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1.
J Nanosci Nanotechnol ; 20(2): 909-917, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383086

RESUMO

Tailored broussonetia-like NiCo2O4 is grown on carbon cloth using tri-sodium citrate assisted hydrothermal method. The chelating effect of citric ions has been utilized to investigate the morphological and structural evolution of NiCo2O4 on carbon cloth, which have been illustrated by scanning electron microscopy, transmission electron microscopy, and X-ray photoelectron spectra. The results demonstrate that the morphological alteration of NiCo2O4 from single nanowire to broussonetia-like structure has been detected after the addition of tri-sodium citrate. Citric ion plays a crucial role as an electrostatic stabilizer in determining this unique structure. When used as binder-free electrode in aqueous supercapacitors, the broussonetia-like NiCo2O4 electrode exhibits a specific capacitance of 527.9 F g-1 at a current density of 0.5 A g-1. Additionally, an asymmetric supercapacitor is further assembled using NiCo2O4 as the positive electrode and activated carbon as negative electrode. The device exhibits a maximum energy of 26.4 Wh kg-1 at power density of 800 W kg-1. A long-term cycling stability with 82% capacitance retention is maintained after 20,000 cycles at a current density of 5 A g-1, indicating the practical applicability of the tested device.

2.
Org Lett ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31747296

RESUMO

A highly reactive bis-naphthalene tetracarboxylic diimide (bis-NDI) intermediate, TBrDNDI, was designed and synthesized for core-expanded NDIs. Based on this intermediate, we achieved 9- and 11-membered core-expanded bis-NDI derivatives. Through expanding the NDI core and introducing electron-donor or electron-acceptor groups, the frontier energy orbitals, optical and electrical properties of these bis-NDIs can be finely tuned to obtain air-stable ambipolar or n-type materials.

3.
Adv Mater ; 31(44): e1904369, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31538380

RESUMO

Owing to the intense charge repulsion of multivalent ions and intrinsic slugggish kenetics, vast and fast storage of zinc ions into electrode materials has remained unattainable. Here, an efficient strategy to unlock the electrochemical activity of rocksalt vanadium oxynitride is developed via the substitution of low-valent oxygen for high-valent nitrogen, forming disordered rocksalt with abundant vacancies/defects due to the charge-compensating function. Unexpectedly, the disordered rocksalt not only provides plentiful active sites for zinc ions but is also beneficial for the rapid diffusion of zinc ions, owing to the large presence of vacancies/defects in the matrix. Hence, a very high reversible capacity (603 mAh g-1 , 0.2C) and high rate capability (124 mAh g-1 at 600C) are achieved for zinc storage. This should open a new and efficient avenue for the design of electrode materials with both high energy and power densities for aqueous zinc-ion batteries.

4.
J Med Entomol ; 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31504655

RESUMO

Sarcophaga peregrina is an important flesh fly species for estimating the minimum postmortem interval (PMImin) in forensic entomology. The accurate determination of the developmental age is a crucial task for using necrophagous sarcophagids to estimate PMImin. During larval development, the age determination is straight forward by the morphological changes and variation of length, weight, and width; however, the age estimation of sarcophagid intrapuparial is more difficult due to anatomical and morphological changes not being visible. The analysis of differentially expressed genes (DEGs) during sarcophagid metamorphosis is a potential method for age estimation of intrapuparial. In the present study, real-time quantitative polymerase chain reaction (RT-qPCR) was used to analyze the differential gene expression level of S. peregrina intrapuparial in different constant temperatures (35°C, 25°C, and 15°C). In addition, the appropriate reference genes of S. peregrina were selected in the intrapuparial and at different temperatures to obtain reliable and valid gene expression profiles. The results indicated that two candidate genes (18S rRNA and 28S rRNA) were the most reliable reference genes, and four DEGs (Hsp90, A-alpha, AFP, AFBP) have the potential to be used to more accuracy estimate the age of S. peregrina intrapuparial.

