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1.
Cell Death Dis ; 12(10): 910, 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34611143

RESUMO

Gastric cancer remains the third leading cause of cancer-related mortality worldwide. Emerging evidence has shown that circular RNAs (circRNAs) play a critical regulatory role in the occurrence and development of various cancers through sponging miRNAs or acting as RNA-binding protein (RBP) sponges. We found that circUBE2Q2 was significantly upregulated in GC tissues and cell lines. Knockdown of circUBE2Q2 inhibited proliferation, migration, invasion, and glycolysis, and increased autophagy in vitro. In addition, knockdown of circUBE2Q2 inhibited GC tumorigenicity and metastasis potential in vivo. A series of experiments were performed to confirm that circUBE2Q2 regulates GC progression via the circUBE2Q2-miR-370-3p-STAT3 axis and promotes tumor metastasis through exosomal communication. Further in vivo experiments confirmed that, combination treatment of circUBE2Q2 knocking down and STAT3 inhibitor has synergistic effects on the gastric cancer growth inhibition, which provides a possibility to enhance the sensitivity of targeted drugs to gastric cancer through targeting circUBE2Q2. Our findings revealed that circUBE2Q2 may serve as a new proliferation-promoting factor and prognostic marker in gastric cancer.

2.
Eur J Surg Oncol ; 2021 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-34625304

RESUMO

BACKGROUND: Using indocyanine green (ICG) fluorescence imaging and tissue marking dyes (TMDs), perigastric lymphatic mapping and their pathological correlation were examined to see whether ICG staining covers all metastatic lymph nodes (LNs) in advanced gastric cancer (AGC). METHODS: Patients with AGC who underwent open distal or total gastrectomy were enrolled. ICG was serially injected intraoperatively into the subserosa along the greater and lesser curvatures. Stomach specimens were examined under a near-infrared camera. ICG-stained LNs were named, excised, and tattooed with different colored TMDs to retrace the exact location after pathological examinations. RESULTS: A total of 687 LNs and 69 LN stations were examined from 11 patients. The map of the perigastric lymphatic network showing the topography of ICG-stained and ICG-unstained LNs, including metastatic information, was successfully reconstructed. The average number of ICG-stained and ICG-unstained LNs were 23.6 ±â€¯12.3 (37.8%) and 38.8 ±â€¯17.1 (62.2%), respectively. LN metastases were present in 28 LN stations of 8 patients. Of 8 cases with LN metastases, 40% (11.1-75% per case) of metastatic LNs were stained by ICG. Of 28 metastatic LN stations, 21 (75.0%) were covered by ICG, and actual metastatic LNs were stained in 16 LN stations (57.1%). In 4/8 cases (50%), all metastatic LN stations showed ICG signals. CONCLUSIONS: ICG fluorescence imaging and TMD are useful tools for visualizing the perigastric lymphatic network and retracing the exact location of ICG-stained LNs in AGC. However, ICG imaging is still not recommended for selective LN dissection in AGC because of the limited staining of perigastric LNs.

3.
Artigo em Inglês | MEDLINE | ID: mdl-34476710

RESUMO

The question of how organisations choose their environmental strategy remains an unsolved problem in institutional theory. We argue that institutional pressure and the characteristics of the firm affect choices around environmental strategies. An empirical analysis of 597 heavily polluting firms indicates that more firms choose environmental leadership strategies, which means that they actively work with upstream and downstream firms to reduce emissions, when government pressure is the greatest; however, more companies are likely to choose pollution prevention strategies, which implies recycling materials in the production process is implemented, when public pressure is the greatest. Finally, organisations with more redundant resources and strong asset specificity are more prone to choose environmental leadership strategies as policy pressure or public pressure increases. The findings offer a decision-making framework to promote environmental measures related to government policy formulation and public participation. The results can also provide empirical evidence to guide environmental strategic choices for heavily polluting enterprises.

