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1.
Medicine (Baltimore) ; 98(52): e18353, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31876709

RESUMO

BACKGROUND: To determine the effectiveness of text message reminders (TMR) on medication adherence (MA) and to investigate the effects of TMR on clinical outcomes. METHODS: The PubMed, Cochrane library, EMbase, and China Biology Medicine databases were searched for randomized-controlled trials with TMR as the intervention for patients with coronary heart disease. Two reviewers independently extracted data and assessed the risk of bias. Meta-analysis was conducted using Stata 15.0 software. RESULTS: In total, 1678 patients in 6 trials were included. Compared with the control group, the MA was 2.85 times greater among the intervention group (RR [relative risk] 2.85; 95% confidence interval [CI] 1.07-7.58). TMR reduced systolic blood pressure (BP) (weighted mean difference) = -6.51; 95% CI -9.79 to -3.23), cholesterol (standard mean difference = -0.26; 95% CI -0.4 to -0.12) and increased the number of patients with BP <140/90 mm Hg (RR 1.39; 95% CI 1.26-1.54). CONCLUSION: TMR significantly promoted MA and reduced systolic BP, cholesterol level, and body mass index, but had no effect on mortality, diastolic BP, or lipoproteins. However, substantial heterogeneity existed in our analyses.


Assuntos
Doença das Coronárias/tratamento farmacológico , Adesão à Medicação , Sistemas de Alerta , Mensagem de Texto , Humanos
2.
Int J Infect Dis ; 87: 154-165, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31442627

RESUMO

OBJECTIVES: It remains uncertain which catheter lock solution (CLS) to prevent catheter-related bloodstream infections (CRBSI) works best and is safest for patients. This study was performed to compare the efficacy of different CLSs for the prevention of CRBSI and ranked these CLSs for practical consideration. METHODS: The PubMed, Web of Science, Embase, and MEDLINE databases, earlier relevant meta-analyses, and the reference lists of included studies were searched. The primary outcome was CRBSI; secondary outcomes were catheter-related thrombosis and exit-site infections. A network meta-analysis was performed to estimate odds ratios (ORs) with 95% confidence intervals (CIs). RESULTS: A total of 52 randomized controlled trials involving 9099 patients and evaluating 13 CLSs (single and combinations) were included. With regard to the quality of the evidence, the risk of bias was typically low or unclear (45 out of 52 trials, 86.5%). In the network meta-analysis, saline (OR 8.44, 95% CI 2.19-32.46), gentamicin+citrate (OR 2.92, 95% CI 1.32-6.42), ethanol (OR 5.33, 95% CI 1.22-23.32), and cloxacillin+heparin (OR 2.07, 95% CI 1.19-5.49) were associated with a greater effect on CRBSI than heparin. CONCLUSIONS: This network meta-analysis showed that minocycline-ethylenediaminetetraacetic acid (EDTA) seemed to be the most effective for the prevention of CRBSI and exit-site infection, and cefotaxime+heparin seemed to be the most effective for catheter-related thrombosis.


Assuntos
Antibacterianos/administração & dosagem , Bacteriemia/prevenção & controle , Infecções Relacionadas a Cateter/prevenção & controle , Humanos , Meta-Análise em Rede , Ensaios Clínicos Controlados Aleatórios como Assunto
3.
Comput Assist Surg (Abingdon) ; 24(sup1): 62-71, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-30661421

RESUMO

Simple fractal dimensions have been proposed for use in the analysis of the characteristics of digitized tongue pictures and tongue coating texture, which could further the establishment of objectified classification criteria under the conditions of expanding sample size. However, detailed descriptions on simple fractal dimensions have been limited. Therefore, BP (back propagation) neural network model classifiers could be designed by further calculation of the multiple fractal spectrum characteristics of digitized tongue pictures in order to classify and recognize the thin/thick or greasy characteristics of tongue coating. The fractal dimensions of sample data of 587 digitized tongue pictures were collected in a standard environment. A statistical analysis was conducted on the calculation results of the sample data, and the sensitivity of the fractal dimensions to the thin/thick and greasy characteristics of digitized tongue pictures was observed. As the overlap region resulted from a range of values of a single parameter, another 8 characteristic parameters of the multiple fractal spectra of the digitized tongue pictures were further proposed as the elements in the input layer of the three-layers BP neural network. Automatic recognition classifiers were designed and trained for the characteristics of digitized tongue pictures and tongue coating textures. The simple fractal dimension was sensitive to the thin/thick and greasy characteristics of digitized tongue pictures and could better judge the characteristics of the thickness of the tongue coating. A classifier with characteristic parameters of multiple fractal spectra as the input vectors identified by the BP neural network models could effectively increase the accuracy rate judged by the characteristics of the tongue coating texture.

