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1.
Chin Med J (Engl) ; 132(12): 1441-1447, 2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31205102

RESUMO

BACKGROUND: Diagnosis of heparin-induced thrombocytopenia (HIT) is challenging. This study aimed to compare the diagnostic performance of HIT expert probability (HEP) and 4T scores, and to evaluate the inter-observer reliability for the 4T score in a clinical setting. METHODS: This prospective study included HIT-suspected patients between 2016 and 2018. Three hematologists assessed the HEP and 4T scores. Correlations between scores and anti-platelet factor 4 (anti-PF4)/heparin antibodies were evaluated. Receiver operating characteristic curves and area under the curve (AUC) were used to assess the predictive accuracy of these two scoring models. The intraclass correlation coefficient (ICC) was used to assess the inter-observer agreement of 4T scores between residents and hematologists. RESULTS: Of the 89 subjects included, 22 (24.7%) were positive for anti-PF4/heparin antibody. The correlations between antibody titer and either HEP or 4T scores were similar (r = 0.392, P < 0.01 for the HEP score; r = 0.444, P < 0.01 for the 4T score). No significant difference in the diagnostic performance was displayed between these two scores (AUC for the HEP score: 0.778 vs. AUC for the 4T score: 0.741, P = 0.357). Only 72 4T scores were collected from the residents, with a surprisingly low percentage of observers (43.1%) presenting the four individual item scores which made up their 4T score. The AUC of 4T score assessed by residents and hematologists was 0.657 (95% confidence interval [CI]: 536-0.765) and 0.780 (95% CI: 0.667-0.869, P < 0.05), respectively. The ICC of 4T score between residents and hematologists was 0.49 (95% CI: 0.29-0.65, P < 0.01), demonstrating a fair inter-observer agreement. CONCLUSIONS: The HEP score does not display a better performance for predicting HIT than the 4T score. With the unsatisfactory completion rate, the inter-observer agreement of 4T score in a tertiary hospital is fair, underscoring the necessity for continuing education for physicians.


Assuntos
Heparina/toxicidade , Trombocitopenia/induzido quimicamente , Trombocitopenia/diagnóstico , Adulto , Idoso , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Observacionais como Assunto , Estudos Prospectivos , Curva ROC , Centros de Atenção Terciária/estatística & dados numéricos
2.
J Int Med Res ; 47(4): 1810-1814, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30806107

RESUMO

OBJECTIVE: Kasabach-Merritt syndrome is a rare disease that mainly occurs in infants and adolescents. It usually manifests as disseminated intravascular coagulation and severe bleeding, and is associated with high mortality. However, its low incidence and clinical rarity in adults mean that there is currently no well-verified treatment regimen for this disease. We report on an effective novel therapeutic regimen in a patient with Kasabach-Merritt syndrome. METHODS: A woman with Kasabach-Merritt syndrome presented with a recurrent subcutaneous mass and disseminated intravascular coagulation, and was treated with prednisone, vincristine and thalidomide. RESULTS: This treatment regimen successfully resolved the patient's symptoms, with tumor regression. The patient remained disease-free after 6 years of follow-up. CONCLUSIONS: Prednisone combined with vincristine and thalidomide may be an effective treatment for Kasabach-Merritt syndrome, but further studies are needed to verify the use of this regimen.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Síndrome de Kasabach-Merritt/tratamento farmacológico , Adulto , Feminino , Humanos , Síndrome de Kasabach-Merritt/patologia , Prednisona/administração & dosagem , Prognóstico , Talidomida/administração & dosagem , Vincristina/administração & dosagem
3.
Medicine (Baltimore) ; 97(31): e11687, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30075565