4.
Artigo em Inglês | MEDLINE | ID: mdl-34487863

RESUMO

Baylisascaris schroederi, a roundworm (ascaridoid) parasite specific to the bamboo-feeding giant panda (Ailuropoda melanoleuca), represents a leading cause of mortality in wild populations. Here, we present a 293-Mb chromosome-level genome assembly of B. schroederi to infer its biology, including host adaptations. Comparative genomics revealed an evolutionary trajectory accompanied by host-shift events in ascaridoid parasite lineages after host separations, suggesting their potential for transmission and rapid adaptation to new hosts. Genomic and anatomical lines of evidence, including expansion and positive selection of genes related to the cuticle and basal metabolisms, indicated that B. schroederi undergoes specific adaptations to survive in the sharp-edged bamboo enriched gut of giant pandas by structurally increasing its cuticle thickness and efficiently utilizing host nutrients through gut parasitism. Additionally, we characterized the secretome of B. schroederi and predicted potential drug and vaccine targets for new control strategies. Overall, this genome resource provides new insights into the host adaptation of B. schroederi to the giant panda as well as the host-shift events in ascaridoid parasite lineages. Our findings on B. schroederi's unique biology will also aid in the development of prevention and treatment methods to protect giant panda populations from roundworm parasitism.

5.
Cell Death Differ ; 2021 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-34511601

RESUMO

Gasdermin-D (GSDMD), the executioner of pyroptotic cell death when it is cleaved by inflammatory caspases, plays a crucial role in host defense and the response to danger signals. So far, there are no known mechanisms, other than cleavage, for regulating GSDMD. Here, we show that tripartite motif protein TRIM21 acts as a positive regulator of GSDMD-dependent pyroptosis. TRIM21 interacted with GSDMD via its PRY-SPRY domain, maintaining GSDMD stable expression in resting cells yet inducing the N-terminus of GSDMD (GSDMD-N) aggregation during pyroptosis. TRIM21-deficient cells displayed a reduced cell death in response to NLRP3 or NLRC4 inflammasome activation. Genetic ablation of TRIM21 in mice conferred protection from LPS-induced inflammation and dextran sulfate sodium-induced colitis. Therefore, TRIM21 plays an essential role in GSDMD-mediated pyroptosis and may be a viable target for controlling and treating inflammation-associated diseases.

6.
Clin Biomech (Bristol, Avon) ; 89: 105475, 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-34525448

RESUMO

BACKGROUND: Subtalar arthroereisis refers to the implantation of a sinus tarsi implant for the treatment of flexible flatfoot. The purpose of this study was to compare the ability to correct the flatfoot deformity and contact pressure of the posterior subtalar joint between two types of self-locking wedge implants and between two different positions for the same device in a cadaveric flatfoot model. METHODS: The flatfoot model was created in ten cadaver feet through ligament sectioning and cyclic loading. Three kinds of arthroereisis procedures were evaluated: Talar-Fit (type I self-locking wedge implant) anchored in the sinus portion of the tarsal sinus (T-sinus group), Talar-Fit in the canalis portion (T-canalis group), and HyProCure (type II) in the canalis portion (H group). Corrective ability in the sagittal and transverse planes were measured with clinometers. Contact pressure was measured with pressure-sensitive films. FINDINGS: T-canalis group provided more sagittal (mean difference for size 10 mm: 1.9°, P = 0.014; mean difference for size 11 mm: 3.1°, P = 0.037) and transverse (mean difference for size 8 mm: 1.8°, P = 0.049; mean difference for size 11 mm: 2.2°, P = 0.049) corrections than T-sinus group. The flattening process shifted the peak pressure of the posterior subtalar joint to the posteromedial side (P < 0.05) and arthroereisis helped the distribution of contact pressure restore uniformity (all P > 0.05). INTERPRETATION: A self-locking wedge implant inserted in the canalis portion of the tarsal sinus achieved better correction than an implant inserted in the sinus portion.