4.
Comput Assist Surg (Abingdon) ; : 1-11, 2019 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-30676098

RESUMO

Simple fractal dimensions have been proposed for use in the analysis of the characteristics of digitized tongue pictures and tongue coating texture, which could further the establishment of objectified classification criteria under the conditions of expanding sample size. However, detailed descriptions on simple fractal dimensions have been limited. Therefore, BP (back propagation) neural network model classifiers could be designed by further calculation of the multiple fractal spectrum characteristics of digitized tongue pictures in order to classify and recognize the thin/thick or greasy characteristics of tongue coating.The fractal dimensions of sample data of 587 digitized tongue pictures were collected in a standard environment. A statistical analysis was conducted on the calculation results of the sample data, and the sensitivity of the fractal dimensions to the thin/thick and greasy characteristics of digitized tongue pictures was observed. As the overlap region resulted from a range of values of a single parameter, another eight characteristic parameters of the multiple fractal spectra of the digitized tongue pictures were further proposed as the elements in the input layer of the three-layers BP neural network. Automatic recognition classifiers were designed and trained for the characteristics of digitized tongue pictures and tongue coating textures.The simple fractal dimension was sensitive to the thin/thick and greasy characteristics of digitized tongue pictures and could better judge the characteristics of the thickness of the tongue coating. A classifier with characteristic parameters of multiple fractal spectra as the input vectors identified by the BP neural network models could effectively increase the accuracy rate judged by the characteristics of the tongue coating texture.

5.
Insect Biochem Mol Biol ; 105: 33-42, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30602123

RESUMO

Endoparasitoid wasps deliver a variety of maternal factors, such as venom proteins, viruses, and virus-like particles, from their venom and calyx fluid into hosts and thereby regulate the hosts' immune response, metabolism and development. The endoparasitoid, Microplitis mediator, is used as an important biological agent for controlling the devastating pest Helicoverpa armigera. In this study, using an integrated transcriptomic and proteomic analysis approach, we identified 75 putative venom proteins in M. mediator. The identified venom components were consistent with other known parasitoid wasps' venom proteins, including metalloproteases, serine protease inhibitors, and glycoside hydrolase family 18 enzymes. The metalloprotease and serpin family showed extensive gene duplications in venom apparatus. Isobaric tags for relative and absolute quantitation (iTRAQ) based quantitative proteomics revealed 521 proteins that were differentially expressed at 6 h and 24 h post-parasitism, including 10 wasp venom proteins that were released into the host hemolymph. Further analysis indicated that 511 differentially expressed proteins (DEP) from the host are primarily involved in the immune response, material metabolism, and extracellular matrix receptor interaction. Taken together, our results on parasitoid wasp venoms have the potential to enhance the application of endoparasitoid wasps for controlling insect pest.


Assuntos
Interações Hospedeiro-Parasita , Proteínas de Insetos/metabolismo , Mariposas/parasitologia , Venenos de Vespas/metabolismo , Vespas/metabolismo , Animais , Feminino
6.
Front Immunol ; 9: 2301, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30405599

RESUMO

Parasitoid wasps depend on a variety of maternal virulence factors to ensure successful parasitism. Encapsulation response carried out by host hemocytes is one of the major host immune responses toward limiting endoparasitoid wasp offspring production. We found that VRF1, a metalloprotease homolog venom protein identified from the endoparasitoid wasp, Microplitis mediator, could modulate egg encapsulation in its host, the cotton bollworm, Helicoverpa armigera. Here, we show that the VRF1 proenzyme is cleaved after parasitism, and that the C-terminal fragment containing the catalytic domain enters host hemocytes 6 h post-parasitism. Furthermore, using yeast two-hybrid and pull-down assays, VRF1 is shown to interact with the H. armigera NF-κB factor, Dorsal. We also show that overexpressed of VRF1 in an H. armigera cell line cleaved Dorsal in vivo. Taken together, our results have revealed a novel mechanism by which a component of endoparasitoid wasp venom interferes with the Toll signaling pathway in the host hemocytes.