RESUMO

Hereditary hemorrhagic telangiectasia (HHT) is a rare autosomal dominant disorder characterized by vascular dysplasia, including typically systemic telangiectases and arteriovenous malformations (AVMs). Due to its variable clinical manifestations, HHT patients often seek medical care from different medical subspecialties and thus experience delays in diagnosis and treatment.This study is designed to analyze the clinical features and treatment options for patients with HHT.Hospitalized patients with a definitive diagnosis of HHT from November 1973 to July 2016 in Peking Union Medical College Hospital were identified after reviewing medical records and electronic databases. Further follow-up data of these patients were collected from outpatient clinical visits and/or telephone interviews.We identified a total of 20 patients, 7 males and 13 females. The mean age was 42.4 ±â€Š20.3 years. Epistaxis (18/20) was the most common presentation, followed by telangiectases of the oral buccal mucosa, tongue and/or lips (14/20), pulmonary AVMs (12/19), hepatic AVMs (9/17), gastrointestinal telangiectases (9/9), and encephalic AVMs (1/12). The correct diagnosis of HHT was delayed on average by about 26.4 ±â€Š17.0 years from the onset of HHT-related clinical signs and symptoms. Although epistaxis is usually presented in childhood (mean age 11 ±â€Š7.1 years), gastrointestinal telangiectasia was often encountered in late middle age (mean age 55.4 ±â€Š12.8 years). Bleeding and anemia were the most common complications. Molecular analysis was conducted in 4 patients. Only 1 patient was found to have a single-base deletion in ENG gene. The mean duration of follow-up of the patients was 41.8 months. The efficacy of locoregional therapy was of limited value and short-lived. Two patients were treated systemically with thalidomide, and their symptoms of epistaxis, melena, and anemia were notably improved.Patients with HHT have variable clinical characteristics, and their diagnoses were delayed on average by about 26 years. An experienced multidisciplinary team is needed for the early diagnosis and optimal management of patients with HHT. Thalidomide may be an effective choice to alleviate the bleeding symptoms of patients with HHT.


Assuntos
Malformações Arteriovenosas/patologia , Telangiectasia Hemorrágica Hereditária/patologia , Adulto , Inibidores da Angiogênese/uso terapêutico , Malformações Arteriovenosas/tratamento farmacológico , Malformações Arteriovenosas/etiologia , Diagnóstico Tardio , Epistaxe/etiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/patologia , Telangiectasia Hemorrágica Hereditária/complicações , Telangiectasia Hemorrágica Hereditária/tratamento farmacológico , Telangiectasia/etiologia , Talidomida/uso terapêutico
4.
Int J Hematol ; 107(6): 615-623, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29619624

RESUMO

Primary immune thrombocytopenia (ITP) is a bleeding disorder commonly encountered in clinical practice. The International Working Group (IWG) on ITP has published several landmark papers on terminology, definitions, outcome criteria, bleeding assessment, diagnosis, and management of ITP. The Chinese consensus reports for diagnosis and management of adult ITP have been updated to the 4th edition. Based on current consensus positions and new emerging clinical evidence, the thrombosis and hemostasis group of the Chinese Society of Hematology issued Chinese guidelines for management of adult ITP, which aim to provide evidence-based recommendations for clinical decision making.


Assuntos
Medicina Baseada em Evidências , Hematologia/organização & administração , Guias de Prática Clínica como Assunto , Púrpura Trombocitopênica Idiopática/diagnóstico , Púrpura Trombocitopênica Idiopática/tratamento farmacológico , Sociedades Médicas/organização & administração , Idoso , China , Feminino , Humanos , Masculino , Índice de Gravidade de Doença
5.
Epigenomics ; 10(1): 43-57, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29172698

RESUMO

AIM: This study aimed to investigate the role of miRNAs in UGT1A regulation. MATERIALS & METHODS: Based on bioinformatic prediction results, luciferase reporter assay and cell-transfection experiments were performed to study effects of miR-298 on UGT1A expression. Correlation study was conducted in human livers. RESULTS: miR-298 overexpression reduced mRNA level of UGT1A1 and UGT1A4 in HepG2 and LS174T cells, and that of UGT1A3 and UGT1A9 in LS174T cells. miR-298 repression increased mRNA level of UGT1A4 in HepG2 and LS174T cells, and that of UGT1A1 and UGT1A3 in LS174T cells. Inverse correlations between miR-298, as well as miR-491-3p, and UGT1A3 and 1A4 mRNA levels were observed in livers. CONCLUSION: The study demonstrates that miR-298 and miR-491-3p downregulates UGT1A expression.