7.
Am J Manag Care ; 27(9): e330-e335, 2021 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-34533916

RESUMO

OBJECTIVES: Whether and how the COVID-19 pandemic affected utilization of routine medical care in areas with low infection risk, such as Taiwan, has not been widely addressed. We aimed to evaluate the impact of the COVID-19 pandemic on access to medical care. STUDY DESIGN: Before and after exposure (COVID-19 pandemic) design with a historical control group for comparison of clinical visits based on a retrospective cohort of 6722 customary patients of a community hospital in Zhunan, Taiwan. METHODS: Repeated measurements of medical utilization in 4-month periods (January to April) of 2019 and 2020 in light of the emerging COVID-19 pandemic were collected. Access to medical care was defined as the mean frequencies of clinical visits. The impacts of the COVID-19 pandemic on access in the overall and specific groups were quantified with a multivariable Poisson regression model. RESULTS: The overall outpatient visits per month declined by 39% (rate ratio [RR], 0.61; P < .0001) after adjusting for demographics. A notable reduction in visits was observed in foreign patients (RR, 0.50; P < .0001). The visits of the elderly (≥ 80 years) were the most frequent before the COVID-19 pandemic but were reduced by 44% (RR, 0.56; P < .0001) after it began. Most disease categories revealed a declining trend, but the size of reduction varied by International Classification of Diseases codes. CONCLUSIONS: The COVID-19 pandemic prevented some individuals from keeping regular medical appointments even in an area with a low infection risk. Our findings imply that more research is required to mitigate the effects of delayed medical care for patients who infrequently utilized medical care during and after the long-lasting pandemic period.


Assuntos
COVID-19 , Pandemias , Idoso , Assistência Ambulatorial , Humanos , Estudos Retrospectivos , SARS-CoV-2
8.
Comput Intell Neurosci ; 2021: 8292535, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34567104

RESUMO

In the Panax notoginseng quality intelligent management system, the big roots and fibrous roots cannot be cut automatically because the machine cannot distinguish the taproot, big roots, and fibrous roots of Panax notoginseng, resulting in the automatic cutting mechanism unable to obtain the control trajectory coordinate reference of the tool feed. To solve this problem, this paper proposes a visual optimal network model detection method, which uses the image detection method of marking anchor frames to improve the detection accuracy. A variety of deep learning network models are modified by the TensorFlow framework, and the best training model is optimized by comparing the results of training, testing, and verification data. This model is used to automatically identify the taproots and provide the control trajectory coordinate reference for the actuator that cuts big roots and fibrous roots automatically. The experimental results show that the optimal network model studied in this paper is effective and accurate in identifying the taproots of Panax notoginseng.


Assuntos
Panax notoginseng , Raízes de Plantas , Tecnologia
9.
J Agric Food Chem ; 69(38): 11196-11203, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34528796

RESUMO

Improving the concentration and bioavailability of zinc (Zn) in cereal grains is an important way to solve the problem of Zn deficiency in human body. The bioavailability of Zn is related to both its distribution and speciation in grains. In the current study, we examined the differences of Zn concentration, distribution, and speciation within grains among wheat cultivars with similar high grain yield but contrasting grain Zn concentration using synchrotron micro X-ray fluorescence (µ-XRF) and X-ray absorption near-edge structure (XANES). Results showed that compared to the low-Zn cultivar, the Zn concentration was 103, 50, 76, 33, and 64% higher in the crease region, aleurone layer, scutellum, embryonic axis, and endosperm of the high-Zn cultivar, respectively. Zinc mainly colocalized with phosphorus (P) in the aleurone layer and the scutellum, but less colocalization of Zn with P and a much lower concentration ratio of P/Zn were found in the high-Zn cultivar. Sulfur (S) is present in the form of scattered spots in the endosperm in accord with Zn, but the colocalization of Zn with S was predominant in the modified aleurone layer and the nucellar projection of the high-Zn cultivar. XANES results showed the lower proportion of Zn-phytate in the high-Zn cultivar. Findings indicated that it is possible to select the high-yield wheat cultivar with both high grain Zn concentration and high bioavailability, which provide a new perspective for genetic Zn biofortification.