Assuntos
Hemócitos/metabolismo , Interações Hospedeiro-Parasita , Metaloproteases/metabolismo , Transdução de Sinais , Receptores Toll-Like/metabolismo , Venenos de Vespas/metabolismo , Vespas/metabolismo , Animais , Hemócitos/imunologia , Interações Hospedeiro-Parasita/imunologia , Metaloproteases/imunologia , Modelos Biológicos , Proteólise , Especificidade por Substrato , Venenos de Vespas/química , Venenos de Vespas/imunologia
8.
Insect Sci ; 24(1): 2-16, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26549814

RESUMO

Peptidoglycan recognition proteins (PGRPs) are a family of innate immune receptors that specifically recognize peptidoglycans (PGNs) on the surface of a number of pathogens. Here, we have identified and characterized six PGRPs from endoparasitoid wasp, Microplitis mediator (MmePGRPs). To understand the roles of PGRPs in parasitoid wasps, we analyzed their evolutionary relationship and orthology, expression profiles during different developmental stages, and transcriptional expression following infection with Gram-positive and -negative bacteria and a fungus. MmePGRP-S1 was significantly induced in response to pathogenic infection. This prompted us to evaluate the effects of RNA interference mediated gene specific knockdown of MmePGRP-S1. The knockdown of MmePGRP-S1 (iMmePGRP-S1) dramatically affected wasps' survival following challenge by Micrococcus luteus, indicating the involvement of this particular PGRP in immune responses against Gram-positive bacteria. This action is likely to be mediated by the Toll pathway, but the mechanism remains to be determined. MmePGRP-S1 does not play a significant role in anti-fungal immunity as indicated by the survival rate of iMmePGRP-S1 wasps. This study provides a comprehensive characterization of PGRPs in the economically important hymenopteran species M. mediator.


Assuntos
Proteínas de Transporte/metabolismo , Vespas/imunologia , Animais , Beauveria/imunologia , Proteínas de Transporte/imunologia , Enterobacter cloacae/imunologia , Regulação da Expressão Gênica no Desenvolvimento , Imunidade Inata , Proteínas de Insetos , Micrococcus luteus/imunologia , Filogenia , Interferência de RNA , Vespas/microbiologia
9.
Int J Clin Exp Pathol ; 8(1): 765-71, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25755772

RESUMO

BACKGROUND: Lung cancer is becoming the leading cause of cancer-related deaths with high mortality worldwide and in China as well. Non-small-cell lung cancer (NSCLC) is the most common type of lung cancer accounting for approximately 85% of all cases. Over 70% of cases are at loco-regionally advanced stages or have distant metastasis at the time of presentation with subsequently poor prognosis. MiRNAs are stable molecules in blood and used as biomarkers for the early diagnosis of various malignancy. The purpose of this study was to evaluate whether circulating miR-125a-5p, miR-145 and miR-146a could be used as biomarkers for the diagnosis of NSCLC through measuring their expression and assess their relationship with clinical pathological factors. METHODS: Expression levels of serum miR-125a-5p, miR-145 and miR-146a were detected in 70 pairs of NSCLC patients and healthy controls using quantitative real-time PCR analysis. RESULTS: Serum miR-125a-5p, miR-145 and miR-146a were overexpressed in NSCLC patients compared with healthy controls. Their values of the area under the receiver -operating characteristic curve (AUC-ROC) were 0.71, 0.84 and 0.78. Optimal sensitivity and specificity were 73.53% and 55.71%, 92.75% and 61.43%, 84.06% and 58.57%, respectively in differentiating NSCLC patients from healthy controls. CONCLUSIONS: These preliminary data suggest that serum miR-125a-5p, miR-145 and miR-146a may be useful noninvasive biomarkers for the clinical diagnosis of NSCLC.