5.
Virol Sin ; 34(5): 508-520, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31215001

RESUMO

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a highly pathogenic tick-borne bunyavirus that causes lethal infectious disease and severe fever with thrombocytopenia syndrome (SFTS) in humans. The molecular mechanisms and host cellular factors required for SFTSV infection remain uncharacterized. Using a genome-wide CRISPR-based screening strategy, we identified a host cellular protein, sorting nexin 11 (SNX11) which is involved in the intracellular endosomal trafficking pathway, as an essential cell factor for SFTSV infection. An SNX11-KO HeLa cell line was established, and SFTSV replication was significantly reduced. The glycoproteins of SFTSV were detected and remained in later endosomal compartments but were not detectable in the endoplasmic reticulum (ER) or Golgi apparatus. pH values in the endosomal compartments of the SNX11-KO cells increased compared with the pH of normal HeLa cells, and lysosomal-associated membrane protein 1 (LAMP1) expression was significantly elevated in the SNX11-KO cells. Overall, these results indicated that penetration of SFTSV from the endolysosomes into the cytoplasm of host cells was blocked in the cells lacking SNX11. Our study for the first time provides insight into the important role of the SNX11 as an essential host factor in the intracellular trafficking and penetrating process of SFTSV infection via potential regulation of viral protein sorting, membrane fusion, and other endocytic machinery.

6.
EBioMedicine ; 45: 81-91, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31208947

RESUMO

BACKGROUND: The neddylation pathway is overactivated in human cancers. Inhibition of neddylation pathway has emerged as an attractive anticancer strategy. The mechanisms underlying neddylation overactivation in cancer remain elusive. MLN4924/Pevonedistat, a first-in-class NEDD8-activating enzyme (NAE, E1) inhibitor, exerts significant anti-tumor effects, but its mutagenic resistance remains unresolved. METHODS: The expression of NEDD8-conjugating enzyme UBC12/UBE2M (E2) and NEDD8 were estimated by bioinformatics analysis and western blot in human lung cancer cell lines. The malignant phenotypes of lung cancer cells were evaluated both in vitro and in vivo upon UBC12 knockdown. Cell-cycle arrest was evaluated by quantitative proteomic analysis and propidium iodide stain and fluorescence - activated cell sorting (FACS). The growth of MLN4924 - resistant H1299 cells was also evaluated upon UBC12 knockdown. FINDINGS: The mRNA level of UBC12 in lung cancer tissues was much higher than that in normal lung tissues, increased with disease deterioration, and positively correlated with NEDD8 expression. Moreover, the overexpression of UBC12 significantly enhanced protein neddylation modification whereas the downregulation of UBC12 reduced neddylation modification of target proteins. Functionally, neddylation inactivation by UBC12 knockdown suppressed the malignant phenotypes of lung cancer cells both in vitro and in vivo. The quantitative proteomic analysis and cell cycle profiling showed that UBC12 knockdown disturbed cell cycle progression by triggering G2 phase cell-cycle arrest. Further mechanistical studies revealed that UBC12 knockdown inhibited Cullin neddylation, led to the inactivation of CRL E3 ligases and induced the accumulation of tumor-suppressive CRL substrates (p21, p27 and Wee1) to induce cell cycle arrest and suppress the malignant phenotypes of lung cancer cells. Finally, UBC12 knockdown effectively inhibited the growth of MLN4924-resistant lung cancer cells. INTERPRETATION: These findings highlight a crucial role of UBC12 in fine-tuned regulation of neddylation activation status and validate UBC12 as an attractive alternative anticancer target against neddylation pathway. FUND: Chinese Minister of Science and Technology grant (2016YFA0501800), National Natural Science Foundation of China (Grant Nos. 81401893, 81625018, 81820108022, 81772470, 81572340 and 81602072), Innovation Program of Shanghai Municipal Education Commission (2019-01-07-00-10-E00056), Program of Shanghai Academic/Technology Research Leader (18XD1403800), National Thirteenth Five-Year Science and Technology Major Special Project for New Drug and Development (2017ZX09304001). The funders had no role in study design, data collection, data analysis, interpretation, writing of the report.

