p = 0.004, r = - 0.484). CONCLUSION: VM patients showed altered FC between thalamus and brain regions involved in pain, vestibular and visual processing, which are associated with specific clinical features. Specifically, VM patients showed reduced thalamo-pain and thallamo-vestibular pathways, while exhibited enhanced thalamo-visual pathway, which provided first insight into the underlying functional brain connectivity in VM patients.

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