Assuntos
Biomarcadores Tumorais/genética , Carcinoma Pulmonar de Células não Pequenas/diagnóstico , Neoplasias Pulmonares/diagnóstico , MicroRNAs/sangue , Adulto , Idoso , Área Sob a Curva , Carcinoma Pulmonar de Células não Pequenas/sangue , Carcinoma Pulmonar de Células não Pequenas/genética , Feminino , Humanos , Neoplasias Pulmonares/sangue , Neoplasias Pulmonares/genética , Masculino , Pessoa de Meia-Idade , Curva ROC , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sensibilidade e Especificidade
10.
Zhongguo Dang Dai Er Ke Za Zhi ; 16(5): 529-33, 2014 May.
Artigo em Chinês | MEDLINE | ID: mdl-24857006

RESUMO

OBJECTIVE: To study the effects of recombinant human interleukin-11 (rhIL-11) on the proliferation and apoptosis of rat intestinal epithelial cell line (IEC-6). METHODS: IEC-6 cells were treated with LPS to establish necrotizing enterocolitis (NEC) model in vitro. rhIL-11 (100 ng/mL) was administered following LPS treatment and these cells were used as the IL-11 treatment group. The cells treated with normal saline only served as the control group. MTT assay was used to determine an optimal concentration (5-200 µg/mL) and time (1-24 h). MTT assay was used to measure the proliferation of IEC-6 cells at 3, 6, 9 and 12 hours after rhIL-11 treatment. Flow cytometry was used to evaluate the apoptosis of IEC-6 cells. RESULTS: IEC-6 cells treated with various concentrations of LPS at various time points showed a lower proliferation than the control group (P<0.05). After 9 hours of rhIL-11 treatment, the proliferation activity of IEC-6 cells in the IL-11 treatment group significantly increased compared with the NEC model group without rhIL-11 treatment (P<0.05), reaching to the level of the control group. The total apoptotic and necrotic rate of IEC-6 cells in the IL-11 treatment group decreased significantly compared with the NEC model group without rhIL-11 treatment (P<0.01), but were still higher than the control group (P<0.05). CONCLUSIONS: rhIL-11 can promote proliferation and reduce apoptotic and necrotic rates of IEC-6 cells treated with LPS.


Assuntos
Interleucina-11/farmacologia , Mucosa Intestinal/efeitos dos fármacos , Lipopolissacarídeos/farmacologia , Animais , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Relação Dose-Resposta a Droga , Mucosa Intestinal/patologia , Necrose , Ratos , Proteínas Recombinantes/farmacologia
11.
Zhongguo Dang Dai Er Ke Za Zhi ; 16(4): 414-7, 2014 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-24750842

RESUMO

OBJECTIVE: To study the timing of presentation and perinatal high-risk factors for necrotizing enterocolitis (NEC) in preterm infants with a gestational age of <33 weeks. METHODS: A case-control study was conducted in 49 preterm infants with NEC (gestational age <33 weeks) who were admitted to the Neonatal Intensive Care Unit of Beijing Bayi Children's Hospital between October 1, 2010 and December 30, 2012, as well as preterm infants without NEC during the same period. The timing of presentation of NEC was retrospectively analyzed, and the perinatal high-risk factors for NEC were determined by multivariate logistic regression analysis. RESULTS: The median age of onset was 17.5 days (range: 3-106 d) in preterm infants with NEC. Sex, being small for gestational age, delivery mode and antenatal corticosteroid therapy were not associated with the development of NEC; low gestational age, low birth weight and neonatal asphyxia increased the risk of NEC, and low gestational age was identified as an independent high-risk factor for the development of NEC. CONCLUSIONS: Low gestational age is an important risk factor for the development NEC in preterm infants under 33 weeks' gestation, and the median age of onset is 17.5 days.


Assuntos
Enterocolite Necrosante/etiologia , Estudos de Casos e Controles , Feminino , Idade Gestacional , Humanos , Recém-Nascido , Recém-Nascido Prematuro , Unidades de Terapia Intensiva Neonatal , Masculino , Fatores de Risco
12.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 20(2): 376-80, 2012 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-22541102

RESUMO

This study was aimed to investigate the pro coagulation effects of hemocoagulase atrix and its effective components (batroxobin and factor X activator) on plasma of normal subjects and patients with bleeding disorders and their mechanisms. Activated partial thromboplastin time (APTT) and prothrombin time (PT) were measured. The factor (F)X activation and thrombin generation were analyzed by using chromogenic substrate method. The results showed that the plasma APTT of normal subjects was shortened by hemocoagulase atrix, batroxobin and FX activator, and the effect of FX activator was found to be concentration-dependent (r = 0.889, P < 0.05). The prolonged APTT of plasma from patients with bleeding disorders could be corrected by hemocoagulase atrix, batroxobin and FX activator, but PT showed no great changes resulted from the treatments. FX activator could promote FX activation and thrombin generation, while neither hemocoagulase atrix nor batroxobin showed such abilities. It is concluded that hemocoagulase atrix promotes coagulation process, and corrects coagulation abnormalities in patients with bleeding disorders, its main component batroxobin directly acts on fibrinogen, and FX activator promotes thrombin generation through activating FX.