Assuntos
Glucuronosiltransferase/genética , Fígado/metabolismo , MicroRNAs/metabolismo , Grupo com Ancestrais do Continente Asiático , Linhagem Celular , Linhagem Celular Tumoral , Regulação para Baixo , Glucuronosiltransferase/metabolismo , Humanos
6.
Medicine (Baltimore) ; 96(31): e7557, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28767572

RESUMO

This study aimed to determine the relationship between the size of the cervical vertebral body and the morbidity of cervical spondylosis, and to examine the characteristics of spondylosis patients with small cervical vertebral bodies.The clinical data and the sagittal reconstructions of computed tomography images of 182 patients with cervical spondylosis were collected retrospectively. Patients included 74 males and 108 females, with a mean age of 31.8 years (range 20-40 years). The Torg-Pavlov ratio and the sagittal diameter of the vertebral body were measured. A Torg-Pavlov ratio above 1.2 was regarded as a small cervical vertebral body (SCVB), and below 1.2 as a nonsmall vertebral body (NSCVB).The NSCVB group was more prone to neurological symptoms than was the SCVB group (P < .05). There was no significant difference in neck pain between the 2 groups (P > .05). Conservative treatment achieved similar recovery rates in the SCVB group and the NSCVB group (81.8% vs 93.6%; P > .05). The rate of symptom (eg, axial neck pain) recurrence and persistence in the SCVB group was significantly higher than in the NSCVB group (P < .05).Our study found that smaller size of the cervical vertebral body is an attributing factor for cervical spondylosis. Patients with smaller cervical vertebral bodies are prone to persistent axial neck pain, but not neurological symptoms.


Assuntos
Vértebras Cervicais/diagnóstico por imagem , Espondilose/diagnóstico por imagem , Espondilose/mortalidade , Adulto , Tratamento Conservador , Feminino , Humanos , Masculino , Cervicalgia/diagnóstico por imagem , Cervicalgia/mortalidade , Cervicalgia/fisiopatologia , Cervicalgia/terapia , Tamanho do Órgão , Recidiva , Espondilose/fisiopatologia , Espondilose/terapia , Tomografia Computadorizada por Raios X , Resultado do Tratamento , Adulto Jovem
7.
Burns ; 43(7): 1449-1454, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28778757

RESUMO

BACKGROUND: The aim of this study is to analyze the data of amputees in the burn center of the Inner Mongolia region and to provide instructive suggestions for a preventative reduction of the amputation rate. METHODS: Between 2004 and 2016, all patient medical profiles were reviewed to extract data of patients with major amputation. Demographic data, mechanism of injury, location and level of amputation were recorded. The healing condition of the residual limb was noted. In addition, we performed comparisons of amputees whose injuries were caused by electricity and those whose injuries were related to other mechanisms. RESULTS: Among the 82 amputees in our study, about 89% of amputees were male patients and the predominant age-group was 20-29 years (26.8%). The injuries occurred most commonly at the work place (62.2%) with laborers (40.2%) being the most commonly affected. The most common mechanisms of injury were electricity (51.2%) and hot crush (14.6%), followed by frostbite (13.4%). The most common level of amputation was the right wrist joint (n=16). About 60.4% of the amputation sites were by primary healing. The rate of escharotomy in electrical burn amputees (n=27, 62.3%) was significantly higher than the other groups (n=16, 40.0%, p<0.05). The first amputation in electrical burn group (7.2±5.6) was significantly earlier than the other etiology group (17.9±13.7, p<0.05). CONCLUSIONS: Electrical burns were the major mechanism of injury among amputees. Effective safety measures, factory modifications, and adequate instructions should be implemented to protect laborers. Urgent interdisciplinary communication should be taken into account for the prevention reduction of the amputation rate in our region.


Assuntos
Amputação/estatística & dados numéricos , Queimaduras/cirurgia , Comunicação , Congelamento das Extremidades/cirurgia , Traumatismos da Mão/cirurgia , Traumatismos Ocupacionais/cirurgia , Equipe de Assistência ao Paciente , Adolescente , Adulto , Cotos de Amputação , Unidades de Queimados , Queimaduras/epidemiologia , Queimaduras por Corrente Elétrica/epidemiologia , Queimaduras por Corrente Elétrica/cirurgia , Criança , Pré-Escolar , China/epidemiologia , Feminino , Congelamento das Extremidades/epidemiologia , Traumatismos da Mão/epidemiologia , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Traumatismos Ocupacionais/epidemiologia , Estudos Retrospectivos , Cicatrização , Adulto Jovem
8.
Arch Virol ; 162(9): 2715-2726, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28578523