Assuntos
Grão Comestível , Triticum , Biofortificação , Disponibilidade Biológica , Humanos , Zinco
10.
Acta Biochim Biophys Sin (Shanghai) ; 53(10): 1321-1332, 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34508625

RESUMO

The fusion gene of ABL1 is closely related to tumor proliferation, invasion, and migration. It has been reported recently that ABL1 itself is required for T-cell acute lymphoblastic leukemia (T-ALL) cell migration induced by CXCL12. Further experiments revealed that ABL1 inhibitor Nilotinib inhibited leukemia cell migration induced by CXCL12, indicating the possible application of Nilotinib in T-ALL leukemia treatment. However, the interacting proteins of ABL1 and the specific mechanisms of their involvement in this process need further investigation. In the present study, ABL1 interacting proteins were characterized and their roles in the process of leukemia cell migration induced by CXCL12 were investigated. Co-immunoprecipitation in combination with mass spectrometry analysis identified 333 proteins that interact with ABL1, including Cofilin1. Gene ontology analysis revealed that many of them were enriched in the intracellular organelle or cytoplasm, including nucleic acid binding components, transfectors, or co-transfectors. Kyoto Encyclopedia of Genes and Genomes analysis showed that the top three enriched pathways were translation, glycan biosynthesis, and metabolism, together with human diseases. ABL1 and Cofilin1 were in the same complex. Cofilin1 binds the SH3 domain of ABL1 directly; however, ABL1 is not required for the phosphorylation of Cofilin1. Molecular docking analysis shows that ABL1 interacts with Cofilin1 mainly through hydrogen bonds and ionic interaction between amino acid residues. The mobility of leukemic cells was significantly decreased by Cofilin1 siRNA. These results demonstrate that Cofilin1 is a novel ABL1 binding partner. Furthermore, Cofilin1 participates in the migration of leukemia cells induced by CXCL12. These data indicate that ABL1 and Cofilin1 are possible targets for T-ALL treatment.

11.
J Environ Manage ; 300: 113703, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34509818

RESUMO

Wetland plants play a major role in the process of wastewater treatment in constructed wetlands (CWs). The inhibitory effect of salt stress on plants may reduce the performance of CWs. In this study, salicylic acid (SA) and/or calcium ion (Ca2+) were used for root pretreatment to alleviate the salt stress in Iris pseudacorus L. The results showed that root pretreatment with SA and/or Ca2+ improved the response of Iris pseudacorus L. to salinity by increasing growth, photosynthetic pigments, Pro content, enzymes activities and K+ content. In addition, SA and/or Ca2+ application in saline conditions decreased the relative conductivity and content of malondialdehyde. RNA-seq analysis showed the expression of hormone signaling genes, potassium ion transporter genes, oxidative stress genes and photosynthesis genes were up-regulated after pretreating with SA and CaCl2. In conclusion, the addition of SA and Ca2+ could improve the saline wastewater treatment efficiency of CWs by enhancing the salt tolerance of Iris pseudacorus L.


Assuntos
Iris (Planta) , Áreas Alagadas , Cloreto de Cálcio , Iris (Planta)/genética , Ácido Salicílico/farmacologia , Tolerância ao Sal/genética
12.
Sleep Med ; 85: 184-190, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34343768

RESUMO

STUDY OBJECTIVES: We aim to explore the mechanism of relationship between insomnia and liver metabolism by examining the gene × insomnia interactions. METHODS: Individual level genotypic and phenotypic data were obtained from the UK Biobank cohort. Regression analysis was first conducted to test the association of insomnia with plasma total bilirubin (TBil; n = 186,793), direct bilirubin (DBil; n = 159,854) and total protein (TP; n = 171,574) in UK Biobank cohort. Second, genome-wide gene-environment interaction study (GWGEIS) was conducted by PLINK 2.0, and FUMA platform was used to identify enriched pathway terms. RESULTS: In UK Biobank cohort, we found that TP (P < 2.00 × 10-16), DBil (P = 1.72 × 10-3) and TBil (P = 3.38 × 10-5) were significantly associated with insomnia. GWGEIS of both DBil and TBil observed significant G × INSOMNIA effects between insomnia and UDP Glucuronosyltransferase Family 1 (rs6431558, P = 6.26 × 10-11) gene. GWGEIS of TP also detected several significant genes interacting with insomnia, such as KLF15, (rs70940816, P = 6.77 × 10-10) and DOK7, (rs2344205, P = 1.37 × 10-9). Multiple gene ontology (GO) terms were identified for bilirubin, such as GO_URONIC_ACID_METABOLIC_PROCESS (adjusted P = 4.15 × 10-26). CONCLUSION: Our study results suggested negative associations between insomnia and DBil and TBil; and a positive association between insomnia and TP.