7.
BMC Infect Dis ; 19(1): 476, 2019 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-31138131

RESUMO

BACKGROUND: Severe fever with thrombocytopenia syndrome (SFTS) is a newly identified severe infectious disease caused by SFTS phlebovirus (SFTSV). SFTS monitoring has been carried out since 2010 in mainland China. We analysed the detection results of SFTSV RNA and antibody in SFTS surveillance cases to provide basic data for SFTS diagnosis. METHODS: This study was conducted in Shandong Province. Sera of SFTS surveillance cases were collected to detect SFTSV RNA and antibody by real-time RT-PCR and enzyme-linked immunosorbent assay, respectively. Detection rates were calculated. SPSS 18.0 (Chicago, IL, USA) was used for statistical analysis to compare the detection rates of SFTSV RNA and antibodies among different sera groups. RESULTS: A total of 374 SFTS surveillance cases were enrolled. Overall, 93.3% (349/374) of the sera samples were collected within 2 weeks after onset, and 6.7% (25/374) were collected between 15 days and 45 days. Of these, 183 (48.9%) were positive for SFTSV RNA. The SFTSV RNA-positive rate peaked (52.2%) in samples collected ≤7 days after onset and then showed a decreasing trend. The detection rate of SFTSV-specific IgM antibody was 30.5% (46/151) and was highest in samples collected between 8 and 14 days (43.3%, 26/60). The positive rate of SFTSV-specific IgG antibody (17.9%, 27/151) showed an increasing trend with the specimen collection time. In total, 74.8% (113/151) of sera samples had the same SFTSV RNA and IgM antibody detection results. However, 23.2% (29/125) of SFTSV RNA-negative cases were IgM antibody-positive, and 8.6% (9/105) of IgM antibody-negative cases were SFTSV RNA-positive. CONCLUSIONS: SFTSV RNA detection was preferred for SFTSV infection during disease surveillance. For highly suspected SFTS cases, IgM antibody is suggested to make a comprehensive judgement.


Assuntos
Anticorpos Antivirais/sangue , Infecções por Bunyaviridae/epidemiologia , Infecções por Bunyaviridae/virologia , Phlebovirus/genética , RNA Viral/sangue , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , China/epidemiologia , Ensaio de Imunoadsorção Enzimática/métodos , Feminino , Febre/virologia , Humanos , Imunoglobulina M/sangue , Masculino , Pessoa de Meia-Idade , Phlebovirus/imunologia , Reação em Cadeia da Polimerase em Tempo Real , Trombocitopenia/epidemiologia , Trombocitopenia/virologia
8.
Plant Physiol Biochem ; 139: 342-349, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30952086

RESUMO

Melatonin (MEL) has been widely reported to be beneficial to plant growth and development, but few studies have combined investigations of the performance and function of MEL with detailed physiologically based analyses of nitrogen (N) uptake and metabolism in staple crops. In this study, the effect of MEL application on winter wheat seedling growth and grain yield were investigated in hydroponic and pot experiments at different N levels. The result showed that application of 1 µM MEL in hydroponic solution significantly improved the wheat seedling growth under both N sufficient and deficient conditions, but the effect of MEL on promoting seedling growth was prominent under N deficient condition. Meanwhile, MEL-treated plants maintained higher N contents and nitrate nitrogen levels in shoot under N deficient condition, and also maintained higher nitrate nitrogen levels in root. Further investigation showed that nitrate reductase (NR) and glutamine synthetase (GS) activities were higher in MEL-treated plants than that of MEL-untreated plants under N deficiency. The N absorption calculated based on N contents and biomass showed that MEL could promote the N absorption under N deficient condition. In pot experiment, pre-soaking of seeds with 100 µM MEL enhanced per-plant yield by 16% under N sufficient condition and 23% under N deficient condition. Taken together, the results of this study indicate that MEL is involved in promoting N uptake and assimilation through up-regulating the activities of N uptake and metabolism related enzymes and, ultimately, promotes the plant growth and yield, especially under N deficient condition.


Assuntos
Melatonina/metabolismo , Nitrogênio/metabolismo , Triticum/metabolismo , Glutamato-Amônia Ligase/metabolismo , Nitrato Redutase/metabolismo , Triticum/enzimologia
9.
Plant Sci ; 281: 52-60, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30824061