Assuntos
Batroxobina/farmacologia , Transtornos da Coagulação Sanguínea/sangue , Coagulação Sanguínea/efeitos dos fármacos , Adulto , Estudos de Casos e Controles , Cisteína Endopeptidases/farmacologia , Fator X/metabolismo , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Proteínas de Neoplasias/farmacologia , Tempo de Tromboplastina Parcial , Trombina/metabolismo , Adulto Jovem
13.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 28(3): 288-90, 2012 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-22394639

RESUMO

AIM: To explore the expression of phosphorylated STAT3 (pSTAT3) in non-small cell lung cancer(NSCLC) and its clinical significance. METHODS: The expression of pSTAT3 was detected in 59 cases of NSCLC and their para-cancerous tissue by immunohistochemical staining. RESULTS: (1) The expression of pSTAT3 in NSCLC was significantly higher than that in para-cancerous tissue. (2) The expression of pSTAT3 was associated with tumor's size and smoking status, which was significantly higher in smaller tumors(diameter<3 cm) and nonsmokers than that in larger tumors(diameter≥3 cm) and smokers, but was not associated with gender, age, lymph node metastasis, clinical stage and differentiation grade. The percentage rate of pSTAT3 expression was higher in adenocarcinoma than that in squamous cell carcinoma but without statistically significant. CONCLUSION: pSTAT3 may play an important role in the development of NSCLC and the direction of targeted therapy.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/metabolismo , Regulação Neoplásica da Expressão Gênica , Neoplasias Pulmonares/metabolismo , Fosfoproteínas/metabolismo , Fator de Transcrição STAT3/metabolismo , Adulto , Idoso , Carcinoma Pulmonar de Células não Pequenas/patologia , Feminino , Humanos , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Carga Tumoral
15.
Ying Yong Sheng Tai Xue Bao ; 21(11): 2836-42, 2010 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-21361007

RESUMO

This paper studied the effects of foliar spraying different concentration La(NO3)3 on the photosynthesis, chlorophyll fluorescence parameters, Mehler reaction, and xanthophyll cycle of ryegrass seedlings under the stress of 150 mmol NaHCO3 x L(-1). Foliar spraying low concentration (0.05 mmol x L(-1)) La (NO3)3 could significantly decrease the decrement of net photosynthesis rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), and stomatal limited value (Ls) and the increment of intercellular CO2 concentration (Ci) under NaHCO3 stress, efficiently alleviate the inhibitory effects of NaHCO3 stress on PS II photochemical quenching (q(p)), actual photochemical efficiency (phi(PS II)), photosynthetic carbon assimilation- dependent electron transport rate (ETRp), and Mehler reaction- dependent electron transport rate (ETRm), enhance the activities of superoxide dismutase, peroxidase, and ascorbate peroxidase, the non-photochemical energy dissipation (NPQ), the xanthophyll cycle pool (V+A+Z), and the de-epoxidation extent of xanthophyll cycle (A+Z)/(V+A+Z), and thereby, alleviate the damage of photosynthetic apparatus caused by NaHCO3 stress. However, treating with high concentration (0.5 mmol x L(-1)) La(NO3)3 had no obvious alleviation effects. It was suggested that foliar spraying an appropriate concentration La (NO3)3 could not only alleviate the decrease of ryegrass seedling' s photosynthetic rate induced by nonstomata factors and the inhibition of photochemical efficiency, but also accelerate the Mehler reaction under NaHCO3 stress. With the accelerated Mehler reaction, excessive excitation energy could directly be consumed, and the xanthophyll cycle-dependent thermal dissipation could be promoted to efficiently protect the photosynthetic apparatus against photo-damage under NaHCO3 stress. Also, the active oxygen produced by the accelerated Mehler reaction could be scavenged by the enhanced anti-oxidative enzyme activities.