RESUMO

Porcine circovirus type 2 (PCV2) is the cause of postweaning multisystemic wasting syndrome (PMWS), which encompasses several distinct symptoms in pigs. PCV2 infection and clinical incidence of PMWS have increased in recent years, possibly due to shifts in viral populations and mutations. In this study, we identified PVC2 strains currently afflicting pig populations in mainland China, because this is a prerequisite for developing a specific vaccine to control the spread of PMWS. We collected 235 tissue samples from 16 provinces between 2014 and 2016. Of these, 152 samples were positive for PCV2. We compared the sequences we obtained for the PVC2 capsid gene, ORF2, to those of the Chinese PCV2 sequences deposited in GenBank between 2002 and 2016 (n = 648). Phylogenetic analyses demonstrated that the PCV2d genotype was the most prevalent strain in the sample population included in GenBank and among the positive samples from this study. We also found one PCV2c strain among the GenBank sequences. Furthermore, PCV2a-2F was the predominant genotype in the PCV2a cluster. Amino acid sequence comparisons demonstrated 70.8-100% identity within PCV ORF2 and several consistent mutations in ORF2. More interestingly, six isolates were classified as recombinant strains. Cumulatively, this study represents the first comprehensive description of PCV2 strains distribution, including recent samples, in Chinese porcine populations. We demonstrate the existence of high genetic variability among PVC2 strains and the ability of this virus to rapidly evolve.


Assuntos
Circovirus/genética , Síndrome Definhante Multissistêmico de Suínos Desmamados/virologia , Doenças dos Suínos/virologia , Animais , China/epidemiologia , Variação Genética , Genótipo , Mutação , Filogenia , Síndrome Definhante Multissistêmico de Suínos Desmamados/epidemiologia , Recombinação Genética , Suínos , Doenças dos Suínos/epidemiologia
9.
Clin Exp Rheumatol ; 35(3): 494-499, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28240594

RESUMO

OBJECTIVES: This study aimed to examine the long-term efficacy, remission and survival of patients with severe systemic lupus erythematosus (SLE) after the combination treatment with high-dose immunosuppressive therapy (HDIT) and autologous peripheral blood stem cell transplantation (APBSCT). METHODS: Chinese patients with severe SLE receiving combination therapy with HDIT and APBSCT in Peking Union Medical College Hospital were enrolled from July 1999 to October 2005. Disease activity, treatment, and adverse effects of these patients were evaluated. The 10-year overall survival and 10-year remission survival were also analysed. RESULTS: Among the 27 patients, one patient failed to collect enough CD34+ cells and data was missing for two patients. In the end, 24 patients were included in the final analysis. After APBSCT, one patient died, two patients achieved partial remission and 21 (87.5%) achieved remission at 6 months. The median follow-up duration of the 23 patients was 120 months. Fourteen patients had completed a ten-year follow-up. The median proteinuria level of the 14 patients with LN with ten years of follow-up significantly decreased from 4.00 g/24 hours at pre-treatment to 0.00g/24 hours at year 5 and 0.00 g/24 hours at year 10 (both p=0.001). The 10-year overall survival rate and 10-year remission survival rate were both 86.0% (95% CI: 71.1-100.9%). After a median follow-up for 120 months, 16 patients (66.7%) remained in remission, 4 patients were lost to follow-up, 2 patients died and 1 patient remained active. CONCLUSIONS: The combination of HDIT and APBSCT may be an option to improve the survival of severe lupus patients.


Assuntos
Imunossupressores/administração & dosagem , Nefrite Lúpica/terapia , Transplante de Células-Tronco de Sangue Periférico , Adolescente , Adulto , China , Feminino , Seguimentos , Humanos , Imunossupressores/efeitos adversos , Estimativa de Kaplan-Meier , Nefrite Lúpica/diagnóstico , Nefrite Lúpica/mortalidade , Masculino , Pessoa de Meia-Idade , Transplante de Células-Tronco de Sangue Periférico/efeitos adversos , Transplante de Células-Tronco de Sangue Periférico/mortalidade , Recidiva , Indução de Remissão , Índice de Gravidade de Doença , Fatores de Tempo , Transplante Autólogo , Resultado do Tratamento , Adulto Jovem
10.
Chin Med J (Engl) ; 130(2): 219-223, 2017 01 20.
Artigo em Inglês | MEDLINE | ID: mdl-28091415