RESUMO

Carotenoids are required for many biological processes in plants and humans. Lycopene ε-cyclase (LCY-ε) catalyzes the conversion of lycopene into lutein via the α-branch carotenoid biosynthesis pathway. Down-regulation of IbLCY-ε by RNAi increases carotenoid accumulation and salt stress tolerance in transgenic sweetpotato calli. As the role of IbLCY-ε in carotenoid biosynthesis and environmental stress responses in whole plants is poorly understood, transgenic sweetpotato (RLE plants) with reduced expression of IbLCY-ε were developed. RLE plants contained higher levels of total carotenoid and ß-carotene, due to an elevated ß-carotene/lutein ratio rather than increased de novo biosynthesis. RLE plants showed high reactive oxygen species/radical-scavenging activity. They also exhibited an enhanced tolerance of both salt and drought stress, which was associated with lower membrane permeability and a higher photosynthetic rate, respectively. Elevated carotenoid accumulation in RLE plants mitigated the reductions in leaf photosystem II efficiency and chlorophyll induced by abiotic stress. Expression of the carotenoid cleavage genes 9-cis-epoxycarotenoid dioxygenase, carotenoid cleavage dioxygenase 1 (CCD1) and CCD4 was higher in RLE plants, as was abscisic acid accumulation. IbLCY-ε silencing thus offers an effective approach for developing sweetpotato plants with increased tolerance to abiotic stress that will grow on global marginal lands with no reduction in nutritional value.


Assuntos
Carotenoides/metabolismo , Liases Intramoleculares/metabolismo , Ipomoea batatas/metabolismo , Licopeno/metabolismo , Plantas Geneticamente Modificadas/metabolismo , beta Caroteno/metabolismo , Estresse Fisiológico
10.
Gut Pathog ; 10: 37, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30214488

RESUMO

Background: Culture-based diagnostic methods cannot achieve rapid and precise diagnoses for the identification of multiple diarrhoeal pathogens (DPs). A high-throughput multiplex genetic detection system (HMGS) was adapted and evaluated for the simultaneous identification and differentiation of infectious DPs and a broad analysis of DP infection aetiology. Results: DP-HMGS was highly sensitive and specific for DP detection compared with culture-based techniques and was similar to singleplex real-time PCR. The uniform level of sensitivity of DP-HMGS for all DPs allowed us to remap the aetiology of acute diarrhoeal infections in Shanghai, correcting incidences of massively underdiagnosed DP species with accuracy approaching that of sequencing-based methods. The most frequent DPs were enteropathogenic Escherichia coli, rotavirus and Campylobacter jejuni. DP-HMGS detected two additional causes of infectious diarrhoea that were previously missed by routine culture-based methods: enterohemorrhagic E. coli and Yersinia enterocolitica. We demonstrated the age dependence of specific DP distributions, especially the distributions of rotavirus, intestinal adenovirus and Clostridium difficile in paediatric patients as well as those of dominant bacterial infections in adults, with a distinct "top 3" pattern for each age group. Finally, the multiplexing capability and high sensitivity of DP-HMGS allowed the detection of infections co-induced by multiple pathogens (approximately 1/3 of the cases), with some DPs preferentially co-occurring as infectious agents. Conclusions: DP-HMGS has been shown to be a rapid, specific, sensitive and appropriate method for the simultaneous screening/detection of polymicrobial DP infections in faecal specimens. Widespread use of DP-HMGS is likely to advance routine diagnostic and clinical studies on the aetiology of acute diarrhoea.

11.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 43(8): 821-825, 2018 Aug 28.
Artigo em Chinês | MEDLINE | ID: mdl-30197308

RESUMO

OBJECTIVE: To determine the effect of andrographolide (Andro) on angiogenesis of human umbilical vein endothelial cells (HUVECs).
 Methods: HUVECs were treated with different concentrations of Andro and the cell viability was detected with Cell Counting Kit-8 (CCK-8). HUVECs were treated with half lethal dose (IC50) of Andro. Matrigel was used to make capillary formation of HUVECs and the effect of Andro on capillary formation was evaluated by calculating the percentage of capillary formation. Moreover, the effects of Andro and the supernatant from cultured A549 tumor cells on capillary formation were evaluated by calculating the percentage of capillary formation. The effect of Andro on the expression of matrix metalloproteinase-9 (MMP-9) was determined with Western blot.
 Results: The cell viability of HUVECs decreased with the increase of Andro concentrations. IC50 was 20 µmol/L. The capillary formation of HUVECs was inhibited when treated with 20 µmol/L Andro for 24 hours. Moreover, Andro was able to antagonize the promotion of the capillary formation induced by the supernatant from cultured tumor cells. Andro could suppress the expression of MMP-9 and antagonize the capillary formation.
 Conclusion: Andro inhibits the capillary formation of HUVECs and can antagonize the promotion of angiogenesis induced by the supernatant from cultured tumor cells.