13.
J Agric Food Chem ; 69(35): 10215-10222, 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34415758

RESUMO

High-purity docosahexaenoic acid (DHA) resources are insufficient in the pharmaceutical and food industries. Although many efforts have attempted to obtain the high-purity DHA production, few reports have been successful. Here, a combined metabolic engineering strategy was employed to increase the DHA purity in the oleaginous thraustochytrid Aurantiochytrium. The strategy includes both partial deactivation of the competing pathway of DHA biosynthesis, by disrupting one copy of the fatty acid synthase gene, and strengthening of substrate supply and triacylglycerol synthesis, by the overexpression of acetyl-CoA carboxylase and diacylglycerol acyltransferase. With this strategy, a final mutant was obtained with a DHA purity of 61% in total fatty acids and a content of 331 mg/g dry cell weight. This study provides an advanced strategy for sustainable high-purity DHA production and highlights the strategy for producing designer oils in industrial oleaginous microorganisms.


Assuntos
Ácidos Docosa-Hexaenoicos , Estramenópilas , Ácidos Graxos , Alimentos , Engenharia Metabólica , Estramenópilas/genética
14.
Int J Sports Med ; 2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-34416779

RESUMO

High-volume training followed by inadequate recovery may cause overtraining. This process may undermine the protective effect of regular exercise on the cardiovascular system and may increase the risk of pathological cardiac remodelling. We evaluated whether chronic overtraining changes cardiac-related microRNA profiles in the left and right ventricles. C57BL/6 mice were divided into the control, normal training, and overtrained by running without inclination, uphill running or downhill running groups. After an 8-week treadmill training protocol, the incremental load test and training volume results showed that the model had been successfully established. The qRT-PCR results showed increased cardiac miR-1, miR-133a, miR-133b, miR-206, miR-208b and miR-499 levels in the left ventricle of the downhill running group compared with the left ventricle of the control group. Similarly, compared with the control group, the downhill running induced increased expression of miR-21, miR-17-3p, and miR-29b in the left ventricle. Unlike the changes in the left ventricle, no difference in the expression of the tested miRNAs was observed in the right ventricle. Briefly, our results indicated that overtraining generally affects key miRNAs in the left ventricle (rather than the right ventricle) and that changes in individual miRNAs may cause either adaptive or maladaptive remodelling with overtraining.

15.
Environ Pollut ; 289: 117812, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34333269

RESUMO

In this study, the biochar (BC) supported Fe-Cu bimetallic stabilized by PVP (Fe-Cu/PVP/BC) were prepared and utilized to enhance the nitrate (NO3-) removal and the selectivity toward nitrogen (N2). Results showed the optimum Fe:Cu:BC ratio and the dosage of the BC (pyrolysis at 700 °C) supported Fe-Cu bimetallic stabilized by polyvinylpyrrolidone (PVP) (Fe-Cu/PVP/BC700) were respectively 1:2:3 and 1 mg L-1 with the selectivity toward N2 of 31 %. This was mainly due to the synergy among Fe0, Cu0 and BC in the Fe-Cu/PVP/BC. The addition of Fe0 could reduce the NO3- through providing electron. The Cu0 and BC improved the selectivity of NO3- to N2 through forming [Cu-NO2-ads] and adjusting redox potential. The addition of Fe-Cu/PVP/BC could supply electrons for denitrification and enhance the relative abundances of Azospira and Thauera related to denitrification to improve NO3- removal. This result was further confirmed by the variations of denitrifying functional genes (narG, nirK, nirS and nosZ). This research provided an effective method to improve NO3- removal during surface water treatment in constructed wetlands (CWs) by adding Fe-Cu/PVP/BC.