RESUMO

OBJECTIVE: To provide a comprehensive literature review on roles of coagulation factor XIII (FXIII) in coagulation, wound healing, neoplasm, bone metabolism, and pregnancy. DATA SOURCES: All articles in PubMed with key words "Coagulation factor XIII", "wound", "leukemia", "tumor", "bone," and "pregnancy" with published date from 2001 to 2016 were included in the study. Frequently cited publications before 2000 were also included. STUDY SELECTION: We reviewed the role of FXIII in biologic processes as documented in clinical, animal, and in vitro studies. RESULTS: FXIII, a member of the transglutaminase (TG) family, plays key roles in various biological processes. Besides its well-known function in coagulation, the cross-linking of small molecules catalyzed by FXIII has been found in studies to help promote wound healing, improve bone metabolism, and prevent miscarriages. The study has also shown that FXIII concentration level differs in the blood of patients with leukemia and solid tumors and offers promises as a diagnostic indicator. CONCLUSIONS: FXIII has many more biologic functions besides being known as coagulation factor. The TG activity of FXIII contributes to several processes, including wound healing, bone extracellular matrix stabilization, and the interaction between embryo and decidua of uterus. Further research is needed to elucidate the link between FXIII and leukemia and solid tumors.


Assuntos
Fator XIII/metabolismo , Aborto Espontâneo/metabolismo , Aborto Espontâneo/prevenção & controle , Animais , Coagulação Sanguínea/fisiologia , Fator XIII/fisiologia , Feminino , Humanos , Leucemia/metabolismo , Gravidez , Cicatrização/fisiologia
11.
Eur J Clin Pharmacol ; 73(1): 29-37, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27704169

RESUMO

PURPOSE: Complete or partial inactivity of UGT1A1, the unique enzyme responsible for bilirubin glucuronidation, is commonly associated with hyperbilirubinemia. We investigated the dynamic expression of UGT1A1, and that of the transcription factors (TFs) involved in its developmental regulation, during human hepatic growth in Han Chinese individuals. METHODS: Eighty-eight prenatal, pediatric, and adult liver samples were obtained from Han Chinese individuals. Quantitative real-time polymerase chain reaction was used to evaluate mRNA expression of UGT1A1 and TFs including PXR, CAR, HNF1A, HNF4A, PPARA, etc. UGT1A1 protein levels and metabolic activity were determined by western blotting and high-performance liquid chromatography. Direct sequencing was employed to genotype UGT1A1*6 (211G˃A) and UGT1A1*28 (TA6˃TA7) polymorphisms. RESULTS: UGT1A1 expression was minimal in prenatal samples, but significantly elevated during pediatric and adult stages. mRNA and protein levels and metabolic activity were prominently increased (120-, 20-, and 10-fold, respectively) in pediatric and adult livers compared to prenatal samples. Furthermore, expression did not differ appreciably between pediatric and adult periods. Dynamic expression of TFs, including PXR, CAR, HNF1A, HNF4A, and PPARA, was consistent with UGT1A1 levels at each developmental stage. A pronounced correlation between expression of these TFs and that of UGT1A1 (P < 0.001) was observed. Moreover, UGT1A1*6 and UGT1A1*28 polymorphisms reduced levels of UGT1A1 by up to 40-60 %. CONCLUSIONS: Hepatic expression of transcription factors is associated with developmental regulation of UGT1A1 in the Han Chinese population. Moreover, UGT1A1 polymorphisms are associated with reduced expression of UGT1A1 mRNA and protein, as well as enzyme activity.