Assuntos
Meios de Cultura , Diterpenos/farmacologia , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Neovascularização Patológica/prevenção & controle , Capilares/efeitos dos fármacos , Sobrevivência Celular , Colágeno , Combinação de Medicamentos , Humanos , Laminina , Metaloproteinase 9 da Matriz/metabolismo , Neovascularização Patológica/enzimologia , Neovascularização Patológica/etiologia , Proteoglicanas , Células Tumorais Cultivadas
12.
Virol Sin ; 33(4): 323-334, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30066045

RESUMO

This study aimed to investigate the serological characteristics of Ebola virus (EBOV) infection during the late phase of the Ebola outbreak in Sierra Leone. In total, 877 blood samples from 694 suspected Ebola virus disease (EVD) cases assessed from March to December 2015, were analyzed via real-time reverse transcription polymerase chain reaction (RT-PCR) for viral RNA and enzyme-linked immunosorbent assay (ELISA) and Luminex to detect antibodies against EBOV. Viral load and EBOV-specific IgM/IgG titers displayed a declining trend during March to December 2015. Viral RNA load decreased rapidly at earlier stages after disease onset, while EBOV-specific IgM and IgG still persisted in 58.1% (18/31) and 93.5% (29/31) of the confirmed EVD patients and in 3.8% (25/663) and 17.8% (118/663) of the RNA-negative suspected patients in the later phase, respectively. Dynamic analysis of longitudinally collected samples from eight EVD patients revealed typically reversed trends of declining viral load and increasing IgM and/or IgG titers in response to the EBOV infection. The present results indicate that certain populations of Sierra Leone developed immunity to an EBOV infection in the late phase of the outbreak, providing novel insights into the risk assessment of EBOV infections among human populations.


Assuntos
Surtos de Doenças , Ebolavirus/genética , Ebolavirus/imunologia , Doença pelo Vírus Ebola/sangue , Doença pelo Vírus Ebola/epidemiologia , Anticorpos Antivirais/sangue , Ensaio de Imunoadsorção Enzimática , Doença pelo Vírus Ebola/virologia , Humanos , RNA Viral/sangue , Reação em Cadeia da Polimerase em Tempo Real , Serra Leoa/epidemiologia , Fatores de Tempo , Carga Viral
13.
Front Plant Sci ; 9: 914, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30018628

RESUMO

Melatonin, a small molecular weight indoleamine molecule, is involved in various biological processes and responses to environmental cues in plants. However, its function in abiotic stress response and the underlying mechanisms is less clear. In this study, we investigated the effect of melatonin on wheat seedlings growth under salt stress condition. Exogenous melatonin pretreatment partially mitigated the salt-induced inhibition of whole-plant growth as judged from shoot dry weight, IAA content, leaf photosynthesis rate, maximum photochemistry efficiency of photosystem II, and chlorophyll. The mitigation was also observed in reduced accumulation of H2O2 in melatonin-pretreated wheat seedlings exposed to salt stress. Exogenous melatonin increased endogenous melatonin content by evaluating the levels of TaSNAT transcript, which encodes a key regulatory enzyme in the melatonin biosynthetic pathway. Furthermore, melatonin increased polyamine contents by accelerating the metabolic flow from the precursor amino acids arginine and methionine to polyamines; melatonin also decreased the degradation of salt-induced polyamines. Taken together, these results provide the evidence that melatonin mitigates salt stress mainly through its regulation on polyamine metabolism of wheat seedlings.

14.
J Pediatric Infect Dis Soc ; 7(3): e160-e162, 2018 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-29860410

RESUMO

Among newborns with congenital cytomegalovirus (CMV) infection from China, there was no difference in CMV viral load in saliva specimens dried and stored at room temperature compared with those kept wet and stored cold, even after longer storage time for the former than the later (74 vs 58 days, P = .02).


Assuntos
Infecções por Citomegalovirus/congênito , Infecções por Citomegalovirus/virologia , Saliva/virologia , Manejo de Espécimes/métodos , Carga Viral , China/epidemiologia , Infecções por Citomegalovirus/epidemiologia , Infecções por Citomegalovirus/imunologia , Teste em Amostras de Sangue Seco , Humanos , Recém-Nascido , Estudos Soroepidemiológicos , Fatores de Tempo
15.
Adv Mater ; 30(26): e1800762, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29761561