Assuntos
Lantânio/farmacologia , Lolium/crescimento & desenvolvimento , Complexo de Proteína do Fotossistema II/metabolismo , Bicarbonato de Sódio/toxicidade , Transporte de Elétrons , Lolium/fisiologia , Fotossíntese/fisiologia , Substâncias Protetoras/farmacologia , Plântula/crescimento & desenvolvimento , Plântula/fisiologia , Estresse Fisiológico
16.
Zhonghua Jie He He Hu Xi Za Zhi ; 32(5): 373-6, 2009 May.
Artigo em Chinês | MEDLINE | ID: mdl-19799074

RESUMO

OBJECTIVE: To evaluate the effect and safety of experimental bronchoscopic lung volume reduction (BLVR) using an one-way valve designed independently. METHODS: The animal study was carried out from June to December 2008. Totally 36 valves were implanted in the target bronchi of 12 healthy male goats. CT scan, artery blood gas exchange and lung pathology were performed after 2, 4, 8 and 12 weeks. RESULTS: All the valves were implanted successfully, but 3 valves were expectorated at the end. The procedure was well tolerated. CT scan showed that for more than 45% (15/33) of the valves, the target lung tissue had collapsed. Lung pathology showed that the alveolar space was deflated and collapsed. Lymphocytes and monocytes infiltrated the interstitial tissue with some degree of fibrosis. CONCLUSIONS: This animal study showed that the independently manufactured one way valve was effective and safe. The one way valve may be useful in the treatment of severe emphysema in the future.


Assuntos
Pneumonectomia/métodos , Doença Pulmonar Obstrutiva Crônica/cirurgia , Enfisema Pulmonar/cirurgia , Animais , Broncoscopia , Cabras , Masculino
17.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 25(1): 27-30, 2009 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-19126384

RESUMO

AIM: To explore the effect of IL-11 on the activation of Jak/STAT pathway and the expressions of Bax and Bcl-2 in the intestinal epithelial cells exposed to neutron radiation. METHODS: The BALB/c mice and IEC-6, irradiated by 4 Gy neutron with or without IL-11 treatment, served as in vivo and in vitro model seperately. The changes of the intestines, activity of Jak1 and STAT3 and expressions of Bax and Bcl-2 were observed by HE staining, Western blot, EMSA, immunohistochemistry and image analysis. RESULTS: (1)Mice exposed to neutron radiation showed severe intestinal damages and no obvious regeneration was seen. IL-11-treated mice had a larger number of cryptal epithelial cells and crypts. (2)Neutron radiation decreased the activities of Jak1 and STAT3, while IL-11 increased their activities. (3) Neutron radiation decreased the expression of Bax and didn't change the level of Bcl-2 in the murine intestine. IL-11 administration decreased the expression of Bax and increased that of Bcl-2. CONCLUSION: The mechanism of the intestinal protection of IL-11 in neutron irradiation might be that IL-11 stimulation triggered activation of Jak/STAT pathway, downregulated the expression of Bax and upregulated the expression of Bcl-2.


Assuntos
Células Epiteliais/efeitos dos fármacos , Células Epiteliais/metabolismo , Interleucina-11/farmacologia , Mucosa Intestinal/citologia , Janus Quinase 1/metabolismo , Nêutrons/efeitos adversos , Fator de Transcrição STAT3/metabolismo , Animais , Western Blotting , Linhagem Celular , Ensaio de Desvio de Mobilidade Eletroforética , Células Epiteliais/efeitos da radiação , Imuno-Histoquímica , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Ratos , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/efeitos da radiação
18.
Ying Yong Sheng Tai Xue Bao ; 19(10): 2103-8, 2008 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-19123341