Assuntos
Glucuronosiltransferase/genética , Glucuronosiltransferase/metabolismo , Fígado/crescimento & desenvolvimento , Fígado/metabolismo , Fatores de Transcrição/genética , Adulto , Idoso , Grupo com Ancestrais do Continente Asiático/genética , Pré-Escolar , Feminino , Genótipo , Idade Gestacional , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Polimorfismo Genético , RNA Mensageiro/metabolismo
12.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 38(5): 543-547, 2016 10 10.
Artigo em Inglês | MEDLINE | ID: mdl-27825411

RESUMO

Objective To investigate the clinical features of patients with Castleman's disease (CD) and systemic lupus erythematosus (SLE). Methods According to the diagnostic information between 1994 to 2014 extracted from the database of the Medical Record Department of Peking Union Medical College Hospital (PUMCH),patients with CD and SLE were included. A thorough literature review utilizing the key words of "Castleman's disease","systemic lupus erythematosus","SLE",and "lupus" was performed in PubMed during the same period. Cases with detailed clinical information were included while cases without detailed information were excluded from the analysis of this study. Results Nine patients worldwide were available for analysis [2 cases from PUMCH,accounted for 0.03%(2/6502) of all patients diagnosed as SLE and 1.0% (2/100) of patients diagnosed as CD during the same period] with a male-to-female ratio of2:7. The median age at diagnosis of CD was 39.0 years (range:21- 60 years). All patients were diagnosed as multicentric CD with generalized peripheral lymphadenopathy. Pathologic examination showed a balanced distribution:plasma cell variant:hyaline-vascular variant:mixed variant=3:3:3. Fever was the most common symptom (88.9%,8/9). Blood system was the most commonly involved system (88.9%,8/9) and kidneys were the most commonly involved organ (88.9%,8/9). Autoimmune thrombocytopenia (AITP) was observed in 55.6% (5/9) of patients,which was significantly higher than the general SLE patients (15.0%) (P<0.01). None of the 9 patients had evidence of central nervous system involvement. Conclusions CD complicated by SLE is a rare clinical condition. Compared to the general SLE population,this subgroup of patients may have higher rate of AITP and lower rate of central nervous system involvement.


Assuntos
Hiperplasia do Linfonodo Gigante/fisiopatologia , Lúpus Eritematoso Sistêmico/complicações , Adulto , Hiperplasia do Linfonodo Gigante/complicações , Feminino , Humanos , Doenças Linfáticas/complicações , Masculino , Pessoa de Meia-Idade , Púrpura Trombocitopênica Idiopática/complicações , Adulto Jovem
13.
Drug Metab Pharmacokinet ; 31(6): 433-444, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27727071

RESUMO

CYP3A4 and CYP3A7 are generally served as the major adult and fetal liver forms, respectively, and exhibited a developmental switch during liver maturation. The objective of this study was to explore the potential mechanisms associated with the developmental switch of CYP3A4 and CYP3A7 in the Chinese Han population. We analyzed CYP3A4/7, nuclear receptors, and epigenetic modifications in human liver samples. We found that the expression levels of CYP3A4 mRNA in adults were significantly higher than the levels in fetus. In contrast, CYP3A7 mRNA expression reached a maximal level at an estimated gestational age of 25 weeks and then substantially decreased during the first year after birth. We also found that the expression level of hepatocyte nuclear factor 4 alpha (HNF4A) was most associated with CYP3A4 expression in adult liver; whereas the expression level of glucocorticoid receptor (GR) was intensively correlated with CYP3A7 expression in fetal liver. Furthermore, we illustrated the dynamic changes of H3K4me2 and H3K27me3 in the developmental switch of CYP3A7 and CYP3A4. In summary, our data suggested that HNF4A and GR, and epigenetic changes of H3K4me2 and H3K27me3 are associated with the ontogenic expressions of CYP3A4/3A7 in the livers of the Chinese Han population.


Assuntos
Citocromo P-450 CYP3A/genética , Regulação da Expressão Gênica no Desenvolvimento , Fígado/metabolismo , China , Citocromo P-450 CYP3A/metabolismo , Epigênese Genética , Feminino , Feto/metabolismo , Fator 4 Nuclear de Hepatócito/genética , Fator 4 Nuclear de Hepatócito/metabolismo , Histonas/metabolismo , Humanos , Fígado/embriologia , Fígado/crescimento & desenvolvimento , Masculino , Receptores Citoplasmáticos e Nucleares/genética , Receptores Citoplasmáticos e Nucleares/metabolismo , Receptores de Glucocorticoides/genética , Receptores de Glucocorticoides/metabolismo
14.
Virus Res ; 225: 33-39, 2016 10 02.
Artigo em Inglês | MEDLINE | ID: mdl-27619840