RESUMO

Although rechargeable aqueous zinc-ion batteries have attracted extensive interest due to their environmental friendliness and low cost, they still lack suitable cathodes with high rate capabilities, which are hampered by the intense charge repulsion of bivalent Zn2+ . Here, a novel intercalation pseudocapacitance behavior and ultrafast kinetics of Zn2+ into the unique tunnels of VO2 (B) nanofibers in aqueous electrolyte are demonstrated via in situ X-ray diffraction and various electrochemical measurements. Because VO2 (B) nanofibers possess unique tunnel transport pathways with big sizes (0.82 and 0.5 nm2 along the b- and c-axes) and little structural change on Zn2+ intercalation, the limitation from solid-state diffusion in the vanadium dioxide electrode is eliminated. Thus, VO2 (B) nanofibers exhibit a high reversible capacity of 357 mAh g-1 , excellent rate capability (171 mAh g-1 at 300 C), and high energy and power densities as applied for zinc-ion storage.

16.
Front Plant Sci ; 9: 281, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29556247

RESUMO

In plants, water deficiency can result from a deficit of water from the soil, an obstacle to the uptake of water or the excess water loss; in these cases, the similar consequence is the limitation of plant growth and crop yield. Silicon (Si) has been widely reported to alleviate the plant water status and water balance under variant stress conditions in both monocot and dicot plants, especially under drought and salt stresses. However, the underlying mechanism is unclear. In addition to the regulation of leaf transpiration, recently, Si application was found to be involved in the adjustment of root hydraulic conductance by up-regulating aquaporin gene expression and concentrating K in the xylem sap. Therefore, this review discusses the potential effects of Si on both leaf transpiration and root water absorption, especially focusing on how Si modulates the root hydraulic conductance. A growing number of studies support the conclusion that Si application improves plant water status by increasing root water uptake, rather than by decreasing their water loss under conditions of water deficiency. The enhancement of plant water uptake by Si is achievable through the activation of osmotic adjustment, improving aquaporin activity and increasing the root/shoot ratio. The underlying mechanisms of the Si on improving plant water uptake under water deficiency conditions are discussed.

17.
BMC Infect Dis ; 18(1): 66, 2018 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-29402229

RESUMO

BACKGROUND: Severe fever with thrombocytopenia syndrome (SFTS) is a severe viral disease caused by SFTSV. It is important to estimate the rate of missed SFTS diagnosis and to further understand the actual incidence in high endemic areas in China. METHODS: This study was conducted in two high SFTS endemic provinces in 2015. Patients hospitalized in 2014 or within 1 year before investigation were selected after considering their clinical manifestations, specifically, fever, platelet, and white blood cell. During retrospective investigation, sera were collected to detect SFTSV antibodies to assess SFTSV infection. To further understand SFTSV infection, acute phase sera were detected; SFTSV infection rate among a healthy population was also investigated to determine the basic infection level. RESULTS: In total, 246 hospitalized cases were included, including 83 cases (33.7%) with fever, thrombocytopenia and leukopenia, 38 cases (15.4%) with fever and thrombocytopenia but without leukopenia, and 125 cases (50.8%) without fever but with thrombocytopenia and leukopenia. In total, 13 patients (5.3%) were SFTSV IgM antibody-positive, 48 (19.5%) were IgG-positive. Of the 13 IgM-positive cases, 11 (84.6%) were IgG-positive (9 with titres ≥1:400). Seropositive rates of antibodies were high (8.4% for IgM and 30.1% for IgG) in patients with fever, thrombocytopenia and leukopenia. Furthermore, among IgG-positive cases in this group, 76% (19/25) of patients' IgG antibody titres were ≥1:400. Additionally, 28 of 246 cases were initially diagnosed with suspected SFTS and were then excluded, and 218 patients were never diagnosed with SFTS; the seropositive rates of IgM and IgG in these two groups were 25% and 67.9% and 2.8% and 13.3%, respectively. These rates were 64.3% and 21.4% in 14 sera collected during acute phase of the 28 cases mentioned above. Seropositive rate of SFTSV IgG was only 1.3% in the patient-matched healthy group, and no IgM antibody was detected. A preliminary estimate of 8.3% of SFTS cases were missed in SFTS high endemic provinces. CONCLUSIONS: The actual SFTS incidence was underestimated. Effective measures such as adding a new SFTS case category - "SFTS clinical diagnosis cases" or using serological detection methods during acute phase should be considered to avoid missed diagnoses.