Assuntos
Nitrogênio , Áreas Alagadas , Carvão Vegetal , Desnitrificação , Nitratos , Povidona , Eliminação de Resíduos Líquidos
16.
J Environ Manage ; 299: 113559, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34438309

RESUMO

Pesticide and heavy metal contaminants, such as chlorpyrifos (CP) and cadmium (Cd) in farmland drainage had caused the water pollution and attracted extensive concerns around the world. The incorporation of zeolite-based iron-carbon (ZB-IC) into constructed wetlands (CWs) was prepared to simultaneously remove chlorpyrifos (CP) and cadmium (Cd) in farmland drainage, and the interaction of CP degradation and Cd removal was investigated. Laboratory simulated experiments were carried out in this study, and the results presented that the removal efficiencies of CP and Cd by ZB-IC coupled CWs (ZB-IC-CW) were 99.55% and 98.59%, respectively, which were much higher than that of the zeolite-based (ZB) CWs (CP = 92.99%; Cd = 63.54%). The removal mechanism of CP and Cd by ZB-IC substrate was mainly attributed to electron transfer, which occurred from iron corrosion and hydrogen generation process. In addition, CP could act as carbon source to promote denitrification process. Microbial analysis revealed that the relative abundances of CP-resistant bacteria (Firmicutes, Clostridia and Acetobacterium), Cd-resistant bacteria (Bacteroidetes) and denitrifying bacteria (Proteobacteria and Patescibacteria) were dramatically increased due to the addition of ZB-IC. The higher czcA gene and opd gene in ZB-IC-CW demonstrated that the addition of CP played a positive role in Cd removal, while Cd showed slightly affect to CP removal.


Assuntos
Clorpirifos , Áreas Alagadas , Cádmio , Carbono , Desnitrificação , Fazendas , Ferro , Nitrogênio , Eliminação de Resíduos Líquidos , Águas Residuárias
17.
Environ Toxicol ; 36(11): 2322-2332, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34418280

RESUMO

Long noncoding RNAs (lncRNAs) are known to be the important regulators in cancer progression. However, the role of lncRNA FAM66C (FAM66C) is yet to be investigated in intrahepatic cholangiocarcinoma (ICC). This study aimed to investigate the effects and related mechanisms of FAM66C in ICC. Human ICC tissues and cell lines were collected. The expression levels of FAM66C, hsa-miR-23b-3p (miR-23b-3p), and KCND2 were detected by qRT-RCR. The transfection experiments were employed to measure the effect of FAM66C on cell viabilities, migration, and invasion in ICC cells by CCK-8, transwell assays. Glycolysis was investigated by glucose consumption, lactate production and ATP levels. The dual-luciferase reporter and RNA pull down assays were conducted as a means of confirming the interactions between FAM66C, miR-23b-3p, and KCND2. Furthermore, the levels of the EMT-associated proteins (KCND2, GLUT1, PKM2, and LDHA) in ICC cells were detected by western blot. FAM66C was increased in ICC tissues and cells, increased cell viability, glycolysis, migration and invasion, and decreased apoptosis were shown in FAM66C overexpressing cells. Mechanistic analyses revealed that FAM66C regulated the downstream target gene KCND2 by sponging miR-23b-3p. FAM66C effect on ICC was further validated in murine xenograft assays. FAM66C knockdown cells gave rise to tumors that were smaller in size, consistent with the role of FAM66C as a promoter of in vivo tumor growth. These data revealed that FAM66C was able to drive ICC tumor progression and glycolytic activity via the miR-23b-3p/KCND2 axis, indicating FAM66C may be a viable target for treating ICC.