RESUMO

Taking the secondary natural Pinus tabulaeformis forest in hilly loess region as research object, the shape, size structure, distribution, gap-maker features, and renewal dynamics of gaps were investigated. The results showed that the areas of canopy gap (CG) and extended gap (EG) appeared to have a skewed and small gap- dominated distribution. The CGs had an average area of 31.15 m2, and those with an area of 20-40 m2 made up the highest proportions in number and area, accounting for 38.24% and 30.50%, respectively; while the EGs had an average area of 58.04 m2, and those with an area of 30-60 m2 made up the highest proportions in number and area, accounting for 36.77% and 27.79%, respectively. The average CG area accounted for 53.67% of the average EG area. The gaps were mainly elliptical, and their ages were mainly within 10-20 years, which occupied 33.82% of the total. The gaps were mainly with a height of 14-16 m, and those with a height of 18-22 m made up 36.8% of the total. The gaps were mainly formed by the trees being broken at their bases and the standing dead trees, which made up 47. 66% and 23.44% of all gap-makers, respectively, and thinning and unlawful felling were the major factors in gap formation. The tree deaths from lowered resistance due to tree- ageing, drought, and pest and diseases were one of the reasons for gap formation. The gap-makers per gap numbered 1.89 on average, and most of the gaps were formed by two gap-makers. The gap-makers were mainly Pinus tabulaeformis, followed by Populus davidiana, Betula platyphylla, and Quercus liaotungensis. The diameters of the gap-makers appeared to have a remarkably skewed normal distribution, and the diameters commonly ranged in 10-20 cm and 21-30 cm, taking up 25.0% and 45.31%, respectively. This skewed normal distribution agreed with the skewed area distribution of the gaps. In forest gap, trees had a better regeneration condition, and Chinese pine seedings had no age-discontinuity; while in the understory of Chinese pine, seedings had an obvious discontinuity in age-structure.


Assuntos
Conservação dos Recursos Naturais , Ecossistema , Pinus/crescimento & desenvolvimento , China , Plântula/crescimento & desenvolvimento
19.
World J Gastroenterol ; 12(19): 3055-9, 2006 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-16718787

RESUMO

AIM: To explore the effect of recombinant human interleukin-11 (rhIL-11) on the expressions of interleukin-11 receptor alpha-chain (IL-11Ralpha) and an additional signal transducer glycoprotein 130 (gp130) in intestinal epithelium cell line-6 (IEC-6) after neutron irradiation. METHODS: Cultured IEC-6 cells were exposed to 4.0Gy neutron and treated with 100 ng/mL rhIL-11 12 h prior to or immediately after irradiation. The apoptosis and necrosis rates and expressions of IL-11Ralpha and gp130 were observed by flow cytometry, immunohistochemistry, Western blot and image analysis. RESULTS: The apoptosis rate of IEC-6 cells was increased by irradiation at 6 h (P < 0.01), IL-11 stimulation resulted in a decreased apoptosis rate in irradiated IEC-6 cells (P < 0.05). In normal control IEC-6 cells, intense immunoreactivity of IL-11Ralpha was located within the cell membrane and cytoplasm. The level of IL-11Ralpha expression significantly decreased at 6 h after irradiation (P < 0.01) and restored at 24 h after irradiation. In IEC-6 cells treated with both radiation and rhIL-11, the level of IL-11Ralpha expression was higher than that of irradiated cells (P < 0.05). When it came to gp130 protein, it was located in the cytoplasm of IEC-6 cells. After irradiation, we found a progressive decrease in the expression of gp130 protein (P < 0.05) in 48 h post-radiation, while in rhIL-11-stimulated cells, it came back to normal level at 24 h after irradiation and decreased at 48 h, but was still higher than that of only irradiated cells (P < 0.05). CONCLUSION: rhIL-11 can protect IEC-6 cells from neutron irradiation. The protective effect of rhIL-11 might be connected with its ability to up-regulate the expressions of specific ligand-binding subunit IL-11Ralpha and signal-transducing subunit gp130.


Assuntos
Glicoproteínas/genética , Interleucina-11/farmacologia , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/efeitos da radiação , Nêutrons/efeitos adversos , Receptores de Interleucina/genética , Animais , Apoptose/efeitos dos fármacos , Apoptose/efeitos da radiação , Western Blotting , Linhagem Celular , Citometria de Fluxo , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos da radiação , Glicoproteínas/análise , Humanos , Imuno-Histoquímica , Interleucina-11/análise , Interleucina-11/genética , Subunidade alfa de Receptor de Interleucina-11 , Mucosa Intestinal/química , Mucosa Intestinal/citologia , Lesões por Radiação/prevenção & controle , Ratos , Receptores de Interleucina/análise , Receptores de Interleucina-11 , Proteínas Recombinantes/farmacologia , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologia , Transdução de Sinais/efeitos da radiação
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