RESUMO

Currently, pseudorabies virus (PRV) variant strains are outbreaking in China; these variants belong to genotype II PRV. The traditional Bartha-K61 vaccine has failed to provide complete protection against the emergent variant strains. Therefore, rapid attenuation of current epidemic strains is needed for effective PRV control. In this study, we report a rapid method for editing the PRV genome using the CRISPR-Cas9 system. We developed a triple gE/gI/TK gene-inactivated HeN1 PRV strain, because mice were more susceptible to PRV infection, we then evaluated the attenuation of PRV in the mice and demonstrated that modified PRV was fully attenuated. Furthermore, the attenuated strain also induced immune protection in response to a parental PRV challenge. Overall, we showed that PRVs can be rapidly attenuated using CRISPR-Cas9 technology, which will be critical for PRV control, especially when new variant PRV strains emerge.


Assuntos
Sistemas CRISPR-Cas , Vetores Genéticos/genética , Herpesvirus Suídeo 1/genética , Vacinas contra Pseudorraiva/genética , Vacinas Atenuadas/genética , Animais , Feminino , Edição de Genes , Marcação de Genes , Herpesvirus Suídeo 1/imunologia , Camundongos , Vacinas contra Pseudorraiva/imunologia , RNA Guia , Deleção de Sequência , Vacinas Atenuadas/imunologia , Células Vero , Replicação Viral
15.
J Food Sci Technol ; 53(6): 2597-605, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27478215

RESUMO

This study aimed at evaluating the performance of peony seed oil microencapsulated by spray drying during encapsulation and storage. Four different combinations of gum arabic (GA), corn syrup (CS), whey protein concentrate (WPC) and sodium caseinate (CAS) were used to encapsulate peony seed oil. The best encapsulation efficiency was obtained for CAS/CS followed by the CAS/GA/CS combination with the encapsulation ratio of 93.71 and 92.80 %, respectively, while the lowest encapsulation efficiency was obtained for WPC/GA/CS (85.96 %). Scanning electron microscopy and confocal laser scanning microscopy revealed that the particles were spherical in shape and did not exhibit apparent cracks or fissures, and gum arabic was uniformly distributed across the wall of the microcapsules. Oxidative stability study indicated that the CAS/GA/CS combination presented the best protection against lipid oxidation and the smallest loss of polyunsaturated fatty acid content among all of the formulas as measured by gas chromatography. Therefore, CAS/GA/CS could be promising materials encapsulate peony seed oil with high encapsulation efficiency and minimal lipid oxidation.

17.
J Virol ; 89(20): 10712-6, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26246582

RESUMO

In China, a majority of the highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRSV) strains were seeded by the 2006 outbreak. However, the most recently emerged (2013-2014) HP-PRRSV strain has a very different genetic background. It is a NADC30-like PRRSV strain recently introduced from North America that has undergone genetic exchange with the classic HP-PRRSV strains in China. Subsequent isolation and characterization of this variant suggest high pathogenicity, so it merits special attention in control and vaccine strategies.


Assuntos
Genoma Viral , Síndrome Respiratória e Reprodutiva Suína/transmissão , Vírus da Síndrome Respiratória e Reprodutiva Suína/patogenicidade , Recombinação Genética , Sequência de Aminoácidos , Animais , China/epidemiologia , Dados de Sequência Molecular , América do Norte/epidemiologia , Filogenia , Síndrome Respiratória e Reprodutiva Suína/epidemiologia , Síndrome Respiratória e Reprodutiva Suína/mortalidade , Síndrome Respiratória e Reprodutiva Suína/virologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/classificação , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Análise de Sobrevida , Suínos , Virulência
18.
Virology ; 483: 32-43, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25965793

RESUMO

Recently pseudorabies outbreaks have occurred in many vaccinated farms in China. To identify genetic characteristics of pseudorabies virus (PRV) strains, we obtained the genomic sequences of PRV strains HeN1 and JS, which were compared to 4 PRV genomes and 729 partial gene sequences. PRV strains isolated in China showed marked sequence divergence compared to European and American strains. Phylogenetic analysis revealed that for the first time PRV can be divided into 2 distinct clusters, with Chinese strains being genotype II and PRVs isolated from other countries being genotype I. Restriction fragment length polymorphism analysis confirmed differences between HeN1 and Bartha strains, as did the presence of unique insertion/deletion polymorphisms and microsatellites. This divergence between the two genotypes may have been generated from long-term, independent evolution, which could also explain the low efficacy of the Bartha vaccine in protecting pigs infected with genotype II PRV.