Assuntos
Neoplasias dos Ductos Biliares , Colangiocarcinoma , MicroRNAs , RNA Longo não Codificante , Animais , Neoplasias dos Ductos Biliares/genética , Ductos Biliares Intra-Hepáticos/metabolismo , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Colangiocarcinoma/genética , Regulação Neoplásica da Expressão Gênica , Glicólise/genética , Humanos , Camundongos , MicroRNAs/genética , MicroRNAs/metabolismo , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Canais de Potássio Shal
18.
Artigo em Inglês | MEDLINE | ID: mdl-34339842

RESUMO

Wild castor grows in the high-altitude tropical desert of the African Plateau, a region known for high ultraviolet radiation, strong light, and extremely dry condition. To investigate the potential genetic basis of adaptation to both highland and tropical deserts, we generated a chromosome-level genome sequence assembly of the wild castor accession WT05, with a genome size of 316 Mb, a scaffold N50 of 31.93 Mb, and a contig N50 of 8.96 Mb, respectively. Compared with cultivated castor and other Euphorbiaceae species, the wild castor exhibits positive selection and gene family expansion for genes involved in DNA repair, photosynthesis, and abiotic stress responses. Genetic variations associated with positive selection were identified in several key genes, such as LIG1, DDB2, and RECG1, involved in nucleotide excision repair. Moreover, a study of genomic diversity among wild and cultivated accessions revealed genomic regions containing selection signatures associated with the adaptation to extreme environments. The identification of the genes and alleles with selection signatures provides insights into the genetic mechanisms underlying the adaptation of wild castor to the high-altitude tropical desert and would facilitate direct improvement of modern castor varieties.

19.
Artigo em Inglês | MEDLINE | ID: mdl-34347594

RESUMO

This paper presents a novel framework to recover detailed avatar from a single image. It is a challenging task due to factors such as variations in human shapes, body poses, texture and viewpoints. Prior methods typically attempt to recover the human body shape using a parametric based template that lacks the surface details. As such the resulting body shape appears to be without clothing. In this paper, we propose a novel learning-based framework that combines the robustness of parametric model with the flexibility of free-form 3D deformation. We use the deep neural networks to refine the 3D shape in a Hierarchical Mesh Deformation (HMD) framework, utilizing the constraints from body joints, silhouettes, and per-pixel shading information. We are able to restore detailed human body shapes with complete textures beyond skinned models. Experiments demonstrate that our method has outperformed previous state-of-the-art approaches, achieving better accuracy in terms of both 2D IoU number and 3D metric distance.

20.
Nat Commun ; 12(1): 4347, 2021 07 23.
Artigo em Inglês | MEDLINE | ID: mdl-34301933

RESUMO

Heterologous expression of biosynthetic gene clusters (BGCs) avails yield improvements and mining of natural products, but it is limited by lacking of more efficient Gram-negative chassis. The proteobacterium Schlegelella brevitalea DSM 7029 exhibits potential for heterologous BGC expression, but its cells undergo early autolysis, hindering further applications. Herein, we rationally construct DC and DT series genome-reduced S. brevitalea mutants by sequential deletions of endogenous BGCs and the nonessential genomic regions, respectively. The DC5 to DC7 mutants affect growth, while the DT series mutants show improved growth characteristics with alleviated cell autolysis. The yield improvements of six proteobacterial natural products and successful identification of chitinimides from Chitinimonas koreensis via heterologous expression in DT mutants demonstrate their superiority to wild-type DSM 7029 and two commonly used Gram-negative chassis Escherichia coli and Pseudomonas putida. Our study expands the panel of Gram-negative chassis and facilitates the discovery of natural products by heterologous expression.


Assuntos
Produtos Biológicos/metabolismo , Burkholderiales/genética , Genoma Bacteriano/genética , Família Multigênica/genética , Proteobactérias/genética , Burkholderiaceae/genética , Burkholderiaceae/metabolismo , Burkholderiales/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Regulação Bacteriana da Expressão Gênica , Engenharia Genética/métodos , Mutação , Policetídeos/metabolismo , Proteobactérias/metabolismo , Pseudomonas putida/genética , Pseudomonas putida/metabolismo
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