Assuntos
Infecções por Bunyaviridae/epidemiologia , Febre/epidemiologia , Trombocitopenia/epidemiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticorpos Antivirais/sangue , China/epidemiologia , Feminino , Febre/complicações , Hospitalização , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Incidência , Leucopenia/complicações , Leucopenia/epidemiologia , Masculino , Pessoa de Meia-Idade , Phlebovirus/imunologia , Estudos Retrospectivos , Trombocitopenia/complicações , Adulto Jovem
18.
Int J Biol Sci ; 13(7): 868-877, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28808419

RESUMO

Vascular endothelial permeability transition does not cause significant lesions, but enhanced permeability may contribute to the development of vascular and other diseases, including atherosclerosis, hypertension, heart failure and cancer. Therefore, elucidating the effect of Particulate Matter 2.5 (PM2.5) on vascular endothelial permeability could help prevent disease that might be caused by PM2.5. Our previous study and the present one revealed that PM2.5 significantly increased the permeability of vascular endothelial cells and disrupted the barrier function of the vascular endothelium in Sprague Dawley (SD) rats. We found that the effect occurred mainly through induction of signal transducer and activator of transcription 3 (STAT3) phosphorylation, further transcriptional regulation of microRNA21 (miR-21) and promotion of miR-21 expression. These changes post-transcriptionally repress tissue inhibitor of metalloproteinases 3 (TIMP3) and promote matrix metalloproteinases 9 (MMP9) expression. This work provides evidence that PM2.5 exerts direct inhibitory action on vascular endothelial barrier function and might give rise to a number of vascular diseases.


Assuntos
Endotélio Vascular/efeitos dos fármacos , MicroRNAs/metabolismo , Material Particulado/toxicidade , Animais , Aorta Torácica , Caderinas/genética , Caderinas/metabolismo , Permeabilidade Capilar/efeitos dos fármacos , Regulação da Expressão Gênica/genética , Masculino , Metaloproteinase 9 da Matriz/genética , Metaloproteinase 9 da Matriz/metabolismo , MicroRNAs/genética , Tamanho da Partícula , Material Particulado/química , Ratos , Ratos Sprague-Dawley , Fator de Transcrição STAT3/genética , Fator de Transcrição STAT3/metabolismo , Inibidor Tecidual de Metaloproteinase-3/genética , Inibidor Tecidual de Metaloproteinase-3/metabolismo
19.
Nanoscale ; 9(28): 9811-9817, 2017 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-28696439

RESUMO

We describe an ultra-stable, ultra-robust, straightforward and low-cost approach for the colorimetric detection of H2S with nanocrystalline cellulose (NCC) based on the reaction of H2S with lead acetate. The presence of NCC not only mediates the seed growth of a PbS/NCC complex, but also acts as a stabilizer protecting PbS from precipitation. This stable system is so robust that it can be used to quantitatively detect H2S even after two-year storage.

20.
Biochem Biophys Res Commun ; 490(4): 1183-1188, 2017 09 02.
Artigo em Inglês | MEDLINE | ID: mdl-28669728

RESUMO

Inhibition of protein neddylation pathway has emerged an attractive anticancer strategy in preclinical studies by using Nedd8-activating enzyme (NAE) inhibitor MLN4924 (Pevonedistat). Previous studies have reported the antitumor activity of MLN4924 mediated by its efficacy on apoptosis, autophagy and senescence. However, whether MLN4924 has any effect on renal carcinoma cells (RCC) remains unexplored. Here we reported that MLN4924 specifically inhibited protein neddylation pathway, leading to statistically significantly suppress the proliferation, survival and migration of RCC cells by inducing G2 cell-cycle arrest, followed by apoptosis in a MLN4924 dose-dependent manner. Further mechanistic study revealed that MLN4924-induced apoptosis was mediated by substantial up-regulation of pro-apoptotic NOXA. These findings highlighted the anticancer effects of the neddylation inhibitors (e.g. MLN4924) for the treatment of RCC.


Assuntos
Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Carcinoma de Células Renais/tratamento farmacológico , Ciclopentanos/farmacologia , Neoplasias Renais/tratamento farmacológico , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Pirimidinas/farmacologia , Carcinoma de Células Renais/patologia , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Neoplasias Renais/patologia , Relação Estrutura-Atividade , Células Tumorais Cultivadas , Regulação para Cima/efeitos dos fármacos
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