Assuntos
Variação Genética , Herpesvirus Suídeo 1/classificação , Herpesvirus Suídeo 1/genética , Pseudorraiva/epidemiologia , Pseudorraiva/virologia , Animais , China/epidemiologia , Análise por Conglomerados , DNA Viral/química , DNA Viral/genética , Genoma Viral , Genótipo , Herpesvirus Suídeo 1/isolamento & purificação , Epidemiologia Molecular , Dados de Sequência Molecular , Filogenia , Análise de Sequência de DNA , Homologia de Sequência , Suínos
19.
J Huazhong Univ Sci Technolog Med Sci ; 35(2): 295-301, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25877368

RESUMO

The risk factors and precautions of inpatient suicide were explored. Thirty suicide victims were drawn from the adverse event reports of suicidal act during hospitalization in a general hospital from 2008 to 2014. Data were gathered from the focus group interviews of twelve nurses who had experienced inpatient suicide. The data were analyzed by using analytical technique based on grounded theory, and software QSR NVIVO8 was used to aid the collation of data. Three main themes of risk factors about inpatient suicide emerged from the analysis: individual value, social factors and environmental factors. The individual value was categorized into different groups such as sense of guilt, hopelessness and low self-esteem. Social factors included two aspects of negative life events and social support. Three themes of precautions about inpatient suicide appeared in this study: evaluation, nursing and information exchange. Evaluation was elaborated from both physical and psychological assessments. This finding extends existing work of risk factors and precautions about inpatient suicide and brings new knowledge about the reasons why inpatients commit suicide.


Assuntos
Pacientes Internados , Recursos Humanos de Enfermagem no Hospital/psicologia , Suicídio , Adulto , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pesquisa Qualitativa , Fatores de Risco
20.
Plant Cell ; 26(9): 3538-55, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25194029

RESUMO

The tubular growth of a pollen tube cell is crucial for the sexual reproduction of flowering plants. LePRK1 is a pollen-specific and plasma membrane-localized receptor-like kinase from tomato (Solanum lycopersicum). LePRK1 interacts with another receptor, LePRK2, and with KINASE PARTNER PROTEIN (KPP), a Rop guanine nucleotide exchange factor. Here, we show that pollen tubes overexpressing LePRK1 or a truncated LePRK1 lacking its extracellular domain (LePRK1ΔECD) have enlarged tips but also extend their leading edges by producing "blebs." Coexpression of LePRK1 and tomato PLIM2a, an actin bundling protein that interacts with KPP in a Ca(2+)-responsive manner, suppressed these LePRK1 overexpression phenotypes, whereas pollen tubes coexpressing KPP, LePRK1, and PLIM2a resumed the blebbing growth mode. We conclude that overexpression of LePRK1 or LePRK1ΔECD rewires pollen tube growth to a blebbing mode, through KPP- and PLIM2a-mediated bundling of actin filaments from tip plasma membranes. Arabidopsis thaliana pollen tubes expressing LePRK1ΔECD also grew by blebbing. Our results exposed a hidden capability of the pollen tube cell: upon overexpression of a single membrane-localized molecule, LePRK1 or LePRK1ΔECD, it can switch to an alternative mechanism for extension of the leading edge that is analogous to the blebbing growth mode reported for Dictyostelium and for Drosophila melanogaster stem cells.


Assuntos
Extensões da Superfície Celular/metabolismo , Lycopersicon esculentum/enzimologia , Lycopersicon esculentum/crescimento & desenvolvimento , Proteínas de Plantas/metabolismo , Tubo Polínico/enzimologia , Tubo Polínico/crescimento & desenvolvimento , Actinas/metabolismo , Regulação da Expressão Gênica de Plantas , Germinação , Lycopersicon esculentum/genética , Fenótipo , Proteínas de Plantas/química , Plantas Geneticamente Modificadas , Tubo Polínico/ultraestrutura , Ligação Proteica , Transporte Proteico
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